Belongs to the protein kinase superfamily
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 279 |
| Acc65I | GGTACC | 1 cut(s) | 329 |
| AccB1I | GGYRCC | 1 cut(s) | 329 |
| AciI | CCGC | 4 cut(s) | 362, 586, 754, 1125 |
| AclWI | GGATC | 2 cut(s) | 73, 131 |
| AcsI | RAATTY | 2 cut(s) | 164, 1056 |
| AcuI | CTGAAG | 1 cut(s) | 203 |
| AfaI | GTAC | 7 cut(s) | 63, 331, 373, 785, 1088, 1102, 1195 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 88, 381, 875 |
| AgsI | TTSAA | 5 cut(s) | 347, 893, 1055, 1071, 1223 |
| AhlI | ACTAGT | 1 cut(s) | 1136 |
| AjnI | CCWGG | 2 cut(s) | 399, 1096 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 522, 554 |
| AleI | CACNNNNGTG | 1 cut(s) | 774 |
| AluBI | AGCT | 9 cut(s) | 523, 548, 653, 673, 789, 896, 977, 999, 1182 |
| AluI | AGCT | 9 cut(s) | 523, 548, 653, 673, 789, 896, 977, 999, 1182 |
| Alw21I | GWGCWC | 3 cut(s) | 22, 445, 1051 |
| Alw26I | GTCTC | 2 cut(s) | 424, 678 |
| Alw44I | GTGCAC | 1 cut(s) | 18 |
| AlwI | GGATC | 2 cut(s) | 73, 131 |
| Ama87I | CYCGRG | 1 cut(s) | 13 |
| AoxI | GGCC | 1 cut(s) | 1010 |
| ApaLI | GTGCAC | 1 cut(s) | 18 |
| ApeKI | GCWGC | 4 cut(s) | 583, 734, 953, 1115 |
| ApoI | RAATTY | 2 cut(s) | 164, 1056 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 522, 618 |
| Asp718I | GGTACC | 1 cut(s) | 329 |
| AsuHPI | GGTGA | 3 cut(s) | 232, 911, 1042 |
| AsuII | TTCGAA | 1 cut(s) | 516 |
| AvaI | CYCGRG | 1 cut(s) | 13 |
| BaeGI | GKGCMC | 1 cut(s) | 22 |
| BanI | GGYRCC | 1 cut(s) | 329 |
| BauI | CACGAG | 1 cut(s) | 723 |
| BbsI | GAAGAC | 1 cut(s) | 401 |
| Bbv12I | GWGCWC | 3 cut(s) | 22, 445, 1051 |
| BbvI | GCAGC | 4 cut(s) | 595, 721, 965, 1102 |
| BccI | CCATC | 2 cut(s) | 203, 873 |
| BciT130I | CCWGG | 2 cut(s) | 401, 1098 |
| BcoDI | GTCTC | 2 cut(s) | 424, 678 |
| BcuI | ACTAGT | 1 cut(s) | 1136 |
| BfaI | CTAG | 5 cut(s) | 549, 833, 905, 1137, 1233 |
| BisI | GCNGC | 4 cut(s) | 584, 735, 954, 1116 |
| BlsI | GCNGC | 4 cut(s) | 585, 736, 955, 1117 |
| BmcAI | AGTACT | 1 cut(s) | 1088 |
| Bme1390I | CCNGG | 2 cut(s) | 401, 1098 |
| BmeT110I | CYCGRG | 1 cut(s) | 13 |
| BmiI | GGNNCC | 3 cut(s) | 301, 331, 830 |
| BmrFI | CCNGG | 2 cut(s) | 401, 1098 |
| BmsI | GCATC | 2 cut(s) | 1083, 1238 |
| BpiI | GAAGAC | 1 cut(s) | 401 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 48, 80, 706, 738 |
| Bpu14I | TTCGAA | 1 cut(s) | 516 |
| BsaJI | CCNNGG | 3 cut(s) | 333, 376, 493 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 88, 381, 875 |
| Bse1I | ACTGG | 2 cut(s) | 866, 1177 |
| BseBI | CCWGG | 2 cut(s) | 401, 1098 |
| BseDI | CCNNGG | 3 cut(s) | 333, 376, 493 |
| BseGI | GGATG | 2 cut(s) | 634, 884 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 88, 381, 875 |
| BseMII | CTCAG | 2 cut(s) | 451, 993 |
| BseNI | ACTGG | 2 cut(s) | 866, 1177 |
| BseRI | GAGGAG | 1 cut(s) | 1222 |
| BseSI | GKGCMC | 1 cut(s) | 22 |
| BseXI | GCAGC | 4 cut(s) | 595, 721, 965, 1102 |
| BseYI | CCCAGC | 1 cut(s) | 250 |
| BsgI | GTGCAG | 2 cut(s) | 291, 720 |
| BshFI | GGCC | 1 cut(s) | 1012 |
| BshNI | GGYRCC | 1 cut(s) | 329 |
| BsiHKAI | GWGCWC | 3 cut(s) | 22, 445, 1051 |
| BsiHKCI | CYCGRG | 1 cut(s) | 13 |
| BsiSI | CCGG | 1 cut(s) | 848 |
| BslFI | GGGAC | 1 cut(s) | 556 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 88, 381, 875 |
| BsmAI | GTCTC | 2 cut(s) | 424, 678 |
| BsmFI | GGGAC | 1 cut(s) | 556 |
| BsmI | GAATGC | 4 cut(s) | 104, 581, 801, 1142 |
| BsnI | GGCC | 1 cut(s) | 1012 |
| BsoBI | CYCGRG | 1 cut(s) | 13 |
| Bsp119I | TTCGAA | 1 cut(s) | 516 |
| Bsp1286I | GDGCHC | 3 cut(s) | 22, 445, 1051 |
| Bsp1407I | TGTACA | 1 cut(s) | 783 |
| Bsp143I | GATC | 3 cut(s) | 78, 123, 810 |
| Bsp19I | CCATGG | 1 cut(s) | 493 |
| BspACI | CCGC | 4 cut(s) | 362, 586, 754, 1125 |
| BspANI | GGCC | 1 cut(s) | 1012 |
| BspCNI | CTCAG | 2 cut(s) | 450, 994 |
| BspHI | TCATGA | 2 cut(s) | 526, 1203 |
| BspLI | GGNNCC | 3 cut(s) | 301, 331, 830 |
| BspPI | GGATC | 2 cut(s) | 73, 131 |
| BspT104I | TTCGAA | 1 cut(s) | 516 |
| BspT107I | GGYRCC | 1 cut(s) | 329 |
| BsrGI | TGTACA | 1 cut(s) | 783 |
| BsrI | ACTGG | 2 cut(s) | 866, 1177 |
| BssECI | CCNNGG | 3 cut(s) | 333, 376, 493 |
| BssMI | GATC | 3 cut(s) | 78, 123, 810 |
| BssSI | CACGAG | 1 cut(s) | 723 |
| BssT1I | CCWWGG | 2 cut(s) | 376, 493 |
| Bst2BI | CACGAG | 1 cut(s) | 723 |
| Bst2UI | CCWGG | 2 cut(s) | 401, 1098 |
| Bst4CI | ACNGT | 1 cut(s) | 24 |
| BstAUI | TGTACA | 1 cut(s) | 783 |
| BstBI | TTCGAA | 1 cut(s) | 516 |
| BstC8I | GCNNGC | 2 cut(s) | 581, 752 |
| BstDEI | CTNAG | 2 cut(s) | 437, 1002 |
| BstDSI | CCRYGG | 1 cut(s) | 493 |
| BstF5I | GGATG | 2 cut(s) | 634, 884 |
| BstKTI | GATC | 3 cut(s) | 81, 126, 813 |
| BstMAI | GTCTC | 2 cut(s) | 424, 678 |
| BstMBI | GATC | 3 cut(s) | 78, 123, 810 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1124 |
| BstNI | CCWGG | 2 cut(s) | 401, 1098 |
| BstSCI | CCNGG | 2 cut(s) | 399, 1096 |
| BstSLI | GKGCMC | 1 cut(s) | 22 |
| BstV1I | GCAGC | 4 cut(s) | 595, 721, 965, 1102 |
| BstV2I | GAAGAC | 1 cut(s) | 401 |
| BstX2I | RGATCY | 2 cut(s) | 78, 123 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 250 |
| BstYI | RGATCY | 2 cut(s) | 78, 123 |
| BsuRI | GGCC | 1 cut(s) | 1012 |
| BtgI | CCRYGG | 1 cut(s) | 493 |
| BtsCI | GGATG | 2 cut(s) | 634, 884 |
| BtsI | GCAGTG | 2 cut(s) | 318, 963 |
| BtsIMutI | CAGTG | 3 cut(s) | 318, 963, 1149 |
| Cac8I | GCNNGC | 2 cut(s) | 581, 752 |
| CciI | TCATGA | 2 cut(s) | 526, 1203 |
| CsiI | ACCWGGT | 1 cut(s) | 399 |
| Csp6I | GTAC | 7 cut(s) | 62, 330, 372, 784, 1087, 1101, 1194 |
| CviAII | CATG | 5 cut(s) | 358, 413, 494, 527, 1204 |
| CviQI | GTAC | 7 cut(s) | 62, 330, 372, 784, 1087, 1101, 1194 |
| DdeI | CTNAG | 2 cut(s) | 437, 1002 |
| DpnI | GATC | 3 cut(s) | 80, 125, 812 |
| DpnII | GATC | 3 cut(s) | 78, 123, 810 |
| EciI | GGCGGA | 1 cut(s) | 377 |
| Eco130I | CCWWGG | 2 cut(s) | 376, 493 |
| Eco147I | AGGCCT | 1 cut(s) | 1012 |
| Eco57I | CTGAAG | 1 cut(s) | 203 |
| Eco88I | CYCGRG | 1 cut(s) | 13 |
| EcoRII | CCWGG | 2 cut(s) | 399, 1096 |
| EcoT14I | CCWWGG | 2 cut(s) | 376, 493 |
| ErhI | CCWWGG | 2 cut(s) | 376, 493 |
| FaeI | CATG | 5 cut(s) | 361, 416, 497, 530, 1207 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1035, 1067 |
| FaqI | GGGAC | 1 cut(s) | 556 |
| FatI | CATG | 5 cut(s) | 357, 412, 493, 526, 1203 |
| FauI | CCCGC | 1 cut(s) | 1132 |
| Fnu4HI | GCNGC | 4 cut(s) | 584, 735, 954, 1116 |
| FokI | GGATG | 2 cut(s) | 641, 891 |
| Fsp4HI | GCNGC | 4 cut(s) | 584, 735, 954, 1116 |
| FspBI | CTAG | 5 cut(s) | 549, 833, 905, 1137, 1233 |
| GluI | GCNGC | 4 cut(s) | 584, 735, 954, 1116 |
| GsaI | CCCAGC | 1 cut(s) | 254 |
| HaeIII | GGCC | 1 cut(s) | 1012 |
| HapII | CCGG | 1 cut(s) | 848 |
| Hin1II | CATG | 5 cut(s) | 361, 416, 497, 530, 1207 |
| HincII | GTYRAC | 1 cut(s) | 634 |
| HindII | GTYRAC | 1 cut(s) | 634 |
| HindIII | AAGCTT | 1 cut(s) | 521 |
| HinfI | GANTC | 8 cut(s) | 112, 148, 385, 513, 691, 841, 853, 1154 |
| HpaI | GTTAAC | 1 cut(s) | 634 |
| HpaII | CCGG | 1 cut(s) | 848 |
| HphI | GGTGA | 3 cut(s) | 232, 911, 1042 |
| Hpy166II | GTNNAC | 5 cut(s) | 20, 595, 634, 784, 902 |
| Hpy188I | TCNGA | 9 cut(s) | 35, 117, 130, 147, 200, 222, 337, 440, 1003 |
| Hpy188III | TCNNGA | 4 cut(s) | 258, 527, 857, 1204 |
| Hpy8I | GTNNAC | 5 cut(s) | 20, 595, 634, 784, 902 |
| HpyAV | CCTTC | 5 cut(s) | 102, 310, 816, 1037, 1077 |
| HpyCH4III | ACNGT | 1 cut(s) | 24 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1124 |
| HpyF3I | CTNAG | 2 cut(s) | 437, 1002 |
| Hsp92II | CATG | 5 cut(s) | 361, 416, 497, 530, 1207 |
| KpnI | GGTACC | 1 cut(s) | 333 |
| KspAI | GTTAAC | 1 cut(s) | 634 |
| Kzo9I | GATC | 3 cut(s) | 78, 123, 810 |
| LmnI | GCTCC | 1 cut(s) | 1046 |
| Lsp1109I | GCAGC | 4 cut(s) | 595, 721, 965, 1102 |
| LweI | GCATC | 2 cut(s) | 1083, 1238 |
| MabI | ACCWGGT | 1 cut(s) | 399 |
| MaeI | CTAG | 5 cut(s) | 549, 833, 905, 1137, 1233 |
| MaeIII | GTNAC | 1 cut(s) | 8 |
| MalI | GATC | 3 cut(s) | 80, 125, 812 |
| MboI | GATC | 3 cut(s) | 78, 123, 810 |
| MboII | GAAGA | 6 cut(s) | 88, 359, 401, 584, 895, 920 |
| MflI | RGATCY | 2 cut(s) | 78, 123 |
| MhlI | GDGCHC | 3 cut(s) | 22, 445, 1051 |
| MluCI | AATT | 7 cut(s) | 164, 342, 353, 531, 1056, 1071, 1218 |
| MlyI | GAGTC | 3 cut(s) | 142, 685, 862 |
| MmeI | TCCRAC | 1 cut(s) | 702 |
| MroXI | GAANNNNTTC | 2 cut(s) | 522, 618 |
| MseI | TTAA | 3 cut(s) | 171, 633, 1074 |
| MslI | CAYNNNNRTG | 1 cut(s) | 774 |
| MspA1I | CMGCKG | 1 cut(s) | 586 |
| MspI | CCGG | 1 cut(s) | 848 |
| MspR9I | CCNGG | 2 cut(s) | 401, 1098 |
| Mva1269I | GAATGC | 4 cut(s) | 104, 581, 801, 1142 |
| MvaI | CCWGG | 2 cut(s) | 401, 1098 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1124 |
| NcoI | CCATGG | 1 cut(s) | 493 |
| NdeII | GATC | 3 cut(s) | 78, 123, 810 |
| NlaIII | CATG | 5 cut(s) | 361, 416, 497, 530, 1207 |
| NlaIV | GGNNCC | 3 cut(s) | 301, 331, 830 |
| NspV | TTCGAA | 1 cut(s) | 516 |
| OliI | CACNNNNGTG | 1 cut(s) | 774 |
| PagI | TCATGA | 2 cut(s) | 526, 1203 |
| PceI | AGGCCT | 1 cut(s) | 1012 |
| PctI | GAATGC | 4 cut(s) | 104, 581, 801, 1142 |
| PdmI | GAANNNNTTC | 2 cut(s) | 522, 618 |
| PfeI | GAWTC | 5 cut(s) | 112, 385, 513, 841, 1154 |
| PkrI | GCNGC | 4 cut(s) | 585, 736, 955, 1117 |
| PleI | GAGTC | 3 cut(s) | 142, 685, 861 |
| PpsI | GAGTC | 3 cut(s) | 142, 685, 861 |
| PsiI | TTATAA | 1 cut(s) | 279 |
| Psp6I | CCWGG | 2 cut(s) | 399, 1096 |
| PspFI | CCCAGC | 1 cut(s) | 250 |
| PspGI | CCWGG | 2 cut(s) | 399, 1096 |
| PspN4I | GGNNCC | 3 cut(s) | 301, 331, 830 |
| PsuI | RGATCY | 2 cut(s) | 78, 123 |
| RsaI | GTAC | 7 cut(s) | 63, 331, 373, 785, 1088, 1102, 1195 |
| RsaNI | GTAC | 7 cut(s) | 62, 330, 372, 784, 1087, 1101, 1194 |
| RseI | CAYNNNNRTG | 1 cut(s) | 774 |
| SaqAI | TTAA | 3 cut(s) | 171, 633, 1074 |
| SatI | GCNGC | 4 cut(s) | 584, 735, 954, 1116 |
| Sau3AI | GATC | 3 cut(s) | 78, 123, 810 |
| ScaI | AGTACT | 1 cut(s) | 1088 |
| SchI | GAGTC | 3 cut(s) | 142, 685, 862 |
| ScrFI | CCNGG | 2 cut(s) | 401, 1098 |
| SduI | GDGCHC | 3 cut(s) | 22, 445, 1051 |
| SexAI | ACCWGGT | 1 cut(s) | 399 |
| SfaNI | GCATC | 2 cut(s) | 1083, 1238 |
| SfuI | TTCGAA | 1 cut(s) | 516 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 774 |
| SpeI | ACTAGT | 1 cut(s) | 1136 |
| Sse9I | AATT | 7 cut(s) | 164, 342, 353, 531, 1056, 1071, 1218 |
| SseBI | AGGCCT | 1 cut(s) | 1012 |
| SsiI | CCGC | 4 cut(s) | 362, 586, 754, 1125 |
| SspI | AATATT | 3 cut(s) | 87, 288, 619 |
| SspMI | CTAG | 5 cut(s) | 549, 833, 905, 1137, 1233 |
| StuI | AGGCCT | 1 cut(s) | 1012 |
| StyD4I | CCNGG | 2 cut(s) | 399, 1096 |
| StyI | CCWWGG | 2 cut(s) | 376, 493 |
| TaaI | ACNGT | 1 cut(s) | 24 |
| TaqI | TCGA | 2 cut(s) | 516, 1146 |
| TasI | AATT | 7 cut(s) | 164, 342, 353, 531, 1056, 1071, 1218 |
| TatI | WGTACW | 3 cut(s) | 61, 783, 1086 |
| TfiI | GAWTC | 5 cut(s) | 112, 385, 513, 841, 1154 |
| Tru1I | TTAA | 3 cut(s) | 171, 633, 1074 |
| Tru9I | TTAA | 3 cut(s) | 171, 633, 1074 |
| TscAI | CASTG | 3 cut(s) | 318, 963, 1156 |
| TseI | GCWGC | 4 cut(s) | 583, 734, 953, 1115 |
| TspDTI | ATGAA | 7 cut(s) | 173, 346, 377, 401, 515, 543, 951 |
| TspRI | CASTG | 3 cut(s) | 318, 963, 1156 |
| VneI | GTGCAC | 1 cut(s) | 18 |
| XapI | RAATTY | 2 cut(s) | 164, 1056 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 876 |
| XmnI | GAANNNNTTC | 2 cut(s) | 522, 618 |
| XspI | CTAG | 5 cut(s) | 549, 833, 905, 1137, 1233 |
| ZrmI | AGTACT | 1 cut(s) | 1088 |