RchiOBHm_Chr5g0049371

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000333
Physical Location & Seq
Reverse (-)
46901256 .. 46915736
14481 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32704

Sequence Viewer

Length: 3357 bp
ATGACTGTCATGACCCCTGCCCCTATTGATCAACCAGAGGACGAGGAGATGCTTGTTCCAGCCTCGGATTTGCCTGAAAACAATCACCAGCCCATGGATGTTGTTGCACAGCCTGAAAATGCTAATAATGTGGAGAACCAACCAGTAGAAGATCCTCCTTCATCGAGATTCACATGGAGAATTGACAACTTTTCTAGGATGAATACAAAGAAGCTTTATTCTGACATATTTGTTGTTGGTGGCTATAAATGGCGTGTGCTTATTTTTCCCAAGGGAAACAATGTGGATCATTTGTCTATGTATTTGGATGTTGCGGATTCGTCAAATTTGCCATATGGGTGGAGTAGATATGCTCAATTTAGCTTGGGAGTTCTCAATCAACTTCATACCAAGTATTCTGTACGAAAAGACACACAACATCAGTTCAATGCACGGGAAAGTGATTGGGGTTTCACATCCTTTATGCCTCTTGGTGAACTGTACGATCCCAGCAGAGGTTATCTCGTGAATGATGCGGTTATAATTGAAGCGGAAGTTCTCGTTCGTAGGGTTGTAGATTATTGGACATATGACTCAAAGAAGGAAACGGGGTATGTTGGGCTTAAGAACCAAGGAGCAACATGCTATATGAACTCTCTCCTGCAGACTCTGTATCATATTCCTTATTTTAGAAAGGCTGTTTACCATATGCCCACGACTGAGAATGATTTGCCATCACAAAGCATACCATTGGCTCTTCAGAGTCTATTTTACAAACTCCAGTACAGTGATAATAGTGTTGCAACAAAAGAGCTGACAAAGTCTTTTGGATGGGATACATATGATTCATTTATGCAGCATGATGTGCAAGAACTAAATAGGGTCTTGTGTGAAAAACTGGAGGACAAAATGAAGGGAACTGTTGTAGAGGGAACCATACAGAAGTTATTTGAAGGTCACCATATGAACTACATCGAATGCATTAATGTGGACTACAAATCTACAAGAAAGGAGTCATTTTATGACCTTCAGCTCGATGTCAAAGGGTGTCGTGATGTATATGCTTCTTTTGATAAGTATGTAGAAGTTGAACGTCTTGAGGGGGATAACAAGTATCATGCTGAACAGAATGGATTACAGGATGCTAAGAAAGGGGTTCTCTTCATTGACTTTCCACCTGTTCTTCAACTTCAACTGAAACGGTTTGAGTACGACTTTATGCGAGACACCATGGTGAAGATTAATGACCGTTATGAGTTCCCTTTGCAACTTGACCTTGATCGAGATAATGGAAAATATTTATCACCTGAAGCTGATAGGAGTGTTCGCAACCTTTACACACTTCACAGTGTATTGGTACACAGTGGTGGGGTGCATGGTGGACATTACTACGCCTTTATCAGACCGACGCTTTCTGATCAGTGGTACAAATTTGATGATGAGAGGGTAACAAAAGAAGATATAAAGAGGGCACTGGAGGAGCAGTATGGTGGTGAGGAAGAGTTACCGCAGACAAACCCTGGATTTAATAACACTCCTTTCAAATTTACAAAATACTCAAACGCGTACATGCTGGTGTACATACGTGAAAGTGACAAGGATAAAATAATCTGCAATGTTGATGAGAAGGACATTGCTGAACATCTTCGGGAGAGGTTGAAGAAAGAACAAGAAGAAAAGGAGCACAAGAAGAAAGAAAAGGCAGAGGCACACCTATATACTATCATTAAGGTTGCCCGGGACGAGAACCTTCTGGAGCAGATTGGGAAGGATATATATTTTGATCTTGTGGATCATGACAAAGTCAAAAGTTTTCGTATTCAGAAGCAGATGCCTTTCAACCTTTTCAAGGAGGAGGTTGCTAAGGAGTTTGGCATACCAGTGCAATTTCAGCGGTTTTGGCTGTGGGCAAAGCGTCAAAACCATACATATCGTCCAAACCGTCCATTGACACCTGTGGAGGAAACACAATCTGTTGGGCAGTTGAGGGAGGTATCAAACAAGGTTCATAATGCAGAACTGAAGTTGTTCTTGGAAATAGAGCTTGGACCGGATTTACACCCTATTGCTCCACCTGACAAGACAAAGGATGATATTCTGCTTTTCTTTAAGCTTTATGATCCTGAAAAAGAAGAACTACGTTATGTTGGACGGCTTTTTGTGAAGAGTACTGGTAAGCCAGCTGAAATCTTGTCAAAATTAAATGAATTGGCTGGCTATGCCCCTGATGAGGAGATCGACCTTTACGAGGAAATTAAGTATGAGCCCACTGTCATGTGTGAACCCATCGATAAGAAATTCACATTTCGAGCCAGCCAGCTTGAGGATGGAGACATTGTTTGCTTCCAAAAACCAGTTCCGGTTGAAAATGGTCAGCAGTTCCGCTATCCAGACGTCCCATCTTTTCTGGATTTTGTGCACAATCGTCTCGTTGTTCATTTTCGATCGTTTGAGAAACCCAAAGAAGATGATTTCAGCTTAGAATTGTCAAAACTTCATACCTATGATGATGTTGTGGAGCGAGTAGCTCAACAACTTGGTTTGGACGATCCATCCAAAATTAGGCTTACCTCACATAATTGTTACTCCCAGCAACCCAAACCTCAGCCAATTAAGTATCGGGGGGTAGACCGTTTGACTGATATGCTTGTTCACTACAATCAGACTTCGGATATATTGTACTATGAAGTATTAGACATCCCTCTGCCTGAATTACAAGGTTTGAAAACCCTGAAAGTTGCATTCCATCATGCTACCAAGGATGAAGTGGTTATTCACACCATAAGACTGCCAAAACAGAGCACTGTTGGGGATGTAATCAATGATCTTAAAACTAAGGTTGAATTGTCTCATCCAGATGCAGAACTTAGGTTGCTCGAGGTTTTCTATCACAAGATATACAAGGTCTTTCCCCAAAGTGAAAAGATCGAAAACATAAATGATCAATACTGGACGTTACGTGCTGAAGAGGTTCCAGAGGAAGAGAAAAATCTTGGTCCTAATGACCGTGTAATTCATGTCTACCACTTCACTAAAGACACCGCTCAGAATCAAATGCAAATTCAAAACTTTGGGGAACCGTTTTTCTTGGTTATACATGAAGGTGAGACCTTGGCTGAAATTAGAGGTCGGATACAAAAGAAATTACAGGTTGCAGATGAGGAATTTGCGAAGTGGAAGTTTGCATTTCTTTCGCTAGGTCGGCCAGAGTATCTTCAGGACACTGACATCGTCTCTAGTCGGTTCCAGAGAAGAGATGTTTATGGTGCTTGGGAGCAGTATCTTGGCCTGGAGCACTCTGACAGCGCTCCTAAAAGAGCTTATGCAGCTAATCAGAATCGCCACACATTTGAGAAGCCGGTGAAAATTTACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko05168 ko05169 ko05203 map04068 map05168 map05169 map05203
Pfam Domains
Protein Families
MATH UCH USP7_C2 USP7_ICP0_bdg

Protein Analysis

1118

Amino Acids

131.0

Weight (kDa)

5.52

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MATH_2 PF22486 56 - 179 5.2e-39 MATH domain
MATH PF00917 61 - 180 1.5e-17 MATH domain
UCH PF00443 199 - 520 1.6e-59 Ubiquitin carboxyl-terminal hydrolase
UCH_1 PF13423 199 - 474 2e-15 Ubiquitin carboxyl-terminal hydrolase
USP7_ICP0_bdg PF12436 624 - 876 3.9e-78 ICP0-binding domain of Ubiquitin-specific protease 7
USP7_C2 PF14533 886 - 1096 1.1e-55 Ubiquitin-specific protease C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000333)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11910 AT3G11910 AT3G11910 AT3G11910 AT5G06600 AT5G06600 AT5G06600
fragaria_vesca FvH4_3g26930 FvH4_3g39132 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220
malus_domestica MD02G1261100.v1.1 MD03G1161100.v1.1 MD07G1059900.v1.1 MD11G1177100.v1.1 MD12G1047900.v1.1 MD12G1048000.v1.1 MD12G1048400.v1.1 MD12G1048500.v1.1 MD12G1048700.v1.1 MD12G1048800.v1.1 MD12G1056200.v1.1 MD14G1046300.v1.1 MD14G1046500.v1.1 MD15G1166900.v1.1
prunus_persica Prupe.2G066200_v2.0.a1 Prupe.2G066200_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1
pyrus_communis pycom02g22320 pycom03g11340 pycom07g04580 pycom11g14290 pycom11g15110 pycom12g04110 pycom12g04140 pycom12g04160 pycom12g04210 pycom12g04220 pycom12g04230 pycom12g04290 pycom12g04320 pycom12g04330 pycom12g04350 pycom12g04360 pycom14g03880 pycom14g03900 pycom16g18750
rosa_chinensis RchiOBHm_Chr1g0324111 RchiOBHm_Chr1g0324241 RchiOBHm_Chr3g0477691 RchiOBHm_Chr5g0049371 RchiOBHm_Chr5g0070531
rosa_laevigata RLG00000008612 RLG00000023698 RLG00000034613 RLG00000036139
rosa_multiflora Rmu_co8271779.1_g000001 Rmu_sc0006187.1_g000014
rosa_roxburghii Rroxscaffold_1G00010670 Rroxscaffold_1G00030450 Rroxscaffold_1G00031950 Rroxscaffold_1G00031970 Rroxscaffold_1G00032320 Rroxscaffold_1G00055490 Rroxscaffold_2G00083610 Rroxscaffold_2G00084480 Rroxscaffold_2G00115610 Rroxscaffold_3G00261320 Rroxscaffold_4G00312220 Rroxscaffold_4G00325820 Rroxscaffold_5G00339170 Rroxscaffold_5G00364030 Rroxscaffold_6G00403750
rosa_rugosa Rorug03G0162700 Rorug03G0162800 Rorug03G0162900 Rorug05G0247700 Rorug05G0406600 Rorug05G0406800
rosa_samantha Rh1AG060300 Rh3AG213000 Rh3BG246600 Rh3CG240400 Rh3DG239700 Rh5AG324400 Rh5AG462100 Rh5BG335500 Rh5BG480200 Rh5BG480300 Rh5CG360200 Rh5CG504500 Rh5CG504600 Rh5DG347800 Rh5DG492200 Rh5DG492300
rosa_wichuraiana Rw3G019270 Rw3G026910 Rw5G030650 Rw5G032610 Rw5G043040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 521
AatII GACGTC 1 cut(s) 2376
AccB7I CCANNNNNTGG 1 cut(s) 94
AccBSI CCGCTC 1 cut(s) 3023
AccI GTMKAC 2 cut(s) 2607, 3000
AccII CGCG 1 cut(s) 1544
AciI CCGC 7 cut(s) 314, 515, 530, 1487, 1873, 2362, 3021
AclWI GGATC 6 cut(s) 146, 294, 479, 1779, 2093, 2522
AcoI YGGCCR 1 cut(s) 3182
AcsI RAATTY 7 cut(s) 325, 1409, 1523, 2276, 3039, 3143, 3345
AcuI CTGAAG 6 cut(s) 722, 992, 1308, 2021, 2964, 3179
AcyI GRCGYC 1 cut(s) 2373
AfeI AGCGCT 1 cut(s) 3286
AfiI CCNNNNNNNGG 7 cut(s) 94, 494, 1828, 2209, 2227, 2302, 2541
AflII CTTAAG 1 cut(s) 602
AflIII ACRYGT 1 cut(s) 1542
AjnI CCWGG 2 cut(s) 1498, 3267
AjuI GAANNNNNNNTTGG 6 cut(s) 1994, 2007, 2026, 2039, 2319, 2351
AleI CACNNNNGTG 2 cut(s) 1211, 1344
Alw21I GWGCWC 4 cut(s) 1665, 2400, 2783, 3276
Alw26I GTCTC 6 cut(s) 1197, 2304, 2411, 2832, 3080, 3217
Alw44I GTGCAC 1 cut(s) 2396
AlwI GGATC 6 cut(s) 146, 294, 479, 1779, 2093, 2522
AlwNI CAGNNNCTG 1 cut(s) 649
Ama87I CYCGRG 2 cut(s) 1716, 2855
Aor51HI AGCGCT 1 cut(s) 3286
AoxI GGCC 2 cut(s) 3182, 3265
ApaLI GTGCAC 1 cut(s) 2396
ApeKI GCWGC 2 cut(s) 835, 3305
ApoI RAATTY 7 cut(s) 325, 1409, 1523, 2276, 3039, 3143, 3345
AseI ATTAAT 2 cut(s) 963, 1221
Asp700I GAANNNNTTC 3 cut(s) 1623, 2006, 2949
AspLEI GCGC 1 cut(s) 3287
AspS9I GGNCC 2 cut(s) 2027, 2975
AsuC2I CCSGG 2 cut(s) 1717, 1718
AsuHPI GGTGA 8 cut(s) 77, 485, 929, 1225, 1275, 1484, 3095, 3352
AvaI CYCGRG 2 cut(s) 1716, 2855
AvaII GGWCC 2 cut(s) 2027, 2975
BaeGI GKGCMC 2 cut(s) 1453, 2400
BanII GRGCYC 1 cut(s) 2247
BauI CACGAG 1 cut(s) 503
Bbv12I GWGCWC 4 cut(s) 1665, 2400, 2783, 3276
BbvCI CCTCAGC 1 cut(s) 2583
BbvI GCAGC 2 cut(s) 847, 3317
BccI CCATC 7 cut(s) 721, 804, 2273, 2300, 2386, 2539, 2733
BceAI ACGGC 1 cut(s) 2147
BcgI CGANNNNNNTGC 2 cut(s) 3153, 3187
BciT130I CCWGG 2 cut(s) 1500, 3269
BciVI GTATCC 2 cut(s) 808, 3105
BclI TGATCA 3 cut(s) 28, 1396, 2920
BcnI CCSGG 2 cut(s) 1717, 1718
BcoDI GTCTC 6 cut(s) 1197, 2304, 2411, 2832, 3080, 3217
BfaI CTAG 3 cut(s) 195, 3176, 3216
BfmI CTRYAG 1 cut(s) 641
BfoI RGCGCY 1 cut(s) 3288
BfrI CTTAAG 1 cut(s) 602
BfuI GTATCC 2 cut(s) 808, 3105
BisI GCNGC 2 cut(s) 836, 3306
BlsI GCNGC 2 cut(s) 837, 3307
BmcAI AGTACT 1 cut(s) 2149
Bme1390I CCNGG 4 cut(s) 1500, 1717, 1718, 3269
Bme18I GGWCC 2 cut(s) 2027, 2975
BmeT110I CYCGRG 2 cut(s) 1716, 2855
BmgT120I GGNCC 2 cut(s) 2027, 2975
BmiI GGNNCC 4 cut(s) 913, 2952, 3057, 3224
BmrFI CCNGG 4 cut(s) 1500, 1717, 1718, 3269
BmsI GCATC 5 cut(s) 39, 502, 1111, 1800, 2827
BplI GAGNNNNNCTC 2 cut(s) 486, 518
BpmI CTGGAG 5 cut(s) 743, 899, 1475, 1754, 3290
Bpu10I CCTNAGC 2 cut(s) 1842, 2583
BpuEI CTTGAG 2 cut(s) 1097, 2321
BpuMI CCSGG 2 cut(s) 1717, 1718
Bsa29I ATCGAT 1 cut(s) 2268
BsaAI YACGTR 2 cut(s) 1565, 2939
BsaHI GRCGYC 1 cut(s) 2373
BsaI GGTCTC 1 cut(s) 3080
BsaJI CCNNGG 9 cut(s) 63, 93, 270, 610, 1209, 1498, 1716, 2736, 3090
BsaWI WCCGGW 2 cut(s) 2029, 2338
BsaXI ACNNNNNCTCC 4 cut(s) 1448, 1478, 2301, 2331
Bsc4I CCNNNNNNNGG 7 cut(s) 94, 494, 1828, 2209, 2227, 2302, 2541
Bse118I RCCGGY 1 cut(s) 3337
Bse1I ACTGG 8 cut(s) 143, 760, 882, 1458, 1859, 2155, 2333, 2933
Bse3DI GCAATG 2 cut(s) 1600, 1611
BseBI CCWGG 2 cut(s) 1500, 3269
BseCI ATCGAT 1 cut(s) 2268
BseDI CCNNGG 9 cut(s) 63, 93, 270, 610, 1209, 1498, 1716, 2736, 3090
BseLI CCNNNNNNNGG 7 cut(s) 94, 494, 1828, 2209, 2227, 2302, 2541
BseMI GCAATG 2 cut(s) 1600, 1611
BseMII CTCAG 3 cut(s) 690, 2597, 3038
BseNI ACTGG 8 cut(s) 143, 760, 882, 1458, 1859, 2155, 2333, 2933
BseRI GAGGAG 4 cut(s) 59, 1472, 1847, 2225
BseSI GKGCMC 2 cut(s) 1453, 2400
BseXI GCAGC 2 cut(s) 847, 3317
BseYI CCCAGC 2 cut(s) 488, 2568
Bsh1236I CGCG 1 cut(s) 1544
Bsh1285I CGRYCG 1 cut(s) 2426
BshFI GGCC 2 cut(s) 3184, 3267
BshVI ATCGAT 1 cut(s) 2268
BsiEI CGRYCG 1 cut(s) 2426
BsiHKAI GWGCWC 4 cut(s) 1665, 2400, 2783, 3276
BsiHKCI CYCGRG 2 cut(s) 1716, 2855
BsiSI CCGG 4 cut(s) 1717, 2030, 2339, 3338
BslFI GGGAC 2 cut(s) 1733, 2360
BslI CCNNNNNNNGG 7 cut(s) 94, 494, 1828, 2209, 2227, 2302, 2541
BsmAI GTCTC 6 cut(s) 1197, 2304, 2411, 2832, 3080, 3217
BsmBI CGTCTC 2 cut(s) 2411, 3217
BsmFI GGGAC 2 cut(s) 1733, 2360
BsmI GAATGC 2 cut(s) 962, 2720
BsnI GGCC 2 cut(s) 3184, 3267
Bso31I GGTCTC 1 cut(s) 3080
BsoBI CYCGRG 2 cut(s) 1716, 2855
Bsp1286I GDGCHC 6 cut(s) 1453, 1665, 2247, 2400, 2783, 3276
Bsp1407I TGTACA 1 cut(s) 1557
Bsp19I CCATGG 2 cut(s) 93, 1209
BspACI CCGC 7 cut(s) 314, 515, 530, 1487, 1873, 2362, 3021
BspANI GGCC 2 cut(s) 3184, 3267
BspCNI CTCAG 3 cut(s) 691, 2596, 3037
BspDI ATCGAT 1 cut(s) 2268
BspFNI CGCG 1 cut(s) 1544
BspHI TCATGA 2 cut(s) 9, 1774
BspLI GGNNCC 4 cut(s) 913, 2952, 3057, 3224
BspMAI CTGCAG 1 cut(s) 645
BspPI GGATC 6 cut(s) 146, 294, 479, 1779, 2093, 2522
BspQI GCTCTTC 1 cut(s) 741
BspTI CTTAAG 1 cut(s) 602
BspTNI GGTCTC 1 cut(s) 3080
BsrBI CCGCTC 1 cut(s) 3023
BsrDI GCAATG 2 cut(s) 1600, 1611
BsrFI RCCGGY 1 cut(s) 3337
BsrGI TGTACA 1 cut(s) 1557
BsrI ACTGG 8 cut(s) 143, 760, 882, 1458, 1859, 2155, 2333, 2933
BssAI RCCGGY 1 cut(s) 3337
BssECI CCNNGG 9 cut(s) 63, 93, 270, 610, 1209, 1498, 1716, 2736, 3090
BssNI GRCGYC 1 cut(s) 2373
BssSI CACGAG 1 cut(s) 503
BssT1I CCWWGG 6 cut(s) 93, 270, 610, 1209, 2736, 3090
Bst2BI CACGAG 1 cut(s) 503
Bst2UI CCWGG 2 cut(s) 1500, 3269
Bst6I CTCTTC 7 cut(s) 741, 1145, 1473, 2138, 2940, 2955, 3226
BstACI GRCGYC 1 cut(s) 2373
BstAFI CTTAAG 1 cut(s) 602
BstAPI GCANNNNNTGC 1 cut(s) 844
BstAUI TGTACA 1 cut(s) 1557
BstBAI YACGTR 2 cut(s) 1565, 2939
BstC8I GCNNGC 4 cut(s) 2160, 2194, 2293, 2297
BstDEI CTNAG 8 cut(s) 699, 1125, 1842, 2458, 2583, 2814, 2846, 3024
BstDSI CCRYGG 2 cut(s) 93, 1209
BstEII GGTNACC 1 cut(s) 935
BstENI CCTNNNNNAGG 2 cut(s) 1826, 2225
BstFNI CGCG 1 cut(s) 1544
BstH2I RGCGCY 1 cut(s) 3288
BstHHI GCGC 1 cut(s) 3287
BstMAI GTCTC 6 cut(s) 1197, 2304, 2411, 2832, 3080, 3217
BstMCI CGRYCG 1 cut(s) 2426
BstMWI GCNNNNNNNGC 6 cut(s) 844, 1870, 1879, 2198, 3181, 3305
BstNI CCWGG 2 cut(s) 1500, 3269
BstNSI RCATGY 2 cut(s) 624, 1552
BstPI GGTNACC 1 cut(s) 935
BstSCI CCNGG 4 cut(s) 1498, 1715, 1716, 3267
BstSFI CTRYAG 1 cut(s) 641
BstSLI GKGCMC 2 cut(s) 1453, 2400
BstUI CGCG 1 cut(s) 1544
BstV1I GCAGC 2 cut(s) 847, 3317
BstX2I RGATCY 1 cut(s) 151
BstXI CCANNNNNNTGG 1 cut(s) 339
BstYI RGATCY 1 cut(s) 151
Bsu15I ATCGAT 1 cut(s) 2268
BsuI GTATCC 2 cut(s) 808, 3105
BsuRI GGCC 2 cut(s) 3184, 3267
BsuTUI ATCGAT 1 cut(s) 2268
BtgI CCRYGG 2 cut(s) 93, 1209
BtsIMutI CAGTG 9 cut(s) 772, 1333, 1348, 1406, 1451, 1866, 2247, 2781, 3201
Cac8I GCNNGC 4 cut(s) 2160, 2194, 2293, 2297
CaiI CAGNNNCTG 1 cut(s) 649
CciI TCATGA 2 cut(s) 9, 1774
CfoI GCGC 1 cut(s) 3287
Cfr10I RCCGGY 1 cut(s) 3337
Cfr13I GGNCC 2 cut(s) 2027, 2975
Cfr9I CCCGGG 1 cut(s) 1716
ClaI ATCGAT 1 cut(s) 2268
CseI GACGC 2 cut(s) 1396, 1883
DdeI CTNAG 8 cut(s) 699, 1125, 1842, 2458, 2583, 2814, 2846, 3024
EaeI YGGCCR 1 cut(s) 3182
Eam1104I CTCTTC 7 cut(s) 741, 1145, 1473, 2138, 2940, 2955, 3226
EarI CTCTTC 7 cut(s) 741, 1145, 1473, 2138, 2940, 2955, 3226
Eco130I CCWWGG 6 cut(s) 93, 270, 610, 1209, 2736, 3090
Eco24I GRGCYC 1 cut(s) 2247
Eco31I GGTCTC 1 cut(s) 3080
Eco47I GGWCC 2 cut(s) 2027, 2975
Eco47III AGCGCT 1 cut(s) 3286
Eco57I CTGAAG 6 cut(s) 722, 992, 1308, 2021, 2964, 3179
Eco88I CYCGRG 2 cut(s) 1716, 2855
Eco91I GGTNACC 1 cut(s) 935
EcoNI CCTNNNNNAGG 2 cut(s) 1826, 2225
EcoO65I GGTNACC 1 cut(s) 935
EcoRII CCWGG 2 cut(s) 1498, 3267
EcoT14I CCWWGG 6 cut(s) 93, 270, 610, 1209, 2736, 3090
EcoT22I ATGCAT 1 cut(s) 962
EcoT38I GRGCYC 1 cut(s) 2247
ErhI CCWWGG 6 cut(s) 93, 270, 610, 1209, 2736, 3090
Esp3I CGTCTC 2 cut(s) 2411, 3217
FalI AAGNNNNNCTT 2 cut(s) 1994, 2026
FaqI GGGAC 2 cut(s) 1733, 2360
FauNDI CATATG 5 cut(s) 334, 568, 687, 820, 942
FbaI TGATCA 3 cut(s) 28, 1396, 2920
FblI GTMKAC 2 cut(s) 2607, 3000
Fnu4HI GCNGC 2 cut(s) 836, 3306
FriOI GRGCYC 1 cut(s) 2247
Fsp4HI GCNGC 2 cut(s) 836, 3306
FspBI CTAG 3 cut(s) 195, 3176, 3216
GlaI GCGC 1 cut(s) 3286
GluI GCNGC 2 cut(s) 836, 3306
GsaI CCCAGC 2 cut(s) 492, 2572
GsuI CTGGAG 5 cut(s) 743, 899, 1475, 1754, 3290
HaeII RGCGCY 1 cut(s) 3288
HaeIII GGCC 2 cut(s) 3184, 3267
HapII CCGG 4 cut(s) 1717, 2030, 2339, 3338
HgaI GACGC 2 cut(s) 1396, 1883
HhaI GCGC 1 cut(s) 3287
Hin1I GRCGYC 1 cut(s) 2373
Hin6I GCGC 1 cut(s) 3285
HinP1I GCGC 1 cut(s) 3285
HindIII AAGCTT 2 cut(s) 212, 2090
HinfI GANTC 9 cut(s) 168, 317, 572, 646, 742, 824, 992, 3028, 3316
HpaII CCGG 4 cut(s) 1717, 2030, 2339, 3338
HphI GGTGA 8 cut(s) 77, 485, 929, 1225, 1275, 1484, 3095, 3352
Hpy99I CGWCG 1 cut(s) 1390
HpyAV CCTTC 9 cut(s) 168, 574, 886, 926, 1016, 1600, 1739, 1741, 3074
HpyCH4IV ACGT 6 cut(s) 1072, 1564, 2119, 2373, 2933, 2938
HpyF10VI GCNNNNNNNGC 6 cut(s) 844, 1870, 1879, 2198, 3181, 3305
HpyF3I CTNAG 8 cut(s) 699, 1125, 1842, 2458, 2583, 2814, 2846, 3024
HpySE526I ACGT 6 cut(s) 1072, 1564, 2119, 2373, 2933, 2938
Hsp92I GRCGYC 1 cut(s) 2373
HspAI GCGC 1 cut(s) 3285
Ksp22I TGATCA 3 cut(s) 28, 1396, 2920
LguI GCTCTTC 1 cut(s) 741
LmnI GCTCC 9 cut(s) 614, 1459, 1660, 1735, 2053, 2497, 3253, 3271, 3292
Lsp1109I GCAGC 2 cut(s) 847, 3317
LweI GCATC 5 cut(s) 39, 502, 1111, 1800, 2827
MaeI CTAG 3 cut(s) 195, 3176, 3216
MaeII ACGT 6 cut(s) 1072, 1564, 2119, 2373, 2933, 2938
MaeIII GTNAC 6 cut(s) 935, 1426, 1482, 1571, 2561, 2934
MbiI CCGCTC 1 cut(s) 3023
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 6 cut(s) 1453, 1665, 2247, 2400, 2783, 3276
MluI ACGCGT 1 cut(s) 1542
MlyI GAGTC 4 cut(s) 566, 640, 751, 1001
MmeI TCCRAC 2 cut(s) 2107, 3089
Mph1103I ATGCAT 1 cut(s) 962
MroXI GAANNNNTTC 3 cut(s) 1623, 2006, 2949
MspA1I CMGCKG 2 cut(s) 1873, 2162
MspCI CTTAAG 1 cut(s) 602
MspI CCGG 4 cut(s) 1717, 2030, 2339, 3338
MspR9I CCNGG 4 cut(s) 1500, 1717, 1718, 3269
Mva1269I GAATGC 2 cut(s) 962, 2720
MvaI CCWGG 2 cut(s) 1500, 3269
MvnI CGCG 1 cut(s) 1544
MwoI GCNNNNNNNGC 6 cut(s) 844, 1870, 1879, 2198, 3181, 3305
NciI CCSGG 2 cut(s) 1717, 1718
NcoI CCATGG 2 cut(s) 93, 1209
NdeI CATATG 5 cut(s) 334, 568, 687, 820, 942
NlaIV GGNNCC 4 cut(s) 913, 2952, 3057, 3224
NmuCI GTSAC 2 cut(s) 935, 1571
NsiI ATGCAT 1 cut(s) 962
NspI RCATGY 2 cut(s) 624, 1552
OliI CACNNNNGTG 2 cut(s) 1211, 1344
PaeR7I CTCGAG 1 cut(s) 2855
PagI TCATGA 2 cut(s) 9, 1774
PciSI GCTCTTC 1 cut(s) 741
PcsI WCGNNNNNNNCGW 2 cut(s) 1918, 2223
PctI GAATGC 2 cut(s) 962, 2720
PdmI GAANNNNTTC 3 cut(s) 1623, 2006, 2949
PfeI GAWTC 5 cut(s) 168, 317, 824, 3028, 3316
PflFI GACNNNGTC 3 cut(s) 799, 1781, 3209
PflMI CCANNNNNTGG 1 cut(s) 94
PkrI GCNGC 2 cut(s) 837, 3307
Ple19I CGATCG 1 cut(s) 2426
PleI GAGTC 4 cut(s) 566, 640, 750, 1000
PpsI GAGTC 4 cut(s) 566, 640, 750, 1000
Ppu21I YACGTR 2 cut(s) 1565, 2939
PshBI ATTAAT 2 cut(s) 963, 1221
PsiI TTATAA 1 cut(s) 521
Psp6I CCWGG 2 cut(s) 1498, 3267
PspEI GGTNACC 1 cut(s) 935
PspFI CCCAGC 2 cut(s) 488, 2568
PspGI CCWGG 2 cut(s) 1498, 3267
PspN4I GGNNCC 4 cut(s) 913, 2952, 3057, 3224
PspPI GGNCC 2 cut(s) 2027, 2975
PspXI VCTCGAGB 1 cut(s) 2855
PstI CTGCAG 1 cut(s) 645
PstNI CAGNNNCTG 1 cut(s) 649
PsuI RGATCY 1 cut(s) 151
PsyI GACNNNGTC 3 cut(s) 799, 1781, 3209
PvuI CGATCG 1 cut(s) 2426
PvuII CAGCTG 1 cut(s) 2162
SapI GCTCTTC 1 cut(s) 741
SatI GCNGC 2 cut(s) 836, 3306
Sau96I GGNCC 2 cut(s) 2027, 2975
ScaI AGTACT 1 cut(s) 2149
SchI GAGTC 4 cut(s) 566, 640, 751, 1001
ScrFI CCNGG 4 cut(s) 1500, 1717, 1718, 3269
SduI GDGCHC 6 cut(s) 1453, 1665, 2247, 2400, 2783, 3276
SfaNI GCATC 5 cut(s) 39, 502, 1111, 1800, 2827
SfcI CTRYAG 1 cut(s) 641
Sfr274I CTCGAG 1 cut(s) 2855
SinI GGWCC 2 cut(s) 2027, 2975
SlaI CTCGAG 1 cut(s) 2855
SmaI CCCGGG 1 cut(s) 1718
SmlI CTYRAG 4 cut(s) 602, 1076, 2300, 2855
SmoI CTYRAG 4 cut(s) 602, 1076, 2300, 2855
SsiI CCGC 7 cut(s) 314, 515, 530, 1487, 1873, 2362, 3021
SspI AATATT 1 cut(s) 1277
SspMI CTAG 3 cut(s) 195, 3176, 3216
StyD4I CCNGG 4 cut(s) 1498, 1715, 1716, 3267
StyI CCWWGG 6 cut(s) 93, 270, 610, 1209, 2736, 3090
TaiI ACGT 6 cut(s) 1075, 1567, 2122, 2376, 2936, 2941
TaqII GACCGA 1 cut(s) 1399
TatI WGTACW 4 cut(s) 762, 1557, 2147, 2658
TfiI GAWTC 5 cut(s) 168, 317, 824, 3028, 3316
TscAI CASTG 9 cut(s) 772, 1333, 1348, 1406, 1458, 1866, 2254, 2788, 3208
TseFI GTSAC 2 cut(s) 935, 1571
TseI GCWGC 2 cut(s) 835, 3305
Tsp45I GTSAC 2 cut(s) 935, 1571
TspMI CCCGGG 1 cut(s) 1716
TspRI CASTG 9 cut(s) 772, 1333, 1348, 1406, 1458, 1866, 2254, 2788, 3208
Tth111I GACNNNGTC 3 cut(s) 799, 1781, 3209
Van91I CCANNNNNTGG 1 cut(s) 94
Vha464I CTTAAG 1 cut(s) 602
VneI GTGCAC 1 cut(s) 2396
VpaK11BI GGWCC 2 cut(s) 2027, 2975
VspI ATTAAT 2 cut(s) 963, 1221
XagI CCTNNNNNAGG 2 cut(s) 1826, 2225
XapI RAATTY 7 cut(s) 325, 1409, 1523, 2276, 3039, 3143, 3345
XceI RCATGY 2 cut(s) 624, 1552
XcmI CCANNNNNNNNNTGG 2 cut(s) 2303, 2743
XhoI CTCGAG 1 cut(s) 2855
XmaI CCCGGG 1 cut(s) 1716
XmiI GTMKAC 2 cut(s) 2607, 3000
XmnI GAANNNNTTC 3 cut(s) 1623, 2006, 2949
XspI CTAG 3 cut(s) 195, 3176, 3216
ZraI GACGTC 1 cut(s) 2374
ZrmI AGTACT 1 cut(s) 2149
Zsp2I ATGCAT 1 cut(s) 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.