Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 272, 381 |
| Acc65I | GGTACC | 1 cut(s) | 1259 |
| AccB1I | GGYRCC | 1 cut(s) | 1259 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1256 |
| AccBSI | CCGCTC | 1 cut(s) | 53 |
| AccI | GTMKAC | 1 cut(s) | 5 |
| AccII | CGCG | 1 cut(s) | 153 |
| AciI | CCGC | 4 cut(s) | 53, 410, 467, 862 |
| AclWI | GGATC | 2 cut(s) | 583, 671 |
| AcoI | YGGCCR | 1 cut(s) | 774 |
| AfaI | GTAC | 5 cut(s) | 60, 478, 563, 590, 1261 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 982, 1256 |
| AgsI | TTSAA | 5 cut(s) | 626, 1001, 1125, 1179, 1352 |
| AleI | CACNNNNGTG | 1 cut(s) | 882 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 728, 760 |
| AluBI | AGCT | 5 cut(s) | 761, 781, 897, 1021, 1085 |
| AluI | AGCT | 5 cut(s) | 761, 781, 897, 1021, 1085 |
| Alw26I | GTCTC | 1 cut(s) | 960 |
| AlwI | GGATC | 2 cut(s) | 583, 671 |
| Ama87I | CYCGRG | 1 cut(s) | 976 |
| AoxI | GGCC | 3 cut(s) | 355, 774, 1118 |
| ApeKI | GCWGC | 4 cut(s) | 842, 859, 1223, 1266 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 630, 726 |
| Asp718I | GGTACC | 1 cut(s) | 1259 |
| AspLEI | GCGC | 2 cut(s) | 155, 180 |
| AspS9I | GGNCC | 1 cut(s) | 508 |
| AsuC2I | CCSGG | 3 cut(s) | 200, 834, 1005 |
| AsuHPI | GGTGA | 2 cut(s) | 334, 1150 |
| AvaI | CYCGRG | 1 cut(s) | 976 |
| AvaII | GGWCC | 1 cut(s) | 508 |
| BaeGI | GKGCMC | 1 cut(s) | 253 |
| BalI | TGGCCA | 1 cut(s) | 776 |
| BanI | GGYRCC | 1 cut(s) | 1259 |
| BbsI | GAAGAC | 1 cut(s) | 687 |
| BbvI | GCAGC | 4 cut(s) | 829, 871, 1210, 1278 |
| BccI | CCATC | 1 cut(s) | 1018 |
| BceAI | ACGGC | 1 cut(s) | 1298 |
| BcnI | CCSGG | 3 cut(s) | 200, 834, 1005 |
| BcoDI | GTCTC | 1 cut(s) | 960 |
| BfaI | CTAG | 2 cut(s) | 1170, 1366 |
| BisI | GCNGC | 5 cut(s) | 411, 843, 860, 1224, 1267 |
| BlsI | GCNGC | 5 cut(s) | 412, 844, 861, 1225, 1268 |
| BmcAI | AGTACT | 1 cut(s) | 590 |
| Bme1390I | CCNGG | 3 cut(s) | 200, 834, 1005 |
| Bme18I | GGWCC | 1 cut(s) | 508 |
| BmeT110I | CYCGRG | 1 cut(s) | 976 |
| BmgT120I | GGNCC | 1 cut(s) | 508 |
| BmiI | GGNNCC | 3 cut(s) | 403, 866, 1261 |
| BmrFI | CCNGG | 3 cut(s) | 200, 834, 1005 |
| BmsI | GCATC | 1 cut(s) | 727 |
| BpiI | GAAGAC | 1 cut(s) | 687 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 814, 846 |
| BpmI | CTGGAG | 1 cut(s) | 222 |
| BpuMI | CCSGG | 3 cut(s) | 200, 834, 1005 |
| BsaJI | CCNNGG | 3 cut(s) | 198, 358, 977 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 42, 72 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 982, 1256 |
| Bse1I | ACTGG | 2 cut(s) | 1099, 1261 |
| Bse3DI | GCAATG | 3 cut(s) | 65, 403, 1164 |
| BseDI | CCNNGG | 3 cut(s) | 198, 358, 977 |
| BseGI | GGATG | 1 cut(s) | 742 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 982, 1256 |
| BseMI | GCAATG | 3 cut(s) | 65, 403, 1164 |
| BseMII | CTCAG | 1 cut(s) | 556 |
| BseNI | ACTGG | 2 cut(s) | 1099, 1261 |
| BseRI | GAGGAG | 2 cut(s) | 375, 1058 |
| BseSI | GKGCMC | 1 cut(s) | 253 |
| BseXI | GCAGC | 4 cut(s) | 829, 871, 1210, 1278 |
| BseYI | CCCAGC | 1 cut(s) | 406 |
| BsgI | GTGCAG | 3 cut(s) | 206, 393, 1209 |
| Bsh1236I | CGCG | 1 cut(s) | 153 |
| Bsh1285I | CGRYCG | 1 cut(s) | 208 |
| BshFI | GGCC | 3 cut(s) | 357, 776, 1120 |
| BshNI | GGYRCC | 1 cut(s) | 1259 |
| BsiEI | CGRYCG | 1 cut(s) | 208 |
| BsiHKCI | CYCGRG | 1 cut(s) | 976 |
| BsiSI | CCGG | 3 cut(s) | 200, 833, 1005 |
| BslFI | GGGAC | 3 cut(s) | 664, 806, 841 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 982, 1256 |
| BsmAI | GTCTC | 1 cut(s) | 960 |
| BsmFI | GGGAC | 3 cut(s) | 664, 806, 841 |
| BsmI | GAATGC | 1 cut(s) | 909 |
| BsnI | GGCC | 3 cut(s) | 357, 776, 1120 |
| BsoBI | CYCGRG | 1 cut(s) | 976 |
| Bsp1286I | GDGCHC | 1 cut(s) | 253 |
| Bsp143I | GATC | 5 cut(s) | 189, 205, 228, 575, 663 |
| BspACI | CCGC | 4 cut(s) | 53, 410, 467, 862 |
| BspANI | GGCC | 3 cut(s) | 357, 776, 1120 |
| BspCNI | CTCAG | 1 cut(s) | 555 |
| BspFNI | CGCG | 1 cut(s) | 153 |
| BspHI | TCATGA | 2 cut(s) | 634, 1332 |
| BspLI | GGNNCC | 3 cut(s) | 403, 866, 1261 |
| BspPI | GGATC | 2 cut(s) | 583, 671 |
| BspT107I | GGYRCC | 1 cut(s) | 1259 |
| BsrBI | CCGCTC | 1 cut(s) | 53 |
| BsrDI | GCAATG | 3 cut(s) | 65, 403, 1164 |
| BsrI | ACTGG | 2 cut(s) | 1099, 1261 |
| BssECI | CCNNGG | 3 cut(s) | 198, 358, 977 |
| BssMI | GATC | 5 cut(s) | 189, 205, 228, 575, 663 |
| Bst4CI | ACNGT | 3 cut(s) | 610, 971, 1297 |
| BstDEI | CTNAG | 4 cut(s) | 103, 181, 542, 616 |
| BstF5I | GGATG | 1 cut(s) | 742 |
| BstFNI | CGCG | 1 cut(s) | 153 |
| BstHHI | GCGC | 2 cut(s) | 155, 180 |
| BstKTI | GATC | 5 cut(s) | 192, 208, 231, 578, 666 |
| BstMAI | GTCTC | 1 cut(s) | 960 |
| BstMBI | GATC | 5 cut(s) | 189, 205, 228, 575, 663 |
| BstMCI | CGRYCG | 1 cut(s) | 208 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 125, 184 |
| BstSCI | CCNGG | 3 cut(s) | 198, 832, 1003 |
| BstSLI | GKGCMC | 1 cut(s) | 253 |
| BstUI | CGCG | 1 cut(s) | 153 |
| BstV1I | GCAGC | 4 cut(s) | 829, 871, 1210, 1278 |
| BstV2I | GAAGAC | 1 cut(s) | 687 |
| BsuRI | GGCC | 3 cut(s) | 357, 776, 1120 |
| BtsCI | GGATG | 1 cut(s) | 742 |
| BtsI | GCAGTG | 2 cut(s) | 135, 420 |
| BtsIMutI | CAGTG | 3 cut(s) | 135, 420, 615 |
| CciI | TCATGA | 2 cut(s) | 634, 1332 |
| CfoI | GCGC | 2 cut(s) | 155, 180 |
| Cfr13I | GGNCC | 1 cut(s) | 508 |
| CseI | GACGC | 2 cut(s) | 220, 1347 |
| Csp6I | GTAC | 5 cut(s) | 59, 477, 562, 589, 1260 |
| CviAII | CATG | 6 cut(s) | 428, 565, 635, 677, 811, 1333 |
| CviQI | GTAC | 5 cut(s) | 59, 477, 562, 589, 1260 |
| DdeI | CTNAG | 4 cut(s) | 103, 181, 542, 616 |
| DpnI | GATC | 5 cut(s) | 191, 207, 230, 577, 665 |
| DpnII | GATC | 5 cut(s) | 189, 205, 228, 575, 663 |
| EaeI | YGGCCR | 1 cut(s) | 774 |
| EciI | GGCGGA | 1 cut(s) | 482 |
| Eco147I | AGGCCT | 1 cut(s) | 1120 |
| Eco32I | GATATC | 1 cut(s) | 946 |
| Eco47I | GGWCC | 1 cut(s) | 508 |
| Eco88I | CYCGRG | 1 cut(s) | 976 |
| EcoRV | GATATC | 1 cut(s) | 946 |
| FaeI | CATG | 6 cut(s) | 431, 568, 638, 680, 814, 1336 |
| FaqI | GGGAC | 3 cut(s) | 664, 806, 841 |
| FatI | CATG | 6 cut(s) | 427, 564, 634, 676, 810, 1332 |
| FauI | CCCGC | 1 cut(s) | 46 |
| FblI | GTMKAC | 1 cut(s) | 5 |
| Fnu4HI | GCNGC | 5 cut(s) | 411, 843, 860, 1224, 1267 |
| FokI | GGATG | 1 cut(s) | 749 |
| Fsp4HI | GCNGC | 5 cut(s) | 411, 843, 860, 1224, 1267 |
| FspBI | CTAG | 2 cut(s) | 1170, 1366 |
| GlaI | GCGC | 2 cut(s) | 154, 179 |
| GluI | GCNGC | 5 cut(s) | 411, 843, 860, 1224, 1267 |
| GsaI | CCCAGC | 1 cut(s) | 410 |
| GsuI | CTGGAG | 1 cut(s) | 222 |
| HaeIII | GGCC | 3 cut(s) | 357, 776, 1120 |
| HapII | CCGG | 3 cut(s) | 200, 833, 1005 |
| HgaI | GACGC | 2 cut(s) | 220, 1347 |
| HhaI | GCGC | 2 cut(s) | 155, 180 |
| Hin1II | CATG | 6 cut(s) | 431, 568, 638, 680, 814, 1336 |
| Hin6I | GCGC | 2 cut(s) | 153, 178 |
| HinP1I | GCGC | 2 cut(s) | 153, 178 |
| HincII | GTYRAC | 1 cut(s) | 6 |
| HindII | GTYRAC | 1 cut(s) | 6 |
| HinfI | GANTC | 7 cut(s) | 11, 16, 23, 139, 829, 937, 1165 |
| HpaII | CCGG | 3 cut(s) | 200, 833, 1005 |
| HphI | GGTGA | 2 cut(s) | 334, 1150 |
| Hpy166II | GTNNAC | 3 cut(s) | 6, 306, 556 |
| Hpy188I | TCNGA | 8 cut(s) | 147, 171, 210, 324, 439, 485, 545, 1207 |
| Hpy188III | TCNNGA | 6 cut(s) | 20, 239, 635, 941, 976, 1333 |
| Hpy8I | GTNNAC | 3 cut(s) | 6, 306, 556 |
| Hpy99I | CGWCG | 1 cut(s) | 147 |
| HpyAV | CCTTC | 7 cut(s) | 412, 927, 1054, 1131, 1145, 1185, 1296 |
| HpyCH4III | ACNGT | 3 cut(s) | 610, 971, 1297 |
| HpyCH4IV | ACGT | 1 cut(s) | 1241 |
| HpyCH4V | TGCA | 8 cut(s) | 70, 128, 135, 187, 374, 676, 740, 1226 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 125, 184 |
| HpyF3I | CTNAG | 4 cut(s) | 103, 181, 542, 616 |
| HpySE526I | ACGT | 1 cut(s) | 1241 |
| Hsp92II | CATG | 6 cut(s) | 431, 568, 638, 680, 814, 1336 |
| HspAI | GCGC | 2 cut(s) | 153, 178 |
| KpnI | GGTACC | 1 cut(s) | 1263 |
| Kzo9I | GATC | 5 cut(s) | 189, 205, 228, 575, 663 |
| LmnI | GCTCC | 3 cut(s) | 50, 89, 1154 |
| Lsp1109I | GCAGC | 4 cut(s) | 829, 871, 1210, 1278 |
| LweI | GCATC | 1 cut(s) | 727 |
| MaeI | CTAG | 2 cut(s) | 1170, 1366 |
| MaeII | ACGT | 1 cut(s) | 1241 |
| MaeIII | GTNAC | 2 cut(s) | 232, 785 |
| MalI | GATC | 5 cut(s) | 191, 207, 230, 577, 665 |
| MbiI | CCGCTC | 1 cut(s) | 53 |
| MboI | GATC | 5 cut(s) | 189, 205, 228, 575, 663 |
| MboII | GAAGA | 6 cut(s) | 238, 498, 692, 968, 1003, 1348 |
| MfeI | CAATTG | 1 cut(s) | 549 |
| MhlI | GDGCHC | 1 cut(s) | 253 |
| MlsI | TGGCCA | 1 cut(s) | 776 |
| MluCI | AATT | 4 cut(s) | 346, 458, 549, 1010 |
| MluNI | TGGCCA | 1 cut(s) | 776 |
| MlyI | GAGTC | 4 cut(s) | 5, 10, 17, 823 |
| MmeI | TCCRAC | 4 cut(s) | 125, 1128, 1185, 1274 |
| Mox20I | TGGCCA | 1 cut(s) | 776 |
| MroXI | GAANNNNTTC | 2 cut(s) | 630, 726 |
| MscI | TGGCCA | 1 cut(s) | 776 |
| MslI | CAYNNNNRTG | 2 cut(s) | 880, 882 |
| Msp20I | TGGCCA | 1 cut(s) | 776 |
| MspA1I | CMGCKG | 2 cut(s) | 410, 862 |
| MspI | CCGG | 3 cut(s) | 200, 833, 1005 |
| MspR9I | CCNGG | 3 cut(s) | 200, 834, 1005 |
| MunI | CAATTG | 1 cut(s) | 549 |
| Mva1269I | GAATGC | 1 cut(s) | 909 |
| MvnI | CGCG | 1 cut(s) | 153 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 125, 184 |
| NciI | CCSGG | 3 cut(s) | 200, 834, 1005 |
| NdeII | GATC | 5 cut(s) | 189, 205, 228, 575, 663 |
| NlaIII | CATG | 6 cut(s) | 431, 568, 638, 680, 814, 1336 |
| NlaIV | GGNNCC | 3 cut(s) | 403, 866, 1261 |
| NmeAIII | GCCGAG | 2 cut(s) | 383, 989 |
| OliI | CACNNNNGTG | 1 cut(s) | 882 |
| PagI | TCATGA | 2 cut(s) | 634, 1332 |
| PceI | AGGCCT | 1 cut(s) | 1120 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 11 |
| PctI | GAATGC | 1 cut(s) | 909 |
| PdmI | GAANNNNTTC | 2 cut(s) | 630, 726 |
| PfeI | GAWTC | 3 cut(s) | 139, 937, 1165 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1256 |
| PkrI | GCNGC | 5 cut(s) | 412, 844, 861, 1225, 1268 |
| Ple19I | CGATCG | 1 cut(s) | 208 |
| PleI | GAGTC | 4 cut(s) | 5, 10, 17, 823 |
| PpsI | GAGTC | 4 cut(s) | 5, 10, 17, 823 |
| PsiI | TTATAA | 2 cut(s) | 272, 381 |
| PspFI | CCCAGC | 1 cut(s) | 406 |
| PspN4I | GGNNCC | 3 cut(s) | 403, 866, 1261 |
| PspPI | GGNCC | 1 cut(s) | 508 |
| PvuI | CGATCG | 1 cut(s) | 208 |
| RsaI | GTAC | 5 cut(s) | 60, 478, 563, 590, 1261 |
| RsaNI | GTAC | 5 cut(s) | 59, 477, 562, 589, 1260 |
| RseI | CAYNNNNRTG | 2 cut(s) | 880, 882 |
| SalI | GTCGAC | 1 cut(s) | 4 |
| SatI | GCNGC | 5 cut(s) | 411, 843, 860, 1224, 1267 |
| Sau3AI | GATC | 5 cut(s) | 189, 205, 228, 575, 663 |
| Sau96I | GGNCC | 1 cut(s) | 508 |
| ScaI | AGTACT | 1 cut(s) | 590 |
| SchI | GAGTC | 4 cut(s) | 5, 10, 17, 823 |
| ScrFI | CCNGG | 3 cut(s) | 200, 834, 1005 |
| SduI | GDGCHC | 1 cut(s) | 253 |
| SfaNI | GCATC | 1 cut(s) | 727 |
| SinI | GGWCC | 1 cut(s) | 508 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 880, 882 |
| Sse9I | AATT | 4 cut(s) | 346, 458, 549, 1010 |
| SseBI | AGGCCT | 1 cut(s) | 1120 |
| SsiI | CCGC | 4 cut(s) | 53, 410, 467, 862 |
| SspI | AATATT | 3 cut(s) | 112, 390, 727 |
| SspMI | CTAG | 2 cut(s) | 1170, 1366 |
| StuI | AGGCCT | 1 cut(s) | 1120 |
| StyD4I | CCNGG | 3 cut(s) | 198, 832, 1003 |
| TaaI | ACNGT | 3 cut(s) | 610, 971, 1297 |
| TaiI | ACGT | 1 cut(s) | 1244 |
| TaqI | TCGA | 3 cut(s) | 5, 14, 954 |
| TasI | AATT | 4 cut(s) | 346, 458, 549, 1010 |
| TatI | WGTACW | 2 cut(s) | 561, 588 |
| TauI | GCSGC | 1 cut(s) | 413 |
| TfiI | GAWTC | 3 cut(s) | 139, 937, 1165 |
| TscAI | CASTG | 3 cut(s) | 135, 420, 615 |
| TseI | GCWGC | 4 cut(s) | 842, 859, 1223, 1266 |
| TspDTI | ATGAA | 4 cut(s) | 468, 623, 693, 1349 |
| TspGWI | ACGGA | 3 cut(s) | 236, 500, 709 |
| TspRI | CASTG | 3 cut(s) | 135, 420, 615 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1256 |
| VpaK11BI | GGWCC | 1 cut(s) | 508 |
| XmiI | GTMKAC | 1 cut(s) | 5 |
| XmnI | GAANNNNTTC | 2 cut(s) | 630, 726 |
| XspI | CTAG | 2 cut(s) | 1170, 1366 |
| ZrmI | AGTACT | 1 cut(s) | 590 |