Ubiquitin carboxyl-terminal hydrolase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 567 |
| AarI | CACCTGC | 1 cut(s) | 183 |
| Acc36I | ACCTGC | 2 cut(s) | 183, 1210 |
| AccB1I | GGYRCC | 1 cut(s) | 854 |
| AccII | CGCG | 1 cut(s) | 163 |
| AciI | CCGC | 4 cut(s) | 161, 178, 899, 1404 |
| AclWI | GGATC | 2 cut(s) | 240, 728 |
| AcoI | YGGCCR | 4 cut(s) | 1336, 1423, 1458, 1492 |
| AcsI | RAATTY | 3 cut(s) | 167, 372, 551 |
| AcuI | CTGAAG | 3 cut(s) | 1058, 1209, 1301 |
| AfaI | GTAC | 5 cut(s) | 159, 323, 603, 1200, 1283 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 116, 117, 634, 1345, 1363 |
| AgsI | TTSAA | 4 cut(s) | 54, 425, 509, 931 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 207, 239 |
| AleI | CACNNNNGTG | 1 cut(s) | 79 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1140, 1172 |
| AluBI | AGCT | 7 cut(s) | 199, 292, 352, 765, 1187, 1399, 1420 |
| AluI | AGCT | 7 cut(s) | 199, 292, 352, 765, 1187, 1399, 1420 |
| Alw21I | GWGCWC | 1 cut(s) | 1371 |
| Alw26I | GTCTC | 3 cut(s) | 792, 1050, 1320 |
| AlwI | GGATC | 2 cut(s) | 240, 728 |
| AoxI | GGCC | 5 cut(s) | 1336, 1423, 1458, 1492, 1525 |
| ApeKI | GCWGC | 2 cut(s) | 765, 901 |
| ApoI | RAATTY | 3 cut(s) | 167, 372, 551 |
| AspS9I | GGNCC | 4 cut(s) | 483, 1036, 1222, 1526 |
| AsuHPI | GGTGA | 5 cut(s) | 145, 164, 476, 712, 1187 |
| AvaII | GGWCC | 3 cut(s) | 483, 1036, 1222 |
| AxyI | CCTNAGG | 1 cut(s) | 411 |
| BalI | TGGCCA | 2 cut(s) | 1425, 1494 |
| BanI | GGYRCC | 1 cut(s) | 854 |
| BanII | GRGCYC | 1 cut(s) | 1396 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1182, 1214 |
| BbsI | GAAGAC | 2 cut(s) | 1307, 1440 |
| Bbv12I | GWGCWC | 1 cut(s) | 1371 |
| BbvI | GCAGC | 2 cut(s) | 752, 888 |
| BccI | CCATC | 2 cut(s) | 1444, 1503 |
| BceAI | ACGGC | 3 cut(s) | 1296, 1323, 1445 |
| BclI | TGATCA | 3 cut(s) | 466, 1014, 1161 |
| BcoDI | GTCTC | 3 cut(s) | 792, 1050, 1320 |
| BfaI | CTAG | 3 cut(s) | 680, 848, 890 |
| BfuAI | ACCTGC | 2 cut(s) | 183, 1210 |
| BisI | GCNGC | 2 cut(s) | 766, 902 |
| BlpI | GCTNAGC | 2 cut(s) | 1395, 1400 |
| BlsI | GCNGC | 2 cut(s) | 767, 903 |
| BmcAI | AGTACT | 1 cut(s) | 1283 |
| Bme18I | GGWCC | 3 cut(s) | 483, 1036, 1222 |
| BmgT120I | GGNCC | 4 cut(s) | 483, 1036, 1222, 1526 |
| BmiI | GGNNCC | 3 cut(s) | 856, 1149, 1224 |
| BmsI | GCATC | 2 cut(s) | 45, 1492 |
| BpiI | GAAGAC | 2 cut(s) | 1307, 1440 |
| BpmI | CTGGAG | 1 cut(s) | 1385 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1395, 1400 |
| BsaJI | CCNNGG | 2 cut(s) | 12, 1357 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1235, 1265, 1446, 1476 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 116, 117, 634, 1345, 1363 |
| Bse1I | ACTGG | 4 cut(s) | 135, 153, 1039, 1528 |
| Bse21I | CCTNAGG | 1 cut(s) | 411 |
| BseDI | CCNNGG | 2 cut(s) | 12, 1357 |
| BseGI | GGATG | 7 cut(s) | 36, 658, 834, 894, 1048, 1164, 1455 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 116, 117, 634, 1345, 1363 |
| BseMII | CTCAG | 3 cut(s) | 402, 1365, 1409 |
| BseNI | ACTGG | 4 cut(s) | 135, 153, 1039, 1528 |
| BseXI | GCAGC | 2 cut(s) | 752, 888 |
| BsgI | GTGCAG | 2 cut(s) | 1428, 1484 |
| Bsh1236I | CGCG | 1 cut(s) | 163 |
| BshFI | GGCC | 5 cut(s) | 1338, 1425, 1460, 1494, 1527 |
| BshNI | GGYRCC | 1 cut(s) | 854 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1371 |
| BslFI | GGGAC | 5 cut(s) | 141, 200, 530, 566, 1208 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 116, 117, 634, 1345, 1363 |
| BsmAI | GTCTC | 3 cut(s) | 792, 1050, 1320 |
| BsmBI | CGTCTC | 1 cut(s) | 1320 |
| BsmFI | GGGAC | 5 cut(s) | 141, 200, 530, 566, 1208 |
| BsnI | GGCC | 5 cut(s) | 1338, 1425, 1460, 1494, 1527 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1371, 1396 |
| Bsp143I | GATC | 8 cut(s) | 232, 466, 615, 733, 795, 1014, 1062, 1161 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1395, 1400 |
| BspACI | CCGC | 4 cut(s) | 161, 178, 899, 1404 |
| BspANI | GGCC | 5 cut(s) | 1338, 1425, 1460, 1494, 1527 |
| BspCNI | CTCAG | 3 cut(s) | 403, 1366, 1408 |
| BspFNI | CGCG | 1 cut(s) | 163 |
| BspHI | TCATGA | 1 cut(s) | 1498 |
| BspLI | GGNNCC | 3 cut(s) | 856, 1149, 1224 |
| BspMI | ACCTGC | 2 cut(s) | 183, 1210 |
| BspPI | GGATC | 2 cut(s) | 240, 728 |
| BspT107I | GGYRCC | 1 cut(s) | 854 |
| BsrI | ACTGG | 4 cut(s) | 135, 153, 1039, 1528 |
| BssECI | CCNNGG | 2 cut(s) | 12, 1357 |
| BssMI | GATC | 8 cut(s) | 232, 466, 615, 733, 795, 1014, 1062, 1161 |
| BssT1I | CCWWGG | 2 cut(s) | 12, 1357 |
| Bst4CI | ACNGT | 4 cut(s) | 139, 321, 326, 1003 |
| Bst6I | CTCTTC | 3 cut(s) | 411, 1318, 1472 |
| BstC8I | GCNNGC | 2 cut(s) | 715, 915 |
| BstDEI | CTNAG | 7 cut(s) | 200, 411, 783, 1374, 1386, 1395, 1400 |
| BstF5I | GGATG | 7 cut(s) | 36, 658, 834, 894, 1048, 1164, 1455 |
| BstFNI | CGCG | 1 cut(s) | 163 |
| BstKTI | GATC | 8 cut(s) | 235, 469, 618, 736, 798, 1017, 1065, 1164 |
| BstMAI | GTCTC | 3 cut(s) | 792, 1050, 1320 |
| BstMBI | GATC | 8 cut(s) | 232, 466, 615, 733, 795, 1014, 1062, 1161 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 910 |
| BstUI | CGCG | 1 cut(s) | 163 |
| BstV1I | GCAGC | 2 cut(s) | 752, 888 |
| BstV2I | GAAGAC | 2 cut(s) | 1307, 1440 |
| BstX2I | RGATCY | 2 cut(s) | 232, 733 |
| BstYI | RGATCY | 2 cut(s) | 232, 733 |
| Bsu36I | CCTNAGG | 1 cut(s) | 411 |
| BsuRI | GGCC | 5 cut(s) | 1338, 1425, 1460, 1494, 1527 |
| BtsCI | GGATG | 7 cut(s) | 36, 658, 834, 894, 1048, 1164, 1455 |
| BtsI | GCAGTG | 2 cut(s) | 753, 1358 |
| BtsIMutI | CAGTG | 3 cut(s) | 753, 999, 1358 |
| BveI | ACCTGC | 2 cut(s) | 183, 1210 |
| Cac8I | GCNNGC | 2 cut(s) | 715, 915 |
| CciI | TCATGA | 1 cut(s) | 1498 |
| Cfr13I | GGNCC | 4 cut(s) | 483, 1036, 1222, 1526 |
| CseI | GACGC | 1 cut(s) | 1523 |
| Csp6I | GTAC | 5 cut(s) | 158, 322, 602, 1199, 1282 |
| CviAII | CATG | 5 cut(s) | 548, 947, 1289, 1344, 1499 |
| CviQI | GTAC | 5 cut(s) | 158, 322, 602, 1199, 1282 |
| DdeI | CTNAG | 7 cut(s) | 200, 411, 783, 1374, 1386, 1395, 1400 |
| DpnI | GATC | 8 cut(s) | 234, 468, 617, 735, 797, 1016, 1064, 1163 |
| DpnII | GATC | 8 cut(s) | 232, 466, 615, 733, 795, 1014, 1062, 1161 |
| EaeI | YGGCCR | 4 cut(s) | 1336, 1423, 1458, 1492 |
| Eam1104I | CTCTTC | 3 cut(s) | 411, 1318, 1472 |
| EarI | CTCTTC | 3 cut(s) | 411, 1318, 1472 |
| Eco130I | CCWWGG | 2 cut(s) | 12, 1357 |
| Eco24I | GRGCYC | 1 cut(s) | 1396 |
| Eco32I | GATATC | 2 cut(s) | 274, 1487 |
| Eco47I | GGWCC | 3 cut(s) | 483, 1036, 1222 |
| Eco57I | CTGAAG | 3 cut(s) | 1058, 1209, 1301 |
| Eco81I | CCTNAGG | 1 cut(s) | 411 |
| EcoO109I | RGGNCCY | 2 cut(s) | 483, 1222 |
| EcoRV | GATATC | 2 cut(s) | 274, 1487 |
| EcoT14I | CCWWGG | 2 cut(s) | 12, 1357 |
| EcoT38I | GRGCYC | 1 cut(s) | 1396 |
| ErhI | CCWWGG | 2 cut(s) | 12, 1357 |
| Esp3I | CGTCTC | 1 cut(s) | 1320 |
| FaeI | CATG | 5 cut(s) | 551, 950, 1292, 1347, 1502 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 25, 57, 261, 293 |
| FaqI | GGGAC | 5 cut(s) | 141, 200, 530, 566, 1208 |
| FatI | CATG | 5 cut(s) | 547, 946, 1288, 1343, 1498 |
| FauI | CCCGC | 1 cut(s) | 906 |
| FbaI | TGATCA | 3 cut(s) | 466, 1014, 1161 |
| Fnu4HI | GCNGC | 2 cut(s) | 766, 902 |
| FokI | GGATG | 7 cut(s) | 23, 665, 821, 881, 1035, 1151, 1462 |
| FriOI | GRGCYC | 1 cut(s) | 1396 |
| Fsp4HI | GCNGC | 2 cut(s) | 766, 902 |
| FspBI | CTAG | 3 cut(s) | 680, 848, 890 |
| GluI | GCNGC | 2 cut(s) | 766, 902 |
| GsuI | CTGGAG | 1 cut(s) | 1385 |
| HaeIII | GGCC | 5 cut(s) | 1338, 1425, 1460, 1494, 1527 |
| HgaI | GACGC | 1 cut(s) | 1523 |
| Hin1II | CATG | 5 cut(s) | 551, 950, 1292, 1347, 1502 |
| HindIII | AAGCTT | 1 cut(s) | 290 |
| HinfI | GANTC | 3 cut(s) | 750, 970, 1292 |
| HphI | GGTGA | 5 cut(s) | 145, 164, 476, 712, 1187 |
| Hpy166II | GTNNAC | 3 cut(s) | 1081, 1135, 1201 |
| Hpy188I | TCNGA | 9 cut(s) | 196, 534, 749, 1067, 1297, 1320, 1375, 1449, 1476 |
| Hpy188III | TCNNGA | 5 cut(s) | 410, 509, 560, 1159, 1499 |
| Hpy8I | GTNNAC | 3 cut(s) | 1081, 1135, 1201 |
| Hpy99I | CGWCG | 1 cut(s) | 1517 |
| HpyAV | CCTTC | 5 cut(s) | 287, 594, 700, 1027, 1166 |
| HpyCH4III | ACNGT | 4 cut(s) | 139, 321, 326, 1003 |
| HpyCH4IV | ACGT | 2 cut(s) | 238, 1329 |
| HpyCH4V | TGCA | 8 cut(s) | 913, 1058, 1219, 1288, 1343, 1409, 1465, 1505 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 910 |
| HpyF3I | CTNAG | 7 cut(s) | 200, 411, 783, 1374, 1386, 1395, 1400 |
| HpySE526I | ACGT | 2 cut(s) | 238, 1329 |
| Hsp92II | CATG | 5 cut(s) | 551, 950, 1292, 1347, 1502 |
| Ksp22I | TGATCA | 3 cut(s) | 466, 1014, 1161 |
| Kzo9I | GATC | 8 cut(s) | 232, 466, 615, 733, 795, 1014, 1062, 1161 |
| LmnI | GCTCC | 2 cut(s) | 1348, 1366 |
| Lsp1109I | GCAGC | 2 cut(s) | 752, 888 |
| LweI | GCATC | 2 cut(s) | 45, 1492 |
| MaeI | CTAG | 3 cut(s) | 680, 848, 890 |
| MaeII | ACGT | 2 cut(s) | 238, 1329 |
| MaeIII | GTNAC | 1 cut(s) | 133 |
| MalI | GATC | 8 cut(s) | 234, 468, 617, 735, 797, 1016, 1064, 1163 |
| MboI | GATC | 8 cut(s) | 232, 466, 615, 733, 795, 1014, 1062, 1161 |
| MflI | RGATCY | 2 cut(s) | 232, 733 |
| MhlI | GDGCHC | 2 cut(s) | 1371, 1396 |
| MlsI | TGGCCA | 2 cut(s) | 1425, 1494 |
| MluCI | AATT | 8 cut(s) | 167, 372, 551, 720, 799, 818, 1104, 1214 |
| MluNI | TGGCCA | 2 cut(s) | 1425, 1494 |
| MlyI | GAGTC | 1 cut(s) | 744 |
| MmeI | TCCRAC | 2 cut(s) | 121, 945 |
| MnlI | CCTC | 8 cut(s) | 121, 258, 406, 427, 474, 496, 643, 1383 |
| Mox20I | TGGCCA | 2 cut(s) | 1425, 1494 |
| MscI | TGGCCA | 2 cut(s) | 1425, 1494 |
| MseI | TTAA | 8 cut(s) | 288, 497, 555, 576, 821, 1026, 1194, 1534 |
| MslI | CAYNNNNRTG | 3 cut(s) | 79, 1053, 1500 |
| Msp20I | TGGCCA | 2 cut(s) | 1425, 1494 |
| MspA1I | CMGCKG | 1 cut(s) | 901 |
| MvnI | CGCG | 1 cut(s) | 163 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 910 |
| NdeII | GATC | 8 cut(s) | 232, 466, 615, 733, 795, 1014, 1062, 1161 |
| NlaIII | CATG | 5 cut(s) | 551, 950, 1292, 1347, 1502 |
| NlaIV | GGNNCC | 3 cut(s) | 856, 1149, 1224 |
| NmuCI | GTSAC | 1 cut(s) | 133 |
| OliI | CACNNNNGTG | 1 cut(s) | 79 |
| PagI | TCATGA | 1 cut(s) | 1498 |
| PaqCI | CACCTGC | 1 cut(s) | 183 |
| PfeI | GAWTC | 2 cut(s) | 970, 1292 |
| PflFI | GACNNNGTC | 1 cut(s) | 7 |
| PkrI | GCNGC | 2 cut(s) | 767, 903 |
| PleI | GAGTC | 1 cut(s) | 744 |
| PpsI | GAGTC | 1 cut(s) | 744 |
| PpuMI | RGGWCCY | 2 cut(s) | 483, 1222 |
| PsiI | TTATAA | 1 cut(s) | 567 |
| Psp5II | RGGWCCY | 2 cut(s) | 483, 1222 |
| PspN4I | GGNNCC | 3 cut(s) | 856, 1149, 1224 |
| PspPI | GGNCC | 4 cut(s) | 483, 1036, 1222, 1526 |
| PspPPI | RGGWCCY | 2 cut(s) | 483, 1222 |
| PsuI | RGATCY | 2 cut(s) | 232, 733 |
| PsyI | GACNNNGTC | 1 cut(s) | 7 |
| RsaI | GTAC | 5 cut(s) | 159, 323, 603, 1200, 1283 |
| RsaNI | GTAC | 5 cut(s) | 158, 322, 602, 1199, 1282 |
| RseI | CAYNNNNRTG | 3 cut(s) | 79, 1053, 1500 |
| SaqAI | TTAA | 8 cut(s) | 288, 497, 555, 576, 821, 1026, 1194, 1534 |
| SatI | GCNGC | 2 cut(s) | 766, 902 |
| Sau3AI | GATC | 8 cut(s) | 232, 466, 615, 733, 795, 1014, 1062, 1161 |
| Sau96I | GGNCC | 4 cut(s) | 483, 1036, 1222, 1526 |
| ScaI | AGTACT | 1 cut(s) | 1283 |
| SchI | GAGTC | 1 cut(s) | 744 |
| SduI | GDGCHC | 2 cut(s) | 1371, 1396 |
| SfaNI | GCATC | 2 cut(s) | 45, 1492 |
| SinI | GGWCC | 3 cut(s) | 483, 1036, 1222 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 79, 1053, 1500 |
| Sse9I | AATT | 8 cut(s) | 167, 372, 551, 720, 799, 818, 1104, 1214 |
| SsiI | CCGC | 4 cut(s) | 161, 178, 899, 1404 |
| SspI | AATATT | 2 cut(s) | 572, 979 |
| SspMI | CTAG | 3 cut(s) | 680, 848, 890 |
| StyI | CCWWGG | 2 cut(s) | 12, 1357 |
| TaaI | ACNGT | 4 cut(s) | 139, 321, 326, 1003 |
| TaiI | ACGT | 2 cut(s) | 241, 1332 |
| TaqI | TCGA | 2 cut(s) | 493, 1512 |
| TasI | AATT | 8 cut(s) | 167, 372, 551, 720, 799, 818, 1104, 1214 |
| TatI | WGTACW | 2 cut(s) | 1198, 1281 |
| TfiI | GAWTC | 2 cut(s) | 970, 1292 |
| Tru1I | TTAA | 8 cut(s) | 288, 497, 555, 576, 821, 1026, 1194, 1534 |
| Tru9I | TTAA | 8 cut(s) | 288, 497, 555, 576, 821, 1026, 1194, 1534 |
| TscAI | CASTG | 3 cut(s) | 760, 1006, 1358 |
| TseFI | GTSAC | 1 cut(s) | 133 |
| TseI | GCWGC | 2 cut(s) | 765, 901 |
| Tsp45I | GTSAC | 1 cut(s) | 133 |
| TspDTI | ATGAA | 7 cut(s) | 12, 77, 312, 564, 611, 897, 932 |
| TspGWI | ACGGA | 2 cut(s) | 617, 1157 |
| TspRI | CASTG | 3 cut(s) | 760, 1006, 1358 |
| Tth111I | GACNNNGTC | 1 cut(s) | 7 |
| VpaK11BI | GGWCC | 3 cut(s) | 483, 1036, 1222 |
| XapI | RAATTY | 3 cut(s) | 167, 372, 551 |
| XspI | CTAG | 3 cut(s) | 680, 848, 890 |
| ZrmI | AGTACT | 1 cut(s) | 1283 |