Rh5DG016800

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000724
Physical Location & Seq
Forward (+)
1299144 .. 1303725
4582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG016800.1

Sequence Viewer

Length: 2172 bp
ATGGAAGAAATACGTACTTCCGAACAATCCAAGCGCCTCTATCAAAGCCTCAGAGACGGTGATGTATTAGAAGTGGTGAAAGCTTATGCAAAAGCTACTACTGATCCTGATGCAAGATCAAAGCTAATTCCAACGGTGCACGACGACACAATCCTCCATATGGCGATTTACATGGGGCAAGTAAGCATCGCAATAGGAATCATCGAAATAAGCAGGCATAATGACAAGCTAATTACAAAACGCAATGCGTTTGGGAACACAGCTCTTCATGAGGCTGCCGCAACTGATTGTGTACTTGTTGTGGAGAAGTTGTTGGAAAAGGCACCGGCATTGCTGTCCATTCCAAACGAGAAAGGGGAAATGCCTCTCTATACAGCAGCTCAATTTGGCAAGAAAACAAATTTCATTTTGCTGGCTGAAAAACTAAAAAACAATCATCTTGATCTTCGTCCTCATTTGTACTCCCTATCTCCTTCCTACAAGTCTGTTGAAGAACTTGACGAGTTCTCCTACAGCACTGAACACATACGCAGTACCATTCTTCATGCTGCAATACATGCCGAGTTCTTTGAGCTTGCACTTTTGATAGCTAGAATGCCGGACTATGCGAAAATTGTTCAACTAAAAGACAAAAATTCGAAGACGCCTCTTCAGCTCCTCTCATCCAATTCTTCAGCATTCAATAGTGGAATGAAATGTGGACTGATCAAGTGGTTCATTTACTACTGTGCTCCATATGAGAATGCTAAAAAAGAAGATGCTCATGAACAAAATTCCAGTGATATTGATAGTCATGTTCAAAGTTCCAATAGATCAGCTACTCTATTTCTAAGGCGAGTCCTCTCTTCAGTTAATAGATCAGTTTGGATTGTTCTGCGAGAATGGCGAAAGATGGAGAAGGTTTATGACGAAAAGAAGAGGCATGGACTTGCTTTAGAATTAGCCAAGTTTCTAATTGCAAAAGACCTTTCATGGGAATCGAATCCAAAAAAAATTGCGCCATCCCAGTCAAGGACTCCAGACATACAACAATCCAATCCAAAAGCAAAACCGCCATCCCAGTCAGAGACTCCAGATATCCCATTGTTCATTGCAACAAGCAATGGAATACTAGAAATTGTGGAGGAGACACTAAGAGTCTACCCTTTGTCACTTGAGTATACAAACAAAGAGAAGCAGAACATACTGCACATCGCTATAATGCACCGTCAGTCGTACATCATTGATCGAGTGACGGGGACGAACGAGAGGTTAACGTCAAGGTTGGCTCTACAAACCGATAAGAATGGATTCACAATACTGCACTCTGTTGGAAACATGAAATTTTATAAAGGAGGCACCCAGCATGGTCCTGCTCTTCAGTTGCAAGAAGAGTTGGAATTGTATGAGAAGGTGGAGAAGTTAGTCCCACGATATTATGCAAGGAATTCTGCTGCTGAACAAAAAGAGACTCACGGAGAGTTCTATAGTCGAAAATATACGGAAACTGTAGAGGAGTTGTCTCAGAACGTGGATAAGATAATTCCACCCAAGTCTGTAAATAAGCTTGGGTGGGACGCAAGCAAAAAGGAGACCCCCAGAGAGTACTTTGGTCGAACCCATGGGGAGCTCCTCGAGGAAGCACAAGAATGGCTAAAGCAGACCTCAAACTCGTGCTCCTTGGTGGCCGCACTAATTGCCTCCGTAGCCTTCGCGGCCGCTTTCACCATCCCTGGCGGAAACAACAGCAGAAACGGCCAGCCCATCCTCATCGACAGCCCTTTCTTCTTGGTCTTCATCATCACTAACGCTCTCTCCATCGCCAGCTCGCTGATGTCACTCGCCATGTTCCTCTCCATCCTGTCGTCACCGTACGAGCTCGACGACTTCCACCGCTCTCTCCCTCGGAAGCTCATCCACGGCTTCACCTTCCTCTTCTTCTCTGTGGCCGTCACCATGCTCGCCTTTGCCTCCACAATGATGCTGACAATTCCGATAAAGAAGCGCCTGACTACAACTCTTATATACTGCGTTGCGTTTCTTCCGGTCACCATGTTTGCGCTGTTGCAGTACCCGTTGTGGGAGGCCTTCAAAAGGACTGGAAAGTCCTCGGTCAAAGTCGTGACAAGAATTCTTCCATGGCCACTTACCGAAACTGCTTGCTCAAAGGCCACTAAGGCCCACACTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

723

Amino Acids

81.69

Weight (kDa)

8.93

Isoelectric Point (pI)

46.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 543 - 655 6.4e-26 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1329
AasI GACNNNNNNGTC 1 cut(s) 2083
AbsI CCTCGAGG 1 cut(s) 1613
AccB1I GGYRCC 2 cut(s) 322, 1337
AccBSI CCGCTC 1 cut(s) 1875
AccI GTMKAC 2 cut(s) 1140, 1160
AccII CGCG 1 cut(s) 1694
AciI CCGC 7 cut(s) 279, 1052, 1668, 1694, 1698, 1716, 1873
AclWI GGATC 1 cut(s) 98
AcoI YGGCCR 5 cut(s) 1665, 1695, 1735, 1926, 2120
AcsI RAATTY 6 cut(s) 400, 634, 772, 1322, 1426, 2109
AcuI CTGAAG 4 cut(s) 635, 657, 831, 1343
AcyI GRCGYC 1 cut(s) 644
AfaI GTAC 8 cut(s) 16, 294, 461, 535, 1217, 1586, 1853, 2051
AfiI CCNNNNNNNGG 5 cut(s) 160, 973, 1011, 2059, 2073
AgsI TTSAA 5 cut(s) 491, 620, 682, 800, 2071
AjnI CCWGG 1 cut(s) 1711
Alw21I GWGCWC 5 cut(s) 141, 733, 1611, 1658, 1860
Alw26I GTCTC 6 cut(s) 48, 1061, 1121, 1442, 1506, 1565
Alw44I GTGCAC 1 cut(s) 137
AlwI GGATC 1 cut(s) 98
Ama87I CYCGRG 1 cut(s) 1613
AoxI GGCC 8 cut(s) 1665, 1695, 1735, 1926, 2064, 2120, 2148, 2157
ApaLI GTGCAC 1 cut(s) 137
ApeKI GCWGC 4 cut(s) 275, 377, 548, 1433
ApoI RAATTY 6 cut(s) 400, 634, 772, 1322, 1426, 2109
Asp700I GAANNNNTTC 1 cut(s) 1289
AspLEI GCGC 4 cut(s) 36, 1000, 1986, 2041
AspS9I GGNCC 2 cut(s) 1349, 2158
AsuHPI GGTGA 7 cut(s) 71, 88, 1696, 1839, 1897, 1924, 2020
AsuII TTCGAA 1 cut(s) 638
AvaI CYCGRG 1 cut(s) 1613
AvaII GGWCC 1 cut(s) 1349
BaeGI GKGCMC 1 cut(s) 141
BalI TGGCCA 1 cut(s) 2122
BanI GGYRCC 2 cut(s) 322, 1337
BanII GRGCYC 2 cut(s) 1611, 1860
BauI CACGAG 1 cut(s) 1651
BbsI GAAGAC 2 cut(s) 647, 1765
Bbv12I GWGCWC 5 cut(s) 141, 733, 1611, 1658, 1860
BbvI GCAGC 4 cut(s) 262, 389, 535, 1420
BccI CCATC 7 cut(s) 886, 1009, 1063, 1715, 1751, 1805, 1844
BceAI ACGGC 3 cut(s) 1750, 1913, 1915
BciT130I CCWGG 1 cut(s) 1713
BclI TGATCA 1 cut(s) 705
BcoDI GTCTC 6 cut(s) 48, 1061, 1121, 1442, 1506, 1565
BfaI CTAG 2 cut(s) 591, 1112
BfmI CTRYAG 3 cut(s) 511, 1465, 1488
BfoI RGCGCY 2 cut(s) 37, 1987
BglI GCCNNNNNGGC 2 cut(s) 1694, 2156
BisI GCNGC 8 cut(s) 276, 279, 378, 549, 1434, 1668, 1695, 1698
BlsI GCNGC 8 cut(s) 277, 280, 379, 550, 1435, 1669, 1696, 1699
BmcAI AGTACT 1 cut(s) 1586
Bme1390I CCNGG 1 cut(s) 1713
Bme18I GGWCC 1 cut(s) 1349
BmeT110I CYCGRG 1 cut(s) 1613
BmgT120I GGNCC 2 cut(s) 1349, 2158
BmiI GGNNCC 2 cut(s) 324, 1339
BmrFI CCNGG 1 cut(s) 1713
BmrI ACTGGG 2 cut(s) 1000, 1054
BmsI GCATC 4 cut(s) 100, 195, 748, 1950
BmuI ACTGGG 2 cut(s) 1000, 1054
BpiI GAAGAC 2 cut(s) 647, 1765
BpmI CTGGAG 2 cut(s) 1002, 1056
Bpu14I TTCGAA 1 cut(s) 638
BpuEI CTTGAG 1 cut(s) 1175
BsaAI YACGTR 1 cut(s) 14
BsaHI GRCGYC 1 cut(s) 644
BsaI GGTCTC 1 cut(s) 1565
BsaJI CCNNGG 7 cut(s) 1600, 1659, 1711, 1883, 1897, 2088, 2117
BsaWI WCCGGW 1 cut(s) 2023
BsaXI ACNNNNNCTCC 8 cut(s) 296, 326, 491, 521, 1640, 1670, 1778, 1808
Bsc4I CCNNNNNNNGG 5 cut(s) 160, 973, 1011, 2059, 2073
Bse118I RCCGGY 1 cut(s) 325
Bse1I ACTGG 4 cut(s) 777, 1006, 1060, 2083
Bse3DI GCAATG 4 cut(s) 250, 329, 1089, 1108
BseBI CCWGG 1 cut(s) 1713
BseDI CCNNGG 7 cut(s) 1600, 1659, 1711, 1883, 1897, 2088, 2117
BseGI GGATG 7 cut(s) 662, 1001, 1055, 1707, 1743, 1836, 1893
BseLI CCNNNNNNNGG 5 cut(s) 160, 973, 1011, 2059, 2073
BseMI GCAATG 4 cut(s) 250, 329, 1089, 1108
BseMII CTCAG 2 cut(s) 64, 1517
BseNI ACTGG 4 cut(s) 777, 1006, 1060, 2083
BseRI GAGGAG 4 cut(s) 647, 1139, 1508, 1601
BseSI GKGCMC 1 cut(s) 141
BseX3I CGGCCG 1 cut(s) 1695
BseXI GCAGC 4 cut(s) 262, 389, 535, 1420
BseYI CCCAGC 1 cut(s) 1341
BsgI GTGCAG 2 cut(s) 1172, 1286
Bsh1236I CGCG 1 cut(s) 1694
Bsh1285I CGRYCG 1 cut(s) 1698
BshFI GGCC 8 cut(s) 1667, 1697, 1737, 1928, 2066, 2122, 2150, 2159
BshNI GGYRCC 2 cut(s) 322, 1337
BsiEI CGRYCG 1 cut(s) 1698
BsiHKAI GWGCWC 5 cut(s) 141, 733, 1611, 1658, 1860
BsiHKCI CYCGRG 1 cut(s) 1613
BsiSI CCGG 3 cut(s) 326, 599, 2024
BsiWI CGTACG 1 cut(s) 1851
BslFI GGGAC 3 cut(s) 1252, 1391, 1568
BslI CCNNNNNNNGG 5 cut(s) 160, 973, 1011, 2059, 2073
BsmAI GTCTC 6 cut(s) 48, 1061, 1121, 1442, 1506, 1565
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 3 cut(s) 1252, 1391, 1568
BsmI GAATGC 3 cut(s) 600, 677, 748
BsnI GGCC 8 cut(s) 1667, 1697, 1737, 1928, 2066, 2122, 2150, 2159
Bso31I GGTCTC 1 cut(s) 1565
BsoBI CYCGRG 1 cut(s) 1613
Bsp119I TTCGAA 1 cut(s) 638
Bsp1286I GDGCHC 5 cut(s) 141, 733, 1611, 1658, 1860
Bsp143I GATC 7 cut(s) 103, 116, 442, 705, 812, 857, 1225
Bsp19I CCATGG 2 cut(s) 1600, 2117
BspACI CCGC 7 cut(s) 279, 1052, 1668, 1694, 1698, 1716, 1873
BspANI GGCC 8 cut(s) 1667, 1697, 1737, 1928, 2066, 2122, 2150, 2159
BspCNI CTCAG 2 cut(s) 63, 1516
BspFNI CGCG 1 cut(s) 1694
BspHI TCATGA 2 cut(s) 268, 763
BspLI GGNNCC 2 cut(s) 324, 1339
BspPI GGATC 1 cut(s) 98
BspQI GCTCTTC 2 cut(s) 270, 1362
BspT104I TTCGAA 1 cut(s) 638
BspT107I GGYRCC 2 cut(s) 322, 1337
BspTNI GGTCTC 1 cut(s) 1565
BsrBI CCGCTC 1 cut(s) 1875
BsrDI GCAATG 4 cut(s) 250, 329, 1089, 1108
BsrFI RCCGGY 1 cut(s) 325
BsrI ACTGG 4 cut(s) 777, 1006, 1060, 2083
BssAI RCCGGY 1 cut(s) 325
BssECI CCNNGG 7 cut(s) 1600, 1659, 1711, 1883, 1897, 2088, 2117
BssMI GATC 7 cut(s) 103, 116, 442, 705, 812, 857, 1225
BssNAI GTATAC 1 cut(s) 1161
BssNI GRCGYC 1 cut(s) 644
BssSI CACGAG 1 cut(s) 1651
BssT1I CCWWGG 3 cut(s) 1600, 1659, 2117
Bst1107I GTATAC 1 cut(s) 1161
Bst2BI CACGAG 1 cut(s) 1651
Bst2UI CCWGG 1 cut(s) 1713
Bst4CI ACNGT 6 cut(s) 59, 136, 728, 1208, 1489, 1851
Bst6I CTCTTC 7 cut(s) 270, 654, 850, 911, 1362, 1365, 1919
BstACI GRCGYC 1 cut(s) 644
BstAPI GCANNNNNTGC 2 cut(s) 557, 1676
BstBAI YACGTR 1 cut(s) 14
BstBI TTCGAA 1 cut(s) 638
BstC8I GCNNGC 9 cut(s) 215, 414, 576, 1561, 1739, 1804, 1808, 1941, 2140
BstDEI CTNAG 5 cut(s) 50, 830, 1133, 1503, 2154
BstDSI CCRYGG 3 cut(s) 1600, 1897, 2117
BstEII GGTNACC 1 cut(s) 2026
BstENI CCTNNNNNAGG 1 cut(s) 2071
BstF5I GGATG 7 cut(s) 662, 1001, 1055, 1707, 1743, 1836, 1893
BstFNI CGCG 1 cut(s) 1694
BstH2I RGCGCY 2 cut(s) 37, 1987
BstHHI GCGC 4 cut(s) 36, 1000, 1986, 2041
BstKTI GATC 7 cut(s) 106, 119, 445, 708, 815, 860, 1228
BstMAI GTCTC 6 cut(s) 48, 1061, 1121, 1442, 1506, 1565
BstMBI GATC 7 cut(s) 103, 116, 442, 705, 812, 857, 1225
BstMCI CGRYCG 1 cut(s) 1698
BstMWI GCNNNNNNNGC 8 cut(s) 557, 652, 883, 1676, 1685, 1694, 1734, 2156
BstNI CCWGG 1 cut(s) 1713
BstNSI RCATGY 1 cut(s) 560
BstPI GGTNACC 1 cut(s) 2026
BstSCI CCNGG 1 cut(s) 1711
BstSFI CTRYAG 3 cut(s) 511, 1465, 1488
BstSLI GKGCMC 1 cut(s) 141
BstSNI TACGTA 1 cut(s) 14
BstUI CGCG 1 cut(s) 1694
BstV1I GCAGC 4 cut(s) 262, 389, 535, 1420
BstV2I GAAGAC 2 cut(s) 647, 1765
BstZ17I GTATAC 1 cut(s) 1161
BstZI CGGCCG 1 cut(s) 1695
BsuRI GGCC 8 cut(s) 1667, 1697, 1737, 1928, 2066, 2122, 2150, 2159
BtgI CCRYGG 3 cut(s) 1600, 1897, 2117
BtgZI GCGATG 3 cut(s) 172, 1177, 1783
BtsCI GGATG 7 cut(s) 662, 1001, 1055, 1707, 1743, 1836, 1893
BtsIMutI CAGTG 2 cut(s) 516, 784
Cac8I GCNNGC 9 cut(s) 215, 414, 576, 1561, 1739, 1804, 1808, 1941, 2140
CciI TCATGA 2 cut(s) 268, 763
CciNI GCGGCCGC 1 cut(s) 1695
CfoI GCGC 4 cut(s) 36, 1000, 1986, 2041
Cfr10I RCCGGY 1 cut(s) 325
Cfr13I GGNCC 2 cut(s) 1349, 2158
CseI GACGC 2 cut(s) 652, 1565
Csp6I GTAC 8 cut(s) 15, 293, 460, 534, 1216, 1585, 1852, 2050
CviQI GTAC 8 cut(s) 15, 293, 460, 534, 1216, 1585, 1852, 2050
DdeI CTNAG 5 cut(s) 50, 830, 1133, 1503, 2154
DpnI GATC 7 cut(s) 105, 118, 444, 707, 814, 859, 1227
DpnII GATC 7 cut(s) 103, 116, 442, 705, 812, 857, 1225
DrdI GACNNNNNNGTC 1 cut(s) 2083
DseDI GACNNNNNNGTC 1 cut(s) 2083
EaeI YGGCCR 5 cut(s) 1665, 1695, 1735, 1926, 2120
EagI CGGCCG 1 cut(s) 1695
Eam1104I CTCTTC 7 cut(s) 270, 654, 850, 911, 1362, 1365, 1919
EarI CTCTTC 7 cut(s) 270, 654, 850, 911, 1362, 1365, 1919
EciI GGCGGA 1 cut(s) 1731
Ecl136II GAGCTC 2 cut(s) 1609, 1858
EclXI CGGCCG 1 cut(s) 1695
Eco105I TACGTA 1 cut(s) 14
Eco130I CCWWGG 3 cut(s) 1600, 1659, 2117
Eco147I AGGCCT 1 cut(s) 2066
Eco24I GRGCYC 2 cut(s) 1611, 1860
Eco31I GGTCTC 1 cut(s) 1565
Eco32I GATATC 1 cut(s) 1078
Eco47I GGWCC 1 cut(s) 1349
Eco52I CGGCCG 1 cut(s) 1695
Eco53kI GAGCTC 2 cut(s) 1609, 1858
Eco57I CTGAAG 4 cut(s) 635, 657, 831, 1343
Eco88I CYCGRG 1 cut(s) 1613
Eco91I GGTNACC 1 cut(s) 2026
EcoICRI GAGCTC 2 cut(s) 1609, 1858
EcoNI CCTNNNNNAGG 1 cut(s) 2071
EcoO65I GGTNACC 1 cut(s) 2026
EcoRI GAATTC 2 cut(s) 1426, 2109
EcoRII CCWGG 1 cut(s) 1711
EcoRV GATATC 1 cut(s) 1078
EcoT14I CCWWGG 3 cut(s) 1600, 1659, 2117
EcoT38I GRGCYC 2 cut(s) 1611, 1860
ErhI CCWWGG 3 cut(s) 1600, 1659, 2117
Esp3I CGTCTC 1 cut(s) 48
FaqI GGGAC 3 cut(s) 1252, 1391, 1568
FauNDI CATATG 2 cut(s) 159, 736
FbaI TGATCA 1 cut(s) 705
FblI GTMKAC 2 cut(s) 1140, 1160
Fnu4HI GCNGC 8 cut(s) 276, 279, 378, 549, 1434, 1668, 1695, 1698
FokI GGATG 7 cut(s) 649, 988, 1042, 1694, 1730, 1823, 1880
FriOI GRGCYC 2 cut(s) 1611, 1860
Fsp4HI GCNGC 8 cut(s) 276, 279, 378, 549, 1434, 1668, 1695, 1698
FspBI CTAG 2 cut(s) 591, 1112
GlaI GCGC 4 cut(s) 35, 999, 1985, 2040
GluI GCNGC 8 cut(s) 276, 279, 378, 549, 1434, 1668, 1695, 1698
GsaI CCCAGC 1 cut(s) 1345
GsuI CTGGAG 2 cut(s) 1002, 1056
HaeII RGCGCY 2 cut(s) 37, 1987
HaeIII GGCC 8 cut(s) 1667, 1697, 1737, 1928, 2066, 2122, 2150, 2159
HapII CCGG 3 cut(s) 326, 599, 2024
HgaI GACGC 2 cut(s) 652, 1565
HhaI GCGC 4 cut(s) 36, 1000, 1986, 2041
Hin1I GRCGYC 1 cut(s) 644
Hin6I GCGC 4 cut(s) 34, 998, 1984, 2039
HinP1I GCGC 4 cut(s) 34, 998, 1984, 2039
HincII GTYRAC 1 cut(s) 1254
HindII GTYRAC 1 cut(s) 1254
HindIII AAGCTT 2 cut(s) 81, 1544
HinfI GANTC 9 cut(s) 198, 837, 977, 982, 1015, 1069, 1137, 1290, 1450
HpaI GTTAAC 1 cut(s) 1254
HpaII CCGG 3 cut(s) 326, 599, 2024
HphI GGTGA 7 cut(s) 71, 88, 1696, 1839, 1897, 1924, 2020
Hpy166II GTNNAC 6 cut(s) 139, 293, 701, 1141, 1161, 1254
Hpy188I TCNGA 6 cut(s) 22, 53, 1066, 1506, 1887, 1974
Hpy188III TCNNGA 7 cut(s) 107, 269, 440, 764, 1019, 1073, 2101
Hpy8I GTNNAC 6 cut(s) 139, 293, 701, 1141, 1161, 1254
Hpy99I CGWCG 2 cut(s) 146, 1865
HpyAV CCTTC 6 cut(s) 483, 892, 1384, 1699, 1918, 2077
HpyCH4III ACNGT 6 cut(s) 59, 136, 728, 1208, 1489, 1851
HpyCH4IV ACGT 3 cut(s) 13, 1256, 1509
HpyF10VI GCNNNNNNNGC 8 cut(s) 557, 652, 883, 1676, 1685, 1694, 1734, 2156
HpyF3I CTNAG 5 cut(s) 50, 830, 1133, 1503, 2154
HpySE526I ACGT 3 cut(s) 13, 1256, 1509
Hsp92I GRCGYC 1 cut(s) 644
HspAI GCGC 4 cut(s) 34, 998, 1984, 2039
Ksp22I TGATCA 1 cut(s) 705
KspAI GTTAAC 1 cut(s) 1254
Kzo9I GATC 7 cut(s) 103, 116, 442, 705, 812, 857, 1225
LguI GCTCTTC 2 cut(s) 270, 1362
LmnI GCTCC 5 cut(s) 660, 736, 1606, 1614, 1661
Lsp1109I GCAGC 4 cut(s) 262, 389, 535, 1420
LweI GCATC 4 cut(s) 100, 195, 748, 1950
MaeI CTAG 2 cut(s) 591, 1112
MaeII ACGT 3 cut(s) 13, 1256, 1509
MaeIII GTNAC 7 cut(s) 1149, 1231, 1815, 1845, 1930, 2026, 2101
MalI GATC 7 cut(s) 105, 118, 444, 707, 814, 859, 1227
MbiI CCGCTC 1 cut(s) 1875
MboI GATC 7 cut(s) 103, 116, 442, 705, 812, 857, 1225
MhlI GDGCHC 5 cut(s) 141, 733, 1611, 1658, 1860
MlsI TGGCCA 1 cut(s) 2122
MluNI TGGCCA 1 cut(s) 2122
MlyI GAGTC 5 cut(s) 846, 1009, 1063, 1146, 1444
MmeI TCCRAC 4 cut(s) 155, 294, 1291, 1356
Mox20I TGGCCA 1 cut(s) 2122
MroXI GAANNNNTTC 1 cut(s) 1289
MscI TGGCCA 1 cut(s) 2122
MseI TTAA 2 cut(s) 852, 1253
MslI CAYNNNNRTG 2 cut(s) 1627, 1958
Msp20I TGGCCA 1 cut(s) 2122
MspI CCGG 3 cut(s) 326, 599, 2024
MspR9I CCNGG 1 cut(s) 1713
Mva1269I GAATGC 3 cut(s) 600, 677, 748
MvaI CCWGG 1 cut(s) 1713
MvnI CGCG 1 cut(s) 1694
MwoI GCNNNNNNNGC 8 cut(s) 557, 652, 883, 1676, 1685, 1694, 1734, 2156
NcoI CCATGG 2 cut(s) 1600, 2117
NdeI CATATG 2 cut(s) 159, 736
NdeII GATC 7 cut(s) 103, 116, 442, 705, 812, 857, 1225
NlaIV GGNNCC 2 cut(s) 324, 1339
NmeAIII GCCGAG 1 cut(s) 586
NmuCI GTSAC 7 cut(s) 1149, 1231, 1815, 1845, 1930, 2026, 2101
NotI GCGGCCGC 1 cut(s) 1695
NspI RCATGY 1 cut(s) 560
NspV TTCGAA 1 cut(s) 638
PaeR7I CTCGAG 1 cut(s) 1613
PagI TCATGA 2 cut(s) 268, 763
PceI AGGCCT 1 cut(s) 2066
PciSI GCTCTTC 2 cut(s) 270, 1362
PcsI WCGNNNNNNNCGW 1 cut(s) 1860
PctI GAATGC 3 cut(s) 600, 677, 748
PdmI GAANNNNTTC 1 cut(s) 1289
PfeI GAWTC 4 cut(s) 198, 977, 982, 1290
Pfl23II CGTACG 1 cut(s) 1851
PkrI GCNGC 8 cut(s) 277, 280, 379, 550, 1435, 1669, 1696, 1699
PleI GAGTC 5 cut(s) 845, 1009, 1063, 1145, 1444
PpsI GAGTC 5 cut(s) 845, 1009, 1063, 1145, 1444
Ppu21I YACGTR 1 cut(s) 14
PsiI TTATAA 1 cut(s) 1329
Psp124BI GAGCTC 2 cut(s) 1611, 1860
Psp6I CCWGG 1 cut(s) 1711
PspEI GGTNACC 1 cut(s) 2026
PspFI CCCAGC 1 cut(s) 1341
PspGI CCWGG 1 cut(s) 1711
PspLI CGTACG 1 cut(s) 1851
PspN4I GGNNCC 2 cut(s) 324, 1339
PspPI GGNCC 2 cut(s) 1349, 2158
PspXI VCTCGAGB 1 cut(s) 1613
RsaI GTAC 8 cut(s) 16, 294, 461, 535, 1217, 1586, 1853, 2051
RsaNI GTAC 8 cut(s) 15, 293, 460, 534, 1216, 1585, 1852, 2050
RseI CAYNNNNRTG 2 cut(s) 1627, 1958
SacI GAGCTC 2 cut(s) 1611, 1860
SapI GCTCTTC 2 cut(s) 270, 1362
SaqAI TTAA 2 cut(s) 852, 1253
SatI GCNGC 8 cut(s) 276, 279, 378, 549, 1434, 1668, 1695, 1698
Sau3AI GATC 7 cut(s) 103, 116, 442, 705, 812, 857, 1225
Sau96I GGNCC 2 cut(s) 1349, 2158
ScaI AGTACT 1 cut(s) 1586
SchI GAGTC 5 cut(s) 846, 1009, 1063, 1146, 1444
ScrFI CCNGG 1 cut(s) 1713
SduI GDGCHC 5 cut(s) 141, 733, 1611, 1658, 1860
SfaNI GCATC 4 cut(s) 100, 195, 748, 1950
SfcI CTRYAG 3 cut(s) 511, 1465, 1488
SfiI GGCCNNNNNGGCC 1 cut(s) 2156
Sfr274I CTCGAG 1 cut(s) 1613
SfuI TTCGAA 1 cut(s) 638
SinI GGWCC 1 cut(s) 1349
SlaI CTCGAG 1 cut(s) 1613
SmiMI CAYNNNNRTG 2 cut(s) 1627, 1958
SmlI CTYRAG 2 cut(s) 1154, 1613
SmoI CTYRAG 2 cut(s) 1154, 1613
SnaBI TACGTA 1 cut(s) 14
SseBI AGGCCT 1 cut(s) 2066
SsiI CCGC 7 cut(s) 279, 1052, 1668, 1694, 1698, 1716, 1873
SspMI CTAG 2 cut(s) 591, 1112
SstI GAGCTC 2 cut(s) 1611, 1860
StuI AGGCCT 1 cut(s) 2066
StyD4I CCNGG 1 cut(s) 1711
StyI CCWWGG 3 cut(s) 1600, 1659, 2117
TaaI ACNGT 6 cut(s) 59, 136, 728, 1208, 1489, 1851
TaiI ACGT 3 cut(s) 16, 1259, 1512
TaqI TCGA 9 cut(s) 204, 638, 980, 1228, 1471, 1594, 1614, 1752, 1860
TaqII GACCGA 1 cut(s) 2080
TatI WGTACW 3 cut(s) 292, 459, 1584
TauI GCSGC 4 cut(s) 281, 1670, 1697, 1700
TfiI GAWTC 4 cut(s) 198, 977, 982, 1290
Tru1I TTAA 2 cut(s) 852, 1253
Tru9I TTAA 2 cut(s) 852, 1253
TscAI CASTG 2 cut(s) 523, 784
TseFI GTSAC 7 cut(s) 1149, 1231, 1815, 1845, 1930, 2026, 2101
TseI GCWGC 4 cut(s) 275, 377, 548, 1433
Tsp45I GTSAC 7 cut(s) 1149, 1231, 1815, 1845, 1930, 2026, 2101
TspGWI ACGGA 3 cut(s) 1470, 1496, 1672
TspRI CASTG 2 cut(s) 523, 784
VneI GTGCAC 1 cut(s) 137
VpaK11BI GGWCC 1 cut(s) 1349
XagI CCTNNNNNAGG 1 cut(s) 2071
XapI RAATTY 6 cut(s) 400, 634, 772, 1322, 1426, 2109
XceI RCATGY 1 cut(s) 560
XhoI CTCGAG 1 cut(s) 1613
XmiI GTMKAC 2 cut(s) 1140, 1160
XmnI GAANNNNTTC 1 cut(s) 1289
XspI CTAG 2 cut(s) 591, 1112
ZrmI AGTACT 1 cut(s) 1586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.