MD05G1354800.v1.1

Ankyrin repeat-containing protein At3g12360-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000724
Physical Location & Seq
Forward (+)
47115805 .. 47119487
3683 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1354800.v1.1.491

Sequence Viewer

Length: 2601 bp
ATGGAGCACTGGGAAACGCTAACGGATAGGCCGGTGGAGGAGGATGAGGTAGCGAGAGAGGTTAGCTTAATTCACATTGAAGAATTGAAAGATCCCTATGAATATGTCATGACGGAGAACTGGGAAGGCGTGCAGGATTTCTTCGAGAAGAATCCGGAACATCTGCTCTCTAAAATTACAATGGACGGAGGCACTGTATTCCACCTTGCGGCTTCTTGCAGCAGCAAATCACAACGCAAAAATCTCAAAATGGTTATCGATATACTCCGCAGGAATCCATCCGACGGTGACGTGAGAAGAGCTCTGACGAAGAAAAATAATGCCGGAAACAATACTCTCCATGAGGTGAGTATGACCGGCAACAAGGAAGCGGCAATGTACTTGGTGAGCATTGCTGATAAAGAGAAATGTGCACTGGAGCTGCTGGAGACTCGGAACTCTTCAGGAGAAACTTCGCTCTTCAAGGCGGCTGCTTACGGTTTCACTGACTTGGTCAAGTTTTATGTTGGCAAACTGGAGAATTATGATCGGGACAACCTTTGGAAGCACTTCCACAGAAGTGACAAAACGTCCATTCTTCATATCGCAGTCGTTGCGCAACATTTCGAGACCGCTCTTTGGTTAGTGGAGAACAACCCATATCTAGGGCCCCTAAAGAACAATAAAGGATTGACAAGCCTTCAGTTGCTAGCCCAAATGCCAACTGCCTTTGATCCACATTTTGAAAAATGCAAATGGAAGATGCTAATTTATTATTGCCTTCCTGTTGGAGGAGATGTGGTCACACCAAATCAGAATATGAAGGATAAGGATGATGTGGAGAGCAATAGGAAGGATGATGTGGAGAGCAATATGAAGGATGATGTGGAGAGCAATAGGAAGGATGATGTGGAGAGCAATAGGAAGCATAAGGATAAGGATGATGTGGAGAGCAATATGAAGGATGATGTGGAGAGCAATAGGAAGGATGATGTGGAGAGCAATATGAAGGATGATGTGGAGAGCAATAGGAAGGATGATGTGGAGAGCAATAGGAAGCATAGGGATGATATGGAGAAGCATAAGGATGATATGGATGATATGGAGAAGCATAAGGATGATGTGGAGAGCAATAGGAAGGATGATGTGGAGAGCAGCATGGAGAGCAACCAGCCGCACCGCCAATCCATCTTTCGGAACAAGCTTGCAGGCTTTGTGAAGGTTTACATTTCACTACGGGACTCCCTTGCTAAAAGTTTGCTTACGTTCTGGCTTTTTTTCGAAATAGGAGGCCTGATATGGGAGGACAAGAAAAATAAAAAAGCACTAGATAAACTCATCCCACTGCTCGCCAAGTGGGACAAATCTTGGTGTACTATAGATGCTGCAGGCGAAGATAGCACTTCTATGCTGTCAGAGAAAAAAGGTTATGATAACGGAGGCGAAAAAAGAAAGGCGGCCGTATCGGATGAGACAAATGGTGATGATGGAGGTGGAAAAGAAAAGGATGCAGCCAAATCGGAAAAACTTAATAAAGATGGTGATGACGGAGGAAAAAAAGGACAGGCGGCCGCATCGGATAAGACAAATGGTGATGATGGAGGTGGAAAAAAAGAGGAATTAGCCAAATCGAAAGAGGAGGCGAGGAATTCTAGCGCATTGCTTATGGCAACTATCAATGGAATTCTACCCATTGTGAAGGAGATAGTTGGCCAGCATCCTCAGGCAATCGAGCATGTTAACTATAACATACGCAACGTTTTGCATCTGGCCATTAAGTACCGTCGGAAAGAGATCCTTAATCATATCCAAAGCTTACCACATGTGATGCCGAAGCTGAGTAAGAGGATAGACAAATATGGAAACACCATATTGCACCAGGCTGCAGATAGGAGTTACTACTCCATATCATTGTCTCAGAAATTAATAGGCCCTGCCATGCAATTGCAGGATGAGCAGCACTGGATGCTGCGTGTACAAGAGATGATACCGCCTCATTACACTCTTCACCACAACAACAAGGATCAGACAGCGGAGGAGCTGTTCAACCAAGAGCATGACAAACTTCTGGAGGAAGCACAAAAATGGGTAAAAGAAACGGCTCAGTCATGCTCAACCGTGGCGGTGCTAGTGGCCACTGTGGTCTTTGCAGCCGCCTATGCCATTCCTGGGGGTTTTAACGACCAAAGCGGCCGTCCTATTTTCCAGGACAATCCTCTCTTTTTGCTCTTCACCTGCATGGACGTTGTGGCCATTTCCTGCTCACTATCTTCTGTGGCATTCTTTCTCTCTGTCCTCTCCTCCCCTCTCGAGTACCCACTTTTCGTTAACAGCATTCCTCGCAAGGTCATGGCAGGATTCATCTTGCTCTTCTTGTCCATGGCAACCACCATGCTCGCCTTTGCTGCCACCATTTTGCTCGTGATTCGCGTCAAGAAGAAATGGACCATGTCTATACTTTACCCTATTGCCTTTTTCCCCGTCCCTCTATTCGCCTTGCTTCAGTTTCCTATGTATCAATCCTTCATGGTCATGCTTAAGACAATTAATGCTTGGGTTTTCAAGGTTTTCAAACCGTTATTGGCCTTTCTCCGCTTTCATGGCTCTAGGCGGATCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

867

Amino Acids

98.55

Weight (kDa)

6.18

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 688 - 800 2.2e-22 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2222
Acc16I TGCGCA 1 cut(s) 597
Acc36I ACCTGC 1 cut(s) 2222
AccBSI CCGCTC 1 cut(s) 614
AccII CGCG 1 cut(s) 2409
AccIII TCCGGA 1 cut(s) 154
AclI AACGTT 1 cut(s) 1737
AclWI GGATC 5 cut(s) 86, 707, 1768, 2010, 2600
AcoI YGGCCR 7 cut(s) 1437, 1548, 1690, 1749, 2112, 2170, 2229
AcsI RAATTY 2 cut(s) 1627, 1662
AcuI CTGAAG 3 cut(s) 426, 665, 2465
AfaI GTAC 5 cut(s) 380, 1354, 1760, 1956, 2294
AfiI CCNNNNNNNGG 8 cut(s) 208, 284, 618, 644, 770, 1173, 1279, 2148
AflII CTTAAG 1 cut(s) 2516
AflIII ACRYGT 1 cut(s) 1801
AgsI TTSAA 7 cut(s) 80, 88, 463, 725, 2026, 2542, 2551
AhdI GACNNNNNGTC 1 cut(s) 568
AjiI CACGTC 1 cut(s) 292
AjnI CCWGG 3 cut(s) 1857, 2146, 2184
AjuI GAANNNNNNNTTGG 2 cut(s) 1155, 1187
AleI CACNNNNGTG 1 cut(s) 558
AloI GAACNNNNNNTCC 2 cut(s) 2006, 2038
AluBI AGCT 7 cut(s) 66, 302, 421, 1183, 1794, 1816, 2020
AluI AGCT 7 cut(s) 66, 302, 421, 1183, 1794, 1816, 2020
Alw21I GWGCWC 3 cut(s) 9, 304, 415
Alw26I GTCTC 4 cut(s) 422, 602, 1445, 1899
Alw44I GTGCAC 1 cut(s) 411
AlwI GGATC 5 cut(s) 86, 707, 1768, 2010, 2600
Ama87I CYCGRG 1 cut(s) 2288
Aor13HI TCCGGA 1 cut(s) 154
ApaI GGGCCC 1 cut(s) 651
ApaLI GTGCAC 1 cut(s) 411
ApoI RAATTY 2 cut(s) 1627, 1662
ArsI GACNNNNNNTTYG 2 cut(s) 2158, 2190
AseI ATTAAT 2 cut(s) 1904, 2526
Asp700I GAANNNNTTC 1 cut(s) 548
AspLEI GCGC 2 cut(s) 598, 1637
AspS9I GGNCC 4 cut(s) 647, 648, 1910, 2424
AsuHPI GGTGA 8 cut(s) 299, 358, 397, 1472, 1532, 1583, 1979, 2203
AsuII TTCGAA 1 cut(s) 1260
AsuNHI GCTAGC 1 cut(s) 688
AvaI CYCGRG 1 cut(s) 2288
AvaII GGWCC 1 cut(s) 2424
AxyI CCTNAGG 1 cut(s) 1701
BaeGI GKGCMC 2 cut(s) 415, 651
BalI TGGCCA 4 cut(s) 1692, 1751, 2114, 2231
BanII GRGCYC 2 cut(s) 304, 651
BarI GAAGNNNNNNTAC 2 cut(s) 2486, 2518
BauI CACGAG 1 cut(s) 2399
Bbv12I GWGCWC 3 cut(s) 9, 304, 415
BccI CCATC 5 cut(s) 286, 1175, 1460, 1511, 1571
BceAI ACGGC 3 cut(s) 1424, 2094, 2157
BcgI CGANNNNNNTGC 2 cut(s) 1479, 1513
BciT130I CCWGG 3 cut(s) 1859, 2148, 2186
BcoDI GTCTC 4 cut(s) 422, 602, 1445, 1899
BfaI CTAG 6 cut(s) 644, 689, 1307, 1632, 2108, 2586
BfmI CTRYAG 3 cut(s) 1356, 1365, 1863
BfrI CTTAAG 1 cut(s) 2516
BfuAI ACCTGC 1 cut(s) 2222
Bme1390I CCNGG 3 cut(s) 1859, 2148, 2186
Bme18I GGWCC 1 cut(s) 2424
BmeRI GACNNNNNGTC 1 cut(s) 568
BmeT110I CYCGRG 1 cut(s) 2288
BmgBI CACGTC 1 cut(s) 292
BmgT120I GGNCC 4 cut(s) 647, 648, 1910, 2424
BmiI GGNNCC 2 cut(s) 649, 650
BmrFI CCNGG 3 cut(s) 1859, 2148, 2186
BmrI ACTGGG 2 cut(s) 19, 130
BmsI GCATC 8 cut(s) 732, 1351, 1477, 1562, 1705, 1753, 1797, 1935
BmtI GCTAGC 1 cut(s) 692
BmuI ACTGGG 2 cut(s) 19, 130
BplI GAGNNNNNCTC 2 cut(s) 286, 318
BpmI CTGGAG 4 cut(s) 437, 446, 536, 2069
Bpu14I TTCGAA 1 cut(s) 1260
Bsa29I ATCGAT 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 602
BsaJI CCNNGG 3 cut(s) 2097, 2147, 2358
BsaWI WCCGGW 1 cut(s) 154
BsaXI ACNNNNNCTCC 8 cut(s) 180, 210, 762, 765, 792, 795, 2006, 2036
Bsc4I CCNNNNNNNGG 8 cut(s) 208, 284, 618, 644, 770, 1173, 1279, 2148
Bse118I RCCGGY 2 cut(s) 31, 356
Bse1I ACTGG 5 cut(s) 14, 125, 420, 519, 1946
Bse21I CCTNAGG 1 cut(s) 1701
Bse3DI GCAATG 3 cut(s) 381, 390, 1637
BseAI TCCGGA 1 cut(s) 154
BseBI CCWGG 3 cut(s) 1859, 2148, 2186
BseCI ATCGAT 1 cut(s) 258
BseDI CCNNGG 3 cut(s) 2097, 2147, 2358
BseLI CCNNNNNNNGG 8 cut(s) 208, 284, 618, 644, 770, 1173, 1279, 2148
BseMI GCAATG 3 cut(s) 381, 390, 1637
BseMII CTCAG 4 cut(s) 1715, 1808, 1910, 2096
BseNI ACTGG 5 cut(s) 14, 125, 420, 519, 1946
BseRI GAGGAG 5 cut(s) 53, 786, 1631, 2030, 2269
BseSI GKGCMC 2 cut(s) 415, 651
BseX3I CGGCCG 3 cut(s) 1437, 1548, 2170
BsgI GTGCAG 1 cut(s) 152
Bsh1236I CGCG 1 cut(s) 2409
Bsh1285I CGRYCG 3 cut(s) 1440, 1551, 2173
BshVI ATCGAT 1 cut(s) 258
BsiEI CGRYCG 3 cut(s) 1440, 1551, 2173
BsiHKAI GWGCWC 3 cut(s) 9, 304, 415
BsiHKCI CYCGRG 1 cut(s) 2288
BsiSI CCGG 4 cut(s) 32, 155, 324, 357
BslFI GGGAC 4 cut(s) 545, 1232, 1352, 2447
BslI CCNNNNNNNGG 8 cut(s) 208, 284, 618, 644, 770, 1173, 1279, 2148
BsmAI GTCTC 4 cut(s) 422, 602, 1445, 1899
BsmFI GGGAC 4 cut(s) 545, 1232, 1352, 2447
BsmI GAATGC 2 cut(s) 2258, 2313
Bso31I GGTCTC 1 cut(s) 602
BsoBI CYCGRG 1 cut(s) 2288
Bsp119I TTCGAA 1 cut(s) 1260
Bsp120I GGGCCC 1 cut(s) 647
Bsp1286I GDGCHC 4 cut(s) 9, 304, 415, 651
Bsp13I TCCGGA 1 cut(s) 154
Bsp1407I TGTACA 1 cut(s) 1954
Bsp143I GATC 6 cut(s) 91, 526, 712, 1773, 2002, 2592
Bsp19I CCATGG 1 cut(s) 2358
BspCNI CTCAG 4 cut(s) 1714, 1809, 1909, 2095
BspDI ATCGAT 1 cut(s) 258
BspEI TCCGGA 1 cut(s) 154
BspFNI CGCG 1 cut(s) 2409
BspHI TCATGA 1 cut(s) 108
BspLI GGNNCC 2 cut(s) 649, 650
BspMAI CTGCAG 2 cut(s) 1369, 1867
BspMI ACCTGC 1 cut(s) 2222
BspOI GCTAGC 1 cut(s) 692
BspPI GGATC 5 cut(s) 86, 707, 1768, 2010, 2600
BspQI GCTCTTC 4 cut(s) 292, 464, 2213, 2354
BspT104I TTCGAA 1 cut(s) 1260
BspTI CTTAAG 1 cut(s) 2516
BspTNI GGTCTC 1 cut(s) 602
BsrBI CCGCTC 1 cut(s) 614
BsrDI GCAATG 3 cut(s) 381, 390, 1637
BsrFI RCCGGY 2 cut(s) 31, 356
BsrGI TGTACA 1 cut(s) 1954
BsrI ACTGG 5 cut(s) 14, 125, 420, 519, 1946
BssAI RCCGGY 2 cut(s) 31, 356
BssECI CCNNGG 3 cut(s) 2097, 2147, 2358
BssMI GATC 6 cut(s) 91, 526, 712, 1773, 2002, 2592
BssSI CACGAG 1 cut(s) 2399
BssT1I CCWWGG 1 cut(s) 2358
Bst2BI CACGAG 1 cut(s) 2399
Bst2UI CCWGG 3 cut(s) 1859, 2148, 2186
Bst4CI ACNGT 7 cut(s) 196, 287, 479, 1763, 2098, 2119, 2556
Bst6I CTCTTC 6 cut(s) 292, 445, 464, 1989, 2213, 2354
BstAFI CTTAAG 1 cut(s) 2516
BstAPI GCANNNNNTGC 2 cut(s) 593, 1945
BstAUI TGTACA 1 cut(s) 1954
BstBI TTCGAA 1 cut(s) 1260
BstC8I GCNNGC 8 cut(s) 131, 690, 1185, 1189, 1329, 1369, 1694, 2376
BstDEI CTNAG 4 cut(s) 1701, 1817, 1896, 2082
BstDSI CCRYGG 2 cut(s) 2097, 2358
BstENI CCTNNNNNAGG 1 cut(s) 768
BstFNI CGCG 1 cut(s) 2409
BstHHI GCGC 2 cut(s) 598, 1637
BstKTI GATC 6 cut(s) 94, 529, 715, 1776, 2005, 2595
BstMAI GTCTC 4 cut(s) 422, 602, 1445, 1899
BstMBI GATC 6 cut(s) 91, 526, 712, 1773, 2002, 2592
BstMCI CGRYCG 3 cut(s) 1440, 1551, 2173
BstMWI GCNNNNNNNGC 9 cut(s) 593, 1143, 1377, 1933, 1945, 2138, 2319, 2384, 2580
BstNI CCWGG 3 cut(s) 1859, 2148, 2186
BstNSI RCATGY 2 cut(s) 1718, 1805
BstSCI CCNGG 3 cut(s) 1857, 2146, 2184
BstSFI CTRYAG 3 cut(s) 1356, 1365, 1863
BstSLI GKGCMC 2 cut(s) 415, 651
BstUI CGCG 1 cut(s) 2409
BstX2I RGATCY 2 cut(s) 91, 1773
BstYI RGATCY 2 cut(s) 91, 1773
BstZI CGGCCG 3 cut(s) 1437, 1548, 2170
Bsu15I ATCGAT 1 cut(s) 258
Bsu36I CCTNAGG 1 cut(s) 1701
BsuTUI ATCGAT 1 cut(s) 258
BtgI CCRYGG 2 cut(s) 2097, 2358
BtrI CACGTC 1 cut(s) 292
BtsI GCAGTG 1 cut(s) 1322
BtsIMutI CAGTG 7 cut(s) 7, 192, 413, 483, 1322, 1939, 2115
BveI ACCTGC 1 cut(s) 2222
Cac8I GCNNGC 8 cut(s) 131, 690, 1185, 1189, 1329, 1369, 1694, 2376
CciI TCATGA 1 cut(s) 108
CciNI GCGGCCGC 1 cut(s) 1548
CfoI GCGC 2 cut(s) 598, 1637
Cfr10I RCCGGY 2 cut(s) 31, 356
Cfr13I GGNCC 4 cut(s) 647, 648, 1910, 2424
ClaI ATCGAT 1 cut(s) 258
CseI GACGC 1 cut(s) 2398
Csp6I GTAC 5 cut(s) 379, 1353, 1759, 1955, 2293
CviQI GTAC 5 cut(s) 379, 1353, 1759, 1955, 2293
DdeI CTNAG 4 cut(s) 1701, 1817, 1896, 2082
DpnI GATC 6 cut(s) 93, 528, 714, 1775, 2004, 2594
DpnII GATC 6 cut(s) 91, 526, 712, 1773, 2002, 2592
DriI GACNNNNNGTC 1 cut(s) 568
EaeI YGGCCR 7 cut(s) 1437, 1548, 1690, 1749, 2112, 2170, 2229
EagI CGGCCG 3 cut(s) 1437, 1548, 2170
Eam1104I CTCTTC 6 cut(s) 292, 445, 464, 1989, 2213, 2354
Eam1105I GACNNNNNGTC 1 cut(s) 568
EarI CTCTTC 6 cut(s) 292, 445, 464, 1989, 2213, 2354
Ecl136II GAGCTC 1 cut(s) 302
EclXI CGGCCG 3 cut(s) 1437, 1548, 2170
Eco130I CCWWGG 1 cut(s) 2358
Eco147I AGGCCT 1 cut(s) 1272
Eco24I GRGCYC 2 cut(s) 304, 651
Eco31I GGTCTC 1 cut(s) 602
Eco47I GGWCC 1 cut(s) 2424
Eco52I CGGCCG 3 cut(s) 1437, 1548, 2170
Eco53kI GAGCTC 1 cut(s) 302
Eco57I CTGAAG 3 cut(s) 426, 665, 2465
Eco81I CCTNAGG 1 cut(s) 1701
Eco88I CYCGRG 1 cut(s) 2288
EcoICRI GAGCTC 1 cut(s) 302
EcoNI CCTNNNNNAGG 1 cut(s) 768
EcoO109I RGGNCCY 3 cut(s) 647, 648, 1910
EcoRI GAATTC 2 cut(s) 1627, 1662
EcoRII CCWGG 3 cut(s) 1857, 2146, 2184
EcoT14I CCWWGG 1 cut(s) 2358
EcoT38I GRGCYC 2 cut(s) 304, 651
ErhI CCWWGG 1 cut(s) 2358
FalI AAGNNNNNCTT 2 cut(s) 1761, 1793
FaqI GGGAC 4 cut(s) 545, 1232, 1352, 2447
FriOI GRGCYC 2 cut(s) 304, 651
FspBI CTAG 6 cut(s) 644, 689, 1307, 1632, 2108, 2586
FspI TGCGCA 1 cut(s) 597
GlaI GCGC 2 cut(s) 597, 1636
GsuI CTGGAG 4 cut(s) 437, 446, 536, 2069
HapII CCGG 4 cut(s) 32, 155, 324, 357
HgaI GACGC 1 cut(s) 2398
HhaI GCGC 2 cut(s) 598, 1637
Hin6I GCGC 2 cut(s) 596, 1635
HinP1I GCGC 2 cut(s) 596, 1635
HincII GTYRAC 2 cut(s) 1720, 2308
HindII GTYRAC 2 cut(s) 1720, 2308
HindIII AAGCTT 2 cut(s) 1181, 1792
HinfI GANTC 6 cut(s) 151, 274, 430, 1220, 2337, 2404
HpaI GTTAAC 2 cut(s) 1720, 2308
HpaII CCGG 4 cut(s) 32, 155, 324, 357
HphI GGTGA 8 cut(s) 299, 358, 397, 1472, 1532, 1583, 1979, 2203
Hpy166II GTNNAC 6 cut(s) 413, 1204, 1353, 1720, 1955, 2308
Hpy8I GTNNAC 6 cut(s) 413, 1204, 1353, 1720, 1955, 2308
Hpy99I CGWCG 2 cut(s) 287, 1767
HpyCH4III ACNGT 7 cut(s) 196, 287, 479, 1763, 2098, 2119, 2556
HpyCH4IV ACGT 5 cut(s) 291, 569, 1244, 1737, 2223
HpyF10VI GCNNNNNNNGC 9 cut(s) 593, 1143, 1377, 1933, 1945, 2138, 2319, 2384, 2580
HpyF3I CTNAG 4 cut(s) 1701, 1817, 1896, 2082
HpySE526I ACGT 5 cut(s) 291, 569, 1244, 1737, 2223
HspAI GCGC 2 cut(s) 596, 1635
Kpn2I TCCGGA 1 cut(s) 154
KspAI GTTAAC 2 cut(s) 1720, 2308
Kzo9I GATC 6 cut(s) 91, 526, 712, 1773, 2002, 2592
LguI GCTCTTC 4 cut(s) 292, 464, 2213, 2354
LmnI GCTCC 3 cut(s) 4, 418, 2017
LweI GCATC 8 cut(s) 732, 1351, 1477, 1562, 1705, 1753, 1797, 1935
MaeI CTAG 6 cut(s) 644, 689, 1307, 1632, 2108, 2586
MaeII ACGT 5 cut(s) 291, 569, 1244, 1737, 2223
MaeIII GTNAC 4 cut(s) 287, 560, 781, 1874
MalI GATC 6 cut(s) 93, 528, 714, 1775, 2004, 2594
MbiI CCGCTC 1 cut(s) 614
MboI GATC 6 cut(s) 91, 526, 712, 1773, 2002, 2592
MfeI CAATTG 1 cut(s) 1922
MflI RGATCY 2 cut(s) 91, 1773
MhlI GDGCHC 4 cut(s) 9, 304, 415, 651
MlsI TGGCCA 4 cut(s) 1692, 1751, 2114, 2231
MluNI TGGCCA 4 cut(s) 1692, 1751, 2114, 2231
MlyI GAGTC 2 cut(s) 424, 1214
MmeI TCCRAC 3 cut(s) 306, 748, 1745
Mox20I TGGCCA 4 cut(s) 1692, 1751, 2114, 2231
MroI TCCGGA 1 cut(s) 154
MroXI GAANNNNTTC 1 cut(s) 548
MscI TGGCCA 4 cut(s) 1692, 1751, 2114, 2231
MslI CAYNNNNRTG 8 cut(s) 558, 1044, 1065, 1095, 1385, 2062, 2216, 2510
Msp20I TGGCCA 4 cut(s) 1692, 1751, 2114, 2231
MspA1I CMGCKG 1 cut(s) 2012
MspCI CTTAAG 1 cut(s) 2516
MspI CCGG 4 cut(s) 32, 155, 324, 357
MspR9I CCNGG 3 cut(s) 1859, 2148, 2186
MunI CAATTG 1 cut(s) 1922
Mva1269I GAATGC 2 cut(s) 2258, 2313
MvaI CCWGG 3 cut(s) 1859, 2148, 2186
MvnI CGCG 1 cut(s) 2409
MwoI GCNNNNNNNGC 9 cut(s) 593, 1143, 1377, 1933, 1945, 2138, 2319, 2384, 2580
NcoI CCATGG 1 cut(s) 2358
NdeII GATC 6 cut(s) 91, 526, 712, 1773, 2002, 2592
NheI GCTAGC 1 cut(s) 688
NlaIV GGNNCC 2 cut(s) 649, 650
NmuCI GTSAC 3 cut(s) 287, 560, 781
NotI GCGGCCGC 1 cut(s) 1548
NsbI TGCGCA 1 cut(s) 597
NspI RCATGY 2 cut(s) 1718, 1805
NspV TTCGAA 1 cut(s) 1260
OliI CACNNNNGTG 1 cut(s) 558
PaeR7I CTCGAG 1 cut(s) 2288
PagI TCATGA 1 cut(s) 108
PaqCI CACCTGC 1 cut(s) 2222
PceI AGGCCT 1 cut(s) 1272
PciI ACATGT 1 cut(s) 1801
PciSI GCTCTTC 4 cut(s) 292, 464, 2213, 2354
PcsI WCGNNNNNNNCGW 1 cut(s) 2406
PctI GAATGC 2 cut(s) 2258, 2313
PdmI GAANNNNTTC 1 cut(s) 548
PfeI GAWTC 4 cut(s) 151, 274, 2337, 2404
PflFI GACNNNGTC 2 cut(s) 491, 2428
PfoI TCCNGGA 1 cut(s) 2184
PleI GAGTC 2 cut(s) 424, 1214
PpsI GAGTC 2 cut(s) 424, 1214
PscI ACATGT 1 cut(s) 1801
PshBI ATTAAT 2 cut(s) 1904, 2526
Psp124BI GAGCTC 1 cut(s) 304
Psp1406I AACGTT 1 cut(s) 1737
Psp6I CCWGG 3 cut(s) 1857, 2146, 2184
PspGI CCWGG 3 cut(s) 1857, 2146, 2184
PspN4I GGNNCC 2 cut(s) 649, 650
PspOMI GGGCCC 1 cut(s) 647
PspPI GGNCC 4 cut(s) 647, 648, 1910, 2424
PstI CTGCAG 2 cut(s) 1369, 1867
PsuI RGATCY 2 cut(s) 91, 1773
PsyI GACNNNGTC 2 cut(s) 491, 2428
RsaI GTAC 5 cut(s) 380, 1354, 1760, 1956, 2294
RsaNI GTAC 5 cut(s) 379, 1353, 1759, 1955, 2293
RseI CAYNNNNRTG 8 cut(s) 558, 1044, 1065, 1095, 1385, 2062, 2216, 2510
SacI GAGCTC 1 cut(s) 304
SapI GCTCTTC 4 cut(s) 292, 464, 2213, 2354
Sau3AI GATC 6 cut(s) 91, 526, 712, 1773, 2002, 2592
Sau96I GGNCC 4 cut(s) 647, 648, 1910, 2424
SchI GAGTC 2 cut(s) 424, 1214
ScrFI CCNGG 3 cut(s) 1859, 2148, 2186
SduI GDGCHC 4 cut(s) 9, 304, 415, 651
SfaNI GCATC 8 cut(s) 732, 1351, 1477, 1562, 1705, 1753, 1797, 1935
SfcI CTRYAG 3 cut(s) 1356, 1365, 1863
Sfr274I CTCGAG 1 cut(s) 2288
SfuI TTCGAA 1 cut(s) 1260
SinI GGWCC 1 cut(s) 2424
SlaI CTCGAG 1 cut(s) 2288
SmiMI CAYNNNNRTG 8 cut(s) 558, 1044, 1065, 1095, 1385, 2062, 2216, 2510
SmlI CTYRAG 2 cut(s) 2288, 2516
SmoI CTYRAG 2 cut(s) 2288, 2516
SseBI AGGCCT 1 cut(s) 1272
SspMI CTAG 6 cut(s) 644, 689, 1307, 1632, 2108, 2586
SstI GAGCTC 1 cut(s) 304
StuI AGGCCT 1 cut(s) 1272
StyD4I CCNGG 3 cut(s) 1857, 2146, 2184
StyI CCWWGG 1 cut(s) 2358
TaaI ACNGT 7 cut(s) 196, 287, 479, 1763, 2098, 2119, 2556
TaiI ACGT 5 cut(s) 294, 572, 1247, 1740, 2226
TaqI TCGA 7 cut(s) 144, 258, 606, 1260, 1610, 1710, 2289
TatI WGTACW 3 cut(s) 378, 1352, 1954
TauI GCSGC 9 cut(s) 212, 374, 470, 1156, 1439, 1550, 1553, 2135, 2172
TfiI GAWTC 4 cut(s) 151, 274, 2337, 2404
TscAI CASTG 7 cut(s) 14, 199, 420, 490, 1329, 1946, 2122
TseFI GTSAC 3 cut(s) 287, 560, 781
Tsp45I GTSAC 3 cut(s) 287, 560, 781
TspDTI ATGAA 9 cut(s) 114, 569, 815, 869, 953, 1001, 2329, 2494, 2567
TspGWI ACGGA 5 cut(s) 38, 128, 201, 1431, 1542
TspRI CASTG 7 cut(s) 14, 199, 420, 490, 1329, 1946, 2122
Tth111I GACNNNGTC 2 cut(s) 491, 2428
Vha464I CTTAAG 1 cut(s) 2516
VneI GTGCAC 1 cut(s) 411
VpaK11BI GGWCC 1 cut(s) 2424
VspI ATTAAT 2 cut(s) 1904, 2526
XagI CCTNNNNNAGG 1 cut(s) 768
XapI RAATTY 2 cut(s) 1627, 1662
XceI RCATGY 2 cut(s) 1718, 1805
XhoI CTCGAG 1 cut(s) 2288
XmnI GAANNNNTTC 1 cut(s) 548
XspI CTAG 6 cut(s) 644, 689, 1307, 1632, 2108, 2586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.