Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0000333

Physical Location & Seq

Genomic Coordinates Reverse (-)

23470370 .. 23482147

Gene Structure 11778 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG246600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3192 bp

ATGAAGTTCACTTGGACAATTGAGAGCTTTTCGAGGTTGAACACCAAAAAGCACTACTCTGACATGTTCATCGTCGGTGGTTATAAATGGCGGATACTGATTTTTCCTAAAGGAAACAATGTGGACTACTTGTCAATGTATTTGGATGTGGCGGATTCGCAGGCGCTGCCTTATGGTTGGAGTAGATATGCGCAGTTTAGCTTGGCTATAGTTAATCAGATTCACAACAAGTACTCAATTAGAAAGGACACACAGCACCAATTCAATGCACGAGAAAGTGATTGGGGATTCACATCATTCATGCCTCTCAGTGATCTTTATGACCCCAGCAGGGGTTACCTGGTAAATGATACAGTCGTTGTTGAAGCCGAGGTTGTTGTCCGTAAGGTCCTTGATTACTGGTCGTATGACTCAAAAAAGGAAACTGGTTATGTTGGGCTCAAGAATCAGGGAGCAACTTGTTATATGAATTCACTCCTCCAGACTTTATACCATATACCTTACTTCAGAAAGGCTGTGTATCATATGCCAACAACTGAGAATGACATGCCTTCAGGAAGCATCCCTTTGGCTCTTCAAAGTTTATTCTATAAGCTTCAATACAACGAGAGCAGTGTTGCAACTAAAGAATTAACCAAGTCTTTTGGATGGGATACATATGATTCGTTTATGCAACATGATGTGCAGGAACTTAATAGAGTTCTATGTGAAAAGCTTGAGGATAAAATGAAGGGAACTGTGGTGGAGGGTACAATACAGCAGTTATTTGAAGGACATCACATGAATTACATTGAATGCATCAATGTGGACTACAAATCTACAAGAAAAGAGTCATTTTATGATCTCCAGCTTGATGTAAAAGGATGTCGTGATGTTTACGCTTCTTTTGACAAGTATGTTGAAGTTGAACGTCTTGAGGGTGACAATAAGTACCATGCAGAAGAACATGGTTTGCAGGATGCTAAGAAGGGTGTCCTGTTTATTGACTTCCCTCCAGTTCTTCAGCTTCAGCTAAAGCGATTTGAGTATGATTTTATGAGGGACACTATGGTAAAGATAAATGATCGCTATGAATTTCCTCTTCAACTTGACCTTGATAGGGAGAATGGCAAATATCTGTCACCTGAATCAGATAAGAGTGTGCGGAACCTCTACACTCTTCATAGTGTCTTAGTTCATAGTGGTGGCGTGCATGGAGGACATTATTATGCTTTTATCAGGCCAACCCTTACTGATCAGTGGTATAAGTTTGATGATGAACGCGTGACAAAAGAGGATATGAAGAGGGCATTAGAGGAACAGTATGGTGGTGAGGAAGAGATGCCCCAGACCAATCCTGGTTTTAATAATACTCCTTTCAAGTTTACAAAATACTCGAACGCATACATGCTTGTGTACATCCGGGACAGTGACAAGGACAAAATAATATGTGATGTGGATGAGAAGGACATAGCTGAACACCTAAGGATAAGGTTGAAGAAAGAACAAGAGGAGAAGGAGGACAAGAGGAGATATAAAGCACAGGCCCATCTTTATACTATTATTAAGGTTGCTCGAGATGAGGACCTAGCAGAACAAATTGGAAGGGACATATATTTTGACCTTGTGGACCATGACAAAGTTCGTAGTTTTCGAATCCAGAAACAGACGCTCTTTAACCTTTTCAAGGAGGAAGTTGCGAAAGAGTTGGGGATACCAGTGCAATTTCAGCGATTCTGGATTTGGGCCAAGAGGCAAAACCACACATATCGTCCCAACCGACCATTGACACCCCAGGAAGAATTACAAACCGTTGGACAATTGAGGGAGGCATCAAATAAAACACACAATGCAGAGCTCAAGTTGTTTTTGGAAGTAGAGCTTGGGCCGGAGAAAATTCCTATTCCTCCACCAGACAAAACCAAGGATGATATTCTGCTTTTCTTTAAGCTTTATGAACCTGAGAAACGAGAATTACGGTTTGTTGGTCGGCTTTTTGTTAAGAGTTCTACCAAGCCAATAGAAATAATAGCAAAAATTAATCAAATGGCTGGTTTTGCTCCTGATGAAGAAATTGAACTCTATGAGGAAATAAAGTTTGAGCCTTGTATCATGTGTGAACATCTTGACAAGAGGACCTCATTTCGATTAAGTCAGATTGAAGATGGGGACATTATATGCTTTCAAAAACCTACTCCACTTGAAAGTGAAGAATGTAAATATCCTGACGTCCCTTCATTTTTGGAATATGTACACAACCGTCAGATTGTTCATTTTCGCTCTTTGGAGAAACCAAAAGAGGATGATTTTTCTTTAGAATTGTCAAAGCTACACACTTATGATGATGTGGTGGAGAAGGTGGCTTCCCAAATTGGTTTGGATGATCCTACCAAAATCAGACTCACTGCGCATAACTGCTATTCTCAACAACCTAAACCCCAACCCATCAAATATCGAGGAGTAGAGCATTTAACAGATATGCTGGTCCACTATAATCAAAGTTCTGATATTTTGTATTATGAAGTCTTGGACATCCCCCTGCCAGAATTGCAAGGCCTAAAAAATCTGAAAGTTGCATTTCATCATGCTACAAAAGATGAGGTGGTGATTCACAATATAAGATTGCCCAAGCAGAGCACTGTTGGGGATGTGATTAATGTGCTAAAAACGAAGGTAGAGCTATCTCAACCAAATGCAGAACTCCGACTTCTTGAGGTTTTCTATCACAAGATCTACAAGATCTTCCCACACAGTGAAAAAATTGAGAATATAAATGACCAGTACTGGACATTGCGTGCAGAGGAGATTCCAGAAGAAGAGAAAAACTTGGGTCTTCATGACCGCTTGATTCATGTTTACCACTTTACAAAAGAGACTGCACAGAACCAGATGCAGGTACAGAATTTTGGGGAACCTTTCTTCTTGGTTATTCATGAAGGTGAAACTTTAGCTGAAGTGAAGGCACGCATACAAAAGAAATTGCAGGTCCCTGATGAAGAGTTCGCAAAGTGGAAGTTTGCATTCTTGTCATTGGGTCGTCCCGAGTACCTGCAGGACTCCGATATAGTTTCCAGTCGTTTTCAGAGGAGAGATGTTTATGGTGCTTGGGAGCAGTACCTGGGGTTGGAGCATGCTGATACTGCTCCTAAGAGATCATATGCAGTAAATCAGAACCGTCACACATATGAGAAGCCAGTTAAAATATACAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004843 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009867 GO:0009987 GO:0010033 GO:0016787 GO:0019538 GO:0019783 GO:0023052 GO:0032870 GO:0036459 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070011 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0071704 GO:0101005 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04068 ko05168 ko05169 ko05203 map04068 map05168 map05169 map05203

Pfam Domains

Protein Families

MATH UCH USP7_C2 USP7_ICP0_bdg

Protein Analysis

1063

Amino Acids

125.31

Weight (kDa)

5.88

Isoelectric Point (pI)

44.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MATH_2	PF22486	2 - 125	1.8e-39	MATH domain
MATH	PF00917	7 - 127	2.8e-18	MATH domain
UCH	PF00443	145 - 466	8.4e-60	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	145 - 420	4.5e-15	Ubiquitin carboxyl-terminal hydrolase
USP7_ICP0_bdg	PF12436	570 - 821	2.9e-78	ICP0-binding domain of Ubiquitin-specific protease 7
USP7_C2	PF14533	831 - 1041	3.3e-57	Ubiquitin-specific protease C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11910 AT3G11910 AT3G11910 AT3G11910 AT5G06600 AT5G06600 AT5G06600
fragaria_vesca	FvH4_3g26930 FvH4_3g39132 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220
malus_domestica	MD02G1261100.v1.1 MD03G1161100.v1.1 MD07G1059900.v1.1 MD11G1177100.v1.1 MD12G1047900.v1.1 MD12G1048000.v1.1 MD12G1048400.v1.1 MD12G1048500.v1.1 MD12G1048700.v1.1 MD12G1048800.v1.1 MD12G1056200.v1.1 MD14G1046300.v1.1 MD14G1046500.v1.1 MD15G1166900.v1.1
prunus_persica	Prupe.2G066200_v2.0.a1 Prupe.2G066200_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1
pyrus_communis	pycom02g22320 pycom03g11340 pycom07g04580 pycom11g14290 pycom11g15110 pycom12g04110 pycom12g04140 pycom12g04160 pycom12g04210 pycom12g04220 pycom12g04230 pycom12g04290 pycom12g04320 pycom12g04330 pycom12g04350 pycom12g04360 pycom14g03880 pycom14g03900 pycom16g18750
rosa_chinensis	RchiOBHm_Chr1g0324111 RchiOBHm_Chr1g0324241 RchiOBHm_Chr3g0477691 RchiOBHm_Chr5g0049371 RchiOBHm_Chr5g0070531
rosa_laevigata	RLG00000008612 RLG00000023698 RLG00000034613 RLG00000036139
rosa_multiflora	Rmu_co8271779.1_g000001 Rmu_sc0006187.1_g000014
rosa_roxburghii	Rroxscaffold_1G00010670 Rroxscaffold_1G00030450 Rroxscaffold_1G00031950 Rroxscaffold_1G00031970 Rroxscaffold_1G00032320 Rroxscaffold_1G00055490 Rroxscaffold_2G00083610 Rroxscaffold_2G00084480 Rroxscaffold_2G00115610 Rroxscaffold_3G00261320 Rroxscaffold_4G00312220 Rroxscaffold_4G00325820 Rroxscaffold_5G00339170 Rroxscaffold_5G00364030 Rroxscaffold_6G00403750
rosa_rugosa	Rorug03G0162700 Rorug03G0162800 Rorug03G0162900 Rorug05G0247700 Rorug05G0406600 Rorug05G0406800
rosa_samantha	Rh1AG060300 Rh3AG213000 Rh3BG246600 Rh3CG240400 Rh3DG239700 Rh5AG324400 Rh5AG462100 Rh5BG335500 Rh5BG480200 Rh5BG480300 Rh5CG360200 Rh5CG504500 Rh5CG504600 Rh5DG347800 Rh5DG492200 Rh5DG492300
rosa_wichuraiana	Rw3G019270 Rw3G026910 Rw5G030650 Rw5G032610 Rw5G043040

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	84
AatII	GACGTC	1 cut(s)	2211
Acc16I	TGCGCA	2 cut(s)	192, 2388
Acc36I	ACCTGC	3 cut(s)	2863, 2953, 3036
AccII	CGCG	1 cut(s)	1263
AciI	CCGC	4 cut(s)	91, 152, 1144, 2821
AclWI	GGATC	1 cut(s)	2357
AcsI	RAATTY	4 cut(s)	469, 1073, 1875, 2881
AcuI	CTGAAG	5 cut(s)	490, 537, 986, 992, 2952
AcyI	GRCGYC	1 cut(s)	2208
AdeI	CACNNNGTG	1 cut(s)	2732
AfaI	GTAC	9 cut(s)	233, 751, 932, 1397, 2232, 2762, 2877, 3026, 3095
AfiI	CCNNNNNNNGG	6 cut(s)	331, 332, 1099, 1666, 2872, 3103
AflIII	ACRYGT	2 cut(s)	63, 1261
AhdI	GACNNNNNGTC	1 cut(s)	130
AjnI	CCWGG	4 cut(s)	339, 1336, 1773, 3096
AjuI	GAANNNNNNNTTGG	2 cut(s)	1845, 1877
Alw21I	GWGCWC	2 cut(s)	1839, 2618
Alw26I	GTCTC	1 cut(s)	2846
AlwI	GGATC	1 cut(s)	2357
AlwNI	CAGNNNCTG	2 cut(s)	166, 3097
Ama87I	CYCGRG	2 cut(s)	1554, 3020
AoxI	GGCC	5 cut(s)	1220, 1524, 1725, 1865, 2533
ApeKI	GCWGC	1 cut(s)	166
ApoI	RAATTY	4 cut(s)	469, 1073, 1875, 2881
ArsI	GACNNNNNNTTYG	2 cut(s)	1580, 1612
AseI	ATTAAT	2 cut(s)	2019, 2634
AspLEI	GCGC	3 cut(s)	166, 193, 2389
AspS9I	GGNCC	9 cut(s)	388, 1525, 1564, 1609, 1725, 1865, 2115, 2464, 2965
AsuC2I	CCSGG	1 cut(s)	1403
AsuHPI	GGTGA	5 cut(s)	932, 1113, 1322, 2596, 2930
AsuII	TTCGAA	1 cut(s)	1633
AvaI	CYCGRG	2 cut(s)	1554, 3020
AvaII	GGWCC	6 cut(s)	388, 1564, 1609, 2115, 2464, 2965
AxyI	CCTNAGG	1 cut(s)	1463
BaeI	ACNNNNGTAYC	2 cut(s)	342, 375
BanII	GRGCYC	2 cut(s)	441, 1839
BauI	CACGAG	1 cut(s)	270
BbsI	GAAGAC	1 cut(s)	2804
Bbv12I	GWGCWC	2 cut(s)	1839, 2618
BbvI	GCAGC	1 cut(s)	153
BccI	CCATC	4 cut(s)	642, 1536, 2138, 2432
BciT130I	CCWGG	4 cut(s)	341, 1338, 1775, 3098
BciVI	GTATCC	3 cut(s)	87, 646, 1686
BclI	TGATCA	1 cut(s)	1234
BcnI	CCSGG	1 cut(s)	1403
BcoDI	GTCTC	1 cut(s)	2846
BfaI	CTAG	1 cut(s)	1568
BfmI	CTRYAG	2 cut(s)	207, 3029
BfoI	RGCGCY	1 cut(s)	167
BfuAI	ACCTGC	3 cut(s)	2863, 2953, 3036
BfuI	GTATCC	3 cut(s)	87, 646, 1686
BglII	AGATCT	2 cut(s)	2709, 2718
BisI	GCNGC	1 cut(s)	167
BlsI	GCNGC	1 cut(s)	168
BmcAI	AGTACT	2 cut(s)	233, 2762
Bme1390I	CCNGG	5 cut(s)	341, 1338, 1403, 1775, 3098
Bme18I	GGWCC	6 cut(s)	388, 1564, 1609, 2115, 2464, 2965
BmeRI	GACNNNNNGTC	1 cut(s)	130
BmeT110I	CYCGRG	2 cut(s)	1554, 3020
BmgT120I	GGNCC	9 cut(s)	388, 1525, 1564, 1609, 1725, 1865, 2115, 2464, 2965
BmiI	GGNNCC	3 cut(s)	1148, 2892, 2967
BmrFI	CCNGG	5 cut(s)	341, 1338, 1403, 1775, 3098
BmsI	GCATC	6 cut(s)	570, 807, 949, 1311, 1820, 2859
BpiI	GAAGAC	1 cut(s)	2804
BpmI	CTGGAG	3 cut(s)	464, 830, 978
Bpu14I	TTCGAA	1 cut(s)	1633
BpuEI	CTTGAG	5 cut(s)	425, 737, 935, 1823, 2711
BpuMI	CCSGG	1 cut(s)	1403
BsaBI	GATNNNNATC	1 cut(s)	292
BsaHI	GRCGYC	1 cut(s)	2208
BsaJI	CCNNGG	4 cut(s)	369, 1773, 1902, 3097
Bsc4I	CCNNNNNNNGG	6 cut(s)	331, 332, 1099, 1666, 2872, 3103
Bse1I	ACTGG	8 cut(s)	404, 430, 995, 1697, 2758, 2768, 3051, 3173
Bse21I	CCTNAGG	1 cut(s)	1463
Bse3DI	GCAATG	1 cut(s)	2768
Bse8I	GATNNNNATC	1 cut(s)	292
BseBI	CCWGG	4 cut(s)	341, 1338, 1775, 3098
BseDI	CCNNGG	4 cut(s)	369, 1773, 1902, 3097
BseJI	GATNNNNATC	1 cut(s)	292
BseLI	CCNNNNNNNGG	6 cut(s)	331, 332, 1099, 1666, 2872, 3103
BseMI	GCAATG	1 cut(s)	2768
BseMII	CTCAG	3 cut(s)	322, 528, 1932
BseNI	ACTGG	8 cut(s)	404, 430, 995, 1697, 2758, 2768, 3051, 3173
BseRI	GAGGAG	6 cut(s)	467, 1505, 1522, 2451, 2795, 3079
BseXI	GCAGC	1 cut(s)	153
BseYI	CCCAGC	1 cut(s)	326
BsgI	GTGCAG	3 cut(s)	704, 2796, 2841
Bsh1236I	CGCG	1 cut(s)	1263
BshFI	GGCC	5 cut(s)	1222, 1526, 1727, 1867, 2535
BsiHKAI	GWGCWC	2 cut(s)	1839, 2618
BsiHKCI	CYCGRG	2 cut(s)	1554, 3020
BsiSI	CCGG	2 cut(s)	1402, 1868
BslFI	GGGAC	8 cut(s)	1055, 1418, 1601, 1737, 2162, 2195, 2951, 3003
BslI	CCNNNNNNNGG	6 cut(s)	331, 332, 1099, 1666, 2872, 3103
BsmAI	GTCTC	1 cut(s)	2846
BsmFI	GGGAC	8 cut(s)	1055, 1418, 1601, 1737, 2162, 2195, 2951, 3003
BsmI	GAATGC	2 cut(s)	800, 2999
BsnI	GGCC	5 cut(s)	1222, 1526, 1727, 1867, 2535
BsoBI	CYCGRG	2 cut(s)	1554, 3020
Bsp119I	TTCGAA	1 cut(s)	1633
Bsp1286I	GDGCHC	3 cut(s)	441, 1839, 2618
Bsp1407I	TGTACA	2 cut(s)	1395, 2230
Bsp143I	GATC	8 cut(s)	313, 841, 1063, 1234, 2362, 2709, 2718, 3131
BspACI	CCGC	4 cut(s)	91, 152, 1144, 2821
BspANI	GGCC	5 cut(s)	1222, 1526, 1727, 1867, 2535
BspCNI	CTCAG	3 cut(s)	321, 529, 1933
BspFNI	CGCG	1 cut(s)	1263
BspHI	TCATGA	2 cut(s)	2815, 2911
BspLI	GGNNCC	3 cut(s)	1148, 2892, 2967
BspMAI	CTGCAG	1 cut(s)	3033
BspMI	ACCTGC	3 cut(s)	2863, 2953, 3036
BspPI	GGATC	1 cut(s)	2357
BspQI	GCTCTTC	1 cut(s)	579
BspT104I	TTCGAA	1 cut(s)	1633
BsrDI	GCAATG	1 cut(s)	2768
BsrGI	TGTACA	2 cut(s)	1395, 2230
BsrI	ACTGG	8 cut(s)	404, 430, 995, 1697, 2758, 2768, 3051, 3173
BssECI	CCNNGG	4 cut(s)	369, 1773, 1902, 3097
BssMI	GATC	8 cut(s)	313, 841, 1063, 1234, 2362, 2709, 2718, 3131
BssNI	GRCGYC	1 cut(s)	2208
BssSI	CACGAG	1 cut(s)	270
BssT1I	CCWWGG	1 cut(s)	1902
Bst2BI	CACGAG	1 cut(s)	270
Bst2UI	CCWGG	4 cut(s)	341, 1338, 1775, 3098
Bst6I	CTCTTC	7 cut(s)	579, 1086, 1164, 1277, 1311, 2790, 2970
BstACI	GRCGYC	1 cut(s)	2208
BstAPI	GCANNNNNTGC	1 cut(s)	166
BstAUI	TGTACA	2 cut(s)	1395, 2230
BstBI	TTCGAA	1 cut(s)	1633
BstC8I	GCNNGC	5 cut(s)	162, 1190, 2775, 2944, 3111
BstDEI	CTNAG	7 cut(s)	308, 537, 963, 1171, 1463, 1941, 3126
BstEII	GGTNACC	1 cut(s)	335
BstENI	CCTNNNNNAGG	1 cut(s)	1664
BstFNI	CGCG	1 cut(s)	1263
BstH2I	RGCGCY	1 cut(s)	167
BstHHI	GCGC	3 cut(s)	166, 193, 2389
BstKTI	GATC	8 cut(s)	316, 844, 1066, 1237, 2365, 2712, 2721, 3134
BstMAI	GTCTC	1 cut(s)	2846
BstMBI	GATC	8 cut(s)	313, 841, 1063, 1234, 2362, 2709, 2718, 3131
BstMWI	GCNNNNNNNGC	5 cut(s)	166, 1708, 2036, 2527, 3119
BstNI	CCWGG	4 cut(s)	341, 1338, 1775, 3098
BstNSI	RCATGY	4 cut(s)	67, 550, 1390, 3113
BstPI	GGTNACC	1 cut(s)	335
BstSCI	CCNGG	5 cut(s)	339, 1336, 1401, 1773, 3096
BstSFI	CTRYAG	2 cut(s)	207, 3029
BstUI	CGCG	1 cut(s)	1263
BstV1I	GCAGC	1 cut(s)	153
BstV2I	GAAGAC	1 cut(s)	2804
BstX2I	RGATCY	2 cut(s)	2709, 2718
BstYI	RGATCY	2 cut(s)	2709, 2718
Bsu36I	CCTNAGG	1 cut(s)	1463
BsuI	GTATCC	3 cut(s)	87, 646, 1686
BsuRI	GGCC	5 cut(s)	1222, 1526, 1727, 1867, 2535
BtsI	GCAGTG	2 cut(s)	619, 2382
BtsIMutI	CAGTG	8 cut(s)	316, 619, 1244, 1414, 1704, 2382, 2616, 2737
BveI	ACCTGC	3 cut(s)	2863, 2953, 3036
Cac8I	GCNNGC	5 cut(s)	162, 1190, 2775, 2944, 3111
CaiI	CAGNNNCTG	2 cut(s)	166, 3097
CciI	TCATGA	2 cut(s)	2815, 2911
CfoI	GCGC	3 cut(s)	166, 193, 2389
Cfr13I	GGNCC	9 cut(s)	388, 1525, 1564, 1609, 1725, 1865, 2115, 2464, 2965
CseI	GACGC	1 cut(s)	1657
CsiI	ACCWGGT	1 cut(s)	339
Csp6I	GTAC	9 cut(s)	232, 750, 931, 1396, 2231, 2761, 2876, 3025, 3094
CviQI	GTAC	9 cut(s)	232, 750, 931, 1396, 2231, 2761, 2876, 3025, 3094
DdeI	CTNAG	7 cut(s)	308, 537, 963, 1171, 1463, 1941, 3126
DpnI	GATC	8 cut(s)	315, 843, 1065, 1236, 2364, 2711, 2720, 3133
DpnII	GATC	8 cut(s)	313, 841, 1063, 1234, 2362, 2709, 2718, 3131
DraIII	CACNNNGTG	1 cut(s)	2732
DriI	GACNNNNNGTC	1 cut(s)	130
Eam1104I	CTCTTC	7 cut(s)	579, 1086, 1164, 1277, 1311, 2790, 2970
Eam1105I	GACNNNNNGTC	1 cut(s)	130
EarI	CTCTTC	7 cut(s)	579, 1086, 1164, 1277, 1311, 2790, 2970
EciI	GGCGGA	2 cut(s)	106, 167
Ecl136II	GAGCTC	1 cut(s)	1837
Eco130I	CCWWGG	1 cut(s)	1902
Eco147I	AGGCCT	1 cut(s)	2535
Eco24I	GRGCYC	2 cut(s)	441, 1839
Eco47I	GGWCC	6 cut(s)	388, 1564, 1609, 2115, 2464, 2965
Eco53kI	GAGCTC	1 cut(s)	1837
Eco57I	CTGAAG	5 cut(s)	490, 537, 986, 992, 2952
Eco81I	CCTNAGG	1 cut(s)	1463
Eco88I	CYCGRG	2 cut(s)	1554, 3020
Eco91I	GGTNACC	1 cut(s)	335
EcoICRI	GAGCTC	1 cut(s)	1837
EcoNI	CCTNNNNNAGG	1 cut(s)	1664
EcoO109I	RGGNCCY	4 cut(s)	388, 1564, 2115, 2965
EcoO65I	GGTNACC	1 cut(s)	335
EcoRI	GAATTC	1 cut(s)	469
EcoRII	CCWGG	4 cut(s)	339, 1336, 1773, 3096
EcoT14I	CCWWGG	1 cut(s)	1902
EcoT22I	ATGCAT	1 cut(s)	800
EcoT38I	GRGCYC	2 cut(s)	441, 1839
ErhI	CCWWGG	1 cut(s)	1902
FalI	AAGNNNNNCTT	4 cut(s)	550, 582, 1845, 1877
FaqI	GGGAC	8 cut(s)	1055, 1418, 1601, 1737, 2162, 2195, 2951, 3003
FauNDI	CATATG	4 cut(s)	525, 658, 3136, 3163
FbaI	TGATCA	1 cut(s)	1234
Fnu4HI	GCNGC	1 cut(s)	167
FriOI	GRGCYC	2 cut(s)	441, 1839
Fsp4HI	GCNGC	1 cut(s)	167
FspBI	CTAG	1 cut(s)	1568
FspI	TGCGCA	2 cut(s)	192, 2388
GlaI	GCGC	3 cut(s)	165, 192, 2388
GluI	GCNGC	1 cut(s)	167
GsaI	CCCAGC	1 cut(s)	330
GsuI	CTGGAG	3 cut(s)	464, 830, 978
HaeII	RGCGCY	1 cut(s)	167
HaeIII	GGCC	5 cut(s)	1222, 1526, 1727, 1867, 2535
HapII	CCGG	2 cut(s)	1402, 1868
HgaI	GACGC	1 cut(s)	1657
HhaI	GCGC	3 cut(s)	166, 193, 2389
Hin1I	GRCGYC	1 cut(s)	2208
Hin6I	GCGC	3 cut(s)	164, 191, 2387
HinP1I	GCGC	3 cut(s)	164, 191, 2387
HindIII	AAGCTT	3 cut(s)	593, 713, 1928
HpaII	CCGG	2 cut(s)	1402, 1868
HphI	GGTGA	5 cut(s)	932, 1113, 1322, 2596, 2930
Hpy99I	CGWCG	1 cut(s)	77
HpyCH4IV	ACGT	2 cut(s)	910, 2208
HpyF10VI	GCNNNNNNNGC	5 cut(s)	166, 1708, 2036, 2527, 3119
HpyF3I	CTNAG	7 cut(s)	308, 537, 963, 1171, 1463, 1941, 3126
HpySE526I	ACGT	2 cut(s)	910, 2208
Hsp92I	GRCGYC	1 cut(s)	2208
HspAI	GCGC	3 cut(s)	164, 191, 2387
Ksp22I	TGATCA	1 cut(s)	1234
Kzo9I	GATC	8 cut(s)	313, 841, 1063, 1234, 2362, 2709, 2718, 3131
LguI	GCTCTTC	1 cut(s)	579
LmnI	GCTCC	5 cut(s)	452, 2044, 3088, 3106, 3127
Lsp1109I	GCAGC	1 cut(s)	153
LweI	GCATC	6 cut(s)	570, 807, 949, 1311, 1820, 2859
MabI	ACCWGGT	1 cut(s)	339
MaeI	CTAG	1 cut(s)	1568
MaeII	ACGT	2 cut(s)	910, 2208
MaeIII	GTNAC	6 cut(s)	335, 920, 1119, 1264, 1409, 3155
MalI	GATC	8 cut(s)	315, 843, 1065, 1236, 2364, 2711, 2720, 3133
MboI	GATC	8 cut(s)	313, 841, 1063, 1234, 2362, 2709, 2718, 3131
MfeI	CAATTG	2 cut(s)	18, 1799
MflI	RGATCY	2 cut(s)	2709, 2718
MhlI	GDGCHC	3 cut(s)	441, 1839, 2618
MluI	ACGCGT	1 cut(s)	1261
MlyI	GAGTC	4 cut(s)	404, 839, 2373, 3029
MmeI	TCCRAC	4 cut(s)	158, 1774, 2707, 3084
Mph1103I	ATGCAT	1 cut(s)	800
MslI	CAYNNNNRTG	7 cut(s)	803, 1182, 1206, 1391, 2316, 2916, 3162
MspI	CCGG	2 cut(s)	1402, 1868
MspR9I	CCNGG	5 cut(s)	341, 1338, 1403, 1775, 3098
MunI	CAATTG	2 cut(s)	18, 1799
Mva1269I	GAATGC	2 cut(s)	800, 2999
MvaI	CCWGG	4 cut(s)	341, 1338, 1775, 3098
MvnI	CGCG	1 cut(s)	1263
MwoI	GCNNNNNNNGC	5 cut(s)	166, 1708, 2036, 2527, 3119
NciI	CCSGG	1 cut(s)	1403
NdeI	CATATG	4 cut(s)	525, 658, 3136, 3163
NdeII	GATC	8 cut(s)	313, 841, 1063, 1234, 2362, 2709, 2718, 3131
NlaIV	GGNNCC	3 cut(s)	1148, 2892, 2967
NmeAIII	GCCGAG	1 cut(s)	394
NmuCI	GTSAC	5 cut(s)	920, 1119, 1264, 1409, 3155
NsbI	TGCGCA	2 cut(s)	192, 2388
NsiI	ATGCAT	1 cut(s)	800
NspI	RCATGY	4 cut(s)	67, 550, 1390, 3113
NspV	TTCGAA	1 cut(s)	1633
PaeI	GCATGC	1 cut(s)	3113
PaeR7I	CTCGAG	1 cut(s)	1554
PagI	TCATGA	2 cut(s)	2815, 2911
PceI	AGGCCT	1 cut(s)	2535
PciI	ACATGT	1 cut(s)	63
PciSI	GCTCTTC	1 cut(s)	579
PcsI	WCGNNNNNNNCGW	2 cut(s)	1630, 1756
PctI	GAATGC	2 cut(s)	800, 2999
PfoI	TCCNGGA	1 cut(s)	1401
PkrI	GCNGC	1 cut(s)	168
PleI	GAGTC	4 cut(s)	404, 838, 2373, 3029
PpsI	GAGTC	4 cut(s)	404, 838, 2373, 3029
PpuMI	RGGWCCY	4 cut(s)	388, 1564, 2115, 2965
PscI	ACATGT	1 cut(s)	63
PshBI	ATTAAT	2 cut(s)	2019, 2634
PsiI	TTATAA	1 cut(s)	84
Psp124BI	GAGCTC	1 cut(s)	1839
Psp5II	RGGWCCY	4 cut(s)	388, 1564, 2115, 2965
Psp6I	CCWGG	4 cut(s)	339, 1336, 1773, 3096
PspEI	GGTNACC	1 cut(s)	335
PspFI	CCCAGC	1 cut(s)	326
PspGI	CCWGG	4 cut(s)	339, 1336, 1773, 3096
PspN4I	GGNNCC	3 cut(s)	1148, 2892, 2967
PspPI	GGNCC	9 cut(s)	388, 1525, 1564, 1609, 1725, 1865, 2115, 2464, 2965
PspPPI	RGGWCCY	4 cut(s)	388, 1564, 2115, 2965
PstI	CTGCAG	1 cut(s)	3033
PstNI	CAGNNNCTG	2 cut(s)	166, 3097
PsuI	RGATCY	2 cut(s)	2709, 2718
RsaI	GTAC	9 cut(s)	233, 751, 932, 1397, 2232, 2762, 2877, 3026, 3095
RsaNI	GTAC	9 cut(s)	232, 750, 931, 1396, 2231, 2761, 2876, 3025, 3094
RseI	CAYNNNNRTG	7 cut(s)	803, 1182, 1206, 1391, 2316, 2916, 3162
SacI	GAGCTC	1 cut(s)	1839
SapI	GCTCTTC	1 cut(s)	579
SatI	GCNGC	1 cut(s)	167
Sau3AI	GATC	8 cut(s)	313, 841, 1063, 1234, 2362, 2709, 2718, 3131
Sau96I	GGNCC	9 cut(s)	388, 1525, 1564, 1609, 1725, 1865, 2115, 2464, 2965
SbfI	CCTGCAGG	1 cut(s)	3033
ScaI	AGTACT	2 cut(s)	233, 2762
SchI	GAGTC	4 cut(s)	404, 839, 2373, 3029
ScrFI	CCNGG	5 cut(s)	341, 1338, 1403, 1775, 3098
SdaI	CCTGCAGG	1 cut(s)	3033
SduI	GDGCHC	3 cut(s)	441, 1839, 2618
SexAI	ACCWGGT	1 cut(s)	339
SfaNI	GCATC	6 cut(s)	570, 807, 949, 1311, 1820, 2859
SfcI	CTRYAG	2 cut(s)	207, 3029
Sfr274I	CTCGAG	1 cut(s)	1554
SfuI	TTCGAA	1 cut(s)	1633
SinI	GGWCC	6 cut(s)	388, 1564, 1609, 2115, 2464, 2965
SlaI	CTCGAG	1 cut(s)	1554
SmiMI	CAYNNNNRTG	7 cut(s)	803, 1182, 1206, 1391, 2316, 2916, 3162
SmlI	CTYRAG	6 cut(s)	440, 716, 914, 1554, 1838, 2690
SmoI	CTYRAG	6 cut(s)	440, 716, 914, 1554, 1838, 2690
SphI	GCATGC	1 cut(s)	3113
Sse8387I	CCTGCAGG	1 cut(s)	3033
SseBI	AGGCCT	1 cut(s)	2535
SsiI	CCGC	4 cut(s)	91, 152, 1144, 2821
SspMI	CTAG	1 cut(s)	1568
SstI	GAGCTC	1 cut(s)	1839
StuI	AGGCCT	1 cut(s)	2535
StyD4I	CCNGG	5 cut(s)	339, 1336, 1401, 1773, 3096
StyI	CCWWGG	1 cut(s)	1902
TaiI	ACGT	2 cut(s)	913, 2211
TaqI	TCGA	6 cut(s)	32, 1376, 1555, 1633, 2125, 2434
TatI	WGTACW	4 cut(s)	231, 1395, 2230, 2760
TscAI	CASTG	8 cut(s)	316, 619, 1244, 1414, 1704, 2389, 2623, 2737
TseFI	GTSAC	5 cut(s)	920, 1119, 1264, 1409, 3155
TseI	GCWGC	1 cut(s)	166
Tsp45I	GTSAC	5 cut(s)	920, 1119, 1264, 1409, 3155
TspGWI	ACGGA	1 cut(s)	371
TspRI	CASTG	8 cut(s)	316, 619, 1244, 1414, 1704, 2389, 2623, 2737
VpaK11BI	GGWCC	6 cut(s)	388, 1564, 1609, 2115, 2464, 2965
VspI	ATTAAT	2 cut(s)	2019, 2634
XagI	CCTNNNNNAGG	1 cut(s)	1664
XapI	RAATTY	4 cut(s)	469, 1073, 1875, 2881
XceI	RCATGY	4 cut(s)	67, 550, 1390, 3113
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1334
XhoI	CTCGAG	1 cut(s)	1554
XspI	CTAG	1 cut(s)	1568
ZraI	GACGTC	1 cut(s)	2209
ZrmI	AGTACT	2 cut(s)	233, 2762
Zsp2I	ATGCAT	1 cut(s)	800

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.