MD10G1327700.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000724
Physical Location & Seq
Reverse (-)
40724980 .. 40725864
885 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1327700.v1.1.491

Sequence Viewer

Length: 693 bp
ATGCAATTGCAAGAAGAGCTACGTTGGATGCTGTGTGTAAAAGAGATACTACCGCCTCGGTACACCTTGCACCGCAACAAGAAGGATCAGACAACTGAGGAGCTGTTCAACTACCAGCATAACGGTCTTCTGAGGTCGGCACAAGAATGGGTAAAAGAAACGGCTCAGTCATGCTCAACTGTGGCGGTGCTAGTGTCCACTGTGGTCTTTGCAGCTGCCTACGCCATTCCCGGGGGTACTAGTGACCAAAGTGGCCTTCCTCTTTTCCGAGACGATCCTCTCTTTTTGTTCTTCACCTGCATGGACGTTGTGGGCATTGCCTGCTCATTATCTTCTGTGGCATTCTTTCTCTCTGTCCTCTCCTCCCCTCTTGAGTACCCATATTTCGTTAACAGTGTTCCTCGCAAGATCATGACTGGATTCATCTTGCTTTTCTTGTCAATGGCATCCACCATGCTCGCCTTTGCCGCCACCATTTTGCTCTTGATTCGTGTAGAGAAGAAATGGACCAAGTCTGTACTTTACCCTATTGCATTTTTCCCAGTCCCTCTATTCGGCCTGCTGCAATTTCCTATGTATCAATCTTTCATAGTCATGTTTCGGAAAATTGATGACTGGATTTTCAGCCCCTTACGCTGCCCTCTCGGCTTGTCCAAGAGGAGATCAATATGGGGCAAGCGTAAGGCGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

231

Amino Acids

26.32

Weight (kDa)

9.34

Isoelectric Point (pI)

58.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 49 - 160 2.5e-20 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 305
Acc36I ACCTGC 1 cut(s) 305
AciI CCGC 4 cut(s) 53, 73, 185, 468
AclWI GGATC 2 cut(s) 93, 269
AfaI GTAC 4 cut(s) 62, 238, 377, 519
AfiI CCNNNNNNNGG 2 cut(s) 231, 554
AgsI TTSAA 1 cut(s) 109
AhlI ACTAGT 1 cut(s) 239
AjuI GAANNNNNNNTTGG 2 cut(s) 240, 272
AluBI AGCT 3 cut(s) 19, 103, 215
AluI AGCT 3 cut(s) 19, 103, 215
Alw26I GTCTC 1 cut(s) 264
AlwI GGATC 2 cut(s) 93, 269
Ama87I CYCGRG 1 cut(s) 230
AoxI GGCC 2 cut(s) 253, 556
ApeKI GCWGC 4 cut(s) 212, 215, 562, 636
AspS9I GGNCC 1 cut(s) 507
AsuC2I CCSGG 2 cut(s) 231, 232
AsuHPI GGTGA 1 cut(s) 286
AvaI CYCGRG 1 cut(s) 230
AvaII GGWCC 1 cut(s) 507
BbsI GAAGAC 1 cut(s) 119
BbvI GCAGC 4 cut(s) 202, 224, 549, 623
BceAI ACGGC 1 cut(s) 177
BcnI CCSGG 2 cut(s) 231, 232
BcoDI GTCTC 1 cut(s) 264
BcuI ACTAGT 1 cut(s) 239
BfaI CTAG 2 cut(s) 191, 240
BfuAI ACCTGC 1 cut(s) 305
BglI GCCNNNNNGGC 1 cut(s) 645
BisI GCNGC 5 cut(s) 213, 216, 468, 563, 637
BlsI GCNGC 5 cut(s) 214, 217, 469, 564, 638
Bme1390I CCNGG 2 cut(s) 231, 232
Bme18I GGWCC 1 cut(s) 507
BmeT110I CYCGRG 1 cut(s) 230
BmgT120I GGNCC 1 cut(s) 507
BmrFI CCNGG 2 cut(s) 231, 232
BmrI ACTGGG 1 cut(s) 536
BmsI GCATC 2 cut(s) 18, 455
BmuI ACTGGG 1 cut(s) 536
BpiI GAAGAC 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 392
BpuMI CCSGG 2 cut(s) 231, 232
BsaJI CCNNGG 3 cut(s) 56, 230, 231
Bsc4I CCNNNNNNNGG 2 cut(s) 231, 554
Bse1I ACTGG 3 cut(s) 421, 542, 620
Bse3DI GCAATG 1 cut(s) 315
BseDI CCNNGG 3 cut(s) 56, 230, 231
BseGI GGATG 2 cut(s) 33, 446
BseLI CCNNNNNNNGG 2 cut(s) 231, 554
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 3 cut(s) 87, 122, 179
BseNI ACTGG 3 cut(s) 421, 542, 620
BseRI GAGGAG 3 cut(s) 113, 352, 673
BseXI GCAGC 4 cut(s) 202, 224, 549, 623
BshFI GGCC 2 cut(s) 255, 558
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 1 cut(s) 231
BslFI GGGAC 1 cut(s) 530
BslI CCNNNNNNNGG 2 cut(s) 231, 554
BsmAI GTCTC 1 cut(s) 264
BsmBI CGTCTC 1 cut(s) 264
BsmFI GGGAC 1 cut(s) 530
BsmI GAATGC 1 cut(s) 341
BsnI GGCC 2 cut(s) 255, 558
BsoBI CYCGRG 1 cut(s) 230
Bsp143I GATC 4 cut(s) 85, 274, 408, 662
BspACI CCGC 4 cut(s) 53, 73, 185, 468
BspANI GGCC 2 cut(s) 255, 558
BspCNI CTCAG 3 cut(s) 88, 123, 178
BspHI TCATGA 1 cut(s) 411
BspMI ACCTGC 1 cut(s) 305
BspPI GGATC 2 cut(s) 93, 269
BspQI GCTCTTC 1 cut(s) 9
BsrDI GCAATG 1 cut(s) 315
BsrI ACTGG 3 cut(s) 421, 542, 620
BssECI CCNNGG 3 cut(s) 56, 230, 231
BssMI GATC 4 cut(s) 85, 274, 408, 662
Bst4CI ACNGT 4 cut(s) 125, 181, 202, 395
Bst6I CTCTTC 1 cut(s) 9
BstAPI GCANNNNNTGC 1 cut(s) 321
BstC8I GCNNGC 4 cut(s) 322, 459, 560, 677
BstDEI CTNAG 3 cut(s) 96, 131, 165
BstF5I GGATG 2 cut(s) 33, 446
BstKTI GATC 4 cut(s) 88, 277, 411, 665
BstMAI GTCTC 1 cut(s) 264
BstMBI GATC 4 cut(s) 85, 274, 408, 662
BstMWI GCNNNNNNNGC 6 cut(s) 16, 221, 321, 467, 633, 645
BstSCI CCNGG 2 cut(s) 229, 230
BstV1I GCAGC 4 cut(s) 202, 224, 549, 623
BstV2I GAAGAC 1 cut(s) 119
BsuRI GGCC 2 cut(s) 255, 558
BtsCI GGATG 2 cut(s) 33, 446
BtsIMutI CAGTG 2 cut(s) 198, 400
BveI ACCTGC 1 cut(s) 305
Cac8I GCNNGC 4 cut(s) 322, 459, 560, 677
CciI TCATGA 1 cut(s) 411
Cfr13I GGNCC 1 cut(s) 507
Cfr9I CCCGGG 1 cut(s) 230
Csp6I GTAC 4 cut(s) 61, 237, 376, 518
CviAII CATG 5 cut(s) 171, 301, 412, 454, 595
CviJI RGCY 8 cut(s) 19, 103, 164, 215, 255, 558, 627, 648
CviKI_1 RGCY 8 cut(s) 19, 103, 164, 215, 255, 558, 627, 648
CviQI GTAC 4 cut(s) 61, 237, 376, 518
DdeI CTNAG 3 cut(s) 96, 131, 165
DpnI GATC 4 cut(s) 87, 276, 410, 664
DpnII GATC 4 cut(s) 85, 274, 408, 662
Eam1104I CTCTTC 1 cut(s) 9
EarI CTCTTC 1 cut(s) 9
Eco47I GGWCC 1 cut(s) 507
Eco88I CYCGRG 1 cut(s) 230
Esp3I CGTCTC 1 cut(s) 264
FaeI CATG 5 cut(s) 174, 304, 415, 457, 598
FaqI GGGAC 1 cut(s) 530
FatI CATG 5 cut(s) 170, 300, 411, 453, 594
Fnu4HI GCNGC 5 cut(s) 213, 216, 468, 563, 637
FokI GGATG 2 cut(s) 40, 433
Fsp4HI GCNGC 5 cut(s) 213, 216, 468, 563, 637
FspBI CTAG 2 cut(s) 191, 240
GluI GCNGC 5 cut(s) 213, 216, 468, 563, 637
HaeIII GGCC 2 cut(s) 255, 558
HapII CCGG 1 cut(s) 231
Hin1II CATG 5 cut(s) 174, 304, 415, 457, 598
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HinfI GANTC 2 cut(s) 420, 487
HpaI GTTAAC 1 cut(s) 391
HpaII CCGG 1 cut(s) 231
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 3 cut(s) 63, 198, 391
Hpy188I TCNGA 4 cut(s) 90, 132, 269, 603
Hpy188III TCNNGA 3 cut(s) 371, 412, 484
Hpy8I GTNNAC 3 cut(s) 63, 198, 391
HpyAV CCTTC 2 cut(s) 76, 266
HpyCH4III ACNGT 4 cut(s) 125, 181, 202, 395
HpyCH4IV ACGT 2 cut(s) 22, 306
HpyCH4V TGCA 7 cut(s) 4, 10, 70, 212, 300, 533, 565
HpyF10VI GCNNNNNNNGC 6 cut(s) 16, 221, 321, 467, 633, 645
HpyF3I CTNAG 3 cut(s) 96, 131, 165
HpySE526I ACGT 2 cut(s) 22, 306
Hsp92II CATG 5 cut(s) 174, 304, 415, 457, 598
KspAI GTTAAC 1 cut(s) 391
Kzo9I GATC 4 cut(s) 85, 274, 408, 662
LguI GCTCTTC 1 cut(s) 9
LmnI GCTCC 1 cut(s) 100
LpnPI CCDG 8 cut(s) 128, 244, 310, 334, 402, 555, 572, 601
Lsp1109I GCAGC 4 cut(s) 202, 224, 549, 623
LweI GCATC 2 cut(s) 18, 455
MaeI CTAG 2 cut(s) 191, 240
MaeII ACGT 2 cut(s) 22, 306
MaeIII GTNAC 1 cut(s) 242
MalI GATC 4 cut(s) 87, 276, 410, 664
MboI GATC 4 cut(s) 85, 274, 408, 662
MboII GAAGA 5 cut(s) 26, 119, 283, 324, 511
MfeI CAATTG 1 cut(s) 5
MluCI AATT 3 cut(s) 5, 566, 606
MmeI TCCRAC 1 cut(s) 5
MseI TTAA 1 cut(s) 390
MslI CAYNNNNRTG 3 cut(s) 145, 299, 593
MspA1I CMGCKG 1 cut(s) 215
MspI CCGG 1 cut(s) 231
MspR9I CCNGG 2 cut(s) 231, 232
MunI CAATTG 1 cut(s) 5
Mva1269I GAATGC 1 cut(s) 341
MwoI GCNNNNNNNGC 6 cut(s) 16, 221, 321, 467, 633, 645
NciI CCSGG 2 cut(s) 231, 232
NdeII GATC 4 cut(s) 85, 274, 408, 662
NlaIII CATG 5 cut(s) 174, 304, 415, 457, 598
NmeAIII GCCGAG 1 cut(s) 624
NmuCI GTSAC 1 cut(s) 242
PagI TCATGA 1 cut(s) 411
PaqCI CACCTGC 1 cut(s) 305
PciSI GCTCTTC 1 cut(s) 9
PctI GAATGC 1 cut(s) 341
PfeI GAWTC 2 cut(s) 420, 487
PflFI GACNNNGTC 1 cut(s) 511
PkrI GCNGC 5 cut(s) 214, 217, 469, 564, 638
PspPI GGNCC 1 cut(s) 507
PsyI GACNNNGTC 1 cut(s) 511
PvuII CAGCTG 1 cut(s) 215
RsaI GTAC 4 cut(s) 62, 238, 377, 519
RsaNI GTAC 4 cut(s) 61, 237, 376, 518
RseI CAYNNNNRTG 3 cut(s) 145, 299, 593
SapI GCTCTTC 1 cut(s) 9
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 5 cut(s) 213, 216, 468, 563, 637
Sau3AI GATC 4 cut(s) 85, 274, 408, 662
Sau96I GGNCC 1 cut(s) 507
ScrFI CCNGG 2 cut(s) 231, 232
SetI ASST 8 cut(s) 21, 25, 68, 105, 137, 217, 299, 309
SfaNI GCATC 2 cut(s) 18, 455
SinI GGWCC 1 cut(s) 507
SmaI CCCGGG 1 cut(s) 232
SmiMI CAYNNNNRTG 3 cut(s) 145, 299, 593
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
SpeI ACTAGT 1 cut(s) 239
Sse9I AATT 3 cut(s) 5, 566, 606
SsiI CCGC 4 cut(s) 53, 73, 185, 468
SspMI CTAG 2 cut(s) 191, 240
StyD4I CCNGG 2 cut(s) 229, 230
TaaI ACNGT 4 cut(s) 125, 181, 202, 395
TaiI ACGT 2 cut(s) 25, 309
TasI AATT 3 cut(s) 5, 566, 606
TatI WGTACW 1 cut(s) 517
TauI GCSGC 1 cut(s) 470
TfiI GAWTC 2 cut(s) 420, 487
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TscAI CASTG 2 cut(s) 205, 400
TseFI GTSAC 1 cut(s) 242
TseI GCWGC 4 cut(s) 212, 215, 562, 636
Tsp45I GTSAC 1 cut(s) 242
TspDTI ATGAA 2 cut(s) 412, 577
TspMI CCCGGG 1 cut(s) 230
TspRI CASTG 2 cut(s) 205, 400
Tth111I GACNNNGTC 1 cut(s) 511
VpaK11BI GGWCC 1 cut(s) 507
XmaI CCCGGG 1 cut(s) 230
XspI CTAG 2 cut(s) 191, 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.