Rh5BG335500

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000333
Physical Location & Seq
Reverse (-)
46728337 .. 46742973
14637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG335500.1

Sequence Viewer

Length: 3309 bp
ATGCTTGTTCCAGCCTCGGATTTGCCTGAAAACAATCACCAGCCCATGGATGTTGTTGCACAGCCTGAAAATGCTAATAATGTGGAGAACCAACCAGTAGAAGATCCTCCTTCATCGAGATTCACATGGAGAATTGACAACTTTTCTAGGATGAATACAAAGAAGCTTTATTCTGACATATTTGTTGTTGGTGGCTATAAATGGCGTGTGCTTATTTTTCCCAAGGGAAACAATGTGGATCATTTGTCTATGTATTTGGATGTTGCGGATTCGTCAAATTTGCCATATGGGTGGAGTAGATATGCTCAATTTAGCTTGGGAGTTCTCAATCAACTTCATACCAAGTATTCTGTACGAAAAGACACACAACATCAGTTCAATGCACGGGAAAGTGATTGGGGTTTCACATCCTTTATGCCTCTTGGTGAACTGTACGATCCCAGCAGAGGTTATCTCGTGAATGATGCGGTTATAATTGAAGCGGAAGTTCTCGTTCGTAGGGTTGTAGATTATTGGACATATGACTCAAAGAAGGAAACGGGGTATGTTGGGCTTAAGAACCAAGGAGCAACATGCTATATGAACTCTCTCCTGCAGACTCTGTATCATATTCCTTATTTTAGAAAGGCTGTTTACCATATGCCCACGACTGAGAATGATTTGCCATCACAAAGCATACCATTGGCTCTTCAGAGTCTATTTTACAAACTCCAGTACAGTGATAATAGTGTTGCAACAAAAGAGCTGACAAAGTCTTTTGGATGGGATACATATGATTCATTTATGCAGCATGATGTGCAAGAACTAAATAGGGTCTTGTGTGAAAAACTGGAGGACAAAATGAAGGGAACTGTTGTAGAGGGAACCATACAGAAGTTATTTGAAGGTCACCATATGAACTACATCGAATGCATTAATGTGGACTACAAATCTACAAGAAAGGAGTCATTTTATGACCTTCAGCTCGATGTCAAAGGGTGTCGTGATGTATATGCTTCTTTTGATAAGTATGTAGAAGTTGAACGTCTTGAGGGGGATAACAAGTATCATGCTGAACAGAATGGATTACAGGATGCTAAGAAAGGGGTTCTCTTCATTGACTTTCCACCTGTTCTTCAACTTCAACTGAAACGGTTTGAGTACGACTTTATGCGAGACACCATGGTGAAGATTAATGACCGTTATGAGTTCCCTTTGCAACTTGACCTTGATCGAGATAATGGAAAATATTTATCACCTGAAGCTGATAGGAGTGTTCGCAACCTTTACACACTTCACAGTGTATTGGTACACAGTGGTGGGGTGCATGGTGGACATTACTACGCCTTTATCAGACCGACGCTTTCTGATCAGTGGTACAAATTTGATGATGAGAGGGTAACAAAAGAAGATATAAAGAGGGCACTGGAGGAGCAGTATGGTGGTGAGGAAGAGTTACCGCAGACAAACCCTGGATTTAATAACACTCCTTTCAAATTTACAAAATACTCAAACGCGTACATGCTGGTGTACATACGTGAAAGTGACAAGGATAAAATAATCTGCAATGTTGATGAGAAGGACATTGCTGAACATCTTCGGGAGAGGTTGAAGAAAGAACAAGAAGAAAAGGAGCACAAGAAGAAAGAAAAGGCAGAGGCACACCTATATACTATCATTAAGGTTGCCCGGGACGAGAACCTTCTGGAGCAGATTGGGAAGGATATATATTTTGATCTTGTGGATCATGACAAAGTCAAAAGTTTTCGTATTCAGAAGCAGATGCCTTTCAACCTTTTCAAGGAGGAGGTTGCTAAGGAGTTTGGCATACCAGTGCAATTTCAGCGGTTTTGGCTGTGGGCAAAGCGTCAAAACCATACATATCGTCCAAACCGTCCATTGACACCTGTGGAGGAAACACAATCTGTTGGGCAGTTGAGGGAGGTATCAAACAAGGTTCATAATGCAGAACTGAAGTTGTTCTTGGAAATAGAGCTTGGACCGGATTTACACCCTATTGCTCCACCTGACAAGACAAAGGATGATATTCTGCTTTTCTTTAAGCTTTATGATCCTGAAAAAGAAGAACTACGTTATGTTGGACGGCTTTTTGTGAAGAGTACTGGTAAGCCAGCTGAAATCTTGTCAAAATTAAATGAATTGGCTGGCTATGCCCCTGATGAGGAGATCGACCTTTACGAGGAAATTAAGTATGAGCCCACTGTCATGTGTGAACCCATCGATAAGAAATTCACATTTCGAGCCAGCCAGCTTGAGGATGGAGACATTGTTTGCTTCCAAAAACCAGTTCCGGTTGAAAATGGTCAGCAGTTCCGCTATCCAGACGTCCCATCTTTTCTGGATTTTGTGCACAATCGTCTCGTTGTTCATTTTCGATCGTTTGAGAAACCCAAAGAAGATGATTTCAGCTTAGAATTGTCAAAACTTCATACCTATGATGATGTTGTGGAGCGAGTAGCTCAACAACTTGGTTTGGACGATCCATCCAAAATTAGGCTTACCTCACATAATTGTTACTCCCAGCAACCCAAACCTCAGCCAATTAAGTATCGGGGGGTAGACCGTTTGACTGATATGCTTGTTCACTACAATCAGACTTCGGATATATTGTACTATGAAGTATTAGACATCCCTCTGCCTGAATTACAAGGTTTGAAAACCCTGAAAGTTGCATTCCATCATGCTACCAAGGATGAAGTGGTTATTCACACCATAAGACTGCCAAAACAGAGCACTGTTGGGGATGTAATCAATGATCTTAAAACTAAGGTTGAATTGTCTCATCCAGATGCAGAACTTAGGTTGCTCGAGGTTTTCTATCACAAGATATACAAGGTCTTTCCCCAAAGTGAAAAGATCGAAAACATAAATGATCAATACTGGACGTTACGTGCTGAAGAGGTTCCAGAGGAAGAGAAAAATCTTGGTCCTAATGACCGTGTAATTCATGTCTACCACTTCACTAAAGACACCGCTCAGAATCAAATGCAAATTCAAAACTTTGGGGAACCGTTTTTCTTGGTTATACATGAAGGTGAGACCTTGGCTGAAATTAGAGGTCGGATACAAAAGAAATTACAGGTTGCAGATGAGGAATTTGCGAAGTGGAAGTTTGCATTTCTTTCGCTAGGTCGGCCAGAGTATCTTCAGGACACTGACATCGTCTCTAGTCGGTTCCAGAGAAGAGATGTTTATGGTGCTTGGGAGCAGTATCTTGGCCTGGAGCACTCTGACAGCGCTCCTAAAAGAGCTTATGCAGCTAATCAGAATCGCCACACATTTGAGAAGCCGGTGAAAATTTACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko05168 ko05169 ko05203 map04068 map05168 map05169 map05203
Pfam Domains
Protein Families
MATH UCH USP7_C2 USP7_ICP0_bdg

Protein Analysis

1102

Amino Acids

129.21

Weight (kDa)

5.65

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MATH_2 PF22486 40 - 163 5.1e-39 MATH domain
MATH PF00917 45 - 164 1.5e-17 MATH domain
UCH PF00443 183 - 504 1.5e-59 Ubiquitin carboxyl-terminal hydrolase
UCH_1 PF13423 183 - 458 1.9e-15 Ubiquitin carboxyl-terminal hydrolase
USP7_ICP0_bdg PF12436 608 - 860 3.8e-78 ICP0-binding domain of Ubiquitin-specific protease 7
USP7_C2 PF14533 870 - 1080 1.1e-55 Ubiquitin-specific protease C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000333)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11910 AT3G11910 AT3G11910 AT3G11910 AT5G06600 AT5G06600 AT5G06600
fragaria_vesca FvH4_3g26930 FvH4_3g39132 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220
malus_domestica MD02G1261100.v1.1 MD03G1161100.v1.1 MD07G1059900.v1.1 MD11G1177100.v1.1 MD12G1047900.v1.1 MD12G1048000.v1.1 MD12G1048400.v1.1 MD12G1048500.v1.1 MD12G1048700.v1.1 MD12G1048800.v1.1 MD12G1056200.v1.1 MD14G1046300.v1.1 MD14G1046500.v1.1 MD15G1166900.v1.1
prunus_persica Prupe.2G066200_v2.0.a1 Prupe.2G066200_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1
pyrus_communis pycom02g22320 pycom03g11340 pycom07g04580 pycom11g14290 pycom11g15110 pycom12g04110 pycom12g04140 pycom12g04160 pycom12g04210 pycom12g04220 pycom12g04230 pycom12g04290 pycom12g04320 pycom12g04330 pycom12g04350 pycom12g04360 pycom14g03880 pycom14g03900 pycom16g18750
rosa_chinensis RchiOBHm_Chr1g0324111 RchiOBHm_Chr1g0324241 RchiOBHm_Chr3g0477691 RchiOBHm_Chr5g0049371 RchiOBHm_Chr5g0070531
rosa_laevigata RLG00000008612 RLG00000023698 RLG00000034613 RLG00000036139
rosa_multiflora Rmu_co8271779.1_g000001 Rmu_sc0006187.1_g000014
rosa_roxburghii Rroxscaffold_1G00010670 Rroxscaffold_1G00030450 Rroxscaffold_1G00031950 Rroxscaffold_1G00031970 Rroxscaffold_1G00032320 Rroxscaffold_1G00055490 Rroxscaffold_2G00083610 Rroxscaffold_2G00084480 Rroxscaffold_2G00115610 Rroxscaffold_3G00261320 Rroxscaffold_4G00312220 Rroxscaffold_4G00325820 Rroxscaffold_5G00339170 Rroxscaffold_5G00364030 Rroxscaffold_6G00403750
rosa_rugosa Rorug03G0162700 Rorug03G0162800 Rorug03G0162900 Rorug05G0247700 Rorug05G0406600 Rorug05G0406800
rosa_samantha Rh1AG060300 Rh3AG213000 Rh3BG246600 Rh3CG240400 Rh3DG239700 Rh5AG324400 Rh5AG462100 Rh5BG335500 Rh5BG480200 Rh5BG480300 Rh5CG360200 Rh5CG504500 Rh5CG504600 Rh5DG347800 Rh5DG492200 Rh5DG492300
rosa_wichuraiana Rw3G019270 Rw3G026910 Rw5G030650 Rw5G032610 Rw5G043040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 473
AatII GACGTC 1 cut(s) 2328
AccB7I CCANNNNNTGG 1 cut(s) 46
AccBSI CCGCTC 1 cut(s) 2975
AccI GTMKAC 2 cut(s) 2559, 2952
AccII CGCG 1 cut(s) 1496
AciI CCGC 7 cut(s) 266, 467, 482, 1439, 1825, 2314, 2973
AclWI GGATC 6 cut(s) 98, 246, 431, 1731, 2045, 2474
AcoI YGGCCR 1 cut(s) 3134
AcsI RAATTY 7 cut(s) 277, 1361, 1475, 2228, 2991, 3095, 3297
AcuI CTGAAG 6 cut(s) 674, 944, 1260, 1973, 2916, 3131
AcyI GRCGYC 1 cut(s) 2325
AfeI AGCGCT 1 cut(s) 3238
AfiI CCNNNNNNNGG 7 cut(s) 46, 446, 1780, 2161, 2179, 2254, 2493
AflII CTTAAG 1 cut(s) 554
AflIII ACRYGT 1 cut(s) 1494
AjnI CCWGG 2 cut(s) 1450, 3219
AjuI GAANNNNNNNTTGG 6 cut(s) 1946, 1959, 1978, 1991, 2271, 2303
AleI CACNNNNGTG 2 cut(s) 1163, 1296
Alw21I GWGCWC 4 cut(s) 1617, 2352, 2735, 3228
Alw26I GTCTC 6 cut(s) 1149, 2256, 2363, 2784, 3032, 3169
Alw44I GTGCAC 1 cut(s) 2348
AlwI GGATC 6 cut(s) 98, 246, 431, 1731, 2045, 2474
AlwNI CAGNNNCTG 1 cut(s) 601
Ama87I CYCGRG 2 cut(s) 1668, 2807
Aor51HI AGCGCT 1 cut(s) 3238
AoxI GGCC 2 cut(s) 3134, 3217
ApaLI GTGCAC 1 cut(s) 2348
ApeKI GCWGC 2 cut(s) 787, 3257
ApoI RAATTY 7 cut(s) 277, 1361, 1475, 2228, 2991, 3095, 3297
AseI ATTAAT 2 cut(s) 915, 1173
Asp700I GAANNNNTTC 3 cut(s) 1575, 1958, 2901
AspLEI GCGC 1 cut(s) 3239
AspS9I GGNCC 2 cut(s) 1979, 2927
AsuC2I CCSGG 2 cut(s) 1669, 1670
AsuHPI GGTGA 8 cut(s) 29, 437, 881, 1177, 1227, 1436, 3047, 3304
AvaI CYCGRG 2 cut(s) 1668, 2807
AvaII GGWCC 2 cut(s) 1979, 2927
BaeGI GKGCMC 2 cut(s) 1405, 2352
BanII GRGCYC 1 cut(s) 2199
BauI CACGAG 1 cut(s) 455
Bbv12I GWGCWC 4 cut(s) 1617, 2352, 2735, 3228
BbvCI CCTCAGC 1 cut(s) 2535
BbvI GCAGC 2 cut(s) 799, 3269
BccI CCATC 7 cut(s) 673, 756, 2225, 2252, 2338, 2491, 2685
BceAI ACGGC 1 cut(s) 2099
BcgI CGANNNNNNTGC 2 cut(s) 3105, 3139
BciT130I CCWGG 2 cut(s) 1452, 3221
BciVI GTATCC 2 cut(s) 760, 3057
BclI TGATCA 2 cut(s) 1348, 2872
BcnI CCSGG 2 cut(s) 1669, 1670
BcoDI GTCTC 6 cut(s) 1149, 2256, 2363, 2784, 3032, 3169
BfaI CTAG 3 cut(s) 147, 3128, 3168
BfmI CTRYAG 1 cut(s) 593
BfoI RGCGCY 1 cut(s) 3240
BfrI CTTAAG 1 cut(s) 554
BfuI GTATCC 2 cut(s) 760, 3057
BisI GCNGC 2 cut(s) 788, 3258
BlsI GCNGC 2 cut(s) 789, 3259
BmcAI AGTACT 1 cut(s) 2101
Bme1390I CCNGG 4 cut(s) 1452, 1669, 1670, 3221
Bme18I GGWCC 2 cut(s) 1979, 2927
BmeT110I CYCGRG 2 cut(s) 1668, 2807
BmgT120I GGNCC 2 cut(s) 1979, 2927
BmiI GGNNCC 4 cut(s) 865, 2904, 3009, 3176
BmrFI CCNGG 4 cut(s) 1452, 1669, 1670, 3221
BmsI GCATC 4 cut(s) 454, 1063, 1752, 2779
BplI GAGNNNNNCTC 2 cut(s) 438, 470
BpmI CTGGAG 5 cut(s) 695, 851, 1427, 1706, 3242
Bpu10I CCTNAGC 2 cut(s) 1794, 2535
BpuEI CTTGAG 2 cut(s) 1049, 2273
BpuMI CCSGG 2 cut(s) 1669, 1670
Bsa29I ATCGAT 1 cut(s) 2220
BsaAI YACGTR 2 cut(s) 1517, 2891
BsaHI GRCGYC 1 cut(s) 2325
BsaI GGTCTC 1 cut(s) 3032
BsaJI CCNNGG 9 cut(s) 15, 45, 222, 562, 1161, 1450, 1668, 2688, 3042
BsaWI WCCGGW 2 cut(s) 1981, 2290
BsaXI ACNNNNNCTCC 4 cut(s) 1400, 1430, 2253, 2283
Bsc4I CCNNNNNNNGG 7 cut(s) 46, 446, 1780, 2161, 2179, 2254, 2493
Bse118I RCCGGY 1 cut(s) 3289
Bse1I ACTGG 8 cut(s) 95, 712, 834, 1410, 1811, 2107, 2285, 2885
Bse3DI GCAATG 2 cut(s) 1552, 1563
BseBI CCWGG 2 cut(s) 1452, 3221
BseCI ATCGAT 1 cut(s) 2220
BseDI CCNNGG 9 cut(s) 15, 45, 222, 562, 1161, 1450, 1668, 2688, 3042
BseLI CCNNNNNNNGG 7 cut(s) 46, 446, 1780, 2161, 2179, 2254, 2493
BseMI GCAATG 2 cut(s) 1552, 1563
BseMII CTCAG 3 cut(s) 642, 2549, 2990
BseNI ACTGG 8 cut(s) 95, 712, 834, 1410, 1811, 2107, 2285, 2885
BseRI GAGGAG 3 cut(s) 1424, 1799, 2177
BseSI GKGCMC 2 cut(s) 1405, 2352
BseXI GCAGC 2 cut(s) 799, 3269
BseYI CCCAGC 2 cut(s) 440, 2520
Bsh1236I CGCG 1 cut(s) 1496
Bsh1285I CGRYCG 1 cut(s) 2378
BshFI GGCC 2 cut(s) 3136, 3219
BshVI ATCGAT 1 cut(s) 2220
BsiEI CGRYCG 1 cut(s) 2378
BsiHKAI GWGCWC 4 cut(s) 1617, 2352, 2735, 3228
BsiHKCI CYCGRG 2 cut(s) 1668, 2807
BsiSI CCGG 4 cut(s) 1669, 1982, 2291, 3290
BslFI GGGAC 2 cut(s) 1685, 2312
BslI CCNNNNNNNGG 7 cut(s) 46, 446, 1780, 2161, 2179, 2254, 2493
BsmAI GTCTC 6 cut(s) 1149, 2256, 2363, 2784, 3032, 3169
BsmBI CGTCTC 2 cut(s) 2363, 3169
BsmFI GGGAC 2 cut(s) 1685, 2312
BsmI GAATGC 2 cut(s) 914, 2672
BsnI GGCC 2 cut(s) 3136, 3219
Bso31I GGTCTC 1 cut(s) 3032
BsoBI CYCGRG 2 cut(s) 1668, 2807
Bsp1286I GDGCHC 6 cut(s) 1405, 1617, 2199, 2352, 2735, 3228
Bsp1407I TGTACA 1 cut(s) 1509
Bsp19I CCATGG 2 cut(s) 45, 1161
BspACI CCGC 7 cut(s) 266, 467, 482, 1439, 1825, 2314, 2973
BspANI GGCC 2 cut(s) 3136, 3219
BspCNI CTCAG 3 cut(s) 643, 2548, 2989
BspDI ATCGAT 1 cut(s) 2220
BspFNI CGCG 1 cut(s) 1496
BspHI TCATGA 1 cut(s) 1726
BspLI GGNNCC 4 cut(s) 865, 2904, 3009, 3176
BspMAI CTGCAG 1 cut(s) 597
BspPI GGATC 6 cut(s) 98, 246, 431, 1731, 2045, 2474
BspQI GCTCTTC 1 cut(s) 693
BspTI CTTAAG 1 cut(s) 554
BspTNI GGTCTC 1 cut(s) 3032
BsrBI CCGCTC 1 cut(s) 2975
BsrDI GCAATG 2 cut(s) 1552, 1563
BsrFI RCCGGY 1 cut(s) 3289
BsrGI TGTACA 1 cut(s) 1509
BsrI ACTGG 8 cut(s) 95, 712, 834, 1410, 1811, 2107, 2285, 2885
BssAI RCCGGY 1 cut(s) 3289
BssECI CCNNGG 9 cut(s) 15, 45, 222, 562, 1161, 1450, 1668, 2688, 3042
BssNI GRCGYC 1 cut(s) 2325
BssSI CACGAG 1 cut(s) 455
BssT1I CCWWGG 6 cut(s) 45, 222, 562, 1161, 2688, 3042
Bst2BI CACGAG 1 cut(s) 455
Bst2UI CCWGG 2 cut(s) 1452, 3221
Bst6I CTCTTC 7 cut(s) 693, 1097, 1425, 2090, 2892, 2907, 3178
BstACI GRCGYC 1 cut(s) 2325
BstAFI CTTAAG 1 cut(s) 554
BstAPI GCANNNNNTGC 1 cut(s) 796
BstAUI TGTACA 1 cut(s) 1509
BstBAI YACGTR 2 cut(s) 1517, 2891
BstC8I GCNNGC 4 cut(s) 2112, 2146, 2245, 2249
BstDEI CTNAG 8 cut(s) 651, 1077, 1794, 2410, 2535, 2766, 2798, 2976
BstDSI CCRYGG 2 cut(s) 45, 1161
BstEII GGTNACC 1 cut(s) 887
BstENI CCTNNNNNAGG 2 cut(s) 1778, 2177
BstFNI CGCG 1 cut(s) 1496
BstH2I RGCGCY 1 cut(s) 3240
BstHHI GCGC 1 cut(s) 3239
BstMAI GTCTC 6 cut(s) 1149, 2256, 2363, 2784, 3032, 3169
BstMCI CGRYCG 1 cut(s) 2378
BstMWI GCNNNNNNNGC 6 cut(s) 796, 1822, 1831, 2150, 3133, 3257
BstNI CCWGG 2 cut(s) 1452, 3221
BstNSI RCATGY 2 cut(s) 576, 1504
BstPI GGTNACC 1 cut(s) 887
BstSCI CCNGG 4 cut(s) 1450, 1667, 1668, 3219
BstSFI CTRYAG 1 cut(s) 593
BstSLI GKGCMC 2 cut(s) 1405, 2352
BstUI CGCG 1 cut(s) 1496
BstV1I GCAGC 2 cut(s) 799, 3269
BstX2I RGATCY 1 cut(s) 103
BstXI CCANNNNNNTGG 1 cut(s) 291
BstYI RGATCY 1 cut(s) 103
Bsu15I ATCGAT 1 cut(s) 2220
BsuI GTATCC 2 cut(s) 760, 3057
BsuRI GGCC 2 cut(s) 3136, 3219
BsuTUI ATCGAT 1 cut(s) 2220
BtgI CCRYGG 2 cut(s) 45, 1161
BtsIMutI CAGTG 9 cut(s) 724, 1285, 1300, 1358, 1403, 1818, 2199, 2733, 3153
Cac8I GCNNGC 4 cut(s) 2112, 2146, 2245, 2249
CaiI CAGNNNCTG 1 cut(s) 601
CciI TCATGA 1 cut(s) 1726
CfoI GCGC 1 cut(s) 3239
Cfr10I RCCGGY 1 cut(s) 3289
Cfr13I GGNCC 2 cut(s) 1979, 2927
Cfr9I CCCGGG 1 cut(s) 1668
ClaI ATCGAT 1 cut(s) 2220
CseI GACGC 2 cut(s) 1348, 1835
DdeI CTNAG 8 cut(s) 651, 1077, 1794, 2410, 2535, 2766, 2798, 2976
EaeI YGGCCR 1 cut(s) 3134
Eam1104I CTCTTC 7 cut(s) 693, 1097, 1425, 2090, 2892, 2907, 3178
EarI CTCTTC 7 cut(s) 693, 1097, 1425, 2090, 2892, 2907, 3178
Eco130I CCWWGG 6 cut(s) 45, 222, 562, 1161, 2688, 3042
Eco24I GRGCYC 1 cut(s) 2199
Eco31I GGTCTC 1 cut(s) 3032
Eco47I GGWCC 2 cut(s) 1979, 2927
Eco47III AGCGCT 1 cut(s) 3238
Eco57I CTGAAG 6 cut(s) 674, 944, 1260, 1973, 2916, 3131
Eco88I CYCGRG 2 cut(s) 1668, 2807
Eco91I GGTNACC 1 cut(s) 887
EcoNI CCTNNNNNAGG 2 cut(s) 1778, 2177
EcoO65I GGTNACC 1 cut(s) 887
EcoRII CCWGG 2 cut(s) 1450, 3219
EcoT14I CCWWGG 6 cut(s) 45, 222, 562, 1161, 2688, 3042
EcoT22I ATGCAT 1 cut(s) 914
EcoT38I GRGCYC 1 cut(s) 2199
ErhI CCWWGG 6 cut(s) 45, 222, 562, 1161, 2688, 3042
Esp3I CGTCTC 2 cut(s) 2363, 3169
FalI AAGNNNNNCTT 2 cut(s) 1946, 1978
FaqI GGGAC 2 cut(s) 1685, 2312
FauNDI CATATG 5 cut(s) 286, 520, 639, 772, 894
FbaI TGATCA 2 cut(s) 1348, 2872
FblI GTMKAC 2 cut(s) 2559, 2952
Fnu4HI GCNGC 2 cut(s) 788, 3258
FriOI GRGCYC 1 cut(s) 2199
Fsp4HI GCNGC 2 cut(s) 788, 3258
FspBI CTAG 3 cut(s) 147, 3128, 3168
GlaI GCGC 1 cut(s) 3238
GluI GCNGC 2 cut(s) 788, 3258
GsaI CCCAGC 2 cut(s) 444, 2524
GsuI CTGGAG 5 cut(s) 695, 851, 1427, 1706, 3242
HaeII RGCGCY 1 cut(s) 3240
HaeIII GGCC 2 cut(s) 3136, 3219
HapII CCGG 4 cut(s) 1669, 1982, 2291, 3290
HgaI GACGC 2 cut(s) 1348, 1835
HhaI GCGC 1 cut(s) 3239
Hin1I GRCGYC 1 cut(s) 2325
Hin6I GCGC 1 cut(s) 3237
HinP1I GCGC 1 cut(s) 3237
HindIII AAGCTT 2 cut(s) 164, 2042
HinfI GANTC 9 cut(s) 120, 269, 524, 598, 694, 776, 944, 2980, 3268
HpaII CCGG 4 cut(s) 1669, 1982, 2291, 3290
HphI GGTGA 8 cut(s) 29, 437, 881, 1177, 1227, 1436, 3047, 3304
Hpy99I CGWCG 1 cut(s) 1342
HpyAV CCTTC 9 cut(s) 120, 526, 838, 878, 968, 1552, 1691, 1693, 3026
HpyCH4IV ACGT 6 cut(s) 1024, 1516, 2071, 2325, 2885, 2890
HpyF10VI GCNNNNNNNGC 6 cut(s) 796, 1822, 1831, 2150, 3133, 3257
HpyF3I CTNAG 8 cut(s) 651, 1077, 1794, 2410, 2535, 2766, 2798, 2976
HpySE526I ACGT 6 cut(s) 1024, 1516, 2071, 2325, 2885, 2890
Hsp92I GRCGYC 1 cut(s) 2325
HspAI GCGC 1 cut(s) 3237
Ksp22I TGATCA 2 cut(s) 1348, 2872
LguI GCTCTTC 1 cut(s) 693
LmnI GCTCC 9 cut(s) 566, 1411, 1612, 1687, 2005, 2449, 3205, 3223, 3244
Lsp1109I GCAGC 2 cut(s) 799, 3269
LweI GCATC 4 cut(s) 454, 1063, 1752, 2779
MaeI CTAG 3 cut(s) 147, 3128, 3168
MaeII ACGT 6 cut(s) 1024, 1516, 2071, 2325, 2885, 2890
MaeIII GTNAC 6 cut(s) 887, 1378, 1434, 1523, 2513, 2886
MbiI CCGCTC 1 cut(s) 2975
MflI RGATCY 1 cut(s) 103
MhlI GDGCHC 6 cut(s) 1405, 1617, 2199, 2352, 2735, 3228
MluI ACGCGT 1 cut(s) 1494
MlyI GAGTC 4 cut(s) 518, 592, 703, 953
MmeI TCCRAC 2 cut(s) 2059, 3041
Mph1103I ATGCAT 1 cut(s) 914
MroXI GAANNNNTTC 3 cut(s) 1575, 1958, 2901
MspA1I CMGCKG 2 cut(s) 1825, 2114
MspCI CTTAAG 1 cut(s) 554
MspI CCGG 4 cut(s) 1669, 1982, 2291, 3290
MspR9I CCNGG 4 cut(s) 1452, 1669, 1670, 3221
Mva1269I GAATGC 2 cut(s) 914, 2672
MvaI CCWGG 2 cut(s) 1452, 3221
MvnI CGCG 1 cut(s) 1496
MwoI GCNNNNNNNGC 6 cut(s) 796, 1822, 1831, 2150, 3133, 3257
NciI CCSGG 2 cut(s) 1669, 1670
NcoI CCATGG 2 cut(s) 45, 1161
NdeI CATATG 5 cut(s) 286, 520, 639, 772, 894
NlaIV GGNNCC 4 cut(s) 865, 2904, 3009, 3176
NmuCI GTSAC 2 cut(s) 887, 1523
NsiI ATGCAT 1 cut(s) 914
NspI RCATGY 2 cut(s) 576, 1504
OliI CACNNNNGTG 2 cut(s) 1163, 1296
PaeR7I CTCGAG 1 cut(s) 2807
PagI TCATGA 1 cut(s) 1726
PciSI GCTCTTC 1 cut(s) 693
PcsI WCGNNNNNNNCGW 2 cut(s) 1870, 2175
PctI GAATGC 2 cut(s) 914, 2672
PdmI GAANNNNTTC 3 cut(s) 1575, 1958, 2901
PfeI GAWTC 5 cut(s) 120, 269, 776, 2980, 3268
PflFI GACNNNGTC 3 cut(s) 751, 1733, 3161
PflMI CCANNNNNTGG 1 cut(s) 46
PkrI GCNGC 2 cut(s) 789, 3259
Ple19I CGATCG 1 cut(s) 2378
PleI GAGTC 4 cut(s) 518, 592, 702, 952
PpsI GAGTC 4 cut(s) 518, 592, 702, 952
Ppu21I YACGTR 2 cut(s) 1517, 2891
PshBI ATTAAT 2 cut(s) 915, 1173
PsiI TTATAA 1 cut(s) 473
Psp6I CCWGG 2 cut(s) 1450, 3219
PspEI GGTNACC 1 cut(s) 887
PspFI CCCAGC 2 cut(s) 440, 2520
PspGI CCWGG 2 cut(s) 1450, 3219
PspN4I GGNNCC 4 cut(s) 865, 2904, 3009, 3176
PspPI GGNCC 2 cut(s) 1979, 2927
PspXI VCTCGAGB 1 cut(s) 2807
PstI CTGCAG 1 cut(s) 597
PstNI CAGNNNCTG 1 cut(s) 601
PsuI RGATCY 1 cut(s) 103
PsyI GACNNNGTC 3 cut(s) 751, 1733, 3161
PvuI CGATCG 1 cut(s) 2378
PvuII CAGCTG 1 cut(s) 2114
SapI GCTCTTC 1 cut(s) 693
SatI GCNGC 2 cut(s) 788, 3258
Sau96I GGNCC 2 cut(s) 1979, 2927
ScaI AGTACT 1 cut(s) 2101
SchI GAGTC 4 cut(s) 518, 592, 703, 953
ScrFI CCNGG 4 cut(s) 1452, 1669, 1670, 3221
SduI GDGCHC 6 cut(s) 1405, 1617, 2199, 2352, 2735, 3228
SfaNI GCATC 4 cut(s) 454, 1063, 1752, 2779
SfcI CTRYAG 1 cut(s) 593
Sfr274I CTCGAG 1 cut(s) 2807
SinI GGWCC 2 cut(s) 1979, 2927
SlaI CTCGAG 1 cut(s) 2807
SmaI CCCGGG 1 cut(s) 1670
SmlI CTYRAG 4 cut(s) 554, 1028, 2252, 2807
SmoI CTYRAG 4 cut(s) 554, 1028, 2252, 2807
SsiI CCGC 7 cut(s) 266, 467, 482, 1439, 1825, 2314, 2973
SspI AATATT 1 cut(s) 1229
SspMI CTAG 3 cut(s) 147, 3128, 3168
StyD4I CCNGG 4 cut(s) 1450, 1667, 1668, 3219
StyI CCWWGG 6 cut(s) 45, 222, 562, 1161, 2688, 3042
TaiI ACGT 6 cut(s) 1027, 1519, 2074, 2328, 2888, 2893
TaqII GACCGA 1 cut(s) 1351
TatI WGTACW 4 cut(s) 714, 1509, 2099, 2610
TfiI GAWTC 5 cut(s) 120, 269, 776, 2980, 3268
TscAI CASTG 9 cut(s) 724, 1285, 1300, 1358, 1410, 1818, 2206, 2740, 3160
TseFI GTSAC 2 cut(s) 887, 1523
TseI GCWGC 2 cut(s) 787, 3257
Tsp45I GTSAC 2 cut(s) 887, 1523
TspMI CCCGGG 1 cut(s) 1668
TspRI CASTG 9 cut(s) 724, 1285, 1300, 1358, 1410, 1818, 2206, 2740, 3160
Tth111I GACNNNGTC 3 cut(s) 751, 1733, 3161
Van91I CCANNNNNTGG 1 cut(s) 46
Vha464I CTTAAG 1 cut(s) 554
VneI GTGCAC 1 cut(s) 2348
VpaK11BI GGWCC 2 cut(s) 1979, 2927
VspI ATTAAT 2 cut(s) 915, 1173
XagI CCTNNNNNAGG 2 cut(s) 1778, 2177
XapI RAATTY 7 cut(s) 277, 1361, 1475, 2228, 2991, 3095, 3297
XceI RCATGY 2 cut(s) 576, 1504
XcmI CCANNNNNNNNNTGG 2 cut(s) 2255, 2695
XhoI CTCGAG 1 cut(s) 2807
XmaI CCCGGG 1 cut(s) 1668
XmiI GTMKAC 2 cut(s) 2559, 2952
XmnI GAANNNNTTC 3 cut(s) 1575, 1958, 2901
XspI CTAG 3 cut(s) 147, 3128, 3168
ZraI GACGTC 1 cut(s) 2326
ZrmI AGTACT 1 cut(s) 2101
Zsp2I ATGCAT 1 cut(s) 914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.