Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0000333

Physical Location & Seq

Genomic Coordinates Reverse (-)

14751943 .. 14764285

Gene Structure 12343 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g21220.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3345 bp

ATGACTATGATGACTCCTCCACCTGTAGATCAAGAGGACGAAGAGATGCTGGTGCCGTCTACGGATGTGGTCGTCGAAGGTCCTCAGCCCATGGAAGTAGCTCAAGTGGAGCCGGCTAGTACAGTTGATAATCAGCCTGTGGAGGAGCCTCCAACCATGAAATTCACTTGGACAATTGAGAACTTTTCTAGATTGAACACCAAAAAGCACTACTCTGACATGTTCATCGTCGGTGGTTATAAATGGCGGATACTGATTTTCCCTAAAGGAAACAATGTGGACTACTTGTCAATGTATTTGGATGTGGCGGATTCGCAGGCGCTGCCTTATGGTTGGAGTAGATATGCGCAGTTCAGCTTGGCGATAGTGAATCAGATTCACAACAAGTACTCAATTAGAAAGGATACACAGCACCAATTCAATGCACGAGAAAGTGATTGGGGATTCACATCATTCATGCCTCTCGGTGATCTTTATGACCCCGGTAGGGGTTACCTGGTGAATGATACAGTCGTTGTTGAAGCCGAGGTTGTTGTCCGTAAAGTCCTTGATTACTGGTCGTATGACTCGAAAAAGGAAACCGGTTATGTTGGGCTCAAGAATCAGGGAGCAACTTGTTATATGAATTCACTTCTCCAGACTTTATACCATATACCTTACTTCAGAAAGGCTGTGTATCATATGCCAACAACTGAGAATGACATGCCTTCAGGAAGCATTCCTTTGGCTCTTCAAAGTTTATTCTATAAGCTTCAATACAACGAAAGCAGTGTTGCAACTAAAGAACTAACCAAGTCTTTTGGATGGGATACATATGATTCGTTTATGCAACATGATGTGCAGGAACTCAATAGAGTTCTATGTGAAAAGCTTGAGGACAAAATGAAGGGAACTGTGGTGGAGGGTACAATACAGCAGTTATTTGAAGGACATCACATGAATTACATTGAATGCATCAATGTGGACTACAAATCTACAAGAAAGGAGTCATTTTATGATCTCCAGCTTGATGTAAAAGGATGTCGTGATGTTTACGCTTCTTTTGATAAGTATGTGGAAGTTGAACGTCTTGAGGGTGACAATAAGTACCATGCTGAAGAACATGGTTTGCAGGATGCTAAGAAGGGTGTCCTGTTTATTGACTTCCCTCCAGTTCTTCAGCTTCAGCTAAAGCGATTTGAGTATGATTTTATGAGGGACACTATGGTAAAGATAAATGATCGTTATGAATTTCCTCTTCAACTTGACCTTGATAGGGAGAATGGCAAATATTTGTCACCGGAATCAGATAAGAGTGTGCGGAACCTCTACACTCTTCATAGTGTCCTGGTTCATAGTGGTGGCGTGCATGGAGGACATTACTATGCTTTCATCAGGCCAACCCTTACTGATCAGTGGTATAAGTTTGATGATGAACGTGTGACAAAAGAGGATGTGAAGAGGGCATTAGAAGAACAGTATGGTGGCGAGGAAGAGATGCCGCAGACTAATCCTGGTTTTAATAATACTCCTTTCAAGTTTACAAAGTACTCGAATGCGTACATGCTTGTGTATATTCGGGATAGTGACAAGGACAAAATAATATGTGATGTGGATGAGAAGGACATAGCTGAACATCTAAGGGTAAGGTTGAAGAAAGAACAAGAGGAGAAGGAGGACAAGAGGAGATATAAAGCACAGGCCCATCTTTATACTATTATCAAGGTTGCTCGAGACGAGGACCTGGCAGAACAAATCGGAAGGGACATATATTTTGACCTTGTGGACCATGACAAAGTTCGTAGTTTTCGAATCCAGAAACAGACACCCTTTAACCTTTTCAAGGAGGAAGTTGCGAAAGAGTTGGGAATACCAGTACAATTTCAGCGATTCTGGATTTGGGCAAAGCGGCAAAACCACACATATCGGCCCAACCGACCATTGACACCCCAGGAAGAATTACAAACAGTTGGACAATTGAGGGAGGCATCAAATAAAACACACAATGCAGAGCTAAAGTTGTTTCTGGAAGTAGAGCTTGGGCCGGAGCAAGTTCCTATCCCTCCACCGGACAAAACCAAGGATGATATTCTACTTTTCTTTAAGCTCTATGAACCTGAGAAACGAGAATTACGGTTTGTTGGTCGGCTTTTTGTGAAGAGTTCTACCAAGCCAATAGAAATAATAGCAAAAATTAATCAAATGGCTGGTTTTGCTCCTGATGAAGAAATAGAACTCTATGAGGAAATAAAGTTTGAGCCTTGTATCATGTGTGAACATCTTGACAAGAGGACCTCATTTCGATTAAGTCAGATTGAAGATGGTGACATTATATGCTTTCAAAAACCTACTCCACTAGAAAGTGAAGAATGTAAATATCCTGATGTACCTTCATTTTTGGAATATGTACACAACCGTCAGATTGTTCATTTTCGCTCTTTGGAGAAACCAAAAGAAGATGATTTTTCTTTAGAATTGTCTAAGCTACACACTTATGATGATGTGGTGGAGAAGGTGGCTTCCCAAATTGGTTTGGACGATCCTACGAAAATCAGACTCACTGCGCATAACTGCTATTCTCAGCAACCTAAACCCCAACCCATCAAATATCGAGGAGTAGAGCATTTAACAGATATGCTAGTCCACTATAATCAAAGTTCTGATATTTTGTATTATGAAGTCTTGGACATCCCCCTGCCAGAATTACAAGGCCTTAAAAATCTGAAAGTTGCATTTCATCATGCTACAAAAGATGAGGTGGTGATTCACAATATAAGATTGCCCAAGCAAAGCACCGTTGGGGATGTGATTAATGTACTAAAAACGAAGGTAGAGCTATCTCAACCAAATGCAGAACTTCGGCTTCTTGAGGTGTTCTATCACAAGATCTACAAGATCTTCCCACACAGTGAAAAAATTGAGAATATAAATGACCAGTACTGGACATTGCGTGCAGAGGAGGCAAAAGAAGAGAAAAACTTGGGTGCTAATGATCGCTTGATTCATGTTTACCACTTCACGAAAGAGACCGCACAGAACCAGATGCAGGTACAGAATTTCGGGGAACCTTTCTTCTTGGTTATTCATGAAGGTGAAACTTTAGCTGAAGTGAAAGCGCGCATACAAAAGAAATTGCAGGTCCCTGATGAGGAGTTTGCAAAGTGGAAGTTTGCATTCTTGTCATTGGGTCGTCCCGAGTACCTACAGGACACTGATGTAGTTTCTAGTCGTTTTCAGAGAAGAGATGTTTATGGTGCTTGGGAGCAGTACCTGGGGTTGGAGCATGCTGATACTGCTCCAAAGAGATCATATGCAGTAAATCAGAACCGTCACACATATGAGAAGCCAGTTAAGATATACAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004843 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009867 GO:0009987 GO:0010033 GO:0016787 GO:0019538 GO:0019783 GO:0023052 GO:0032870 GO:0036459 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070011 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0071704 GO:0101005 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04068 ko05168 ko05169 ko05203 map04068 map05168 map05169 map05203

Pfam Domains

Protein Families

MATH UCH USP7_C2 USP7_ICP0_bdg

Protein Analysis

1115

Amino Acids

130.63

Weight (kDa)

5.55

Isoelectric Point (pI)

44.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MATH_2	PF22486	54 - 177	1.6e-39	MATH domain
MATH	PF00917	59 - 179	2.4e-18	MATH domain
UCH	PF00443	197 - 518	9.8e-60	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	197 - 472	5e-15	Ubiquitin carboxyl-terminal hydrolase
USP7_ICP0_bdg	PF12436	622 - 873	3.2e-78	ICP0-binding domain of Ubiquitin-specific protease 7
USP7_C2	PF14533	883 - 1101	4.5e-56	Ubiquitin-specific protease C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11910 AT3G11910 AT3G11910 AT3G11910 AT5G06600 AT5G06600 AT5G06600
fragaria_vesca	FvH4_3g26930 FvH4_3g39132 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220
malus_domestica	MD02G1261100.v1.1 MD03G1161100.v1.1 MD07G1059900.v1.1 MD11G1177100.v1.1 MD12G1047900.v1.1 MD12G1048000.v1.1 MD12G1048400.v1.1 MD12G1048500.v1.1 MD12G1048700.v1.1 MD12G1048800.v1.1 MD12G1056200.v1.1 MD14G1046300.v1.1 MD14G1046500.v1.1 MD15G1166900.v1.1
prunus_persica	Prupe.2G066200_v2.0.a1 Prupe.2G066200_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1
pyrus_communis	pycom02g22320 pycom03g11340 pycom07g04580 pycom11g14290 pycom11g15110 pycom12g04110 pycom12g04140 pycom12g04160 pycom12g04210 pycom12g04220 pycom12g04230 pycom12g04290 pycom12g04320 pycom12g04330 pycom12g04350 pycom12g04360 pycom14g03880 pycom14g03900 pycom16g18750
rosa_chinensis	RchiOBHm_Chr1g0324111 RchiOBHm_Chr1g0324241 RchiOBHm_Chr3g0477691 RchiOBHm_Chr5g0049371 RchiOBHm_Chr5g0070531
rosa_laevigata	RLG00000008612 RLG00000023698 RLG00000034613 RLG00000036139
rosa_multiflora	Rmu_co8271779.1_g000001 Rmu_sc0006187.1_g000014
rosa_roxburghii	Rroxscaffold_1G00010670 Rroxscaffold_1G00030450 Rroxscaffold_1G00031950 Rroxscaffold_1G00031970 Rroxscaffold_1G00032320 Rroxscaffold_1G00055490 Rroxscaffold_2G00083610 Rroxscaffold_2G00084480 Rroxscaffold_2G00115610 Rroxscaffold_3G00261320 Rroxscaffold_4G00312220 Rroxscaffold_4G00325820 Rroxscaffold_5G00339170 Rroxscaffold_5G00364030 Rroxscaffold_6G00403750
rosa_rugosa	Rorug03G0162700 Rorug03G0162800 Rorug03G0162900 Rorug05G0247700 Rorug05G0406600 Rorug05G0406800
rosa_samantha	Rh1AG060300 Rh3AG213000 Rh3BG246600 Rh3CG240400 Rh3DG239700 Rh5AG324400 Rh5AG462100 Rh5BG335500 Rh5BG480200 Rh5BG480300 Rh5CG360200 Rh5CG504500 Rh5CG504600 Rh5DG347800 Rh5DG492200 Rh5DG492300
rosa_wichuraiana	Rw3G019270 Rw3G026910 Rw5G030650 Rw5G032610 Rw5G043040

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	240
Acc16I	TGCGCA	2 cut(s)	348, 2544
Acc36I	ACCTGC	2 cut(s)	3016, 3106
AccB1I	GGYRCC	1 cut(s)	52
AccI	GTMKAC	1 cut(s)	59
AccII	CGCG	1 cut(s)	3097
AciI	CCGC	6 cut(s)	247, 308, 1300, 1481, 1888, 3009
AclWI	GGATC	1 cut(s)	2513
AcsI	RAATTY	4 cut(s)	161, 625, 1229, 3034
AcuI	CTGAAG	6 cut(s)	646, 693, 1116, 1142, 1148, 3105
AdeI	CACNNNGTG	1 cut(s)	2888
AfiI	CCNNNNNNNGG	8 cut(s)	487, 488, 1255, 1822, 2047, 3025, 3127, 3256
AflIII	ACRYGT	2 cut(s)	219, 1417
AgeI	ACCGGT	1 cut(s)	581
AhdI	GACNNNNNGTC	1 cut(s)	286
AjnI	CCWGG	6 cut(s)	495, 1326, 1492, 1722, 1929, 3249
AjuI	GAANNNNNNNTTGG	2 cut(s)	2001, 2033
Alw26I	GTCTC	2 cut(s)	1707, 2999
AlwI	GGATC	1 cut(s)	2513
AlwNI	CAGNNNCTG	2 cut(s)	322, 3250
Ama87I	CYCGRG	2 cut(s)	1710, 3173
AoxI	GGCC	5 cut(s)	1376, 1680, 1907, 2021, 2689
ApeKI	GCWGC	1 cut(s)	322
ApoI	RAATTY	4 cut(s)	161, 625, 1229, 3034
ArsI	GACNNNNNNTTYG	2 cut(s)	1736, 1768
AseI	ATTAAT	2 cut(s)	2175, 2790
AsiGI	ACCGGT	1 cut(s)	581
AspLEI	GCGC	5 cut(s)	322, 349, 2545, 3097, 3099
AspS9I	GGNCC	8 cut(s)	80, 1681, 1720, 1765, 1908, 2021, 2271, 3118
AsuC2I	CCSGG	1 cut(s)	483
AsuHPI	GGTGA	7 cut(s)	479, 511, 1088, 1269, 2315, 2752, 3083
AsuII	TTCGAA	1 cut(s)	1789
AvaI	CYCGRG	2 cut(s)	1710, 3173
AvaII	GGWCC	5 cut(s)	80, 1720, 1765, 2271, 3118
BaeI	ACNNNNGTAYC	2 cut(s)	498, 531
BanI	GGYRCC	1 cut(s)	52
BanII	GRGCYC	1 cut(s)	597
BauI	CACGAG	1 cut(s)	426
BbvCI	CCTCAGC	1 cut(s)	84
BbvI	GCAGC	1 cut(s)	309
BccI	CCATC	4 cut(s)	798, 1692, 2294, 2588
BceAI	ACGGC	1 cut(s)	40
BciT130I	CCWGG	6 cut(s)	497, 1328, 1494, 1724, 1931, 3251
BciVI	GTATCC	3 cut(s)	243, 397, 802
BclI	TGATCA	1 cut(s)	1390
BcnI	CCSGG	1 cut(s)	483
BcoDI	GTCTC	2 cut(s)	1707, 2999
BfaI	CTAG	5 cut(s)	117, 189, 2336, 2618, 3204
BfmI	CTRYAG	2 cut(s)	24, 3182
BfoI	RGCGCY	1 cut(s)	323
BfuAI	ACCTGC	2 cut(s)	3016, 3106
BfuI	GTATCC	3 cut(s)	243, 397, 802
BglII	AGATCT	2 cut(s)	2865, 2874
BisI	GCNGC	3 cut(s)	323, 1481, 1889
BlsI	GCNGC	3 cut(s)	324, 1482, 1890
BmcAI	AGTACT	3 cut(s)	389, 1529, 2918
Bme1390I	CCNGG	7 cut(s)	483, 497, 1328, 1494, 1724, 1931, 3251
Bme18I	GGWCC	5 cut(s)	80, 1720, 1765, 2271, 3118
BmeRI	GACNNNNNGTC	1 cut(s)	286
BmeT110I	CYCGRG	2 cut(s)	1710, 3173
BmgT120I	GGNCC	8 cut(s)	80, 1681, 1720, 1765, 1908, 2021, 2271, 3118
BmiI	GGNNCC	6 cut(s)	54, 111, 147, 1304, 3045, 3120
BmrFI	CCNGG	7 cut(s)	483, 497, 1328, 1494, 1724, 1931, 3251
BmsI	GCATC	6 cut(s)	36, 963, 1105, 1467, 1976, 3012
BpmI	CTGGAG	3 cut(s)	620, 986, 1134
Bpu10I	CCTNAGC	1 cut(s)	84
Bpu14I	TTCGAA	1 cut(s)	1789
BpuEI	CTTGAG	5 cut(s)	87, 581, 893, 1091, 2867
BpuMI	CCSGG	1 cut(s)	483
BsaBI	GATNNNNATC	1 cut(s)	448
BsaI	GGTCTC	1 cut(s)	2999
BsaJI	CCNNGG	6 cut(s)	90, 481, 525, 1929, 2058, 3250
BsaWI	WCCGGW	3 cut(s)	581, 1279, 2047
Bsc4I	CCNNNNNNNGG	8 cut(s)	487, 488, 1255, 1822, 2047, 3025, 3127, 3256
Bse118I	RCCGGY	2 cut(s)	112, 581
Bse1I	ACTGG	6 cut(s)	560, 1151, 1853, 2914, 2924, 3326
Bse3DI	GCAATG	1 cut(s)	2924
Bse8I	GATNNNNATC	1 cut(s)	448
BseBI	CCWGG	6 cut(s)	497, 1328, 1494, 1724, 1931, 3251
BseDI	CCNNGG	6 cut(s)	90, 481, 525, 1929, 2058, 3250
BseJI	GATNNNNATC	1 cut(s)	448
BseLI	CCNNNNNNNGG	8 cut(s)	487, 488, 1255, 1822, 2047, 3025, 3127, 3256
BseMI	GCAATG	1 cut(s)	2924
BseMII	CTCAG	4 cut(s)	98, 684, 2088, 2573
BseNI	ACTGG	6 cut(s)	560, 1151, 1853, 2914, 2924, 3326
BsePI	GCGCGC	1 cut(s)	3095
BseRI	GAGGAG	7 cut(s)	6, 158, 1661, 1678, 2607, 2951, 3143
BseXI	GCAGC	1 cut(s)	309
BsgI	GTGCAG	2 cut(s)	860, 2952
Bsh1236I	CGCG	1 cut(s)	3097
BshFI	GGCC	5 cut(s)	1378, 1682, 1909, 2023, 2691
BshNI	GGYRCC	1 cut(s)	52
BshTI	ACCGGT	1 cut(s)	581
BsiHKCI	CYCGRG	2 cut(s)	1710, 3173
BsiSI	CCGG	6 cut(s)	113, 483, 582, 1280, 2024, 2048
BslFI	GGGAC	4 cut(s)	1211, 1757, 3104, 3156
BslI	CCNNNNNNNGG	8 cut(s)	487, 488, 1255, 1822, 2047, 3025, 3127, 3256
BsmAI	GTCTC	2 cut(s)	1707, 2999
BsmBI	CGTCTC	1 cut(s)	1707
BsmFI	GGGAC	4 cut(s)	1211, 1757, 3104, 3156
BsmI	GAATGC	4 cut(s)	717, 956, 1540, 3152
BsnI	GGCC	5 cut(s)	1378, 1682, 1909, 2023, 2691
Bso31I	GGTCTC	1 cut(s)	2999
BsoBI	CYCGRG	2 cut(s)	1710, 3173
Bsp119I	TTCGAA	1 cut(s)	1789
Bsp1286I	GDGCHC	1 cut(s)	597
Bsp1407I	TGTACA	1 cut(s)	2386
Bsp19I	CCATGG	1 cut(s)	90
BspACI	CCGC	6 cut(s)	247, 308, 1300, 1481, 1888, 3009
BspANI	GGCC	5 cut(s)	1378, 1682, 1909, 2023, 2691
BspCNI	CTCAG	4 cut(s)	97, 685, 2089, 2572
BspFNI	CGCG	1 cut(s)	3097
BspHI	TCATGA	1 cut(s)	3064
BspLI	GGNNCC	6 cut(s)	54, 111, 147, 1304, 3045, 3120
BspMI	ACCTGC	2 cut(s)	3016, 3106
BspPI	GGATC	1 cut(s)	2513
BspQI	GCTCTTC	1 cut(s)	735
BspT104I	TTCGAA	1 cut(s)	1789
BspT107I	GGYRCC	1 cut(s)	52
BspTNI	GGTCTC	1 cut(s)	2999
BsrDI	GCAATG	1 cut(s)	2924
BsrFI	RCCGGY	2 cut(s)	112, 581
BsrGI	TGTACA	1 cut(s)	2386
BsrI	ACTGG	6 cut(s)	560, 1151, 1853, 2914, 2924, 3326
BssAI	RCCGGY	2 cut(s)	112, 581
BssECI	CCNNGG	6 cut(s)	90, 481, 525, 1929, 2058, 3250
BssHII	GCGCGC	1 cut(s)	3095
BssSI	CACGAG	1 cut(s)	426
BssT1I	CCWWGG	2 cut(s)	90, 2058
Bst2BI	CACGAG	1 cut(s)	426
Bst2UI	CCWGG	6 cut(s)	497, 1328, 1494, 1724, 1931, 3251
Bst6I	CTCTTC	9 cut(s)	36, 735, 1242, 1320, 1433, 1467, 2132, 2943, 3214
BstAPI	GCANNNNNTGC	1 cut(s)	322
BstAUI	TGTACA	1 cut(s)	2386
BstBI	TTCGAA	1 cut(s)	1789
BstC8I	GCNNGC	6 cut(s)	114, 318, 1346, 2931, 3097, 3264
BstDEI	CTNAG	7 cut(s)	84, 693, 1119, 1619, 2097, 2460, 2559
BstDSI	CCRYGG	1 cut(s)	90
BstEII	GGTNACC	1 cut(s)	491
BstENI	CCTNNNNNAGG	1 cut(s)	1820
BstFNI	CGCG	1 cut(s)	3097
BstH2I	RGCGCY	1 cut(s)	323
BstHHI	GCGC	5 cut(s)	322, 349, 2545, 3097, 3099
BstMAI	GTCTC	2 cut(s)	1707, 2999
BstMWI	GCNNNNNNNGC	4 cut(s)	322, 2192, 2939, 3272
BstNI	CCWGG	6 cut(s)	497, 1328, 1494, 1724, 1931, 3251
BstNSI	RCATGY	4 cut(s)	223, 706, 1546, 3266
BstPI	GGTNACC	1 cut(s)	491
BstSCI	CCNGG	7 cut(s)	481, 495, 1326, 1492, 1722, 1929, 3249
BstSFI	CTRYAG	2 cut(s)	24, 3182
BstUI	CGCG	1 cut(s)	3097
BstV1I	GCAGC	1 cut(s)	309
BstX2I	RGATCY	2 cut(s)	2865, 2874
BstYI	RGATCY	2 cut(s)	2865, 2874
BsuI	GTATCC	3 cut(s)	243, 397, 802
BsuRI	GGCC	5 cut(s)	1378, 1682, 1909, 2023, 2691
BtgI	CCRYGG	1 cut(s)	90
BtsI	GCAGTG	2 cut(s)	775, 2538
BtsIMutI	CAGTG	5 cut(s)	775, 1400, 2538, 2893, 3189
BveI	ACCTGC	2 cut(s)	3016, 3106
Cac8I	GCNNGC	6 cut(s)	114, 318, 1346, 2931, 3097, 3264
CaiI	CAGNNNCTG	2 cut(s)	322, 3250
CciI	TCATGA	1 cut(s)	3064
CfoI	GCGC	5 cut(s)	322, 349, 2545, 3097, 3099
Cfr10I	RCCGGY	2 cut(s)	112, 581
Cfr13I	GGNCC	8 cut(s)	80, 1681, 1720, 1765, 1908, 2021, 2271, 3118
CsiI	ACCWGGT	1 cut(s)	495
CspAI	ACCGGT	1 cut(s)	581
DdeI	CTNAG	7 cut(s)	84, 693, 1119, 1619, 2097, 2460, 2559
DraIII	CACNNNGTG	1 cut(s)	2888
DriI	GACNNNNNGTC	1 cut(s)	286
Eam1104I	CTCTTC	9 cut(s)	36, 735, 1242, 1320, 1433, 1467, 2132, 2943, 3214
Eam1105I	GACNNNNNGTC	1 cut(s)	286
EarI	CTCTTC	9 cut(s)	36, 735, 1242, 1320, 1433, 1467, 2132, 2943, 3214
EciI	GGCGGA	2 cut(s)	262, 323
Eco130I	CCWWGG	2 cut(s)	90, 2058
Eco147I	AGGCCT	1 cut(s)	2691
Eco24I	GRGCYC	1 cut(s)	597
Eco31I	GGTCTC	1 cut(s)	2999
Eco47I	GGWCC	5 cut(s)	80, 1720, 1765, 2271, 3118
Eco57I	CTGAAG	6 cut(s)	646, 693, 1116, 1142, 1148, 3105
Eco88I	CYCGRG	2 cut(s)	1710, 3173
Eco91I	GGTNACC	1 cut(s)	491
EcoNI	CCTNNNNNAGG	1 cut(s)	1820
EcoO109I	RGGNCCY	4 cut(s)	80, 1720, 2271, 3118
EcoO65I	GGTNACC	1 cut(s)	491
EcoRI	GAATTC	1 cut(s)	625
EcoRII	CCWGG	6 cut(s)	495, 1326, 1492, 1722, 1929, 3249
EcoT14I	CCWWGG	2 cut(s)	90, 2058
EcoT22I	ATGCAT	1 cut(s)	956
EcoT38I	GRGCYC	1 cut(s)	597
ErhI	CCWWGG	2 cut(s)	90, 2058
Esp3I	CGTCTC	1 cut(s)	1707
FalI	AAGNNNNNCTT	4 cut(s)	706, 738, 2001, 2033
FaqI	GGGAC	4 cut(s)	1211, 1757, 3104, 3156
FauNDI	CATATG	4 cut(s)	681, 814, 3289, 3316
FbaI	TGATCA	1 cut(s)	1390
FblI	GTMKAC	1 cut(s)	59
Fnu4HI	GCNGC	3 cut(s)	323, 1481, 1889
FriOI	GRGCYC	1 cut(s)	597
Fsp4HI	GCNGC	3 cut(s)	323, 1481, 1889
FspBI	CTAG	5 cut(s)	117, 189, 2336, 2618, 3204
FspI	TGCGCA	2 cut(s)	348, 2544
GlaI	GCGC	5 cut(s)	321, 348, 2544, 3096, 3098
GluI	GCNGC	3 cut(s)	323, 1481, 1889
GsuI	CTGGAG	3 cut(s)	620, 986, 1134
HaeII	RGCGCY	1 cut(s)	323
HaeIII	GGCC	5 cut(s)	1378, 1682, 1909, 2023, 2691
HapII	CCGG	6 cut(s)	113, 483, 582, 1280, 2024, 2048
HhaI	GCGC	5 cut(s)	322, 349, 2545, 3097, 3099
Hin6I	GCGC	5 cut(s)	320, 347, 2543, 3095, 3097
HinP1I	GCGC	5 cut(s)	320, 347, 2543, 3095, 3097
HindIII	AAGCTT	2 cut(s)	749, 869
HpaII	CCGG	6 cut(s)	113, 483, 582, 1280, 2024, 2048
HphI	GGTGA	7 cut(s)	479, 511, 1088, 1269, 2315, 2752, 3083
Hpy99I	CGWCG	2 cut(s)	77, 233
HpyCH4IV	ACGT	2 cut(s)	1066, 1417
HpyF10VI	GCNNNNNNNGC	4 cut(s)	322, 2192, 2939, 3272
HpyF3I	CTNAG	7 cut(s)	84, 693, 1119, 1619, 2097, 2460, 2559
HpySE526I	ACGT	2 cut(s)	1066, 1417
HspAI	GCGC	5 cut(s)	320, 347, 2543, 3095, 3097
KroI	GCCGGC	1 cut(s)	112
KroNI	GCCGGC	1 cut(s)	114
Ksp22I	TGATCA	1 cut(s)	1390
LguI	GCTCTTC	1 cut(s)	735
LmnI	GCTCC	8 cut(s)	109, 145, 608, 2026, 2200, 3241, 3259, 3280
Lsp1109I	GCAGC	1 cut(s)	309
LweI	GCATC	6 cut(s)	36, 963, 1105, 1467, 1976, 3012
MabI	ACCWGGT	1 cut(s)	495
MaeI	CTAG	5 cut(s)	117, 189, 2336, 2618, 3204
MaeII	ACGT	2 cut(s)	1066, 1417
MaeIII	GTNAC	7 cut(s)	491, 1076, 1275, 1420, 1565, 2303, 3308
MfeI	CAATTG	2 cut(s)	174, 1955
MflI	RGATCY	2 cut(s)	2865, 2874
MhlI	GDGCHC	1 cut(s)	597
MlyI	GAGTC	4 cut(s)	7, 560, 995, 2529
MmeI	TCCRAC	4 cut(s)	176, 314, 1930, 3237
Mph1103I	ATGCAT	1 cut(s)	956
MroNI	GCCGGC	1 cut(s)	112
MseI	TTAA	9 cut(s)	1500, 1812, 2082, 2175, 2285, 2606, 2694, 2790, 3330
MslI	CAYNNNNRTG	7 cut(s)	959, 1338, 1362, 1547, 2472, 3069, 3315
MspI	CCGG	6 cut(s)	113, 483, 582, 1280, 2024, 2048
MspR9I	CCNGG	7 cut(s)	483, 497, 1328, 1494, 1724, 1931, 3251
MunI	CAATTG	2 cut(s)	174, 1955
Mva1269I	GAATGC	4 cut(s)	717, 956, 1540, 3152
MvaI	CCWGG	6 cut(s)	497, 1328, 1494, 1724, 1931, 3251
MvnI	CGCG	1 cut(s)	3097
MwoI	GCNNNNNNNGC	4 cut(s)	322, 2192, 2939, 3272
NaeI	GCCGGC	1 cut(s)	114
NciI	CCSGG	1 cut(s)	483
NcoI	CCATGG	1 cut(s)	90
NdeI	CATATG	4 cut(s)	681, 814, 3289, 3316
NgoMIV	GCCGGC	1 cut(s)	112
NlaIV	GGNNCC	6 cut(s)	54, 111, 147, 1304, 3045, 3120
NmeAIII	GCCGAG	1 cut(s)	550
NmuCI	GTSAC	6 cut(s)	1076, 1275, 1420, 1565, 2303, 3308
NsbI	TGCGCA	2 cut(s)	348, 2544
NsiI	ATGCAT	1 cut(s)	956
NspI	RCATGY	4 cut(s)	223, 706, 1546, 3266
NspV	TTCGAA	1 cut(s)	1789
PaeI	GCATGC	1 cut(s)	3266
PaeR7I	CTCGAG	1 cut(s)	1710
PagI	TCATGA	1 cut(s)	3064
PauI	GCGCGC	1 cut(s)	3095
PceI	AGGCCT	1 cut(s)	2691
PciI	ACATGT	1 cut(s)	219
PciSI	GCTCTTC	1 cut(s)	735
PcsI	WCGNNNNNNNCGW	3 cut(s)	566, 1786, 1912
PctI	GAATGC	4 cut(s)	717, 956, 1540, 3152
PdiI	GCCGGC	1 cut(s)	114
PinAI	ACCGGT	1 cut(s)	581
PkrI	GCNGC	3 cut(s)	324, 1482, 1890
PleI	GAGTC	4 cut(s)	7, 560, 994, 2529
PpsI	GAGTC	4 cut(s)	7, 560, 994, 2529
PpuMI	RGGWCCY	4 cut(s)	80, 1720, 2271, 3118
PscI	ACATGT	1 cut(s)	219
PshBI	ATTAAT	2 cut(s)	2175, 2790
PsiI	TTATAA	1 cut(s)	240
Psp5II	RGGWCCY	4 cut(s)	80, 1720, 2271, 3118
Psp6I	CCWGG	6 cut(s)	495, 1326, 1492, 1722, 1929, 3249
PspEI	GGTNACC	1 cut(s)	491
PspGI	CCWGG	6 cut(s)	495, 1326, 1492, 1722, 1929, 3249
PspN4I	GGNNCC	6 cut(s)	54, 111, 147, 1304, 3045, 3120
PspPI	GGNCC	8 cut(s)	80, 1681, 1720, 1765, 1908, 2021, 2271, 3118
PspPPI	RGGWCCY	4 cut(s)	80, 1720, 2271, 3118
PstNI	CAGNNNCTG	2 cut(s)	322, 3250
PsuI	RGATCY	2 cut(s)	2865, 2874
PteI	GCGCGC	1 cut(s)	3095
RseI	CAYNNNNRTG	7 cut(s)	959, 1338, 1362, 1547, 2472, 3069, 3315
SapI	GCTCTTC	1 cut(s)	735
SaqAI	TTAA	9 cut(s)	1500, 1812, 2082, 2175, 2285, 2606, 2694, 2790, 3330
SatI	GCNGC	3 cut(s)	323, 1481, 1889
Sau96I	GGNCC	8 cut(s)	80, 1681, 1720, 1765, 1908, 2021, 2271, 3118
ScaI	AGTACT	3 cut(s)	389, 1529, 2918
SchI	GAGTC	4 cut(s)	7, 560, 995, 2529
ScrFI	CCNGG	7 cut(s)	483, 497, 1328, 1494, 1724, 1931, 3251
SduI	GDGCHC	1 cut(s)	597
SexAI	ACCWGGT	1 cut(s)	495
SfaNI	GCATC	6 cut(s)	36, 963, 1105, 1467, 1976, 3012
SfcI	CTRYAG	2 cut(s)	24, 3182
Sfr274I	CTCGAG	1 cut(s)	1710
SfuI	TTCGAA	1 cut(s)	1789
SinI	GGWCC	5 cut(s)	80, 1720, 1765, 2271, 3118
SlaI	CTCGAG	1 cut(s)	1710
SmiMI	CAYNNNNRTG	7 cut(s)	959, 1338, 1362, 1547, 2472, 3069, 3315
SmlI	CTYRAG	6 cut(s)	102, 596, 872, 1070, 1710, 2846
SmoI	CTYRAG	6 cut(s)	102, 596, 872, 1070, 1710, 2846
SphI	GCATGC	1 cut(s)	3266
SseBI	AGGCCT	1 cut(s)	2691
SsiI	CCGC	6 cut(s)	247, 308, 1300, 1481, 1888, 3009
SspI	AATATT	1 cut(s)	1271
SspMI	CTAG	5 cut(s)	117, 189, 2336, 2618, 3204
StuI	AGGCCT	1 cut(s)	2691
StyD4I	CCNGG	7 cut(s)	481, 495, 1326, 1492, 1722, 1929, 3249
StyI	CCWWGG	2 cut(s)	90, 2058
TaiI	ACGT	2 cut(s)	1069, 1420
TaqI	TCGA	7 cut(s)	75, 569, 1532, 1711, 1789, 2281, 2590
TatI	WGTACW	7 cut(s)	119, 387, 1527, 1855, 2386, 2794, 2916
TauI	GCSGC	2 cut(s)	1483, 1891
Tru1I	TTAA	9 cut(s)	1500, 1812, 2082, 2175, 2285, 2606, 2694, 2790, 3330
Tru9I	TTAA	9 cut(s)	1500, 1812, 2082, 2175, 2285, 2606, 2694, 2790, 3330
TscAI	CASTG	5 cut(s)	775, 1400, 2545, 2893, 3196
TseFI	GTSAC	6 cut(s)	1076, 1275, 1420, 1565, 2303, 3308
TseI	GCWGC	1 cut(s)	322
Tsp45I	GTSAC	6 cut(s)	1076, 1275, 1420, 1565, 2303, 3308
TspGWI	ACGGA	2 cut(s)	77, 527
TspRI	CASTG	5 cut(s)	775, 1400, 2545, 2893, 3196
VpaK11BI	GGWCC	5 cut(s)	80, 1720, 1765, 2271, 3118
VspI	ATTAAT	2 cut(s)	2175, 2790
XagI	CCTNNNNNAGG	1 cut(s)	1820
XapI	RAATTY	4 cut(s)	161, 625, 1229, 3034
XbaI	TCTAGA	1 cut(s)	188
XceI	RCATGY	4 cut(s)	223, 706, 1546, 3266
XhoI	CTCGAG	1 cut(s)	1710
XmiI	GTMKAC	1 cut(s)	59
XspI	CTAG	5 cut(s)	117, 189, 2336, 2618, 3204
ZrmI	AGTACT	3 cut(s)	389, 1529, 2918
Zsp2I	ATGCAT	1 cut(s)	956

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.