Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0000333

Physical Location & Seq

Genomic Coordinates Reverse (-)

5572428 .. 5584093

Gene Structure 11666 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1048800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3732 bp

ATGCATGTGCCGCTTTCGAATTTGGCTGGAAGCTCTCGGGGCATGGAAGCTCAAGTGGAGCAGACTAATGCATTTGAGGATCCTCAAAAGATGAAATTCACTTGGACAATTGAGAACTTTACTAGGTTGGACAACAAGAACCACTACTCTGACATCTTCGTTGTTGGCGGCTTTAAATGGCGGATAATAATGTTTCCCAAGGGAAACCAAGTGGACGCGTTATCAATGTATTTGGATGCAGCAGATTCTGGGACATTGCCATATGGGTGGAGAAGATTTGCGCACTTTAGCTTGGCTGTAGTTAATCAGATCGATACCAAGTACTCAGTAGAAAAGGACACACAACACCAGTTCAATGCAAGAGAAAGTGACTGGGGATTCACTTCATTCATGCCTCTTGGTGATCTTTATGACCCAAGTAGGGGATATCTGGTGAGCGATACAGTTGTGGTTGAAGCGAAAGTTACTGTCCGTAAGGTTCTTAACTACCAGTCATATGACTCAAAAAAGGAAACTGGTTATGTTGGGCTTGAGAATCAGCGGCAAAGTGGTTATATGAATTCTCTACTCCAGACGTTTTACCATATACCATACTTTAGAAAGGCTGTTCATCATATGCCAACAACTGAGAATGACATGCCTTCAGGAAGCATCCCTTTGACACTAAAACGTTTATTCTATAAGCTTCAATACAGTGACAGCATTGTTTCAACTAAAGAATTGACCAAGTCTTTTGGGTGGGATACACATTATTTGTTTATGAAACATGACGTCCATGAATTTAATAAATTTCTTAGTCAAAAGCTTGAAGATAAAATGAAGGTATGGGAATGTTTCATTTTGACCATGAGTTTAATTTTCATTTCACCTACAATTTTTATAATTGTTATGTTGGTTCAGGGAACTGTTGTGGAGGGTACAATACTGCAGTTATTTGAAGGACATTACATGAACTACACTGACTGCATCAATGTGCACTACAAATCTGAAAGAAAAGAGTCATTTTATGACCTCCAGCTTGATGTCAAAGGCTGTCGGGATGTCTATGATTCTTTCGATAAGTATGTGACAGTTGAACATCTTGAGGGTGACAATAAATACCGTGCGGGACAATATGGTTTGCAGGTTGCTAAGAAGGGTGTTCTGTTTATTGACTTCCCTCCTGTTCTTCAACTTCAGCTAAAGCGATTTGAGTATGATTTTGTGCGGGACACTGTGGTTAAGGTAAATGATCGCTATGAATTTCCTATTCAACTTGACCTTGATAGGGAGAATGGAATGTACCTATCACCTGAATCAGATAGGAGTGTCCGCAACCTCTACACTCTTCATAGTGTCTTGGTTCATATTGGTGGGGCGCATGATGGACGTTACTGTGCTTTCATAAGGCCAACCCTCTCTGACCAGTGGTACAAGTTTGACAATGAACGTGTGACGAAAGAGGATGTGAAAAGGGCTTTAGAAGAGCAGTATGGTGGTGAGAAAGAGTTACCACGGACCAATTCTCACTTTAATCGTACTTCCTTCAGCCTTACAAAATACTCAAATGCATACATGCTTGTGTATATACGGGAAAGTGATAAGGAGAAAATAATGTGTAACGTGGATGAGAAAGACATAGCCGAACATCTAAGGATAAGGTCGAAGAAAGGACAAGAAAAGAAAGAGGTTGAAAGATGTAATGCACAAGCACACCTCTACACTACTATTAAGGTTTCTCGAGATAACGACCTGGCAGAACAAATTGGAAGGGATGTATATTTTGGCCTTGTCGACCATAACAAAGTTCATAGGTTCCGTATTCTGAATCTAACACCCTTTAATCTTTTCAAGAAGGAGGTTGCAAATGAGTTTGGCATACCAGTGCAGTTTCAGCGTTTCTGGATTTGGTCCATGAGAAAAAACCATACATATCGCCCCAAACGACCATTGACAAGTTGTGAAGAATCAAAAGCAGTTGTTTGGTGCCTTGTGCAACTAATGAAGACCACTTGCATTTTCCAGGTTGGACGATTGAGAGATGGATCAAATACAATACACAATGGAGAGCTAAAGTTGTTTTTGGAAGTAGAGTTCGGGCCGGTAATCCAGTGTTACTCTATTCTTGTTATGACCTATGGTGTGAGGGATCTATGTCTTATTCCTCCGCCTGCCAAAACCAAGAAAGATATCCTTGTTTTCTTTAAGCTTTATGAACCTGAGAAACGAGAACTACGTTTTGTTGGTCGGCTTTTTGTGAAGAGTTGTAGTAGGCCAGTAGAGATTTTAGCAAAATTGAATCAGCTTGCTGGTTTTTCCCCTGATGAAGAAATTGAACTCTATCCGGAAATAAGTTCTGAGCCTTGTATCATGTGGGAGCAGCTTGACAAGAAGATCCCATTTCGATTCAGTCAGATTCAAGATGGGGATATAATATGCTTTCAGAAATCTACTCCTCATGGAAGTGAAGTATGTAAATATCCTAACGTTCCTTCATTTTTCAAATACCTACACTATCGTCAGATTGTTCGTTTCCGTTCGCTGGAGAAACCGAATGAGGATGATTTCAGTTTAGAATTGTCAAAGCTTCACACTTATGATGATGTTGTGGAGAATTTGGCTCGCCTAATTGGTTTGGAAAATGCCACCAAAATCAGACTCACTGCACATAACTGCTTATCTCAACAACCTAGGCCCCGACCTATCAAATACCAGGGAGCAGAGCATTTAACAGAAATGTTAACTCATTACAATCATAGCTCTGATATTTTGTACTATGAAGTCTTGGGCATTCCCCTGCCAGAGTTGGAAGGCCTGAAAACTCTAAAAATTGCTTTTCATCATGCTACAAAAGACGAGGTCCGTGAAATATACCGCAACATCTGCTTATGGACGGTGGTGATTCATACTATTAGCTTTCCCAAATGGAAAACTGTTGGGGATTTGATTAATGTACTTCAAACGAAGGTAGAGCTATCTCATCCAAATGTAGAACTCCGAGTGCTTGGCATTTTATATCACAAGATCTACAAGGTTGCTCCATTTGTTAAGCTGATAATGTATCGTATGTATTTGCATATTCCAGAAGAAGAGAAAAAGTTGGGTCAACGTGATCGCTTGATTCATGTTTACCACTTCACAAAAGTCACTGTACTGAAAGACATGGTAGAAGCAAACTATTTCATTGCTGTTTTAGACTTTCAGTCAAAGAAGCATGTACAAACTTTTGGGGAACCTTTTGTCTTGGTCATCCATGAAGGTGAAGGTCTAGCTGAAGTTAAAACACGGATACAAAAGAAATTGCGGGTTCCCGATGAGGAGTTCTCAAAGTGGAACTTTGCATTTTTGTCACGTGGTCATCCAGATTACTTGCAGGATTCCGACATTGTTTTCAGCCGATTTCAGAGAAATGAATCATTGCCTCCATGGGAGCAGTACCTCGGGTTGGAGCACTCTGATAAAGTTCCTAAGAGGGCTTATGCAGTAACTGAAGCAAGTGCTGCTGTACCCAGCAAAAGGAGAAAAGAGGGTCGAAAGGGGGGACAAGTGCCCTATCCCGTTCCAATCTCTACCGATGTCGTTCCAGCGATCACGGGGAGTTCTCAGCCCCAAGGACCTGCCGGGAATGCAGGCAAGGTAGGTGAGATTCCGGCCTTAAATCTATCTTTACCAACCACTGAGCCAGGCAAAGCCAACTCTTCCGGAATGAAAGGAAATGAAGTGTCGGGAAAAACTATTCTTCTCATTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004843 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009867 GO:0009987 GO:0010033 GO:0016787 GO:0019538 GO:0019783 GO:0023052 GO:0032870 GO:0036459 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070011 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0071704 GO:0101005 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04068 ko05168 ko05169 ko05203 map04068 map05168 map05169 map05203

Pfam Domains

Protein Families

MATH UCH USP7_C2 USP7_ICP0_bdg

Protein Analysis

1244

Amino Acids

144.22

Weight (kDa)

8.4

Isoelectric Point (pI)

41.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MATH_2	PF22486	32 - 155	7.4e-33	MATH domain
MATH	PF00917	37 - 157	2.2e-14	MATH domain
UCH	PF00443	175 - 522	6.7e-46	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	175 - 475	1.2e-09	Ubiquitin carboxyl-terminal hydrolase
USP7_ICP0_bdg	PF12436	625 - 907	2.6e-61	ICP0-binding domain of Ubiquitin-specific protease 7
USP7_C2	PF14533	917 - 1148	5e-36	Ubiquitin-specific protease C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11910 AT3G11910 AT3G11910 AT3G11910 AT5G06600 AT5G06600 AT5G06600
fragaria_vesca	FvH4_3g26930 FvH4_3g39132 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220
malus_domestica	MD02G1261100.v1.1 MD03G1161100.v1.1 MD07G1059900.v1.1 MD11G1177100.v1.1 MD12G1047900.v1.1 MD12G1048000.v1.1 MD12G1048400.v1.1 MD12G1048500.v1.1 MD12G1048700.v1.1 MD12G1048800.v1.1 MD12G1056200.v1.1 MD14G1046300.v1.1 MD14G1046500.v1.1 MD15G1166900.v1.1
prunus_persica	Prupe.2G066200_v2.0.a1 Prupe.2G066200_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1
pyrus_communis	pycom02g22320 pycom03g11340 pycom07g04580 pycom11g14290 pycom11g15110 pycom12g04110 pycom12g04140 pycom12g04160 pycom12g04210 pycom12g04220 pycom12g04230 pycom12g04290 pycom12g04320 pycom12g04330 pycom12g04350 pycom12g04360 pycom14g03880 pycom14g03900 pycom16g18750
rosa_chinensis	RchiOBHm_Chr1g0324111 RchiOBHm_Chr1g0324241 RchiOBHm_Chr3g0477691 RchiOBHm_Chr5g0049371 RchiOBHm_Chr5g0070531
rosa_laevigata	RLG00000008612 RLG00000023698 RLG00000034613 RLG00000036139
rosa_multiflora	Rmu_co8271779.1_g000001 Rmu_sc0006187.1_g000014
rosa_roxburghii	Rroxscaffold_1G00010670 Rroxscaffold_1G00030450 Rroxscaffold_1G00031950 Rroxscaffold_1G00031970 Rroxscaffold_1G00032320 Rroxscaffold_1G00055490 Rroxscaffold_2G00083610 Rroxscaffold_2G00084480 Rroxscaffold_2G00115610 Rroxscaffold_3G00261320 Rroxscaffold_4G00312220 Rroxscaffold_4G00325820 Rroxscaffold_5G00339170 Rroxscaffold_5G00364030 Rroxscaffold_6G00403750
rosa_rugosa	Rorug03G0162700 Rorug03G0162800 Rorug03G0162900 Rorug05G0247700 Rorug05G0406600 Rorug05G0406800
rosa_samantha	Rh1AG060300 Rh3AG213000 Rh3BG246600 Rh3CG240400 Rh3DG239700 Rh5AG324400 Rh5AG462100 Rh5BG335500 Rh5BG480200 Rh5BG480300 Rh5CG360200 Rh5CG504500 Rh5CG504600 Rh5DG347800 Rh5DG492200 Rh5DG492300
rosa_wichuraiana	Rw3G019270 Rw3G026910 Rw5G030650 Rw5G032610 Rw5G043040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	881
AatII	GACGTC	1 cut(s)	774
Acc16I	TGCGCA	1 cut(s)	282
Acc36I	ACCTGC	2 cut(s)	1114, 3604
AccB1I	GGYRCC	1 cut(s)	1965
AccI	GTMKAC	1 cut(s)	1773
AccII	CGCG	1 cut(s)	218
AccIII	TCCGGA	2 cut(s)	2323, 3680
AclI	AACGTT	2 cut(s)	670, 2466
AclWI	GGATC	5 cut(s)	74, 87, 2032, 2136, 2368
AcsI	RAATTY	7 cut(s)	19, 95, 559, 779, 788, 1241, 2593
AcuI	CTGAAG	5 cut(s)	627, 1160, 1510, 3273, 3489
AcvI	CACGTG	1 cut(s)	3332
AcyI	GRCGYC	1 cut(s)	771
AfiI	CCNNNNNNNGG	6 cut(s)	421, 422, 1267, 2521, 3424, 3495
AflIII	ACRYGT	2 cut(s)	216, 1429
AhdI	GACNNNNNGTC	1 cut(s)	3181
AjnI	CCWGG	4 cut(s)	1731, 2001, 2691, 3661
AloI	GAACNNNNNNTCC	2 cut(s)	2057, 2089
Alw21I	GWGCWC	2 cut(s)	978, 3432
Alw44I	GTGCAC	1 cut(s)	974
AlwI	GGATC	5 cut(s)	74, 87, 2032, 2136, 2368
AlwNI	CAGNNNCTG	2 cut(s)	248, 3467
Ama87I	CYCGRG	3 cut(s)	36, 1719, 3419
Aor13HI	TCCGGA	2 cut(s)	2323, 3680
AoxI	GGCC	7 cut(s)	1388, 1765, 2078, 2252, 2672, 2791, 3630
ApaLI	GTGCAC	1 cut(s)	974
ApeKI	GCWGC	3 cut(s)	239, 2359, 3479
ApoI	RAATTY	7 cut(s)	19, 95, 559, 779, 788, 1241, 2593
AseI	ATTAAT	1 cut(s)	2928
AspA2I	CCTAGG	1 cut(s)	2669
AspLEI	GCGC	2 cut(s)	283, 1360
AspS9I	GGNCC	6 cut(s)	1497, 1890, 2078, 2673, 2839, 3593
AsuC2I	CCSGG	1 cut(s)	3601
AsuHPI	GGTGA	9 cut(s)	413, 445, 858, 1100, 1281, 1490, 2890, 3251, 3632
AsuII	TTCGAA	1 cut(s)	17
AvaI	CYCGRG	3 cut(s)	36, 1719, 3419
AvaII	GGWCC	4 cut(s)	1497, 1890, 2839, 3593
AvrII	CCTAGG	1 cut(s)	2669
BaeGI	GKGCMC	2 cut(s)	978, 3531
BaeI	ACNNNNGTAYC	2 cut(s)	432, 465
BamHI	GGATCC	1 cut(s)	79
BanI	GGYRCC	1 cut(s)	1965
BbrPI	CACGTG	1 cut(s)	3332
BbsI	GAAGAC	1 cut(s)	1991
Bbv12I	GWGCWC	2 cut(s)	978, 3432
BbvI	GCAGC	3 cut(s)	251, 2371, 3466
BccI	CCATC	3 cut(s)	1358, 2015, 2396
BciT130I	CCWGG	4 cut(s)	1733, 2003, 2693, 3663
BciVI	GTATCC	2 cut(s)	736, 3261
BcnI	CCSGG	1 cut(s)	3601
BfaI	CTAG	3 cut(s)	123, 2670, 3248
BfmI	CTRYAG	2 cut(s)	297, 926
BfuAI	ACCTGC	2 cut(s)	1114, 3604
BfuI	GTATCC	2 cut(s)	736, 3261
BglII	AGATCT	1 cut(s)	3003
BisI	GCNGC	6 cut(s)	11, 169, 240, 542, 2360, 3480
BlnI	CCTAGG	1 cut(s)	2669
BlsI	GCNGC	6 cut(s)	12, 170, 241, 543, 2361, 3481
BmcAI	AGTACT	1 cut(s)	323
Bme1390I	CCNGG	5 cut(s)	1733, 2003, 2693, 3601, 3663
Bme18I	GGWCC	4 cut(s)	1497, 1890, 2839, 3593
BmeRI	GACNNNNNGTC	1 cut(s)	3181
BmeT110I	CYCGRG	3 cut(s)	36, 1719, 3419
BmgT120I	GGNCC	6 cut(s)	1497, 1890, 2078, 2673, 2839, 3593
BmiI	GGNNCC	6 cut(s)	81, 1796, 1967, 2675, 3213, 3288
BmrFI	CCNGG	5 cut(s)	1733, 2003, 2693, 3601, 3663
BmrI	ACTGGG	1 cut(s)	382
BmsI	GCATC	3 cut(s)	226, 660, 975
BmuI	ACTGGG	1 cut(s)	382
BpiI	GAAGAC	1 cut(s)	1991
BplI	GAGNNNNNCTC	2 cut(s)	3287, 3319
BpmI	CTGGAG	3 cut(s)	554, 998, 2543
Bpu14I	TTCGAA	1 cut(s)	17
BpuEI	CTTGAG	3 cut(s)	36, 551, 1103
BpuMI	CCSGG	1 cut(s)	3601
Bsa29I	ATCGAT	1 cut(s)	312
BsaAI	YACGTR	1 cut(s)	3332
BsaHI	GRCGYC	1 cut(s)	771
BsaJI	CCNNGG	7 cut(s)	198, 1493, 2669, 2692, 3404, 3418, 3589
BsaWI	WCCGGW	2 cut(s)	2323, 3680
Bsc4I	CCNNNNNNNGG	6 cut(s)	421, 422, 1267, 2521, 3424, 3495
Bse118I	RCCGGY	1 cut(s)	2080
Bse1I	ACTGG	8 cut(s)	349, 377, 490, 520, 1405, 1862, 2089, 2255
Bse3DI	GCAATG	3 cut(s)	254, 3162, 3395
BseAI	TCCGGA	2 cut(s)	2323, 3680
BseBI	CCWGG	4 cut(s)	1733, 2003, 2693, 3663
BseCI	ATCGAT	1 cut(s)	312
BseDI	CCNNGG	7 cut(s)	198, 1493, 2669, 2692, 3404, 3418, 3589
BseLI	CCNNNNNNNGG	6 cut(s)	421, 422, 1267, 2521, 3424, 3495
BseMI	GCAATG	3 cut(s)	254, 3162, 3395
BseMII	CTCAG	6 cut(s)	339, 618, 2190, 2328, 3596, 3648
BseNI	ACTGG	8 cut(s)	349, 377, 490, 520, 1405, 1862, 2089, 2255
BseRI	GAGGAG	2 cut(s)	2424, 3311
BseSI	GKGCMC	2 cut(s)	978, 3531
BseXI	GCAGC	3 cut(s)	251, 2371, 3466
BseYI	CCCAGC	1 cut(s)	3488
BsgI	GTGCAG	2 cut(s)	1886, 2628
Bsh1236I	CGCG	1 cut(s)	218
BshFI	GGCC	7 cut(s)	1390, 1767, 2080, 2254, 2674, 2793, 3632
BshNI	GGYRCC	1 cut(s)	1965
BshVI	ATCGAT	1 cut(s)	312
BsiHKAI	GWGCWC	2 cut(s)	978, 3432
BsiHKCI	CYCGRG	3 cut(s)	36, 1719, 3419
BsiSI	CCGG	5 cut(s)	2081, 2324, 3600, 3629, 3681
BslFI	GGGAC	4 cut(s)	265, 1122, 1223, 3534
BslI	CCNNNNNNNGG	6 cut(s)	421, 422, 1267, 2521, 3424, 3495
BsmFI	GGGAC	4 cut(s)	265, 1122, 1223, 3534
BsmI	GAATGC	2 cut(s)	2769, 3610
BsnI	GGCC	7 cut(s)	1390, 1767, 2080, 2254, 2674, 2793, 3632
BsoBI	CYCGRG	3 cut(s)	36, 1719, 3419
Bsp119I	TTCGAA	1 cut(s)	17
Bsp1286I	GDGCHC	3 cut(s)	978, 3432, 3531
Bsp13I	TCCGGA	2 cut(s)	2323, 3680
Bsp1407I	TGTACA	1 cut(s)	3196
Bsp19I	CCATGG	1 cut(s)	3404
BspANI	GGCC	7 cut(s)	1390, 1767, 2080, 2254, 2674, 2793, 3632
BspCNI	CTCAG	6 cut(s)	338, 619, 2191, 2329, 3595, 3649
BspDI	ATCGAT	1 cut(s)	312
BspEI	TCCGGA	2 cut(s)	2323, 3680
BspFNI	CGCG	1 cut(s)	218
BspLI	GGNNCC	6 cut(s)	81, 1796, 1967, 2675, 3213, 3288
BspMAI	CTGCAG	1 cut(s)	930
BspMI	ACCTGC	2 cut(s)	1114, 3604
BspPI	GGATC	5 cut(s)	74, 87, 2032, 2136, 2368
BspQI	GCTCTTC	1 cut(s)	1458
BspT104I	TTCGAA	1 cut(s)	17
BspT107I	GGYRCC	1 cut(s)	1965
BsrDI	GCAATG	3 cut(s)	254, 3162, 3395
BsrFI	RCCGGY	1 cut(s)	2080
BsrGI	TGTACA	1 cut(s)	3196
BsrI	ACTGG	8 cut(s)	349, 377, 490, 520, 1405, 1862, 2089, 2255
BssAI	RCCGGY	1 cut(s)	2080
BssECI	CCNNGG	7 cut(s)	198, 1493, 2669, 2692, 3404, 3418, 3589
BssNI	GRCGYC	1 cut(s)	771
BssT1I	CCWWGG	4 cut(s)	198, 2669, 3404, 3589
Bst2UI	CCWGG	4 cut(s)	1733, 2003, 2693, 3663
Bst6I	CTCTTC	5 cut(s)	1332, 1458, 2234, 3063, 3682
BstACI	GRCGYC	1 cut(s)	771
BstAPI	GCANNNNNTGC	2 cut(s)	2862, 3479
BstAUI	TGTACA	1 cut(s)	3196
BstBAI	YACGTR	1 cut(s)	3332
BstBI	TTCGAA	1 cut(s)	17
BstC8I	GCNNGC	4 cut(s)	2151, 2286, 2602, 3610
BstDSI	CCRYGG	2 cut(s)	1493, 3404
BstFNI	CGCG	1 cut(s)	218
BstHHI	GCGC	2 cut(s)	283, 1360
BstMWI	GCNNNNNNNGC	6 cut(s)	10, 39, 1873, 2862, 3479, 3605
BstNI	CCWGG	4 cut(s)	1733, 2003, 2693, 3663
BstNSI	RCATGY	4 cut(s)	8, 640, 1558, 3197
BstSCI	CCNGG	5 cut(s)	1731, 2001, 2691, 3599, 3661
BstSFI	CTRYAG	2 cut(s)	297, 926
BstSLI	GKGCMC	2 cut(s)	978, 3531
BstUI	CGCG	1 cut(s)	218
BstV1I	GCAGC	3 cut(s)	251, 2371, 3466
BstV2I	GAAGAC	1 cut(s)	1991
BstX2I	RGATCY	4 cut(s)	79, 2128, 2373, 3003
BstXI	CCANNNNNNTGG	1 cut(s)	267
BstYI	RGATCY	4 cut(s)	79, 2128, 2373, 3003
Bsu15I	ATCGAT	1 cut(s)	312
BsuI	GTATCC	2 cut(s)	736, 3261
BsuRI	GGCC	7 cut(s)	1390, 1767, 2080, 2254, 2674, 2793, 3632
BsuTUI	ATCGAT	1 cut(s)	312
BtgI	CCRYGG	2 cut(s)	1493, 3404
BtsI	GCAGTG	1 cut(s)	2640
BtsIMutI	CAGTG	9 cut(s)	700, 957, 1212, 1412, 1869, 2096, 2640, 3126, 3654
BveI	ACCTGC	2 cut(s)	1114, 3604
Cac8I	GCNNGC	4 cut(s)	2151, 2286, 2602, 3610
CaiI	CAGNNNCTG	2 cut(s)	248, 3467
CfoI	GCGC	2 cut(s)	283, 1360
Cfr10I	RCCGGY	1 cut(s)	2080
Cfr13I	GGNCC	6 cut(s)	1497, 1890, 2078, 2673, 2839, 3593
ClaI	ATCGAT	1 cut(s)	312
CseI	GACGC	1 cut(s)	224
DraI	TTTAAA	1 cut(s)	175
DriI	GACNNNNNGTC	1 cut(s)	3181
Eam1104I	CTCTTC	5 cut(s)	1332, 1458, 2234, 3063, 3682
Eam1105I	GACNNNNNGTC	1 cut(s)	3181
EarI	CTCTTC	5 cut(s)	1332, 1458, 2234, 3063, 3682
EciI	GGCGGA	2 cut(s)	196, 2136
Eco130I	CCWWGG	4 cut(s)	198, 2669, 3404, 3589
Eco147I	AGGCCT	1 cut(s)	2793
Eco32I	GATATC	2 cut(s)	428, 2170
Eco47I	GGWCC	4 cut(s)	1497, 1890, 2839, 3593
Eco57I	CTGAAG	5 cut(s)	627, 1160, 1510, 3273, 3489
Eco72I	CACGTG	1 cut(s)	3332
Eco88I	CYCGRG	3 cut(s)	36, 1719, 3419
EcoO109I	RGGNCCY	2 cut(s)	2673, 3593
EcoRI	GAATTC	1 cut(s)	559
EcoRII	CCWGG	4 cut(s)	1731, 2001, 2691, 3661
EcoRV	GATATC	2 cut(s)	428, 2170
EcoT14I	CCWWGG	4 cut(s)	198, 2669, 3404, 3589
EcoT22I	ATGCAT	3 cut(s)	6, 73, 1552
ErhI	CCWWGG	4 cut(s)	198, 2669, 3404, 3589
FalI	AAGNNNNNCTT	6 cut(s)	640, 672, 2157, 2189, 3299, 3331
FaqI	GGGAC	4 cut(s)	265, 1122, 1223, 3534
FauI	CCCGC	3 cut(s)	1099, 1200, 3276
FauNDI	CATATG	3 cut(s)	262, 496, 615
FblI	GTMKAC	1 cut(s)	1773
Fnu4HI	GCNGC	6 cut(s)	11, 169, 240, 542, 2360, 3480
Fsp4HI	GCNGC	6 cut(s)	11, 169, 240, 542, 2360, 3480
FspBI	CTAG	3 cut(s)	123, 2670, 3248
FspI	TGCGCA	1 cut(s)	282
GlaI	GCGC	2 cut(s)	282, 1359
GluI	GCNGC	6 cut(s)	11, 169, 240, 542, 2360, 3480
GsaI	CCCAGC	1 cut(s)	3492
GsuI	CTGGAG	3 cut(s)	554, 998, 2543
HaeIII	GGCC	7 cut(s)	1390, 1767, 2080, 2254, 2674, 2793, 3632
HapII	CCGG	5 cut(s)	2081, 2324, 3600, 3629, 3681
HgaI	GACGC	1 cut(s)	224
HhaI	GCGC	2 cut(s)	283, 1360
Hin1I	GRCGYC	1 cut(s)	771
Hin6I	GCGC	2 cut(s)	281, 1358
HinP1I	GCGC	2 cut(s)	281, 1358
HincII	GTYRAC	3 cut(s)	1774, 2721, 3086
HindII	GTYRAC	3 cut(s)	1774, 2721, 3086
HindIII	AAGCTT	4 cut(s)	683, 803, 2186, 2564
HpaI	GTTAAC	1 cut(s)	2721
HpaII	CCGG	5 cut(s)	2081, 2324, 3600, 3629, 3681
HphI	GGTGA	9 cut(s)	413, 445, 858, 1100, 1281, 1490, 2890, 3251, 3632
Hpy166II	GTNNAC	6 cut(s)	214, 976, 1774, 2721, 3086, 3109
Hpy8I	GTNNAC	6 cut(s)	214, 976, 1774, 2721, 3086, 3109
HpyF10VI	GCNNNNNNNGC	6 cut(s)	10, 39, 1873, 2862, 3479, 3605
Hsp92I	GRCGYC	1 cut(s)	771
HspAI	GCGC	2 cut(s)	281, 1358
Kpn2I	TCCGGA	2 cut(s)	2323, 3680
KspAI	GTTAAC	1 cut(s)	2721
LguI	GCTCTTC	1 cut(s)	1458
LmnI	GCTCC	6 cut(s)	58, 2356, 2696, 3022, 3409, 3427
Lsp1109I	GCAGC	3 cut(s)	251, 2371, 3466
LweI	GCATC	3 cut(s)	226, 660, 975
MaeI	CTAG	3 cut(s)	123, 2670, 3248
MfeI	CAATTG	1 cut(s)	108
MflI	RGATCY	4 cut(s)	79, 2128, 2373, 3003
MhlI	GDGCHC	3 cut(s)	978, 3432, 3531
MluI	ACGCGT	1 cut(s)	216
MlyI	GAGTC	3 cut(s)	494, 1007, 2631
MmeI	TCCRAC	5 cut(s)	108, 1987, 2766, 3384, 3405
Mph1103I	ATGCAT	3 cut(s)	6, 73, 1552
MroI	TCCGGA	2 cut(s)	2323, 3680
MslI	CAYNNNNRTG	9 cut(s)	265, 971, 1350, 1559, 1862, 2442, 2574, 2964, 3237
MspA1I	CMGCKG	1 cut(s)	541
MspI	CCGG	5 cut(s)	2081, 2324, 3600, 3629, 3681
MspR9I	CCNGG	5 cut(s)	1733, 2003, 2693, 3601, 3663
MunI	CAATTG	1 cut(s)	108
Mva1269I	GAATGC	2 cut(s)	2769, 3610
MvaI	CCWGG	4 cut(s)	1733, 2003, 2693, 3663
MvnI	CGCG	1 cut(s)	218
MwoI	GCNNNNNNNGC	6 cut(s)	10, 39, 1873, 2862, 3479, 3605
NciI	CCSGG	1 cut(s)	3601
NcoI	CCATGG	1 cut(s)	3404
NdeI	CATATG	3 cut(s)	262, 496, 615
NlaIV	GGNNCC	6 cut(s)	81, 1796, 1967, 2675, 3213, 3288
NmuCI	GTSAC	7 cut(s)	368, 695, 1066, 1088, 1432, 3124, 3327
NsbI	TGCGCA	1 cut(s)	282
NsiI	ATGCAT	3 cut(s)	6, 73, 1552
NspI	RCATGY	4 cut(s)	8, 640, 1558, 3197
NspV	TTCGAA	1 cut(s)	17
PaeR7I	CTCGAG	1 cut(s)	1719
PceI	AGGCCT	1 cut(s)	2793
PciSI	GCTCTTC	1 cut(s)	1458
PcsI	WCGNNNNNNNCGW	2 cut(s)	1921, 2212
PctI	GAATGC	2 cut(s)	2769, 3610
PflFI	GACNNNGTC	2 cut(s)	727, 2837
PkrI	GCNGC	6 cut(s)	12, 170, 241, 543, 2361, 3481
PleI	GAGTC	3 cut(s)	494, 1006, 2631
PmaCI	CACGTG	1 cut(s)	3332
PmlI	CACGTG	1 cut(s)	3332
PpsI	GAGTC	3 cut(s)	494, 1006, 2631
Ppu21I	YACGTR	1 cut(s)	3332
PpuMI	RGGWCCY	1 cut(s)	3593
PshBI	ATTAAT	1 cut(s)	2928
PsiI	TTATAA	1 cut(s)	881
Psp1406I	AACGTT	2 cut(s)	670, 2466
Psp5II	RGGWCCY	1 cut(s)	3593
Psp6I	CCWGG	4 cut(s)	1731, 2001, 2691, 3661
PspCI	CACGTG	1 cut(s)	3332
PspFI	CCCAGC	1 cut(s)	3488
PspGI	CCWGG	4 cut(s)	1731, 2001, 2691, 3661
PspN4I	GGNNCC	6 cut(s)	81, 1796, 1967, 2675, 3213, 3288
PspPI	GGNCC	6 cut(s)	1497, 1890, 2078, 2673, 2839, 3593
PspPPI	RGGWCCY	1 cut(s)	3593
PstI	CTGCAG	1 cut(s)	930
PstNI	CAGNNNCTG	2 cut(s)	248, 3467
PsuI	RGATCY	4 cut(s)	79, 2128, 2373, 3003
PsyI	GACNNNGTC	2 cut(s)	727, 2837
RseI	CAYNNNNRTG	9 cut(s)	265, 971, 1350, 1559, 1862, 2442, 2574, 2964, 3237
SalI	GTCGAC	1 cut(s)	1772
SapI	GCTCTTC	1 cut(s)	1458
SatI	GCNGC	6 cut(s)	11, 169, 240, 542, 2360, 3480
Sau96I	GGNCC	6 cut(s)	1497, 1890, 2078, 2673, 2839, 3593
ScaI	AGTACT	1 cut(s)	323
SchI	GAGTC	3 cut(s)	494, 1007, 2631
ScrFI	CCNGG	5 cut(s)	1733, 2003, 2693, 3601, 3663
SduI	GDGCHC	3 cut(s)	978, 3432, 3531
SfaNI	GCATC	3 cut(s)	226, 660, 975
SfcI	CTRYAG	2 cut(s)	297, 926
Sfr274I	CTCGAG	1 cut(s)	1719
SfuI	TTCGAA	1 cut(s)	17
SinI	GGWCC	4 cut(s)	1497, 1890, 2839, 3593
SlaI	CTCGAG	1 cut(s)	1719
SmiMI	CAYNNNNRTG	9 cut(s)	265, 971, 1350, 1559, 1862, 2442, 2574, 2964, 3237
SmlI	CTYRAG	4 cut(s)	51, 530, 1082, 1719
SmoI	CTYRAG	4 cut(s)	51, 530, 1082, 1719
SseBI	AGGCCT	1 cut(s)	2793
SspMI	CTAG	3 cut(s)	123, 2670, 3248
StuI	AGGCCT	1 cut(s)	2793
StyD4I	CCNGG	5 cut(s)	1731, 2001, 2691, 3599, 3661
StyI	CCWWGG	4 cut(s)	198, 2669, 3404, 3589
TaqI	TCGA	8 cut(s)	17, 312, 1056, 1643, 1720, 1773, 2383, 3511
TatI	WGTACW	5 cut(s)	321, 2751, 2932, 3130, 3196
TauI	GCSGC	3 cut(s)	13, 171, 544
TscAI	CASTG	9 cut(s)	700, 964, 1219, 1412, 1869, 2096, 2647, 3133, 3661
TseFI	GTSAC	7 cut(s)	368, 695, 1066, 1088, 1432, 3124, 3327
TseI	GCWGC	3 cut(s)	239, 2359, 3479
Tsp45I	GTSAC	7 cut(s)	368, 695, 1066, 1088, 1432, 3124, 3327
TspGWI	ACGGA	6 cut(s)	461, 1510, 1787, 2504, 2831, 3280
TspRI	CASTG	9 cut(s)	700, 964, 1219, 1412, 1869, 2096, 2647, 3133, 3661
Tth111I	GACNNNGTC	2 cut(s)	727, 2837
VneI	GTGCAC	1 cut(s)	974
VpaK11BI	GGWCC	4 cut(s)	1497, 1890, 2839, 3593
VspI	ATTAAT	1 cut(s)	2928
XapI	RAATTY	7 cut(s)	19, 95, 559, 779, 788, 1241, 2593
XceI	RCATGY	4 cut(s)	8, 640, 1558, 3197
XhoI	CTCGAG	1 cut(s)	1719
XmaJI	CCTAGG	1 cut(s)	2669
XmiI	GTMKAC	1 cut(s)	1773
XspI	CTAG	3 cut(s)	123, 2670, 3248
ZraI	GACGTC	1 cut(s)	772
ZrmI	AGTACT	1 cut(s)	323
Zsp2I	ATGCAT	3 cut(s)	6, 73, 1552

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.