MD05G1025700.v1.1

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000724
Physical Location & Seq
Forward (+)
4169341 .. 4169980
640 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1025700.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGGTATGACAGCGGAGGAGCTCTTCAATACTGAGCATGCAAAGCTTCTTCATTCGGCACAAACATGGATAAGAGGCACTGCTCAGTCATGCTCGGCCGTGGCAGCTCTAGTGGCCACTGTGGTCTTTGCAGCCGCCTACACTGCTCCCGGGGGTTACAGTTCAGGCGGTGCTCCTGTTCTTCGACATTCTCCCTTCTTCACTACCTTTATCATCACAGATGTTGTCTCGCTCATCAGCTCATTGTCTTCTCTCGTCACGTTTCTCTCGATCTTGACATCTCCGTTTGAGTACAAAAACTTCCATCAATCTCTTCCGCTCAGGCTGCATTTAGGATTTGCCTTTCTTTTCTTCTCGCTAGTGACGACCATGCTCGTGTTCACCGCTACAGCTGTGCTCTTGATTCATCTCGATGAGGAATTGACAAGGTCTCTCCTTTATGTTGCTGCCTTTCTTCCTGTGTCTGTATTTGGGCTCATACAGCTTCCTTTGTATGCGGGATTTTCTCATGCCCTGATATTTCTATTTAAAAAAGTTAAGAAGTTTATAATTTCGTTTTTCCCTGTAAAGGAAATCCTCAATCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

196

Amino Acids

21.42

Weight (kDa)

8.76

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 22 - 132 1.1e-19 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 548
AccBSI CCGCTC 1 cut(s) 319
AciI CCGC 6 cut(s) 14, 135, 168, 317, 384, 497
AcoI YGGCCR 2 cut(s) 96, 114
AfaI GTAC 1 cut(s) 293
AfiI CCNNNNNNNGG 1 cut(s) 568
AgsI TTSAA 1 cut(s) 28
AluBI AGCT 6 cut(s) 22, 46, 107, 240, 392, 484
AluI AGCT 6 cut(s) 22, 46, 107, 240, 392, 484
Alw21I GWGCWC 3 cut(s) 24, 175, 399
Alw26I GTCTC 2 cut(s) 232, 435
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 2 cut(s) 96, 114
ApeKI GCWGC 4 cut(s) 104, 131, 325, 446
AsuC2I CCSGG 2 cut(s) 150, 151
AsuHPI GGTGA 1 cut(s) 373
AvaI CYCGRG 1 cut(s) 149
BalI TGGCCA 1 cut(s) 116
BanII GRGCYC 2 cut(s) 24, 477
BarI GAAGNNNNNNTAC 2 cut(s) 284, 316
BauI CACGAG 1 cut(s) 374
BbsI GAAGAC 1 cut(s) 240
Bbv12I GWGCWC 3 cut(s) 24, 175, 399
BbvI GCAGC 4 cut(s) 116, 143, 312, 433
BccI CCATC 1 cut(s) 312
BceAI ACGGC 1 cut(s) 83
BcnI CCSGG 2 cut(s) 150, 151
BcoDI GTCTC 2 cut(s) 232, 435
BfaI CTAG 2 cut(s) 110, 359
BfmI CTRYAG 1 cut(s) 387
BisI GCNGC 5 cut(s) 105, 132, 135, 326, 447
BlsI GCNGC 5 cut(s) 106, 133, 136, 327, 448
Bme1390I CCNGG 2 cut(s) 150, 151
BmeT110I CYCGRG 1 cut(s) 149
BmrFI CCNGG 2 cut(s) 150, 151
BpiI GAAGAC 1 cut(s) 240
Bpu10I CCTNAGC 1 cut(s) 320
BpuMI CCSGG 2 cut(s) 150, 151
BsaI GGTCTC 1 cut(s) 435
BsaJI CCNNGG 3 cut(s) 99, 149, 150
Bsc4I CCNNNNNNNGG 1 cut(s) 568
BseDI CCNNGG 3 cut(s) 99, 149, 150
BseLI CCNNNNNNNGG 1 cut(s) 568
BseMII CTCAG 3 cut(s) 24, 98, 334
BseRI GAGGAG 1 cut(s) 32
BseX3I CGGCCG 1 cut(s) 96
BseXI GCAGC 4 cut(s) 116, 143, 312, 433
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 2 cut(s) 98, 116
BsiEI CGRYCG 1 cut(s) 99
BsiHKAI GWGCWC 3 cut(s) 24, 175, 399
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 1 cut(s) 150
BslI CCNNNNNNNGG 1 cut(s) 568
BsmAI GTCTC 2 cut(s) 232, 435
BsnI GGCC 2 cut(s) 98, 116
Bso31I GGTCTC 1 cut(s) 435
BsoBI CYCGRG 1 cut(s) 149
Bsp1286I GDGCHC 4 cut(s) 24, 175, 399, 477
Bsp143I GATC 1 cut(s) 270
BspACI CCGC 6 cut(s) 14, 135, 168, 317, 384, 497
BspANI GGCC 2 cut(s) 98, 116
BspCNI CTCAG 3 cut(s) 25, 97, 333
BspQI GCTCTTC 1 cut(s) 29
BspTNI GGTCTC 1 cut(s) 435
BsrBI CCGCTC 1 cut(s) 319
BssECI CCNNGG 3 cut(s) 99, 149, 150
BssMI GATC 1 cut(s) 270
BssSI CACGAG 1 cut(s) 374
Bst2BI CACGAG 1 cut(s) 374
Bst4CI ACNGT 2 cut(s) 121, 161
Bst6I CTCTTC 2 cut(s) 29, 318
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 3 cut(s) 33, 84, 320
BstDSI CCRYGG 1 cut(s) 99
BstKTI GATC 1 cut(s) 273
BstMAI GTCTC 2 cut(s) 232, 435
BstMBI GATC 1 cut(s) 270
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 6 cut(s) 43, 104, 113, 143, 325, 481
BstNSI RCATGY 1 cut(s) 41
BstSCI CCNGG 2 cut(s) 148, 149
BstSFI CTRYAG 1 cut(s) 387
BstV1I GCAGC 4 cut(s) 116, 143, 312, 433
BstV2I GAAGAC 1 cut(s) 240
BstZI CGGCCG 1 cut(s) 96
BsuRI GGCC 2 cut(s) 98, 116
BtgI CCRYGG 1 cut(s) 99
BtsI GCAGTG 2 cut(s) 78, 141
BtsIMutI CAGTG 3 cut(s) 78, 117, 141
Cac8I GCNNGC 1 cut(s) 39
Cfr9I CCCGGG 1 cut(s) 149
Csp6I GTAC 1 cut(s) 292
CviAII CATG 5 cut(s) 38, 66, 90, 370, 509
CviQI GTAC 1 cut(s) 292
DdeI CTNAG 3 cut(s) 33, 84, 320
DpnI GATC 1 cut(s) 272
DpnII GATC 1 cut(s) 270
DraI TTTAAA 1 cut(s) 529
EaeI YGGCCR 2 cut(s) 96, 114
EagI CGGCCG 1 cut(s) 96
Eam1104I CTCTTC 2 cut(s) 29, 318
EarI CTCTTC 2 cut(s) 29, 318
Ecl136II GAGCTC 1 cut(s) 22
EclXI CGGCCG 1 cut(s) 96
Eco24I GRGCYC 2 cut(s) 24, 477
Eco31I GGTCTC 1 cut(s) 435
Eco52I CGGCCG 1 cut(s) 96
Eco53kI GAGCTC 1 cut(s) 22
Eco88I CYCGRG 1 cut(s) 149
EcoICRI GAGCTC 1 cut(s) 22
EcoT38I GRGCYC 2 cut(s) 24, 477
FaeI CATG 5 cut(s) 41, 69, 93, 373, 512
FatI CATG 5 cut(s) 37, 65, 89, 369, 508
FauI CCCGC 1 cut(s) 490
Fnu4HI GCNGC 5 cut(s) 105, 132, 135, 326, 447
FriOI GRGCYC 2 cut(s) 24, 477
Fsp4HI GCNGC 5 cut(s) 105, 132, 135, 326, 447
FspBI CTAG 2 cut(s) 110, 359
GluI GCNGC 5 cut(s) 105, 132, 135, 326, 447
HaeIII GGCC 2 cut(s) 98, 116
HapII CCGG 1 cut(s) 150
Hin1II CATG 5 cut(s) 41, 69, 93, 373, 512
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 1 cut(s) 403
HpaII CCGG 1 cut(s) 150
HphI GGTGA 1 cut(s) 373
Hpy166II GTNNAC 1 cut(s) 381
Hpy188III TCNNGA 4 cut(s) 268, 274, 400, 410
Hpy8I GTNNAC 1 cut(s) 381
HpyAV CCTTC 1 cut(s) 205
HpyCH4III ACNGT 2 cut(s) 121, 161
HpyCH4IV ACGT 1 cut(s) 260
HpyCH4V TGCA 3 cut(s) 41, 131, 328
HpyF10VI GCNNNNNNNGC 6 cut(s) 43, 104, 113, 143, 325, 481
HpyF3I CTNAG 3 cut(s) 33, 84, 320
HpySE526I ACGT 1 cut(s) 260
Hsp92II CATG 5 cut(s) 41, 69, 93, 373, 512
Kzo9I GATC 1 cut(s) 270
LguI GCTCTTC 1 cut(s) 29
LmnI GCTCC 3 cut(s) 19, 151, 178
LpnPI CCDG 7 cut(s) 150, 163, 189, 307, 471, 527, 576
Lsp1109I GCAGC 4 cut(s) 116, 143, 312, 433
MaeI CTAG 2 cut(s) 110, 359
MaeII ACGT 1 cut(s) 260
MaeIII GTNAC 3 cut(s) 155, 256, 361
MalI GATC 1 cut(s) 272
MbiI CCGCTC 1 cut(s) 319
MboI GATC 1 cut(s) 270
MboII GAAGA 8 cut(s) 16, 41, 173, 190, 240, 305, 343, 446
MhlI GDGCHC 4 cut(s) 24, 175, 399, 477
MlsI TGGCCA 1 cut(s) 116
MluCI AATT 2 cut(s) 419, 549
MluNI TGGCCA 1 cut(s) 116
MnlI CCTC 4 cut(s) 10, 68, 409, 587
Mox20I TGGCCA 1 cut(s) 116
MscI TGGCCA 1 cut(s) 116
MseI TTAA 2 cut(s) 528, 537
MslI CAYNNNNRTG 3 cut(s) 64, 374, 411
Msp20I TGGCCA 1 cut(s) 116
MspA1I CMGCKG 2 cut(s) 14, 392
MspI CCGG 1 cut(s) 150
MspR9I CCNGG 2 cut(s) 150, 151
MwoI GCNNNNNNNGC 6 cut(s) 43, 104, 113, 143, 325, 481
NciI CCSGG 2 cut(s) 150, 151
NdeII GATC 1 cut(s) 270
NlaIII CATG 5 cut(s) 41, 69, 93, 373, 512
NmeAIII GCCGAG 1 cut(s) 74
NmuCI GTSAC 2 cut(s) 256, 361
NspI RCATGY 1 cut(s) 41
PaeI GCATGC 1 cut(s) 41
PciSI GCTCTTC 1 cut(s) 29
PcsI WCGNNNNNNNCGW 2 cut(s) 266, 362
PfeI GAWTC 1 cut(s) 403
PflFI GACNNNGTC 1 cut(s) 427
PkrI GCNGC 5 cut(s) 106, 133, 136, 327, 448
PsiI TTATAA 1 cut(s) 548
Psp124BI GAGCTC 1 cut(s) 24
PsyI GACNNNGTC 1 cut(s) 427
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
RseI CAYNNNNRTG 3 cut(s) 64, 374, 411
SacI GAGCTC 1 cut(s) 24
SapI GCTCTTC 1 cut(s) 29
SaqAI TTAA 2 cut(s) 528, 537
SatI GCNGC 5 cut(s) 105, 132, 135, 326, 447
Sau3AI GATC 1 cut(s) 270
ScrFI CCNGG 2 cut(s) 150, 151
SduI GDGCHC 4 cut(s) 24, 175, 399, 477
SetI ASST 9 cut(s) 24, 48, 109, 209, 242, 263, 394, 431, 486
SfcI CTRYAG 1 cut(s) 387
SmaI CCCGGG 1 cut(s) 151
SmiMI CAYNNNNRTG 3 cut(s) 64, 374, 411
SphI GCATGC 1 cut(s) 41
Sse9I AATT 2 cut(s) 419, 549
SsiI CCGC 6 cut(s) 14, 135, 168, 317, 384, 497
SspMI CTAG 2 cut(s) 110, 359
SstI GAGCTC 1 cut(s) 24
StyD4I CCNGG 2 cut(s) 148, 149
TaaI ACNGT 2 cut(s) 121, 161
TaiI ACGT 1 cut(s) 263
TaqI TCGA 3 cut(s) 184, 269, 411
TasI AATT 2 cut(s) 419, 549
TatI WGTACW 1 cut(s) 291
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 1 cut(s) 403
Tru1I TTAA 2 cut(s) 528, 537
Tru9I TTAA 2 cut(s) 528, 537
TscAI CASTG 3 cut(s) 85, 124, 148
TseFI GTSAC 2 cut(s) 256, 361
TseI GCWGC 4 cut(s) 104, 131, 325, 446
Tsp45I GTSAC 2 cut(s) 256, 361
TspDTI ATGAA 2 cut(s) 41, 395
TspGWI ACGGA 1 cut(s) 273
TspMI CCCGGG 1 cut(s) 149
TspRI CASTG 3 cut(s) 85, 124, 148
Tth111I GACNNNGTC 1 cut(s) 427
XceI RCATGY 1 cut(s) 41
XmaI CCCGGG 1 cut(s) 149
XspI CTAG 2 cut(s) 110, 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.