Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0000333

Physical Location & Seq

Genomic Coordinates Forward (+)

5555290 .. 5585978

Gene Structure 30689 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1059900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3363 bp

ATGACTCTGATGACTCCGCCGCCGCTAGATCAGCAGCAGCAGCAGCAACCGCAGGAGGAAGATGAGATGCTGGTCCCACACTCCGACCTCCCCGACGGTCCTCAGCCCATGGAGGAAGCACAAGCAGAAACTAACAATGCAGTGGATGCTCCGACGGTGGATGATCCATTGTCAGCTAGATTTACATGGCCAATTGAGAGCTTTTCAAGGCTTAATACGAAGAAGCTCTATTCTGATGTTTTCCTTGTAGGAGGCTACAAATGGCGGATACTAATTTTCCCCAAAGGAAATAATGTGGATCATCTGTCAATGTATCTGGATGTTGCAGATTCTGGAAATTTGCCTTATGGATGGAGTAGATATGCTCAGTTTAGCTTGTCTATTGTCAATCAAGTTAATAGTAAATACTCTATTAGAAAAGAAACACAGCACCAATTTAATGCTCGTGAGAGTGACTGGGGTTTCACTTCGTTCATGCCTCTTGGTGAATTATATGACCCTAGTAGAGGTTATATTGTGAATGATAGATGCATAGTTGAAGCTGATGTTGCAGTTCGAAAGGTTATTGATTACTGGTCACATGACTCTAAAAAGGAAACTGGTTTTGTTGGACTGAAAAACCAAGGAGCTACTTGTTATATGAACTCTCTTCTTCAGACGCTGTACCATATTCCTTATTTTAGAAAGGCTGTGTACCATATGCCAACAACGGAGAATGATAATCCATCTGGAAGCATCCCACTTGCCTTGCAAAGCTTATTTTACAAGCTTCAGTACAATGATACTAGTGTAGCGACCAAAGAGCTGACGAAGTCATTTGGATGGGATGCATATGATTCATTCATGCAGCATGACGTCCAAGAACTCAATAGGGTTCTTTCTGAAAAGCTTGAAGACAAGATGAAAGGAACTGTTGTGGAGGGTACAATACAGCAGTTATTTGAAGGGCACCAGATGAACTATATTGAATGCATCAATGTGGACTATAAATCAACGAGGAAAGAATCATTTTATGATCTTCAATTGGATGTCAAAGGGTGTCGGGATGTTTATGCTTCATTCGATAAGTATGTGGAAGTGGAGCGCCTTGAGGGTGATAATAAATATCATGCTGAACAGTATGGTTTACAGGATGCTAAGAAAGGTGTCCTGTTCATTGATTTTCCACCTGTCCTTCAACTTCAGCTAAAACGTTTCGAGTACGATTTTATGCGGGACACCATGGTAAAGATAAATGATCGCTATGAGTTCCCCTTGCAACTTGATCTTGATAGGGAGAATGGGAAATATCTGTCGCCTGATGCTGACAGGAGCGTCCGCAACCTCTACACTCTTCATAGTGTTTTGGTGCACAGTGGCGGGGTGCATGGTGGGCACTACTATGCTTATATCAGGCCAACCCTTTCTGATCAGTGGTTTAAATTTGATGATGAACGAGTAACAAAAGAAGATATGAAGAGGGCTCTAGAGGAGCAGTATGGTGGAGAAGAAGAGTTACCGCAGACAAATCCTGGGTTCAACAATGCTCCATTTAAATTTACTAAATACTCCAATGCCTACATGCTTGTTTATATACGTGAAGTTGACAAGGAGAAAATAATTTGTAATGTGGATGAGAAAGACATTGCAGAACATCTAAGGATAAGACTGAAGAAAGAACAGGAAGAAAAGGAGCAAAAGAGAAAGGAGAAAGCTGAAGCGCACCTTTATACTATCATAAAGGTTGCGCGGAACGAGGACCTGCTTGAACAGATAGGAAAGAATATCTATTTTGATCTTGTGGATCATGATAAAGTTCGTAGTTTTCGTATTCAGAAGCAGATGCCCTTCAACCTCTTTAAGGAAGAGGTTGCCAAAGAGTTTGGTATACCAGTTCAATGTCAACGTTTCTGGTTGTGGGCAAAGCGTCAAAACCATACATACCGGCCAAATCGACCACTGACACCTCTTGAGGAAGCACAATCTGTTGGACAGTTGAGGGAAGTTTCCAATAAGTCAAATAATGCAGAGCTAAAGCTGTTTCTTGAAGTAGAACTTGGGCCGGATTTGTTGCCTCTTTCTCCTGCTGAGAAGACTAAAGAAGAGATTCTTCTATTTTTCAAACTTTATGACCCTTTGAAAGAAGAGCTCCGGTACGTTGGGAGGTTGTTTGTGAAGGGAAGTGGTAAGCCCATTGAATTATTGACAAAACTAAACCAAATGGCTGGTTTCAGTCCTGATGAAAAGATAGAATTTTTTGAGGAAATAAAGTTTGAACCTAATATCATGTGTGAACACATTGATGAGAAGGCAACTTTCCGTGTTAGTCAGCTTGAAGATGGGGACATCATTTGCTTTCAAAAATCCCCTCAGAGTGGAAGCAGCGAACAATTCCGTTACCCAGATGTTCCTTCATTCCTGGATTATGTTCGTAACCGTCAGGTTGTTCGCTTTCGTTCTTTGGACAAGCCAAAGGAAGATGAGTTCTGTCTTGAGTTGTCAAAGTTTCACACCTATGATGATGTTGTGGAAAGGGTTGCTCAACGGCTTAGCTTGGATGATCCATCTAAAGTTAGGCTCACATCTCATAATTGTTACTCACAGCAACCTAAACCCCAACCAATCAAGTATCGAGGAGTGGAACATTTGTCTGACATGCTGGTGCACTACAATCAGACTACGGATGTACTCTATTATGAAGTATTAGATATCCCTCTCCCAGAGCTGCAAGGCCTCAAAACTTTGAAAGTTGCTTTTCATCATGCTACCAAGGATGAAGTTGTGGTTCATACTATTAGATTACCGAAGCAGAGCACTGTTGGGGATGTGATTGATGACCTTAAGACAAAGGTGGAGTTGTCTCATCCTAATGCCGAACTCAGATTGTTAGAAGTTTTCTATCACAAAATCTACAAGATTTTCCCGCCAAATGAGAAGATTGAGAATATAAATGATCAGTACTGGACATTGCGTGCAGAAGAGATCCCTGAAGAAGAGAAAAACTTTGGCCCTCGTGATCGTCTGATCCATGTTTATCATTTTATGAAGGACACAGCTCAAAACCAGGTTCAGAATTTCGGAGAACCATTTTTCTTGGTCATTCGTGAGGATGAGACATTAGCAGAAGTTAAAGTGCGTGTACAGAAAAAGTTACAAGTCCCAGATGAGGAGTATTCAAAGTGGAAATTTGCATTTTTGTCCATGGGTCGTCCTGAGTACCTGCAAGACGATGATATTGTGGCCAGCCGATTTCAGAGAAGGGATGTGTATGGAGCATGGGAACAGTACCTTGGATTGGAACACGCTGATCATGCTCCTAAAAGGTCACACACAACAAATCAGAATCGTCACACCTTTGAGAAGCCAGTGAAAATCTACAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004843 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009867 GO:0009987 GO:0010033 GO:0016787 GO:0019538 GO:0019783 GO:0023052 GO:0032870 GO:0036459 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070011 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0071704 GO:0101005 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04068 ko05168 ko05169 ko05203 map04068 map05168 map05169 map05203

Pfam Domains

Protein Families

MATH UCH USP7_C2 USP7_ICP0_bdg

Protein Analysis

1121

Amino Acids

131.37

Weight (kDa)

5.57

Isoelectric Point (pI)

42.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MATH_2	PF22486	60 - 183	5.8e-39	MATH domain
MATH	PF00917	65 - 185	1.6e-16	MATH domain
UCH	PF00443	203 - 524	6.6e-60	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	203 - 478	2e-14	Ubiquitin carboxyl-terminal hydrolase
USP7_ICP0_bdg	PF12436	628 - 880	3.3e-76	ICP0-binding domain of Ubiquitin-specific protease 7
USP7_C2	PF14533	890 - 1098	4.1e-57	Ubiquitin-specific protease C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0000333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11910 AT3G11910 AT3G11910 AT3G11910 AT5G06600 AT5G06600 AT5G06600
fragaria_vesca	FvH4_3g26930 FvH4_3g39132 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220
malus_domestica	MD02G1261100.v1.1 MD03G1161100.v1.1 MD07G1059900.v1.1 MD11G1177100.v1.1 MD12G1047900.v1.1 MD12G1048000.v1.1 MD12G1048400.v1.1 MD12G1048500.v1.1 MD12G1048700.v1.1 MD12G1048800.v1.1 MD12G1056200.v1.1 MD14G1046300.v1.1 MD14G1046500.v1.1 MD15G1166900.v1.1
prunus_persica	Prupe.2G066200_v2.0.a1 Prupe.2G066200_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1
pyrus_communis	pycom02g22320 pycom03g11340 pycom07g04580 pycom11g14290 pycom11g15110 pycom12g04110 pycom12g04140 pycom12g04160 pycom12g04210 pycom12g04220 pycom12g04230 pycom12g04290 pycom12g04320 pycom12g04330 pycom12g04350 pycom12g04360 pycom14g03880 pycom14g03900 pycom16g18750
rosa_chinensis	RchiOBHm_Chr1g0324111 RchiOBHm_Chr1g0324241 RchiOBHm_Chr3g0477691 RchiOBHm_Chr5g0049371 RchiOBHm_Chr5g0070531
rosa_laevigata	RLG00000008612 RLG00000023698 RLG00000034613 RLG00000036139
rosa_multiflora	Rmu_co8271779.1_g000001 Rmu_sc0006187.1_g000014
rosa_roxburghii	Rroxscaffold_1G00010670 Rroxscaffold_1G00030450 Rroxscaffold_1G00031950 Rroxscaffold_1G00031970 Rroxscaffold_1G00032320 Rroxscaffold_1G00055490 Rroxscaffold_2G00083610 Rroxscaffold_2G00084480 Rroxscaffold_2G00115610 Rroxscaffold_3G00261320 Rroxscaffold_4G00312220 Rroxscaffold_4G00325820 Rroxscaffold_5G00339170 Rroxscaffold_5G00364030 Rroxscaffold_6G00403750
rosa_rugosa	Rorug03G0162700 Rorug03G0162800 Rorug03G0162900 Rorug05G0247700 Rorug05G0406600 Rorug05G0406800
rosa_samantha	Rh1AG060300 Rh3AG213000 Rh3BG246600 Rh3CG240400 Rh3DG239700 Rh5AG324400 Rh5AG462100 Rh5BG335500 Rh5BG480200 Rh5BG480300 Rh5CG360200 Rh5CG504500 Rh5CG504600 Rh5DG347800 Rh5DG492200 Rh5DG492300
rosa_wichuraiana	Rw3G019270 Rw3G026910 Rw5G030650 Rw5G032610 Rw5G043040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1313
AatII	GACGTC	1 cut(s)	858
Acc36I	ACCTGC	2 cut(s)	1749, 3207
AccB1I	GGYRCC	1 cut(s)	948
AccI	GTMKAC	1 cut(s)	1867
AccII	CGCG	1 cut(s)	1729
AclI	AACGTT	2 cut(s)	1192, 1885
AclWI	GGATC	6 cut(s)	158, 306, 1791, 2534, 2956, 2998
AcoI	YGGCCR	3 cut(s)	188, 1925, 3219
AcsI	RAATTY	6 cut(s)	337, 1421, 1535, 2231, 3052, 3164
AcuI	CTGAAG	6 cut(s)	638, 755, 1166, 1670, 1716, 2988
AcyI	GRCGYC	1 cut(s)	855
AfiI	CCNNNNNNNGG	5 cut(s)	506, 1840, 2354, 3145, 3274
AflII	CTTAAG	1 cut(s)	2819
AhlI	ACTAGT	1 cut(s)	785
AjnI	CCWGG	3 cut(s)	1510, 2397, 3042
AjuI	GAANNNNNNNTTGG	4 cut(s)	2019, 2051, 3252, 3284
AloI	GAACNNNNNNTCC	2 cut(s)	2447, 2479
Alw21I	GWGCWC	4 cut(s)	1353, 2130, 2646, 2795
Alw26I	GTCTC	2 cut(s)	2844, 3086
Alw44I	GTGCAC	2 cut(s)	1349, 2642
AlwI	GGATC	6 cut(s)	158, 306, 1791, 2534, 2956, 2998
AlwNI	CAGNNNCTG	2 cut(s)	332, 661
AoxI	GGCC	7 cut(s)	188, 1394, 1925, 2039, 2710, 2986, 3219
ApaLI	GTGCAC	2 cut(s)	1349, 2642
ApeKI	GCWGC	7 cut(s)	34, 37, 40, 43, 847, 2361, 2704
ApoI	RAATTY	6 cut(s)	337, 1421, 1535, 2231, 3052, 3164
Asp700I	GAANNNNTTC	1 cut(s)	2085
AspLEI	GCGC	3 cut(s)	1086, 1702, 1729
AspS9I	GGNCC	5 cut(s)	73, 98, 1738, 2039, 2987
AsuHPI	GGTGA	2 cut(s)	497, 1106
AsuII	TTCGAA	1 cut(s)	556
AvaII	GGWCC	3 cut(s)	73, 98, 1738
BaeGI	GKGCMC	4 cut(s)	951, 1353, 1377, 2646
BaeI	ACNNNNGTAYC	2 cut(s)	774, 807
BalI	TGGCCA	2 cut(s)	190, 3221
BanI	GGYRCC	1 cut(s)	948
BanII	GRGCYC	2 cut(s)	1465, 2130
BarI	GAAGNNNNNNTAC	2 cut(s)	1479, 1511
BauI	CACGAG	2 cut(s)	444, 2991
BbsI	GAAGAC	2 cut(s)	900, 2078
Bbv12I	GWGCWC	4 cut(s)	1353, 2130, 2646, 2795
BbvCI	CCTCAGC	1 cut(s)	102
BbvI	GCAGC	7 cut(s)	46, 49, 52, 55, 859, 2373, 2691
BccI	CCATC	5 cut(s)	345, 733, 816, 2312, 2551
BceAI	ACGGC	1 cut(s)	2540
BciT130I	CCWGG	3 cut(s)	1512, 2399, 3044
BciVI	GTATCC	1 cut(s)	261
BclI	TGATCA	3 cut(s)	1408, 2932, 3286
BcoDI	GTCTC	2 cut(s)	2844, 3086
BcuI	ACTAGT	1 cut(s)	785
BfaI	CTAG	5 cut(s)	26, 177, 501, 786, 1466
BfoI	RGCGCY	1 cut(s)	1087
BfrI	CTTAAG	1 cut(s)	2819
BfuAI	ACCTGC	2 cut(s)	1749, 3207
BfuI	GTATCC	1 cut(s)	261
BisI	GCNGC	9 cut(s)	20, 23, 35, 38, 41, 44, 848, 2362, 2705
BlpI	GCTNAGC	1 cut(s)	2528
BlsI	GCNGC	9 cut(s)	21, 24, 36, 39, 42, 45, 849, 2363, 2706
BmcAI	AGTACT	1 cut(s)	2939
Bme1390I	CCNGG	3 cut(s)	1512, 2399, 3044
Bme18I	GGWCC	3 cut(s)	73, 98, 1738
BmgT120I	GGNCC	5 cut(s)	73, 98, 1738, 2039, 2987
BmiI	GGNNCC	2 cut(s)	75, 950
BmrFI	CCNGG	3 cut(s)	1512, 2399, 3044
BmrI	ACTGGG	1 cut(s)	466
BmsI	GCATC	9 cut(s)	57, 136, 518, 744, 817, 981, 1123, 1291, 1812
BmuI	ACTGGG	1 cut(s)	466
BpiI	GAAGAC	2 cut(s)	900, 2078
Bpu10I	CCTNAGC	1 cut(s)	102
Bpu1102I	GCTNAGC	1 cut(s)	2528
Bpu14I	TTCGAA	1 cut(s)	556
BpuEI	CTTGAG	3 cut(s)	1109, 1970, 2492
BsaAI	YACGTR	1 cut(s)	1577
BsaHI	GRCGYC	1 cut(s)	855
BsaJI	CCNNGG	7 cut(s)	108, 622, 1221, 1511, 2748, 3180, 3268
BsaWI	WCCGGW	1 cut(s)	2130
Bsc4I	CCNNNNNNNGG	5 cut(s)	506, 1840, 2354, 3145, 3274
Bse118I	RCCGGY	1 cut(s)	1923
Bse1I	ACTGG	6 cut(s)	461, 578, 604, 1871, 2945, 3344
Bse3DI	GCAATG	2 cut(s)	1623, 2945
BseBI	CCWGG	3 cut(s)	1512, 2399, 3044
BseDI	CCNNGG	7 cut(s)	108, 622, 1221, 1511, 2748, 3180, 3268
BseLI	CCNNNNNNNGG	5 cut(s)	506, 1840, 2354, 3145, 3274
BseMI	GCAATG	2 cut(s)	1623, 2945
BseMII	CTCAG	6 cut(s)	116, 380, 2058, 2363, 2872, 3183
BseNI	ACTGG	6 cut(s)	461, 578, 604, 1871, 2945, 3344
BseRI	GAGGAG	3 cut(s)	1484, 2628, 3161
BseSI	GKGCMC	4 cut(s)	951, 1353, 1377, 2646
BseXI	GCAGC	7 cut(s)	46, 49, 52, 55, 859, 2373, 2691
BsgI	GTGCAG	1 cut(s)	2973
Bsh1236I	CGCG	1 cut(s)	1729
BshFI	GGCC	7 cut(s)	190, 1396, 1927, 2041, 2712, 2988, 3221
BshNI	GGYRCC	1 cut(s)	948
BsiHKAI	GWGCWC	4 cut(s)	1353, 2130, 2646, 2795
BsiSI	CCGG	3 cut(s)	1924, 2042, 2131
BslFI	GGGAC	4 cut(s)	59, 1229, 2336, 3122
BslI	CCNNNNNNNGG	5 cut(s)	506, 1840, 2354, 3145, 3274
BsmAI	GTCTC	2 cut(s)	2844, 3086
BsmFI	GGGAC	4 cut(s)	59, 1229, 2336, 3122
BsmI	GAATGC	1 cut(s)	974
BsnI	GGCC	7 cut(s)	190, 1396, 1927, 2041, 2712, 2988, 3221
Bsp119I	TTCGAA	1 cut(s)	556
Bsp1286I	GDGCHC	7 cut(s)	951, 1353, 1377, 1465, 2130, 2646, 2795
Bsp1407I	TGTACA	1 cut(s)	3118
Bsp1720I	GCTNAGC	1 cut(s)	2528
Bsp19I	CCATGG	3 cut(s)	108, 1221, 3180
BspANI	GGCC	7 cut(s)	190, 1396, 1927, 2041, 2712, 2988, 3221
BspCNI	CTCAG	6 cut(s)	115, 379, 2059, 2362, 2871, 3184
BspFNI	CGCG	1 cut(s)	1729
BspHI	TCATGA	1 cut(s)	1786
BspLI	GGNNCC	2 cut(s)	75, 950
BspMI	ACCTGC	2 cut(s)	1749, 3207
BspPI	GGATC	6 cut(s)	158, 306, 1791, 2534, 2956, 2998
BspQI	GCTCTTC	1 cut(s)	2118
BspT104I	TTCGAA	1 cut(s)	556
BspT107I	GGYRCC	1 cut(s)	948
BspTI	CTTAAG	1 cut(s)	2819
BsrDI	GCAATG	2 cut(s)	1623, 2945
BsrFI	RCCGGY	1 cut(s)	1923
BsrGI	TGTACA	1 cut(s)	3118
BsrI	ACTGG	6 cut(s)	461, 578, 604, 1871, 2945, 3344
BssAI	RCCGGY	1 cut(s)	1923
BssECI	CCNNGG	7 cut(s)	108, 622, 1221, 1511, 2748, 3180, 3268
BssNAI	GTATAC	1 cut(s)	1868
BssNI	GRCGYC	1 cut(s)	855
BssSI	CACGAG	2 cut(s)	444, 2991
BssT1I	CCWWGG	6 cut(s)	108, 622, 1221, 2748, 3180, 3268
Bst1107I	GTATAC	1 cut(s)	1868
Bst2BI	CACGAG	2 cut(s)	444, 2991
Bst2UI	CCWGG	3 cut(s)	1512, 2399, 3044
Bst4CI	ACNGT	9 cut(s)	98, 157, 913, 1119, 1355, 1974, 2417, 2797, 3264
Bst6I	CTCTTC	9 cut(s)	654, 1338, 1451, 1485, 1839, 2076, 2118, 2952, 2967
BstACI	GRCGYC	1 cut(s)	855
BstAFI	CTTAAG	1 cut(s)	2819
BstAPI	GCANNNNNTGC	1 cut(s)	146
BstAUI	TGTACA	1 cut(s)	3118
BstBAI	YACGTR	1 cut(s)	1577
BstBI	TTCGAA	1 cut(s)	556
BstC8I	GCNNGC	2 cut(s)	2952, 3223
BstDEI	CTNAG	9 cut(s)	102, 366, 1137, 1637, 2067, 2349, 2528, 2858, 3192
BstDSI	CCRYGG	3 cut(s)	108, 1221, 3180
BstENI	CCTNNNNNAGG	2 cut(s)	504, 1838
BstFNI	CGCG	1 cut(s)	1729
BstH2I	RGCGCY	1 cut(s)	1087
BstHHI	GCGC	3 cut(s)	1086, 1702, 1729
BstMAI	GTCTC	2 cut(s)	2844, 3086
BstMWI	GCNNNNNNNGC	8 cut(s)	31, 40, 43, 49, 146, 548, 1372, 3290
BstNI	CCWGG	3 cut(s)	1512, 2399, 3044
BstNSI	RCATGY	2 cut(s)	1564, 2638
BstSCI	CCNGG	3 cut(s)	1510, 2397, 3042
BstSLI	GKGCMC	4 cut(s)	951, 1353, 1377, 2646
BstUI	CGCG	1 cut(s)	1729
BstV1I	GCAGC	7 cut(s)	46, 49, 52, 55, 859, 2373, 2691
BstV2I	GAAGAC	2 cut(s)	900, 2078
BstX2I	RGATCY	1 cut(s)	2961
BstXI	CCANNNNNNTGG	1 cut(s)	2204
BstYI	RGATCY	1 cut(s)	2961
BstZ17I	GTATAC	1 cut(s)	1868
BsuI	GTATCC	1 cut(s)	261
BsuRI	GGCC	7 cut(s)	190, 1396, 1927, 2041, 2712, 2988, 3221
BtgI	CCRYGG	3 cut(s)	108, 1221, 3180
BtsI	GCAGTG	1 cut(s)	147
BtsIMutI	CAGTG	6 cut(s)	147, 1360, 1418, 1937, 2793, 3351
BveI	ACCTGC	2 cut(s)	1749, 3207
Cac8I	GCNNGC	2 cut(s)	2952, 3223
CaiI	CAGNNNCTG	2 cut(s)	332, 661
CciI	TCATGA	1 cut(s)	1786
CfoI	GCGC	3 cut(s)	1086, 1702, 1729
Cfr10I	RCCGGY	1 cut(s)	1923
Cfr13I	GGNCC	5 cut(s)	73, 98, 1738, 2039, 2987
CseI	GACGC	3 cut(s)	667, 1303, 1895
CsiI	ACCWGGT	1 cut(s)	3042
CspCI	CAANNNNNGTGG	2 cut(s)	729, 764
DdeI	CTNAG	9 cut(s)	102, 366, 1137, 1637, 2067, 2349, 2528, 2858, 3192
DraI	TTTAAA	2 cut(s)	1420, 1534
DrdI	GACNNNNNNGTC	1 cut(s)	1313
DseDI	GACNNNNNNGTC	1 cut(s)	1313
EaeI	YGGCCR	3 cut(s)	188, 1925, 3219
Eam1104I	CTCTTC	9 cut(s)	654, 1338, 1451, 1485, 1839, 2076, 2118, 2952, 2967
EarI	CTCTTC	9 cut(s)	654, 1338, 1451, 1485, 1839, 2076, 2118, 2952, 2967
EciI	GGCGGA	2 cut(s)	6, 280
Ecl136II	GAGCTC	1 cut(s)	2128
Eco130I	CCWWGG	6 cut(s)	108, 622, 1221, 2748, 3180, 3268
Eco147I	AGGCCT	1 cut(s)	2712
Eco24I	GRGCYC	2 cut(s)	1465, 2130
Eco32I	GATATC	1 cut(s)	2689
Eco47I	GGWCC	3 cut(s)	73, 98, 1738
Eco53kI	GAGCTC	1 cut(s)	2128
Eco57I	CTGAAG	6 cut(s)	638, 755, 1166, 1670, 1716, 2988
EcoICRI	GAGCTC	1 cut(s)	2128
EcoNI	CCTNNNNNAGG	2 cut(s)	504, 1838
EcoO109I	RGGNCCY	1 cut(s)	1738
EcoRII	CCWGG	3 cut(s)	1510, 2397, 3042
EcoRV	GATATC	1 cut(s)	2689
EcoT14I	CCWWGG	6 cut(s)	108, 622, 1221, 2748, 3180, 3268
EcoT22I	ATGCAT	3 cut(s)	533, 832, 974
EcoT38I	GRGCYC	2 cut(s)	1465, 2130
ErhI	CCWWGG	6 cut(s)	108, 622, 1221, 2748, 3180, 3268
FalI	AAGNNNNNCTT	6 cut(s)	1689, 1721, 2019, 2051, 2073, 2105
FaqI	GGGAC	4 cut(s)	59, 1229, 2336, 3122
FauI	CCCGC	3 cut(s)	1206, 1352, 2910
FauNDI	CATATG	2 cut(s)	699, 832
FbaI	TGATCA	3 cut(s)	1408, 2932, 3286
FblI	GTMKAC	1 cut(s)	1867
Fnu4HI	GCNGC	9 cut(s)	20, 23, 35, 38, 41, 44, 848, 2362, 2705
FriOI	GRGCYC	2 cut(s)	1465, 2130
Fsp4HI	GCNGC	9 cut(s)	20, 23, 35, 38, 41, 44, 848, 2362, 2705
FspBI	CTAG	5 cut(s)	26, 177, 501, 786, 1466
GlaI	GCGC	3 cut(s)	1085, 1701, 1728
GluI	GCNGC	9 cut(s)	20, 23, 35, 38, 41, 44, 848, 2362, 2705
HaeII	RGCGCY	1 cut(s)	1087
HaeIII	GGCC	7 cut(s)	190, 1396, 1927, 2041, 2712, 2988, 3221
HapII	CCGG	3 cut(s)	1924, 2042, 2131
HgaI	GACGC	3 cut(s)	667, 1303, 1895
HhaI	GCGC	3 cut(s)	1086, 1702, 1729
Hin1I	GRCGYC	1 cut(s)	855
Hin6I	GCGC	3 cut(s)	1084, 1700, 1727
HinP1I	GCGC	3 cut(s)	1084, 1700, 1727
HincII	GTYRAC	2 cut(s)	1585, 1883
HindII	GTYRAC	2 cut(s)	1585, 1883
HindIII	AAGCTT	3 cut(s)	754, 767, 887
HinfI	GANTC	8 cut(s)	4, 13, 329, 584, 836, 1004, 2086, 3322
HpaII	CCGG	3 cut(s)	1924, 2042, 2131
HphI	GGTGA	2 cut(s)	497, 1106
Hpy99I	CGWCG	2 cut(s)	98, 157
HpyAV	CCTTC	8 cut(s)	938, 1184, 1837, 2149, 2281, 2400, 3019, 3231
HpyCH4III	ACNGT	9 cut(s)	98, 157, 913, 1119, 1355, 1974, 2417, 2797, 3264
HpyCH4IV	ACGT	5 cut(s)	855, 1192, 1576, 1885, 2136
HpyF10VI	GCNNNNNNNGC	8 cut(s)	31, 40, 43, 49, 146, 548, 1372, 3290
HpyF3I	CTNAG	9 cut(s)	102, 366, 1137, 1637, 2067, 2349, 2528, 2858, 3192
HpySE526I	ACGT	5 cut(s)	855, 1192, 1576, 1885, 2136
Hsp92I	GRCGYC	1 cut(s)	855
HspAI	GCGC	3 cut(s)	1084, 1700, 1727
Ksp22I	TGATCA	3 cut(s)	1408, 2932, 3286
LguI	GCTCTTC	1 cut(s)	2118
Lsp1109I	GCAGC	7 cut(s)	46, 49, 52, 55, 859, 2373, 2691
LweI	GCATC	9 cut(s)	57, 136, 518, 744, 817, 981, 1123, 1291, 1812
MabI	ACCWGGT	1 cut(s)	3042
MaeI	CTAG	5 cut(s)	26, 177, 501, 786, 1466
MaeII	ACGT	5 cut(s)	855, 1192, 1576, 1885, 2136
MfeI	CAATTG	2 cut(s)	192, 1022
MflI	RGATCY	1 cut(s)	2961
MhlI	GDGCHC	7 cut(s)	951, 1353, 1377, 1465, 2130, 2646, 2795
MlsI	TGGCCA	2 cut(s)	190, 3221
MluNI	TGGCCA	2 cut(s)	190, 3221
MlyI	GAGTC	2 cut(s)	7, 578
MmeI	TCCRAC	4 cut(s)	108, 176, 589, 1948
Mox20I	TGGCCA	2 cut(s)	190, 3221
Mph1103I	ATGCAT	3 cut(s)	533, 832, 974
MroXI	GAANNNNTTC	1 cut(s)	2085
MscI	TGGCCA	2 cut(s)	190, 3221
MseI	TTAA	8 cut(s)	213, 396, 438, 1419, 1533, 1839, 2820, 3108
MslI	CAYNNNNRTG	7 cut(s)	820, 977, 1380, 2280, 2493, 2639, 2847
Msp20I	TGGCCA	2 cut(s)	190, 3221
MspCI	CTTAAG	1 cut(s)	2819
MspI	CCGG	3 cut(s)	1924, 2042, 2131
MspR9I	CCNGG	3 cut(s)	1512, 2399, 3044
MunI	CAATTG	2 cut(s)	192, 1022
Mva1269I	GAATGC	1 cut(s)	974
MvaI	CCWGG	3 cut(s)	1512, 2399, 3044
MvnI	CGCG	1 cut(s)	1729
MwoI	GCNNNNNNNGC	8 cut(s)	31, 40, 43, 49, 146, 548, 1372, 3290
NcoI	CCATGG	3 cut(s)	108, 1221, 3180
NdeI	CATATG	2 cut(s)	699, 832
NlaIV	GGNNCC	2 cut(s)	75, 950
NmuCI	GTSAC	4 cut(s)	452, 576, 3303, 3326
NsiI	ATGCAT	3 cut(s)	533, 832, 974
NspI	RCATGY	2 cut(s)	1564, 2638
NspV	TTCGAA	1 cut(s)	556
PagI	TCATGA	1 cut(s)	1786
PceI	AGGCCT	1 cut(s)	2712
PciSI	GCTCTTC	1 cut(s)	2118
PcsI	WCGNNNNNNNCGW	1 cut(s)	1804
PctI	GAATGC	1 cut(s)	974
PdmI	GAANNNNTTC	1 cut(s)	2085
PfeI	GAWTC	5 cut(s)	329, 836, 1004, 2086, 3322
PflFI	GACNNNGTC	1 cut(s)	811
PfoI	TCCNGGA	1 cut(s)	2397
PkrI	GCNGC	9 cut(s)	21, 24, 36, 39, 42, 45, 849, 2363, 2706
PleI	GAGTC	2 cut(s)	7, 578
PpsI	GAGTC	2 cut(s)	7, 578
Ppu21I	YACGTR	1 cut(s)	1577
PpuMI	RGGWCCY	1 cut(s)	1738
Psp124BI	GAGCTC	1 cut(s)	2130
Psp1406I	AACGTT	2 cut(s)	1192, 1885
Psp5II	RGGWCCY	1 cut(s)	1738
Psp6I	CCWGG	3 cut(s)	1510, 2397, 3042
PspGI	CCWGG	3 cut(s)	1510, 2397, 3042
PspN4I	GGNNCC	2 cut(s)	75, 950
PspPI	GGNCC	5 cut(s)	73, 98, 1738, 2039, 2987
PspPPI	RGGWCCY	1 cut(s)	1738
PstNI	CAGNNNCTG	2 cut(s)	332, 661
PsuI	RGATCY	1 cut(s)	2961
PsyI	GACNNNGTC	1 cut(s)	811
RseI	CAYNNNNRTG	7 cut(s)	820, 977, 1380, 2280, 2493, 2639, 2847
SacI	GAGCTC	1 cut(s)	2130
SapI	GCTCTTC	1 cut(s)	2118
SaqAI	TTAA	8 cut(s)	213, 396, 438, 1419, 1533, 1839, 2820, 3108
SatI	GCNGC	9 cut(s)	20, 23, 35, 38, 41, 44, 848, 2362, 2705
Sau96I	GGNCC	5 cut(s)	73, 98, 1738, 2039, 2987
ScaI	AGTACT	1 cut(s)	2939
SchI	GAGTC	2 cut(s)	7, 578
ScrFI	CCNGG	3 cut(s)	1512, 2399, 3044
SduI	GDGCHC	7 cut(s)	951, 1353, 1377, 1465, 2130, 2646, 2795
SexAI	ACCWGGT	1 cut(s)	3042
SfaNI	GCATC	9 cut(s)	57, 136, 518, 744, 817, 981, 1123, 1291, 1812
SfuI	TTCGAA	1 cut(s)	556
SinI	GGWCC	3 cut(s)	73, 98, 1738
SmiI	ATTTAAAT	1 cut(s)	1534
SmiMI	CAYNNNNRTG	7 cut(s)	820, 977, 1380, 2280, 2493, 2639, 2847
SmlI	CTYRAG	4 cut(s)	1088, 1949, 2471, 2819
SmoI	CTYRAG	4 cut(s)	1088, 1949, 2471, 2819
SpeI	ACTAGT	1 cut(s)	785
SseBI	AGGCCT	1 cut(s)	2712
SspMI	CTAG	5 cut(s)	26, 177, 501, 786, 1466
SstI	GAGCTC	1 cut(s)	2130
StuI	AGGCCT	1 cut(s)	2712
StyD4I	CCNGG	3 cut(s)	1510, 2397, 3042
StyI	CCWWGG	6 cut(s)	108, 622, 1221, 2748, 3180, 3268
SwaI	ATTTAAAT	1 cut(s)	1534
TaaI	ACNGT	9 cut(s)	98, 157, 913, 1119, 1355, 1974, 2417, 2797, 3264
TaiI	ACGT	5 cut(s)	858, 1195, 1579, 1888, 2139
TaqI	TCGA	5 cut(s)	556, 1062, 1197, 1933, 2611
TatI	WGTACW	4 cut(s)	774, 2665, 2937, 3118
TauI	GCSGC	2 cut(s)	22, 25
TfiI	GAWTC	5 cut(s)	329, 836, 1004, 2086, 3322
Tru1I	TTAA	8 cut(s)	213, 396, 438, 1419, 1533, 1839, 2820, 3108
Tru9I	TTAA	8 cut(s)	213, 396, 438, 1419, 1533, 1839, 2820, 3108
TscAI	CASTG	6 cut(s)	147, 1360, 1418, 1944, 2800, 3351
TseFI	GTSAC	4 cut(s)	452, 576, 3303, 3326
TseI	GCWGC	7 cut(s)	34, 37, 40, 43, 847, 2361, 2704
Tsp45I	GTSAC	4 cut(s)	452, 576, 3303, 3326
TspGWI	ACGGA	4 cut(s)	725, 2288, 2363, 2675
TspRI	CASTG	6 cut(s)	147, 1360, 1418, 1944, 2800, 3351
Tth111I	GACNNNGTC	1 cut(s)	811
Vha464I	CTTAAG	1 cut(s)	2819
VneI	GTGCAC	2 cut(s)	1349, 2642
VpaK11BI	GGWCC	3 cut(s)	73, 98, 1738
XagI	CCTNNNNNAGG	2 cut(s)	504, 1838
XapI	RAATTY	6 cut(s)	337, 1421, 1535, 2231, 3052, 3164
XbaI	TCTAGA	1 cut(s)	1465
XceI	RCATGY	2 cut(s)	1564, 2638
XmiI	GTMKAC	1 cut(s)	1867
XmnI	GAANNNNTTC	1 cut(s)	2085
XspI	CTAG	5 cut(s)	26, 177, 501, 786, 1466
ZraI	GACGTC	1 cut(s)	856
ZrmI	AGTACT	1 cut(s)	2939
Zsp2I	ATGCAT	3 cut(s)	533, 832, 974

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.