Ubiquitin carboxyl-terminal hydrolase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1709 |
| Acc36I | ACCTGC | 1 cut(s) | 581 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 94, 145 |
| AccI | GTMKAC | 3 cut(s) | 349, 1700, 3000 |
| AccII | CGCG | 2 cut(s) | 258, 436 |
| AccIII | TCCGGA | 1 cut(s) | 2833 |
| AciI | CCGC | 6 cut(s) | 59, 243, 317, 1204, 1490, 2552 |
| AclWI | GGATC | 7 cut(s) | 159, 297, 482, 1782, 2096, 2418, 2526 |
| AcsI | RAATTY | 5 cut(s) | 384, 1412, 1526, 3039, 3345 |
| AcuI | CTGAAG | 4 cut(s) | 1311, 2631, 2964, 3179 |
| AcyI | GRCGYC | 1 cut(s) | 14 |
| AfaI | GTAC | 6 cut(s) | 405, 767, 1562, 2152, 2660, 3114 |
| AflII | CTTAAG | 1 cut(s) | 605 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 3211 |
| AjnI | CCWGG | 2 cut(s) | 1501, 1718 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 2010, 2042, 3024, 3056 |
| AleI | CACNNNNGTG | 1 cut(s) | 1214 |
| Alw21I | GWGCWC | 7 cut(s) | 888, 1359, 1668, 2400, 2783, 3276, 3289 |
| Alw26I | GTCTC | 2 cut(s) | 2307, 2832 |
| Alw44I | GTGCAC | 2 cut(s) | 1355, 2396 |
| AlwI | GGATC | 7 cut(s) | 159, 297, 482, 1782, 2096, 2418, 2526 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 652 |
| Ama87I | CYCGRG | 3 cut(s) | 74, 2207, 3108 |
| Aor13HI | TCCGGA | 1 cut(s) | 2833 |
| AoxI | GGCC | 1 cut(s) | 1385 |
| ApaLI | GTGCAC | 2 cut(s) | 1355, 2396 |
| ApeKI | GCWGC | 3 cut(s) | 838, 2162, 3305 |
| ApoI | RAATTY | 5 cut(s) | 384, 1412, 1526, 3039, 3345 |
| AseI | ATTAAT | 2 cut(s) | 1224, 2799 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 929, 2949, 3192 |
| AspA2I | CCTAGG | 1 cut(s) | 3175 |
| AspLEI | GCGC | 2 cut(s) | 258, 436 |
| AspS9I | GGNCC | 2 cut(s) | 2629, 2975 |
| AsuHPI | GGTGA | 6 cut(s) | 77, 1097, 1228, 1487, 2363, 3095 |
| AsuII | TTCGAA | 1 cut(s) | 933 |
| AvaI | CYCGRG | 3 cut(s) | 74, 2207, 3108 |
| AvaII | GGWCC | 2 cut(s) | 2629, 2975 |
| AvrII | CCTAGG | 1 cut(s) | 3175 |
| AxyI | CCTNAGG | 2 cut(s) | 273, 2955 |
| BaeGI | GKGCMC | 3 cut(s) | 1359, 1456, 2400 |
| BanII | GRGCYC | 1 cut(s) | 2250 |
| BbsI | GAAGAC | 2 cut(s) | 1446, 2140 |
| Bbv12I | GWGCWC | 7 cut(s) | 888, 1359, 1668, 2400, 2783, 3276, 3289 |
| BbvI | GCAGC | 3 cut(s) | 850, 2174, 3317 |
| BccI | CCATC | 7 cut(s) | 169, 724, 807, 2276, 2303, 2386, 2733 |
| BciT130I | CCWGG | 2 cut(s) | 1503, 1720 |
| BclI | TGATCA | 3 cut(s) | 28, 1228, 2920 |
| BcoDI | GTCTC | 2 cut(s) | 2307, 2832 |
| BfaI | CTAG | 6 cut(s) | 198, 1457, 2459, 3176, 3216, 3355 |
| BfmI | CTRYAG | 1 cut(s) | 740 |
| BfrI | CTTAAG | 1 cut(s) | 605 |
| BfuAI | ACCTGC | 1 cut(s) | 581 |
| BglII | AGATCT | 1 cut(s) | 3296 |
| BisI | GCNGC | 4 cut(s) | 244, 839, 2163, 3306 |
| BlnI | CCTAGG | 1 cut(s) | 3175 |
| BlsI | GCNGC | 4 cut(s) | 245, 840, 2164, 3307 |
| BmcAI | AGTACT | 1 cut(s) | 2152 |
| Bme1390I | CCNGG | 2 cut(s) | 1503, 1720 |
| Bme18I | GGWCC | 2 cut(s) | 2629, 2975 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 3211 |
| BmeT110I | CYCGRG | 3 cut(s) | 74, 2207, 3108 |
| BmgT120I | GGNCC | 2 cut(s) | 2629, 2975 |
| BmiI | GGNNCC | 2 cut(s) | 2952, 3057 |
| BmrFI | CCNGG | 2 cut(s) | 1503, 1720 |
| BmrI | ACTGGG | 1 cut(s) | 486 |
| BmsI | GCATC | 4 cut(s) | 39, 972, 1114, 2827 |
| BmuI | ACTGGG | 1 cut(s) | 486 |
| BpiI | GAAGAC | 2 cut(s) | 1446, 2140 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2438, 2470 |
| BpmI | CTGGAG | 3 cut(s) | 629, 746, 3290 |
| Bpu14I | TTCGAA | 1 cut(s) | 933 |
| BpuEI | CTTGAG | 4 cut(s) | 902, 1100, 2324, 2450 |
| Bsa29I | ATCGAT | 3 cut(s) | 154, 2271, 2530 |
| BsaBI | GATNNNNATC | 1 cut(s) | 160 |
| BsaHI | GRCGYC | 1 cut(s) | 14 |
| BsaJI | CCNNGG | 8 cut(s) | 93, 534, 613, 1501, 1502, 1719, 2208, 3175 |
| BsaWI | WCCGGW | 1 cut(s) | 2833 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2304, 2334 |
| Bse1I | ACTGG | 9 cut(s) | 143, 424, 492, 569, 763, 1862, 2158, 2933, 3338 |
| Bse21I | CCTNAGG | 2 cut(s) | 273, 2955 |
| Bse3DI | GCAATG | 2 cut(s) | 1603, 1614 |
| Bse8I | GATNNNNATC | 1 cut(s) | 160 |
| BseAI | TCCGGA | 1 cut(s) | 2833 |
| BseBI | CCWGG | 2 cut(s) | 1503, 1720 |
| BseCI | ATCGAT | 3 cut(s) | 154, 2271, 2530 |
| BseDI | CCNNGG | 8 cut(s) | 93, 534, 613, 1501, 1502, 1719, 2208, 3175 |
| BseJI | GATNNNNATC | 1 cut(s) | 160 |
| BseMI | GCAATG | 2 cut(s) | 1603, 1614 |
| BseMII | CTCAG | 2 cut(s) | 1619, 2946 |
| BseNI | ACTGG | 9 cut(s) | 143, 424, 492, 569, 763, 1862, 2158, 2933, 3338 |
| BseRI | GAGGAG | 4 cut(s) | 59, 1475, 1850, 3155 |
| BseSI | GKGCMC | 3 cut(s) | 1359, 1456, 2400 |
| BseXI | GCAGC | 3 cut(s) | 850, 2174, 3317 |
| BseYI | CCCAGC | 1 cut(s) | 2583 |
| Bsh1236I | CGCG | 2 cut(s) | 258, 436 |
| BshFI | GGCC | 1 cut(s) | 1387 |
| BshVI | ATCGAT | 3 cut(s) | 154, 2271, 2530 |
| BsiHKAI | GWGCWC | 7 cut(s) | 888, 1359, 1668, 2400, 2783, 3276, 3289 |
| BsiHKCI | CYCGRG | 3 cut(s) | 74, 2207, 3108 |
| BsiSI | CCGG | 1 cut(s) | 2834 |
| BslFI | GGGAC | 4 cut(s) | 41, 830, 1220, 2360 |
| BsmAI | GTCTC | 2 cut(s) | 2307, 2832 |
| BsmFI | GGGAC | 4 cut(s) | 41, 830, 1220, 2360 |
| BsmI | GAATGC | 3 cut(s) | 61, 965, 2720 |
| BsnI | GGCC | 1 cut(s) | 1387 |
| BsoBI | CYCGRG | 3 cut(s) | 74, 2207, 3108 |
| Bsp119I | TTCGAA | 1 cut(s) | 933 |
| Bsp1286I | GDGCHC | 9 cut(s) | 888, 1359, 1456, 1668, 2250, 2400, 2783, 3276, 3289 |
| Bsp13I | TCCGGA | 1 cut(s) | 2833 |
| Bsp1407I | TGTACA | 1 cut(s) | 1560 |
| Bsp19I | CCATGG | 1 cut(s) | 93 |
| BspACI | CCGC | 6 cut(s) | 59, 243, 317, 1204, 1490, 2552 |
| BspANI | GGCC | 1 cut(s) | 1387 |
| BspCNI | CTCAG | 2 cut(s) | 1620, 2947 |
| BspDI | ATCGAT | 3 cut(s) | 154, 2271, 2530 |
| BspEI | TCCGGA | 1 cut(s) | 2833 |
| BspFNI | CGCG | 2 cut(s) | 258, 436 |
| BspHI | TCATGA | 2 cut(s) | 1033, 1777 |
| BspLI | GGNNCC | 2 cut(s) | 2952, 3057 |
| BspMAI | CTGCAG | 1 cut(s) | 744 |
| BspMI | ACCTGC | 1 cut(s) | 581 |
| BspPI | GGATC | 7 cut(s) | 159, 297, 482, 1782, 2096, 2418, 2526 |
| BspT104I | TTCGAA | 1 cut(s) | 933 |
| BspTI | CTTAAG | 1 cut(s) | 605 |
| BsrDI | GCAATG | 2 cut(s) | 1603, 1614 |
| BsrGI | TGTACA | 1 cut(s) | 1560 |
| BsrI | ACTGG | 9 cut(s) | 143, 424, 492, 569, 763, 1862, 2158, 2933, 3338 |
| BssECI | CCNNGG | 8 cut(s) | 93, 534, 613, 1501, 1502, 1719, 2208, 3175 |
| BssNAI | GTATAC | 2 cut(s) | 1701, 3001 |
| BssNI | GRCGYC | 1 cut(s) | 14 |
| BssT1I | CCWWGG | 3 cut(s) | 93, 613, 3175 |
| Bst1107I | GTATAC | 2 cut(s) | 1701, 3001 |
| Bst2UI | CCWGG | 2 cut(s) | 1503, 1720 |
| Bst6I | CTCTTC | 8 cut(s) | 645, 1476, 2141, 2208, 2439, 2940, 2955, 3226 |
| BstACI | GRCGYC | 1 cut(s) | 14 |
| BstAFI | CTTAAG | 1 cut(s) | 605 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 847 |
| BstAUI | TGTACA | 1 cut(s) | 1560 |
| BstBI | TTCGAA | 1 cut(s) | 933 |
| BstC8I | GCNNGC | 5 cut(s) | 18, 260, 2197, 2296, 2300 |
| BstDSI | CCRYGG | 1 cut(s) | 93 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 1381, 1829, 2228 |
| BstFNI | CGCG | 2 cut(s) | 258, 436 |
| BstHHI | GCGC | 2 cut(s) | 258, 436 |
| BstMAI | GTCTC | 2 cut(s) | 2307, 2832 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 58, 847, 1873, 1882, 2201, 2551 |
| BstNI | CCWGG | 2 cut(s) | 1503, 1720 |
| BstNSI | RCATGY | 2 cut(s) | 356, 1555 |
| BstSCI | CCNGG | 2 cut(s) | 1501, 1718 |
| BstSFI | CTRYAG | 1 cut(s) | 740 |
| BstSLI | GKGCMC | 3 cut(s) | 1359, 1456, 2400 |
| BstUI | CGCG | 2 cut(s) | 258, 436 |
| BstV1I | GCAGC | 3 cut(s) | 850, 2174, 3317 |
| BstV2I | GAAGAC | 2 cut(s) | 1446, 2140 |
| BstX2I | RGATCY | 1 cut(s) | 3296 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 342 |
| BstYI | RGATCY | 1 cut(s) | 3296 |
| BstZ17I | GTATAC | 2 cut(s) | 1701, 3001 |
| Bsu15I | ATCGAT | 3 cut(s) | 154, 2271, 2530 |
| Bsu36I | CCTNAGG | 2 cut(s) | 273, 2955 |
| BsuRI | GGCC | 1 cut(s) | 1387 |
| BsuTUI | ATCGAT | 3 cut(s) | 154, 2271, 2530 |
| BtgI | CCRYGG | 1 cut(s) | 93 |
| BtgZI | GCGATG | 1 cut(s) | 702 |
| BtsI | GCAGTG | 1 cut(s) | 2902 |
| BtsIMutI | CAGTG | 8 cut(s) | 150, 775, 1336, 1409, 1869, 2781, 2902, 3289 |
| BveI | ACCTGC | 1 cut(s) | 581 |
| Cac8I | GCNNGC | 5 cut(s) | 18, 260, 2197, 2296, 2300 |
| CaiI | CAGNNNCTG | 1 cut(s) | 652 |
| CciI | TCATGA | 2 cut(s) | 1033, 1777 |
| CfoI | GCGC | 2 cut(s) | 258, 436 |
| Cfr13I | GGNCC | 2 cut(s) | 2629, 2975 |
| ClaI | ATCGAT | 3 cut(s) | 154, 2271, 2530 |
| CseI | GACGC | 2 cut(s) | 22, 1886 |
| Csp6I | GTAC | 6 cut(s) | 404, 766, 1561, 2151, 2659, 3113 |
| CviQI | GTAC | 6 cut(s) | 404, 766, 1561, 2151, 2659, 3113 |
| DriI | GACNNNNNGTC | 1 cut(s) | 3211 |
| Eam1104I | CTCTTC | 8 cut(s) | 645, 1476, 2141, 2208, 2439, 2940, 2955, 3226 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 3211 |
| EarI | CTCTTC | 8 cut(s) | 645, 1476, 2141, 2208, 2439, 2940, 2955, 3226 |
| Eco130I | CCWWGG | 3 cut(s) | 93, 613, 3175 |
| Eco24I | GRGCYC | 1 cut(s) | 2250 |
| Eco32I | GATATC | 1 cut(s) | 2077 |
| Eco47I | GGWCC | 2 cut(s) | 2629, 2975 |
| Eco57I | CTGAAG | 4 cut(s) | 1311, 2631, 2964, 3179 |
| Eco81I | CCTNAGG | 2 cut(s) | 273, 2955 |
| Eco88I | CYCGRG | 3 cut(s) | 74, 2207, 3108 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 1381, 1829, 2228 |
| EcoRII | CCWGG | 2 cut(s) | 1501, 1718 |
| EcoRV | GATATC | 1 cut(s) | 2077 |
| EcoT14I | CCWWGG | 3 cut(s) | 93, 613, 3175 |
| EcoT22I | ATGCAT | 2 cut(s) | 965, 1549 |
| EcoT38I | GRGCYC | 1 cut(s) | 2250 |
| ErhI | CCWWGG | 3 cut(s) | 93, 613, 3175 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1997, 2029, 2010, 2042 |
| FaqI | GGGAC | 4 cut(s) | 41, 830, 1220, 2360 |
| FauI | CCCGC | 2 cut(s) | 66, 1197 |
| FauNDI | CATATG | 5 cut(s) | 337, 571, 690, 823, 945 |
| FbaI | TGATCA | 3 cut(s) | 28, 1228, 2920 |
| FblI | GTMKAC | 3 cut(s) | 349, 1700, 3000 |
| Fnu4HI | GCNGC | 4 cut(s) | 244, 839, 2163, 3306 |
| FriOI | GRGCYC | 1 cut(s) | 2250 |
| Fsp4HI | GCNGC | 4 cut(s) | 244, 839, 2163, 3306 |
| FspBI | CTAG | 6 cut(s) | 198, 1457, 2459, 3176, 3216, 3355 |
| GlaI | GCGC | 2 cut(s) | 257, 435 |
| GluI | GCNGC | 4 cut(s) | 244, 839, 2163, 3306 |
| GsaI | CCCAGC | 1 cut(s) | 2587 |
| GsuI | CTGGAG | 3 cut(s) | 629, 746, 3290 |
| HaeIII | GGCC | 1 cut(s) | 1387 |
| HapII | CCGG | 1 cut(s) | 2834 |
| HgaI | GACGC | 2 cut(s) | 22, 1886 |
| HhaI | GCGC | 2 cut(s) | 258, 436 |
| Hin1I | GRCGYC | 1 cut(s) | 14 |
| Hin6I | GCGC | 2 cut(s) | 256, 434 |
| HinP1I | GCGC | 2 cut(s) | 256, 434 |
| HincII | GTYRAC | 3 cut(s) | 187, 2337, 2608 |
| HindII | GTYRAC | 3 cut(s) | 187, 2337, 2608 |
| HindIII | AAGCTT | 1 cut(s) | 2093 |
| HpaII | CCGG | 1 cut(s) | 2834 |
| HphI | GGTGA | 6 cut(s) | 77, 1097, 1228, 1487, 2363, 3095 |
| HpyAV | CCTTC | 6 cut(s) | 889, 929, 984, 1744, 2342, 3074 |
| HpyCH4IV | ACGT | 2 cut(s) | 623, 1075 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 58, 847, 1873, 1882, 2201, 2551 |
| HpySE526I | ACGT | 2 cut(s) | 623, 1075 |
| Hsp92I | GRCGYC | 1 cut(s) | 14 |
| HspAI | GCGC | 2 cut(s) | 256, 434 |
| Kpn2I | TCCGGA | 1 cut(s) | 2833 |
| Ksp22I | TGATCA | 3 cut(s) | 28, 1228, 2920 |
| Lsp1109I | GCAGC | 3 cut(s) | 850, 2174, 3317 |
| LweI | GCATC | 4 cut(s) | 39, 972, 1114, 2827 |
| MaeI | CTAG | 6 cut(s) | 198, 1457, 2459, 3176, 3216, 3355 |
| MaeII | ACGT | 2 cut(s) | 623, 1075 |
| MaeIII | GTNAC | 7 cut(s) | 10, 770, 1245, 1429, 1485, 1574, 2561 |
| MflI | RGATCY | 1 cut(s) | 3296 |
| MhlI | GDGCHC | 9 cut(s) | 888, 1359, 1456, 1668, 2250, 2400, 2783, 3276, 3289 |
| MlyI | GAGTC | 5 cut(s) | 569, 643, 754, 1807, 3194 |
| MmeI | TCCRAC | 3 cut(s) | 1939, 2110, 2652 |
| Mph1103I | ATGCAT | 2 cut(s) | 965, 1549 |
| MroI | TCCGGA | 1 cut(s) | 2833 |
| MroXI | GAANNNNTTC | 3 cut(s) | 929, 2949, 3192 |
| MspA1I | CMGCKG | 1 cut(s) | 2165 |
| MspCI | CTTAAG | 1 cut(s) | 605 |
| MspI | CCGG | 1 cut(s) | 2834 |
| MspR9I | CCNGG | 2 cut(s) | 1503, 1720 |
| Mva1269I | GAATGC | 3 cut(s) | 61, 965, 2720 |
| MvaI | CCWGG | 2 cut(s) | 1503, 1720 |
| MvnI | CGCG | 2 cut(s) | 258, 436 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 58, 847, 1873, 1882, 2201, 2551 |
| NcoI | CCATGG | 1 cut(s) | 93 |
| NdeI | CATATG | 5 cut(s) | 337, 571, 690, 823, 945 |
| NlaIV | GGNNCC | 2 cut(s) | 2952, 3057 |
| NmeAIII | GCCGAG | 1 cut(s) | 559 |
| NmuCI | GTSAC | 4 cut(s) | 10, 770, 1429, 1574 |
| NsiI | ATGCAT | 2 cut(s) | 965, 1549 |
| NspI | RCATGY | 2 cut(s) | 356, 1555 |
| NspV | TTCGAA | 1 cut(s) | 933 |
| OliI | CACNNNNGTG | 1 cut(s) | 1214 |
| PaeR7I | CTCGAG | 1 cut(s) | 3108 |
| PagI | TCATGA | 2 cut(s) | 1033, 1777 |
| PasI | CCCWGGG | 1 cut(s) | 1502 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1921 |
| PctI | GAATGC | 3 cut(s) | 61, 965, 2720 |
| PdmI | GAANNNNTTC | 3 cut(s) | 929, 2949, 3192 |
| PfeI | GAWTC | 5 cut(s) | 171, 320, 827, 995, 3316 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 94, 145 |
| PkrI | GCNGC | 4 cut(s) | 245, 840, 2164, 3307 |
| PleI | GAGTC | 5 cut(s) | 569, 643, 753, 1807, 3194 |
| PpsI | GAGTC | 5 cut(s) | 569, 643, 753, 1807, 3194 |
| PshBI | ATTAAT | 2 cut(s) | 1224, 2799 |
| PsiI | TTATAA | 1 cut(s) | 1709 |
| Psp6I | CCWGG | 2 cut(s) | 1501, 1718 |
| PspFI | CCCAGC | 1 cut(s) | 2583 |
| PspGI | CCWGG | 2 cut(s) | 1501, 1718 |
| PspN4I | GGNNCC | 2 cut(s) | 2952, 3057 |
| PspPI | GGNCC | 2 cut(s) | 2629, 2975 |
| PspXI | VCTCGAGB | 1 cut(s) | 3108 |
| PstI | CTGCAG | 1 cut(s) | 744 |
| PstNI | CAGNNNCTG | 1 cut(s) | 652 |
| PsuI | RGATCY | 1 cut(s) | 3296 |
| PvuII | CAGCTG | 1 cut(s) | 2165 |
| RsaI | GTAC | 6 cut(s) | 405, 767, 1562, 2152, 2660, 3114 |
| RsaNI | GTAC | 6 cut(s) | 404, 766, 1561, 2151, 2659, 3113 |
| SatI | GCNGC | 4 cut(s) | 244, 839, 2163, 3306 |
| Sau96I | GGNCC | 2 cut(s) | 2629, 2975 |
| ScaI | AGTACT | 1 cut(s) | 2152 |
| SchI | GAGTC | 5 cut(s) | 569, 643, 754, 1807, 3194 |
| ScrFI | CCNGG | 2 cut(s) | 1503, 1720 |
| SduI | GDGCHC | 9 cut(s) | 888, 1359, 1456, 1668, 2250, 2400, 2783, 3276, 3289 |
| SfaNI | GCATC | 4 cut(s) | 39, 972, 1114, 2827 |
| SfcI | CTRYAG | 1 cut(s) | 740 |
| Sfr274I | CTCGAG | 1 cut(s) | 3108 |
| SfuI | TTCGAA | 1 cut(s) | 933 |
| SinI | GGWCC | 2 cut(s) | 2629, 2975 |
| SlaI | CTCGAG | 1 cut(s) | 3108 |
| SmlI | CTYRAG | 6 cut(s) | 605, 881, 1079, 2303, 2429, 3108 |
| SmoI | CTYRAG | 6 cut(s) | 605, 881, 1079, 2303, 2429, 3108 |
| SsiI | CCGC | 6 cut(s) | 59, 243, 317, 1204, 1490, 2552 |
| SspI | AATATT | 2 cut(s) | 1280, 1538 |
| SspMI | CTAG | 6 cut(s) | 198, 1457, 2459, 3176, 3216, 3355 |
| StyD4I | CCNGG | 2 cut(s) | 1501, 1718 |
| StyI | CCWWGG | 3 cut(s) | 93, 613, 3175 |
| TaiI | ACGT | 2 cut(s) | 626, 1078 |
| TaqI | TCGA | 8 cut(s) | 154, 933, 1264, 1416, 1979, 2271, 2530, 3109 |
| TatI | WGTACW | 5 cut(s) | 765, 1560, 2150, 2658, 3112 |
| TauI | GCSGC | 1 cut(s) | 246 |
| TfiI | GAWTC | 5 cut(s) | 171, 320, 827, 995, 3316 |
| TscAI | CASTG | 8 cut(s) | 150, 775, 1336, 1409, 1869, 2788, 2902, 3289 |
| TseFI | GTSAC | 4 cut(s) | 10, 770, 1429, 1574 |
| TseI | GCWGC | 3 cut(s) | 838, 2162, 3305 |
| Tsp45I | GTSAC | 4 cut(s) | 10, 770, 1429, 1574 |
| TspRI | CASTG | 8 cut(s) | 150, 775, 1336, 1409, 1869, 2788, 2902, 3289 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 94, 145 |
| Vha464I | CTTAAG | 1 cut(s) | 605 |
| VneI | GTGCAC | 2 cut(s) | 1355, 2396 |
| VpaK11BI | GGWCC | 2 cut(s) | 2629, 2975 |
| VspI | ATTAAT | 2 cut(s) | 1224, 2799 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 1381, 1829, 2228 |
| XapI | RAATTY | 5 cut(s) | 384, 1412, 1526, 3039, 3345 |
| XceI | RCATGY | 2 cut(s) | 356, 1555 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2306 |
| XhoI | CTCGAG | 1 cut(s) | 3108 |
| XmaJI | CCTAGG | 1 cut(s) | 3175 |
| XmiI | GTMKAC | 3 cut(s) | 349, 1700, 3000 |
| XmnI | GAANNNNTTC | 3 cut(s) | 929, 2949, 3192 |
| XspI | CTAG | 6 cut(s) | 198, 1457, 2459, 3176, 3216, 3355 |
| ZrmI | AGTACT | 1 cut(s) | 2152 |
| Zsp2I | ATGCAT | 2 cut(s) | 965, 1549 |