Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 123 |
| Acc65I | GGTACC | 1 cut(s) | 173 |
| AccB1I | GGYRCC | 1 cut(s) | 173 |
| AccI | GTMKAC | 1 cut(s) | 1073 |
| AciI | CCGC | 1 cut(s) | 212 |
| AclWI | GGATC | 1 cut(s) | 189 |
| AcoI | YGGCCR | 1 cut(s) | 516 |
| AcsI | RAATTY | 3 cut(s) | 60, 484, 899 |
| AfaI | GTAC | 3 cut(s) | 175, 223, 635 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 725, 858 |
| AgsI | TTSAA | 3 cut(s) | 743, 921, 1064 |
| AhlI | ACTAGT | 1 cut(s) | 977 |
| AjnI | CCWGG | 2 cut(s) | 460, 574 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 372, 404, 699, 731 |
| AleI | CACNNNNGTG | 1 cut(s) | 624 |
| AluBI | AGCT | 7 cut(s) | 286, 373, 398, 523, 639, 746, 827 |
| AluI | AGCT | 7 cut(s) | 286, 373, 398, 523, 639, 746, 827 |
| Alw21I | GWGCWC | 1 cut(s) | 295 |
| Alw26I | GTCTC | 2 cut(s) | 263, 1028 |
| AlwI | GGATC | 1 cut(s) | 189 |
| AoxI | GGCC | 2 cut(s) | 516, 860 |
| ApeKI | GCWGC | 4 cut(s) | 433, 584, 803, 965 |
| ApoI | RAATTY | 3 cut(s) | 60, 484, 899 |
| AseI | ATTAAT | 1 cut(s) | 924 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 372, 488 |
| Asp718I | GGTACC | 1 cut(s) | 173 |
| AspS9I | GGNCC | 2 cut(s) | 849, 861 |
| AsuHPI | GGTGA | 3 cut(s) | 76, 761, 892 |
| AsuII | TTCGAA | 1 cut(s) | 366 |
| AvaII | GGWCC | 1 cut(s) | 849 |
| BalI | TGGCCA | 1 cut(s) | 518 |
| BanI | GGYRCC | 1 cut(s) | 173 |
| Bbv12I | GWGCWC | 1 cut(s) | 295 |
| BbvI | GCAGC | 4 cut(s) | 445, 571, 815, 977 |
| BccI | CCATC | 3 cut(s) | 47, 723, 1049 |
| BciT130I | CCWGG | 2 cut(s) | 462, 576 |
| BcoDI | GTCTC | 2 cut(s) | 263, 1028 |
| BcuI | ACTAGT | 1 cut(s) | 977 |
| BfaI | CTAG | 4 cut(s) | 399, 755, 912, 978 |
| BglII | AGATCT | 1 cut(s) | 354 |
| BisI | GCNGC | 4 cut(s) | 434, 585, 804, 966 |
| BlsI | GCNGC | 4 cut(s) | 435, 586, 805, 967 |
| Bme1390I | CCNGG | 2 cut(s) | 462, 576 |
| Bme18I | GGWCC | 1 cut(s) | 849 |
| BmgT120I | GGNCC | 2 cut(s) | 849, 861 |
| BmiI | GGNNCC | 2 cut(s) | 175, 680 |
| BmrFI | CCNGG | 2 cut(s) | 462, 576 |
| BmsI | GCATC | 1 cut(s) | 933 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 556, 588 |
| Bpu14I | TTCGAA | 1 cut(s) | 366 |
| BsaJI | CCNNGG | 4 cut(s) | 177, 262, 343, 719 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 725, 858 |
| Bse1I | ACTGG | 3 cut(s) | 300, 716, 1018 |
| BseBI | CCWGG | 2 cut(s) | 462, 576 |
| BseDI | CCNNGG | 4 cut(s) | 177, 262, 343, 719 |
| BseGI | GGATG | 2 cut(s) | 484, 734 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 725, 858 |
| BseNI | ACTGG | 3 cut(s) | 300, 716, 1018 |
| BseXI | GCAGC | 4 cut(s) | 445, 571, 815, 977 |
| BseYI | CCCAGC | 1 cut(s) | 601 |
| BsgI | GTGCAG | 1 cut(s) | 570 |
| BshFI | GGCC | 2 cut(s) | 518, 862 |
| BshNI | GGYRCC | 1 cut(s) | 173 |
| BsiHKAI | GWGCWC | 1 cut(s) | 295 |
| BslFI | GGGAC | 2 cut(s) | 406, 548 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 725, 858 |
| BsmAI | GTCTC | 2 cut(s) | 263, 1028 |
| BsmBI | CGTCTC | 1 cut(s) | 263 |
| BsmFI | GGGAC | 2 cut(s) | 406, 548 |
| BsmI | GAATGC | 2 cut(s) | 431, 651 |
| BsnI | GGCC | 2 cut(s) | 518, 862 |
| Bsp119I | TTCGAA | 1 cut(s) | 366 |
| Bsp1286I | GDGCHC | 1 cut(s) | 295 |
| Bsp1407I | TGTACA | 1 cut(s) | 633 |
| Bsp143I | GATC | 2 cut(s) | 181, 354 |
| Bsp19I | CCATGG | 2 cut(s) | 262, 343 |
| BspACI | CCGC | 1 cut(s) | 212 |
| BspANI | GGCC | 2 cut(s) | 518, 862 |
| BspHI | TCATGA | 2 cut(s) | 376, 1044 |
| BspLI | GGNNCC | 2 cut(s) | 175, 680 |
| BspPI | GGATC | 1 cut(s) | 189 |
| BspT104I | TTCGAA | 1 cut(s) | 366 |
| BspT107I | GGYRCC | 1 cut(s) | 173 |
| BsrGI | TGTACA | 1 cut(s) | 633 |
| BsrI | ACTGG | 3 cut(s) | 300, 716, 1018 |
| BssECI | CCNNGG | 4 cut(s) | 177, 262, 343, 719 |
| BssMI | GATC | 2 cut(s) | 181, 354 |
| BssT1I | CCWWGG | 3 cut(s) | 262, 343, 719 |
| Bst2UI | CCWGG | 2 cut(s) | 462, 576 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 943 |
| BstAUI | TGTACA | 1 cut(s) | 633 |
| BstBI | TTCGAA | 1 cut(s) | 366 |
| BstC8I | GCNNGC | 1 cut(s) | 431 |
| BstDEI | CTNAG | 3 cut(s) | 167, 287, 358 |
| BstDSI | CCRYGG | 2 cut(s) | 262, 343 |
| BstF5I | GGATG | 2 cut(s) | 484, 734 |
| BstKTI | GATC | 2 cut(s) | 184, 357 |
| BstMAI | GTCTC | 2 cut(s) | 263, 1028 |
| BstMBI | GATC | 2 cut(s) | 181, 354 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 943 |
| BstNI | CCWGG | 2 cut(s) | 462, 576 |
| BstSCI | CCNGG | 2 cut(s) | 460, 574 |
| BstV1I | GCAGC | 4 cut(s) | 445, 571, 815, 977 |
| BstX2I | RGATCY | 1 cut(s) | 354 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 597 |
| BstYI | RGATCY | 1 cut(s) | 354 |
| BsuRI | GGCC | 2 cut(s) | 518, 862 |
| BtgI | CCRYGG | 2 cut(s) | 262, 343 |
| BtsCI | GGATG | 2 cut(s) | 484, 734 |
| BtsI | GCAGTG | 2 cut(s) | 162, 777 |
| BtsIMutI | CAGTG | 3 cut(s) | 162, 293, 777 |
| Cac8I | GCNNGC | 1 cut(s) | 431 |
| CciI | TCATGA | 2 cut(s) | 376, 1044 |
| Cfr13I | GGNCC | 2 cut(s) | 849, 861 |
| CseI | GACGC | 1 cut(s) | 1059 |
| Csp6I | GTAC | 3 cut(s) | 174, 222, 634 |
| CviAII | CATG | 7 cut(s) | 208, 242, 263, 344, 377, 961, 1045 |
| CviQI | GTAC | 3 cut(s) | 174, 222, 634 |
| DdeI | CTNAG | 3 cut(s) | 167, 287, 358 |
| DpnI | GATC | 2 cut(s) | 183, 356 |
| DpnII | GATC | 2 cut(s) | 181, 354 |
| EaeI | YGGCCR | 1 cut(s) | 516 |
| EciI | GGCGGA | 1 cut(s) | 227 |
| Eco130I | CCWWGG | 3 cut(s) | 262, 343, 719 |
| Eco47I | GGWCC | 1 cut(s) | 849 |
| EcoO109I | RGGNCCY | 1 cut(s) | 861 |
| EcoRII | CCWGG | 2 cut(s) | 460, 574 |
| EcoT14I | CCWWGG | 3 cut(s) | 262, 343, 719 |
| ErhI | CCWWGG | 3 cut(s) | 262, 343, 719 |
| Esp3I | CGTCTC | 1 cut(s) | 263 |
| FaeI | CATG | 7 cut(s) | 211, 245, 266, 347, 380, 964, 1048 |
| FaqI | GGGAC | 2 cut(s) | 406, 548 |
| FatI | CATG | 7 cut(s) | 207, 241, 262, 343, 376, 960, 1044 |
| FblI | GTMKAC | 1 cut(s) | 1073 |
| Fnu4HI | GCNGC | 4 cut(s) | 434, 585, 804, 966 |
| FokI | GGATG | 2 cut(s) | 491, 741 |
| Fsp4HI | GCNGC | 4 cut(s) | 434, 585, 804, 966 |
| FspBI | CTAG | 4 cut(s) | 399, 755, 912, 978 |
| GluI | GCNGC | 4 cut(s) | 434, 585, 804, 966 |
| GsaI | CCCAGC | 1 cut(s) | 605 |
| HaeIII | GGCC | 2 cut(s) | 518, 862 |
| HgaI | GACGC | 1 cut(s) | 1059 |
| Hin1II | CATG | 7 cut(s) | 211, 245, 266, 347, 380, 964, 1048 |
| HincII | GTYRAC | 1 cut(s) | 247 |
| HindII | GTYRAC | 1 cut(s) | 247 |
| HindIII | AAGCTT | 1 cut(s) | 371 |
| HinfI | GANTC | 5 cut(s) | 235, 363, 907, 995, 1036 |
| HphI | GGTGA | 3 cut(s) | 76, 761, 892 |
| Hpy166II | GTNNAC | 7 cut(s) | 48, 224, 247, 445, 634, 752, 1074 |
| Hpy188I | TCNGA | 3 cut(s) | 66, 181, 853 |
| Hpy188III | TCNNGA | 4 cut(s) | 102, 377, 658, 1045 |
| Hpy8I | GTNNAC | 7 cut(s) | 48, 224, 247, 445, 634, 752, 1074 |
| HpyAV | CCTTC | 5 cut(s) | 154, 669, 887, 927, 994 |
| HpyCH4V | TGCA | 5 cut(s) | 71, 386, 418, 528, 587 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 943 |
| HpyF3I | CTNAG | 3 cut(s) | 167, 287, 358 |
| Hsp92II | CATG | 7 cut(s) | 211, 245, 266, 347, 380, 964, 1048 |
| KpnI | GGTACC | 1 cut(s) | 177 |
| Kzo9I | GATC | 2 cut(s) | 181, 354 |
| Lsp1109I | GCAGC | 4 cut(s) | 445, 571, 815, 977 |
| LweI | GCATC | 1 cut(s) | 933 |
| MaeI | CTAG | 4 cut(s) | 399, 755, 912, 978 |
| MalI | GATC | 2 cut(s) | 183, 356 |
| MboI | GATC | 2 cut(s) | 181, 354 |
| MboII | GAAGA | 4 cut(s) | 434, 481, 684, 745 |
| MflI | RGATCY | 1 cut(s) | 354 |
| MhlI | GDGCHC | 1 cut(s) | 295 |
| MlsI | TGGCCA | 1 cut(s) | 518 |
| MluCI | AATT | 8 cut(s) | 7, 60, 192, 203, 381, 484, 899, 921 |
| MluNI | TGGCCA | 1 cut(s) | 518 |
| MlyI | GAGTC | 1 cut(s) | 1030 |
| MmeI | TCCRAC | 1 cut(s) | 870 |
| MnlI | CCTC | 8 cut(s) | 98, 187, 395, 558, 778, 793, 852, 1009 |
| Mox20I | TGGCCA | 1 cut(s) | 518 |
| MroXI | GAANNNNTTC | 2 cut(s) | 372, 488 |
| MscI | TGGCCA | 1 cut(s) | 518 |
| MseI | TTAA | 5 cut(s) | 690, 818, 866, 924, 1078 |
| MslI | CAYNNNNRTG | 1 cut(s) | 624 |
| Msp20I | TGGCCA | 1 cut(s) | 518 |
| MspR9I | CCNGG | 2 cut(s) | 462, 576 |
| Mva1269I | GAATGC | 2 cut(s) | 431, 651 |
| MvaI | CCWGG | 2 cut(s) | 462, 576 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 943 |
| NcoI | CCATGG | 2 cut(s) | 262, 343 |
| NdeII | GATC | 2 cut(s) | 181, 354 |
| NlaIII | CATG | 7 cut(s) | 211, 245, 266, 347, 380, 964, 1048 |
| NlaIV | GGNNCC | 2 cut(s) | 175, 680 |
| NspV | TTCGAA | 1 cut(s) | 366 |
| OliI | CACNNNNGTG | 1 cut(s) | 624 |
| PagI | TCATGA | 2 cut(s) | 376, 1044 |
| PctI | GAATGC | 2 cut(s) | 431, 651 |
| PdmI | GAANNNNTTC | 2 cut(s) | 372, 488 |
| PfeI | GAWTC | 4 cut(s) | 235, 363, 907, 995 |
| PkrI | GCNGC | 4 cut(s) | 435, 586, 805, 967 |
| PleI | GAGTC | 1 cut(s) | 1030 |
| PpsI | GAGTC | 1 cut(s) | 1030 |
| PshBI | ATTAAT | 1 cut(s) | 924 |
| PsiI | TTATAA | 1 cut(s) | 123 |
| Psp6I | CCWGG | 2 cut(s) | 460, 574 |
| PspFI | CCCAGC | 1 cut(s) | 601 |
| PspGI | CCWGG | 2 cut(s) | 460, 574 |
| PspN4I | GGNNCC | 2 cut(s) | 175, 680 |
| PspPI | GGNCC | 2 cut(s) | 849, 861 |
| PsuI | RGATCY | 1 cut(s) | 354 |
| RsaI | GTAC | 3 cut(s) | 175, 223, 635 |
| RsaNI | GTAC | 3 cut(s) | 174, 222, 634 |
| RseI | CAYNNNNRTG | 1 cut(s) | 624 |
| SaqAI | TTAA | 5 cut(s) | 690, 818, 866, 924, 1078 |
| SatI | GCNGC | 4 cut(s) | 434, 585, 804, 966 |
| Sau3AI | GATC | 2 cut(s) | 181, 354 |
| Sau96I | GGNCC | 2 cut(s) | 849, 861 |
| SchI | GAGTC | 1 cut(s) | 1030 |
| ScrFI | CCNGG | 2 cut(s) | 462, 576 |
| SduI | GDGCHC | 1 cut(s) | 295 |
| SfaNI | GCATC | 1 cut(s) | 933 |
| SfuI | TTCGAA | 1 cut(s) | 366 |
| SinI | GGWCC | 1 cut(s) | 849 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 624 |
| SpeI | ACTAGT | 1 cut(s) | 977 |
| Sse9I | AATT | 8 cut(s) | 7, 60, 192, 203, 381, 484, 899, 921 |
| SsiI | CCGC | 1 cut(s) | 212 |
| SspI | AATATT | 1 cut(s) | 469 |
| SspMI | CTAG | 4 cut(s) | 399, 755, 912, 978 |
| StyD4I | CCNGG | 2 cut(s) | 460, 574 |
| StyI | CCWWGG | 3 cut(s) | 262, 343, 719 |
| TaqI | TCGA | 3 cut(s) | 366, 657, 987 |
| TasI | AATT | 8 cut(s) | 7, 60, 192, 203, 381, 484, 899, 921 |
| TatI | WGTACW | 2 cut(s) | 221, 633 |
| TfiI | GAWTC | 4 cut(s) | 235, 363, 907, 995 |
| Tru1I | TTAA | 5 cut(s) | 690, 818, 866, 924, 1078 |
| Tru9I | TTAA | 5 cut(s) | 690, 818, 866, 924, 1078 |
| TscAI | CASTG | 3 cut(s) | 162, 300, 777 |
| TseI | GCWGC | 4 cut(s) | 433, 584, 803, 965 |
| TspDTI | ATGAA | 5 cut(s) | 196, 227, 365, 393, 1061 |
| TspGWI | ACGGA | 1 cut(s) | 1007 |
| TspRI | CASTG | 3 cut(s) | 162, 300, 777 |
| VpaK11BI | GGWCC | 1 cut(s) | 849 |
| VspI | ATTAAT | 1 cut(s) | 924 |
| XapI | RAATTY | 3 cut(s) | 60, 484, 899 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 726 |
| XmiI | GTMKAC | 1 cut(s) | 1073 |
| XmnI | GAANNNNTTC | 2 cut(s) | 372, 488 |
| XspI | CTAG | 4 cut(s) | 399, 755, 912, 978 |