Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 270 |
| Acc65I | GGTACC | 1 cut(s) | 320 |
| AccB1I | GGYRCC | 1 cut(s) | 320 |
| AccI | GTMKAC | 1 cut(s) | 5 |
| AccIII | TCCGGA | 1 cut(s) | 841 |
| AciI | CCGC | 1 cut(s) | 356 |
| AclWI | GGATC | 2 cut(s) | 101, 119 |
| AcoI | YGGCCR | 1 cut(s) | 660 |
| AcsI | RAATTY | 1 cut(s) | 278 |
| AcuI | CTGAAG | 4 cut(s) | 30, 194, 415, 1061 |
| AfaI | GTAC | 3 cut(s) | 114, 322, 779 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 375, 708, 869, 1002 |
| AgsI | TTSAA | 5 cut(s) | 341, 673, 887, 1065, 1166 |
| AhlI | ACTAGT | 1 cut(s) | 1121 |
| AjnI | CCWGG | 2 cut(s) | 718, 1090 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 516, 548 |
| AleI | CACNNNNGTG | 1 cut(s) | 768 |
| Alw26I | GTCTC | 2 cut(s) | 46, 418 |
| AlwI | GGATC | 2 cut(s) | 101, 119 |
| Ama87I | CYCGRG | 1 cut(s) | 995 |
| Aor13HI | TCCGGA | 1 cut(s) | 841 |
| AoxI | GGCC | 4 cut(s) | 182, 660, 992, 1004 |
| ApeKI | GCWGC | 4 cut(s) | 96, 577, 728, 745 |
| ApoI | RAATTY | 1 cut(s) | 278 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 516, 612 |
| Asp718I | GGTACC | 1 cut(s) | 320 |
| AspS9I | GGNCC | 3 cut(s) | 183, 310, 993 |
| AsuC2I | CCSGG | 1 cut(s) | 703 |
| AsuHPI | GGTGA | 2 cut(s) | 223, 1036 |
| AsuII | TTCGAA | 1 cut(s) | 510 |
| AvaI | CYCGRG | 1 cut(s) | 995 |
| AvaII | GGWCC | 1 cut(s) | 310 |
| BaeGI | GKGCMC | 1 cut(s) | 755 |
| BalI | TGGCCA | 1 cut(s) | 662 |
| BanI | GGYRCC | 1 cut(s) | 320 |
| BbsI | GAAGAC | 1 cut(s) | 1120 |
| BbvI | GCAGC | 4 cut(s) | 108, 589, 715, 732 |
| BccI | CCATC | 1 cut(s) | 867 |
| BciT130I | CCWGG | 2 cut(s) | 720, 1092 |
| BcnI | CCSGG | 1 cut(s) | 703 |
| BcoDI | GTCTC | 2 cut(s) | 46, 418 |
| BcuI | ACTAGT | 1 cut(s) | 1121 |
| BfaI | CTAG | 6 cut(s) | 543, 827, 899, 914, 1056, 1122 |
| BfmI | CTRYAG | 1 cut(s) | 746 |
| BisI | GCNGC | 4 cut(s) | 97, 578, 729, 746 |
| BlsI | GCNGC | 4 cut(s) | 98, 579, 730, 747 |
| BmcAI | AGTACT | 1 cut(s) | 114 |
| Bme1390I | CCNGG | 3 cut(s) | 703, 720, 1092 |
| Bme18I | GGWCC | 1 cut(s) | 310 |
| BmeT110I | CYCGRG | 1 cut(s) | 995 |
| BmgT120I | GGNCC | 3 cut(s) | 183, 310, 993 |
| BmiI | GGNNCC | 3 cut(s) | 292, 322, 824 |
| BmrFI | CCNGG | 3 cut(s) | 703, 720, 1092 |
| BpiI | GAAGAC | 1 cut(s) | 1120 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 700, 732 |
| BpmI | CTGGAG | 1 cut(s) | 912 |
| Bpu14I | TTCGAA | 1 cut(s) | 510 |
| BpuMI | CCSGG | 1 cut(s) | 703 |
| BsaBI | GATNNNNATC | 1 cut(s) | 56 |
| BsaJI | CCNNGG | 4 cut(s) | 324, 370, 487, 863 |
| BsaWI | WCCGGW | 1 cut(s) | 841 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 375, 708, 869, 1002 |
| Bse1I | ACTGG | 3 cut(s) | 110, 860, 1162 |
| Bse8I | GATNNNNATC | 1 cut(s) | 56 |
| BseAI | TCCGGA | 1 cut(s) | 841 |
| BseBI | CCWGG | 2 cut(s) | 720, 1092 |
| BseDI | CCNNGG | 4 cut(s) | 324, 370, 487, 863 |
| BseGI | GGATG | 3 cut(s) | 628, 878, 1175 |
| BseJI | GATNNNNATC | 1 cut(s) | 56 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 375, 708, 869, 1002 |
| BseNI | ACTGG | 3 cut(s) | 110, 860, 1162 |
| BseRI | GAGGAG | 1 cut(s) | 944 |
| BseSI | GKGCMC | 1 cut(s) | 755 |
| BseXI | GCAGC | 4 cut(s) | 108, 589, 715, 732 |
| BseYI | CCCAGC | 1 cut(s) | 241 |
| BsgI | GTGCAG | 1 cut(s) | 714 |
| BshFI | GGCC | 4 cut(s) | 184, 662, 994, 1006 |
| BshNI | GGYRCC | 1 cut(s) | 320 |
| BsiHKCI | CYCGRG | 1 cut(s) | 995 |
| BsiSI | CCGG | 2 cut(s) | 702, 842 |
| BslFI | GGGAC | 1 cut(s) | 550 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 375, 708, 869, 1002 |
| BsmAI | GTCTC | 2 cut(s) | 46, 418 |
| BsmFI | GGGAC | 1 cut(s) | 550 |
| BsmI | GAATGC | 2 cut(s) | 575, 795 |
| BsnI | GGCC | 4 cut(s) | 184, 662, 994, 1006 |
| BsoBI | CYCGRG | 1 cut(s) | 995 |
| Bsp119I | TTCGAA | 1 cut(s) | 510 |
| Bsp1286I | GDGCHC | 1 cut(s) | 755 |
| Bsp13I | TCCGGA | 1 cut(s) | 841 |
| Bsp1407I | TGTACA | 1 cut(s) | 777 |
| Bsp143I | GATC | 3 cut(s) | 106, 124, 804 |
| Bsp19I | CCATGG | 1 cut(s) | 487 |
| BspACI | CCGC | 1 cut(s) | 356 |
| BspANI | GGCC | 4 cut(s) | 184, 662, 994, 1006 |
| BspEI | TCCGGA | 1 cut(s) | 841 |
| BspHI | TCATGA | 1 cut(s) | 520 |
| BspLI | GGNNCC | 3 cut(s) | 292, 322, 824 |
| BspMAI | CTGCAG | 1 cut(s) | 750 |
| BspPI | GGATC | 2 cut(s) | 101, 119 |
| BspT104I | TTCGAA | 1 cut(s) | 510 |
| BspT107I | GGYRCC | 1 cut(s) | 320 |
| BsrGI | TGTACA | 1 cut(s) | 777 |
| BsrI | ACTGG | 3 cut(s) | 110, 860, 1162 |
| BssECI | CCNNGG | 4 cut(s) | 324, 370, 487, 863 |
| BssMI | GATC | 3 cut(s) | 106, 124, 804 |
| BssT1I | CCWWGG | 3 cut(s) | 370, 487, 863 |
| Bst2UI | CCWGG | 2 cut(s) | 720, 1092 |
| Bst4CI | ACNGT | 1 cut(s) | 22 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1087 |
| BstAUI | TGTACA | 1 cut(s) | 777 |
| BstBI | TTCGAA | 1 cut(s) | 510 |
| BstC8I | GCNNGC | 1 cut(s) | 575 |
| BstDSI | CCRYGG | 1 cut(s) | 487 |
| BstF5I | GGATG | 3 cut(s) | 628, 878, 1175 |
| BstKTI | GATC | 3 cut(s) | 109, 127, 807 |
| BstMAI | GTCTC | 2 cut(s) | 46, 418 |
| BstMBI | GATC | 3 cut(s) | 106, 124, 804 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1087 |
| BstNI | CCWGG | 2 cut(s) | 720, 1092 |
| BstSCI | CCNGG | 3 cut(s) | 701, 718, 1090 |
| BstSFI | CTRYAG | 1 cut(s) | 746 |
| BstSLI | GKGCMC | 1 cut(s) | 755 |
| BstV1I | GCAGC | 4 cut(s) | 108, 589, 715, 732 |
| BstV2I | GAAGAC | 1 cut(s) | 1120 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 241, 741 |
| BsuRI | GGCC | 4 cut(s) | 184, 662, 994, 1006 |
| BtgI | CCRYGG | 1 cut(s) | 487 |
| BtsCI | GGATG | 3 cut(s) | 628, 878, 1175 |
| BtsI | GCAGTG | 2 cut(s) | 309, 755 |
| BtsIMutI | CAGTG | 2 cut(s) | 309, 755 |
| Cac8I | GCNNGC | 1 cut(s) | 575 |
| CciI | TCATGA | 1 cut(s) | 520 |
| Cfr13I | GGNCC | 3 cut(s) | 183, 310, 993 |
| Csp6I | GTAC | 3 cut(s) | 113, 321, 778 |
| CviAII | CATG | 6 cut(s) | 122, 187, 352, 488, 521, 1105 |
| CviQI | GTAC | 3 cut(s) | 113, 321, 778 |
| DpnI | GATC | 3 cut(s) | 108, 126, 806 |
| DpnII | GATC | 3 cut(s) | 106, 124, 804 |
| EaeI | YGGCCR | 1 cut(s) | 660 |
| EciI | GGCGGA | 1 cut(s) | 371 |
| Eco130I | CCWWGG | 3 cut(s) | 370, 487, 863 |
| Eco147I | AGGCCT | 1 cut(s) | 1006 |
| Eco47I | GGWCC | 1 cut(s) | 310 |
| Eco57I | CTGAAG | 4 cut(s) | 30, 194, 415, 1061 |
| Eco88I | CYCGRG | 1 cut(s) | 995 |
| EcoO109I | RGGNCCY | 1 cut(s) | 310 |
| EcoRII | CCWGG | 2 cut(s) | 718, 1090 |
| EcoT14I | CCWWGG | 3 cut(s) | 370, 487, 863 |
| ErhI | CCWWGG | 3 cut(s) | 370, 487, 863 |
| FaeI | CATG | 6 cut(s) | 125, 190, 355, 491, 524, 1108 |
| FaqI | GGGAC | 1 cut(s) | 550 |
| FatI | CATG | 6 cut(s) | 121, 186, 351, 487, 520, 1104 |
| FblI | GTMKAC | 1 cut(s) | 5 |
| Fnu4HI | GCNGC | 4 cut(s) | 97, 578, 729, 746 |
| FokI | GGATG | 2 cut(s) | 635, 885 |
| Fsp4HI | GCNGC | 4 cut(s) | 97, 578, 729, 746 |
| FspBI | CTAG | 6 cut(s) | 543, 827, 899, 914, 1056, 1122 |
| GluI | GCNGC | 4 cut(s) | 97, 578, 729, 746 |
| GsaI | CCCAGC | 1 cut(s) | 245 |
| GsuI | CTGGAG | 1 cut(s) | 912 |
| HaeIII | GGCC | 4 cut(s) | 184, 662, 994, 1006 |
| HapII | CCGG | 2 cut(s) | 702, 842 |
| Hin1II | CATG | 6 cut(s) | 125, 190, 355, 491, 524, 1108 |
| HincII | GTYRAC | 1 cut(s) | 6 |
| HindII | GTYRAC | 1 cut(s) | 6 |
| HindIII | AAGCTT | 2 cut(s) | 130, 515 |
| HinfI | GANTC | 5 cut(s) | 379, 507, 685, 1051, 1139 |
| HpaII | CCGG | 2 cut(s) | 702, 842 |
| HphI | GGTGA | 2 cut(s) | 223, 1036 |
| Hpy166II | GTNNAC | 6 cut(s) | 6, 195, 467, 589, 628, 778 |
| Hpy188I | TCNGA | 4 cut(s) | 103, 213, 328, 434 |
| Hpy188III | TCNNGA | 6 cut(s) | 86, 249, 521, 842, 851, 1146 |
| Hpy8I | GTNNAC | 6 cut(s) | 6, 195, 467, 589, 628, 778 |
| HpyAV | CCTTC | 4 cut(s) | 301, 813, 1071, 1160 |
| HpyCH4III | ACNGT | 1 cut(s) | 22 |
| HpyCH4IV | ACGT | 1 cut(s) | 91 |
| HpyCH4V | TGCA | 6 cut(s) | 218, 530, 562, 731, 748, 1036 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1087 |
| HpySE526I | ACGT | 1 cut(s) | 91 |
| Hsp92II | CATG | 6 cut(s) | 125, 190, 355, 491, 524, 1108 |
| Kpn2I | TCCGGA | 1 cut(s) | 841 |
| KpnI | GGTACC | 1 cut(s) | 324 |
| Kzo9I | GATC | 3 cut(s) | 106, 124, 804 |
| Lsp1109I | GCAGC | 4 cut(s) | 108, 589, 715, 732 |
| MaeI | CTAG | 6 cut(s) | 543, 827, 899, 914, 1056, 1122 |
| MaeII | ACGT | 1 cut(s) | 91 |
| MaeIII | GTNAC | 1 cut(s) | 16 |
| MalI | GATC | 3 cut(s) | 108, 126, 806 |
| MboI | GATC | 3 cut(s) | 106, 124, 804 |
| MboII | GAAGA | 6 cut(s) | 353, 578, 828, 830, 889, 1120 |
| MfeI | CAATTG | 1 cut(s) | 438 |
| MhlI | GDGCHC | 1 cut(s) | 755 |
| MlsI | TGGCCA | 1 cut(s) | 662 |
| MluCI | AATT | 8 cut(s) | 154, 278, 336, 347, 438, 525, 1044, 1065 |
| MluNI | TGGCCA | 1 cut(s) | 662 |
| MlyI | GAGTC | 1 cut(s) | 679 |
| Mox20I | TGGCCA | 1 cut(s) | 662 |
| MroI | TCCGGA | 1 cut(s) | 841 |
| MroXI | GAANNNNTTC | 2 cut(s) | 516, 612 |
| MscI | TGGCCA | 1 cut(s) | 662 |
| MseI | TTAA | 3 cut(s) | 962, 1010, 1068 |
| MslI | CAYNNNNRTG | 1 cut(s) | 768 |
| Msp20I | TGGCCA | 1 cut(s) | 662 |
| MspI | CCGG | 2 cut(s) | 702, 842 |
| MspR9I | CCNGG | 3 cut(s) | 703, 720, 1092 |
| MunI | CAATTG | 1 cut(s) | 438 |
| Mva1269I | GAATGC | 2 cut(s) | 575, 795 |
| MvaI | CCWGG | 2 cut(s) | 720, 1092 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1087 |
| NciI | CCSGG | 1 cut(s) | 703 |
| NcoI | CCATGG | 1 cut(s) | 487 |
| NdeII | GATC | 3 cut(s) | 106, 124, 804 |
| NlaIII | CATG | 6 cut(s) | 125, 190, 355, 491, 524, 1108 |
| NlaIV | GGNNCC | 3 cut(s) | 292, 322, 824 |
| NspV | TTCGAA | 1 cut(s) | 510 |
| OliI | CACNNNNGTG | 1 cut(s) | 768 |
| PagI | TCATGA | 1 cut(s) | 520 |
| PceI | AGGCCT | 1 cut(s) | 1006 |
| PctI | GAATGC | 2 cut(s) | 575, 795 |
| PdmI | GAANNNNTTC | 2 cut(s) | 516, 612 |
| PfeI | GAWTC | 4 cut(s) | 379, 507, 1051, 1139 |
| PkrI | GCNGC | 4 cut(s) | 98, 579, 730, 747 |
| PleI | GAGTC | 1 cut(s) | 679 |
| PpsI | GAGTC | 1 cut(s) | 679 |
| PpuMI | RGGWCCY | 1 cut(s) | 310 |
| PsiI | TTATAA | 1 cut(s) | 270 |
| Psp5II | RGGWCCY | 1 cut(s) | 310 |
| Psp6I | CCWGG | 2 cut(s) | 718, 1090 |
| PspFI | CCCAGC | 1 cut(s) | 241 |
| PspGI | CCWGG | 2 cut(s) | 718, 1090 |
| PspN4I | GGNNCC | 3 cut(s) | 292, 322, 824 |
| PspPI | GGNCC | 3 cut(s) | 183, 310, 993 |
| PspPPI | RGGWCCY | 1 cut(s) | 310 |
| PstI | CTGCAG | 1 cut(s) | 750 |
| RsaI | GTAC | 3 cut(s) | 114, 322, 779 |
| RsaNI | GTAC | 3 cut(s) | 113, 321, 778 |
| RseI | CAYNNNNRTG | 1 cut(s) | 768 |
| SalI | GTCGAC | 1 cut(s) | 4 |
| SaqAI | TTAA | 3 cut(s) | 962, 1010, 1068 |
| SatI | GCNGC | 4 cut(s) | 97, 578, 729, 746 |
| Sau3AI | GATC | 3 cut(s) | 106, 124, 804 |
| Sau96I | GGNCC | 3 cut(s) | 183, 310, 993 |
| ScaI | AGTACT | 1 cut(s) | 114 |
| SchI | GAGTC | 1 cut(s) | 679 |
| ScrFI | CCNGG | 3 cut(s) | 703, 720, 1092 |
| SduI | GDGCHC | 1 cut(s) | 755 |
| SfcI | CTRYAG | 1 cut(s) | 746 |
| SfuI | TTCGAA | 1 cut(s) | 510 |
| SinI | GGWCC | 1 cut(s) | 310 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 768 |
| SpeI | ACTAGT | 1 cut(s) | 1121 |
| Sse9I | AATT | 8 cut(s) | 154, 278, 336, 347, 438, 525, 1044, 1065 |
| SseBI | AGGCCT | 1 cut(s) | 1006 |
| SsiI | CCGC | 1 cut(s) | 356 |
| SspI | AATATT | 1 cut(s) | 613 |
| SspMI | CTAG | 6 cut(s) | 543, 827, 899, 914, 1056, 1122 |
| StuI | AGGCCT | 1 cut(s) | 1006 |
| StyD4I | CCNGG | 3 cut(s) | 701, 718, 1090 |
| StyI | CCWWGG | 3 cut(s) | 370, 487, 863 |
| TaaI | ACNGT | 1 cut(s) | 22 |
| TaiI | ACGT | 1 cut(s) | 94 |
| TaqI | TCGA | 5 cut(s) | 5, 137, 510, 1131, 1147 |
| TasI | AATT | 8 cut(s) | 154, 278, 336, 347, 438, 525, 1044, 1065 |
| TatI | WGTACW | 2 cut(s) | 112, 777 |
| TfiI | GAWTC | 4 cut(s) | 379, 507, 1051, 1139 |
| Tru1I | TTAA | 3 cut(s) | 962, 1010, 1068 |
| Tru9I | TTAA | 3 cut(s) | 962, 1010, 1068 |
| TscAI | CASTG | 2 cut(s) | 309, 755 |
| TseI | GCWGC | 4 cut(s) | 96, 577, 728, 745 |
| TspDTI | ATGAA | 4 cut(s) | 340, 371, 509, 537 |
| TspGWI | ACGGA | 1 cut(s) | 1151 |
| TspRI | CASTG | 2 cut(s) | 309, 755 |
| VpaK11BI | GGWCC | 1 cut(s) | 310 |
| XapI | RAATTY | 1 cut(s) | 278 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 870 |
| XmiI | GTMKAC | 1 cut(s) | 5 |
| XmnI | GAANNNNTTC | 2 cut(s) | 516, 612 |
| XspI | CTAG | 6 cut(s) | 543, 827, 899, 914, 1056, 1122 |
| ZrmI | AGTACT | 1 cut(s) | 114 |