Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0000333

Physical Location & Seq

Genomic Coordinates Forward (+)

27209189 .. 27220880

Gene Structure 11692 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023698

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3078 bp

ATGACTATGATGACTCCTCCACCTGTAGATCAAGAGGACGAAGAGATGCTGGTGCCGCATACGGATTTGGTCGAAGGTCCTCAGCCCATGGAAGTAGCTCAAGTGGAGCCAGCAAGTACAGTTGATAATCAGCCAGTGGAGGAGCCTCCAACCATGAAATTCACTTGGACAATTGAGAGCTTTTCTAGGTTGAACACCAAAAAGCACTACTCTGACATGTTCATCGTCGGTGGTTATAAATGGCGGATACTGATTTTTCCTAAAGGAAACAATGTGGACTACTTGTCAATGTATTTGGATGTGGCGGATTCGCAGGCATTGCCTTATGGTTGGAGTAGATATGCGCAGTTTAGCTTGGCTATATGTCATTTAGAAGTTACTCTTGAAATGGACATCCTTGAGACTTTGGATGTAACTGAGCTCTCTGGCATACTGAAGGGATATGATATTGCTGTGTATCATATGCCAACAACTGAGAATGACATGCCTTCAGGAAGCATCCCTTTGGCTCTTCAAAGTTTATTCTATAAGCTTCAATACAACGAGAGCAGTGTTGCAACTAAAGAATTAACCAAGTCTTTTGGATGGGATACATATGATTCGTTCATGCAACATGATGTGCAGGAACTCAATAGAGTTCTATGTGAAAAGCTTGAGGATAAAATGAAGGGAACTGTGGTGGAGGGTACAATACAGCAGTTATTTGAAGGACATCACATGAATTACATTGAATGCATCAATGTGGACTACAAATCTACAAGAAAAGAGTCATTTTATGATCTCCAGCTTGATGTAAAAGGATGTCGTGATGTTTACGCTTCTTTTGACAAGTATGTGGAAGTTGAACGTCTTGAGGGTGACAATAAGTACCATGCAGAAGAACATGGTTTGCAGGATGCTAAGAAGGGTGTCCTGTTTATTGACTTCCCTCCAGTTCTTCAGCTTCAGCTAAAGCGATTTGAGTATGATTTTATGAGGGACACTATGGTAAAGATAAATGATCGCTATGAATTTCCTCTTCAACTTGACCTTGATAGGGAGAATGGCAAATATCTGTCACCTGAATCAGATAAGAGTGTGCGGAACCTCTACACTCTTCATAGTGTCTTAGTTCATAGTGGTGGCGTGCATGGAGGGCATTATTATGCTTTTATCAGGCCAACCCTTACTGATCAGTGGTATAAGTTTGATGATGAACGCGTGACAAAAGAGGATGTGAAGAGGGCATTAGAGGAACAGTATGGTGGTGAGGAAGAGATGCCCCAGACCAATCCTGGTTTTAATAATACTCCTTTCAAGTTTACAAAATACTCGAACGCATACATGCTTGTGTACATCCGGGACAGTGACAAGGACAAAATAATATGTGATGTGGATCAGAAGGACATAGCTGAACACCTAAGGATAAGGTTGAAGAAAGAACAAGAGGAGAAGGAGGACAAGAGGAGGTATAAAGCACAGGCCCATCTTTATACTATTATTAAGGTTGCACGAGATGAGGACCTAGCAGAACAAATTGGAAGGGACATATATTTTGACCTTGTGGACCATGACAAAGTCCGTAGTTTTCGAATCCAGAAACAGACGCCCTTTAACCTTTTCAAGGAGGAAGTTGCGAAAGAGTTGGGGATACCAGTGCAATTTCAGCGATTCTGGATTTGGGCCAAGAGGCAAAACCACACATATCGTCCCAACCGACCATTGACACCCCAGGAAGAATTACAAACAGTTGGACAATTGAGGGAGGCATCAAATAAAACACACAATGCAGAGCTCAAGTTGTTTTTGGAAGTAGAGCTTGGGCCGGAGAAAATTCCTATTCCTCCACCAGACAAAACCAAGGATGATATTCTGCTTTTCTTTAAGTTTTATGAACCTGAGAAACGAGAATTACGGTTTGTTGGTCGGCTTTTTGTTAAGAGTTCTACCAAGCCAATAGAAATAATAGCAAAAATTAATCAAATGGCTGGTTTTGCTCCTGATGAAGAAATTGAACTCTATGAGGAAATAAAGTTTGAGCCTTGTATCATGTGTGAACATCTTGACAAGAGGACCTCATTTCGATTAAGTCAGATTGAAGATGGGGACATTATATGCTTTCAAAAACCTACTCCACTTGAAAGTGAAGAATGTAAATATCCTGACGTCCCTTCATTTTTGGAATATGTACACAACCGTCAGATTGTTCATTTTCGCTCTTTGGAGAAACCAAAAGAGGATGATTTTTCTTTAGAATTGTCAAAGCTACACACTTATGATGATGTGGTGGAGAAGGTGGCTTCCCAAATTGGTTTGGATGATCCTACCAAAATCAGACTCACTGCGCATAACTGCTATTCTCAACAACCTAAACCTCAACCCATCAAATATCGAGGAGTAGAGCATTTAACAGATATGCTGGTCCACTATAATCAAAGTTCTGATATTTTGTATTATGAAGTCTTGGACATCCCCCTGCCAGAATTACAAGGCCTAAAAAATCTGAAAGTTGCATTTCATCATGCTACAAAAGATGAGGTGGTGATTCACAATATAAGATTGCCCAAGCAGAGCACTGTTGGGGATGTGATTAATGTGCTAAAAACGAAGGTAGAGCTATCTCAACCAAATGCAGAACTCCGACTTCTTGAGGTTTTCTATCACAAGATCTACAAGATCTTCCCACACAGTGAAAAAATTGAGAATATAAATGACCAGTACTGGACATTGCGTGCAGAGGAGATTCCAGAAGAAGAGAAAAACTTGGGTCTTCATGACCGCTTGATTCACGTTTACCACTTTACAAAAGAGACTGCACAGAACCAGATGCAGGTACAGAATTTTGGGGAACCTTTCTTCTTGGTTATTCATGAAGGTGAAACTTTAGCTGAAGTGAAGGCACGCATACAAAAGAAATTGCAGGTCCCTGATGAAGAGTTCGCAAAGTGGAAGTTTGCATTTTTGTCATTGGGTCGTCCTGAGTACCTGCAGGACTCCGATGTAGTTTCCAGTCGTTTTCAGGTATCTTTGTGGTGTATTGTCATTTTGTATTTTCTGGTTTTCTTTGTAGAACAGCTGATGGTTAAATCCCAACTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004843 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009867 GO:0009987 GO:0010033 GO:0016787 GO:0019538 GO:0019783 GO:0023052 GO:0032870 GO:0036459 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070011 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0071704 GO:0101005 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04068 ko05168 ko05169 ko05203 map04068 map05168 map05169 map05203

Pfam Domains

Protein Families

MATH UCH USP7_C2 USP7_ICP0_bdg

Protein Analysis

1026

Amino Acids

120.24

Weight (kDa)

5.28

Isoelectric Point (pI)

47.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MATH_2	PF22486	53 - 122	1.3e-18	MATH domain
MATH	PF00917	58 - 121	4.5e-06	MATH domain
UCH	PF00443	160 - 445	2e-44	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	202 - 399	2.6e-08	Ubiquitin carboxyl-terminal hydrolase
USP7_ICP0_bdg	PF12436	549 - 800	2.2e-78	ICP0-binding domain of Ubiquitin-specific protease 7
USP7_C2	PF14533	810 - 999	3.4e-53	Ubiquitin-specific protease C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11910 AT3G11910 AT3G11910 AT3G11910 AT5G06600 AT5G06600 AT5G06600
fragaria_vesca	FvH4_3g26930 FvH4_3g39132 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220
malus_domestica	MD02G1261100.v1.1 MD03G1161100.v1.1 MD07G1059900.v1.1 MD11G1177100.v1.1 MD12G1047900.v1.1 MD12G1048000.v1.1 MD12G1048400.v1.1 MD12G1048500.v1.1 MD12G1048700.v1.1 MD12G1048800.v1.1 MD12G1056200.v1.1 MD14G1046300.v1.1 MD14G1046500.v1.1 MD15G1166900.v1.1
prunus_persica	Prupe.2G066200_v2.0.a1 Prupe.2G066200_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1
pyrus_communis	pycom02g22320 pycom03g11340 pycom07g04580 pycom11g14290 pycom11g15110 pycom12g04110 pycom12g04140 pycom12g04160 pycom12g04210 pycom12g04220 pycom12g04230 pycom12g04290 pycom12g04320 pycom12g04330 pycom12g04350 pycom12g04360 pycom14g03880 pycom14g03900 pycom16g18750
rosa_chinensis	RchiOBHm_Chr1g0324111 RchiOBHm_Chr1g0324241 RchiOBHm_Chr3g0477691 RchiOBHm_Chr5g0049371 RchiOBHm_Chr5g0070531
rosa_laevigata	RLG00000008612 RLG00000023698 RLG00000034613 RLG00000036139
rosa_multiflora	Rmu_co8271779.1_g000001 Rmu_sc0006187.1_g000014
rosa_roxburghii	Rroxscaffold_1G00010670 Rroxscaffold_1G00030450 Rroxscaffold_1G00031950 Rroxscaffold_1G00031970 Rroxscaffold_1G00032320 Rroxscaffold_1G00055490 Rroxscaffold_2G00083610 Rroxscaffold_2G00084480 Rroxscaffold_2G00115610 Rroxscaffold_3G00261320 Rroxscaffold_4G00312220 Rroxscaffold_4G00325820 Rroxscaffold_5G00339170 Rroxscaffold_5G00364030 Rroxscaffold_6G00403750
rosa_rugosa	Rorug03G0162700 Rorug03G0162800 Rorug03G0162900 Rorug05G0247700 Rorug05G0406600 Rorug05G0406800
rosa_samantha	Rh1AG060300 Rh3AG213000 Rh3BG246600 Rh3CG240400 Rh3DG239700 Rh5AG324400 Rh5AG462100 Rh5BG335500 Rh5BG480200 Rh5BG480300 Rh5CG360200 Rh5CG504500 Rh5CG504600 Rh5DG347800 Rh5DG492200 Rh5DG492300
rosa_wichuraiana	Rw3G019270 Rw3G026910 Rw5G030650 Rw5G032610 Rw5G043040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	237
AatII	GACGTC	1 cut(s)	2148
Acc16I	TGCGCA	2 cut(s)	345, 2325
Acc36I	ACCTGC	3 cut(s)	2800, 2890, 2973
AccB1I	GGYRCC	1 cut(s)	52
AccII	CGCG	1 cut(s)	1200
AciI	CCGC	5 cut(s)	56, 244, 305, 1081, 2758
AclWI	GGATC	2 cut(s)	1383, 2294
AcsI	RAATTY	4 cut(s)	158, 1010, 1812, 2818
AcuI	CTGAAG	5 cut(s)	455, 474, 923, 929, 2889
AcyI	GRCGYC	2 cut(s)	1586, 2145
AdeI	CACNNNGTG	1 cut(s)	2669
AfaI	GTAC	8 cut(s)	118, 688, 869, 1334, 2169, 2699, 2814, 2963
AfiI	CCNNNNNNNGG	3 cut(s)	1036, 1603, 2809
AflIII	ACRYGT	2 cut(s)	216, 1198
AhdI	GACNNNNNGTC	1 cut(s)	283
AjnI	CCWGG	2 cut(s)	1273, 1710
AjuI	GAANNNNNNNTTGG	2 cut(s)	1782, 1814
Alw21I	GWGCWC	3 cut(s)	423, 1776, 2555
Alw26I	GTCTC	2 cut(s)	395, 2783
AlwI	GGATC	2 cut(s)	1383, 2294
AoxI	GGCC	5 cut(s)	1157, 1461, 1662, 1802, 2470
ApoI	RAATTY	4 cut(s)	158, 1010, 1812, 2818
ArsI	GACNNNNNNTTYG	2 cut(s)	1517, 1549
AseI	ATTAAT	2 cut(s)	1956, 2571
AspLEI	GCGC	2 cut(s)	346, 2326
AspS9I	GGNCC	9 cut(s)	77, 1462, 1501, 1546, 1662, 1802, 2052, 2401, 2902
AsuC2I	CCSGG	1 cut(s)	1340
AsuHPI	GGTGA	5 cut(s)	869, 1050, 1259, 2533, 2867
AsuII	TTCGAA	1 cut(s)	1570
AvaII	GGWCC	6 cut(s)	77, 1501, 1546, 2052, 2401, 2902
AxyI	CCTNAGG	1 cut(s)	1400
BanI	GGYRCC	1 cut(s)	52
BanII	GRGCYC	2 cut(s)	423, 1776
BauI	CACGAG	1 cut(s)	1491
BbsI	GAAGAC	1 cut(s)	2741
Bbv12I	GWGCWC	3 cut(s)	423, 1776, 2555
BbvCI	CCTCAGC	1 cut(s)	81
BccI	CCATC	5 cut(s)	579, 1473, 2075, 2369, 3052
BciT130I	CCWGG	2 cut(s)	1275, 1712
BciVI	GTATCC	3 cut(s)	240, 583, 1623
BclI	TGATCA	1 cut(s)	1171
BcnI	CCSGG	1 cut(s)	1340
BcoDI	GTCTC	2 cut(s)	395, 2783
BfaI	CTAG	2 cut(s)	186, 1505
BfmI	CTRYAG	2 cut(s)	24, 2966
BfuAI	ACCTGC	3 cut(s)	2800, 2890, 2973
BfuI	GTATCC	3 cut(s)	240, 583, 1623
BglII	AGATCT	2 cut(s)	2646, 2655
BisI	GCNGC	1 cut(s)	56
BlsI	GCNGC	1 cut(s)	57
BmcAI	AGTACT	1 cut(s)	2699
Bme1390I	CCNGG	3 cut(s)	1275, 1340, 1712
Bme18I	GGWCC	6 cut(s)	77, 1501, 1546, 2052, 2401, 2902
BmeRI	GACNNNNNGTC	1 cut(s)	283
BmgT120I	GGNCC	9 cut(s)	77, 1462, 1501, 1546, 1662, 1802, 2052, 2401, 2902
BmiI	GGNNCC	6 cut(s)	54, 108, 144, 1085, 2829, 2904
BmrFI	CCNGG	3 cut(s)	1275, 1340, 1712
BmsI	GCATC	7 cut(s)	36, 507, 744, 886, 1248, 1757, 2796
BpiI	GAAGAC	1 cut(s)	2741
BpmI	CTGGAG	2 cut(s)	767, 915
Bpu10I	CCTNAGC	1 cut(s)	81
Bpu14I	TTCGAA	1 cut(s)	1570
BpuEI	CTTGAG	6 cut(s)	84, 419, 674, 872, 1760, 2648
BpuMI	CCSGG	1 cut(s)	1340
BsaBI	GATNNNNATC	1 cut(s)	1374
BsaHI	GRCGYC	2 cut(s)	1586, 2145
BsaJI	CCNNGG	3 cut(s)	87, 1710, 1839
Bsc4I	CCNNNNNNNGG	3 cut(s)	1036, 1603, 2809
Bse1I	ACTGG	6 cut(s)	134, 932, 1634, 2695, 2705, 2988
Bse21I	CCTNAGG	1 cut(s)	1400
Bse3DI	GCAATG	2 cut(s)	317, 2705
Bse8I	GATNNNNATC	1 cut(s)	1374
BseBI	CCWGG	2 cut(s)	1275, 1712
BseDI	CCNNGG	3 cut(s)	87, 1710, 1839
BseJI	GATNNNNATC	1 cut(s)	1374
BseLI	CCNNNNNNNGG	3 cut(s)	1036, 1603, 2809
BseMI	GCAATG	2 cut(s)	317, 2705
BseMII	CTCAG	5 cut(s)	95, 408, 465, 1869, 2949
BseNI	ACTGG	6 cut(s)	134, 932, 1634, 2695, 2705, 2988
BseRI	GAGGAG	6 cut(s)	6, 155, 1442, 1459, 2388, 2732
BsgI	GTGCAG	3 cut(s)	641, 2733, 2778
Bsh1236I	CGCG	1 cut(s)	1200
BshFI	GGCC	5 cut(s)	1159, 1463, 1664, 1804, 2472
BshNI	GGYRCC	1 cut(s)	52
BsiHKAI	GWGCWC	3 cut(s)	423, 1776, 2555
BsiSI	CCGG	2 cut(s)	1339, 1805
BslFI	GGGAC	7 cut(s)	992, 1355, 1538, 1674, 2099, 2132, 2888
BslI	CCNNNNNNNGG	3 cut(s)	1036, 1603, 2809
BsmAI	GTCTC	2 cut(s)	395, 2783
BsmFI	GGGAC	7 cut(s)	992, 1355, 1538, 1674, 2099, 2132, 2888
BsmI	GAATGC	1 cut(s)	737
BsnI	GGCC	5 cut(s)	1159, 1463, 1664, 1804, 2472
Bsp119I	TTCGAA	1 cut(s)	1570
Bsp1286I	GDGCHC	3 cut(s)	423, 1776, 2555
Bsp1407I	TGTACA	2 cut(s)	1332, 2167
Bsp143I	GATC	8 cut(s)	28, 778, 1000, 1171, 1375, 2299, 2646, 2655
Bsp19I	CCATGG	1 cut(s)	87
BspACI	CCGC	5 cut(s)	56, 244, 305, 1081, 2758
BspANI	GGCC	5 cut(s)	1159, 1463, 1664, 1804, 2472
BspCNI	CTCAG	5 cut(s)	94, 409, 466, 1870, 2950
BspFNI	CGCG	1 cut(s)	1200
BspHI	TCATGA	2 cut(s)	2752, 2848
BspLI	GGNNCC	6 cut(s)	54, 108, 144, 1085, 2829, 2904
BspMAI	CTGCAG	1 cut(s)	2970
BspMI	ACCTGC	3 cut(s)	2800, 2890, 2973
BspPI	GGATC	2 cut(s)	1383, 2294
BspQI	GCTCTTC	1 cut(s)	516
BspT104I	TTCGAA	1 cut(s)	1570
BspT107I	GGYRCC	1 cut(s)	52
BsrDI	GCAATG	2 cut(s)	317, 2705
BsrGI	TGTACA	2 cut(s)	1332, 2167
BsrI	ACTGG	6 cut(s)	134, 932, 1634, 2695, 2705, 2988
BssECI	CCNNGG	3 cut(s)	87, 1710, 1839
BssMI	GATC	8 cut(s)	28, 778, 1000, 1171, 1375, 2299, 2646, 2655
BssNI	GRCGYC	2 cut(s)	1586, 2145
BssSI	CACGAG	1 cut(s)	1491
BssT1I	CCWWGG	2 cut(s)	87, 1839
Bst2BI	CACGAG	1 cut(s)	1491
Bst2UI	CCWGG	2 cut(s)	1275, 1712
Bst6I	CTCTTC	8 cut(s)	36, 516, 1023, 1101, 1214, 1248, 2727, 2907
BstACI	GRCGYC	2 cut(s)	1586, 2145
BstAPI	GCANNNNNTGC	1 cut(s)	319
BstAUI	TGTACA	2 cut(s)	1332, 2167
BstBI	TTCGAA	1 cut(s)	1570
BstC8I	GCNNGC	5 cut(s)	111, 315, 1127, 2712, 2881
BstDEI	CTNAG	8 cut(s)	81, 417, 474, 900, 1108, 1400, 1878, 2958
BstDSI	CCRYGG	1 cut(s)	87
BstENI	CCTNNNNNAGG	1 cut(s)	1601
BstFNI	CGCG	1 cut(s)	1200
BstHHI	GCGC	2 cut(s)	346, 2326
BstKTI	GATC	8 cut(s)	31, 781, 1003, 1174, 1378, 2302, 2649, 2658
BstMAI	GTCTC	2 cut(s)	395, 2783
BstMBI	GATC	8 cut(s)	28, 778, 1000, 1171, 1375, 2299, 2646, 2655
BstMWI	GCNNNNNNNGC	5 cut(s)	55, 319, 1135, 1645, 1973
BstNI	CCWGG	2 cut(s)	1275, 1712
BstNSI	RCATGY	3 cut(s)	220, 487, 1327
BstSCI	CCNGG	3 cut(s)	1273, 1338, 1710
BstSFI	CTRYAG	2 cut(s)	24, 2966
BstUI	CGCG	1 cut(s)	1200
BstV2I	GAAGAC	1 cut(s)	2741
BstX2I	RGATCY	2 cut(s)	2646, 2655
BstYI	RGATCY	2 cut(s)	2646, 2655
Bsu36I	CCTNAGG	1 cut(s)	1400
BsuI	GTATCC	3 cut(s)	240, 583, 1623
BsuRI	GGCC	5 cut(s)	1159, 1463, 1664, 1804, 2472
BtgI	CCRYGG	1 cut(s)	87
BtsI	GCAGTG	2 cut(s)	556, 2319
BtsIMutI	CAGTG	8 cut(s)	141, 556, 1181, 1351, 1641, 2319, 2553, 2674
BveI	ACCTGC	3 cut(s)	2800, 2890, 2973
Cac8I	GCNNGC	5 cut(s)	111, 315, 1127, 2712, 2881
CciI	TCATGA	2 cut(s)	2752, 2848
CfoI	GCGC	2 cut(s)	346, 2326
Cfr13I	GGNCC	9 cut(s)	77, 1462, 1501, 1546, 1662, 1802, 2052, 2401, 2902
CseI	GACGC	1 cut(s)	1594
Csp6I	GTAC	8 cut(s)	117, 687, 868, 1333, 2168, 2698, 2813, 2962
CviQI	GTAC	8 cut(s)	117, 687, 868, 1333, 2168, 2698, 2813, 2962
DdeI	CTNAG	8 cut(s)	81, 417, 474, 900, 1108, 1400, 1878, 2958
DpnI	GATC	8 cut(s)	30, 780, 1002, 1173, 1377, 2301, 2648, 2657
DpnII	GATC	8 cut(s)	28, 778, 1000, 1171, 1375, 2299, 2646, 2655
DraIII	CACNNNGTG	1 cut(s)	2669
DriI	GACNNNNNGTC	1 cut(s)	283
Eam1104I	CTCTTC	8 cut(s)	36, 516, 1023, 1101, 1214, 1248, 2727, 2907
Eam1105I	GACNNNNNGTC	1 cut(s)	283
EarI	CTCTTC	8 cut(s)	36, 516, 1023, 1101, 1214, 1248, 2727, 2907
EciI	GGCGGA	2 cut(s)	259, 320
Ecl136II	GAGCTC	2 cut(s)	421, 1774
Eco130I	CCWWGG	2 cut(s)	87, 1839
Eco147I	AGGCCT	1 cut(s)	2472
Eco24I	GRGCYC	2 cut(s)	423, 1776
Eco47I	GGWCC	6 cut(s)	77, 1501, 1546, 2052, 2401, 2902
Eco53kI	GAGCTC	2 cut(s)	421, 1774
Eco57I	CTGAAG	5 cut(s)	455, 474, 923, 929, 2889
Eco81I	CCTNAGG	1 cut(s)	1400
EcoICRI	GAGCTC	2 cut(s)	421, 1774
EcoNI	CCTNNNNNAGG	1 cut(s)	1601
EcoO109I	RGGNCCY	4 cut(s)	77, 1501, 2052, 2902
EcoRII	CCWGG	2 cut(s)	1273, 1710
EcoT14I	CCWWGG	2 cut(s)	87, 1839
EcoT22I	ATGCAT	1 cut(s)	737
EcoT38I	GRGCYC	2 cut(s)	423, 1776
ErhI	CCWWGG	2 cut(s)	87, 1839
FalI	AAGNNNNNCTT	6 cut(s)	366, 398, 487, 519, 1782, 1814
FaqI	GGGAC	7 cut(s)	992, 1355, 1538, 1674, 2099, 2132, 2888
FauNDI	CATATG	2 cut(s)	462, 595
FbaI	TGATCA	1 cut(s)	1171
Fnu4HI	GCNGC	1 cut(s)	56
FriOI	GRGCYC	2 cut(s)	423, 1776
Fsp4HI	GCNGC	1 cut(s)	56
FspBI	CTAG	2 cut(s)	186, 1505
FspI	TGCGCA	2 cut(s)	345, 2325
GlaI	GCGC	2 cut(s)	345, 2325
GluI	GCNGC	1 cut(s)	56
GsuI	CTGGAG	2 cut(s)	767, 915
HaeIII	GGCC	5 cut(s)	1159, 1463, 1664, 1804, 2472
HapII	CCGG	2 cut(s)	1339, 1805
HgaI	GACGC	1 cut(s)	1594
HhaI	GCGC	2 cut(s)	346, 2326
Hin1I	GRCGYC	2 cut(s)	1586, 2145
Hin6I	GCGC	2 cut(s)	344, 2324
HinP1I	GCGC	2 cut(s)	344, 2324
HindIII	AAGCTT	2 cut(s)	530, 650
HpaII	CCGG	2 cut(s)	1339, 1805
HphI	GGTGA	5 cut(s)	869, 1050, 1259, 2533, 2867
Hpy99I	CGWCG	1 cut(s)	230
HpyCH4IV	ACGT	3 cut(s)	847, 2145, 2769
HpyF10VI	GCNNNNNNNGC	5 cut(s)	55, 319, 1135, 1645, 1973
HpyF3I	CTNAG	8 cut(s)	81, 417, 474, 900, 1108, 1400, 1878, 2958
HpySE526I	ACGT	3 cut(s)	847, 2145, 2769
Hsp92I	GRCGYC	2 cut(s)	1586, 2145
HspAI	GCGC	2 cut(s)	344, 2324
Ksp22I	TGATCA	1 cut(s)	1171
Kzo9I	GATC	8 cut(s)	28, 778, 1000, 1171, 1375, 2299, 2646, 2655
LguI	GCTCTTC	1 cut(s)	516
LmnI	GCTCC	3 cut(s)	106, 142, 1981
LweI	GCATC	7 cut(s)	36, 507, 744, 886, 1248, 1757, 2796
MaeI	CTAG	2 cut(s)	186, 1505
MaeII	ACGT	3 cut(s)	847, 2145, 2769
MaeIII	GTNAC	6 cut(s)	376, 412, 857, 1056, 1201, 1346
MalI	GATC	8 cut(s)	30, 780, 1002, 1173, 1377, 2301, 2648, 2657
MboI	GATC	8 cut(s)	28, 778, 1000, 1171, 1375, 2299, 2646, 2655
MfeI	CAATTG	2 cut(s)	171, 1736
MflI	RGATCY	2 cut(s)	2646, 2655
MhlI	GDGCHC	3 cut(s)	423, 1776, 2555
MluI	ACGCGT	1 cut(s)	1198
MlyI	GAGTC	4 cut(s)	7, 776, 2310, 2966
MmeI	TCCRAC	4 cut(s)	173, 311, 1711, 2644
Mph1103I	ATGCAT	1 cut(s)	737
MslI	CAYNNNNRTG	6 cut(s)	740, 1119, 1143, 1328, 2253, 2853
MspA1I	CMGCKG	1 cut(s)	3055
MspI	CCGG	2 cut(s)	1339, 1805
MspR9I	CCNGG	3 cut(s)	1275, 1340, 1712
MunI	CAATTG	2 cut(s)	171, 1736
Mva1269I	GAATGC	1 cut(s)	737
MvaI	CCWGG	2 cut(s)	1275, 1712
MvnI	CGCG	1 cut(s)	1200
MwoI	GCNNNNNNNGC	5 cut(s)	55, 319, 1135, 1645, 1973
NciI	CCSGG	1 cut(s)	1340
NcoI	CCATGG	1 cut(s)	87
NdeI	CATATG	2 cut(s)	462, 595
NdeII	GATC	8 cut(s)	28, 778, 1000, 1171, 1375, 2299, 2646, 2655
NlaIV	GGNNCC	6 cut(s)	54, 108, 144, 1085, 2829, 2904
NmuCI	GTSAC	4 cut(s)	857, 1056, 1201, 1346
NsbI	TGCGCA	2 cut(s)	345, 2325
NsiI	ATGCAT	1 cut(s)	737
NspI	RCATGY	3 cut(s)	220, 487, 1327
NspV	TTCGAA	1 cut(s)	1570
PagI	TCATGA	2 cut(s)	2752, 2848
PceI	AGGCCT	1 cut(s)	2472
PciI	ACATGT	1 cut(s)	216
PciSI	GCTCTTC	1 cut(s)	516
PcsI	WCGNNNNNNNCGW	1 cut(s)	1693
PctI	GAATGC	1 cut(s)	737
PfeI	GAWTC	8 cut(s)	308, 599, 1064, 1572, 1650, 2524, 2722, 2764
PflFI	GACNNNGTC	1 cut(s)	1556
PfoI	TCCNGGA	1 cut(s)	1338
PkrI	GCNGC	1 cut(s)	57
PleI	GAGTC	4 cut(s)	7, 775, 2310, 2966
PpsI	GAGTC	4 cut(s)	7, 775, 2310, 2966
PpuMI	RGGWCCY	4 cut(s)	77, 1501, 2052, 2902
PscI	ACATGT	1 cut(s)	216
PshBI	ATTAAT	2 cut(s)	1956, 2571
PsiI	TTATAA	1 cut(s)	237
Psp124BI	GAGCTC	2 cut(s)	423, 1776
Psp5II	RGGWCCY	4 cut(s)	77, 1501, 2052, 2902
Psp6I	CCWGG	2 cut(s)	1273, 1710
PspGI	CCWGG	2 cut(s)	1273, 1710
PspN4I	GGNNCC	6 cut(s)	54, 108, 144, 1085, 2829, 2904
PspPI	GGNCC	9 cut(s)	77, 1462, 1501, 1546, 1662, 1802, 2052, 2401, 2902
PspPPI	RGGWCCY	4 cut(s)	77, 1501, 2052, 2902
PstI	CTGCAG	1 cut(s)	2970
PsuI	RGATCY	2 cut(s)	2646, 2655
PsyI	GACNNNGTC	1 cut(s)	1556
PvuII	CAGCTG	1 cut(s)	3055
RsaI	GTAC	8 cut(s)	118, 688, 869, 1334, 2169, 2699, 2814, 2963
RsaNI	GTAC	8 cut(s)	117, 687, 868, 1333, 2168, 2698, 2813, 2962
RseI	CAYNNNNRTG	6 cut(s)	740, 1119, 1143, 1328, 2253, 2853
SacI	GAGCTC	2 cut(s)	423, 1776
SapI	GCTCTTC	1 cut(s)	516
SatI	GCNGC	1 cut(s)	56
Sau3AI	GATC	8 cut(s)	28, 778, 1000, 1171, 1375, 2299, 2646, 2655
Sau96I	GGNCC	9 cut(s)	77, 1462, 1501, 1546, 1662, 1802, 2052, 2401, 2902
SbfI	CCTGCAGG	1 cut(s)	2970
ScaI	AGTACT	1 cut(s)	2699
SchI	GAGTC	4 cut(s)	7, 776, 2310, 2966
ScrFI	CCNGG	3 cut(s)	1275, 1340, 1712
SdaI	CCTGCAGG	1 cut(s)	2970
SduI	GDGCHC	3 cut(s)	423, 1776, 2555
SfaNI	GCATC	7 cut(s)	36, 507, 744, 886, 1248, 1757, 2796
SfcI	CTRYAG	2 cut(s)	24, 2966
SfuI	TTCGAA	1 cut(s)	1570
SinI	GGWCC	6 cut(s)	77, 1501, 1546, 2052, 2401, 2902
SmiMI	CAYNNNNRTG	6 cut(s)	740, 1119, 1143, 1328, 2253, 2853
SmlI	CTYRAG	6 cut(s)	99, 398, 653, 851, 1775, 2627
SmoI	CTYRAG	6 cut(s)	99, 398, 653, 851, 1775, 2627
Sse8387I	CCTGCAGG	1 cut(s)	2970
SseBI	AGGCCT	1 cut(s)	2472
SsiI	CCGC	5 cut(s)	56, 244, 305, 1081, 2758
SspMI	CTAG	2 cut(s)	186, 1505
SstI	GAGCTC	2 cut(s)	423, 1776
StuI	AGGCCT	1 cut(s)	2472
StyD4I	CCNGG	3 cut(s)	1273, 1338, 1710
StyI	CCWWGG	2 cut(s)	87, 1839
TaiI	ACGT	3 cut(s)	850, 2148, 2772
TaqI	TCGA	5 cut(s)	72, 1313, 1570, 2062, 2371
TatI	WGTACW	4 cut(s)	116, 1332, 2167, 2697
TauI	GCSGC	1 cut(s)	58
TfiI	GAWTC	8 cut(s)	308, 599, 1064, 1572, 1650, 2524, 2722, 2764
TscAI	CASTG	8 cut(s)	141, 556, 1181, 1351, 1641, 2326, 2560, 2674
TseFI	GTSAC	4 cut(s)	857, 1056, 1201, 1346
Tsp45I	GTSAC	4 cut(s)	857, 1056, 1201, 1346
TspGWI	ACGGA	2 cut(s)	77, 1550
TspRI	CASTG	8 cut(s)	141, 556, 1181, 1351, 1641, 2326, 2560, 2674
Tth111I	GACNNNGTC	1 cut(s)	1556
VpaK11BI	GGWCC	6 cut(s)	77, 1501, 1546, 2052, 2401, 2902
VspI	ATTAAT	2 cut(s)	1956, 2571
XagI	CCTNNNNNAGG	1 cut(s)	1601
XapI	RAATTY	4 cut(s)	158, 1010, 1812, 2818
XceI	RCATGY	3 cut(s)	220, 487, 1327
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1271
XspI	CTAG	2 cut(s)	186, 1505
ZraI	GACGTC	1 cut(s)	2146
ZrmI	AGTACT	1 cut(s)	2699
Zsp2I	ATGCAT	1 cut(s)	737

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.