Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 123 |
| Acc65I | GGTACC | 1 cut(s) | 173 |
| AccB1I | GGYRCC | 1 cut(s) | 173 |
| AccIII | TCCGGA | 1 cut(s) | 694 |
| AciI | CCGC | 3 cut(s) | 209, 383, 601 |
| AclWI | GGATC | 1 cut(s) | 106 |
| AcoI | YGGCCR | 1 cut(s) | 513 |
| AcsI | RAATTY | 2 cut(s) | 8, 903 |
| AfaI | GTAC | 4 cut(s) | 175, 220, 632, 1033 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 228 |
| AgsI | TTSAA | 7 cut(s) | 194, 244, 365, 740, 902, 918, 1061 |
| AhlI | ACTAGT | 1 cut(s) | 974 |
| AjnI | CCWGG | 2 cut(s) | 571, 943 |
| AleI | CACNNNNGTG | 2 cut(s) | 621, 977 |
| AluBI | AGCT | 9 cut(s) | 370, 395, 500, 520, 636, 743, 760, 824, 1020 |
| AluI | AGCT | 9 cut(s) | 370, 395, 500, 520, 636, 743, 760, 824, 1020 |
| Alw21I | GWGCWC | 2 cut(s) | 292, 898 |
| Alw26I | GTCTC | 1 cut(s) | 271 |
| AlwI | GGATC | 1 cut(s) | 106 |
| Aor13HI | TCCGGA | 1 cut(s) | 694 |
| AoxI | GGCC | 3 cut(s) | 513, 845, 857 |
| ApeKI | GCWGC | 3 cut(s) | 430, 581, 598 |
| ApoI | RAATTY | 2 cut(s) | 8, 903 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 369, 465 |
| Asp718I | GGTACC | 1 cut(s) | 173 |
| AsuHPI | GGTGA | 2 cut(s) | 76, 889 |
| BalI | TGGCCA | 1 cut(s) | 515 |
| BanI | GGYRCC | 1 cut(s) | 173 |
| BbsI | GAAGAC | 1 cut(s) | 248 |
| Bbv12I | GWGCWC | 2 cut(s) | 292, 898 |
| BbvI | GCAGC | 3 cut(s) | 442, 568, 585 |
| BccI | CCATC | 1 cut(s) | 47 |
| BciT130I | CCWGG | 2 cut(s) | 573, 945 |
| BcoDI | GTCTC | 1 cut(s) | 271 |
| BcuI | ACTAGT | 1 cut(s) | 974 |
| BfaI | CTAG | 4 cut(s) | 396, 680, 975, 1075 |
| BisI | GCNGC | 3 cut(s) | 431, 582, 599 |
| BlsI | GCNGC | 3 cut(s) | 432, 583, 600 |
| Bme1390I | CCNGG | 2 cut(s) | 573, 945 |
| BmiI | GGNNCC | 3 cut(s) | 145, 175, 677 |
| BmrFI | CCNGG | 2 cut(s) | 573, 945 |
| BmrI | ACTGGG | 1 cut(s) | 761 |
| BmsI | GCATC | 1 cut(s) | 930 |
| BmuI | ACTGGG | 1 cut(s) | 761 |
| BpiI | GAAGAC | 1 cut(s) | 248 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 553, 585 |
| BsaJI | CCNNGG | 3 cut(s) | 223, 340, 716 |
| BsaWI | WCCGGW | 1 cut(s) | 694 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 228 |
| Bse1I | ACTGG | 3 cut(s) | 713, 756, 1015 |
| Bse3DI | GCAATG | 1 cut(s) | 378 |
| BseAI | TCCGGA | 1 cut(s) | 694 |
| BseBI | CCWGG | 2 cut(s) | 573, 945 |
| BseDI | CCNNGG | 3 cut(s) | 223, 340, 716 |
| BseGI | GGATG | 2 cut(s) | 481, 1067 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 228 |
| BseMI | GCAATG | 1 cut(s) | 378 |
| BseMII | CTCAG | 2 cut(s) | 298, 840 |
| BseNI | ACTGG | 3 cut(s) | 713, 756, 1015 |
| BseRI | GAGGAG | 1 cut(s) | 797 |
| BseXI | GCAGC | 3 cut(s) | 442, 568, 585 |
| BseYI | CCCAGC | 1 cut(s) | 94 |
| BsgI | GTGCAG | 2 cut(s) | 135, 567 |
| BshFI | GGCC | 3 cut(s) | 515, 847, 859 |
| BshNI | GGYRCC | 1 cut(s) | 173 |
| BsiHKAI | GWGCWC | 2 cut(s) | 292, 898 |
| BsiSI | CCGG | 1 cut(s) | 695 |
| BslFI | GGGAC | 1 cut(s) | 403 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 228 |
| BsmAI | GTCTC | 1 cut(s) | 271 |
| BsmFI | GGGAC | 1 cut(s) | 403 |
| BsmI | GAATGC | 1 cut(s) | 428 |
| BsnI | GGCC | 3 cut(s) | 515, 847, 859 |
| Bsp1286I | GDGCHC | 2 cut(s) | 292, 898 |
| Bsp13I | TCCGGA | 1 cut(s) | 694 |
| Bsp1407I | TGTACA | 1 cut(s) | 630 |
| Bsp143I | GATC | 2 cut(s) | 98, 657 |
| Bsp19I | CCATGG | 1 cut(s) | 340 |
| BspACI | CCGC | 3 cut(s) | 209, 383, 601 |
| BspANI | GGCC | 3 cut(s) | 515, 847, 859 |
| BspCNI | CTCAG | 2 cut(s) | 297, 841 |
| BspEI | TCCGGA | 1 cut(s) | 694 |
| BspHI | TCATGA | 2 cut(s) | 373, 1041 |
| BspLI | GGNNCC | 3 cut(s) | 145, 175, 677 |
| BspPI | GGATC | 1 cut(s) | 106 |
| BspT107I | GGYRCC | 1 cut(s) | 173 |
| BsrDI | GCAATG | 1 cut(s) | 378 |
| BsrGI | TGTACA | 1 cut(s) | 630 |
| BsrI | ACTGG | 3 cut(s) | 713, 756, 1015 |
| BssECI | CCNNGG | 3 cut(s) | 223, 340, 716 |
| BssMI | GATC | 2 cut(s) | 98, 657 |
| BssT1I | CCWWGG | 2 cut(s) | 223, 340 |
| Bst2UI | CCWGG | 2 cut(s) | 573, 945 |
| Bst4CI | ACNGT | 2 cut(s) | 317, 701 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 940 |
| BstAUI | TGTACA | 1 cut(s) | 630 |
| BstC8I | GCNNGC | 1 cut(s) | 428 |
| BstDEI | CTNAG | 2 cut(s) | 284, 849 |
| BstDSI | CCRYGG | 1 cut(s) | 340 |
| BstF5I | GGATG | 2 cut(s) | 481, 1067 |
| BstKTI | GATC | 2 cut(s) | 101, 660 |
| BstMAI | GTCTC | 1 cut(s) | 271 |
| BstMBI | GATC | 2 cut(s) | 98, 657 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 940 |
| BstNI | CCWGG | 2 cut(s) | 573, 945 |
| BstSCI | CCNGG | 2 cut(s) | 571, 943 |
| BstV1I | GCAGC | 3 cut(s) | 442, 568, 585 |
| BstV2I | GAAGAC | 1 cut(s) | 248 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 94, 594 |
| BsuRI | GGCC | 3 cut(s) | 515, 847, 859 |
| BtgI | CCRYGG | 1 cut(s) | 340 |
| BtsCI | GGATG | 2 cut(s) | 481, 1067 |
| BtsIMutI | CAGTG | 1 cut(s) | 987 |
| Cac8I | GCNNGC | 1 cut(s) | 428 |
| CciI | TCATGA | 2 cut(s) | 373, 1041 |
| Csp6I | GTAC | 4 cut(s) | 174, 219, 631, 1032 |
| CviAII | CATG | 7 cut(s) | 22, 205, 260, 341, 374, 958, 1042 |
| CviQI | GTAC | 4 cut(s) | 174, 219, 631, 1032 |
| DdeI | CTNAG | 2 cut(s) | 284, 849 |
| DpnI | GATC | 2 cut(s) | 100, 659 |
| DpnII | GATC | 2 cut(s) | 98, 657 |
| EaeI | YGGCCR | 1 cut(s) | 513 |
| EciI | GGCGGA | 1 cut(s) | 224 |
| Eco130I | CCWWGG | 2 cut(s) | 223, 340 |
| Eco147I | AGGCCT | 2 cut(s) | 847, 859 |
| EcoRII | CCWGG | 2 cut(s) | 571, 943 |
| EcoT14I | CCWWGG | 2 cut(s) | 223, 340 |
| ErhI | CCWWGG | 2 cut(s) | 223, 340 |
| FaeI | CATG | 7 cut(s) | 25, 208, 263, 344, 377, 961, 1045 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 882, 914 |
| FaqI | GGGAC | 1 cut(s) | 403 |
| FatI | CATG | 7 cut(s) | 21, 204, 259, 340, 373, 957, 1041 |
| Fnu4HI | GCNGC | 3 cut(s) | 431, 582, 599 |
| FokI | GGATG | 2 cut(s) | 488, 1054 |
| Fsp4HI | GCNGC | 3 cut(s) | 431, 582, 599 |
| FspBI | CTAG | 4 cut(s) | 396, 680, 975, 1075 |
| GluI | GCNGC | 3 cut(s) | 431, 582, 599 |
| GsaI | CCCAGC | 1 cut(s) | 98 |
| HaeIII | GGCC | 3 cut(s) | 515, 847, 859 |
| HapII | CCGG | 1 cut(s) | 695 |
| Hin1II | CATG | 7 cut(s) | 25, 208, 263, 344, 377, 961, 1045 |
| HindIII | AAGCTT | 1 cut(s) | 368 |
| HinfI | GANTC | 3 cut(s) | 232, 688, 992 |
| HpaII | CCGG | 1 cut(s) | 695 |
| HphI | GGTGA | 2 cut(s) | 76, 889 |
| Hpy166II | GTNNAC | 4 cut(s) | 320, 442, 481, 631 |
| Hpy188I | TCNGA | 7 cut(s) | 15, 44, 66, 168, 181, 287, 717 |
| Hpy188III | TCNNGA | 5 cut(s) | 102, 374, 695, 704, 1042 |
| Hpy8I | GTNNAC | 4 cut(s) | 320, 442, 481, 631 |
| HpyAV | CCTTC | 3 cut(s) | 666, 884, 924 |
| HpyCH4III | ACNGT | 2 cut(s) | 317, 701 |
| HpyCH4V | TGCA | 4 cut(s) | 71, 116, 415, 584 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 940 |
| HpyF3I | CTNAG | 2 cut(s) | 284, 849 |
| Hsp92II | CATG | 7 cut(s) | 25, 208, 263, 344, 377, 961, 1045 |
| Kpn2I | TCCGGA | 1 cut(s) | 694 |
| KpnI | GGTACC | 1 cut(s) | 177 |
| Kzo9I | GATC | 2 cut(s) | 98, 657 |
| LmnI | GCTCC | 1 cut(s) | 893 |
| Lsp1109I | GCAGC | 3 cut(s) | 442, 568, 585 |
| LweI | GCATC | 1 cut(s) | 930 |
| MaeI | CTAG | 4 cut(s) | 396, 680, 975, 1075 |
| MalI | GATC | 2 cut(s) | 100, 659 |
| MboI | GATC | 2 cut(s) | 98, 657 |
| MboII | GAAGA | 7 cut(s) | 206, 248, 431, 681, 683, 742, 1057 |
| MhlI | GDGCHC | 2 cut(s) | 292, 898 |
| MlsI | TGGCCA | 1 cut(s) | 515 |
| MluCI | AATT | 5 cut(s) | 8, 189, 200, 903, 918 |
| MluNI | TGGCCA | 1 cut(s) | 515 |
| MmeI | TCCRAC | 1 cut(s) | 549 |
| MnlI | CCTC | 8 cut(s) | 98, 187, 293, 555, 711, 775, 849, 1006 |
| Mox20I | TGGCCA | 1 cut(s) | 515 |
| MroI | TCCGGA | 1 cut(s) | 694 |
| MroXI | GAANNNNTTC | 2 cut(s) | 369, 465 |
| MscI | TGGCCA | 1 cut(s) | 515 |
| MseI | TTAA | 1 cut(s) | 921 |
| MslI | CAYNNNNRTG | 2 cut(s) | 621, 977 |
| Msp20I | TGGCCA | 1 cut(s) | 515 |
| MspI | CCGG | 1 cut(s) | 695 |
| MspR9I | CCNGG | 2 cut(s) | 573, 945 |
| Mva1269I | GAATGC | 1 cut(s) | 428 |
| MvaI | CCWGG | 2 cut(s) | 573, 945 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 940 |
| NcoI | CCATGG | 1 cut(s) | 340 |
| NdeII | GATC | 2 cut(s) | 98, 657 |
| NlaIII | CATG | 7 cut(s) | 25, 208, 263, 344, 377, 961, 1045 |
| NlaIV | GGNNCC | 3 cut(s) | 145, 175, 677 |
| OliI | CACNNNNGTG | 2 cut(s) | 621, 977 |
| PagI | TCATGA | 2 cut(s) | 373, 1041 |
| PceI | AGGCCT | 2 cut(s) | 847, 859 |
| PctI | GAATGC | 1 cut(s) | 428 |
| PdmI | GAANNNNTTC | 2 cut(s) | 369, 465 |
| PfeI | GAWTC | 3 cut(s) | 232, 688, 992 |
| PkrI | GCNGC | 3 cut(s) | 432, 583, 600 |
| PsiI | TTATAA | 1 cut(s) | 123 |
| Psp6I | CCWGG | 2 cut(s) | 571, 943 |
| PspFI | CCCAGC | 1 cut(s) | 94 |
| PspGI | CCWGG | 2 cut(s) | 571, 943 |
| PspN4I | GGNNCC | 3 cut(s) | 145, 175, 677 |
| RsaI | GTAC | 4 cut(s) | 175, 220, 632, 1033 |
| RsaNI | GTAC | 4 cut(s) | 174, 219, 631, 1032 |
| RseI | CAYNNNNRTG | 2 cut(s) | 621, 977 |
| SaqAI | TTAA | 1 cut(s) | 921 |
| SatI | GCNGC | 3 cut(s) | 431, 582, 599 |
| Sau3AI | GATC | 2 cut(s) | 98, 657 |
| ScrFI | CCNGG | 2 cut(s) | 573, 945 |
| SduI | GDGCHC | 2 cut(s) | 292, 898 |
| SfaNI | GCATC | 1 cut(s) | 930 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 621, 977 |
| SpeI | ACTAGT | 1 cut(s) | 974 |
| Sse9I | AATT | 5 cut(s) | 8, 189, 200, 903, 918 |
| SseBI | AGGCCT | 2 cut(s) | 847, 859 |
| SsiI | CCGC | 3 cut(s) | 209, 383, 601 |
| SspI | AATATT | 2 cut(s) | 132, 466 |
| SspMI | CTAG | 4 cut(s) | 396, 680, 975, 1075 |
| StuI | AGGCCT | 2 cut(s) | 847, 859 |
| StyD4I | CCNGG | 2 cut(s) | 571, 943 |
| StyI | CCWWGG | 2 cut(s) | 223, 340 |
| TaaI | ACNGT | 2 cut(s) | 317, 701 |
| TaqI | TCGA | 1 cut(s) | 984 |
| TasI | AATT | 5 cut(s) | 8, 189, 200, 903, 918 |
| TatI | WGTACW | 1 cut(s) | 630 |
| TfiI | GAWTC | 3 cut(s) | 232, 688, 992 |
| Tru1I | TTAA | 1 cut(s) | 921 |
| Tru9I | TTAA | 1 cut(s) | 921 |
| TscAI | CASTG | 1 cut(s) | 994 |
| TseI | GCWGC | 3 cut(s) | 430, 581, 598 |
| TspDTI | ATGAA | 5 cut(s) | 193, 224, 248, 362, 1058 |
| TspRI | CASTG | 1 cut(s) | 994 |
| XapI | RAATTY | 2 cut(s) | 8, 903 |
| XmnI | GAANNNNTTC | 2 cut(s) | 369, 465 |
| XspI | CTAG | 4 cut(s) | 396, 680, 975, 1075 |