belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 348 |
| Acc65I | GGTACC | 1 cut(s) | 398 |
| AccB1I | GGYRCC | 1 cut(s) | 398 |
| AciI | CCGC | 2 cut(s) | 32, 434 |
| AclWI | GGATC | 1 cut(s) | 206 |
| AcoI | YGGCCR | 1 cut(s) | 738 |
| AcsI | RAATTY | 1 cut(s) | 706 |
| AfaI | GTAC | 3 cut(s) | 400, 445, 857 |
| AfeI | AGCGCT | 1 cut(s) | 516 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 947 |
| AgsI | TTSAA | 2 cut(s) | 167, 965 |
| AjiI | CACGTC | 1 cut(s) | 185 |
| AjnI | CCWGG | 2 cut(s) | 682, 796 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 594, 626, 921, 953 |
| AleI | CACNNNNGTG | 1 cut(s) | 846 |
| AluBI | AGCT | 6 cut(s) | 204, 595, 620, 745, 861, 968 |
| AluI | AGCT | 6 cut(s) | 204, 595, 620, 745, 861, 968 |
| Alw26I | GTCTC | 2 cut(s) | 485, 496 |
| AlwI | GGATC | 1 cut(s) | 206 |
| Aor51HI | AGCGCT | 1 cut(s) | 516 |
| AoxI | GGCC | 1 cut(s) | 738 |
| ApeKI | GCWGC | 3 cut(s) | 201, 655, 806 |
| ApoI | RAATTY | 1 cut(s) | 706 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 594, 710 |
| Asp718I | GGTACC | 1 cut(s) | 398 |
| AspLEI | GCGC | 1 cut(s) | 517 |
| AsuHPI | GGTGA | 1 cut(s) | 983 |
| AsuII | TTCGAA | 1 cut(s) | 588 |
| BalI | TGGCCA | 1 cut(s) | 740 |
| BanI | GGYRCC | 1 cut(s) | 398 |
| BbvI | GCAGC | 3 cut(s) | 213, 667, 793 |
| BccI | CCATC | 2 cut(s) | 272, 945 |
| BceAI | ACGGC | 4 cut(s) | 5, 23, 41, 53 |
| BciT130I | CCWGG | 2 cut(s) | 684, 798 |
| BclI | TGATCA | 1 cut(s) | 187 |
| BcoDI | GTCTC | 2 cut(s) | 485, 496 |
| BfaI | CTAG | 3 cut(s) | 392, 621, 977 |
| BfoI | RGCGCY | 1 cut(s) | 518 |
| BglII | AGATCT | 1 cut(s) | 576 |
| BisI | GCNGC | 3 cut(s) | 202, 656, 807 |
| BlsI | GCNGC | 3 cut(s) | 203, 657, 808 |
| Bme1390I | CCNGG | 2 cut(s) | 684, 798 |
| BmgBI | CACGTC | 1 cut(s) | 185 |
| BmiI | GGNNCC | 2 cut(s) | 400, 902 |
| BmrFI | CCNGG | 2 cut(s) | 684, 798 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 778, 810 |
| Bpu14I | TTCGAA | 1 cut(s) | 588 |
| BsaBI | GATNNNNATC | 1 cut(s) | 155 |
| BsaI | GGTCTC | 1 cut(s) | 496 |
| BsaJI | CCNNGG | 3 cut(s) | 484, 565, 941 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 96, 126 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 947 |
| Bse1I | ACTGG | 1 cut(s) | 938 |
| Bse8I | GATNNNNATC | 1 cut(s) | 155 |
| BseBI | CCWGG | 2 cut(s) | 684, 798 |
| BseDI | CCNNGG | 3 cut(s) | 484, 565, 941 |
| BseGI | GGATG | 2 cut(s) | 706, 956 |
| BseJI | GATNNNNATC | 1 cut(s) | 155 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 947 |
| BseNI | ACTGG | 1 cut(s) | 938 |
| BseXI | GCAGC | 3 cut(s) | 213, 667, 793 |
| BseYI | CCCAGC | 1 cut(s) | 823 |
| BsgI | GTGCAG | 2 cut(s) | 129, 792 |
| BshFI | GGCC | 1 cut(s) | 740 |
| BshNI | GGYRCC | 1 cut(s) | 398 |
| BslFI | GGGAC | 1 cut(s) | 628 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 947 |
| BsmAI | GTCTC | 2 cut(s) | 485, 496 |
| BsmBI | CGTCTC | 1 cut(s) | 485 |
| BsmFI | GGGAC | 1 cut(s) | 628 |
| BsmI | GAATGC | 2 cut(s) | 653, 873 |
| BsnI | GGCC | 1 cut(s) | 740 |
| Bso31I | GGTCTC | 1 cut(s) | 496 |
| Bsp119I | TTCGAA | 1 cut(s) | 588 |
| Bsp1407I | TGTACA | 1 cut(s) | 855 |
| Bsp143I | GATC | 3 cut(s) | 187, 211, 576 |
| Bsp19I | CCATGG | 2 cut(s) | 484, 565 |
| BspACI | CCGC | 2 cut(s) | 32, 434 |
| BspANI | GGCC | 1 cut(s) | 740 |
| BspHI | TCATGA | 1 cut(s) | 598 |
| BspLI | GGNNCC | 2 cut(s) | 400, 902 |
| BspPI | GGATC | 1 cut(s) | 206 |
| BspT104I | TTCGAA | 1 cut(s) | 588 |
| BspT107I | GGYRCC | 1 cut(s) | 398 |
| BspTNI | GGTCTC | 1 cut(s) | 496 |
| BsrGI | TGTACA | 1 cut(s) | 855 |
| BsrI | ACTGG | 1 cut(s) | 938 |
| BssECI | CCNNGG | 3 cut(s) | 484, 565, 941 |
| BssMI | GATC | 3 cut(s) | 187, 211, 576 |
| BssT1I | CCWWGG | 3 cut(s) | 484, 565, 941 |
| Bst2UI | CCWGG | 2 cut(s) | 684, 798 |
| Bst4CI | ACNGT | 2 cut(s) | 79, 163 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 116 |
| BstAUI | TGTACA | 1 cut(s) | 855 |
| BstBI | TTCGAA | 1 cut(s) | 588 |
| BstC8I | GCNNGC | 1 cut(s) | 653 |
| BstDEI | CTNAG | 1 cut(s) | 580 |
| BstDSI | CCRYGG | 2 cut(s) | 484, 565 |
| BstF5I | GGATG | 2 cut(s) | 706, 956 |
| BstH2I | RGCGCY | 1 cut(s) | 518 |
| BstHHI | GCGC | 1 cut(s) | 517 |
| BstKTI | GATC | 3 cut(s) | 190, 214, 579 |
| BstMAI | GTCTC | 2 cut(s) | 485, 496 |
| BstMBI | GATC | 3 cut(s) | 187, 211, 576 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 80, 116 |
| BstNI | CCWGG | 2 cut(s) | 684, 798 |
| BstSCI | CCNGG | 2 cut(s) | 682, 796 |
| BstV1I | GCAGC | 3 cut(s) | 213, 667, 793 |
| BstX2I | RGATCY | 1 cut(s) | 576 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 819 |
| BstYI | RGATCY | 1 cut(s) | 576 |
| BsuRI | GGCC | 1 cut(s) | 740 |
| BtgI | CCRYGG | 2 cut(s) | 484, 565 |
| BtrI | CACGTC | 1 cut(s) | 185 |
| BtsCI | GGATG | 2 cut(s) | 706, 956 |
| BtsI | GCAGTG | 1 cut(s) | 387 |
| BtsIMutI | CAGTG | 2 cut(s) | 75, 387 |
| Cac8I | GCNNGC | 1 cut(s) | 653 |
| CciI | TCATGA | 1 cut(s) | 598 |
| CfoI | GCGC | 1 cut(s) | 517 |
| Csp6I | GTAC | 3 cut(s) | 399, 444, 856 |
| CviAII | CATG | 5 cut(s) | 430, 464, 485, 566, 599 |
| CviQI | GTAC | 3 cut(s) | 399, 444, 856 |
| DdeI | CTNAG | 1 cut(s) | 580 |
| DpnI | GATC | 3 cut(s) | 189, 213, 578 |
| DpnII | GATC | 3 cut(s) | 187, 211, 576 |
| EaeI | YGGCCR | 1 cut(s) | 738 |
| EciI | GGCGGA | 1 cut(s) | 449 |
| Eco130I | CCWWGG | 3 cut(s) | 484, 565, 941 |
| Eco31I | GGTCTC | 1 cut(s) | 496 |
| Eco47III | AGCGCT | 1 cut(s) | 516 |
| EcoRII | CCWGG | 2 cut(s) | 682, 796 |
| EcoT14I | CCWWGG | 3 cut(s) | 484, 565, 941 |
| ErhI | CCWWGG | 3 cut(s) | 484, 565, 941 |
| Esp3I | CGTCTC | 1 cut(s) | 485 |
| FaeI | CATG | 5 cut(s) | 433, 467, 488, 569, 602 |
| FaqI | GGGAC | 1 cut(s) | 628 |
| FatI | CATG | 5 cut(s) | 429, 463, 484, 565, 598 |
| FbaI | TGATCA | 1 cut(s) | 187 |
| Fnu4HI | GCNGC | 3 cut(s) | 202, 656, 807 |
| FokI | GGATG | 2 cut(s) | 713, 963 |
| Fsp4HI | GCNGC | 3 cut(s) | 202, 656, 807 |
| FspBI | CTAG | 3 cut(s) | 392, 621, 977 |
| GlaI | GCGC | 1 cut(s) | 516 |
| GluI | GCNGC | 3 cut(s) | 202, 656, 807 |
| GsaI | CCCAGC | 1 cut(s) | 827 |
| HaeII | RGCGCY | 1 cut(s) | 518 |
| HaeIII | GGCC | 1 cut(s) | 740 |
| HhaI | GCGC | 1 cut(s) | 517 |
| Hin1II | CATG | 5 cut(s) | 433, 467, 488, 569, 602 |
| Hin6I | GCGC | 1 cut(s) | 515 |
| HinP1I | GCGC | 1 cut(s) | 515 |
| HindIII | AAGCTT | 1 cut(s) | 593 |
| HinfI | GANTC | 3 cut(s) | 178, 457, 585 |
| HphI | GGTGA | 1 cut(s) | 983 |
| Hpy166II | GTNNAC | 5 cut(s) | 273, 446, 667, 856, 974 |
| Hpy188I | TCNGA | 4 cut(s) | 147, 192, 216, 291 |
| Hpy188III | TCNNGA | 5 cut(s) | 6, 327, 548, 599, 880 |
| Hpy8I | GTNNAC | 5 cut(s) | 273, 446, 667, 856, 974 |
| Hpy99I | CGWCG | 1 cut(s) | 186 |
| HpyAV | CCTTC | 2 cut(s) | 379, 891 |
| HpyCH4III | ACNGT | 2 cut(s) | 79, 163 |
| HpyCH4IV | ACGT | 1 cut(s) | 184 |
| HpyCH4V | TGCA | 6 cut(s) | 110, 296, 608, 640, 750, 809 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 80, 116 |
| HpyF3I | CTNAG | 1 cut(s) | 580 |
| HpySE526I | ACGT | 1 cut(s) | 184 |
| Hsp92II | CATG | 5 cut(s) | 433, 467, 488, 569, 602 |
| HspAI | GCGC | 1 cut(s) | 515 |
| KpnI | GGTACC | 1 cut(s) | 402 |
| Ksp22I | TGATCA | 1 cut(s) | 187 |
| Kzo9I | GATC | 3 cut(s) | 187, 211, 576 |
| Lsp1109I | GCAGC | 3 cut(s) | 213, 667, 793 |
| MaeI | CTAG | 3 cut(s) | 392, 621, 977 |
| MaeII | ACGT | 1 cut(s) | 184 |
| MaeIII | GTNAC | 1 cut(s) | 95 |
| MalI | GATC | 3 cut(s) | 189, 213, 578 |
| MboI | GATC | 3 cut(s) | 187, 211, 576 |
| MboII | GAAGA | 4 cut(s) | 656, 703, 906, 967 |
| MfeI | CAATTG | 1 cut(s) | 414 |
| MflI | RGATCY | 1 cut(s) | 576 |
| MlsI | TGGCCA | 1 cut(s) | 740 |
| MluCI | AATT | 7 cut(s) | 10, 232, 414, 425, 469, 603, 706 |
| MluNI | TGGCCA | 1 cut(s) | 740 |
| MmeI | TCCRAC | 1 cut(s) | 239 |
| MnlI | CCTC | 6 cut(s) | 98, 168, 323, 412, 617, 780 |
| Mox20I | TGGCCA | 1 cut(s) | 740 |
| MroXI | GAANNNNTTC | 2 cut(s) | 594, 710 |
| MscI | TGGCCA | 1 cut(s) | 740 |
| MseI | TTAA | 1 cut(s) | 912 |
| MslI | CAYNNNNRTG | 1 cut(s) | 846 |
| Msp20I | TGGCCA | 1 cut(s) | 740 |
| MspR9I | CCNGG | 2 cut(s) | 684, 798 |
| MunI | CAATTG | 1 cut(s) | 414 |
| Mva1269I | GAATGC | 2 cut(s) | 653, 873 |
| MvaI | CCWGG | 2 cut(s) | 684, 798 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 80, 116 |
| NcoI | CCATGG | 2 cut(s) | 484, 565 |
| NdeII | GATC | 3 cut(s) | 187, 211, 576 |
| NlaIII | CATG | 5 cut(s) | 433, 467, 488, 569, 602 |
| NlaIV | GGNNCC | 2 cut(s) | 400, 902 |
| NspV | TTCGAA | 1 cut(s) | 588 |
| OliI | CACNNNNGTG | 1 cut(s) | 846 |
| PagI | TCATGA | 1 cut(s) | 598 |
| PctI | GAATGC | 2 cut(s) | 653, 873 |
| PdmI | GAANNNNTTC | 2 cut(s) | 594, 710 |
| PfeI | GAWTC | 3 cut(s) | 178, 457, 585 |
| PkrI | GCNGC | 3 cut(s) | 203, 657, 808 |
| PsiI | TTATAA | 1 cut(s) | 348 |
| Psp6I | CCWGG | 2 cut(s) | 682, 796 |
| PspFI | CCCAGC | 1 cut(s) | 823 |
| PspGI | CCWGG | 2 cut(s) | 682, 796 |
| PspN4I | GGNNCC | 2 cut(s) | 400, 902 |
| PsuI | RGATCY | 1 cut(s) | 576 |
| RsaI | GTAC | 3 cut(s) | 400, 445, 857 |
| RsaNI | GTAC | 3 cut(s) | 399, 444, 856 |
| RseI | CAYNNNNRTG | 1 cut(s) | 846 |
| SaqAI | TTAA | 1 cut(s) | 912 |
| SatI | GCNGC | 3 cut(s) | 202, 656, 807 |
| Sau3AI | GATC | 3 cut(s) | 187, 211, 576 |
| ScrFI | CCNGG | 2 cut(s) | 684, 798 |
| SfuI | TTCGAA | 1 cut(s) | 588 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 846 |
| Sse9I | AATT | 7 cut(s) | 10, 232, 414, 425, 469, 603, 706 |
| SsiI | CCGC | 2 cut(s) | 32, 434 |
| SspI | AATATT | 1 cut(s) | 691 |
| SspMI | CTAG | 3 cut(s) | 392, 621, 977 |
| StyD4I | CCNGG | 2 cut(s) | 682, 796 |
| StyI | CCWWGG | 3 cut(s) | 484, 565, 941 |
| TaaI | ACNGT | 2 cut(s) | 79, 163 |
| TaiI | ACGT | 1 cut(s) | 187 |
| TaqI | TCGA | 5 cut(s) | 5, 181, 404, 588, 879 |
| TasI | AATT | 7 cut(s) | 10, 232, 414, 425, 469, 603, 706 |
| TatI | WGTACW | 2 cut(s) | 443, 855 |
| TfiI | GAWTC | 3 cut(s) | 178, 457, 585 |
| Tru1I | TTAA | 1 cut(s) | 912 |
| Tru9I | TTAA | 1 cut(s) | 912 |
| TscAI | CASTG | 2 cut(s) | 82, 387 |
| TseI | GCWGC | 3 cut(s) | 201, 655, 806 |
| TspDTI | ATGAA | 4 cut(s) | 418, 449, 587, 615 |
| TspRI | CASTG | 2 cut(s) | 82, 387 |
| XapI | RAATTY | 1 cut(s) | 706 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 948 |
| XmnI | GAANNNNTTC | 2 cut(s) | 594, 710 |
| XspI | CTAG | 3 cut(s) | 392, 621, 977 |