Ubiquitin carboxyl-terminal hydrolase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 275 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 883 |
| AccII | CGCG | 1 cut(s) | 1395 |
| AciI | CCGC | 4 cut(s) | 140, 433, 794, 967 |
| AclWI | GGATC | 1 cut(s) | 1346 |
| AcsI | RAATTY | 3 cut(s) | 359, 565, 675 |
| AfaI | GTAC | 7 cut(s) | 35, 1115, 1260, 1275, 1382, 1579, 1595 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 883, 1635 |
| AgsI | TTSAA | 7 cut(s) | 397, 609, 637, 674, 800, 917, 1483 |
| AjnI | CCWGG | 2 cut(s) | 297, 502 |
| AluBI | AGCT | 6 cut(s) | 177, 239, 928, 962, 1101, 1279 |
| AluI | AGCT | 6 cut(s) | 177, 239, 928, 962, 1101, 1279 |
| Alw21I | GWGCWC | 2 cut(s) | 855, 1238 |
| Alw26I | GTCTC | 3 cut(s) | 174, 457, 1287 |
| AlwI | GGATC | 1 cut(s) | 1346 |
| AoxI | GGCC | 3 cut(s) | 456, 571, 1154 |
| ApoI | RAATTY | 3 cut(s) | 359, 565, 675 |
| AseI | ATTAAT | 2 cut(s) | 1254, 1371 |
| AspLEI | GCGC | 2 cut(s) | 1395, 1397 |
| AspS9I | GGNCC | 3 cut(s) | 295, 456, 571 |
| AsuC2I | CCSGG | 1 cut(s) | 863 |
| AsuHPI | GGTGA | 2 cut(s) | 956, 1016 |
| AsuII | TTCGAA | 1 cut(s) | 1550 |
| AvaII | GGWCC | 1 cut(s) | 295 |
| BaeGI | GKGCMC | 1 cut(s) | 252 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1265, 1298 |
| Bbv12I | GWGCWC | 2 cut(s) | 855, 1238 |
| BccI | CCATC | 4 cut(s) | 495, 755, 889, 1483 |
| BceAI | ACGGC | 1 cut(s) | 558 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 574, 608 |
| BciT130I | CCWGG | 2 cut(s) | 299, 504 |
| BcnI | CCSGG | 1 cut(s) | 863 |
| BcoDI | GTCTC | 3 cut(s) | 174, 457, 1287 |
| BfmI | CTRYAG | 1 cut(s) | 1333 |
| BglII | AGATCT | 1 cut(s) | 1329 |
| BlpI | GCTNAGC | 1 cut(s) | 240 |
| BmcAI | AGTACT | 1 cut(s) | 1382 |
| Bme1390I | CCNGG | 3 cut(s) | 299, 504, 863 |
| Bme18I | GGWCC | 1 cut(s) | 295 |
| BmgT120I | GGNCC | 3 cut(s) | 295, 456, 571 |
| BmiI | GGNNCC | 1 cut(s) | 1610 |
| BmrFI | CCNGG | 3 cut(s) | 299, 504, 863 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1032 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 240 |
| Bpu14I | TTCGAA | 1 cut(s) | 1550 |
| BpuEI | CTTGAG | 1 cut(s) | 1331 |
| BpuMI | CCSGG | 1 cut(s) | 863 |
| BsaJI | CCNNGG | 1 cut(s) | 503 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 883, 1635 |
| Bse1I | ACTGG | 2 cut(s) | 614, 1378 |
| Bse3DI | GCAATG | 1 cut(s) | 216 |
| BseBI | CCWGG | 2 cut(s) | 299, 504 |
| BseDI | CCNNGG | 1 cut(s) | 503 |
| BseGI | GGATG | 3 cut(s) | 818, 1252, 1285 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 883, 1635 |
| BseMI | GCAATG | 1 cut(s) | 216 |
| BseMII | CTCAG | 2 cut(s) | 187, 254 |
| BseNI | ACTGG | 2 cut(s) | 614, 1378 |
| BsePI | GCGCGC | 1 cut(s) | 1393 |
| BseRI | GAGGAG | 5 cut(s) | 109, 216, 416, 914, 1415 |
| BseSI | GKGCMC | 1 cut(s) | 252 |
| BsgI | GTGCAG | 1 cut(s) | 990 |
| Bsh1236I | CGCG | 1 cut(s) | 1395 |
| Bsh1285I | CGRYCG | 1 cut(s) | 746 |
| BshFI | GGCC | 3 cut(s) | 458, 573, 1156 |
| BsiEI | CGRYCG | 1 cut(s) | 746 |
| BsiHKAI | GWGCWC | 2 cut(s) | 855, 1238 |
| BsiSI | CCGG | 1 cut(s) | 862 |
| BslFI | GGGAC | 2 cut(s) | 540, 779 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 883, 1635 |
| BsmAI | GTCTC | 3 cut(s) | 174, 457, 1287 |
| BsmBI | CGTCTC | 1 cut(s) | 174 |
| BsmFI | GGGAC | 2 cut(s) | 540, 779 |
| BsmI | GAATGC | 1 cut(s) | 196 |
| BsnI | GGCC | 3 cut(s) | 458, 573, 1156 |
| Bsp119I | TTCGAA | 1 cut(s) | 1550 |
| Bsp1286I | GDGCHC | 3 cut(s) | 252, 855, 1238 |
| Bsp1407I | TGTACA | 1 cut(s) | 1577 |
| Bsp143I | GATC | 4 cut(s) | 216, 340, 1329, 1338 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 240 |
| BspACI | CCGC | 4 cut(s) | 140, 433, 794, 967 |
| BspANI | GGCC | 3 cut(s) | 458, 573, 1156 |
| BspCNI | CTCAG | 2 cut(s) | 186, 253 |
| BspFNI | CGCG | 1 cut(s) | 1395 |
| BspHI | TCATGA | 2 cut(s) | 67, 343 |
| BspLI | GGNNCC | 1 cut(s) | 1610 |
| BspPI | GGATC | 1 cut(s) | 1346 |
| BspT104I | TTCGAA | 1 cut(s) | 1550 |
| BsrDI | GCAATG | 1 cut(s) | 216 |
| BsrGI | TGTACA | 1 cut(s) | 1577 |
| BsrI | ACTGG | 2 cut(s) | 614, 1378 |
| BssECI | CCNNGG | 1 cut(s) | 503 |
| BssHII | GCGCGC | 1 cut(s) | 1393 |
| BssMI | GATC | 4 cut(s) | 216, 340, 1329, 1338 |
| Bst2UI | CCWGG | 2 cut(s) | 299, 504 |
| Bst4CI | ACNGT | 9 cut(s) | 56, 100, 159, 1214, 1240, 1352, 1477, 1516, 1540 |
| Bst6I | CTCTTC | 2 cut(s) | 593, 1410 |
| BstAUI | TGTACA | 1 cut(s) | 1577 |
| BstBI | TTCGAA | 1 cut(s) | 1550 |
| BstC8I | GCNNGC | 3 cut(s) | 140, 587, 1395 |
| BstDEI | CTNAG | 3 cut(s) | 173, 240, 1032 |
| BstF5I | GGATG | 3 cut(s) | 818, 1252, 1285 |
| BstFNI | CGCG | 1 cut(s) | 1395 |
| BstHHI | GCGC | 2 cut(s) | 1395, 1397 |
| BstKTI | GATC | 4 cut(s) | 219, 343, 1332, 1341 |
| BstMAI | GTCTC | 3 cut(s) | 174, 457, 1287 |
| BstMBI | GATC | 4 cut(s) | 216, 340, 1329, 1338 |
| BstMCI | CGRYCG | 1 cut(s) | 746 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 439, 1440 |
| BstNI | CCWGG | 2 cut(s) | 299, 504 |
| BstSCI | CCNGG | 3 cut(s) | 297, 502, 861 |
| BstSFI | CTRYAG | 1 cut(s) | 1333 |
| BstSLI | GKGCMC | 1 cut(s) | 252 |
| BstUI | CGCG | 1 cut(s) | 1395 |
| BstX2I | RGATCY | 2 cut(s) | 1329, 1338 |
| BstYI | RGATCY | 2 cut(s) | 1329, 1338 |
| BsuRI | GGCC | 3 cut(s) | 458, 573, 1156 |
| BtsCI | GGATG | 3 cut(s) | 818, 1252, 1285 |
| BtsI | GCAGTG | 1 cut(s) | 1002 |
| BtsIMutI | CAGTG | 7 cut(s) | 155, 180, 528, 1002, 1236, 1357, 1473 |
| Cac8I | GCNNGC | 3 cut(s) | 140, 587, 1395 |
| CciI | TCATGA | 2 cut(s) | 67, 343 |
| CfoI | GCGC | 2 cut(s) | 1395, 1397 |
| Cfr13I | GGNCC | 3 cut(s) | 295, 456, 571 |
| Csp6I | GTAC | 7 cut(s) | 34, 1114, 1259, 1274, 1381, 1578, 1594 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 778, 813 |
| CviAII | CATG | 6 cut(s) | 68, 344, 709, 1184, 1451, 1630 |
| CviQI | GTAC | 7 cut(s) | 34, 1114, 1259, 1274, 1381, 1578, 1594 |
| DdeI | CTNAG | 3 cut(s) | 173, 240, 1032 |
| DpnI | GATC | 4 cut(s) | 218, 342, 1331, 1340 |
| DpnII | GATC | 4 cut(s) | 216, 340, 1329, 1338 |
| Eam1104I | CTCTTC | 2 cut(s) | 593, 1410 |
| EarI | CTCTTC | 2 cut(s) | 593, 1410 |
| Eco47I | GGWCC | 1 cut(s) | 295 |
| EcoO109I | RGGNCCY | 1 cut(s) | 295 |
| EcoRII | CCWGG | 2 cut(s) | 297, 502 |
| Esp3I | CGTCTC | 1 cut(s) | 174 |
| FaeI | CATG | 6 cut(s) | 71, 347, 712, 1187, 1454, 1633 |
| FaqI | GGGAC | 2 cut(s) | 540, 779 |
| FatI | CATG | 6 cut(s) | 67, 343, 708, 1183, 1450, 1629 |
| FauI | CCCGC | 1 cut(s) | 147 |
| FokI | GGATG | 3 cut(s) | 825, 1259, 1272 |
| GlaI | GCGC | 2 cut(s) | 1394, 1396 |
| HaeIII | GGCC | 3 cut(s) | 458, 573, 1156 |
| HapII | CCGG | 1 cut(s) | 862 |
| HhaI | GCGC | 2 cut(s) | 1395, 1397 |
| Hin1II | CATG | 6 cut(s) | 71, 347, 712, 1187, 1454, 1633 |
| Hin6I | GCGC | 2 cut(s) | 1393, 1395 |
| HinP1I | GCGC | 2 cut(s) | 1393, 1395 |
| HincII | GTYRAC | 2 cut(s) | 103, 1083 |
| HindII | GTYRAC | 2 cut(s) | 103, 1083 |
| HinfI | GANTC | 8 cut(s) | 116, 512, 637, 999, 1207, 1405, 1447, 1552 |
| HpaI | GTTAAC | 1 cut(s) | 1083 |
| HpaII | CCGG | 1 cut(s) | 862 |
| HphI | GGTGA | 2 cut(s) | 956, 1016 |
| Hpy166II | GTNNAC | 7 cut(s) | 25, 34, 103, 1083, 1456, 1501, 1596 |
| Hpy188I | TCNGA | 3 cut(s) | 783, 998, 1105 |
| Hpy188III | TCNNGA | 7 cut(s) | 68, 287, 344, 371, 448, 595, 1465 |
| Hpy8I | GTNNAC | 7 cut(s) | 25, 34, 103, 1083, 1456, 1501, 1596 |
| Hpy99I | CGWCG | 1 cut(s) | 1443 |
| HpyAV | CCTTC | 6 cut(s) | 158, 309, 803, 843, 1264, 1627 |
| HpyCH4III | ACNGT | 9 cut(s) | 56, 100, 159, 1214, 1240, 1352, 1477, 1516, 1540 |
| HpyCH4IV | ACGT | 2 cut(s) | 83, 233 |
| HpyCH4V | TGCA | 3 cut(s) | 410, 556, 1007 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 439, 1440 |
| HpyF3I | CTNAG | 3 cut(s) | 173, 240, 1032 |
| HpySE526I | ACGT | 2 cut(s) | 83, 233 |
| Hsp92II | CATG | 6 cut(s) | 71, 347, 712, 1187, 1454, 1633 |
| HspAI | GCGC | 2 cut(s) | 1393, 1395 |
| KspAI | GTTAAC | 1 cut(s) | 1083 |
| Kzo9I | GATC | 4 cut(s) | 216, 340, 1329, 1338 |
| LmnI | GCTCC | 1 cut(s) | 182 |
| MaeII | ACGT | 2 cut(s) | 83, 233 |
| MaeIII | GTNAC | 1 cut(s) | 1471 |
| MalI | GATC | 4 cut(s) | 218, 342, 1331, 1340 |
| MboI | GATC | 4 cut(s) | 216, 340, 1329, 1338 |
| MboII | GAAGA | 7 cut(s) | 521, 574, 597, 610, 677, 1333, 1427 |
| MflI | RGATCY | 2 cut(s) | 1329, 1338 |
| MhlI | GDGCHC | 3 cut(s) | 252, 855, 1238 |
| MlyI | GAGTC | 2 cut(s) | 110, 993 |
| MmeI | TCCRAC | 1 cut(s) | 254 |
| MseI | TTAA | 7 cut(s) | 387, 1011, 1070, 1082, 1254, 1263, 1371 |
| MslI | CAYNNNNRTG | 2 cut(s) | 428, 936 |
| MspA1I | CMGCKG | 1 cut(s) | 1279 |
| MspI | CCGG | 1 cut(s) | 862 |
| MspR9I | CCNGG | 3 cut(s) | 299, 504, 863 |
| Mva1269I | GAATGC | 1 cut(s) | 196 |
| MvaI | CCWGG | 2 cut(s) | 299, 504 |
| MvnI | CGCG | 1 cut(s) | 1395 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 439, 1440 |
| NciI | CCSGG | 1 cut(s) | 863 |
| NdeII | GATC | 4 cut(s) | 216, 340, 1329, 1338 |
| NlaIII | CATG | 6 cut(s) | 71, 347, 712, 1187, 1454, 1633 |
| NlaIV | GGNNCC | 1 cut(s) | 1610 |
| NmuCI | GTSAC | 1 cut(s) | 1471 |
| NspV | TTCGAA | 1 cut(s) | 1550 |
| PagI | TCATGA | 2 cut(s) | 67, 343 |
| PauI | GCGCGC | 1 cut(s) | 1393 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 361, 487, 743 |
| PctI | GAATGC | 1 cut(s) | 196 |
| PfeI | GAWTC | 6 cut(s) | 512, 637, 1207, 1405, 1447, 1552 |
| PflFI | GACNNNGTC | 1 cut(s) | 335 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 883 |
| PleI | GAGTC | 2 cut(s) | 110, 993 |
| PpsI | GAGTC | 2 cut(s) | 110, 993 |
| PpuMI | RGGWCCY | 1 cut(s) | 295 |
| PshBI | ATTAAT | 2 cut(s) | 1254, 1371 |
| PsiI | TTATAA | 1 cut(s) | 275 |
| Psp5II | RGGWCCY | 1 cut(s) | 295 |
| Psp6I | CCWGG | 2 cut(s) | 297, 502 |
| PspGI | CCWGG | 2 cut(s) | 297, 502 |
| PspN4I | GGNNCC | 1 cut(s) | 1610 |
| PspPI | GGNCC | 3 cut(s) | 295, 456, 571 |
| PspPPI | RGGWCCY | 1 cut(s) | 295 |
| PsuI | RGATCY | 2 cut(s) | 1329, 1338 |
| PsyI | GACNNNGTC | 1 cut(s) | 335 |
| PteI | GCGCGC | 1 cut(s) | 1393 |
| PvuII | CAGCTG | 1 cut(s) | 1279 |
| RsaI | GTAC | 7 cut(s) | 35, 1115, 1260, 1275, 1382, 1579, 1595 |
| RsaNI | GTAC | 7 cut(s) | 34, 1114, 1259, 1274, 1381, 1578, 1594 |
| RseI | CAYNNNNRTG | 2 cut(s) | 428, 936 |
| SaqAI | TTAA | 7 cut(s) | 387, 1011, 1070, 1082, 1254, 1263, 1371 |
| Sau3AI | GATC | 4 cut(s) | 216, 340, 1329, 1338 |
| Sau96I | GGNCC | 3 cut(s) | 295, 456, 571 |
| ScaI | AGTACT | 1 cut(s) | 1382 |
| SchI | GAGTC | 2 cut(s) | 110, 993 |
| ScrFI | CCNGG | 3 cut(s) | 299, 504, 863 |
| SduI | GDGCHC | 3 cut(s) | 252, 855, 1238 |
| SfcI | CTRYAG | 1 cut(s) | 1333 |
| SfuI | TTCGAA | 1 cut(s) | 1550 |
| SinI | GGWCC | 1 cut(s) | 295 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 428, 936 |
| SmlI | CTYRAG | 1 cut(s) | 1310 |
| SmoI | CTYRAG | 1 cut(s) | 1310 |
| SsiI | CCGC | 4 cut(s) | 140, 433, 794, 967 |
| SspI | AATATT | 1 cut(s) | 1369 |
| StyD4I | CCNGG | 3 cut(s) | 297, 502, 861 |
| TaaI | ACNGT | 9 cut(s) | 56, 100, 159, 1214, 1240, 1352, 1477, 1516, 1540 |
| TaiI | ACGT | 2 cut(s) | 86, 236 |
| TaqI | TCGA | 7 cut(s) | 203, 286, 339, 490, 746, 1385, 1550 |
| TaqII | GACCGA | 2 cut(s) | 732, 1545 |
| TatI | WGTACW | 5 cut(s) | 1113, 1258, 1380, 1577, 1593 |
| TfiI | GAWTC | 6 cut(s) | 512, 637, 1207, 1405, 1447, 1552 |
| Tru1I | TTAA | 7 cut(s) | 387, 1011, 1070, 1082, 1254, 1263, 1371 |
| Tru9I | TTAA | 7 cut(s) | 387, 1011, 1070, 1082, 1254, 1263, 1371 |
| TscAI | CASTG | 7 cut(s) | 162, 187, 528, 1009, 1243, 1357, 1480 |
| TseFI | GTSAC | 1 cut(s) | 1471 |
| Tsp45I | GTSAC | 1 cut(s) | 1471 |
| TspDTI | ATGAA | 7 cut(s) | 152, 678, 825, 860, 1134, 1439, 1646 |
| TspGWI | ACGGA | 1 cut(s) | 353 |
| TspRI | CASTG | 7 cut(s) | 162, 187, 528, 1009, 1243, 1357, 1480 |
| Tth111I | GACNNNGTC | 1 cut(s) | 335 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 883 |
| VpaK11BI | GGWCC | 1 cut(s) | 295 |
| VspI | ATTAAT | 2 cut(s) | 1254, 1371 |
| XapI | RAATTY | 3 cut(s) | 359, 565, 675 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 758 |
| ZrmI | AGTACT | 1 cut(s) | 1382 |