MD03G1161100.v1.1

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000333
Physical Location & Seq
Reverse (-)
19398699 .. 19418020
19322 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1161100.v1.1.491

Sequence Viewer

Length: 3354 bp
ATGACAGTCATGACGCCTGCCCCTATTGATCAACCAGAGGACGAGGAGATGCTTGTCCCACATTCGGATTTGCCCGAAAACAATCACCAGCCCATGGAAGTTGTTGCACAACCTGAAACTGCTAATAATGTGGAGAACCAGCCAGTGGAGGATCCTCCATCATCAAGATTCACATGGAGAATTGACAACTTTACTAGGATGAATACAAAGAAGCATTATTCAGATGTATTTGTAGTTGGCAGCTACAAATGGCGCGTGCTTATTTTTCCAAAGGGAAATAATGTGGATCATTTGTCCATGTATTTGGATGTTGCGGATTCTTCAAGTTTGCCATATGGGTGGAGCAGATATGCACAATTTAGCTTGGGGGTTATCAATCAAATTCATAACAAGTATTCAGTACGAAAAGACACACAACATCAGTTCAATGCGCGGGAAAGTGATTGGGGTTTTACATCCTTTATGCCTCTCAGTGAACTTTACGATCCCAGTAGAGGGTATCTTGTGAATGATACGATTATAGTTGAAGCCGAGGTTCTCGTTCGTAGGGTTGTAGATTACTGGACATATGACTCAAAAAAGGAGACAGGTTATGTGGGGCTTAAAAACCAAGGAGCAACGTGCTACATGAACTCCCTCCTCCAGACTTTGTATCATATTCCTTATTTCAGAAAGGCTGTTTACCATATGCCCACAACTGAGAATGATTTGCCGTCACAAAGCATACCATTGGCTCTTCAGAGTCTATTTTACAAGCTCCAGTACAGTGACAACAGTGTCGCAACCAAAGAGCTGACAAAGTCTTTTGGATGGGACACATATGATTCATTTATGCAGCATGATGTACAAGAACTCAATAGAGTTTTGTGTGAAAAACTTGAGGATAAAATGAAGGGAACTGTTGTAGAGGGAACTATACAGAAGTTATTTGAAGGACACCATATGAACTACATTGAATGTATCAACGTGGACTACAAATCTACAAGAAAGGAATCATTTTATGACCTTCAGCTTGACGTTAAAGGCTGTCGTGATGTTTATGCTTCTTTTGATAAGTATGTTGAAGTGGAACGTCTTGAGGGTGATAACAAGTATCATGCTGAACATTATGGATTACAGGATGCCAAGAAGGGTGTTCTGTTCATTGACTTCCCCCCTGTGCTTCAACTTCAACTGAAACGATTTGAGTACGACTTCATGCGGGACACAATGGTGAAGATTAATGATCGTTATGAGTTCCCGTTGCAACTTGACCTTGATCGAGAGAATGGAAAATATTTATCACCTGAAGCTGATAGGAGCGTTCGCAACCTTTACACACTTCACAGTGTTTTGGTGCATAGTGGTGGGGTGCACGGTGGACACTATTATGCCTTTATCAGGCCAACACTTACTGATCTGTGGTATAAATTTGACGATGAGAGGGTAACTAAAGAAGATATAAAGAGGGCACTAGAGGAGCAGTATGGTGGTGAGGAAGAGTTACCGCAGACAAACCCTGGATTTAATAATACTCCTTTCAAATTCACGAAATATTCAAATGCATACATGCTGGTGTACATACGGGAAAGTGACAGAGATAAAATAATCTGCAATGTGGATGAAAAGGACATTGCTGAACATCTGAGGGAGAGGTTAAAGAAAGAACAAGAAGAAAAGGAGCACAAGAAGAAAGAAAAGGCAGAAGCACACCTGTATACTATTATAAAGGTTGCTCGGGATGAGAACCTCATGGAGCAAATTGGGAAGGATATTTATTTTGATCTTGTGGATCATGACAAAGTTAGAAGTTTTCGTATTCAGAAGCAGACTCCTTTTAACATTTTTAAGGAGGAGGTTGCAAAAGAGTTTGGCATACCAGTGCAATTTCAGCGTTTTTGGCTGTGGGCAAAACGTCAAAACCATACATATCGTCCTAACCGTCCATTGACGCCTATGGAGGAAACACAATCTGTTGGACAGTTGAGGGAGGTATCGAACAAGGTTCATAATGCAGAACTAAAGTTATTCTTAGAAGTAGAGCTTGGGCCGGATTTACACCATATTGCTCCACCTGACAAAACAAAGGATGATATCCTGCTTTTCTTTAAGCTTTATGATCCAGAAAAAGAAGAACTGCGATATGTTGGAAGACTTTTTGTGAAGAGCACTGGTAAGGCAGCTGAAATCTTGTCAAAATTAAATGAAATGGCTGGCTATGCTCCCGAGGAAGAGATTGACCTATATGAGGAAATCAAGTTTGAGCCCACTGTCATGTGTGAACCCATTGATAAGAAATTCACATTTCGAGCCTGCCAGCTTGAGGATGGAGACATTGTCTGCTTTCAGAAGCCAACTTCAGTTGAAATTGAACATCTCCGCTATCCTGATGTCCCATCTTTTCTGGAATATGTGCACAACCGTCAAGTTGTTCACTTCCGATCCCTTGAGAAACCCAAGGAAGATGATTTCTCCCTAGAACTGTCAAAACTTCATACCTATGATGATGTTGTGGAACGAGTAGCTCAACAACTTGGTTTGGACGATCCATCCAAACTTAGGCTTACACCGCACAACTGTTACTCACAACAACCCAAACCCCAGCCAATTAAGTATCGTGGGGTTGACCACTTGACTGATATGCTGGTCCACTACAATCAGACTTCAGATATACTGTACTATGAAGTGTTGGACATCCCTCTGCCTGAATTACAAGGTTTGAAAACTCTGAAAGTTGCATTCCATCTTGCTACTAAGGAAGAAGTGGTTATTCACACCATAAGACTGCCAAAACAGAGCACTGTGGGGGATGTAATTAATGATCTTAAAACTAAGGTTGAATTGTCTCATCCGGATGCAGAACTTAGGCTGCTTGAAGTTTTCTATCACAAGATTTACAAGGTTTTTCCTCACAGTGAAAAGATTGAGAACATAAACGATCAATACTGGACATTGCGAGCTGAAGAGGTTCCTGAGGAAGAGAAAAATCTTGGTCCTAATGATCGTGTAATTCATGTGTACCATTTCACTAAAGACACAGCTCAGAACCAAATGCAAATTCAAAACTTTGGGGAACCTTTTTTCTTGGTCATACATGAAGGCGAAACTTTGGCTGAAGTTAAAGCTCGAGTGCAAAAGAAATTACAGGTTGCAGATGAGGAGTTTGCTAAGTGGAAGTTTGCGTTTCTTTCCTTAGGTCGCCCAGAATATCTTCAGGACTCGGATATTGTGTCTAGTCGGTTTCAGAGAAGAGATGTCTATGGTGCTTGGGAGCAATATCTTGGGCTGGAGCACTCTGACAGTGCTCCTAAAAGATCTTATGCAGCTAATCAGAATCGTCATATGTTTGAAAAGCCAGTAAAAATTTACAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko05168 ko05169 ko05203 map04068 map05168 map05169 map05203
Pfam Domains
Protein Families
MATH UCH USP7_C2 USP7_ICP0_bdg

Protein Analysis

1118

Amino Acids

131.24

Weight (kDa)

5.59

Isoelectric Point (pI)

44.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MATH_2 PF22486 56 - 179 2.3e-38 MATH domain
MATH PF00917 61 - 180 1.4e-17 MATH domain
UCH PF00443 199 - 520 1.6e-58 Ubiquitin carboxyl-terminal hydrolase
UCH_1 PF13423 199 - 474 7.4e-14 Ubiquitin carboxyl-terminal hydrolase
USP7_ICP0_bdg PF12436 624 - 875 3.2e-78 ICP0-binding domain of Ubiquitin-specific protease 7
USP7_C2 PF14533 885 - 1095 1.4e-55 Ubiquitin-specific protease C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000333)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11910 AT3G11910 AT3G11910 AT3G11910 AT5G06600 AT5G06600 AT5G06600
fragaria_vesca FvH4_3g26930 FvH4_3g39132 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220
malus_domestica MD02G1261100.v1.1 MD03G1161100.v1.1 MD07G1059900.v1.1 MD11G1177100.v1.1 MD12G1047900.v1.1 MD12G1048000.v1.1 MD12G1048400.v1.1 MD12G1048500.v1.1 MD12G1048700.v1.1 MD12G1048800.v1.1 MD12G1056200.v1.1 MD14G1046300.v1.1 MD14G1046500.v1.1 MD15G1166900.v1.1
prunus_persica Prupe.2G066200_v2.0.a1 Prupe.2G066200_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1
pyrus_communis pycom02g22320 pycom03g11340 pycom07g04580 pycom11g14290 pycom11g15110 pycom12g04110 pycom12g04140 pycom12g04160 pycom12g04210 pycom12g04220 pycom12g04230 pycom12g04290 pycom12g04320 pycom12g04330 pycom12g04350 pycom12g04360 pycom14g03880 pycom14g03900 pycom16g18750
rosa_chinensis RchiOBHm_Chr1g0324111 RchiOBHm_Chr1g0324241 RchiOBHm_Chr3g0477691 RchiOBHm_Chr5g0049371 RchiOBHm_Chr5g0070531
rosa_laevigata RLG00000008612 RLG00000023698 RLG00000034613 RLG00000036139
rosa_multiflora Rmu_co8271779.1_g000001 Rmu_sc0006187.1_g000014
rosa_roxburghii Rroxscaffold_1G00010670 Rroxscaffold_1G00030450 Rroxscaffold_1G00031950 Rroxscaffold_1G00031970 Rroxscaffold_1G00032320 Rroxscaffold_1G00055490 Rroxscaffold_2G00083610 Rroxscaffold_2G00084480 Rroxscaffold_2G00115610 Rroxscaffold_3G00261320 Rroxscaffold_4G00312220 Rroxscaffold_4G00325820 Rroxscaffold_5G00339170 Rroxscaffold_5G00364030 Rroxscaffold_6G00403750
rosa_rugosa Rorug03G0162700 Rorug03G0162800 Rorug03G0162900 Rorug05G0247700 Rorug05G0406600 Rorug05G0406800
rosa_samantha Rh1AG060300 Rh3AG213000 Rh3BG246600 Rh3CG240400 Rh3DG239700 Rh5AG324400 Rh5AG462100 Rh5BG335500 Rh5BG480200 Rh5BG480300 Rh5CG360200 Rh5CG504500 Rh5CG504600 Rh5DG347800 Rh5DG492200 Rh5DG492300
rosa_wichuraiana Rw3G019270 Rw3G026910 Rw5G030650 Rw5G032610 Rw5G043040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1706
AasI GACNNNNNNGTC 1 cut(s) 776
AccB7I CCANNNNNTGG 2 cut(s) 94, 145
AccI GTMKAC 1 cut(s) 1697
AccII CGCG 2 cut(s) 255, 433
AccIII TCCGGA 1 cut(s) 2830
AciI CCGC 6 cut(s) 314, 433, 1201, 1487, 2359, 2549
AclWI GGATC 8 cut(s) 146, 159, 294, 479, 1779, 2093, 2415, 2519
AcsI RAATTY 6 cut(s) 381, 1409, 1523, 2276, 3036, 3342
AcuI CTGAAG 8 cut(s) 722, 992, 1308, 2322, 2628, 2961, 3114, 3176
AcyI GRCGYC 2 cut(s) 14, 1931
AfaI GTAC 7 cut(s) 402, 764, 846, 1190, 1559, 2657, 2999
AfiI CCNNNNNNNGG 9 cut(s) 64, 94, 145, 494, 495, 1380, 2227, 2302, 2538
AjnI CCWGG 1 cut(s) 1498
AjuI GAANNNNNNNTTGG 4 cut(s) 2007, 2039, 3021, 3053
AleI CACNNNNGTG 1 cut(s) 1211
Alw21I GWGCWC 7 cut(s) 1356, 1665, 2150, 2397, 2780, 3273, 3286
Alw26I GTCTC 3 cut(s) 578, 2304, 2829
Alw44I GTGCAC 2 cut(s) 1352, 2393
AlwI GGATC 8 cut(s) 146, 159, 294, 479, 1779, 2093, 2415, 2519
Ama87I CYCGRG 3 cut(s) 1716, 2204, 3105
Aor13HI TCCGGA 1 cut(s) 2830
AoxI GGCC 2 cut(s) 1382, 2027
ApaLI GTGCAC 2 cut(s) 1352, 2393
ApeKI GCWGC 5 cut(s) 240, 835, 2159, 2848, 3302
ApoI RAATTY 6 cut(s) 381, 1409, 1523, 2276, 3036, 3342
AseI ATTAAT 2 cut(s) 1221, 2796
Asp700I GAANNNNTTC 2 cut(s) 2946, 3189
AspLEI GCGC 2 cut(s) 255, 433
AspS9I GGNCC 3 cut(s) 2027, 2626, 2972
AsuHPI GGTGA 5 cut(s) 77, 1094, 1225, 1275, 1484
AvaI CYCGRG 3 cut(s) 1716, 2204, 3105
AvaII GGWCC 2 cut(s) 2626, 2972
AxyI CCTNAGG 2 cut(s) 2952, 3172
BaeGI GKGCMC 3 cut(s) 1356, 1453, 2397
BamHI GGATCC 1 cut(s) 151
BanII GRGCYC 1 cut(s) 2247
BbsI GAAGAC 1 cut(s) 2137
Bbv12I GWGCWC 7 cut(s) 1356, 1665, 2150, 2397, 2780, 3273, 3286
BbvI GCAGC 5 cut(s) 252, 847, 2171, 2835, 3314
BccI CCATC 6 cut(s) 166, 804, 2300, 2383, 2536, 2730
BceAI ACGGC 1 cut(s) 697
BciT130I CCWGG 1 cut(s) 1500
BclI TGATCA 1 cut(s) 28
BcoDI GTCTC 3 cut(s) 578, 2304, 2829
BfaI CTAG 5 cut(s) 195, 1454, 2456, 3213, 3352
BglII AGATCT 1 cut(s) 3293
BisI GCNGC 5 cut(s) 241, 836, 2160, 2849, 3303
BlsI GCNGC 5 cut(s) 242, 837, 2161, 2850, 3304
Bme1390I CCNGG 1 cut(s) 1500
Bme18I GGWCC 2 cut(s) 2626, 2972
BmeT110I CYCGRG 3 cut(s) 1716, 2204, 3105
BmgT120I GGNCC 3 cut(s) 2027, 2626, 2972
BmiI GGNNCC 3 cut(s) 153, 2949, 3054
BmrFI CCNGG 1 cut(s) 1500
BmrI ACTGGG 1 cut(s) 483
BmsI GCATC 3 cut(s) 39, 1111, 2824
BmuI ACTGGG 1 cut(s) 483
BpiI GAAGAC 1 cut(s) 2137
BpmI CTGGAG 3 cut(s) 626, 743, 3287
BpuEI CTTGAG 4 cut(s) 899, 1097, 2321, 2447
BsaHI GRCGYC 2 cut(s) 14, 1931
BsaJI CCNNGG 6 cut(s) 93, 531, 610, 1498, 2205, 2436
BsaWI WCCGGW 1 cut(s) 2830
BsaXI ACNNNNNCTCC 4 cut(s) 617, 647, 2301, 2331
Bsc4I CCNNNNNNNGG 9 cut(s) 64, 94, 145, 494, 495, 1380, 2227, 2302, 2538
Bse1I ACTGG 8 cut(s) 143, 489, 566, 760, 1859, 2155, 2930, 3335
Bse21I CCTNAGG 2 cut(s) 2952, 3172
Bse3DI GCAATG 3 cut(s) 1600, 1611, 2930
BseAI TCCGGA 1 cut(s) 2830
BseBI CCWGG 1 cut(s) 1500
BseDI CCNNGG 6 cut(s) 93, 531, 610, 1498, 2205, 2436
BseLI CCNNNNNNNGG 9 cut(s) 64, 94, 145, 494, 495, 1380, 2227, 2302, 2538
BseMI GCAATG 3 cut(s) 1600, 1611, 2930
BseMII CTCAG 5 cut(s) 484, 690, 1616, 2943, 3035
BseNI ACTGG 8 cut(s) 143, 489, 566, 760, 1859, 2155, 2930, 3335
BseRI GAGGAG 5 cut(s) 59, 629, 1472, 1847, 3152
BseSI GKGCMC 3 cut(s) 1356, 1453, 2397
BseXI GCAGC 5 cut(s) 252, 847, 2171, 2835, 3314
BseYI CCCAGC 1 cut(s) 2580
Bsh1236I CGCG 2 cut(s) 255, 433
BshFI GGCC 2 cut(s) 1384, 2029
BsiHKAI GWGCWC 7 cut(s) 1356, 1665, 2150, 2397, 2780, 3273, 3286
BsiHKCI CYCGRG 3 cut(s) 1716, 2204, 3105
BsiSI CCGG 2 cut(s) 2030, 2831
BslFI GGGAC 4 cut(s) 41, 827, 1217, 2357
BslI CCNNNNNNNGG 9 cut(s) 64, 94, 145, 494, 495, 1380, 2227, 2302, 2538
BsmAI GTCTC 3 cut(s) 578, 2304, 2829
BsmFI GGGAC 4 cut(s) 41, 827, 1217, 2357
BsmI GAATGC 1 cut(s) 2717
BsnI GGCC 2 cut(s) 1384, 2029
BsoBI CYCGRG 3 cut(s) 1716, 2204, 3105
Bsp1286I GDGCHC 9 cut(s) 1356, 1453, 1665, 2150, 2247, 2397, 2780, 3273, 3286
Bsp13I TCCGGA 1 cut(s) 2830
Bsp1407I TGTACA 2 cut(s) 844, 1557
Bsp19I CCATGG 1 cut(s) 93
BspACI CCGC 6 cut(s) 314, 433, 1201, 1487, 2359, 2549
BspANI GGCC 2 cut(s) 1384, 2029
BspCNI CTCAG 5 cut(s) 483, 691, 1617, 2944, 3034
BspEI TCCGGA 1 cut(s) 2830
BspFNI CGCG 2 cut(s) 255, 433
BspHI TCATGA 2 cut(s) 9, 1774
BspLI GGNNCC 3 cut(s) 153, 2949, 3054
BspPI GGATC 8 cut(s) 146, 159, 294, 479, 1779, 2093, 2415, 2519
BspQI GCTCTTC 2 cut(s) 741, 2138
BsrDI GCAATG 3 cut(s) 1600, 1611, 2930
BsrGI TGTACA 2 cut(s) 844, 1557
BsrI ACTGG 8 cut(s) 143, 489, 566, 760, 1859, 2155, 2930, 3335
BssECI CCNNGG 6 cut(s) 93, 531, 610, 1498, 2205, 2436
BssNAI GTATAC 1 cut(s) 1698
BssNI GRCGYC 2 cut(s) 14, 1931
BssT1I CCWWGG 3 cut(s) 93, 610, 2436
Bst1107I GTATAC 1 cut(s) 1698
Bst2UI CCWGG 1 cut(s) 1500
Bst6I CTCTTC 7 cut(s) 741, 1473, 2138, 2205, 2937, 2952, 3223
BstACI GRCGYC 2 cut(s) 14, 1931
BstAUI TGTACA 2 cut(s) 844, 1557
BstC8I GCNNGC 6 cut(s) 18, 257, 2194, 2293, 2297, 2937
BstDSI CCRYGG 1 cut(s) 93
BstENI CCTNNNNNAGG 2 cut(s) 1378, 2225
BstFNI CGCG 2 cut(s) 255, 433
BstHHI GCGC 2 cut(s) 255, 433
BstMAI GTCTC 3 cut(s) 578, 2304, 2829
BstMWI GCNNNNNNNGC 4 cut(s) 1870, 1879, 2198, 2548
BstNI CCWGG 1 cut(s) 1500
BstNSI RCATGY 1 cut(s) 1552
BstSCI CCNGG 1 cut(s) 1498
BstSLI GKGCMC 3 cut(s) 1356, 1453, 2397
BstUI CGCG 2 cut(s) 255, 433
BstV1I GCAGC 5 cut(s) 252, 847, 2171, 2835, 3314
BstV2I GAAGAC 1 cut(s) 2137
BstX2I RGATCY 2 cut(s) 151, 3293
BstXI CCANNNNNNTGG 2 cut(s) 304, 339
BstYI RGATCY 2 cut(s) 151, 3293
BstZ17I GTATAC 1 cut(s) 1698
Bsu36I CCTNAGG 2 cut(s) 2952, 3172
BsuRI GGCC 2 cut(s) 1384, 2029
BtgI CCRYGG 1 cut(s) 93
Cac8I GCNNGC 6 cut(s) 18, 257, 2194, 2293, 2297, 2937
CciI TCATGA 2 cut(s) 9, 1774
CfoI GCGC 2 cut(s) 255, 433
Cfr13I GGNCC 3 cut(s) 2027, 2626, 2972
CseI GACGC 2 cut(s) 22, 1939
Csp6I GTAC 7 cut(s) 401, 763, 845, 1189, 1558, 2656, 2998
CviQI GTAC 7 cut(s) 401, 763, 845, 1189, 1558, 2656, 2998
DrdI GACNNNNNNGTC 1 cut(s) 776
DseDI GACNNNNNNGTC 1 cut(s) 776
Eam1104I CTCTTC 7 cut(s) 741, 1473, 2138, 2205, 2937, 2952, 3223
EarI CTCTTC 7 cut(s) 741, 1473, 2138, 2205, 2937, 2952, 3223
Eco130I CCWWGG 3 cut(s) 93, 610, 2436
Eco24I GRGCYC 1 cut(s) 2247
Eco32I GATATC 1 cut(s) 2074
Eco47I GGWCC 2 cut(s) 2626, 2972
Eco57I CTGAAG 8 cut(s) 722, 992, 1308, 2322, 2628, 2961, 3114, 3176
Eco81I CCTNAGG 2 cut(s) 2952, 3172
Eco88I CYCGRG 3 cut(s) 1716, 2204, 3105
EcoNI CCTNNNNNAGG 2 cut(s) 1378, 2225
EcoRII CCWGG 1 cut(s) 1498
EcoRV GATATC 1 cut(s) 2074
EcoT14I CCWWGG 3 cut(s) 93, 610, 2436
EcoT22I ATGCAT 1 cut(s) 1546
EcoT38I GRGCYC 1 cut(s) 2247
ErhI CCWWGG 3 cut(s) 93, 610, 2436
FalI AAGNNNNNCTT 4 cut(s) 1994, 2026, 2007, 2039
FaqI GGGAC 4 cut(s) 41, 827, 1217, 2357
FauI CCCGC 2 cut(s) 426, 1194
FauNDI CATATG 6 cut(s) 334, 568, 687, 820, 942, 3321
FbaI TGATCA 1 cut(s) 28
FblI GTMKAC 1 cut(s) 1697
Fnu4HI GCNGC 5 cut(s) 241, 836, 2160, 2849, 3303
FriOI GRGCYC 1 cut(s) 2247
Fsp4HI GCNGC 5 cut(s) 241, 836, 2160, 2849, 3303
FspBI CTAG 5 cut(s) 195, 1454, 2456, 3213, 3352
GlaI GCGC 2 cut(s) 254, 432
GluI GCNGC 5 cut(s) 241, 836, 2160, 2849, 3303
GsaI CCCAGC 1 cut(s) 2584
GsuI CTGGAG 3 cut(s) 626, 743, 3287
HaeIII GGCC 2 cut(s) 1384, 2029
HapII CCGG 2 cut(s) 2030, 2831
HgaI GACGC 2 cut(s) 22, 1939
HhaI GCGC 2 cut(s) 255, 433
Hin1I GRCGYC 2 cut(s) 14, 1931
Hin6I GCGC 2 cut(s) 253, 431
HinP1I GCGC 2 cut(s) 253, 431
HincII GTYRAC 1 cut(s) 2605
HindII GTYRAC 1 cut(s) 2605
HindIII AAGCTT 1 cut(s) 2090
HinfI GANTC 9 cut(s) 168, 317, 572, 742, 824, 992, 1810, 3197, 3313
HpaII CCGG 2 cut(s) 2030, 2831
HphI GGTGA 5 cut(s) 77, 1094, 1225, 1275, 1484
HpyAV CCTTC 6 cut(s) 886, 926, 1016, 1123, 1741, 3071
HpyCH4IV ACGT 5 cut(s) 620, 966, 1017, 1072, 1894
HpyF10VI GCNNNNNNNGC 4 cut(s) 1870, 1879, 2198, 2548
HpySE526I ACGT 5 cut(s) 620, 966, 1017, 1072, 1894
Hsp92I GRCGYC 2 cut(s) 14, 1931
HspAI GCGC 2 cut(s) 253, 431
Kpn2I TCCGGA 1 cut(s) 2830
Ksp22I TGATCA 1 cut(s) 28
LguI GCTCTTC 2 cut(s) 741, 2138
Lsp1109I GCAGC 5 cut(s) 252, 847, 2171, 2835, 3314
LweI GCATC 3 cut(s) 39, 1111, 2824
MaeI CTAG 5 cut(s) 195, 1454, 2456, 3213, 3352
MaeII ACGT 5 cut(s) 620, 966, 1017, 1072, 1894
MaeIII GTNAC 6 cut(s) 714, 767, 1426, 1482, 1571, 2558
MflI RGATCY 2 cut(s) 151, 3293
MhlI GDGCHC 9 cut(s) 1356, 1453, 1665, 2150, 2247, 2397, 2780, 3273, 3286
MlyI GAGTC 4 cut(s) 566, 751, 1804, 3191
MmeI TCCRAC 3 cut(s) 1936, 2107, 2649
Mph1103I ATGCAT 1 cut(s) 1546
MroI TCCGGA 1 cut(s) 2830
MroXI GAANNNNTTC 2 cut(s) 2946, 3189
MslI CAYNNNNRTG 9 cut(s) 337, 1211, 1344, 1368, 1553, 1859, 2252, 2478, 2832
MspA1I CMGCKG 1 cut(s) 2162
MspI CCGG 2 cut(s) 2030, 2831
MspR9I CCNGG 1 cut(s) 1500
Mva1269I GAATGC 1 cut(s) 2717
MvaI CCWGG 1 cut(s) 1500
MvnI CGCG 2 cut(s) 255, 433
MwoI GCNNNNNNNGC 4 cut(s) 1870, 1879, 2198, 2548
NcoI CCATGG 1 cut(s) 93
NdeI CATATG 6 cut(s) 334, 568, 687, 820, 942, 3321
NlaIV GGNNCC 3 cut(s) 153, 2949, 3054
NmeAIII GCCGAG 1 cut(s) 556
NmuCI GTSAC 3 cut(s) 714, 767, 1571
NsiI ATGCAT 1 cut(s) 1546
NspI RCATGY 1 cut(s) 1552
OliI CACNNNNGTG 1 cut(s) 1211
PaeR7I CTCGAG 1 cut(s) 3105
PagI TCATGA 2 cut(s) 9, 1774
PciSI GCTCTTC 2 cut(s) 741, 2138
PcsI WCGNNNNNNNCGW 1 cut(s) 1918
PctI GAATGC 1 cut(s) 2717
PdmI GAANNNNTTC 2 cut(s) 2946, 3189
PfeI GAWTC 5 cut(s) 168, 317, 824, 992, 3313
PflFI GACNNNGTC 2 cut(s) 799, 2315
PflMI CCANNNNNTGG 2 cut(s) 94, 145
PkrI GCNGC 5 cut(s) 242, 837, 2161, 2850, 3304
PleI GAGTC 4 cut(s) 566, 750, 1804, 3191
PpsI GAGTC 4 cut(s) 566, 750, 1804, 3191
PshBI ATTAAT 2 cut(s) 1221, 2796
PsiI TTATAA 1 cut(s) 1706
Psp6I CCWGG 1 cut(s) 1498
PspFI CCCAGC 1 cut(s) 2580
PspGI CCWGG 1 cut(s) 1498
PspN4I GGNNCC 3 cut(s) 153, 2949, 3054
PspPI GGNCC 3 cut(s) 2027, 2626, 2972
PspXI VCTCGAGB 1 cut(s) 3105
PsuI RGATCY 2 cut(s) 151, 3293
PsyI GACNNNGTC 2 cut(s) 799, 2315
PvuII CAGCTG 1 cut(s) 2162
RsaI GTAC 7 cut(s) 402, 764, 846, 1190, 1559, 2657, 2999
RsaNI GTAC 7 cut(s) 401, 763, 845, 1189, 1558, 2656, 2998
RseI CAYNNNNRTG 9 cut(s) 337, 1211, 1344, 1368, 1553, 1859, 2252, 2478, 2832
SapI GCTCTTC 2 cut(s) 741, 2138
SatI GCNGC 5 cut(s) 241, 836, 2160, 2849, 3303
Sau96I GGNCC 3 cut(s) 2027, 2626, 2972
SchI GAGTC 4 cut(s) 566, 751, 1804, 3191
ScrFI CCNGG 1 cut(s) 1500
SduI GDGCHC 9 cut(s) 1356, 1453, 1665, 2150, 2247, 2397, 2780, 3273, 3286
SfaNI GCATC 3 cut(s) 39, 1111, 2824
Sfr274I CTCGAG 1 cut(s) 3105
SinI GGWCC 2 cut(s) 2626, 2972
SlaI CTCGAG 1 cut(s) 3105
SmiMI CAYNNNNRTG 9 cut(s) 337, 1211, 1344, 1368, 1553, 1859, 2252, 2478, 2832
SmlI CTYRAG 5 cut(s) 878, 1076, 2300, 2426, 3105
SmoI CTYRAG 5 cut(s) 878, 1076, 2300, 2426, 3105
SsiI CCGC 6 cut(s) 314, 433, 1201, 1487, 2359, 2549
SspI AATATT 2 cut(s) 1277, 1535
SspMI CTAG 5 cut(s) 195, 1454, 2456, 3213, 3352
StyD4I CCNGG 1 cut(s) 1498
StyI CCWWGG 3 cut(s) 93, 610, 2436
TaiI ACGT 5 cut(s) 623, 969, 1020, 1075, 1897
TaqI TCGA 4 cut(s) 1261, 1976, 2287, 3106
TatI WGTACW 4 cut(s) 762, 844, 1557, 2655
TfiI GAWTC 5 cut(s) 168, 317, 824, 992, 3313
TseFI GTSAC 3 cut(s) 714, 767, 1571
TseI GCWGC 5 cut(s) 240, 835, 2159, 2848, 3302
Tsp45I GTSAC 3 cut(s) 714, 767, 1571
Tth111I GACNNNGTC 2 cut(s) 799, 2315
Van91I CCANNNNNTGG 2 cut(s) 94, 145
VneI GTGCAC 2 cut(s) 1352, 2393
VpaK11BI GGWCC 2 cut(s) 2626, 2972
VspI ATTAAT 2 cut(s) 1221, 2796
XagI CCTNNNNNAGG 2 cut(s) 1378, 2225
XapI RAATTY 6 cut(s) 381, 1409, 1523, 2276, 3036, 3342
XceI RCATGY 1 cut(s) 1552
XcmI CCANNNNNNNNNTGG 1 cut(s) 2303
XhoI CTCGAG 1 cut(s) 3105
XmiI GTMKAC 1 cut(s) 1697
XmnI GAANNNNTTC 2 cut(s) 2946, 3189
XspI CTAG 5 cut(s) 195, 1454, 2456, 3213, 3352
Zsp2I ATGCAT 1 cut(s) 1546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.