Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 267 |
| Acc65I | GGTACC | 1 cut(s) | 317 |
| AccB1I | GGYRCC | 1 cut(s) | 317 |
| AccI | GTMKAC | 2 cut(s) | 69, 825 |
| AccIII | TCCGGA | 1 cut(s) | 838 |
| AciI | CCGC | 3 cut(s) | 353, 527, 947 |
| AclWI | GGATC | 2 cut(s) | 101, 116 |
| AcoI | YGGCCR | 1 cut(s) | 657 |
| AcsI | RAATTY | 1 cut(s) | 275 |
| AcuI | CTGAAG | 1 cut(s) | 191 |
| AfaI | GTAC | 3 cut(s) | 259, 319, 776 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 372, 866, 999 |
| AgsI | TTSAA | 5 cut(s) | 338, 670, 884, 1062, 1253 |
| AhlI | ACTAGT | 3 cut(s) | 1118, 1166, 1208 |
| AjnI | CCWGG | 1 cut(s) | 715 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 513, 545 |
| AleI | CACNNNNGTG | 1 cut(s) | 765 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1033, 1065 |
| AluBI | AGCT | 8 cut(s) | 99, 514, 539, 644, 664, 780, 904, 968 |
| AluI | AGCT | 8 cut(s) | 99, 514, 539, 644, 664, 780, 904, 968 |
| Alw26I | GTCTC | 3 cut(s) | 118, 415, 843 |
| AlwI | GGATC | 2 cut(s) | 101, 116 |
| Ama87I | CYCGRG | 1 cut(s) | 992 |
| Aor13HI | TCCGGA | 1 cut(s) | 838 |
| AoxI | GGCC | 3 cut(s) | 657, 989, 1001 |
| ApeKI | GCWGC | 5 cut(s) | 96, 574, 725, 742, 944 |
| ApoI | RAATTY | 1 cut(s) | 275 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 513, 609 |
| Asp718I | GGTACC | 1 cut(s) | 317 |
| AspS9I | GGNCC | 2 cut(s) | 307, 990 |
| AsuHPI | GGTGA | 2 cut(s) | 220, 1033 |
| AsuII | TTCGAA | 1 cut(s) | 507 |
| AvaI | CYCGRG | 1 cut(s) | 992 |
| AvaII | GGWCC | 1 cut(s) | 307 |
| BalI | TGGCCA | 1 cut(s) | 659 |
| BanI | GGYRCC | 1 cut(s) | 317 |
| BbsI | GAAGAC | 2 cut(s) | 1117, 1207 |
| BbvI | GCAGC | 5 cut(s) | 108, 586, 712, 729, 956 |
| BccI | CCATC | 2 cut(s) | 191, 864 |
| BciT130I | CCWGG | 1 cut(s) | 717 |
| BcoDI | GTCTC | 3 cut(s) | 118, 415, 843 |
| BcuI | ACTAGT | 3 cut(s) | 1118, 1166, 1208 |
| BfaI | CTAG | 6 cut(s) | 896, 911, 1053, 1119, 1167, 1209 |
| BfmI | CTRYAG | 1 cut(s) | 743 |
| BisI | GCNGC | 5 cut(s) | 97, 575, 726, 743, 945 |
| BlsI | GCNGC | 5 cut(s) | 98, 576, 727, 744, 946 |
| Bme1390I | CCNGG | 1 cut(s) | 717 |
| Bme18I | GGWCC | 1 cut(s) | 307 |
| BmeT110I | CYCGRG | 1 cut(s) | 992 |
| BmgT120I | GGNCC | 2 cut(s) | 307, 990 |
| BmiI | GGNNCC | 2 cut(s) | 289, 319 |
| BmrFI | CCNGG | 1 cut(s) | 717 |
| BmrI | ACTGGG | 1 cut(s) | 1064 |
| BmsI | GCATC | 1 cut(s) | 1115 |
| BmuI | ACTGGG | 1 cut(s) | 1064 |
| BpiI | GAAGAC | 2 cut(s) | 1117, 1207 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 697, 729 |
| Bpu14I | TTCGAA | 1 cut(s) | 507 |
| BsaBI | GATNNNNATC | 1 cut(s) | 56 |
| BsaJI | CCNNGG | 4 cut(s) | 321, 367, 484, 860 |
| BsaWI | WCCGGW | 1 cut(s) | 838 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 941, 971 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 372, 866, 999 |
| Bse1I | ACTGG | 3 cut(s) | 110, 857, 1070 |
| Bse8I | GATNNNNATC | 1 cut(s) | 56 |
| BseAI | TCCGGA | 1 cut(s) | 838 |
| BseBI | CCWGG | 1 cut(s) | 717 |
| BseDI | CCNNGG | 4 cut(s) | 321, 367, 484, 860 |
| BseGI | GGATG | 3 cut(s) | 625, 875, 1262 |
| BseJI | GATNNNNATC | 1 cut(s) | 56 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 372, 866, 999 |
| BseMII | CTCAG | 2 cut(s) | 442, 1035 |
| BseNI | ACTGG | 3 cut(s) | 110, 857, 1070 |
| BseRI | GAGGAG | 1 cut(s) | 941 |
| BseXI | GCAGC | 5 cut(s) | 108, 586, 712, 729, 956 |
| BseYI | CCCAGC | 1 cut(s) | 238 |
| BsgI | GTGCAG | 1 cut(s) | 711 |
| BshFI | GGCC | 3 cut(s) | 659, 991, 1003 |
| BshNI | GGYRCC | 1 cut(s) | 317 |
| BsiHKCI | CYCGRG | 1 cut(s) | 992 |
| BsiSI | CCGG | 2 cut(s) | 839, 888 |
| BslFI | GGGAC | 1 cut(s) | 547 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 372, 866, 999 |
| BsmAI | GTCTC | 3 cut(s) | 118, 415, 843 |
| BsmFI | GGGAC | 1 cut(s) | 547 |
| BsmI | GAATGC | 2 cut(s) | 572, 792 |
| BsnI | GGCC | 3 cut(s) | 659, 991, 1003 |
| BsoBI | CYCGRG | 1 cut(s) | 992 |
| Bsp119I | TTCGAA | 1 cut(s) | 507 |
| Bsp13I | TCCGGA | 1 cut(s) | 838 |
| Bsp1407I | TGTACA | 1 cut(s) | 774 |
| Bsp143I | GATC | 3 cut(s) | 106, 121, 801 |
| Bsp19I | CCATGG | 1 cut(s) | 484 |
| BspACI | CCGC | 3 cut(s) | 353, 527, 947 |
| BspANI | GGCC | 3 cut(s) | 659, 991, 1003 |
| BspCNI | CTCAG | 2 cut(s) | 441, 1036 |
| BspEI | TCCGGA | 1 cut(s) | 838 |
| BspHI | TCATGA | 2 cut(s) | 118, 517 |
| BspLI | GGNNCC | 2 cut(s) | 289, 319 |
| BspMAI | CTGCAG | 1 cut(s) | 747 |
| BspPI | GGATC | 2 cut(s) | 101, 116 |
| BspT104I | TTCGAA | 1 cut(s) | 507 |
| BspT107I | GGYRCC | 1 cut(s) | 317 |
| BsrGI | TGTACA | 1 cut(s) | 774 |
| BsrI | ACTGG | 3 cut(s) | 110, 857, 1070 |
| BssECI | CCNNGG | 4 cut(s) | 321, 367, 484, 860 |
| BssMI | GATC | 3 cut(s) | 106, 121, 801 |
| BssNAI | GTATAC | 1 cut(s) | 70 |
| BssT1I | CCWWGG | 3 cut(s) | 367, 484, 860 |
| Bst1107I | GTATAC | 1 cut(s) | 70 |
| Bst2UI | CCWGG | 1 cut(s) | 717 |
| Bst4CI | ACNGT | 2 cut(s) | 22, 262 |
| BstAUI | TGTACA | 1 cut(s) | 774 |
| BstBI | TTCGAA | 1 cut(s) | 507 |
| BstC8I | GCNNGC | 1 cut(s) | 572 |
| BstDEI | CTNAG | 2 cut(s) | 428, 1044 |
| BstDSI | CCRYGG | 1 cut(s) | 484 |
| BstF5I | GGATG | 3 cut(s) | 625, 875, 1262 |
| BstKTI | GATC | 3 cut(s) | 109, 124, 804 |
| BstMAI | GTCTC | 3 cut(s) | 118, 415, 843 |
| BstMBI | GATC | 3 cut(s) | 106, 121, 801 |
| BstNI | CCWGG | 1 cut(s) | 717 |
| BstSCI | CCNGG | 1 cut(s) | 715 |
| BstSFI | CTRYAG | 1 cut(s) | 743 |
| BstV1I | GCAGC | 5 cut(s) | 108, 586, 712, 729, 956 |
| BstV2I | GAAGAC | 2 cut(s) | 1117, 1207 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 238, 738 |
| BstZ17I | GTATAC | 1 cut(s) | 70 |
| BsuRI | GGCC | 3 cut(s) | 659, 991, 1003 |
| BtgI | CCRYGG | 1 cut(s) | 484 |
| BtsCI | GGATG | 3 cut(s) | 625, 875, 1262 |
| BtsI | GCAGTG | 2 cut(s) | 306, 752 |
| BtsIMutI | CAGTG | 2 cut(s) | 306, 752 |
| Cac8I | GCNNGC | 1 cut(s) | 572 |
| CciI | TCATGA | 2 cut(s) | 118, 517 |
| Cfr13I | GGNCC | 2 cut(s) | 307, 990 |
| Csp6I | GTAC | 3 cut(s) | 258, 318, 775 |
| CviAII | CATG | 6 cut(s) | 119, 349, 485, 518, 1102, 1222 |
| CviQI | GTAC | 3 cut(s) | 258, 318, 775 |
| DdeI | CTNAG | 2 cut(s) | 428, 1044 |
| DpnI | GATC | 3 cut(s) | 108, 123, 803 |
| DpnII | GATC | 3 cut(s) | 106, 121, 801 |
| EaeI | YGGCCR | 1 cut(s) | 657 |
| EciI | GGCGGA | 1 cut(s) | 368 |
| Eco130I | CCWWGG | 3 cut(s) | 367, 484, 860 |
| Eco147I | AGGCCT | 1 cut(s) | 1003 |
| Eco47I | GGWCC | 1 cut(s) | 307 |
| Eco57I | CTGAAG | 1 cut(s) | 191 |
| Eco88I | CYCGRG | 1 cut(s) | 992 |
| EcoO109I | RGGNCCY | 1 cut(s) | 307 |
| EcoRII | CCWGG | 1 cut(s) | 715 |
| EcoT14I | CCWWGG | 3 cut(s) | 367, 484, 860 |
| ErhI | CCWWGG | 3 cut(s) | 367, 484, 860 |
| FaeI | CATG | 6 cut(s) | 122, 352, 488, 521, 1105, 1225 |
| FaqI | GGGAC | 1 cut(s) | 547 |
| FatI | CATG | 6 cut(s) | 118, 348, 484, 517, 1101, 1221 |
| FblI | GTMKAC | 2 cut(s) | 69, 825 |
| Fnu4HI | GCNGC | 5 cut(s) | 97, 575, 726, 743, 945 |
| FokI | GGATG | 2 cut(s) | 632, 882 |
| Fsp4HI | GCNGC | 5 cut(s) | 97, 575, 726, 743, 945 |
| FspBI | CTAG | 6 cut(s) | 896, 911, 1053, 1119, 1167, 1209 |
| GluI | GCNGC | 5 cut(s) | 97, 575, 726, 743, 945 |
| GsaI | CCCAGC | 1 cut(s) | 242 |
| HaeIII | GGCC | 3 cut(s) | 659, 991, 1003 |
| HapII | CCGG | 2 cut(s) | 839, 888 |
| Hin1II | CATG | 6 cut(s) | 122, 352, 488, 521, 1105, 1225 |
| HindIII | AAGCTT | 1 cut(s) | 512 |
| HinfI | GANTC | 8 cut(s) | 376, 504, 682, 844, 1048, 1136, 1178, 1226 |
| HpaII | CCGG | 2 cut(s) | 839, 888 |
| HphI | GGTGA | 2 cut(s) | 220, 1033 |
| Hpy166II | GTNNAC | 5 cut(s) | 70, 192, 586, 775, 826 |
| Hpy188I | TCNGA | 4 cut(s) | 103, 210, 325, 431 |
| Hpy188III | TCNNGA | 6 cut(s) | 86, 119, 246, 518, 839, 848 |
| Hpy8I | GTNNAC | 5 cut(s) | 70, 192, 586, 775, 826 |
| HpyAV | CCTTC | 5 cut(s) | 298, 810, 1028, 1068, 1247 |
| HpyCH4III | ACNGT | 2 cut(s) | 22, 262 |
| HpyCH4IV | ACGT | 2 cut(s) | 91, 822 |
| HpyCH4V | TGCA | 4 cut(s) | 215, 559, 728, 745 |
| HpyF3I | CTNAG | 2 cut(s) | 428, 1044 |
| HpySE526I | ACGT | 2 cut(s) | 91, 822 |
| Hsp92II | CATG | 6 cut(s) | 122, 352, 488, 521, 1105, 1225 |
| Kpn2I | TCCGGA | 1 cut(s) | 838 |
| KpnI | GGTACC | 1 cut(s) | 321 |
| Kzo9I | GATC | 3 cut(s) | 106, 121, 801 |
| Lsp1109I | GCAGC | 5 cut(s) | 108, 586, 712, 729, 956 |
| LweI | GCATC | 1 cut(s) | 1115 |
| MaeI | CTAG | 6 cut(s) | 896, 911, 1053, 1119, 1167, 1209 |
| MaeII | ACGT | 2 cut(s) | 91, 822 |
| MaeIII | GTNAC | 2 cut(s) | 16, 1171 |
| MalI | GATC | 3 cut(s) | 108, 123, 803 |
| MboI | GATC | 3 cut(s) | 106, 121, 801 |
| MboII | GAAGA | 7 cut(s) | 350, 575, 825, 827, 886, 1117, 1207 |
| MfeI | CAATTG | 1 cut(s) | 435 |
| MlsI | TGGCCA | 1 cut(s) | 659 |
| MluCI | AATT | 7 cut(s) | 151, 159, 275, 333, 344, 435, 522 |
| MluNI | TGGCCA | 1 cut(s) | 659 |
| MlyI | GAGTC | 2 cut(s) | 676, 853 |
| MmeI | TCCRAC | 1 cut(s) | 1011 |
| Mox20I | TGGCCA | 1 cut(s) | 659 |
| MroI | TCCGGA | 1 cut(s) | 838 |
| MroXI | GAANNNNTTC | 2 cut(s) | 513, 609 |
| MscI | TGGCCA | 1 cut(s) | 659 |
| MseI | TTAA | 2 cut(s) | 959, 1007 |
| MslI | CAYNNNNRTG | 1 cut(s) | 765 |
| Msp20I | TGGCCA | 1 cut(s) | 659 |
| MspA1I | CMGCKG | 1 cut(s) | 947 |
| MspI | CCGG | 2 cut(s) | 839, 888 |
| MspR9I | CCNGG | 1 cut(s) | 717 |
| MunI | CAATTG | 1 cut(s) | 435 |
| Mva1269I | GAATGC | 2 cut(s) | 572, 792 |
| MvaI | CCWGG | 1 cut(s) | 717 |
| NcoI | CCATGG | 1 cut(s) | 484 |
| NdeII | GATC | 3 cut(s) | 106, 121, 801 |
| NlaIII | CATG | 6 cut(s) | 122, 352, 488, 521, 1105, 1225 |
| NlaIV | GGNNCC | 2 cut(s) | 289, 319 |
| NmuCI | GTSAC | 2 cut(s) | 16, 1171 |
| NspV | TTCGAA | 1 cut(s) | 507 |
| OliI | CACNNNNGTG | 1 cut(s) | 765 |
| PagI | TCATGA | 2 cut(s) | 118, 517 |
| PceI | AGGCCT | 1 cut(s) | 1003 |
| PctI | GAATGC | 2 cut(s) | 572, 792 |
| PdmI | GAANNNNTTC | 2 cut(s) | 513, 609 |
| PfeI | GAWTC | 6 cut(s) | 376, 504, 1048, 1136, 1178, 1226 |
| PkrI | GCNGC | 5 cut(s) | 98, 576, 727, 744, 946 |
| PleI | GAGTC | 2 cut(s) | 676, 852 |
| PpsI | GAGTC | 2 cut(s) | 676, 852 |
| PpuMI | RGGWCCY | 1 cut(s) | 307 |
| PsiI | TTATAA | 1 cut(s) | 267 |
| Psp5II | RGGWCCY | 1 cut(s) | 307 |
| Psp6I | CCWGG | 1 cut(s) | 715 |
| PspFI | CCCAGC | 1 cut(s) | 238 |
| PspGI | CCWGG | 1 cut(s) | 715 |
| PspN4I | GGNNCC | 2 cut(s) | 289, 319 |
| PspPI | GGNCC | 2 cut(s) | 307, 990 |
| PspPPI | RGGWCCY | 1 cut(s) | 307 |
| PstI | CTGCAG | 1 cut(s) | 747 |
| RsaI | GTAC | 3 cut(s) | 259, 319, 776 |
| RsaNI | GTAC | 3 cut(s) | 258, 318, 775 |
| RseI | CAYNNNNRTG | 1 cut(s) | 765 |
| SaqAI | TTAA | 2 cut(s) | 959, 1007 |
| SatI | GCNGC | 5 cut(s) | 97, 575, 726, 743, 945 |
| Sau3AI | GATC | 3 cut(s) | 106, 121, 801 |
| Sau96I | GGNCC | 2 cut(s) | 307, 990 |
| SchI | GAGTC | 2 cut(s) | 676, 853 |
| ScrFI | CCNGG | 1 cut(s) | 717 |
| SfaNI | GCATC | 1 cut(s) | 1115 |
| SfcI | CTRYAG | 1 cut(s) | 743 |
| SfuI | TTCGAA | 1 cut(s) | 507 |
| SinI | GGWCC | 1 cut(s) | 307 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 765 |
| SpeI | ACTAGT | 3 cut(s) | 1118, 1166, 1208 |
| Sse9I | AATT | 7 cut(s) | 151, 159, 275, 333, 344, 435, 522 |
| SseBI | AGGCCT | 1 cut(s) | 1003 |
| SsiI | CCGC | 3 cut(s) | 353, 527, 947 |
| SspI | AATATT | 1 cut(s) | 610 |
| SspMI | CTAG | 6 cut(s) | 896, 911, 1053, 1119, 1167, 1209 |
| StuI | AGGCCT | 1 cut(s) | 1003 |
| StyD4I | CCNGG | 1 cut(s) | 715 |
| StyI | CCWWGG | 3 cut(s) | 367, 484, 860 |
| TaaI | ACNGT | 2 cut(s) | 22, 262 |
| TaiI | ACGT | 2 cut(s) | 94, 825 |
| TaqI | TCGA | 6 cut(s) | 5, 134, 507, 847, 1128, 1218 |
| TasI | AATT | 7 cut(s) | 151, 159, 275, 333, 344, 435, 522 |
| TatI | WGTACW | 1 cut(s) | 774 |
| TfiI | GAWTC | 6 cut(s) | 376, 504, 1048, 1136, 1178, 1226 |
| Tru1I | TTAA | 2 cut(s) | 959, 1007 |
| Tru9I | TTAA | 2 cut(s) | 959, 1007 |
| TscAI | CASTG | 2 cut(s) | 306, 752 |
| TseFI | GTSAC | 2 cut(s) | 16, 1171 |
| TseI | GCWGC | 5 cut(s) | 96, 574, 725, 742, 944 |
| Tsp45I | GTSAC | 2 cut(s) | 16, 1171 |
| TspDTI | ATGAA | 4 cut(s) | 337, 368, 506, 534 |
| TspGWI | ACGGA | 2 cut(s) | 1148, 1190 |
| TspRI | CASTG | 2 cut(s) | 306, 752 |
| VpaK11BI | GGWCC | 1 cut(s) | 307 |
| XapI | RAATTY | 1 cut(s) | 275 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 867 |
| XmiI | GTMKAC | 2 cut(s) | 69, 825 |
| XmnI | GAANNNNTTC | 2 cut(s) | 513, 609 |
| XspI | CTAG | 6 cut(s) | 896, 911, 1053, 1119, 1167, 1209 |