Rh5BG018500

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000724
Physical Location & Seq
Forward (+)
1364536 .. 1368938
4403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG018500.1

Sequence Viewer

Length: 2154 bp
ATGGAAGAAATACGTACTTCCGAACAATCCAAAGCCCTCTATCAAAGCCTCAGAGACGGTGACGTATTAGAAGTGGTGAAAGCTTATGCAAAAGCTACTACTCTTGATGCAACATCGATATCAAAGCTAATTCCAACAGTGCACGACGACACAATCCTCCATATGGCGATTTACATGAAGCAAGAAAGCATCGCAATAGGAATCATCGAAATAAGCAGGCATAATGACGAGCTAATTAAAAAACCCAATGCGTTTGGAAACACAGTTCTTCATGAGGCTGCAGCAACTGATTGTGTACGTGTTGTGGAGAAGTTGTTGGAAAAGGCACCGGATTTGCTGTCCATTCCAAACAATGAAGGGGAAATGCCTCTCTATACAGCAGCTCACTTTGGCCAGAAAGAAATTTTCATTTTGCTGGCTGAAAAACTAAAAAACAATGATTATGATCTAATTCGTCCCCATTTGTGCTCCCTAAAAGGTTCCTCCATATGCAGTACCGTTCTTCATGCTGCAATACATGCCGAGTTCTTTGAGCTTGCACTTTGGATAGCTAGAAAGCCGGAGTATGCGAAGATTGTTCAACTAAAAGACAAAAATTTGAAGACGCCTCTTCAGCTCCTCTCATCCAATTCTTCAGCATTCAAGAGTGGAATGAAATGTGGACTGATCAAGAGGTTCATTTACAACTGTGCTCCATATGAAGATGCTCATGAACAAAATTCCAGTGATGATAGTCATGTTCAAAGTTTCAAAAGATCAGCTGCTCTGTTTCTAAACTTTCTACGGCGAGTCTTCTCTTCAGTTAATAGATCGGTTTGGATTGTTCTTCGAGCATGGAGTACGATGAAGAACATCTACAACGAAAGGAAGAGGCATGGATTGGCTTTAGAACTAGCCAAGTTTCTAATTGAAGCAGACTTTTCATGGGACGTCACACGACAAGCATCAAATAATGAAAGTTCGATTCTGACTGCTGGTGTAAACCCATTCCCAGATTCCAAAGATTCAGGTGATTTTGGTATCACTAGCCCTGAAGGCCCCCCGCCTCCATCCCTGAAAAAGAGTACTTCAATTATCCCATTGCACATTGCAACAGAGCATGGAATATTAGAAATTGTGGAGGAGACTCTTAGAACCCACCCTGCGATATTCGAGTATAGAAATATCGACAATCAAAACATACTGCACCTGTCCATTATGCACCGTCAGTGGGACATTTTTCATCGAGTAACAAAGATGGAGACACCTAAGTCGAGGTTGCAACGACAACTCGATGTTAATGACTTCACAGTATTGCACCAAGTTGGCAACATAGAGCATTACAATGGAGGCACCCAGCCTGGCCCTGCACTCCAATTGCAAGAAGAGCTGGAGTGGTTCGAGAAGGTGGAGGAGTTAATCCCACCACATTATAAAGAATTCCGTGCTTGTAAGAATGAGGAAGACACTGCCCTACAGTTTTTTTATAAAAGATATGTAGAGATTCTCAAGTGCTTGTCTGAGAACATTGACGTGGATAAGATAATACCACCCGATTCTCACAAGGAAGAAAAGAAACGGGAGACCCCCATAGAGTACTTTCGTCGCACACATGATCCGCTTCTCGAGAGGGCACAAGAATGGCTGAAGCGGACCTCCGAGTCGTGCTCGACGGTGGCCGGACTAATGGCCACCGTCGCCTTCGCAGCCGCCTTCACTGTCCCTGGAGGCAACGACGAGAACAGCGGCACTCCACTCCTTCTTCACAACCCTTTCTTCCTAGTCTTCATCATTACCGACGCTCTCTCCCTAGCCAGCTCGCTTACGTCACTCGTCATGTTCCTCTCCATCCTGACGTCACCGTTCGAGCTCCACGATTTCCGCCACTCTCTCCCTCGGAAGATGATCCTCGGCTTCACCTTCCTCTTCTTCTCGGTGGCGGTGACCATGCTGGCCTTTGCGTCCACACTGATGCTGACAATTCCAATGAAGAAGAGAGTGACCAAAAGTTTCGTGTACTGCGTTGCGTTTCTTCCCGTCACCATGTTTGCACTGTTGCAATTCCCGTTGTTGTTGGCCTTCAAAAAGAGTCTCAAGTACCCAGTCATGGCCATGAAAAGCGTATGGCGGTTTCTTATCTCTGCTCCTAATTCGAAAATTGCCAAGACCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

717

Amino Acids

81.44

Weight (kDa)

7.37

Isoelectric Point (pI)

50.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 53 - 142 4.5e-07 Ankyrin repeats (3 copies)
Ank_4 PF13637 90 - 139 1e-05 Ankyrin repeats (many copies)
PGG PF13962 540 - 652 1.1e-25 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1413, 1467
AasI GACNNNNNNGTC 2 cut(s) 1249, 1639
AatII GACGTC 2 cut(s) 933, 1838
AccB1I GGYRCC 2 cut(s) 325, 1331
AciI CCGC 8 cut(s) 1043, 1598, 1630, 1689, 1725, 1861, 1919, 2107
AclWI GGATC 2 cut(s) 1589, 1879
AcoI YGGCCR 4 cut(s) 391, 1656, 1668, 2088
AcsI RAATTY 4 cut(s) 402, 595, 718, 1418
AcuI CTGAAG 5 cut(s) 596, 618, 783, 1053, 1646
AcyI GRCGYC 3 cut(s) 605, 930, 1835
AfaI GTAC 8 cut(s) 16, 297, 496, 841, 1066, 1577, 1997, 2078
AfiI CCNNNNNNNGG 3 cut(s) 163, 1210, 2086
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 8 cut(s) 581, 601, 643, 743, 751, 911, 1071, 2062
AjiI CACGTC 1 cut(s) 1513
AjnI CCWGG 2 cut(s) 1339, 1702
AloI GAACNNNNNNTCC 2 cut(s) 249, 281
Alw21I GWGCWC 5 cut(s) 144, 470, 694, 1649, 1851
Alw26I GTCTC 5 cut(s) 48, 1118, 1235, 1556, 2075
Alw44I GTGCAC 1 cut(s) 140
AlwI GGATC 2 cut(s) 1589, 1879
AlwNI CAGNNNCTG 1 cut(s) 287
Ama87I CYCGRG 1 cut(s) 1604
AoxI GGCC 8 cut(s) 391, 1036, 1342, 1656, 1668, 1932, 2055, 2088
ApaLI GTGCAC 1 cut(s) 140
ApeKI GCWGC 6 cut(s) 278, 281, 380, 509, 761, 1685
ApoI RAATTY 4 cut(s) 402, 595, 718, 1418
AspS9I GGNCC 3 cut(s) 1037, 1343, 1632
AsuHPI GGTGA 7 cut(s) 71, 88, 1022, 1830, 1888, 1933, 2011
AsuII TTCGAA 1 cut(s) 2132
AvaI CYCGRG 1 cut(s) 1604
AvaII GGWCC 1 cut(s) 1632
BaeGI GKGCMC 2 cut(s) 144, 1615
BalI TGGCCA 3 cut(s) 393, 1670, 2090
BanI GGYRCC 2 cut(s) 325, 1331
BanII GRGCYC 1 cut(s) 1851
BarI GAAGNNNNNNTAC 2 cut(s) 839, 871
BbsI GAAGAC 4 cut(s) 608, 784, 1449, 1756
Bbv12I GWGCWC 5 cut(s) 144, 470, 694, 1649, 1851
BbvI GCAGC 6 cut(s) 265, 293, 392, 496, 748, 1697
BccI CCATC 3 cut(s) 1057, 1231, 1835
BceAI ACGGC 1 cut(s) 800
BciT130I CCWGG 2 cut(s) 1341, 1704
BclI TGATCA 1 cut(s) 666
BcoDI GTCTC 5 cut(s) 48, 1118, 1235, 1556, 2075
BfaI CTAG 5 cut(s) 552, 893, 1026, 1760, 1790
BfmI CTRYAG 2 cut(s) 279, 1454
BglI GCCNNNNNGGC 1 cut(s) 1035
BisI GCNGC 8 cut(s) 279, 282, 381, 510, 762, 1686, 1689, 1726
BlsI GCNGC 8 cut(s) 280, 283, 382, 511, 763, 1687, 1690, 1727
BmcAI AGTACT 2 cut(s) 1066, 1577
Bme1390I CCNGG 2 cut(s) 1341, 1704
Bme18I GGWCC 1 cut(s) 1632
BmeT110I CYCGRG 1 cut(s) 1604
BmgBI CACGTC 1 cut(s) 1513
BmgT120I GGNCC 3 cut(s) 1037, 1343, 1632
BmiI GGNNCC 4 cut(s) 327, 481, 1039, 1333
BmrFI CCNGG 2 cut(s) 1341, 1704
BmrI ACTGGG 2 cut(s) 2075, 2143
BmsI GCATC 5 cut(s) 97, 198, 694, 953, 1941
BmuI ACTGGG 2 cut(s) 2075, 2143
BpiI GAAGAC 4 cut(s) 608, 784, 1449, 1756
BplI GAGNNNNNCTC 2 cut(s) 1631, 1663
BpmI CTGGAG 2 cut(s) 1391, 1725
Bpu14I TTCGAA 1 cut(s) 2132
BpuEI CTTGAG 2 cut(s) 1472, 2057
Bsa29I ATCGAT 1 cut(s) 116
BsaAI YACGTR 2 cut(s) 14, 299
BsaBI GATNNNNATC 1 cut(s) 444
BsaHI GRCGYC 3 cut(s) 605, 930, 1835
BsaI GGTCTC 1 cut(s) 1556
BsaJI CCNNGG 3 cut(s) 1702, 1874, 1888
BsaWI WCCGGW 1 cut(s) 328
BsaXI ACNNNNNCTCC 4 cut(s) 299, 329, 1769, 1799
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 1210, 2086
Bse1I ACTGG 3 cut(s) 723, 2081, 2149
Bse3DI GCAATG 2 cut(s) 1079, 1086
Bse8I GATNNNNATC 1 cut(s) 444
BseBI CCWGG 2 cut(s) 1341, 1704
BseCI ATCGAT 1 cut(s) 116
BseDI CCNNGG 3 cut(s) 1702, 1874, 1888
BseGI GGATG 3 cut(s) 623, 1049, 1827
BseJI GATNNNNATC 1 cut(s) 444
BseLI CCNNNNNNNGG 3 cut(s) 163, 1210, 2086
BseMI GCAATG 2 cut(s) 1079, 1086
BseMII CTCAG 2 cut(s) 64, 1491
BseNI ACTGG 3 cut(s) 723, 2081, 2149
BseRI GAGGAG 3 cut(s) 608, 1136, 1406
BseSI GKGCMC 2 cut(s) 144, 1615
BseXI GCAGC 6 cut(s) 265, 293, 392, 496, 748, 1697
BseYI CCCAGC 1 cut(s) 1335
BsgI GTGCAG 2 cut(s) 1169, 1332
BshFI GGCC 8 cut(s) 393, 1038, 1344, 1658, 1670, 1934, 2057, 2090
BshNI GGYRCC 2 cut(s) 325, 1331
BshVI ATCGAT 1 cut(s) 116
BsiHKAI GWGCWC 5 cut(s) 144, 470, 694, 1649, 1851
BsiHKCI CYCGRG 1 cut(s) 1604
BsiSI CCGG 3 cut(s) 329, 560, 1659
BslFI GGGAC 4 cut(s) 441, 941, 1226, 1685
BslI CCNNNNNNNGG 3 cut(s) 163, 1210, 2086
BsmAI GTCTC 5 cut(s) 48, 1118, 1235, 1556, 2075
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 4 cut(s) 441, 941, 1226, 1685
BsmI GAATGC 1 cut(s) 638
BsnI GGCC 8 cut(s) 393, 1038, 1344, 1658, 1670, 1934, 2057, 2090
Bso31I GGTCTC 1 cut(s) 1556
BsoBI CYCGRG 1 cut(s) 1604
Bsp119I TTCGAA 1 cut(s) 2132
Bsp1286I GDGCHC 6 cut(s) 144, 470, 694, 1615, 1649, 1851
Bsp143I GATC 6 cut(s) 445, 666, 755, 809, 1594, 1884
BspACI CCGC 8 cut(s) 1043, 1598, 1630, 1689, 1725, 1861, 1919, 2107
BspANI GGCC 8 cut(s) 393, 1038, 1344, 1658, 1670, 1934, 2057, 2090
BspCNI CTCAG 2 cut(s) 63, 1492
BspDI ATCGAT 1 cut(s) 116
BspHI TCATGA 2 cut(s) 271, 709
BspLI GGNNCC 4 cut(s) 327, 481, 1039, 1333
BspMAI CTGCAG 1 cut(s) 283
BspPI GGATC 2 cut(s) 1589, 1879
BspQI GCTCTTC 1 cut(s) 1359
BspT104I TTCGAA 1 cut(s) 2132
BspT107I GGYRCC 2 cut(s) 325, 1331
BspTNI GGTCTC 1 cut(s) 1556
BsrDI GCAATG 2 cut(s) 1079, 1086
BsrI ACTGG 3 cut(s) 723, 2081, 2149
BssECI CCNNGG 3 cut(s) 1702, 1874, 1888
BssMI GATC 6 cut(s) 445, 666, 755, 809, 1594, 1884
BssNI GRCGYC 3 cut(s) 605, 930, 1835
Bst2UI CCWGG 2 cut(s) 1341, 1704
Bst6I CTCTTC 6 cut(s) 615, 802, 863, 1359, 1910, 1967
BstACI GRCGYC 3 cut(s) 605, 930, 1835
BstAPI GCANNNNNTGC 1 cut(s) 518
BstBAI YACGTR 2 cut(s) 14, 299
BstBI TTCGAA 1 cut(s) 2132
BstC8I GCNNGC 6 cut(s) 218, 417, 537, 1795, 1799, 1932
BstDEI CTNAG 4 cut(s) 50, 1130, 1248, 1500
BstEII GGTNACC 1 cut(s) 1921
BstF5I GGATG 3 cut(s) 623, 1049, 1827
BstKTI GATC 6 cut(s) 448, 669, 758, 812, 1597, 1887
BstMAI GTCTC 5 cut(s) 48, 1118, 1235, 1556, 2075
BstMBI GATC 6 cut(s) 445, 666, 755, 809, 1594, 1884
BstMWI GCNNNNNNNGC 6 cut(s) 518, 613, 1035, 1366, 1676, 1685
BstNI CCWGG 2 cut(s) 1341, 1704
BstNSI RCATGY 1 cut(s) 521
BstPI GGTNACC 1 cut(s) 1921
BstSCI CCNGG 2 cut(s) 1339, 1702
BstSFI CTRYAG 2 cut(s) 279, 1454
BstSLI GKGCMC 2 cut(s) 144, 1615
BstSNI TACGTA 1 cut(s) 14
BstV1I GCAGC 6 cut(s) 265, 293, 392, 496, 748, 1697
BstV2I GAAGAC 4 cut(s) 608, 784, 1449, 1756
Bsu15I ATCGAT 1 cut(s) 116
BsuRI GGCC 8 cut(s) 393, 1038, 1344, 1658, 1670, 1934, 2057, 2090
BsuTUI ATCGAT 1 cut(s) 116
BtgZI GCGATG 1 cut(s) 175
BtrI CACGTC 1 cut(s) 1513
BtsCI GGATG 3 cut(s) 623, 1049, 1827
BtsI GCAGTG 1 cut(s) 1446
BtsIMutI CAGTG 7 cut(s) 144, 730, 1214, 1446, 1695, 1946, 2030
Cac8I GCNNGC 6 cut(s) 218, 417, 537, 1795, 1799, 1932
CaiI CAGNNNCTG 1 cut(s) 287
CciI TCATGA 2 cut(s) 271, 709
Cfr13I GGNCC 3 cut(s) 1037, 1343, 1632
ClaI ATCGAT 1 cut(s) 116
CseI GACGC 3 cut(s) 613, 1787, 1929
Csp6I GTAC 8 cut(s) 15, 296, 495, 840, 1065, 1576, 1996, 2077
CviQI GTAC 8 cut(s) 15, 296, 495, 840, 1065, 1576, 1996, 2077
DdeI CTNAG 4 cut(s) 50, 1130, 1248, 1500
DpnI GATC 6 cut(s) 447, 668, 757, 811, 1596, 1886
DpnII GATC 6 cut(s) 445, 666, 755, 809, 1594, 1884
DrdI GACNNNNNNGTC 2 cut(s) 1249, 1639
DseDI GACNNNNNNGTC 2 cut(s) 1249, 1639
EaeI YGGCCR 4 cut(s) 391, 1656, 1668, 2088
Eam1104I CTCTTC 6 cut(s) 615, 802, 863, 1359, 1910, 1967
EarI CTCTTC 6 cut(s) 615, 802, 863, 1359, 1910, 1967
EciI GGCGGA 1 cut(s) 1850
Ecl136II GAGCTC 1 cut(s) 1849
Eco105I TACGTA 1 cut(s) 14
Eco24I GRGCYC 1 cut(s) 1851
Eco31I GGTCTC 1 cut(s) 1556
Eco32I GATATC 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 1632
Eco53kI GAGCTC 1 cut(s) 1849
Eco57I CTGAAG 5 cut(s) 596, 618, 783, 1053, 1646
Eco88I CYCGRG 1 cut(s) 1604
Eco91I GGTNACC 1 cut(s) 1921
EcoICRI GAGCTC 1 cut(s) 1849
EcoO109I RGGNCCY 1 cut(s) 1037
EcoO65I GGTNACC 1 cut(s) 1921
EcoRI GAATTC 1 cut(s) 1418
EcoRII CCWGG 2 cut(s) 1339, 1702
EcoRV GATATC 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 1851
Esp3I CGTCTC 1 cut(s) 48
FaqI GGGAC 4 cut(s) 441, 941, 1226, 1685
FauI CCCGC 1 cut(s) 1050
FauNDI CATATG 3 cut(s) 162, 488, 697
FbaI TGATCA 1 cut(s) 666
Fnu4HI GCNGC 8 cut(s) 279, 282, 381, 510, 762, 1686, 1689, 1726
FokI GGATG 3 cut(s) 610, 1036, 1814
FriOI GRGCYC 1 cut(s) 1851
Fsp4HI GCNGC 8 cut(s) 279, 282, 381, 510, 762, 1686, 1689, 1726
FspBI CTAG 5 cut(s) 552, 893, 1026, 1760, 1790
GluI GCNGC 8 cut(s) 279, 282, 381, 510, 762, 1686, 1689, 1726
GsaI CCCAGC 1 cut(s) 1339
GsuI CTGGAG 2 cut(s) 1391, 1725
HaeIII GGCC 8 cut(s) 393, 1038, 1344, 1658, 1670, 1934, 2057, 2090
HapII CCGG 3 cut(s) 329, 560, 1659
HgaI GACGC 3 cut(s) 613, 1787, 1929
Hin1I GRCGYC 3 cut(s) 605, 930, 1835
HindIII AAGCTT 1 cut(s) 81
HpaII CCGG 3 cut(s) 329, 560, 1659
HphI GGTGA 7 cut(s) 71, 88, 1022, 1830, 1888, 1933, 2011
Hpy166II GTNNAC 6 cut(s) 142, 296, 662, 982, 1944, 1996
Hpy188I TCNGA 6 cut(s) 22, 53, 969, 1501, 1639, 1878
Hpy188III TCNNGA 9 cut(s) 104, 272, 643, 670, 710, 1381, 1604, 1606, 1831
Hpy8I GTNNAC 6 cut(s) 142, 296, 662, 982, 1944, 1996
Hpy99I CGWCG 6 cut(s) 149, 1587, 1654, 1679, 1718, 1781
HpyAV CCTTC 8 cut(s) 350, 1028, 1378, 1690, 1702, 1748, 1909, 2068
HpyCH4IV ACGT 7 cut(s) 13, 63, 298, 930, 1512, 1805, 1835
HpyF10VI GCNNNNNNNGC 6 cut(s) 518, 613, 1035, 1366, 1676, 1685
HpyF3I CTNAG 4 cut(s) 50, 1130, 1248, 1500
HpySE526I ACGT 7 cut(s) 13, 63, 298, 930, 1512, 1805, 1835
Hsp92I GRCGYC 3 cut(s) 605, 930, 1835
Ksp22I TGATCA 1 cut(s) 666
Kzo9I GATC 6 cut(s) 445, 666, 755, 809, 1594, 1884
LguI GCTCTTC 1 cut(s) 1359
LmnI GCTCC 5 cut(s) 473, 621, 697, 1854, 2128
Lsp1109I GCAGC 6 cut(s) 265, 293, 392, 496, 748, 1697
LweI GCATC 5 cut(s) 97, 198, 694, 953, 1941
MaeI CTAG 5 cut(s) 552, 893, 1026, 1760, 1790
MaeII ACGT 7 cut(s) 13, 63, 298, 930, 1512, 1805, 1835
MaeIII GTNAC 8 cut(s) 59, 931, 1228, 1806, 1836, 1921, 1978, 2017
MalI GATC 6 cut(s) 447, 668, 757, 811, 1596, 1886
MboI GATC 6 cut(s) 445, 666, 755, 809, 1594, 1884
MfeI CAATTG 1 cut(s) 1355
MhlI GDGCHC 6 cut(s) 144, 470, 694, 1615, 1649, 1851
MlsI TGGCCA 3 cut(s) 393, 1670, 2090
MluNI TGGCCA 3 cut(s) 393, 1670, 2090
MlyI GAGTC 4 cut(s) 798, 1120, 1649, 2077
MmeI TCCRAC 2 cut(s) 158, 297
Mox20I TGGCCA 3 cut(s) 393, 1670, 2090
MscI TGGCCA 3 cut(s) 393, 1670, 2090
MseI TTAA 4 cut(s) 237, 804, 1278, 1397
MslI CAYNNNNRTG 5 cut(s) 1323, 1511, 1618, 1949, 2090
Msp20I TGGCCA 3 cut(s) 393, 1670, 2090
MspA1I CMGCKG 2 cut(s) 761, 1725
MspI CCGG 3 cut(s) 329, 560, 1659
MspR9I CCNGG 2 cut(s) 1341, 1704
MunI CAATTG 1 cut(s) 1355
Mva1269I GAATGC 1 cut(s) 638
MvaI CCWGG 2 cut(s) 1341, 1704
MwoI GCNNNNNNNGC 6 cut(s) 518, 613, 1035, 1366, 1676, 1685
NdeI CATATG 3 cut(s) 162, 488, 697
NdeII GATC 6 cut(s) 445, 666, 755, 809, 1594, 1884
NlaIV GGNNCC 4 cut(s) 327, 481, 1039, 1333
NmeAIII GCCGAG 2 cut(s) 547, 1869
NmuCI GTSAC 7 cut(s) 59, 931, 1806, 1836, 1921, 1978, 2017
NspI RCATGY 1 cut(s) 521
NspV TTCGAA 1 cut(s) 2132
PaeR7I CTCGAG 1 cut(s) 1604
PagI TCATGA 2 cut(s) 271, 709
PciSI GCTCTTC 1 cut(s) 1359
PcsI WCGNNNNNNNCGW 2 cut(s) 1851, 1998
PctI GAATGC 1 cut(s) 638
PfeI GAWTC 6 cut(s) 201, 964, 995, 1004, 1483, 1535
PkrI GCNGC 8 cut(s) 280, 283, 382, 511, 763, 1687, 1690, 1727
PleI GAGTC 4 cut(s) 797, 1120, 1648, 2076
PpsI GAGTC 4 cut(s) 797, 1120, 1648, 2076
Ppu21I YACGTR 2 cut(s) 14, 299
PsiI TTATAA 2 cut(s) 1413, 1467
Psp124BI GAGCTC 1 cut(s) 1851
Psp6I CCWGG 2 cut(s) 1339, 1702
PspEI GGTNACC 1 cut(s) 1921
PspFI CCCAGC 1 cut(s) 1335
PspGI CCWGG 2 cut(s) 1339, 1702
PspN4I GGNNCC 4 cut(s) 327, 481, 1039, 1333
PspPI GGNCC 3 cut(s) 1037, 1343, 1632
PstI CTGCAG 1 cut(s) 283
PstNI CAGNNNCTG 1 cut(s) 287
PvuII CAGCTG 1 cut(s) 761
RsaI GTAC 8 cut(s) 16, 297, 496, 841, 1066, 1577, 1997, 2078
RsaNI GTAC 8 cut(s) 15, 296, 495, 840, 1065, 1576, 1996, 2077
RseI CAYNNNNRTG 5 cut(s) 1323, 1511, 1618, 1949, 2090
SacI GAGCTC 1 cut(s) 1851
SapI GCTCTTC 1 cut(s) 1359
SaqAI TTAA 4 cut(s) 237, 804, 1278, 1397
SatI GCNGC 8 cut(s) 279, 282, 381, 510, 762, 1686, 1689, 1726
Sau3AI GATC 6 cut(s) 445, 666, 755, 809, 1594, 1884
Sau96I GGNCC 3 cut(s) 1037, 1343, 1632
ScaI AGTACT 2 cut(s) 1066, 1577
SchI GAGTC 4 cut(s) 798, 1120, 1649, 2077
ScrFI CCNGG 2 cut(s) 1341, 1704
SduI GDGCHC 6 cut(s) 144, 470, 694, 1615, 1649, 1851
SfaNI GCATC 5 cut(s) 97, 198, 694, 953, 1941
SfcI CTRYAG 2 cut(s) 279, 1454
Sfr274I CTCGAG 1 cut(s) 1604
SfuI TTCGAA 1 cut(s) 2132
SinI GGWCC 1 cut(s) 1632
SlaI CTCGAG 1 cut(s) 1604
SmiMI CAYNNNNRTG 5 cut(s) 1323, 1511, 1618, 1949, 2090
SmlI CTYRAG 3 cut(s) 1487, 1604, 2072
SmoI CTYRAG 3 cut(s) 1487, 1604, 2072
SnaBI TACGTA 1 cut(s) 14
SsiI CCGC 8 cut(s) 1043, 1598, 1630, 1689, 1725, 1861, 1919, 2107
SspI AATATT 1 cut(s) 1107
SspMI CTAG 5 cut(s) 552, 893, 1026, 1760, 1790
SstI GAGCTC 1 cut(s) 1851
StyD4I CCNGG 2 cut(s) 1339, 1702
TaiI ACGT 7 cut(s) 16, 66, 301, 933, 1515, 1808, 1838
TatI WGTACW 3 cut(s) 1064, 1575, 1995
TauI GCSGC 2 cut(s) 1691, 1728
TfiI GAWTC 6 cut(s) 201, 964, 995, 1004, 1483, 1535
Tru1I TTAA 4 cut(s) 237, 804, 1278, 1397
Tru9I TTAA 4 cut(s) 237, 804, 1278, 1397
TscAI CASTG 7 cut(s) 144, 730, 1214, 1453, 1702, 1953, 2037
TseFI GTSAC 7 cut(s) 59, 931, 1806, 1836, 1921, 1978, 2017
TseI GCWGC 6 cut(s) 278, 281, 380, 509, 761, 1685
Tsp45I GTSAC 7 cut(s) 59, 931, 1806, 1836, 1921, 1978, 2017
TspGWI ACGGA 1 cut(s) 1412
TspRI CASTG 7 cut(s) 144, 730, 1214, 1453, 1702, 1953, 2037
VneI GTGCAC 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 1632
XapI RAATTY 4 cut(s) 402, 595, 718, 1418
XceI RCATGY 1 cut(s) 521
XhoI CTCGAG 1 cut(s) 1604
XspI CTAG 5 cut(s) 552, 893, 1026, 1760, 1790
ZraI GACGTC 2 cut(s) 931, 1836
ZrmI AGTACT 2 cut(s) 1066, 1577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.