Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 166 |
| AcoI | YGGCCR | 1 cut(s) | 357 |
| AfaI | GTAC | 3 cut(s) | 61, 146, 173 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 399, 400 |
| AgsI | TTSAA | 3 cut(s) | 85, 167, 209 |
| AluBI | AGCT | 2 cut(s) | 214, 344 |
| AluI | AGCT | 2 cut(s) | 214, 344 |
| Alw21I | GWGCWC | 1 cut(s) | 133 |
| AlwI | GGATC | 1 cut(s) | 166 |
| AoxI | GGCC | 1 cut(s) | 357 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 213, 309 |
| AsuC2I | CCSGG | 1 cut(s) | 416 |
| BalI | TGGCCA | 1 cut(s) | 359 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 7, 39 |
| Bbv12I | GWGCWC | 1 cut(s) | 133 |
| BccI | CCATC | 1 cut(s) | 84 |
| BciVI | GTATCC | 1 cut(s) | 16 |
| BcnI | CCSGG | 1 cut(s) | 416 |
| BfuI | GTATCC | 1 cut(s) | 16 |
| BmcAI | AGTACT | 1 cut(s) | 173 |
| Bme1390I | CCNGG | 1 cut(s) | 416 |
| BmrFI | CCNGG | 1 cut(s) | 416 |
| BmsI | GCATC | 1 cut(s) | 310 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 396, 428 |
| BpuMI | CCSGG | 1 cut(s) | 416 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 399, 400 |
| BseGI | GGATG | 1 cut(s) | 325 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 399, 400 |
| BseMII | CTCAG | 1 cut(s) | 139 |
| BsgI | GTGCAG | 1 cut(s) | 410 |
| BshFI | GGCC | 1 cut(s) | 359 |
| BsiHKAI | GWGCWC | 1 cut(s) | 133 |
| BsiSI | CCGG | 1 cut(s) | 415 |
| BslFI | GGGAC | 2 cut(s) | 247, 423 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 399, 400 |
| BsmFI | GGGAC | 2 cut(s) | 247, 423 |
| BsmI | GAATGC | 1 cut(s) | 272 |
| BsnI | GGCC | 1 cut(s) | 359 |
| Bsp1286I | GDGCHC | 1 cut(s) | 133 |
| Bsp143I | GATC | 1 cut(s) | 158 |
| BspANI | GGCC | 1 cut(s) | 359 |
| BspCNI | CTCAG | 1 cut(s) | 138 |
| BspHI | TCATGA | 1 cut(s) | 217 |
| BspPI | GGATC | 1 cut(s) | 166 |
| BssMI | GATC | 1 cut(s) | 158 |
| BstDEI | CTNAG | 1 cut(s) | 125 |
| BstF5I | GGATG | 1 cut(s) | 325 |
| BstKTI | GATC | 1 cut(s) | 161 |
| BstMBI | GATC | 1 cut(s) | 158 |
| BstSCI | CCNGG | 1 cut(s) | 414 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 193 |
| BsuI | GTATCC | 1 cut(s) | 16 |
| BsuRI | GGCC | 1 cut(s) | 359 |
| BtsCI | GGATG | 1 cut(s) | 325 |
| CciI | TCATGA | 1 cut(s) | 217 |
| Csp6I | GTAC | 3 cut(s) | 60, 145, 172 |
| CviAII | CATG | 3 cut(s) | 148, 190, 218 |
| CviJI | RGCY | 5 cut(s) | 214, 301, 344, 359, 441 |
| CviKI_1 | RGCY | 5 cut(s) | 214, 301, 344, 359, 441 |
| CviQI | GTAC | 3 cut(s) | 60, 145, 172 |
| DdeI | CTNAG | 1 cut(s) | 125 |
| DpnI | GATC | 1 cut(s) | 160 |
| DpnII | GATC | 1 cut(s) | 158 |
| EaeI | YGGCCR | 1 cut(s) | 357 |
| FaeI | CATG | 3 cut(s) | 151, 193, 221 |
| FaqI | GGGAC | 2 cut(s) | 247, 423 |
| FatI | CATG | 3 cut(s) | 147, 189, 217 |
| FokI | GGATG | 1 cut(s) | 332 |
| HaeIII | GGCC | 1 cut(s) | 359 |
| HapII | CCGG | 1 cut(s) | 415 |
| Hin1II | CATG | 3 cut(s) | 151, 193, 221 |
| HindIII | AAGCTT | 1 cut(s) | 212 |
| HinfI | GANTC | 3 cut(s) | 30, 246, 411 |
| HpaII | CCGG | 1 cut(s) | 415 |
| Hpy166II | GTNNAC | 1 cut(s) | 139 |
| Hpy188I | TCNGA | 4 cut(s) | 22, 52, 68, 128 |
| Hpy188III | TCNNGA | 1 cut(s) | 218 |
| Hpy8I | GTNNAC | 1 cut(s) | 139 |
| HpyCH4V | TGCA | 3 cut(s) | 259, 323, 427 |
| HpyF3I | CTNAG | 1 cut(s) | 125 |
| Hsp92II | CATG | 3 cut(s) | 151, 193, 221 |
| Kzo9I | GATC | 1 cut(s) | 158 |
| LpnPI | CCDG | 6 cut(s) | 74, 315, 358, 384, 407, 428 |
| LweI | GCATC | 1 cut(s) | 310 |
| MaeIII | GTNAC | 2 cut(s) | 290, 368 |
| MalI | GATC | 1 cut(s) | 160 |
| MboI | GATC | 1 cut(s) | 158 |
| MboII | GAAGA | 2 cut(s) | 81, 275 |
| MhlI | GDGCHC | 1 cut(s) | 133 |
| MlsI | TGGCCA | 1 cut(s) | 359 |
| MluCI | AATT | 2 cut(s) | 41, 279 |
| MluNI | TGGCCA | 1 cut(s) | 359 |
| MlyI | GAGTC | 1 cut(s) | 405 |
| MmeI | TCCRAC | 1 cut(s) | 30 |
| MnlI | CCTC | 4 cut(s) | 109, 134, 236, 400 |
| Mox20I | TGGCCA | 1 cut(s) | 359 |
| MroXI | GAANNNNTTC | 2 cut(s) | 213, 309 |
| MscI | TGGCCA | 1 cut(s) | 359 |
| Msp20I | TGGCCA | 1 cut(s) | 359 |
| MspI | CCGG | 1 cut(s) | 415 |
| MspR9I | CCNGG | 1 cut(s) | 416 |
| Mva1269I | GAATGC | 1 cut(s) | 272 |
| NciI | CCSGG | 1 cut(s) | 416 |
| NdeII | GATC | 1 cut(s) | 158 |
| NlaIII | CATG | 3 cut(s) | 151, 193, 221 |
| NmuCI | GTSAC | 1 cut(s) | 290 |
| PagI | TCATGA | 1 cut(s) | 217 |
| PctI | GAATGC | 1 cut(s) | 272 |
| PdmI | GAANNNNTTC | 2 cut(s) | 213, 309 |
| PfeI | GAWTC | 2 cut(s) | 30, 246 |
| PleI | GAGTC | 1 cut(s) | 405 |
| PpsI | GAGTC | 1 cut(s) | 405 |
| RsaI | GTAC | 3 cut(s) | 61, 146, 173 |
| RsaNI | GTAC | 3 cut(s) | 60, 145, 172 |
| Sau3AI | GATC | 1 cut(s) | 158 |
| ScaI | AGTACT | 1 cut(s) | 173 |
| SchI | GAGTC | 1 cut(s) | 405 |
| ScrFI | CCNGG | 1 cut(s) | 416 |
| SduI | GDGCHC | 1 cut(s) | 133 |
| SetI | ASST | 3 cut(s) | 126, 216, 346 |
| SfaNI | GCATC | 1 cut(s) | 310 |
| Sse9I | AATT | 2 cut(s) | 41, 279 |
| StyD4I | CCNGG | 1 cut(s) | 414 |
| TasI | AATT | 2 cut(s) | 41, 279 |
| TatI | WGTACW | 3 cut(s) | 59, 144, 171 |
| TfiI | GAWTC | 2 cut(s) | 30, 246 |
| TseFI | GTSAC | 1 cut(s) | 290 |
| Tsp45I | GTSAC | 1 cut(s) | 290 |
| TspDTI | ATGAA | 3 cut(s) | 170, 206, 275 |
| XmnI | GAANNNNTTC | 2 cut(s) | 213, 309 |
| ZrmI | AGTACT | 1 cut(s) | 173 |