Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0000690

Physical Location & Seq

Genomic Coordinates Forward (+)

26647831 .. 26649607

Gene Structure 1777 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1251000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1308 bp

ATGTCGAGTCCAGATTCATCATCATCATCATCAAGTGGTGGAGAAACAGCAGCTATTGCTATAGCCATGGTGGTGATCTTAGTCAAGATCGTTATCGCAATTGCAGTGTGTCAGAAATGTAAGGAAATAGCAGAAAAGAAACGAATTCAGAATAGTAGCTTAGTGAAGATCGCAAGTCCAGACTTTCGGGCACTCACAATGGACAACTTCCTAAATGATATGGAAAGAGAGAAGCCCATCAGGTTCACTTCTCAACAGCTCCGGATTGCTACCGATAACTTCACTAATCTGTTGGGCCAAGGAGGATTTGGTGCAGTTTATAAAGGAATATTTAGCAATGGAACTCTTGTAGCAGTGAAGGTTCTATATGGAAGTTCGGATAAGAGAATCAAGGAACAATTTATGGCGGAAGTTAGTACAATCGGAAGAACCCATCACTTCAATCTGGTCCGTCTTTATGGTTTCTGCTTCGAGGAACACCTCAGAGCACTTGTTTACGAGTACATGGGAAATGGATCGCTTGACAAGTACTTATTTAGTACCAACACGGTGGCGTTAGGATTTGAAAAGCTTCATGAGATTGCTGTGGGGACAGCAAGAGGGATCGCTTACTTGCACGAAGAATGCCAACAGCGGATTGTCCATTACGACATAAAGCCTGAAAATATTCTATTGGATGCAAACTTCTTTCCAAAAGTAGCTGACTTCGGTTTGGCCAAGTTGTGTAACAGGGAAAATACTCATATAACCATGACAGGGGGGAGAGGGACTCCGGGTTATGCTGCACCGGAAGTTTGGCTGAGGTTTCCTATAACACATAAATGTGATGTCTACAGCTTTGGAATGCTATTGTTCGAGATCATAGGAAGGAGAAGGAATCTCGACGTCAATAGTCAAGATAGCCAAGATTGGTTCCCGAGGTGGGTATGGAAGAAGTTTGAACCGGGGGAATTAGGAGAGCTAATGGTAGTTTGTGGAATAGAGGAGAAAGATAAAGAGAGGGCAGAGAGAATGGTAAAGTTAGCTCTGTGGTGTGTACAGTACATGCCCGAGGCAAGGCCTTTGATGAGTATCGTTGTTAAAATGTTGGAAGGTGCAGTTGAGATTCCTACACCTTCAACTAACCCTTTCCAGCACTTGATGTCGGACACTCCGTACCCTACTGCACCTGTCTATGATACTTCAAATCCAACAGACAGTACCGCCAGTGATTTTTGTTCGGTTCCTTATCAAACTGTAACTGGACATAGCGTCGTCAGTGGTACTCCTATCATGAAGAAATATGAGATTGAAATAGTGTCTGCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

436

Amino Acids

48.68

Weight (kDa)

6.39

Isoelectric Point (pI)

41.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	94 - 362	1.7e-45	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	96 - 355	2.6e-44	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000690)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g07910
malus_domestica	MD10G1181800.v1.1 MD10G1181900.v1.1 MD13G1249800.v1.1 MD13G1250100.v1.1 MD13G1250500.v1.1 MD13G1250700.v1.1 MD13G1250900.v1.1 MD13G1251000.v1.1 MD16G1220300.v1.1
prunus_persica	Prupe.1G082900_v2.0.a1 Prupe.1G083000_v2.0.a1 Prupe.1G083000_v2.0.a1 Prupe.1G083100_v2.0.a1 Prupe.1G083300_v2.0.a1
pyrus_communis	pycom16g18410 pycom16g18420 pycom16g18430
rosa_chinensis	RchiOBHm_Chr4g0401211 RchiOBHm_Chr4g0401231 RchiOBHm_Chr4g0401251 RchiOBHm_Chr4g0401331 RchiOBHm_Chr4g0401351 RchiOBHm_Chr4g0401361 RchiOBHm_Chr4g0401381 RchiOBHm_Chr4g0401471 RchiOBHm_Chr4g0401481 RchiOBHm_Chr4g0401501 RchiOBHm_Chr4g0401511 RchiOBHm_Chr4g0401631 RchiOBHm_Chr4g0401651
rosa_laevigata	RLG00000009145 RLG00000009146 RLG00000009148 RLG00000009149 RLG00000009151 RLG00000009154 RLG00000009155
rosa_multiflora	Rmu_sc0001524.1_g000025 Rmu_sc0001524.1_g000067 Rmu_sc0003623.1_g000001 Rmu_sc0003623.1_g000027 Rmu_sc0004622.1_g000009 Rmu_sc0004622.1_g000013 Rmu_sc0004622.1_g000018 Rmu_sc0005229.1_g000007 Rmu_sc0005229.1_g000012 Rmu_sc0006444.1_g000002 Rmu_sc0006444.1_g000006 Rmu_sc0012283.1_g000002 Rmu_sc0012283.1_g000003 Rmu_sc0042918.1_g000001
rosa_roxburghii	Rroxscaffold_2G00117770 Rroxscaffold_5G00346060 Rroxscaffold_5G00346100 Rroxscaffold_5G00346110 Rroxscaffold_5G00346130 Rroxscaffold_5G00360510
rosa_rugosa	Rorug04G0029000 Rorug04G0029200 Rorug04G0029300 Rorug04G0029500 Rorug04G0029600 Rorug04G0030700 Rorug04G0030900
rosa_samantha	Rh4AG107600 Rh4BG101300 Rh4CG115900
rosa_wichuraiana	Rw4G008620 Rw4G008630 Rw4G008650 Rw4G008660 Rw4G008680 Rw4G008710 Rw4G008730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	321
AatII	GACGTC	1 cut(s)	888
AccI	GTMKAC	1 cut(s)	831
AccIII	TCCGGA	1 cut(s)	261
AciI	CCGC	3 cut(s)	407, 634, 1203
AclWI	GGATC	2 cut(s)	523, 611
AcoI	YGGCCR	1 cut(s)	714
AcsI	RAATTY	1 cut(s)	144
AcyI	GRCGYC	1 cut(s)	885
AfaI	GTAC	9 cut(s)	418, 503, 530, 541, 1038, 1043, 1157, 1201, 1264
AfiI	CCNNNNNNNGG	3 cut(s)	756, 922, 1056
AgsI	TTSAA	6 cut(s)	442, 566, 941, 1119, 1185, 1292
AhdI	GACNNNNNGTC	1 cut(s)	1250
AluBI	AGCT	8 cut(s)	53, 159, 259, 571, 701, 837, 961, 1025
AluI	AGCT	8 cut(s)	53, 159, 259, 571, 701, 837, 961, 1025
Alw21I	GWGCWC	1 cut(s)	490
AlwI	GGATC	2 cut(s)	523, 611
Ama87I	CYCGRG	2 cut(s)	916, 1049
Aor13HI	TCCGGA	1 cut(s)	261
AoxI	GGCC	3 cut(s)	295, 714, 1058
ApeKI	GCWGC	2 cut(s)	50, 782
ApoI	RAATTY	1 cut(s)	144
Asp700I	GAANNNNTTC	2 cut(s)	570, 666
AspS9I	GGNCC	2 cut(s)	295, 448
AsuC2I	CCSGG	2 cut(s)	774, 945
AsuHPI	GGTGA	1 cut(s)	85
AvaI	CYCGRG	2 cut(s)	916, 1049
AvaII	GGWCC	1 cut(s)	448
BaeGI	GKGCMC	1 cut(s)	193
BalI	TGGCCA	1 cut(s)	716
Bbv12I	GWGCWC	1 cut(s)	490
BbvCI	CCTCAGC	1 cut(s)	800
BbvI	GCAGC	2 cut(s)	62, 769
BccI	CCATC	2 cut(s)	245, 441
BcnI	CCSGG	2 cut(s)	774, 945
BfaI	CTAG	1 cut(s)	1306
BfmI	CTRYAG	2 cut(s)	60, 832
BisI	GCNGC	2 cut(s)	51, 783
BlsI	GCNGC	2 cut(s)	52, 784
BmcAI	AGTACT	1 cut(s)	530
Bme1390I	CCNGG	2 cut(s)	774, 945
Bme18I	GGWCC	1 cut(s)	448
BmeRI	GACNNNNNGTC	1 cut(s)	1250
BmeT110I	CYCGRG	2 cut(s)	916, 1049
BmgT120I	GGNCC	2 cut(s)	295, 448
BmiI	GGNNCC	2 cut(s)	914, 1224
BmrFI	CCNGG	2 cut(s)	774, 945
BmsI	GCATC	1 cut(s)	667
BplI	GAGNNNNNCTC	2 cut(s)	754, 786
Bpu10I	CCTNAGC	1 cut(s)	800
BpuMI	CCSGG	2 cut(s)	774, 945
BsaBI	GATNNNNATC	2 cut(s)	92, 1070
BsaHI	GRCGYC	1 cut(s)	885
BsaJI	CCNNGG	5 cut(s)	66, 298, 917, 944, 1050
BsaWI	WCCGGW	2 cut(s)	261, 787
Bsc4I	CCNNNNNNNGG	3 cut(s)	756, 922, 1056
Bse1I	ACTGG	2 cut(s)	1206, 1246
Bse3DI	GCAATG	1 cut(s)	343
Bse8I	GATNNNNATC	2 cut(s)	92, 1070
BseAI	TCCGGA	1 cut(s)	261
BseDI	CCNNGG	5 cut(s)	66, 298, 917, 944, 1050
BseGI	GGATG	1 cut(s)	682
BseJI	GATNNNNATC	2 cut(s)	92, 1070
BseLI	CCNNNNNNNGG	3 cut(s)	756, 922, 1056
BseMI	GCAATG	1 cut(s)	343
BseMII	CTCAG	2 cut(s)	496, 791
BseNI	ACTGG	2 cut(s)	1206, 1246
BseRI	GAGGAG	1 cut(s)	998
BseSI	GKGCMC	1 cut(s)	193
BseXI	GCAGC	2 cut(s)	62, 769
BsgI	GTGCAG	4 cut(s)	333, 768, 1116, 1149
BshFI	GGCC	3 cut(s)	297, 716, 1060
BsiHKAI	GWGCWC	1 cut(s)	490
BsiHKCI	CYCGRG	2 cut(s)	916, 1049
BsiSI	CCGG	4 cut(s)	262, 773, 788, 944
BslFI	GGGAC	2 cut(s)	604, 781
BslI	CCNNNNNNNGG	3 cut(s)	756, 922, 1056
BsmFI	GGGAC	2 cut(s)	604, 781
BsmI	GAATGC	2 cut(s)	629, 849
BsnI	GGCC	3 cut(s)	297, 716, 1060
BsoBI	CYCGRG	2 cut(s)	916, 1049
Bsp1286I	GDGCHC	2 cut(s)	193, 490
Bsp13I	TCCGGA	1 cut(s)	261
Bsp1407I	TGTACA	1 cut(s)	1036
Bsp143I	GATC	6 cut(s)	75, 87, 168, 515, 603, 858
Bsp19I	CCATGG	1 cut(s)	66
BspACI	CCGC	3 cut(s)	407, 634, 1203
BspANI	GGCC	3 cut(s)	297, 716, 1060
BspCNI	CTCAG	2 cut(s)	495, 792
BspEI	TCCGGA	1 cut(s)	261
BspHI	TCATGA	2 cut(s)	574, 1272
BspLI	GGNNCC	2 cut(s)	914, 1224
BspPI	GGATC	2 cut(s)	523, 611
BsrDI	GCAATG	1 cut(s)	343
BsrGI	TGTACA	1 cut(s)	1036
BsrI	ACTGG	2 cut(s)	1206, 1246
BssECI	CCNNGG	5 cut(s)	66, 298, 917, 944, 1050
BssMI	GATC	6 cut(s)	75, 87, 168, 515, 603, 858
BssNI	GRCGYC	1 cut(s)	885
BssT1I	CCWWGG	2 cut(s)	66, 298
Bst4CI	ACNGT	4 cut(s)	550, 1041, 1199, 1237
BstACI	GRCGYC	1 cut(s)	885
BstAPI	GCANNNNNTGC	1 cut(s)	56
BstAUI	TGTACA	1 cut(s)	1036
BstDEI	CTNAG	4 cut(s)	79, 160, 482, 800
BstDSI	CCRYGG	1 cut(s)	66
BstF5I	GGATG	1 cut(s)	682
BstKTI	GATC	6 cut(s)	78, 90, 171, 518, 606, 861
BstMBI	GATC	6 cut(s)	75, 87, 168, 515, 603, 858
BstMWI	GCNNNNNNNGC	1 cut(s)	56
BstNSI	RCATGY	1 cut(s)	1048
BstSCI	CCNGG	2 cut(s)	772, 943
BstSFI	CTRYAG	2 cut(s)	60, 832
BstSLI	GKGCMC	1 cut(s)	193
BstV1I	GCAGC	2 cut(s)	62, 769
BstXI	CCANNNNNNTGG	1 cut(s)	550
BsuRI	GGCC	3 cut(s)	297, 716, 1060
BtgI	CCRYGG	1 cut(s)	66
BtsCI	GGATG	1 cut(s)	682
BtsI	GCAGTG	2 cut(s)	111, 360
BtsIMutI	CAGTG	4 cut(s)	111, 360, 1213, 1264
CciI	TCATGA	2 cut(s)	574, 1272
Cfr13I	GGNCC	2 cut(s)	295, 448
CseI	GACGC	1 cut(s)	1240
Csp6I	GTAC	9 cut(s)	417, 502, 529, 540, 1037, 1042, 1156, 1200, 1263
CviAII	CATG	6 cut(s)	67, 505, 575, 751, 1045, 1273
CviQI	GTAC	9 cut(s)	417, 502, 529, 540, 1037, 1042, 1156, 1200, 1263
DdeI	CTNAG	4 cut(s)	79, 160, 482, 800
DpnI	GATC	6 cut(s)	77, 89, 170, 517, 605, 860
DpnII	GATC	6 cut(s)	75, 87, 168, 515, 603, 858
DriI	GACNNNNNGTC	1 cut(s)	1250
EaeI	YGGCCR	1 cut(s)	714
Eam1105I	GACNNNNNGTC	1 cut(s)	1250
EciI	GGCGGA	1 cut(s)	422
Eco130I	CCWWGG	2 cut(s)	66, 298
Eco147I	AGGCCT	1 cut(s)	1060
Eco47I	GGWCC	1 cut(s)	448
Eco88I	CYCGRG	2 cut(s)	916, 1049
EcoRI	GAATTC	1 cut(s)	144
EcoT14I	CCWWGG	2 cut(s)	66, 298
ErhI	CCWWGG	2 cut(s)	66, 298
FaeI	CATG	6 cut(s)	70, 508, 578, 754, 1048, 1276
FaqI	GGGAC	2 cut(s)	604, 781
FatI	CATG	6 cut(s)	66, 504, 574, 750, 1044, 1272
FblI	GTMKAC	1 cut(s)	831
Fnu4HI	GCNGC	2 cut(s)	51, 783
FokI	GGATG	1 cut(s)	689
Fsp4HI	GCNGC	2 cut(s)	51, 783
FspBI	CTAG	1 cut(s)	1306
GluI	GCNGC	2 cut(s)	51, 783
HaeIII	GGCC	3 cut(s)	297, 716, 1060
HapII	CCGG	4 cut(s)	262, 773, 788, 944
HgaI	GACGC	1 cut(s)	1240
Hin1I	GRCGYC	1 cut(s)	885
Hin1II	CATG	6 cut(s)	70, 508, 578, 754, 1048, 1276
HindIII	AAGCTT	1 cut(s)	569
HinfI	GANTC	6 cut(s)	7, 14, 387, 769, 877, 1105
HpaII	CCGG	4 cut(s)	262, 773, 788, 944
HphI	GGTGA	1 cut(s)	85
Hpy166II	GTNNAC	4 cut(s)	246, 496, 832, 1037
Hpy188I	TCNGA	6 cut(s)	114, 150, 379, 425, 485, 1147
Hpy8I	GTNNAC	4 cut(s)	246, 496, 832, 1037
Hpy99I	CGWCG	2 cut(s)	887, 1256
HpyAV	CCTTC	5 cut(s)	352, 861, 867, 1085, 1125
HpyCH4III	ACNGT	4 cut(s)	550, 1041, 1199, 1237
HpyCH4IV	ACGT	1 cut(s)	885
HpyCH4V	TGCA	7 cut(s)	104, 314, 616, 680, 785, 1097, 1166
HpyF10VI	GCNNNNNNNGC	1 cut(s)	56
HpyF3I	CTNAG	4 cut(s)	79, 160, 482, 800
HpySE526I	ACGT	1 cut(s)	885
Hsp92I	GRCGYC	1 cut(s)	885
Hsp92II	CATG	6 cut(s)	70, 508, 578, 754, 1048, 1276
Kpn2I	TCCGGA	1 cut(s)	261
Kzo9I	GATC	6 cut(s)	75, 87, 168, 515, 603, 858
LmnI	GCTCC	1 cut(s)	264
Lsp1109I	GCAGC	2 cut(s)	62, 769
LweI	GCATC	1 cut(s)	667
MaeI	CTAG	1 cut(s)	1306
MaeII	ACGT	1 cut(s)	885
MaeIII	GTNAC	2 cut(s)	725, 1237
MalI	GATC	6 cut(s)	77, 89, 170, 517, 605, 860
MboI	GATC	6 cut(s)	75, 87, 168, 515, 603, 858
MboII	GAAGA	5 cut(s)	178, 438, 632, 943, 1288
MfeI	CAATTG	1 cut(s)	99
MhlI	GDGCHC	2 cut(s)	193, 490
MlsI	TGGCCA	1 cut(s)	716
MluCI	AATT	4 cut(s)	99, 144, 398, 950
MluNI	TGGCCA	1 cut(s)	716
MlyI	GAGTC	2 cut(s)	16, 763
MmeI	TCCRAC	3 cut(s)	1068, 1125, 1214
Mox20I	TGGCCA	1 cut(s)	716
MroI	TCCGGA	1 cut(s)	261
MroXI	GAANNNNTTC	2 cut(s)	570, 666
MscI	TGGCCA	1 cut(s)	716
MseI	TTAA	1 cut(s)	1080
MslI	CAYNNNNRTG	3 cut(s)	71, 820, 822
Msp20I	TGGCCA	1 cut(s)	716
MspA1I	CMGCKG	1 cut(s)	634
MspI	CCGG	4 cut(s)	262, 773, 788, 944
MspR9I	CCNGG	2 cut(s)	774, 945
MunI	CAATTG	1 cut(s)	99
Mva1269I	GAATGC	2 cut(s)	629, 849
MwoI	GCNNNNNNNGC	1 cut(s)	56
NciI	CCSGG	2 cut(s)	774, 945
NcoI	CCATGG	1 cut(s)	66
NdeII	GATC	6 cut(s)	75, 87, 168, 515, 603, 858
NlaIII	CATG	6 cut(s)	70, 508, 578, 754, 1048, 1276
NlaIV	GGNNCC	2 cut(s)	914, 1224
NspI	RCATGY	1 cut(s)	1048
PagI	TCATGA	2 cut(s)	574, 1272
PceI	AGGCCT	1 cut(s)	1060
PcsI	WCGNNNNNNNCGW	1 cut(s)	1151
PctI	GAATGC	2 cut(s)	629, 849
PdmI	GAANNNNTTC	2 cut(s)	570, 666
PfeI	GAWTC	4 cut(s)	14, 387, 877, 1105
PkrI	GCNGC	2 cut(s)	52, 784
PleI	GAGTC	2 cut(s)	15, 763
PpsI	GAGTC	2 cut(s)	15, 763
PsiI	TTATAA	1 cut(s)	321
PspN4I	GGNNCC	2 cut(s)	914, 1224
PspPI	GGNCC	2 cut(s)	295, 448
RsaI	GTAC	9 cut(s)	418, 503, 530, 541, 1038, 1043, 1157, 1201, 1264
RsaNI	GTAC	9 cut(s)	417, 502, 529, 540, 1037, 1042, 1156, 1200, 1263
RseI	CAYNNNNRTG	3 cut(s)	71, 820, 822
SaqAI	TTAA	1 cut(s)	1080
SatI	GCNGC	2 cut(s)	51, 783
Sau3AI	GATC	6 cut(s)	75, 87, 168, 515, 603, 858
Sau96I	GGNCC	2 cut(s)	295, 448
ScaI	AGTACT	1 cut(s)	530
SchI	GAGTC	2 cut(s)	16, 763
ScrFI	CCNGG	2 cut(s)	774, 945
SduI	GDGCHC	2 cut(s)	193, 490
SfaNI	GCATC	1 cut(s)	667
SfcI	CTRYAG	2 cut(s)	60, 832
SinI	GGWCC	1 cut(s)	448
SmiMI	CAYNNNNRTG	3 cut(s)	71, 820, 822
Sse9I	AATT	4 cut(s)	99, 144, 398, 950
SseBI	AGGCCT	1 cut(s)	1060
SsiI	CCGC	3 cut(s)	407, 634, 1203
SspI	AATATT	2 cut(s)	330, 667
SspMI	CTAG	1 cut(s)	1306
StuI	AGGCCT	1 cut(s)	1060
StyD4I	CCNGG	2 cut(s)	772, 943
StyI	CCWWGG	2 cut(s)	66, 298
TaaI	ACNGT	4 cut(s)	550, 1041, 1199, 1237
TaiI	ACGT	1 cut(s)	888
TaqI	TCGA	4 cut(s)	5, 471, 855, 882
TasI	AATT	4 cut(s)	99, 144, 398, 950
TatI	WGTACW	5 cut(s)	416, 501, 528, 1036, 1041
TfiI	GAWTC	4 cut(s)	14, 387, 877, 1105
Tru1I	TTAA	1 cut(s)	1080
Tru9I	TTAA	1 cut(s)	1080
TscAI	CASTG	4 cut(s)	111, 360, 1213, 1264
TseI	GCWGC	2 cut(s)	50, 782
TspDTI	ATGAA	3 cut(s)	6, 563, 1289
TspGWI	ACGGA	2 cut(s)	440, 1143
TspRI	CASTG	4 cut(s)	111, 360, 1213, 1264
VpaK11BI	GGWCC	1 cut(s)	448
XapI	RAATTY	1 cut(s)	144
XceI	RCATGY	1 cut(s)	1048
XcmI	CCANNNNNNNNNTGG	1 cut(s)	305
XmiI	GTMKAC	1 cut(s)	831
XmnI	GAANNNNTTC	2 cut(s)	570, 666
XspI	CTAG	1 cut(s)	1306
ZraI	GACGTC	1 cut(s)	886
ZrmI	AGTACT	1 cut(s)	530

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.