Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 357 |
| Acc65I | GGTACC | 1 cut(s) | 407 |
| AccB1I | GGYRCC | 1 cut(s) | 407 |
| AciI | CCGC | 4 cut(s) | 26, 69, 443, 1190 |
| AclWI | GGATC | 1 cut(s) | 215 |
| AcoI | YGGCCR | 1 cut(s) | 738 |
| AcsI | RAATTY | 2 cut(s) | 706, 1121 |
| AfaI | GTAC | 3 cut(s) | 409, 454, 857 |
| AfeI | AGCGCT | 1 cut(s) | 516 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 947 |
| AgsI | TTSAA | 3 cut(s) | 176, 965, 1143 |
| AjiI | CACGTC | 1 cut(s) | 194 |
| AjnI | CCWGG | 2 cut(s) | 682, 796 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 594, 626, 921, 953 |
| AleI | CACNNNNGTG | 1 cut(s) | 846 |
| AluBI | AGCT | 8 cut(s) | 213, 295, 595, 620, 745, 861, 968, 1049 |
| AluI | AGCT | 8 cut(s) | 213, 295, 595, 620, 745, 861, 968, 1049 |
| Alw26I | GTCTC | 2 cut(s) | 494, 1226 |
| AlwI | GGATC | 1 cut(s) | 215 |
| Aor51HI | AGCGCT | 1 cut(s) | 516 |
| AoxI | GGCC | 2 cut(s) | 738, 1082 |
| ApeKI | GCWGC | 5 cut(s) | 210, 655, 806, 1025, 1187 |
| ApoI | RAATTY | 2 cut(s) | 706, 1121 |
| AseI | ATTAAT | 1 cut(s) | 1146 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 594, 710 |
| Asp718I | GGTACC | 1 cut(s) | 407 |
| AspLEI | GCGC | 1 cut(s) | 517 |
| AspS9I | GGNCC | 1 cut(s) | 1083 |
| AsuHPI | GGTGA | 2 cut(s) | 983, 1114 |
| AsuII | TTCGAA | 1 cut(s) | 588 |
| BalI | TGGCCA | 1 cut(s) | 740 |
| BanI | GGYRCC | 1 cut(s) | 407 |
| BbvI | GCAGC | 5 cut(s) | 222, 667, 793, 1037, 1199 |
| BccI | CCATC | 2 cut(s) | 281, 945 |
| BceAI | ACGGC | 4 cut(s) | 5, 17, 41, 47 |
| BciT130I | CCWGG | 2 cut(s) | 684, 798 |
| BclI | TGATCA | 1 cut(s) | 196 |
| BcoDI | GTCTC | 2 cut(s) | 494, 1226 |
| BfaI | CTAG | 4 cut(s) | 401, 621, 977, 1134 |
| BfmI | CTRYAG | 1 cut(s) | 290 |
| BfoI | RGCGCY | 1 cut(s) | 518 |
| BglII | AGATCT | 1 cut(s) | 576 |
| BisI | GCNGC | 6 cut(s) | 69, 211, 656, 807, 1026, 1188 |
| BlsI | GCNGC | 6 cut(s) | 70, 212, 657, 808, 1027, 1189 |
| Bme1390I | CCNGG | 2 cut(s) | 684, 798 |
| BmgBI | CACGTC | 1 cut(s) | 194 |
| BmgT120I | GGNCC | 1 cut(s) | 1083 |
| BmiI | GGNNCC | 2 cut(s) | 409, 902 |
| BmrFI | CCNGG | 2 cut(s) | 684, 798 |
| BmsI | GCATC | 1 cut(s) | 1155 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 778, 810 |
| Bpu14I | TTCGAA | 1 cut(s) | 588 |
| BsaBI | GATNNNNATC | 1 cut(s) | 164 |
| BsaJI | CCNNGG | 4 cut(s) | 411, 493, 565, 941 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 102, 132 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 947 |
| Bse1I | ACTGG | 2 cut(s) | 938, 1216 |
| Bse8I | GATNNNNATC | 1 cut(s) | 164 |
| BseBI | CCWGG | 2 cut(s) | 684, 798 |
| BseDI | CCNNGG | 4 cut(s) | 411, 493, 565, 941 |
| BseGI | GGATG | 2 cut(s) | 706, 956 |
| BseJI | GATNNNNATC | 1 cut(s) | 164 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 947 |
| BseNI | ACTGG | 2 cut(s) | 938, 1216 |
| BseXI | GCAGC | 5 cut(s) | 222, 667, 793, 1037, 1199 |
| BseYI | CCCAGC | 1 cut(s) | 823 |
| BsgI | GTGCAG | 2 cut(s) | 135, 792 |
| BshFI | GGCC | 2 cut(s) | 740, 1084 |
| BshNI | GGYRCC | 1 cut(s) | 407 |
| BslFI | GGGAC | 2 cut(s) | 628, 770 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 947 |
| BsmAI | GTCTC | 2 cut(s) | 494, 1226 |
| BsmBI | CGTCTC | 1 cut(s) | 494 |
| BsmFI | GGGAC | 2 cut(s) | 628, 770 |
| BsmI | GAATGC | 2 cut(s) | 653, 873 |
| BsnI | GGCC | 2 cut(s) | 740, 1084 |
| Bsp119I | TTCGAA | 1 cut(s) | 588 |
| Bsp1407I | TGTACA | 1 cut(s) | 855 |
| Bsp143I | GATC | 3 cut(s) | 196, 220, 576 |
| Bsp19I | CCATGG | 2 cut(s) | 493, 565 |
| BspACI | CCGC | 4 cut(s) | 26, 69, 443, 1190 |
| BspANI | GGCC | 2 cut(s) | 740, 1084 |
| BspHI | TCATGA | 1 cut(s) | 598 |
| BspLI | GGNNCC | 2 cut(s) | 409, 902 |
| BspPI | GGATC | 1 cut(s) | 215 |
| BspT104I | TTCGAA | 1 cut(s) | 588 |
| BspT107I | GGYRCC | 1 cut(s) | 407 |
| BsrGI | TGTACA | 1 cut(s) | 855 |
| BsrI | ACTGG | 2 cut(s) | 938, 1216 |
| BssECI | CCNNGG | 4 cut(s) | 411, 493, 565, 941 |
| BssMI | GATC | 3 cut(s) | 196, 220, 576 |
| BssT1I | CCWWGG | 3 cut(s) | 493, 565, 941 |
| Bst2UI | CCWGG | 2 cut(s) | 684, 798 |
| Bst4CI | ACNGT | 1 cut(s) | 172 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 122, 1165 |
| BstAUI | TGTACA | 1 cut(s) | 855 |
| BstBI | TTCGAA | 1 cut(s) | 588 |
| BstC8I | GCNNGC | 1 cut(s) | 653 |
| BstDEI | CTNAG | 1 cut(s) | 580 |
| BstDSI | CCRYGG | 2 cut(s) | 493, 565 |
| BstF5I | GGATG | 2 cut(s) | 706, 956 |
| BstH2I | RGCGCY | 1 cut(s) | 518 |
| BstHHI | GCGC | 1 cut(s) | 517 |
| BstKTI | GATC | 3 cut(s) | 199, 223, 579 |
| BstMAI | GTCTC | 2 cut(s) | 494, 1226 |
| BstMBI | GATC | 3 cut(s) | 196, 220, 576 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 68, 122, 1165 |
| BstNI | CCWGG | 2 cut(s) | 684, 798 |
| BstSCI | CCNGG | 2 cut(s) | 682, 796 |
| BstSFI | CTRYAG | 1 cut(s) | 290 |
| BstV1I | GCAGC | 5 cut(s) | 222, 667, 793, 1037, 1199 |
| BstX2I | RGATCY | 1 cut(s) | 576 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 819 |
| BstYI | RGATCY | 1 cut(s) | 576 |
| BsuRI | GGCC | 2 cut(s) | 740, 1084 |
| BtgI | CCRYGG | 2 cut(s) | 493, 565 |
| BtrI | CACGTC | 1 cut(s) | 194 |
| BtsCI | GGATG | 2 cut(s) | 706, 956 |
| BtsI | GCAGTG | 2 cut(s) | 396, 999 |
| BtsIMutI | CAGTG | 2 cut(s) | 396, 999 |
| Cac8I | GCNNGC | 1 cut(s) | 653 |
| CciI | TCATGA | 1 cut(s) | 598 |
| CfoI | GCGC | 1 cut(s) | 517 |
| Cfr13I | GGNCC | 1 cut(s) | 1083 |
| Csp6I | GTAC | 3 cut(s) | 408, 453, 856 |
| CviAII | CATG | 7 cut(s) | 256, 439, 473, 494, 566, 599, 1183 |
| CviQI | GTAC | 3 cut(s) | 408, 453, 856 |
| DdeI | CTNAG | 1 cut(s) | 580 |
| DpnI | GATC | 3 cut(s) | 198, 222, 578 |
| DpnII | GATC | 3 cut(s) | 196, 220, 576 |
| EaeI | YGGCCR | 1 cut(s) | 738 |
| EciI | GGCGGA | 1 cut(s) | 458 |
| Eco130I | CCWWGG | 3 cut(s) | 493, 565, 941 |
| Eco47III | AGCGCT | 1 cut(s) | 516 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1083 |
| EcoRII | CCWGG | 2 cut(s) | 682, 796 |
| EcoT14I | CCWWGG | 3 cut(s) | 493, 565, 941 |
| ErhI | CCWWGG | 3 cut(s) | 493, 565, 941 |
| Esp3I | CGTCTC | 1 cut(s) | 494 |
| FaeI | CATG | 7 cut(s) | 259, 442, 476, 497, 569, 602, 1186 |
| FaqI | GGGAC | 2 cut(s) | 628, 770 |
| FatI | CATG | 7 cut(s) | 255, 438, 472, 493, 565, 598, 1182 |
| FbaI | TGATCA | 1 cut(s) | 196 |
| Fnu4HI | GCNGC | 6 cut(s) | 69, 211, 656, 807, 1026, 1188 |
| FokI | GGATG | 2 cut(s) | 713, 963 |
| Fsp4HI | GCNGC | 6 cut(s) | 69, 211, 656, 807, 1026, 1188 |
| FspBI | CTAG | 4 cut(s) | 401, 621, 977, 1134 |
| GlaI | GCGC | 1 cut(s) | 516 |
| GluI | GCNGC | 6 cut(s) | 69, 211, 656, 807, 1026, 1188 |
| GsaI | CCCAGC | 1 cut(s) | 827 |
| HaeII | RGCGCY | 1 cut(s) | 518 |
| HaeIII | GGCC | 2 cut(s) | 740, 1084 |
| HhaI | GCGC | 1 cut(s) | 517 |
| Hin1II | CATG | 7 cut(s) | 259, 442, 476, 497, 569, 602, 1186 |
| Hin6I | GCGC | 1 cut(s) | 515 |
| HinP1I | GCGC | 1 cut(s) | 515 |
| HindIII | AAGCTT | 1 cut(s) | 593 |
| HinfI | GANTC | 6 cut(s) | 95, 187, 466, 585, 1129, 1193 |
| HphI | GGTGA | 2 cut(s) | 983, 1114 |
| Hpy166II | GTNNAC | 5 cut(s) | 282, 455, 667, 856, 974 |
| Hpy188I | TCNGA | 7 cut(s) | 156, 201, 217, 225, 300, 415, 1075 |
| Hpy188III | TCNNGA | 4 cut(s) | 6, 336, 599, 880 |
| Hpy8I | GTNNAC | 5 cut(s) | 282, 455, 667, 856, 974 |
| Hpy99I | CGWCG | 1 cut(s) | 195 |
| HpyAV | CCTTC | 4 cut(s) | 388, 891, 1109, 1149 |
| HpyCH4III | ACNGT | 1 cut(s) | 172 |
| HpyCH4IV | ACGT | 1 cut(s) | 193 |
| HpyCH4V | TGCA | 6 cut(s) | 116, 305, 608, 640, 750, 809 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 68, 122, 1165 |
| HpyF3I | CTNAG | 1 cut(s) | 580 |
| HpySE526I | ACGT | 1 cut(s) | 193 |
| Hsp92II | CATG | 7 cut(s) | 259, 442, 476, 497, 569, 602, 1186 |
| HspAI | GCGC | 1 cut(s) | 515 |
| KpnI | GGTACC | 1 cut(s) | 411 |
| Ksp22I | TGATCA | 1 cut(s) | 196 |
| Kzo9I | GATC | 3 cut(s) | 196, 220, 576 |
| Lsp1109I | GCAGC | 5 cut(s) | 222, 667, 793, 1037, 1199 |
| LweI | GCATC | 1 cut(s) | 1155 |
| MaeI | CTAG | 4 cut(s) | 401, 621, 977, 1134 |
| MaeII | ACGT | 1 cut(s) | 193 |
| MaeIII | GTNAC | 2 cut(s) | 83, 101 |
| MalI | GATC | 3 cut(s) | 198, 222, 578 |
| MboI | GATC | 3 cut(s) | 196, 220, 576 |
| MboII | GAAGA | 4 cut(s) | 656, 703, 906, 967 |
| MflI | RGATCY | 1 cut(s) | 576 |
| MlsI | TGGCCA | 1 cut(s) | 740 |
| MluCI | AATT | 9 cut(s) | 10, 241, 423, 434, 478, 603, 706, 1121, 1143 |
| MluNI | TGGCCA | 1 cut(s) | 740 |
| MmeI | TCCRAC | 3 cut(s) | 248, 1053, 1092 |
| Mox20I | TGGCCA | 1 cut(s) | 740 |
| MroXI | GAANNNNTTC | 2 cut(s) | 594, 710 |
| MscI | TGGCCA | 1 cut(s) | 740 |
| MseI | TTAA | 5 cut(s) | 912, 1040, 1088, 1146, 1237 |
| MslI | CAYNNNNRTG | 1 cut(s) | 846 |
| Msp20I | TGGCCA | 1 cut(s) | 740 |
| MspA1I | CMGCKG | 1 cut(s) | 1190 |
| MspR9I | CCNGG | 2 cut(s) | 684, 798 |
| Mva1269I | GAATGC | 2 cut(s) | 653, 873 |
| MvaI | CCWGG | 2 cut(s) | 684, 798 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 68, 122, 1165 |
| NcoI | CCATGG | 2 cut(s) | 493, 565 |
| NdeII | GATC | 3 cut(s) | 196, 220, 576 |
| NlaIII | CATG | 7 cut(s) | 259, 442, 476, 497, 569, 602, 1186 |
| NlaIV | GGNNCC | 2 cut(s) | 409, 902 |
| NspV | TTCGAA | 1 cut(s) | 588 |
| OliI | CACNNNNGTG | 1 cut(s) | 846 |
| PagI | TCATGA | 1 cut(s) | 598 |
| PctI | GAATGC | 2 cut(s) | 653, 873 |
| PdmI | GAANNNNTTC | 2 cut(s) | 594, 710 |
| PfeI | GAWTC | 6 cut(s) | 95, 187, 466, 585, 1129, 1193 |
| PkrI | GCNGC | 6 cut(s) | 70, 212, 657, 808, 1027, 1189 |
| PshBI | ATTAAT | 1 cut(s) | 1146 |
| PsiI | TTATAA | 1 cut(s) | 357 |
| Psp6I | CCWGG | 2 cut(s) | 682, 796 |
| PspFI | CCCAGC | 1 cut(s) | 823 |
| PspGI | CCWGG | 2 cut(s) | 682, 796 |
| PspN4I | GGNNCC | 2 cut(s) | 409, 902 |
| PspPI | GGNCC | 1 cut(s) | 1083 |
| PsuI | RGATCY | 1 cut(s) | 576 |
| RsaI | GTAC | 3 cut(s) | 409, 454, 857 |
| RsaNI | GTAC | 3 cut(s) | 408, 453, 856 |
| RseI | CAYNNNNRTG | 1 cut(s) | 846 |
| SaqAI | TTAA | 5 cut(s) | 912, 1040, 1088, 1146, 1237 |
| SatI | GCNGC | 6 cut(s) | 69, 211, 656, 807, 1026, 1188 |
| Sau3AI | GATC | 3 cut(s) | 196, 220, 576 |
| Sau96I | GGNCC | 1 cut(s) | 1083 |
| ScrFI | CCNGG | 2 cut(s) | 684, 798 |
| SfaNI | GCATC | 1 cut(s) | 1155 |
| SfcI | CTRYAG | 1 cut(s) | 290 |
| SfuI | TTCGAA | 1 cut(s) | 588 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 846 |
| Sse9I | AATT | 9 cut(s) | 10, 241, 423, 434, 478, 603, 706, 1121, 1143 |
| SsiI | CCGC | 4 cut(s) | 26, 69, 443, 1190 |
| SspI | AATATT | 1 cut(s) | 691 |
| SspMI | CTAG | 4 cut(s) | 401, 621, 977, 1134 |
| StyD4I | CCNGG | 2 cut(s) | 682, 796 |
| StyI | CCWWGG | 3 cut(s) | 493, 565, 941 |
| TaaI | ACNGT | 1 cut(s) | 172 |
| TaiI | ACGT | 1 cut(s) | 196 |
| TaqI | TCGA | 5 cut(s) | 5, 98, 190, 588, 879 |
| TasI | AATT | 9 cut(s) | 10, 241, 423, 434, 478, 603, 706, 1121, 1143 |
| TatI | WGTACW | 2 cut(s) | 452, 855 |
| TauI | GCSGC | 1 cut(s) | 71 |
| TfiI | GAWTC | 6 cut(s) | 95, 187, 466, 585, 1129, 1193 |
| Tru1I | TTAA | 5 cut(s) | 912, 1040, 1088, 1146, 1237 |
| Tru9I | TTAA | 5 cut(s) | 912, 1040, 1088, 1146, 1237 |
| TscAI | CASTG | 2 cut(s) | 396, 999 |
| TseI | GCWGC | 5 cut(s) | 210, 655, 806, 1025, 1187 |
| TspDTI | ATGAA | 4 cut(s) | 427, 458, 587, 615 |
| TspRI | CASTG | 2 cut(s) | 396, 999 |
| VspI | ATTAAT | 1 cut(s) | 1146 |
| XapI | RAATTY | 2 cut(s) | 706, 1121 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 948 |
| XmnI | GAANNNNTTC | 2 cut(s) | 594, 710 |
| XspI | CTAG | 4 cut(s) | 401, 621, 977, 1134 |