Ubiquitin carboxyl-terminal hydrolase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 3 cut(s) | 94, 330, 622 |
| AclWI | GGATC | 1 cut(s) | 561 |
| AcoI | YGGCCR | 2 cut(s) | 36, 448 |
| AcsI | RAATTY | 1 cut(s) | 13 |
| AcuI | CTGAAG | 2 cut(s) | 84, 555 |
| AcyI | GRCGYC | 1 cut(s) | 437 |
| AfaI | GTAC | 3 cut(s) | 9, 158, 227 |
| AfeI | AGCGCT | 1 cut(s) | 265 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 106, 235 |
| AgsI | TTSAA | 2 cut(s) | 476, 562 |
| AjnI | CCWGG | 2 cut(s) | 381, 492 |
| AluBI | AGCT | 3 cut(s) | 62, 254, 311 |
| AluI | AGCT | 3 cut(s) | 62, 254, 311 |
| Alw21I | GWGCWC | 1 cut(s) | 243 |
| Alw26I | GTCTC | 1 cut(s) | 449 |
| AlwI | GGATC | 1 cut(s) | 561 |
| Aor51HI | AGCGCT | 1 cut(s) | 265 |
| AoxI | GGCC | 3 cut(s) | 36, 448, 499 |
| ApeKI | GCWGC | 5 cut(s) | 295, 410, 545, 602, 636 |
| ApoI | RAATTY | 1 cut(s) | 13 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 538 |
| AspLEI | GCGC | 1 cut(s) | 266 |
| AspS9I | GGNCC | 2 cut(s) | 97, 500 |
| AsuHPI | GGTGA | 1 cut(s) | 62 |
| AvaII | GGWCC | 1 cut(s) | 97 |
| BalI | TGGCCA | 1 cut(s) | 38 |
| BanII | GRGCYC | 1 cut(s) | 325 |
| BauI | CACGAG | 2 cut(s) | 324, 452 |
| BbsI | GAAGAC | 2 cut(s) | 411, 541 |
| Bbv12I | GWGCWC | 1 cut(s) | 243 |
| BbvI | GCAGC | 5 cut(s) | 307, 397, 532, 589, 648 |
| BccI | CCATC | 3 cut(s) | 47, 491, 592 |
| BceAI | ACGGC | 1 cut(s) | 171 |
| BciT130I | CCWGG | 2 cut(s) | 383, 494 |
| BciVI | GTATCC | 1 cut(s) | 72 |
| BcoDI | GTCTC | 1 cut(s) | 449 |
| BfaI | CTAG | 1 cut(s) | 641 |
| BfmI | CTRYAG | 1 cut(s) | 546 |
| BfoI | RGCGCY | 1 cut(s) | 267 |
| BfuI | GTATCC | 1 cut(s) | 72 |
| BisI | GCNGC | 7 cut(s) | 296, 330, 411, 546, 603, 622, 637 |
| BlsI | GCNGC | 7 cut(s) | 297, 331, 412, 547, 604, 623, 638 |
| BmcAI | AGTACT | 1 cut(s) | 158 |
| Bme1390I | CCNGG | 2 cut(s) | 383, 494 |
| Bme18I | GGWCC | 1 cut(s) | 97 |
| BmgT120I | GGNCC | 2 cut(s) | 97, 500 |
| BmiI | GGNNCC | 3 cut(s) | 24, 98, 99 |
| BmrFI | CCNGG | 2 cut(s) | 383, 494 |
| BpiI | GAAGAC | 2 cut(s) | 411, 541 |
| BpmI | CTGGAG | 1 cut(s) | 495 |
| BsaHI | GRCGYC | 1 cut(s) | 437 |
| BsaJI | CCNNGG | 3 cut(s) | 229, 286, 432 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 106, 235 |
| BseBI | CCWGG | 2 cut(s) | 383, 494 |
| BseDI | CCNNGG | 3 cut(s) | 229, 286, 432 |
| BseGI | GGATG | 2 cut(s) | 148, 502 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 106, 235 |
| BseMII | CTCAG | 2 cut(s) | 144, 521 |
| BseXI | GCAGC | 5 cut(s) | 307, 397, 532, 589, 648 |
| BsgI | GTGCAG | 1 cut(s) | 635 |
| BshFI | GGCC | 3 cut(s) | 38, 450, 501 |
| BsiHKAI | GWGCWC | 1 cut(s) | 243 |
| BslFI | GGGAC | 1 cut(s) | 83 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 106, 235 |
| BsmAI | GTCTC | 1 cut(s) | 449 |
| BsmFI | GGGAC | 1 cut(s) | 83 |
| BsnI | GGCC | 3 cut(s) | 38, 450, 501 |
| Bsp1286I | GDGCHC | 2 cut(s) | 243, 325 |
| Bsp143I | GATC | 2 cut(s) | 520, 553 |
| Bsp19I | CCATGG | 1 cut(s) | 432 |
| BspACI | CCGC | 3 cut(s) | 94, 330, 622 |
| BspANI | GGCC | 3 cut(s) | 38, 450, 501 |
| BspCNI | CTCAG | 2 cut(s) | 145, 522 |
| BspLI | GGNNCC | 3 cut(s) | 24, 98, 99 |
| BspMAI | CTGCAG | 1 cut(s) | 550 |
| BspPI | GGATC | 1 cut(s) | 561 |
| BssECI | CCNNGG | 3 cut(s) | 229, 286, 432 |
| BssMI | GATC | 2 cut(s) | 520, 553 |
| BssNI | GRCGYC | 1 cut(s) | 437 |
| BssSI | CACGAG | 2 cut(s) | 324, 452 |
| BssT1I | CCWWGG | 3 cut(s) | 229, 286, 432 |
| Bst2BI | CACGAG | 2 cut(s) | 324, 452 |
| Bst2UI | CCWGG | 2 cut(s) | 383, 494 |
| Bst4CI | ACNGT | 1 cut(s) | 616 |
| Bst6I | CTCTTC | 1 cut(s) | 193 |
| BstACI | GRCGYC | 1 cut(s) | 437 |
| BstDEI | CTNAG | 6 cut(s) | 117, 153, 258, 315, 424, 530 |
| BstDSI | CCRYGG | 1 cut(s) | 432 |
| BstF5I | GGATG | 2 cut(s) | 148, 502 |
| BstH2I | RGCGCY | 1 cut(s) | 267 |
| BstHHI | GCGC | 1 cut(s) | 266 |
| BstKTI | GATC | 2 cut(s) | 523, 556 |
| BstMAI | GTCTC | 1 cut(s) | 449 |
| BstMBI | GATC | 2 cut(s) | 520, 553 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 329, 338, 512, 602 |
| BstNI | CCWGG | 2 cut(s) | 383, 494 |
| BstSCI | CCNGG | 2 cut(s) | 381, 492 |
| BstSFI | CTRYAG | 1 cut(s) | 546 |
| BstV1I | GCAGC | 5 cut(s) | 307, 397, 532, 589, 648 |
| BstV2I | GAAGAC | 2 cut(s) | 411, 541 |
| BstX2I | RGATCY | 1 cut(s) | 553 |
| BstYI | RGATCY | 1 cut(s) | 553 |
| BsuI | GTATCC | 1 cut(s) | 72 |
| BsuRI | GGCC | 3 cut(s) | 38, 450, 501 |
| BtgI | CCRYGG | 1 cut(s) | 432 |
| BtsCI | GGATG | 2 cut(s) | 148, 502 |
| BtsIMutI | CAGTG | 1 cut(s) | 612 |
| CfoI | GCGC | 1 cut(s) | 266 |
| Cfr13I | GGNCC | 2 cut(s) | 97, 500 |
| CseI | GACGC | 2 cut(s) | 416, 426 |
| Csp6I | GTAC | 3 cut(s) | 8, 157, 226 |
| CviAII | CATG | 3 cut(s) | 216, 269, 433 |
| CviQI | GTAC | 3 cut(s) | 8, 157, 226 |
| DdeI | CTNAG | 6 cut(s) | 117, 153, 258, 315, 424, 530 |
| DpnI | GATC | 2 cut(s) | 522, 555 |
| DpnII | GATC | 2 cut(s) | 520, 553 |
| EaeI | YGGCCR | 2 cut(s) | 36, 448 |
| Eam1104I | CTCTTC | 1 cut(s) | 193 |
| EarI | CTCTTC | 1 cut(s) | 193 |
| Eco130I | CCWWGG | 3 cut(s) | 229, 286, 432 |
| Eco24I | GRGCYC | 1 cut(s) | 325 |
| Eco47I | GGWCC | 1 cut(s) | 97 |
| Eco47III | AGCGCT | 1 cut(s) | 265 |
| Eco57I | CTGAAG | 2 cut(s) | 84, 555 |
| EcoO109I | RGGNCCY | 1 cut(s) | 97 |
| EcoRII | CCWGG | 2 cut(s) | 381, 492 |
| EcoT14I | CCWWGG | 3 cut(s) | 229, 286, 432 |
| EcoT38I | GRGCYC | 1 cut(s) | 325 |
| ErhI | CCWWGG | 3 cut(s) | 229, 286, 432 |
| FaeI | CATG | 3 cut(s) | 219, 272, 436 |
| FaiI | YATR | 6 cut(s) | 210, 217, 270, 434, 627, 667 |
| FaqI | GGGAC | 1 cut(s) | 83 |
| FatI | CATG | 3 cut(s) | 215, 268, 432 |
| FauI | CCCGC | 1 cut(s) | 87 |
| Fnu4HI | GCNGC | 7 cut(s) | 296, 330, 411, 546, 603, 622, 637 |
| FokI | GGATG | 2 cut(s) | 135, 509 |
| FriOI | GRGCYC | 1 cut(s) | 325 |
| Fsp4HI | GCNGC | 7 cut(s) | 296, 330, 411, 546, 603, 622, 637 |
| FspBI | CTAG | 1 cut(s) | 641 |
| GlaI | GCGC | 1 cut(s) | 265 |
| GluI | GCNGC | 7 cut(s) | 296, 330, 411, 546, 603, 622, 637 |
| GsuI | CTGGAG | 1 cut(s) | 495 |
| HaeII | RGCGCY | 1 cut(s) | 267 |
| HaeIII | GGCC | 3 cut(s) | 38, 450, 501 |
| HgaI | GACGC | 2 cut(s) | 416, 426 |
| HhaI | GCGC | 1 cut(s) | 266 |
| Hin1I | GRCGYC | 1 cut(s) | 437 |
| Hin1II | CATG | 3 cut(s) | 219, 272, 436 |
| Hin6I | GCGC | 1 cut(s) | 264 |
| HinP1I | GCGC | 1 cut(s) | 264 |
| HinfI | GANTC | 2 cut(s) | 167, 428 |
| HphI | GGTGA | 1 cut(s) | 62 |
| Hpy166II | GTNNAC | 1 cut(s) | 567 |
| Hpy188I | TCNGA | 5 cut(s) | 172, 195, 247, 304, 531 |
| Hpy188III | TCNNGA | 1 cut(s) | 152 |
| Hpy8I | GTNNAC | 1 cut(s) | 567 |
| HpyAV | CCTTC | 1 cut(s) | 41 |
| HpyCH4III | ACNGT | 1 cut(s) | 616 |
| HpyCH4V | TGCA | 9 cut(s) | 4, 163, 215, 272, 295, 548, 605, 629, 652 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 329, 338, 512, 602 |
| HpyF3I | CTNAG | 6 cut(s) | 117, 153, 258, 315, 424, 530 |
| Hsp92I | GRCGYC | 1 cut(s) | 437 |
| Hsp92II | CATG | 3 cut(s) | 219, 272, 436 |
| HspAI | GCGC | 1 cut(s) | 264 |
| KflI | GGGWCCC | 1 cut(s) | 97 |
| Kzo9I | GATC | 2 cut(s) | 520, 553 |
| LmnI | GCTCC | 5 cut(s) | 220, 238, 259, 316, 633 |
| Lsp1109I | GCAGC | 5 cut(s) | 307, 397, 532, 589, 648 |
| MaeI | CTAG | 1 cut(s) | 641 |
| MaeIII | GTNAC | 2 cut(s) | 138, 418 |
| MalI | GATC | 2 cut(s) | 522, 555 |
| MboI | GATC | 2 cut(s) | 520, 553 |
| MboII | GAAGA | 4 cut(s) | 22, 210, 416, 546 |
| MflI | RGATCY | 1 cut(s) | 553 |
| MhlI | GDGCHC | 2 cut(s) | 243, 325 |
| MlsI | TGGCCA | 1 cut(s) | 38 |
| MluCI | AATT | 3 cut(s) | 13, 89, 471 |
| MluNI | TGGCCA | 1 cut(s) | 38 |
| MlyI | GAGTC | 1 cut(s) | 437 |
| MmeI | TCCRAC | 2 cut(s) | 216, 392 |
| MnlI | CCTC | 4 cut(s) | 312, 382, 544, 586 |
| Mox20I | TGGCCA | 1 cut(s) | 38 |
| MroXI | GAANNNNTTC | 1 cut(s) | 538 |
| MscI | TGGCCA | 1 cut(s) | 38 |
| MseI | TTAA | 1 cut(s) | 69 |
| Msp20I | TGGCCA | 1 cut(s) | 38 |
| MspR9I | CCNGG | 2 cut(s) | 383, 494 |
| MvaI | CCWGG | 2 cut(s) | 383, 494 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 329, 338, 512, 602 |
| NcoI | CCATGG | 1 cut(s) | 432 |
| NdeII | GATC | 2 cut(s) | 520, 553 |
| NlaIII | CATG | 3 cut(s) | 219, 272, 436 |
| NlaIV | GGNNCC | 3 cut(s) | 24, 98, 99 |
| NmuCI | GTSAC | 1 cut(s) | 138 |
| PdmI | GAANNNNTTC | 1 cut(s) | 538 |
| PfeI | GAWTC | 1 cut(s) | 167 |
| PkrI | GCNGC | 7 cut(s) | 297, 331, 412, 547, 604, 623, 638 |
| PleI | GAGTC | 1 cut(s) | 436 |
| PpsI | GAGTC | 1 cut(s) | 436 |
| PpuMI | RGGWCCY | 1 cut(s) | 97 |
| Psp5II | RGGWCCY | 1 cut(s) | 97 |
| Psp6I | CCWGG | 2 cut(s) | 381, 492 |
| PspGI | CCWGG | 2 cut(s) | 381, 492 |
| PspN4I | GGNNCC | 3 cut(s) | 24, 98, 99 |
| PspPI | GGNCC | 2 cut(s) | 97, 500 |
| PspPPI | RGGWCCY | 1 cut(s) | 97 |
| PstI | CTGCAG | 1 cut(s) | 550 |
| PsuI | RGATCY | 1 cut(s) | 553 |
| RsaI | GTAC | 3 cut(s) | 9, 158, 227 |
| RsaNI | GTAC | 3 cut(s) | 8, 157, 226 |
| SaqAI | TTAA | 1 cut(s) | 69 |
| SatI | GCNGC | 7 cut(s) | 296, 330, 411, 546, 603, 622, 637 |
| Sau3AI | GATC | 2 cut(s) | 520, 553 |
| Sau96I | GGNCC | 2 cut(s) | 97, 500 |
| ScaI | AGTACT | 1 cut(s) | 158 |
| SchI | GAGTC | 1 cut(s) | 437 |
| ScrFI | CCNGG | 2 cut(s) | 383, 494 |
| SduI | GDGCHC | 2 cut(s) | 243, 325 |
| SfcI | CTRYAG | 1 cut(s) | 546 |
| SinI | GGWCC | 1 cut(s) | 97 |
| Sse9I | AATT | 3 cut(s) | 13, 89, 471 |
| SsiI | CCGC | 3 cut(s) | 94, 330, 622 |
| SspMI | CTAG | 1 cut(s) | 641 |
| StyD4I | CCNGG | 2 cut(s) | 381, 492 |
| StyI | CCWWGG | 3 cut(s) | 229, 286, 432 |
| TaaI | ACNGT | 1 cut(s) | 616 |
| TaqI | TCGA | 1 cut(s) | 523 |
| TasI | AATT | 3 cut(s) | 13, 89, 471 |
| TatI | WGTACW | 2 cut(s) | 7, 156 |
| TauI | GCSGC | 2 cut(s) | 332, 624 |
| TfiI | GAWTC | 1 cut(s) | 167 |
| Tru1I | TTAA | 1 cut(s) | 69 |
| Tru9I | TTAA | 1 cut(s) | 69 |
| TscAI | CASTG | 1 cut(s) | 619 |
| TseFI | GTSAC | 1 cut(s) | 138 |
| TseI | GCWGC | 5 cut(s) | 295, 410, 545, 602, 636 |
| Tsp45I | GTSAC | 1 cut(s) | 138 |
| TspDTI | ATGAA | 1 cut(s) | 60 |
| TspGWI | ACGGA | 1 cut(s) | 91 |
| TspRI | CASTG | 1 cut(s) | 619 |
| VpaK11BI | GGWCC | 1 cut(s) | 97 |
| XapI | RAATTY | 1 cut(s) | 13 |
| XmnI | GAANNNNTTC | 1 cut(s) | 538 |
| XspI | CTAG | 1 cut(s) | 641 |
| ZrmI | AGTACT | 1 cut(s) | 158 |