Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 117 |
| AccI | GTMKAC | 1 cut(s) | 1091 |
| AciI | CCGC | 4 cut(s) | 203, 377, 595, 768 |
| AclWI | GGATC | 1 cut(s) | 1007 |
| AcsI | RAATTY | 2 cut(s) | 225, 475 |
| AcuI | CTGAAG | 2 cut(s) | 41, 603 |
| AfaI | GTAC | 3 cut(s) | 214, 299, 1047 |
| AfeI | AGCGCT | 1 cut(s) | 1000 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 549, 715 |
| AgsI | TTSAA | 6 cut(s) | 238, 359, 689, 912, 978, 1082 |
| AjnI | CCWGG | 2 cut(s) | 565, 736 |
| AleI | CACNNNNGTG | 1 cut(s) | 615 |
| AluBI | AGCT | 6 cut(s) | 171, 364, 494, 514, 630, 890 |
| AluI | AGCT | 6 cut(s) | 171, 364, 494, 514, 630, 890 |
| Alw21I | GWGCWC | 2 cut(s) | 173, 286 |
| AlwI | GGATC | 1 cut(s) | 1007 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1031 |
| Aor51HI | AGCGCT | 1 cut(s) | 1000 |
| AoxI | GGCC | 1 cut(s) | 851 |
| ApeKI | GCWGC | 1 cut(s) | 575 |
| ApoI | RAATTY | 2 cut(s) | 225, 475 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 338, 370 |
| AseI | ATTAAT | 1 cut(s) | 681 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 363, 459 |
| AspLEI | GCGC | 1 cut(s) | 1001 |
| AsuHPI | GGTGA | 2 cut(s) | 70, 883 |
| AxyI | CCTNAGG | 1 cut(s) | 843 |
| BanII | GRGCYC | 1 cut(s) | 173 |
| BbsI | GAAGAC | 1 cut(s) | 242 |
| Bbv12I | GWGCWC | 2 cut(s) | 173, 286 |
| BbvI | GCAGC | 1 cut(s) | 562 |
| BccI | CCATC | 1 cut(s) | 41 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 837, 871 |
| BciT130I | CCWGG | 2 cut(s) | 567, 738 |
| BciVI | GTATCC | 2 cut(s) | 934, 1028 |
| BfaI | CTAG | 5 cut(s) | 242, 746, 903, 996, 1096 |
| BfoI | RGCGCY | 1 cut(s) | 1002 |
| BfuI | GTATCC | 2 cut(s) | 934, 1028 |
| BisI | GCNGC | 1 cut(s) | 576 |
| BlpI | GCTNAGC | 1 cut(s) | 510 |
| BlsI | GCNGC | 1 cut(s) | 577 |
| Bme1390I | CCNGG | 2 cut(s) | 567, 738 |
| BmiI | GGNNCC | 2 cut(s) | 139, 707 |
| BmrFI | CCNGG | 2 cut(s) | 567, 738 |
| BmrI | ACTGGG | 1 cut(s) | 558 |
| BmsI | GCATC | 2 cut(s) | 582, 627 |
| BmuI | ACTGGG | 1 cut(s) | 558 |
| BpiI | GAAGAC | 1 cut(s) | 242 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 547, 579 |
| BpmI | CTGGAG | 1 cut(s) | 759 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 510 |
| BsaJI | CCNNGG | 1 cut(s) | 710 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 549, 715 |
| Bse1I | ACTGG | 4 cut(s) | 553, 707, 832, 1036 |
| Bse21I | CCTNAGG | 1 cut(s) | 843 |
| BseBI | CCWGG | 2 cut(s) | 567, 738 |
| BseDI | CCNNGG | 1 cut(s) | 710 |
| BseGI | GGATG | 3 cut(s) | 229, 597, 642 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 549, 715 |
| BseMII | CTCAG | 4 cut(s) | 186, 292, 789, 834 |
| BseNI | ACTGG | 4 cut(s) | 553, 707, 832, 1036 |
| BseRI | GAGGAG | 1 cut(s) | 791 |
| BseXI | GCAGC | 1 cut(s) | 562 |
| BsgI | GTGCAG | 3 cut(s) | 129, 561, 942 |
| BshFI | GGCC | 1 cut(s) | 853 |
| BsiHKAI | GWGCWC | 2 cut(s) | 173, 286 |
| BslFI | GGGAC | 1 cut(s) | 397 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 549, 715 |
| BsmFI | GGGAC | 1 cut(s) | 397 |
| BsmI | GAATGC | 1 cut(s) | 422 |
| BsnI | GGCC | 1 cut(s) | 853 |
| Bsp1286I | GDGCHC | 2 cut(s) | 173, 286 |
| Bsp1407I | TGTACA | 1 cut(s) | 1045 |
| Bsp143I | GATC | 2 cut(s) | 651, 1012 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 510 |
| BspACI | CCGC | 4 cut(s) | 203, 377, 595, 768 |
| BspANI | GGCC | 1 cut(s) | 853 |
| BspCNI | CTCAG | 4 cut(s) | 185, 291, 790, 835 |
| BspHI | TCATGA | 2 cut(s) | 367, 1062 |
| BspLI | GGNNCC | 2 cut(s) | 139, 707 |
| BspPI | GGATC | 1 cut(s) | 1007 |
| BsrGI | TGTACA | 1 cut(s) | 1045 |
| BsrI | ACTGG | 4 cut(s) | 553, 707, 832, 1036 |
| BssECI | CCNNGG | 1 cut(s) | 710 |
| BssMI | GATC | 2 cut(s) | 651, 1012 |
| BssT1I | CCWWGG | 1 cut(s) | 710 |
| Bst2UI | CCWGG | 2 cut(s) | 567, 738 |
| Bst4CI | ACNGT | 1 cut(s) | 1027 |
| BstAUI | TGTACA | 1 cut(s) | 1045 |
| BstDEI | CTNAG | 6 cut(s) | 172, 278, 349, 510, 798, 843 |
| BstF5I | GGATG | 3 cut(s) | 229, 597, 642 |
| BstH2I | RGCGCY | 1 cut(s) | 1002 |
| BstHHI | GCGC | 1 cut(s) | 1001 |
| BstKTI | GATC | 2 cut(s) | 654, 1015 |
| BstMBI | GATC | 2 cut(s) | 651, 1012 |
| BstNI | CCWGG | 2 cut(s) | 567, 738 |
| BstNSI | RCATGY | 1 cut(s) | 304 |
| BstSCI | CCNGG | 2 cut(s) | 565, 736 |
| BstV1I | GCAGC | 1 cut(s) | 562 |
| BstV2I | GAAGAC | 1 cut(s) | 242 |
| BstX2I | RGATCY | 1 cut(s) | 1012 |
| BstYI | RGATCY | 1 cut(s) | 1012 |
| Bsu36I | CCTNAGG | 1 cut(s) | 843 |
| BsuI | GTATCC | 2 cut(s) | 934, 1028 |
| BsuRI | GGCC | 1 cut(s) | 853 |
| BtsCI | GGATG | 3 cut(s) | 229, 597, 642 |
| BtsI | GCAGTG | 1 cut(s) | 156 |
| BtsIMutI | CAGTG | 1 cut(s) | 156 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1031 |
| CciI | TCATGA | 2 cut(s) | 367, 1062 |
| CfoI | GCGC | 1 cut(s) | 1001 |
| CseI | GACGC | 2 cut(s) | 479, 1077 |
| Csp6I | GTAC | 3 cut(s) | 213, 298, 1046 |
| CviAII | CATG | 7 cut(s) | 254, 301, 368, 410, 544, 1049, 1063 |
| CviQI | GTAC | 3 cut(s) | 213, 298, 1046 |
| DdeI | CTNAG | 6 cut(s) | 172, 278, 349, 510, 798, 843 |
| DpnI | GATC | 2 cut(s) | 653, 1014 |
| DpnII | GATC | 2 cut(s) | 651, 1012 |
| EciI | GGCGGA | 1 cut(s) | 218 |
| Ecl136II | GAGCTC | 1 cut(s) | 171 |
| Eco130I | CCWWGG | 1 cut(s) | 710 |
| Eco147I | AGGCCT | 1 cut(s) | 853 |
| Eco24I | GRGCYC | 1 cut(s) | 173 |
| Eco47III | AGCGCT | 1 cut(s) | 1000 |
| Eco53kI | GAGCTC | 1 cut(s) | 171 |
| Eco57I | CTGAAG | 2 cut(s) | 41, 603 |
| Eco81I | CCTNAGG | 1 cut(s) | 843 |
| EcoICRI | GAGCTC | 1 cut(s) | 171 |
| EcoRI | GAATTC | 1 cut(s) | 225 |
| EcoRII | CCWGG | 2 cut(s) | 565, 736 |
| EcoT14I | CCWWGG | 1 cut(s) | 710 |
| EcoT38I | GRGCYC | 1 cut(s) | 173 |
| ErhI | CCWWGG | 1 cut(s) | 710 |
| FaeI | CATG | 7 cut(s) | 257, 304, 371, 413, 547, 1052, 1066 |
| FaqI | GGGAC | 1 cut(s) | 397 |
| FatI | CATG | 7 cut(s) | 253, 300, 367, 409, 543, 1048, 1062 |
| FblI | GTMKAC | 1 cut(s) | 1091 |
| Fnu4HI | GCNGC | 1 cut(s) | 576 |
| FokI | GGATG | 3 cut(s) | 216, 604, 649 |
| FriOI | GRGCYC | 1 cut(s) | 173 |
| Fsp4HI | GCNGC | 1 cut(s) | 576 |
| FspBI | CTAG | 5 cut(s) | 242, 746, 903, 996, 1096 |
| GlaI | GCGC | 1 cut(s) | 1000 |
| GluI | GCNGC | 1 cut(s) | 576 |
| GsuI | CTGGAG | 1 cut(s) | 759 |
| HaeII | RGCGCY | 1 cut(s) | 1002 |
| HaeIII | GGCC | 1 cut(s) | 853 |
| HgaI | GACGC | 2 cut(s) | 479, 1077 |
| HhaI | GCGC | 1 cut(s) | 1001 |
| Hin1II | CATG | 7 cut(s) | 257, 304, 371, 413, 547, 1052, 1066 |
| Hin6I | GCGC | 1 cut(s) | 999 |
| HinP1I | GCGC | 1 cut(s) | 999 |
| HindIII | AAGCTT | 1 cut(s) | 362 |
| HinfI | GANTC | 3 cut(s) | 4, 670, 898 |
| HphI | GGTGA | 2 cut(s) | 70, 883 |
| Hpy166II | GTNNAC | 3 cut(s) | 42, 625, 1092 |
| Hpy188I | TCNGA | 5 cut(s) | 60, 175, 281, 940, 1012 |
| Hpy188III | TCNNGA | 5 cut(s) | 368, 674, 689, 1020, 1063 |
| Hpy8I | GTNNAC | 3 cut(s) | 42, 625, 1092 |
| HpyAV | CCTTC | 5 cut(s) | 148, 660, 864, 878, 918 |
| HpyCH4III | ACNGT | 1 cut(s) | 1027 |
| HpyCH4IV | ACGT | 1 cut(s) | 38 |
| HpyCH4V | TGCA | 5 cut(s) | 65, 110, 409, 578, 959 |
| HpyF3I | CTNAG | 6 cut(s) | 172, 278, 349, 510, 798, 843 |
| HpySE526I | ACGT | 1 cut(s) | 38 |
| Hsp92II | CATG | 7 cut(s) | 257, 304, 371, 413, 547, 1052, 1066 |
| HspAI | GCGC | 1 cut(s) | 999 |
| Kzo9I | GATC | 2 cut(s) | 651, 1012 |
| LmnI | GCTCC | 2 cut(s) | 168, 887 |
| Lsp1109I | GCAGC | 1 cut(s) | 562 |
| LweI | GCATC | 2 cut(s) | 582, 627 |
| MaeI | CTAG | 5 cut(s) | 242, 746, 903, 996, 1096 |
| MaeII | ACGT | 1 cut(s) | 38 |
| MaeIII | GTNAC | 2 cut(s) | 518, 1027 |
| MalI | GATC | 2 cut(s) | 653, 1014 |
| MboI | GATC | 2 cut(s) | 651, 1012 |
| MboII | GAAGA | 4 cut(s) | 234, 242, 425, 736 |
| MfeI | CAATTG | 1 cut(s) | 216 |
| MflI | RGATCY | 1 cut(s) | 1012 |
| MhlI | GDGCHC | 2 cut(s) | 173, 286 |
| MluCI | AATT | 6 cut(s) | 194, 216, 225, 475, 804, 1077 |
| MmeI | TCCRAC | 2 cut(s) | 861, 918 |
| MroXI | GAANNNNTTC | 2 cut(s) | 363, 459 |
| MseI | TTAA | 1 cut(s) | 681 |
| MslI | CAYNNNNRTG | 2 cut(s) | 613, 615 |
| MspR9I | CCNGG | 2 cut(s) | 567, 738 |
| MunI | CAATTG | 1 cut(s) | 216 |
| Mva1269I | GAATGC | 1 cut(s) | 422 |
| MvaI | CCWGG | 2 cut(s) | 567, 738 |
| NdeII | GATC | 2 cut(s) | 651, 1012 |
| NlaIII | CATG | 7 cut(s) | 257, 304, 371, 413, 547, 1052, 1066 |
| NlaIV | GGNNCC | 2 cut(s) | 139, 707 |
| NspI | RCATGY | 1 cut(s) | 304 |
| OliI | CACNNNNGTG | 1 cut(s) | 615 |
| PagI | TCATGA | 2 cut(s) | 367, 1062 |
| PceI | AGGCCT | 1 cut(s) | 853 |
| PctI | GAATGC | 1 cut(s) | 422 |
| PdmI | GAANNNNTTC | 2 cut(s) | 363, 459 |
| PfeI | GAWTC | 3 cut(s) | 4, 670, 898 |
| PkrI | GCNGC | 1 cut(s) | 577 |
| PshBI | ATTAAT | 1 cut(s) | 681 |
| PsiI | TTATAA | 1 cut(s) | 117 |
| Psp124BI | GAGCTC | 1 cut(s) | 173 |
| Psp6I | CCWGG | 2 cut(s) | 565, 736 |
| PspGI | CCWGG | 2 cut(s) | 565, 736 |
| PspN4I | GGNNCC | 2 cut(s) | 139, 707 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1031 |
| PsuI | RGATCY | 1 cut(s) | 1012 |
| RsaI | GTAC | 3 cut(s) | 214, 299, 1047 |
| RsaNI | GTAC | 3 cut(s) | 213, 298, 1046 |
| RseI | CAYNNNNRTG | 2 cut(s) | 613, 615 |
| SacI | GAGCTC | 1 cut(s) | 173 |
| SaqAI | TTAA | 1 cut(s) | 681 |
| SatI | GCNGC | 1 cut(s) | 576 |
| Sau3AI | GATC | 2 cut(s) | 651, 1012 |
| ScrFI | CCNGG | 2 cut(s) | 567, 738 |
| SduI | GDGCHC | 2 cut(s) | 173, 286 |
| SfaNI | GCATC | 2 cut(s) | 582, 627 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 613, 615 |
| Sse9I | AATT | 6 cut(s) | 194, 216, 225, 475, 804, 1077 |
| SseBI | AGGCCT | 1 cut(s) | 853 |
| SsiI | CCGC | 4 cut(s) | 203, 377, 595, 768 |
| SspI | AATATT | 2 cut(s) | 460, 685 |
| SspMI | CTAG | 5 cut(s) | 242, 746, 903, 996, 1096 |
| SstI | GAGCTC | 1 cut(s) | 173 |
| StuI | AGGCCT | 1 cut(s) | 853 |
| StyD4I | CCNGG | 2 cut(s) | 565, 736 |
| StyI | CCWWGG | 1 cut(s) | 710 |
| TaaI | ACNGT | 1 cut(s) | 1027 |
| TaiI | ACGT | 1 cut(s) | 41 |
| TaqI | TCGA | 1 cut(s) | 857 |
| TasI | AATT | 6 cut(s) | 194, 216, 225, 475, 804, 1077 |
| TatI | WGTACW | 2 cut(s) | 297, 1045 |
| TfiI | GAWTC | 3 cut(s) | 4, 670, 898 |
| Tru1I | TTAA | 1 cut(s) | 681 |
| Tru9I | TTAA | 1 cut(s) | 681 |
| TscAI | CASTG | 1 cut(s) | 156 |
| TseI | GCWGC | 1 cut(s) | 575 |
| TspDTI | ATGAA | 7 cut(s) | 218, 242, 323, 356, 384, 426, 1089 |
| TspGWI | ACGGA | 1 cut(s) | 236 |
| TspRI | CASTG | 1 cut(s) | 156 |
| VspI | ATTAAT | 1 cut(s) | 681 |
| XapI | RAATTY | 2 cut(s) | 225, 475 |
| XceI | RCATGY | 1 cut(s) | 304 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 717 |
| XmiI | GTMKAC | 1 cut(s) | 1091 |
| XmnI | GAANNNNTTC | 2 cut(s) | 363, 459 |
| XspI | CTAG | 5 cut(s) | 242, 746, 903, 996, 1096 |