RH5DG347800

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0000333
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG347800.1

Sequence Viewer

Length: 3357 bp
ATGACTGTCATGACCCCTGCCCCTATTGATCAACCAGAGGACGAGGAGATGCTTGTTCCAGCCTCGGATTTGCCTGAAAACAATCACCAGCCCATGGATGTTGTTGCACAGCCTGAAAATGCTAATAATGTGGAGAACCAACCAGTAGAAGATCCTCCTTCATCGAGATTCACATGGAGAATTGACAACTTTTCTAGGATGAATACAAAGAAGCTTTATTCTGACATATTTGTTGTTGGTGGCTATAAATGGCGTGTGCTTATTTTTCCCAAGGGAAACAATGTGGATCATTTGTCTATGTATTTGGATGTTGCGGATTCGTCAAATTTGCCATATGGGTGGAGTAGATATGCTCAATTTAGCTTGGGAGTTCTCAATCAACTTCATACCAAGTATTCTGTACGAAAAGACACACAACATCAGTTCAATGCACGGGAAAGTGATTGGGGTTTCACATCCTTTATGCCTCTTGGTGAACTGTACGATCCCAGCAGAGGTTATCTCGTGAATGATGCGGTTATAATTGAAGCGGAAGTTCTCGTTCGTAGGGTTGTAGATTATTGGACATATGACTCAAAGAAGGAAACGGGGTATGTTGGGCTTAAGAACCAAGGAGCAACATGCTATATGAACTCTCTCCTGCAGACTCTGTATCATATTCCTTATTTTAGAAAGGCTGTTTACCATATGCCCACGACTGAGAATGATTTGCCATCACAAAGCATACCATTGGCTCTTCAGAGTCTATTTTACAAACTCCAGTACAGTGATAATAGTGTTGCAACAAAAGAGCTGACAAAGTCTTTTGGATGGGATACATATGATTCATTTATGCAGCATGATGTGCAAGAACTAAATAGGGTCTTGTGTGAAAAACTGGAGGACAAAATGAAGGGAACTGTTGTAGAGGGAACCATACAGAAGTTATTTGAAGGTCACCATATGAACTACATCGAATGCATTAATGTGGACTACAAATCTACAAGAAAGGAGTCATTTTATGACCTTCAGCTCGATGTCAAAGGGTGTCGTGATGTATATGCTTCTTTTGATAAGTATGTAGAAGTTGAACGTCTTGAGGGGGATAACAAGTATCATGCTGAACAGAATGGATTACAGGATGCTAAGAAAGGGGTTCTCTTCATTGACTTTCCACCTGTTCTTCAACTTCAACTGAAACGGTTTGAGTACGACTTTATGCGAGACACCATGGTGAAGATTAATGACCGTTATGAGTTCCCTTTGCAACTTGACCTTGATCGAGATAATGGAAAATATTTATCACCTGAAGCTGATAGGAGTGTTCGCAACCTTTACACACTTCACAGTGTATTGGTACACAGTGGTGGGGTGCATGGTGGACATTACTACGCCTTTATCAGACCGACGCTTTCTGATCAGTGGTACAAATTTGATGATGAGAGGGTAACAAAAGAAGATATAAAGAGGGCACTGGAGGAGCAGTATGGTGGTGAGGAAGAGTTACCGCAGACAAACCCTGGATTTAATAACACTCCTTTCAAATTTACAAAATACTCAAACGCGTACATGCTGGTGTACATACGTGAAAGTGACAAGGATAAAATAATCTGCAATGTTGATGAGAAGGACATTGCTGAACATCTTCGGGAGAGGTTGAAGAAAGAACAAGAAGAAAAGGAGCACAAGAAGAAAGAAAAGGCAGAGGCACACCTATATACTATCATTAAGGTTGCCCGGGACGAGAACCTTCTGGAGCAGATTGGGAAGGATATATATTTTGATCTTGTGGATCATGACAAAGTCAAAAGTTTTCGTATTCAGAAGCAGATGCCTTTCAACCTTTTCAAGGAGGAGGTTGCTAAGGAGTTTGGCATACCAGTGCAATTTCAGCGGTTTTGGCTGTGGGCAAAGCGTCAAAACCATACATATCGTCCAAACCGTCCATTGACACCTGTGGAGGAAACACAATCTGTTGGGCAGTTGAGGGAGGTATCAAACAAGGTTCATAATGCAGAACTGAAGTTGTTCTTGGAAATAGAGCTTGGACCGGATTTACACCCTATTGCTCCACCTGACAAGACAAAGGATGATATTCTGCTTTTCTTTAAGCTTTATGATCCTGAAAAAGAAGAACTACGTTATGTTGGACGGCTTTTTGTGAAGAGTACTGGTAAGCCAGCTGAAATCTTGTCAAAATTAAATGAATTGGCTGGCTATGCCCCTGATGAGGAGATCGACCTTTACGAGGAAATTAAGTATGAGCCCACTGTCATGTGTGAACCCATCGATAAGAAATTCACATTTCGAGCCAGCCAGCTTGAGGATGGAGACATTGTTTGCTTCCAAAAACCAGTTCCGGTTGAAAATGGTCAGCAGTTCCGCTATCCAGACGTCCCATCTTTTCTGGATTTTGTGCACAATCGTCTCGTTGTTCATTTTCGATCGTTTGAGAAACCCAAAGAAGATGATTTCAGCTTAGAATTGTCAAAACTTCATACCTATGATGATGTTGTGGAGCGAGTAGCTCAACAACTTGGTTTGGACGATCCATCCAAAATTAGGCTTACCTCACATAATTGTTACTCCCAGCAACCCAAACCTCAGCCAATTAAGTATCGGGGGGTAGACCGTTTGACTGATATGCTTGTTCACTACAATCAGACTTCGGATATATTGTACTATGAAGTATTAGACATCCCTCTGCCTGAATTACAAGGTTTGAAAACCCTGAAAGTTGCATTCCATCATGCTACCAAGGATGAAGTGGTTATTCACACCATAAGACTGCCAAAACAGAGCACTGTTGGGGATGTAATCAATGATCTTAAAACTAAGGTTGAATTGTCTCATCCAGATGCAGAACTTAGGTTGCTCGAGGTTTTCTATCACAAGATATACAAGGTCTTTCCCCAAAGTGAAAAGATCGAAAACATAAATGATCAATACTGGACGTTACGTGCTGAAGAGGTTCCAGAGGAAGAGAAAAATCTTGGTCCTAATGACCGTGTAATTCATGTCTACCACTTCACTAAAGACACCGCTCAGAATCAAATGCAAATTCAAAACTTTGGGGAACCGTTTTTCTTGGTTATACATGAAGGTGAGACCTTGGCTGAAATTAGAGGTCGGATACAAAAGAAATTACAGGTTGCAGATGAGGAATTTGCGAAGTGGAAGTTTGCATTTCTTTCGCTAGGTCGGCCAGAGTATCTTCAGGACACTGACATCGTCTCTAGTCGGTTCCAGAGAAGAGATGTTTATGGTGCTTGGGAGCAGTATCTTGGCCTGGAGCACTCTGACAGCGCTCCTAAAAGAGCTTATGCAGCTAATCAGAATCGCCACACATTTGAGAAGCCGGTGAAAATTTACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko05168 ko05169 ko05203 map04068 map05168 map05169 map05203
Pfam Domains
Protein Families
MATH UCH USP7_C2 USP7_ICP0_bdg

Protein Analysis

1118

Amino Acids

131.0

Weight (kDa)

5.52

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MATH_2 PF22486 56 - 179 5.2e-39 MATH domain
MATH PF00917 61 - 180 1.5e-17 MATH domain
UCH PF00443 199 - 520 1.6e-59 Ubiquitin carboxyl-terminal hydrolase
UCH_1 PF13423 199 - 474 2e-15 Ubiquitin carboxyl-terminal hydrolase
USP7_ICP0_bdg PF12436 624 - 876 3.9e-78 ICP0-binding domain of Ubiquitin-specific protease 7
USP7_C2 PF14533 886 - 1096 1.1e-55 Ubiquitin-specific protease C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000333)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11910 AT3G11910 AT3G11910 AT3G11910 AT5G06600 AT5G06600 AT5G06600
fragaria_vesca FvH4_3g26930 FvH4_3g39132 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220 FvH4_6g21220
malus_domestica MD02G1261100.v1.1 MD03G1161100.v1.1 MD07G1059900.v1.1 MD11G1177100.v1.1 MD12G1047900.v1.1 MD12G1048000.v1.1 MD12G1048400.v1.1 MD12G1048500.v1.1 MD12G1048700.v1.1 MD12G1048800.v1.1 MD12G1056200.v1.1 MD14G1046300.v1.1 MD14G1046500.v1.1 MD15G1166900.v1.1
prunus_persica Prupe.2G066200_v2.0.a1 Prupe.2G066200_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.6G149500_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1 Prupe.7G087600_v2.0.a1
pyrus_communis pycom02g22320 pycom03g11340 pycom07g04580 pycom11g14290 pycom11g15110 pycom12g04110 pycom12g04140 pycom12g04160 pycom12g04210 pycom12g04220 pycom12g04230 pycom12g04290 pycom12g04320 pycom12g04330 pycom12g04350 pycom12g04360 pycom14g03880 pycom14g03900 pycom16g18750
rosa_chinensis RchiOBHm_Chr1g0324111 RchiOBHm_Chr1g0324241 RchiOBHm_Chr3g0477691 RchiOBHm_Chr5g0049371 RchiOBHm_Chr5g0070531
rosa_laevigata RLG00000008612 RLG00000023698 RLG00000034613 RLG00000036139
rosa_multiflora Rmu_co8271779.1_g000001 Rmu_sc0006187.1_g000014
rosa_roxburghii Rroxscaffold_1G00010670 Rroxscaffold_1G00030450 Rroxscaffold_1G00031950 Rroxscaffold_1G00031970 Rroxscaffold_1G00032320 Rroxscaffold_1G00055490 Rroxscaffold_2G00083610 Rroxscaffold_2G00084480 Rroxscaffold_2G00115610 Rroxscaffold_3G00261320 Rroxscaffold_4G00312220 Rroxscaffold_4G00325820 Rroxscaffold_5G00339170 Rroxscaffold_5G00364030 Rroxscaffold_6G00403750
rosa_rugosa Rorug03G0162700 Rorug03G0162800 Rorug03G0162900 Rorug05G0247700 Rorug05G0406600 Rorug05G0406800
rosa_samantha Rh1AG060300 Rh3AG213000 Rh3BG246600 Rh3CG240400 Rh3DG239700 Rh5AG324400 Rh5AG462100 Rh5BG335500 Rh5BG480200 Rh5BG480300 Rh5CG360200 Rh5CG504500 Rh5CG504600 Rh5DG347800 Rh5DG492200 Rh5DG492300
rosa_wichuraiana Rw3G019270 Rw3G026910 Rw5G030650 Rw5G032610 Rw5G043040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 521
AatII GACGTC 1 cut(s) 2376
AccB7I CCANNNNNTGG 1 cut(s) 94
AccBSI CCGCTC 1 cut(s) 3023
AccI GTMKAC 2 cut(s) 2607, 3000
AccII CGCG 1 cut(s) 1544
AciI CCGC 7 cut(s) 314, 515, 530, 1487, 1873, 2362, 3021
AclWI GGATC 6 cut(s) 146, 294, 479, 1779, 2093, 2522
AcoI YGGCCR 1 cut(s) 3182
AcsI RAATTY 7 cut(s) 325, 1409, 1523, 2276, 3039, 3143, 3345
AcuI CTGAAG 6 cut(s) 722, 992, 1308, 2021, 2964, 3179
AcyI GRCGYC 1 cut(s) 2373
AfeI AGCGCT 1 cut(s) 3286
AfiI CCNNNNNNNGG 7 cut(s) 94, 494, 1828, 2209, 2227, 2302, 2541
AflII CTTAAG 1 cut(s) 602
AflIII ACRYGT 1 cut(s) 1542
AjnI CCWGG 2 cut(s) 1498, 3267
AjuI GAANNNNNNNTTGG 6 cut(s) 1994, 2007, 2026, 2039, 2319, 2351
AleI CACNNNNGTG 2 cut(s) 1211, 1344
Alw21I GWGCWC 4 cut(s) 1665, 2400, 2783, 3276
Alw26I GTCTC 6 cut(s) 1197, 2304, 2411, 2832, 3080, 3217
Alw44I GTGCAC 1 cut(s) 2396
AlwI GGATC 6 cut(s) 146, 294, 479, 1779, 2093, 2522
AlwNI CAGNNNCTG 1 cut(s) 649
Ama87I CYCGRG 2 cut(s) 1716, 2855
Aor51HI AGCGCT 1 cut(s) 3286
AoxI GGCC 2 cut(s) 3182, 3265
ApaLI GTGCAC 1 cut(s) 2396
ApeKI GCWGC 2 cut(s) 835, 3305
ApoI RAATTY 7 cut(s) 325, 1409, 1523, 2276, 3039, 3143, 3345
AseI ATTAAT 2 cut(s) 963, 1221
Asp700I GAANNNNTTC 3 cut(s) 1623, 2006, 2949
AspLEI GCGC 1 cut(s) 3287
AspS9I GGNCC 2 cut(s) 2027, 2975
AsuC2I CCSGG 2 cut(s) 1717, 1718
AsuHPI GGTGA 8 cut(s) 77, 485, 929, 1225, 1275, 1484, 3095, 3352
AvaI CYCGRG 2 cut(s) 1716, 2855
AvaII GGWCC 2 cut(s) 2027, 2975
BaeGI GKGCMC 2 cut(s) 1453, 2400
BanII GRGCYC 1 cut(s) 2247
BauI CACGAG 1 cut(s) 503
Bbv12I GWGCWC 4 cut(s) 1665, 2400, 2783, 3276
BbvCI CCTCAGC 1 cut(s) 2583
BbvI GCAGC 2 cut(s) 847, 3317
BccI CCATC 7 cut(s) 721, 804, 2273, 2300, 2386, 2539, 2733
BceAI ACGGC 1 cut(s) 2147
BcgI CGANNNNNNTGC 2 cut(s) 3153, 3187
BciT130I CCWGG 2 cut(s) 1500, 3269
BciVI GTATCC 2 cut(s) 808, 3105
BclI TGATCA 3 cut(s) 28, 1396, 2920
BcnI CCSGG 2 cut(s) 1717, 1718
BcoDI GTCTC 6 cut(s) 1197, 2304, 2411, 2832, 3080, 3217
BfaI CTAG 3 cut(s) 195, 3176, 3216
BfmI CTRYAG 1 cut(s) 641
BfoI RGCGCY 1 cut(s) 3288
BfrI CTTAAG 1 cut(s) 602
BfuI GTATCC 2 cut(s) 808, 3105
BisI GCNGC 2 cut(s) 836, 3306
BlsI GCNGC 2 cut(s) 837, 3307
BmcAI AGTACT 1 cut(s) 2149
Bme1390I CCNGG 4 cut(s) 1500, 1717, 1718, 3269
Bme18I GGWCC 2 cut(s) 2027, 2975
BmeT110I CYCGRG 2 cut(s) 1716, 2855
BmgT120I GGNCC 2 cut(s) 2027, 2975
BmiI GGNNCC 4 cut(s) 913, 2952, 3057, 3224
BmrFI CCNGG 4 cut(s) 1500, 1717, 1718, 3269
BmsI GCATC 5 cut(s) 39, 502, 1111, 1800, 2827
BplI GAGNNNNNCTC 2 cut(s) 486, 518
BpmI CTGGAG 5 cut(s) 743, 899, 1475, 1754, 3290
Bpu10I CCTNAGC 2 cut(s) 1842, 2583
BpuEI CTTGAG 2 cut(s) 1097, 2321
BpuMI CCSGG 2 cut(s) 1717, 1718
Bsa29I ATCGAT 1 cut(s) 2268
BsaAI YACGTR 2 cut(s) 1565, 2939
BsaHI GRCGYC 1 cut(s) 2373
BsaI GGTCTC 1 cut(s) 3080
BsaJI CCNNGG 9 cut(s) 63, 93, 270, 610, 1209, 1498, 1716, 2736, 3090
BsaWI WCCGGW 2 cut(s) 2029, 2338
BsaXI ACNNNNNCTCC 4 cut(s) 1448, 1478, 2301, 2331
Bsc4I CCNNNNNNNGG 7 cut(s) 94, 494, 1828, 2209, 2227, 2302, 2541
Bse118I RCCGGY 1 cut(s) 3337
Bse1I ACTGG 8 cut(s) 143, 760, 882, 1458, 1859, 2155, 2333, 2933
Bse3DI GCAATG 2 cut(s) 1600, 1611
BseBI CCWGG 2 cut(s) 1500, 3269
BseCI ATCGAT 1 cut(s) 2268
BseDI CCNNGG 9 cut(s) 63, 93, 270, 610, 1209, 1498, 1716, 2736, 3090
BseLI CCNNNNNNNGG 7 cut(s) 94, 494, 1828, 2209, 2227, 2302, 2541
BseMI GCAATG 2 cut(s) 1600, 1611
BseMII CTCAG 3 cut(s) 690, 2597, 3038
BseNI ACTGG 8 cut(s) 143, 760, 882, 1458, 1859, 2155, 2333, 2933
BseRI GAGGAG 4 cut(s) 59, 1472, 1847, 2225
BseSI GKGCMC 2 cut(s) 1453, 2400
BseXI GCAGC 2 cut(s) 847, 3317
BseYI CCCAGC 2 cut(s) 488, 2568
Bsh1236I CGCG 1 cut(s) 1544
Bsh1285I CGRYCG 1 cut(s) 2426
BshFI GGCC 2 cut(s) 3184, 3267
BshVI ATCGAT 1 cut(s) 2268
BsiEI CGRYCG 1 cut(s) 2426
BsiHKAI GWGCWC 4 cut(s) 1665, 2400, 2783, 3276
BsiHKCI CYCGRG 2 cut(s) 1716, 2855
BsiSI CCGG 4 cut(s) 1717, 2030, 2339, 3338
BslFI GGGAC 2 cut(s) 1733, 2360
BslI CCNNNNNNNGG 7 cut(s) 94, 494, 1828, 2209, 2227, 2302, 2541
BsmAI GTCTC 6 cut(s) 1197, 2304, 2411, 2832, 3080, 3217
BsmBI CGTCTC 2 cut(s) 2411, 3217
BsmFI GGGAC 2 cut(s) 1733, 2360
BsmI GAATGC 2 cut(s) 962, 2720
BsnI GGCC 2 cut(s) 3184, 3267
Bso31I GGTCTC 1 cut(s) 3080
BsoBI CYCGRG 2 cut(s) 1716, 2855
Bsp1286I GDGCHC 6 cut(s) 1453, 1665, 2247, 2400, 2783, 3276
Bsp1407I TGTACA 1 cut(s) 1557
Bsp19I CCATGG 2 cut(s) 93, 1209
BspACI CCGC 7 cut(s) 314, 515, 530, 1487, 1873, 2362, 3021
BspANI GGCC 2 cut(s) 3184, 3267
BspCNI CTCAG 3 cut(s) 691, 2596, 3037
BspDI ATCGAT 1 cut(s) 2268
BspFNI CGCG 1 cut(s) 1544
BspHI TCATGA 2 cut(s) 9, 1774
BspLI GGNNCC 4 cut(s) 913, 2952, 3057, 3224
BspMAI CTGCAG 1 cut(s) 645
BspPI GGATC 6 cut(s) 146, 294, 479, 1779, 2093, 2522
BspQI GCTCTTC 1 cut(s) 741
BspTI CTTAAG 1 cut(s) 602
BspTNI GGTCTC 1 cut(s) 3080
BsrBI CCGCTC 1 cut(s) 3023
BsrDI GCAATG 2 cut(s) 1600, 1611
BsrFI RCCGGY 1 cut(s) 3337
BsrGI TGTACA 1 cut(s) 1557
BsrI ACTGG 8 cut(s) 143, 760, 882, 1458, 1859, 2155, 2333, 2933
BssAI RCCGGY 1 cut(s) 3337
BssECI CCNNGG 9 cut(s) 63, 93, 270, 610, 1209, 1498, 1716, 2736, 3090
BssNI GRCGYC 1 cut(s) 2373
BssSI CACGAG 1 cut(s) 503
BssT1I CCWWGG 6 cut(s) 93, 270, 610, 1209, 2736, 3090
Bst2BI CACGAG 1 cut(s) 503
Bst2UI CCWGG 2 cut(s) 1500, 3269
Bst6I CTCTTC 7 cut(s) 741, 1145, 1473, 2138, 2940, 2955, 3226
BstACI GRCGYC 1 cut(s) 2373
BstAFI CTTAAG 1 cut(s) 602
BstAPI GCANNNNNTGC 1 cut(s) 844
BstAUI TGTACA 1 cut(s) 1557
BstBAI YACGTR 2 cut(s) 1565, 2939
BstC8I GCNNGC 4 cut(s) 2160, 2194, 2293, 2297
BstDEI CTNAG 8 cut(s) 699, 1125, 1842, 2458, 2583, 2814, 2846, 3024
BstDSI CCRYGG 2 cut(s) 93, 1209
BstEII GGTNACC 1 cut(s) 935
BstENI CCTNNNNNAGG 2 cut(s) 1826, 2225
BstFNI CGCG 1 cut(s) 1544
BstH2I RGCGCY 1 cut(s) 3288
BstHHI GCGC 1 cut(s) 3287
BstMAI GTCTC 6 cut(s) 1197, 2304, 2411, 2832, 3080, 3217
BstMCI CGRYCG 1 cut(s) 2426
BstMWI GCNNNNNNNGC 6 cut(s) 844, 1870, 1879, 2198, 3181, 3305
BstNI CCWGG 2 cut(s) 1500, 3269
BstNSI RCATGY 2 cut(s) 624, 1552
BstPI GGTNACC 1 cut(s) 935
BstSCI CCNGG 4 cut(s) 1498, 1715, 1716, 3267
BstSFI CTRYAG 1 cut(s) 641
BstSLI GKGCMC 2 cut(s) 1453, 2400
BstUI CGCG 1 cut(s) 1544
BstV1I GCAGC 2 cut(s) 847, 3317
BstX2I RGATCY 1 cut(s) 151
BstXI CCANNNNNNTGG 1 cut(s) 339
BstYI RGATCY 1 cut(s) 151
Bsu15I ATCGAT 1 cut(s) 2268
BsuI GTATCC 2 cut(s) 808, 3105
BsuRI GGCC 2 cut(s) 3184, 3267
BsuTUI ATCGAT 1 cut(s) 2268
BtgI CCRYGG 2 cut(s) 93, 1209
BtsIMutI CAGTG 9 cut(s) 772, 1333, 1348, 1406, 1451, 1866, 2247, 2781, 3201
Cac8I GCNNGC 4 cut(s) 2160, 2194, 2293, 2297
CaiI CAGNNNCTG 1 cut(s) 649
CciI TCATGA 2 cut(s) 9, 1774
CfoI GCGC 1 cut(s) 3287
Cfr10I RCCGGY 1 cut(s) 3337
Cfr13I GGNCC 2 cut(s) 2027, 2975
Cfr9I CCCGGG 1 cut(s) 1716
ClaI ATCGAT 1 cut(s) 2268
CseI GACGC 2 cut(s) 1396, 1883
DdeI CTNAG 8 cut(s) 699, 1125, 1842, 2458, 2583, 2814, 2846, 3024
EaeI YGGCCR 1 cut(s) 3182
Eam1104I CTCTTC 7 cut(s) 741, 1145, 1473, 2138, 2940, 2955, 3226
EarI CTCTTC 7 cut(s) 741, 1145, 1473, 2138, 2940, 2955, 3226
Eco130I CCWWGG 6 cut(s) 93, 270, 610, 1209, 2736, 3090
Eco24I GRGCYC 1 cut(s) 2247
Eco31I GGTCTC 1 cut(s) 3080
Eco47I GGWCC 2 cut(s) 2027, 2975
Eco47III AGCGCT 1 cut(s) 3286
Eco57I CTGAAG 6 cut(s) 722, 992, 1308, 2021, 2964, 3179
Eco88I CYCGRG 2 cut(s) 1716, 2855
Eco91I GGTNACC 1 cut(s) 935
EcoNI CCTNNNNNAGG 2 cut(s) 1826, 2225
EcoO65I GGTNACC 1 cut(s) 935
EcoRII CCWGG 2 cut(s) 1498, 3267
EcoT14I CCWWGG 6 cut(s) 93, 270, 610, 1209, 2736, 3090
EcoT22I ATGCAT 1 cut(s) 962
EcoT38I GRGCYC 1 cut(s) 2247
ErhI CCWWGG 6 cut(s) 93, 270, 610, 1209, 2736, 3090
Esp3I CGTCTC 2 cut(s) 2411, 3217
FalI AAGNNNNNCTT 2 cut(s) 1994, 2026
FaqI GGGAC 2 cut(s) 1733, 2360
FauNDI CATATG 5 cut(s) 334, 568, 687, 820, 942
FbaI TGATCA 3 cut(s) 28, 1396, 2920
FblI GTMKAC 2 cut(s) 2607, 3000
Fnu4HI GCNGC 2 cut(s) 836, 3306
FriOI GRGCYC 1 cut(s) 2247
Fsp4HI GCNGC 2 cut(s) 836, 3306
FspBI CTAG 3 cut(s) 195, 3176, 3216
GlaI GCGC 1 cut(s) 3286
GluI GCNGC 2 cut(s) 836, 3306
GsaI CCCAGC 2 cut(s) 492, 2572
GsuI CTGGAG 5 cut(s) 743, 899, 1475, 1754, 3290
HaeII RGCGCY 1 cut(s) 3288
HaeIII GGCC 2 cut(s) 3184, 3267
HapII CCGG 4 cut(s) 1717, 2030, 2339, 3338
HgaI GACGC 2 cut(s) 1396, 1883
HhaI GCGC 1 cut(s) 3287
Hin1I GRCGYC 1 cut(s) 2373
Hin6I GCGC 1 cut(s) 3285
HinP1I GCGC 1 cut(s) 3285
HindIII AAGCTT 2 cut(s) 212, 2090
HinfI GANTC 9 cut(s) 168, 317, 572, 646, 742, 824, 992, 3028, 3316
HpaII CCGG 4 cut(s) 1717, 2030, 2339, 3338
HphI GGTGA 8 cut(s) 77, 485, 929, 1225, 1275, 1484, 3095, 3352
Hpy99I CGWCG 1 cut(s) 1390
HpyAV CCTTC 9 cut(s) 168, 574, 886, 926, 1016, 1600, 1739, 1741, 3074
HpyCH4IV ACGT 6 cut(s) 1072, 1564, 2119, 2373, 2933, 2938
HpyF10VI GCNNNNNNNGC 6 cut(s) 844, 1870, 1879, 2198, 3181, 3305
HpyF3I CTNAG 8 cut(s) 699, 1125, 1842, 2458, 2583, 2814, 2846, 3024
HpySE526I ACGT 6 cut(s) 1072, 1564, 2119, 2373, 2933, 2938
Hsp92I GRCGYC 1 cut(s) 2373
HspAI GCGC 1 cut(s) 3285
Ksp22I TGATCA 3 cut(s) 28, 1396, 2920
LguI GCTCTTC 1 cut(s) 741
LmnI GCTCC 9 cut(s) 614, 1459, 1660, 1735, 2053, 2497, 3253, 3271, 3292
Lsp1109I GCAGC 2 cut(s) 847, 3317
LweI GCATC 5 cut(s) 39, 502, 1111, 1800, 2827
MaeI CTAG 3 cut(s) 195, 3176, 3216
MaeII ACGT 6 cut(s) 1072, 1564, 2119, 2373, 2933, 2938
MaeIII GTNAC 6 cut(s) 935, 1426, 1482, 1571, 2561, 2934
MbiI CCGCTC 1 cut(s) 3023
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 6 cut(s) 1453, 1665, 2247, 2400, 2783, 3276
MluI ACGCGT 1 cut(s) 1542
MlyI GAGTC 4 cut(s) 566, 640, 751, 1001
MmeI TCCRAC 2 cut(s) 2107, 3089
Mph1103I ATGCAT 1 cut(s) 962
MroXI GAANNNNTTC 3 cut(s) 1623, 2006, 2949
MspA1I CMGCKG 2 cut(s) 1873, 2162
MspCI CTTAAG 1 cut(s) 602
MspI CCGG 4 cut(s) 1717, 2030, 2339, 3338
MspR9I CCNGG 4 cut(s) 1500, 1717, 1718, 3269
Mva1269I GAATGC 2 cut(s) 962, 2720
MvaI CCWGG 2 cut(s) 1500, 3269
MvnI CGCG 1 cut(s) 1544
MwoI GCNNNNNNNGC 6 cut(s) 844, 1870, 1879, 2198, 3181, 3305
NciI CCSGG 2 cut(s) 1717, 1718
NcoI CCATGG 2 cut(s) 93, 1209
NdeI CATATG 5 cut(s) 334, 568, 687, 820, 942
NlaIV GGNNCC 4 cut(s) 913, 2952, 3057, 3224
NmuCI GTSAC 2 cut(s) 935, 1571
NsiI ATGCAT 1 cut(s) 962
NspI RCATGY 2 cut(s) 624, 1552
OliI CACNNNNGTG 2 cut(s) 1211, 1344
PaeR7I CTCGAG 1 cut(s) 2855
PagI TCATGA 2 cut(s) 9, 1774
PciSI GCTCTTC 1 cut(s) 741
PcsI WCGNNNNNNNCGW 2 cut(s) 1918, 2223
PctI GAATGC 2 cut(s) 962, 2720
PdmI GAANNNNTTC 3 cut(s) 1623, 2006, 2949
PfeI GAWTC 5 cut(s) 168, 317, 824, 3028, 3316
PflFI GACNNNGTC 3 cut(s) 799, 1781, 3209
PflMI CCANNNNNTGG 1 cut(s) 94
PkrI GCNGC 2 cut(s) 837, 3307
Ple19I CGATCG 1 cut(s) 2426
PleI GAGTC 4 cut(s) 566, 640, 750, 1000
PpsI GAGTC 4 cut(s) 566, 640, 750, 1000
Ppu21I YACGTR 2 cut(s) 1565, 2939
PshBI ATTAAT 2 cut(s) 963, 1221
PsiI TTATAA 1 cut(s) 521
Psp6I CCWGG 2 cut(s) 1498, 3267
PspEI GGTNACC 1 cut(s) 935
PspFI CCCAGC 2 cut(s) 488, 2568
PspGI CCWGG 2 cut(s) 1498, 3267
PspN4I GGNNCC 4 cut(s) 913, 2952, 3057, 3224
PspPI GGNCC 2 cut(s) 2027, 2975
PspXI VCTCGAGB 1 cut(s) 2855
PstI CTGCAG 1 cut(s) 645
PstNI CAGNNNCTG 1 cut(s) 649
PsuI RGATCY 1 cut(s) 151
PsyI GACNNNGTC 3 cut(s) 799, 1781, 3209
PvuI CGATCG 1 cut(s) 2426
PvuII CAGCTG 1 cut(s) 2162
SapI GCTCTTC 1 cut(s) 741
SatI GCNGC 2 cut(s) 836, 3306
Sau96I GGNCC 2 cut(s) 2027, 2975
ScaI AGTACT 1 cut(s) 2149
SchI GAGTC 4 cut(s) 566, 640, 751, 1001
ScrFI CCNGG 4 cut(s) 1500, 1717, 1718, 3269
SduI GDGCHC 6 cut(s) 1453, 1665, 2247, 2400, 2783, 3276
SfaNI GCATC 5 cut(s) 39, 502, 1111, 1800, 2827
SfcI CTRYAG 1 cut(s) 641
Sfr274I CTCGAG 1 cut(s) 2855
SinI GGWCC 2 cut(s) 2027, 2975
SlaI CTCGAG 1 cut(s) 2855
SmaI CCCGGG 1 cut(s) 1718
SmlI CTYRAG 4 cut(s) 602, 1076, 2300, 2855
SmoI CTYRAG 4 cut(s) 602, 1076, 2300, 2855
SsiI CCGC 7 cut(s) 314, 515, 530, 1487, 1873, 2362, 3021
SspI AATATT 1 cut(s) 1277
SspMI CTAG 3 cut(s) 195, 3176, 3216
StyD4I CCNGG 4 cut(s) 1498, 1715, 1716, 3267
StyI CCWWGG 6 cut(s) 93, 270, 610, 1209, 2736, 3090
TaiI ACGT 6 cut(s) 1075, 1567, 2122, 2376, 2936, 2941
TaqII GACCGA 1 cut(s) 1399
TatI WGTACW 4 cut(s) 762, 1557, 2147, 2658
TfiI GAWTC 5 cut(s) 168, 317, 824, 3028, 3316
TscAI CASTG 9 cut(s) 772, 1333, 1348, 1406, 1458, 1866, 2254, 2788, 3208
TseFI GTSAC 2 cut(s) 935, 1571
TseI GCWGC 2 cut(s) 835, 3305
Tsp45I GTSAC 2 cut(s) 935, 1571
TspMI CCCGGG 1 cut(s) 1716
TspRI CASTG 9 cut(s) 772, 1333, 1348, 1406, 1458, 1866, 2254, 2788, 3208
Tth111I GACNNNGTC 3 cut(s) 799, 1781, 3209
Van91I CCANNNNNTGG 1 cut(s) 94
Vha464I CTTAAG 1 cut(s) 602
VneI GTGCAC 1 cut(s) 2396
VpaK11BI GGWCC 2 cut(s) 2027, 2975
VspI ATTAAT 2 cut(s) 963, 1221
XagI CCTNNNNNAGG 2 cut(s) 1826, 2225
XapI RAATTY 7 cut(s) 325, 1409, 1523, 2276, 3039, 3143, 3345
XceI RCATGY 2 cut(s) 624, 1552
XcmI CCANNNNNNNNNTGG 2 cut(s) 2303, 2743
XhoI CTCGAG 1 cut(s) 2855
XmaI CCCGGG 1 cut(s) 1716
XmiI GTMKAC 2 cut(s) 2607, 3000
XmnI GAANNNNTTC 3 cut(s) 1623, 2006, 2949
XspI CTAG 3 cut(s) 195, 3176, 3216
ZraI GACGTC 1 cut(s) 2374
ZrmI AGTACT 1 cut(s) 2149
Zsp2I ATGCAT 1 cut(s) 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.