Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0000333

Physical Location & Seq

Genomic Coordinates Reverse (-)

23076420 .. 23088025

Gene Structure 11606 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG239700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3345 bp

ATGACTATGATGACTCCTCCACCTGTAGATCAAGAGGACGAGGAGATGCTGGTGCCGCATACGGATTTGGTCGAAGGTCCTCAGCCCATGGAAGTAGCTCAAGTGGAGCCGGCGAGTACAGTTGATAATCAGCCAGTGGAGGAGCCTCCAACCATGAAGTTCACTTGGACAATTGAGAGCTTTTCGAGGTTGAACACCAAAAAGCACTACTCTGACATGTTCATCGTCGGTGGTTATAAATGGCGGATACTGATTTTTCCTAAAGGAAACAATGTGGACTACTTGTCAATGTATTTGGATGTGGCGGATTCGCAGGCGCTGCCTTATGGTTGGAGTAGATATGCGCAGTTTAGCTTGGCTATAGTTAATCAGATTCACAACAAGTACTCAATTAGAAAGGACACACAGCACCAATTCAATGCACGAGAAAGTGATTGGGGATTCACATCATTCATGCCTCTCAGTGATCTTTATGACCCCAGCAGGGGTTACCTGGTAAATGATACAGTCGTTGTTGAAGCCGAGGTTGTTGTCCGTAAGGTCCTTGATTACTGGTCGTATGACTCAAAAAAGGAAACTGGTTATGTTGGGCTCAAGAATCAGGGAGCAACTTGTTATATGAATTCACTCCTCCAGACTTTATACCATATACCTTACTTCAGAAAGGCTGTGTATCATATGCCAACAACTGAGAATGACATGCCTTCAGGAAGCATCCCTTTGGCTCTTCAAAGTTTATTCTATAAGCTTCAATACAACGAGAGCAGTGTTGCAACTAAAGAATTAACCAAGTCTTTTGGATGGGATACGTATGATTCGTTTATGCAACATGATGTGCAGGAACTCAATAGAGTTCTATGTGAAAAGCTTGAGGATAAAATGAAGGGAACTGTGGTGGAGGGTACAATACAGCAGTTATTTGAAGGACATCACATGAATTACATTGAATGCATCAATGTGGACTACAAATCTACAAGAAAAGAGTCATTTTATGATCTCCAGCTTGATGTAAAAGGATGTCGTGATGTTTACGCTTCTTTTGACAAGTATGTTGAAGTTGAACGTCTTGAGGGTGACAATAAGTACCATGCTGAAGAACATGGTTTGCAGGATGCTAAGAAGGGTGTCCTGTTTATTGACTTCCCTCCAGTTCTTCAGCTTCAGCTAAAGCGATTTGAGTATGATTTTATGAGGGACACTATGGTAAAGATAAATGATCGCTATGAATTTCCTCTTCAACTTGACCTTGATAGGGAGAATGGCAAATATCTGTCACCTGAATCAGATAAGAGTGTGCGGAACCTCTACACTCTTCATAGTGTCTTAGTTCATAGTGGTGGCGTGCATGGAGGACATTATTATGCTTTTATCAGGCCAACCCTTACTGATCAGTGGTATAAGTTTGATGATGAACGCGTGACAAAAGAGGATATGAAGAGGGCATTAGAGGAACAGTATGGTGGTGAGGAAGAGATGCCCCAGACCAATCCTGGTTTTAATAATACTCCTTTCAAGTTTACAAAATACTCGAACGCATACATGCTTGTGTACATCCGGGACAGTGACAAGGACAAAATAATATGTGATGTGGATGAGAAGGACATAGCTGAACACCTAAGGATAAGGTTGAAGAAAGAACAAGAGGAGAAGGAGGACAAGAGGAGATATAAAGCACAGGCCCATCTTTATACTATTATTAAGGTTGCTCGAGATGAGGACCTAGCAGAACAAATTGGAAGGGACATATATTTTGACCTTGTGGACCATGACAAAGTTCGTAGTTTTCGAATCCAGAAACAGACACCCTTTAACCTTTTCAAGGAGGAAGTTGCGAAAGAGTTGGGGATACCAGTGCAATTTCAGCGATTCTGGATTTGGGCCAAGAGGCAAAACCACACATATCGTCCCAACCGACCATTGACACCCCAGGAAGAATTACAAACAGTTGGACAATTGAGGGAGGCATCAAATAAAACACACAATGCAGAGCTCAAGTTGTTTTTGGAAGTAGAGCTTGGGCCGGAGAAAATTCCTATTCCTCCACCAGACAAAACCAAGGATGATATTCTGCTTTTCTTTAAGCTTTATGAACCTGAGAAACGAGAATTACGGTTTGTTGGTCGGCTTTTTGTTAAGAGTTCTACCAAGCCAATAAAAATAATAGCAAAAATTAATCAAATGGCTGGTTTTGCTCCTGATGAAGAAATTGAACTCTATGAGGAAATAAAGTTTGAGCCTTGTATCATGTGTGAACATCTTGACAAGAGGACCTCATTTCGATTAAGTCAGATTGAAGATGGGGACATTATATGCTTTCAAAAACCTACTCCACTTGAAAGTGAAGAATGTAAATATCCTGACGTCCCTTCATTTTTGGAATATGTACACAACCGTCAGATTGTTCATTTTCGCTCTTTGGAGAAACCAAAAGAGGATGATTTTTCTTTAGAATTGTCAAAGCTACACACTTATGATGATGTGGTGGAGAAGGTGGCTTCCCAAATTGGTTTGGATGATCCTACCAAAATCAGACTCACTGCGCATAACTGCTATTCTCAACAACCTAAACCCCAACCCATCAAATATCGAGGAGTAGAGCATTTAACAGATATGCTGGTCCACTATAATCAAAGTTCTGATATTTTGTATTATGAAGTCTTGGACATCCCCCTGCCAGAATTGCAAGGCCTAAAAAATCTGAAAGTTGCATTTCATCATGCTACAAAAGATGAGGTGGTGATTCACAATATAAGATTGCCCAAGCAGAGCACTGTTGGGGATGTGATTAATGTGCTAAAAACGAAGGTAGAGCTATCTCAACCAAATGCAGAACTCCGACTTCTTGAGGTTTTCTATCACAAGATCTACAAGATCTTCCCACACAGTGAAAAAATTGAGAATATAAATGACCAGTACTGGACATTGCGTGCAGAGGAGATTCCAGAAGAAGAGAAAAACTTGGGTCTTCATGACCGCTTGATTCATGTTTACCACTTTACAAAAGAGACTGCACAGAACCAGATGCAGGTACAGAATTTTGGGGAACCTTTCTTCTTGGTTATTCATGAAGGTGAAACTTTAGCTGAAGTGAAGGCACGCATACAAAAGAAATTGCAGGTCCCTGATGAAGAGTTCGCAAAGTGGAAGTTTGCATTCTTGTCATTGGGTCGTCCCGAGTACCTGCAGGACTCCGATATAGTTTCCAGTCGTTTTCAGAGGAGAGATGTTTATGGAGCTTGGGAGCAGTACCTGGGGTTGGAGCATGCTGATACTGCTCCTAAGAGATCATATGCAGTAAATCAGAACCGTCACACATATGAGAAGCCAGTTAAAATATACAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004843 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009867 GO:0009987 GO:0010033 GO:0016787 GO:0019538 GO:0019783 GO:0023052 GO:0032870 GO:0036459 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070011 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0071704 GO:0101005 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04068 ko05168 ko05169 ko05203 map04068 map05168 map05169 map05203

Pfam Domains

Protein Families

MATH UCH USP7_C2 USP7_ICP0_bdg

Protein Analysis

1114

Amino Acids

130.89

Weight (kDa)

5.62

Isoelectric Point (pI)

45.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MATH_2	PF22486	53 - 176	1.9e-39	MATH domain
MATH	PF00917	58 - 178	3e-18	MATH domain
UCH	PF00443	196 - 517	9.2e-60	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	196 - 471	4.9e-15	Ubiquitin carboxyl-terminal hydrolase
USP7_ICP0_bdg	PF12436	621 - 872	6.3e-78	ICP0-binding domain of Ubiquitin-specific protease 7
USP7_C2	PF14533	882 - 1092	3.6e-57	Ubiquitin-specific protease C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0000333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11910 AT3G11910 AT3G11910 AT3G11910 AT5G06600 AT5G06600 AT5G06600
fragaria_vesca	FvH4_3g26930 FvH4_3g39132 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220
malus_domestica	MD02G1261100.v1.1 MD03G1161100.v1.1 MD07G1059900.v1.1 MD11G1177100.v1.1 MD12G1047900.v1.1 MD12G1048000.v1.1 MD12G1048400.v1.1 MD12G1048500.v1.1 MD12G1048700.v1.1 MD12G1048800.v1.1 MD12G1056200.v1.1 MD14G1046300.v1.1 MD14G1046500.v1.1 MD15G1166900.v1.1
prunus_persica	Prupe.2G066200_v2.0.a1 Prupe.2G066200_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1
pyrus_communis	pycom02g22320 pycom03g11340 pycom07g04580 pycom11g14290 pycom11g15110 pycom12g04110 pycom12g04140 pycom12g04160 pycom12g04210 pycom12g04220 pycom12g04230 pycom12g04290 pycom12g04320 pycom12g04330 pycom12g04350 pycom12g04360 pycom14g03880 pycom14g03900 pycom16g18750
rosa_chinensis	RchiOBHm_Chr1g0324111 RchiOBHm_Chr1g0324241 RchiOBHm_Chr3g0477691 RchiOBHm_Chr5g0049371 RchiOBHm_Chr5g0070531
rosa_laevigata	RLG00000008612 RLG00000023698 RLG00000034613 RLG00000036139
rosa_multiflora	Rmu_co8271779.1_g000001 Rmu_sc0006187.1_g000014
rosa_roxburghii	Rroxscaffold_1G00010670 Rroxscaffold_1G00030450 Rroxscaffold_1G00031950 Rroxscaffold_1G00031970 Rroxscaffold_1G00032320 Rroxscaffold_1G00055490 Rroxscaffold_2G00083610 Rroxscaffold_2G00084480 Rroxscaffold_2G00115610 Rroxscaffold_3G00261320 Rroxscaffold_4G00312220 Rroxscaffold_4G00325820 Rroxscaffold_5G00339170 Rroxscaffold_5G00364030 Rroxscaffold_6G00403750
rosa_rugosa	Rorug03G0162700 Rorug03G0162800 Rorug03G0162900 Rorug05G0247700 Rorug05G0406600 Rorug05G0406800
rosa_samantha	Rh1AG060300 Rh3AG213000 Rh3BG246600 Rh3CG240400 Rh3DG239700 Rh5AG324400 Rh5AG462100 Rh5BG335500 Rh5BG480200 Rh5BG480300 Rh5CG360200 Rh5CG504500 Rh5CG504600 Rh5DG347800 Rh5DG492200 Rh5DG492300
rosa_wichuraiana	Rw3G019270 Rw3G026910 Rw5G030650 Rw5G032610 Rw5G043040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	237
AatII	GACGTC	1 cut(s)	2364
Acc16I	TGCGCA	2 cut(s)	345, 2541
Acc36I	ACCTGC	3 cut(s)	3016, 3106, 3189
AccB1I	GGYRCC	1 cut(s)	52
AccII	CGCG	1 cut(s)	1416
AciI	CCGC	5 cut(s)	56, 244, 305, 1297, 2974
AclWI	GGATC	1 cut(s)	2510
AcsI	RAATTY	4 cut(s)	622, 1226, 2028, 3034
AcuI	CTGAAG	6 cut(s)	643, 690, 1113, 1139, 1145, 3105
AcyI	GRCGYC	1 cut(s)	2361
AdeI	CACNNNGTG	1 cut(s)	2885
AfiI	CCNNNNNNNGG	6 cut(s)	484, 485, 1252, 1819, 3025, 3256
AflIII	ACRYGT	2 cut(s)	216, 1414
AhdI	GACNNNNNGTC	1 cut(s)	283
AjnI	CCWGG	4 cut(s)	492, 1489, 1926, 3249
AjuI	GAANNNNNNNTTGG	2 cut(s)	1998, 2030
Alw21I	GWGCWC	2 cut(s)	1992, 2771
Alw26I	GTCTC	1 cut(s)	2999
AlwI	GGATC	1 cut(s)	2510
AlwNI	CAGNNNCTG	2 cut(s)	319, 3250
Ama87I	CYCGRG	2 cut(s)	1707, 3173
AoxI	GGCC	5 cut(s)	1373, 1677, 1878, 2018, 2686
ApeKI	GCWGC	1 cut(s)	319
ApoI	RAATTY	4 cut(s)	622, 1226, 2028, 3034
ArsI	GACNNNNNNTTYG	2 cut(s)	1733, 1765
AseI	ATTAAT	2 cut(s)	2172, 2787
AspLEI	GCGC	3 cut(s)	319, 346, 2542
AsuC2I	CCSGG	1 cut(s)	1556
AsuHPI	GGTGA	5 cut(s)	1085, 1266, 1475, 2749, 3083
AsuII	TTCGAA	1 cut(s)	1786
AvaI	CYCGRG	2 cut(s)	1707, 3173
AvaII	GGWCC	7 cut(s)	77, 541, 1717, 1762, 2268, 2617, 3118
AxyI	CCTNAGG	1 cut(s)	1616
BaeI	ACNNNNGTAYC	2 cut(s)	495, 528
BanI	GGYRCC	1 cut(s)	52
BanII	GRGCYC	2 cut(s)	594, 1992
BauI	CACGAG	1 cut(s)	423
BbsI	GAAGAC	1 cut(s)	2957
Bbv12I	GWGCWC	2 cut(s)	1992, 2771
BbvCI	CCTCAGC	1 cut(s)	81
BbvI	GCAGC	1 cut(s)	306
BccI	CCATC	4 cut(s)	795, 1689, 2291, 2585
BciT130I	CCWGG	4 cut(s)	494, 1491, 1928, 3251
BciVI	GTATCC	3 cut(s)	240, 799, 1839
BclI	TGATCA	1 cut(s)	1387
BcnI	CCSGG	1 cut(s)	1556
BcoDI	GTCTC	1 cut(s)	2999
BfaI	CTAG	1 cut(s)	1721
BfmI	CTRYAG	3 cut(s)	24, 360, 3182
BfoI	RGCGCY	1 cut(s)	320
BfuAI	ACCTGC	3 cut(s)	3016, 3106, 3189
BfuI	GTATCC	3 cut(s)	240, 799, 1839
BglII	AGATCT	2 cut(s)	2862, 2871
BisI	GCNGC	2 cut(s)	56, 320
BlsI	GCNGC	2 cut(s)	57, 321
BmcAI	AGTACT	2 cut(s)	386, 2915
Bme1390I	CCNGG	5 cut(s)	494, 1491, 1556, 1928, 3251
Bme18I	GGWCC	7 cut(s)	77, 541, 1717, 1762, 2268, 2617, 3118
BmeRI	GACNNNNNGTC	1 cut(s)	283
BmeT110I	CYCGRG	2 cut(s)	1707, 3173
BmiI	GGNNCC	6 cut(s)	54, 108, 144, 1301, 3045, 3120
BmrFI	CCNGG	5 cut(s)	494, 1491, 1556, 1928, 3251
BmsI	GCATC	7 cut(s)	36, 723, 960, 1102, 1464, 1973, 3012
BpiI	GAAGAC	1 cut(s)	2957
BpmI	CTGGAG	3 cut(s)	617, 983, 1131
Bpu10I	CCTNAGC	1 cut(s)	81
Bpu14I	TTCGAA	1 cut(s)	1786
BpuEI	CTTGAG	6 cut(s)	84, 578, 890, 1088, 1976, 2864
BpuMI	CCSGG	1 cut(s)	1556
BsaAI	YACGTR	1 cut(s)	810
BsaBI	GATNNNNATC	1 cut(s)	445
BsaHI	GRCGYC	1 cut(s)	2361
BsaJI	CCNNGG	5 cut(s)	87, 522, 1926, 2055, 3250
Bsc4I	CCNNNNNNNGG	6 cut(s)	484, 485, 1252, 1819, 3025, 3256
Bse118I	RCCGGY	1 cut(s)	109
Bse1I	ACTGG	9 cut(s)	134, 557, 583, 1148, 1850, 2911, 2921, 3204, 3326
Bse21I	CCTNAGG	1 cut(s)	1616
Bse3DI	GCAATG	1 cut(s)	2921
Bse8I	GATNNNNATC	1 cut(s)	445
BseBI	CCWGG	4 cut(s)	494, 1491, 1928, 3251
BseDI	CCNNGG	5 cut(s)	87, 522, 1926, 2055, 3250
BseJI	GATNNNNATC	1 cut(s)	445
BseLI	CCNNNNNNNGG	6 cut(s)	484, 485, 1252, 1819, 3025, 3256
BseMI	GCAATG	1 cut(s)	2921
BseMII	CTCAG	4 cut(s)	95, 475, 681, 2085
BseNI	ACTGG	9 cut(s)	134, 557, 583, 1148, 1850, 2911, 2921, 3204, 3326
BseRI	GAGGAG	9 cut(s)	6, 56, 155, 620, 1658, 1675, 2604, 2948, 3232
BseXI	GCAGC	1 cut(s)	306
BseYI	CCCAGC	1 cut(s)	479
BsgI	GTGCAG	3 cut(s)	857, 2949, 2994
Bsh1236I	CGCG	1 cut(s)	1416
BshFI	GGCC	5 cut(s)	1375, 1679, 1880, 2020, 2688
BshNI	GGYRCC	1 cut(s)	52
BsiHKAI	GWGCWC	2 cut(s)	1992, 2771
BsiHKCI	CYCGRG	2 cut(s)	1707, 3173
BsiSI	CCGG	3 cut(s)	110, 1555, 2021
BslFI	GGGAC	8 cut(s)	1208, 1571, 1754, 1890, 2315, 2348, 3104, 3156
BslI	CCNNNNNNNGG	6 cut(s)	484, 485, 1252, 1819, 3025, 3256
BsmAI	GTCTC	1 cut(s)	2999
BsmFI	GGGAC	8 cut(s)	1208, 1571, 1754, 1890, 2315, 2348, 3104, 3156
BsmI	GAATGC	2 cut(s)	953, 3152
BsnI	GGCC	5 cut(s)	1375, 1679, 1880, 2020, 2688
BsoBI	CYCGRG	2 cut(s)	1707, 3173
Bsp119I	TTCGAA	1 cut(s)	1786
Bsp1286I	GDGCHC	3 cut(s)	594, 1992, 2771
Bsp1407I	TGTACA	2 cut(s)	1548, 2383
Bsp143I	GATC	9 cut(s)	28, 466, 994, 1216, 1387, 2515, 2862, 2871, 3284
Bsp19I	CCATGG	1 cut(s)	87
BspACI	CCGC	5 cut(s)	56, 244, 305, 1297, 2974
BspANI	GGCC	5 cut(s)	1375, 1679, 1880, 2020, 2688
BspCNI	CTCAG	4 cut(s)	94, 474, 682, 2086
BspFNI	CGCG	1 cut(s)	1416
BspHI	TCATGA	2 cut(s)	2968, 3064
BspLI	GGNNCC	6 cut(s)	54, 108, 144, 1301, 3045, 3120
BspMAI	CTGCAG	1 cut(s)	3186
BspMI	ACCTGC	3 cut(s)	3016, 3106, 3189
BspPI	GGATC	1 cut(s)	2510
BspQI	GCTCTTC	1 cut(s)	732
BspT104I	TTCGAA	1 cut(s)	1786
BspT107I	GGYRCC	1 cut(s)	52
BsrDI	GCAATG	1 cut(s)	2921
BsrFI	RCCGGY	1 cut(s)	109
BsrGI	TGTACA	2 cut(s)	1548, 2383
BsrI	ACTGG	9 cut(s)	134, 557, 583, 1148, 1850, 2911, 2921, 3204, 3326
BssAI	RCCGGY	1 cut(s)	109
BssECI	CCNNGG	5 cut(s)	87, 522, 1926, 2055, 3250
BssMI	GATC	9 cut(s)	28, 466, 994, 1216, 1387, 2515, 2862, 2871, 3284
BssNI	GRCGYC	1 cut(s)	2361
BssSI	CACGAG	1 cut(s)	423
BssT1I	CCWWGG	2 cut(s)	87, 2055
Bst2BI	CACGAG	1 cut(s)	423
Bst2UI	CCWGG	4 cut(s)	494, 1491, 1928, 3251
Bst6I	CTCTTC	7 cut(s)	732, 1239, 1317, 1430, 1464, 2943, 3123
BstACI	GRCGYC	1 cut(s)	2361
BstAPI	GCANNNNNTGC	1 cut(s)	319
BstAUI	TGTACA	2 cut(s)	1548, 2383
BstBAI	YACGTR	1 cut(s)	810
BstBI	TTCGAA	1 cut(s)	1786
BstC8I	GCNNGC	6 cut(s)	111, 315, 1343, 2928, 3097, 3264
BstDEI	CTNAG	8 cut(s)	81, 461, 690, 1116, 1324, 1616, 2094, 3279
BstDSI	CCRYGG	1 cut(s)	87
BstEII	GGTNACC	1 cut(s)	488
BstENI	CCTNNNNNAGG	1 cut(s)	1817
BstFNI	CGCG	1 cut(s)	1416
BstH2I	RGCGCY	1 cut(s)	320
BstHHI	GCGC	3 cut(s)	319, 346, 2542
BstKTI	GATC	9 cut(s)	31, 469, 997, 1219, 1390, 2518, 2865, 2874, 3287
BstMAI	GTCTC	1 cut(s)	2999
BstMBI	GATC	9 cut(s)	28, 466, 994, 1216, 1387, 2515, 2862, 2871, 3284
BstMWI	GCNNNNNNNGC	6 cut(s)	55, 319, 1861, 2189, 2680, 3272
BstNI	CCWGG	4 cut(s)	494, 1491, 1928, 3251
BstNSI	RCATGY	4 cut(s)	220, 703, 1543, 3266
BstPI	GGTNACC	1 cut(s)	488
BstSCI	CCNGG	5 cut(s)	492, 1489, 1554, 1926, 3249
BstSFI	CTRYAG	3 cut(s)	24, 360, 3182
BstSNI	TACGTA	1 cut(s)	810
BstUI	CGCG	1 cut(s)	1416
BstV1I	GCAGC	1 cut(s)	306
BstV2I	GAAGAC	1 cut(s)	2957
BstX2I	RGATCY	2 cut(s)	2862, 2871
BstYI	RGATCY	2 cut(s)	2862, 2871
Bsu36I	CCTNAGG	1 cut(s)	1616
BsuI	GTATCC	3 cut(s)	240, 799, 1839
BsuRI	GGCC	5 cut(s)	1375, 1679, 1880, 2020, 2688
BtgI	CCRYGG	1 cut(s)	87
BtsI	GCAGTG	2 cut(s)	772, 2535
BtsIMutI	CAGTG	9 cut(s)	141, 469, 772, 1397, 1567, 1857, 2535, 2769, 2890
BveI	ACCTGC	3 cut(s)	3016, 3106, 3189
Cac8I	GCNNGC	6 cut(s)	111, 315, 1343, 2928, 3097, 3264
CaiI	CAGNNNCTG	2 cut(s)	319, 3250
CciI	TCATGA	2 cut(s)	2968, 3064
CfoI	GCGC	3 cut(s)	319, 346, 2542
Cfr10I	RCCGGY	1 cut(s)	109
CsiI	ACCWGGT	1 cut(s)	492
DdeI	CTNAG	8 cut(s)	81, 461, 690, 1116, 1324, 1616, 2094, 3279
DpnI	GATC	9 cut(s)	30, 468, 996, 1218, 1389, 2517, 2864, 2873, 3286
DpnII	GATC	9 cut(s)	28, 466, 994, 1216, 1387, 2515, 2862, 2871, 3284
DraIII	CACNNNGTG	1 cut(s)	2885
DriI	GACNNNNNGTC	1 cut(s)	283
Eam1104I	CTCTTC	7 cut(s)	732, 1239, 1317, 1430, 1464, 2943, 3123
Eam1105I	GACNNNNNGTC	1 cut(s)	283
EarI	CTCTTC	7 cut(s)	732, 1239, 1317, 1430, 1464, 2943, 3123
EciI	GGCGGA	2 cut(s)	259, 320
Ecl136II	GAGCTC	1 cut(s)	1990
Eco105I	TACGTA	1 cut(s)	810
Eco130I	CCWWGG	2 cut(s)	87, 2055
Eco147I	AGGCCT	1 cut(s)	2688
Eco24I	GRGCYC	2 cut(s)	594, 1992
Eco47I	GGWCC	7 cut(s)	77, 541, 1717, 1762, 2268, 2617, 3118
Eco53kI	GAGCTC	1 cut(s)	1990
Eco57I	CTGAAG	6 cut(s)	643, 690, 1113, 1139, 1145, 3105
Eco81I	CCTNAGG	1 cut(s)	1616
Eco88I	CYCGRG	2 cut(s)	1707, 3173
Eco91I	GGTNACC	1 cut(s)	488
EcoICRI	GAGCTC	1 cut(s)	1990
EcoNI	CCTNNNNNAGG	1 cut(s)	1817
EcoO109I	RGGNCCY	5 cut(s)	77, 541, 1717, 2268, 3118
EcoO65I	GGTNACC	1 cut(s)	488
EcoRI	GAATTC	1 cut(s)	622
EcoRII	CCWGG	4 cut(s)	492, 1489, 1926, 3249
EcoT14I	CCWWGG	2 cut(s)	87, 2055
EcoT22I	ATGCAT	1 cut(s)	953
EcoT38I	GRGCYC	2 cut(s)	594, 1992
ErhI	CCWWGG	2 cut(s)	87, 2055
FalI	AAGNNNNNCTT	4 cut(s)	703, 735, 1998, 2030
FaqI	GGGAC	8 cut(s)	1208, 1571, 1754, 1890, 2315, 2348, 3104, 3156
FauNDI	CATATG	3 cut(s)	678, 3289, 3316
FbaI	TGATCA	1 cut(s)	1387
Fnu4HI	GCNGC	2 cut(s)	56, 320
FriOI	GRGCYC	2 cut(s)	594, 1992
Fsp4HI	GCNGC	2 cut(s)	56, 320
FspBI	CTAG	1 cut(s)	1721
FspI	TGCGCA	2 cut(s)	345, 2541
GlaI	GCGC	3 cut(s)	318, 345, 2541
GluI	GCNGC	2 cut(s)	56, 320
GsaI	CCCAGC	1 cut(s)	483
GsuI	CTGGAG	3 cut(s)	617, 983, 1131
HaeII	RGCGCY	1 cut(s)	320
HaeIII	GGCC	5 cut(s)	1375, 1679, 1880, 2020, 2688
HapII	CCGG	3 cut(s)	110, 1555, 2021
HhaI	GCGC	3 cut(s)	319, 346, 2542
Hin1I	GRCGYC	1 cut(s)	2361
Hin6I	GCGC	3 cut(s)	317, 344, 2540
HinP1I	GCGC	3 cut(s)	317, 344, 2540
HindIII	AAGCTT	3 cut(s)	746, 866, 2081
HpaII	CCGG	3 cut(s)	110, 1555, 2021
HphI	GGTGA	5 cut(s)	1085, 1266, 1475, 2749, 3083
Hpy99I	CGWCG	1 cut(s)	230
HpyCH4IV	ACGT	3 cut(s)	809, 1063, 2361
HpyF10VI	GCNNNNNNNGC	6 cut(s)	55, 319, 1861, 2189, 2680, 3272
HpyF3I	CTNAG	8 cut(s)	81, 461, 690, 1116, 1324, 1616, 2094, 3279
HpySE526I	ACGT	3 cut(s)	809, 1063, 2361
Hsp92I	GRCGYC	1 cut(s)	2361
HspAI	GCGC	3 cut(s)	317, 344, 2540
KroI	GCCGGC	1 cut(s)	109
KroNI	GCCGGC	1 cut(s)	111
Ksp22I	TGATCA	1 cut(s)	1387
Kzo9I	GATC	9 cut(s)	28, 466, 994, 1216, 1387, 2515, 2862, 2871, 3284
LguI	GCTCTTC	1 cut(s)	732
LmnI	GCTCC	8 cut(s)	106, 142, 605, 2197, 3233, 3241, 3259, 3280
Lsp1109I	GCAGC	1 cut(s)	306
LweI	GCATC	7 cut(s)	36, 723, 960, 1102, 1464, 1973, 3012
MabI	ACCWGGT	1 cut(s)	492
MaeI	CTAG	1 cut(s)	1721
MaeII	ACGT	3 cut(s)	809, 1063, 2361
MaeIII	GTNAC	6 cut(s)	488, 1073, 1272, 1417, 1562, 3308
MalI	GATC	9 cut(s)	30, 468, 996, 1218, 1389, 2517, 2864, 2873, 3286
MboI	GATC	9 cut(s)	28, 466, 994, 1216, 1387, 2515, 2862, 2871, 3284
MfeI	CAATTG	2 cut(s)	171, 1952
MflI	RGATCY	2 cut(s)	2862, 2871
MhlI	GDGCHC	3 cut(s)	594, 1992, 2771
MluI	ACGCGT	1 cut(s)	1414
MlyI	GAGTC	5 cut(s)	7, 557, 992, 2526, 3182
MmeI	TCCRAC	5 cut(s)	173, 311, 1927, 2860, 3237
Mph1103I	ATGCAT	1 cut(s)	953
MroNI	GCCGGC	1 cut(s)	109
MslI	CAYNNNNRTG	7 cut(s)	956, 1335, 1359, 1544, 2469, 3069, 3315
MspI	CCGG	3 cut(s)	110, 1555, 2021
MspR9I	CCNGG	5 cut(s)	494, 1491, 1556, 1928, 3251
MunI	CAATTG	2 cut(s)	171, 1952
Mva1269I	GAATGC	2 cut(s)	953, 3152
MvaI	CCWGG	4 cut(s)	494, 1491, 1928, 3251
MvnI	CGCG	1 cut(s)	1416
MwoI	GCNNNNNNNGC	6 cut(s)	55, 319, 1861, 2189, 2680, 3272
NaeI	GCCGGC	1 cut(s)	111
NciI	CCSGG	1 cut(s)	1556
NcoI	CCATGG	1 cut(s)	87
NdeI	CATATG	3 cut(s)	678, 3289, 3316
NdeII	GATC	9 cut(s)	28, 466, 994, 1216, 1387, 2515, 2862, 2871, 3284
NgoMIV	GCCGGC	1 cut(s)	109
NlaIV	GGNNCC	6 cut(s)	54, 108, 144, 1301, 3045, 3120
NmeAIII	GCCGAG	1 cut(s)	547
NmuCI	GTSAC	5 cut(s)	1073, 1272, 1417, 1562, 3308
NsbI	TGCGCA	2 cut(s)	345, 2541
NsiI	ATGCAT	1 cut(s)	953
NspI	RCATGY	4 cut(s)	220, 703, 1543, 3266
NspV	TTCGAA	1 cut(s)	1786
PaeI	GCATGC	1 cut(s)	3266
PaeR7I	CTCGAG	1 cut(s)	1707
PagI	TCATGA	2 cut(s)	2968, 3064
PceI	AGGCCT	1 cut(s)	2688
PciI	ACATGT	1 cut(s)	216
PciSI	GCTCTTC	1 cut(s)	732
PcsI	WCGNNNNNNNCGW	3 cut(s)	815, 1783, 1909
PctI	GAATGC	2 cut(s)	953, 3152
PdiI	GCCGGC	1 cut(s)	111
PfoI	TCCNGGA	1 cut(s)	1554
PkrI	GCNGC	2 cut(s)	57, 321
PleI	GAGTC	5 cut(s)	7, 557, 991, 2526, 3182
PpsI	GAGTC	5 cut(s)	7, 557, 991, 2526, 3182
Ppu21I	YACGTR	1 cut(s)	810
PpuMI	RGGWCCY	5 cut(s)	77, 541, 1717, 2268, 3118
PscI	ACATGT	1 cut(s)	216
PshBI	ATTAAT	2 cut(s)	2172, 2787
PsiI	TTATAA	1 cut(s)	237
Psp124BI	GAGCTC	1 cut(s)	1992
Psp5II	RGGWCCY	5 cut(s)	77, 541, 1717, 2268, 3118
Psp6I	CCWGG	4 cut(s)	492, 1489, 1926, 3249
PspEI	GGTNACC	1 cut(s)	488
PspFI	CCCAGC	1 cut(s)	479
PspGI	CCWGG	4 cut(s)	492, 1489, 1926, 3249
PspN4I	GGNNCC	6 cut(s)	54, 108, 144, 1301, 3045, 3120
PspPPI	RGGWCCY	5 cut(s)	77, 541, 1717, 2268, 3118
PstI	CTGCAG	1 cut(s)	3186
PstNI	CAGNNNCTG	2 cut(s)	319, 3250
PsuI	RGATCY	2 cut(s)	2862, 2871
RseI	CAYNNNNRTG	7 cut(s)	956, 1335, 1359, 1544, 2469, 3069, 3315
SacI	GAGCTC	1 cut(s)	1992
SapI	GCTCTTC	1 cut(s)	732
SatI	GCNGC	2 cut(s)	56, 320
Sau3AI	GATC	9 cut(s)	28, 466, 994, 1216, 1387, 2515, 2862, 2871, 3284
SbfI	CCTGCAGG	1 cut(s)	3186
ScaI	AGTACT	2 cut(s)	386, 2915
SchI	GAGTC	5 cut(s)	7, 557, 992, 2526, 3182
ScrFI	CCNGG	5 cut(s)	494, 1491, 1556, 1928, 3251
SdaI	CCTGCAGG	1 cut(s)	3186
SduI	GDGCHC	3 cut(s)	594, 1992, 2771
SexAI	ACCWGGT	1 cut(s)	492
SfaNI	GCATC	7 cut(s)	36, 723, 960, 1102, 1464, 1973, 3012
SfcI	CTRYAG	3 cut(s)	24, 360, 3182
Sfr274I	CTCGAG	1 cut(s)	1707
SfuI	TTCGAA	1 cut(s)	1786
SinI	GGWCC	7 cut(s)	77, 541, 1717, 1762, 2268, 2617, 3118
SlaI	CTCGAG	1 cut(s)	1707
SmiMI	CAYNNNNRTG	7 cut(s)	956, 1335, 1359, 1544, 2469, 3069, 3315
SmlI	CTYRAG	7 cut(s)	99, 593, 869, 1067, 1707, 1991, 2843
SmoI	CTYRAG	7 cut(s)	99, 593, 869, 1067, 1707, 1991, 2843
SnaBI	TACGTA	1 cut(s)	810
SphI	GCATGC	1 cut(s)	3266
Sse8387I	CCTGCAGG	1 cut(s)	3186
SseBI	AGGCCT	1 cut(s)	2688
SsiI	CCGC	5 cut(s)	56, 244, 305, 1297, 2974
SspMI	CTAG	1 cut(s)	1721
SstI	GAGCTC	1 cut(s)	1992
StuI	AGGCCT	1 cut(s)	2688
StyD4I	CCNGG	5 cut(s)	492, 1489, 1554, 1926, 3249
StyI	CCWWGG	2 cut(s)	87, 2055
TaiI	ACGT	3 cut(s)	812, 1066, 2364
TaqI	TCGA	7 cut(s)	72, 185, 1529, 1708, 1786, 2278, 2587
TatI	WGTACW	5 cut(s)	116, 384, 1548, 2383, 2913
TauI	GCSGC	1 cut(s)	58
TscAI	CASTG	9 cut(s)	141, 469, 772, 1397, 1567, 1857, 2542, 2776, 2890
TseFI	GTSAC	5 cut(s)	1073, 1272, 1417, 1562, 3308
TseI	GCWGC	1 cut(s)	319
Tsp45I	GTSAC	5 cut(s)	1073, 1272, 1417, 1562, 3308
TspGWI	ACGGA	2 cut(s)	77, 524
TspRI	CASTG	9 cut(s)	141, 469, 772, 1397, 1567, 1857, 2542, 2776, 2890
VpaK11BI	GGWCC	7 cut(s)	77, 541, 1717, 1762, 2268, 2617, 3118
VspI	ATTAAT	2 cut(s)	2172, 2787
XagI	CCTNNNNNAGG	1 cut(s)	1817
XapI	RAATTY	4 cut(s)	622, 1226, 2028, 3034
XceI	RCATGY	4 cut(s)	220, 703, 1543, 3266
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1487
XhoI	CTCGAG	1 cut(s)	1707
XspI	CTAG	1 cut(s)	1721
ZraI	GACGTC	1 cut(s)	2362
ZrmI	AGTACT	2 cut(s)	386, 2915
Zsp2I	ATGCAT	1 cut(s)	953

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.