Rorug04G0394000

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000724
Physical Location & Seq
Forward (+)
56042252 .. 56044898
2647 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0394000.1

Sequence Viewer

Length: 1269 bp
ATGTCAACATCATACCTCCCAGCAACAACGGATTCAATTGCTCTGGCTTTAGAGGCCAAAGCTCCATCTGAAGCCATAGCTATTCTGTATCGTATTCTTGAAAACCAATCCACTTCTTCCGAAGCTCTACGCATAAAGGAACAGGCCATCACCAATCTGTCAGATCTTCTTAGACAAGAGAATCGGGGAGAAGATCTTCGTAACCTTCTTACCCAGTTGAGGCCTTTCTTTAGCTTGATTCCAAAGGCAAAAACTGCAAAGATTGTTCGTGGCATAATAGATACAGTAGCTAAAATACCCGGCACATCTGAACTTCAGATTTCCCTTTGCAAAGACATGGTGCAGTGGACTCGTGCTGAGAAGCGAACTTTCCTTCGGCAGCGAGTGGAGGCCAGGCTTGCAGCTCTTTTGTTGGAAAGCAAGGAGTACTCAGAAGCACTAACTCTCCTCTCTACCTTGATCAAAGAAGTGAGAAGGCTAGATGACAAGCTTCTCCTTGTGGACATAGATCTGTTGGAGAGTAAGCTTCACTTTTCTTTGAGAAATCTCCCTAAAGCTAAGGCTTCACTGACTGCAGCTAGAACAGCAGCCAATTCTATCTATGTGCCTCCAGCTCAACAGGGAACTATTGATTTGCAGAGTGGCATCCTTCATGCAGAAGAAAAAGACTACAAAACTGCTTACAGTTATTTCTTTGAAGCATTTGAAGCCTTCAATGCTCTTGAGGATCCTCGGGCTGTCTTTAGCCTCAAGTATATGTTGTTGTGCAAAATTATGGTGAGCCAGGCTGATGATGTGGCAGGAATTATATCATCCAAAGCAGGCTTGCAGTATGTTGGGCCTGAGCTTGATGCCATGAAGGCAGTGGCTGATGCTCACTCGAAGCGCTCTTTAAAGCTTTTTGAGACTGCTTTGCGGGATTATAAGGCGCAGCTAGAGGATGACCCTATTGTCCACAGGCACCTGTCTTCCCTGTATGACACTCTGTTAGAACAGAATCTCTGCAGGTTGATTGAACCTTTCTCAAGGGTGGAAATAGCTCATATTGCAGAACTGATTGAACTCCCTGTTGACCACGTGGAGAAGAAGCTGTCTCAAATGATTCTGGATAAGAAATTTGCAGGAACTTTGGATCAGGGTGCTGGCTGCCTCATCATTTTTGAGGATCTCAAGACCGACGCCATATACCCTGCTACGCTAGAAACCATATCCAACATGGGTAAGGTTGTTGATAGCCTCTATGTGAGGTCTGCCAAGATAATGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

422

Amino Acids

47.16

Weight (kDa)

6.07

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPN6_N PF18055 16 - 128 5.7e-35 26S proteasome regulatory subunit RPN6 N-terminal domain
PCI PF01399 305 - 387 7.5e-22 PCI domain
RPN6_C_helix PF18503 393 - 418 9.3e-13 26S proteasome subunit RPN6 C-terminal helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 924
AasI GACNNNNNNGTC 1 cut(s) 950
Acc36I ACCTGC 1 cut(s) 996
AccB1I GGYRCC 1 cut(s) 960
AciI CCGC 1 cut(s) 916
AclWI GGATC 4 cut(s) 722, 735, 1140, 1173
AcsI RAATTY 1 cut(s) 1115
AcuI CTGAAG 2 cut(s) 90, 299
AcvI CACGTG 1 cut(s) 1078
AcyI GRCGYC 1 cut(s) 1179
AfaI GTAC 1 cut(s) 428
AfeI AGCGCT 1 cut(s) 887
AfiI CCNNNNNNNGG 1 cut(s) 219
AgsI TTSAA 7 cut(s) 36, 101, 698, 707, 715, 1016, 1061
AjnI CCWGG 2 cut(s) 392, 783
Alw26I GTCTC 2 cut(s) 899, 1098
AlwI GGATC 4 cut(s) 722, 735, 1140, 1173
AlwNI CAGNNNCTG 1 cut(s) 869
Ama87I CYCGRG 1 cut(s) 732
Aor51HI AGCGCT 1 cut(s) 887
AoxI GGCC 5 cut(s) 54, 144, 221, 390, 839
ApeKI GCWGC 6 cut(s) 379, 401, 575, 587, 931, 1146
ApoI RAATTY 1 cut(s) 1115
Asp700I GAANNNNTTC 1 cut(s) 195
AspLEI GCGC 2 cut(s) 888, 931
AspS9I GGNCC 1 cut(s) 839
AsuC2I CCSGG 1 cut(s) 300
AsuHPI GGTGA 2 cut(s) 142, 790
AvaI CYCGRG 1 cut(s) 732
BamHI GGATCC 1 cut(s) 727
BanI GGYRCC 1 cut(s) 960
BauI CACGAG 1 cut(s) 351
BbrPI CACGTG 1 cut(s) 1078
BbsI GAAGAC 1 cut(s) 960
BbvI GCAGC 6 cut(s) 391, 413, 587, 599, 943, 1133
BccI CCATC 2 cut(s) 73, 155
BcgI CGANNNNNNTGC 2 cut(s) 332, 366
BciT130I CCWGG 2 cut(s) 394, 785
BclI TGATCA 1 cut(s) 459
BcnI CCSGG 1 cut(s) 300
BcoDI GTCTC 2 cut(s) 899, 1098
BfaI CTAG 4 cut(s) 479, 579, 935, 1199
BfmI CTRYAG 2 cut(s) 573, 1003
BfoI RGCGCY 1 cut(s) 889
BfuAI ACCTGC 1 cut(s) 996
BglI GCCNNNNNGGC 1 cut(s) 860
BglII AGATCT 3 cut(s) 163, 193, 508
BisI GCNGC 6 cut(s) 380, 402, 576, 588, 932, 1147
BlsI GCNGC 6 cut(s) 381, 403, 577, 589, 933, 1148
BmcAI AGTACT 1 cut(s) 428
Bme1390I CCNGG 3 cut(s) 300, 394, 785
BmeT110I CYCGRG 1 cut(s) 732
BmgT120I GGNCC 1 cut(s) 839
BmiI GGNNCC 2 cut(s) 729, 962
BmrFI CCNGG 3 cut(s) 300, 394, 785
BmrI ACTGGG 1 cut(s) 208
BmsI GCATC 3 cut(s) 654, 841, 862
BmuI ACTGGG 1 cut(s) 208
BpiI GAAGAC 1 cut(s) 960
BpmI CTGGAG 1 cut(s) 594
Bpu10I CCTNAGC 2 cut(s) 558, 843
BpuEI CTTGAG 4 cut(s) 734, 743, 1009, 1154
BpuMI CCSGG 1 cut(s) 300
BsaAI YACGTR 1 cut(s) 1078
BsaHI GRCGYC 1 cut(s) 1179
BsaJI CCNNGG 1 cut(s) 731
BsaXI ACNNNNNCTCC 2 cut(s) 429, 459
Bsc4I CCNNNNNNNGG 1 cut(s) 219
Bse1I ACTGG 1 cut(s) 214
BseBI CCWGG 2 cut(s) 394, 785
BseDI CCNNGG 1 cut(s) 731
BseGI GGATG 3 cut(s) 645, 812, 946
BseLI CCNNNNNNNGG 1 cut(s) 219
BseMII CTCAG 3 cut(s) 348, 444, 834
BseNI ACTGG 1 cut(s) 214
BseRI GAGGAG 1 cut(s) 437
BseXI GCAGC 6 cut(s) 391, 413, 587, 599, 943, 1133
BseYI CCCAGC 1 cut(s) 19
BsgI GTGCAG 1 cut(s) 362
BshFI GGCC 5 cut(s) 56, 146, 223, 392, 841
BshNI GGYRCC 1 cut(s) 960
BsiHKCI CYCGRG 1 cut(s) 732
BsiSI CCGG 1 cut(s) 300
BslI CCNNNNNNNGG 1 cut(s) 219
BsmAI GTCTC 2 cut(s) 899, 1098
BsnI GGCC 5 cut(s) 56, 146, 223, 392, 841
BsoBI CYCGRG 1 cut(s) 732
Bsp143I GATC 7 cut(s) 163, 193, 459, 508, 727, 1132, 1165
BspACI CCGC 1 cut(s) 916
BspANI GGCC 5 cut(s) 56, 146, 223, 392, 841
BspCNI CTCAG 3 cut(s) 349, 443, 835
BspLI GGNNCC 2 cut(s) 729, 962
BspMAI CTGCAG 2 cut(s) 577, 1007
BspMI ACCTGC 1 cut(s) 996
BspPI GGATC 4 cut(s) 722, 735, 1140, 1173
BspT107I GGYRCC 1 cut(s) 960
BsrI ACTGG 1 cut(s) 214
BssECI CCNNGG 1 cut(s) 731
BssMI GATC 7 cut(s) 163, 193, 459, 508, 727, 1132, 1165
BssNI GRCGYC 1 cut(s) 1179
BssSI CACGAG 1 cut(s) 351
Bst2BI CACGAG 1 cut(s) 351
Bst2UI CCWGG 2 cut(s) 394, 785
Bst4CI ACNGT 2 cut(s) 286, 686
BstACI GRCGYC 1 cut(s) 1179
BstAPI GCANNNNNTGC 1 cut(s) 254
BstBAI YACGTR 1 cut(s) 1078
BstC8I GCNNGC 4 cut(s) 399, 823, 827, 1144
BstDEI CTNAG 5 cut(s) 170, 357, 430, 558, 843
BstF5I GGATG 3 cut(s) 645, 812, 946
BstH2I RGCGCY 1 cut(s) 889
BstHHI GCGC 2 cut(s) 888, 931
BstKTI GATC 7 cut(s) 166, 196, 462, 511, 730, 1135, 1168
BstMAI GTCTC 2 cut(s) 899, 1098
BstMBI GATC 7 cut(s) 163, 193, 459, 508, 727, 1132, 1165
BstMWI GCNNNNNNNGC 8 cut(s) 53, 254, 398, 584, 707, 716, 860, 1046
BstNI CCWGG 2 cut(s) 394, 785
BstSCI CCNGG 3 cut(s) 298, 392, 783
BstSFI CTRYAG 2 cut(s) 573, 1003
BstV1I GCAGC 6 cut(s) 391, 413, 587, 599, 943, 1133
BstV2I GAAGAC 1 cut(s) 960
BstX2I RGATCY 5 cut(s) 163, 193, 508, 727, 1165
BstXI CCANNNNNNTGG 1 cut(s) 1261
BstYI RGATCY 5 cut(s) 163, 193, 508, 727, 1165
BsuRI GGCC 5 cut(s) 56, 146, 223, 392, 841
BtsCI GGATG 3 cut(s) 645, 812, 946
BtsI GCAGTG 2 cut(s) 350, 870
BtsIMutI CAGTG 3 cut(s) 350, 566, 870
BveI ACCTGC 1 cut(s) 996
Cac8I GCNNGC 4 cut(s) 399, 823, 827, 1144
CaiI CAGNNNCTG 1 cut(s) 869
CfoI GCGC 2 cut(s) 888, 931
Cfr13I GGNCC 1 cut(s) 839
CseI GACGC 1 cut(s) 1187
Csp6I GTAC 1 cut(s) 427
CviAII CATG 5 cut(s) 337, 653, 856, 1216, 1266
CviQI GTAC 1 cut(s) 427
DdeI CTNAG 5 cut(s) 170, 357, 430, 558, 843
DpnI GATC 7 cut(s) 165, 195, 461, 510, 729, 1134, 1167
DpnII GATC 7 cut(s) 163, 193, 459, 508, 727, 1132, 1165
DraI TTTAAA 1 cut(s) 894
DrdI GACNNNNNNGTC 1 cut(s) 950
DseDI GACNNNNNNGTC 1 cut(s) 950
Eco147I AGGCCT 1 cut(s) 223
Eco47III AGCGCT 1 cut(s) 887
Eco57I CTGAAG 2 cut(s) 90, 299
Eco72I CACGTG 1 cut(s) 1078
Eco88I CYCGRG 1 cut(s) 732
EcoRII CCWGG 2 cut(s) 392, 783
FaeI CATG 5 cut(s) 340, 656, 859, 1219, 1269
FalI AAGNNNNNCTT 2 cut(s) 515, 547
FatI CATG 5 cut(s) 336, 652, 855, 1215, 1265
FauI CCCGC 1 cut(s) 909
FbaI TGATCA 1 cut(s) 459
Fnu4HI GCNGC 6 cut(s) 380, 402, 576, 588, 932, 1147
FokI GGATG 3 cut(s) 632, 799, 953
Fsp4HI GCNGC 6 cut(s) 380, 402, 576, 588, 932, 1147
FspBI CTAG 4 cut(s) 479, 579, 935, 1199
GlaI GCGC 2 cut(s) 887, 930
GluI GCNGC 6 cut(s) 380, 402, 576, 588, 932, 1147
GsaI CCCAGC 1 cut(s) 23
GsuI CTGGAG 1 cut(s) 594
HaeII RGCGCY 1 cut(s) 889
HaeIII GGCC 5 cut(s) 56, 146, 223, 392, 841
HapII CCGG 1 cut(s) 300
HgaI GACGC 1 cut(s) 1187
HhaI GCGC 2 cut(s) 888, 931
Hin1I GRCGYC 1 cut(s) 1179
Hin1II CATG 5 cut(s) 340, 656, 859, 1219, 1269
Hin6I GCGC 2 cut(s) 886, 929
HinP1I GCGC 2 cut(s) 886, 929
HincII GTYRAC 2 cut(s) 6, 1072
HindII GTYRAC 2 cut(s) 6, 1072
HindIII AAGCTT 3 cut(s) 488, 524, 896
HinfI GANTC 6 cut(s) 32, 181, 238, 349, 997, 1102
HpaII CCGG 1 cut(s) 300
HphI GGTGA 2 cut(s) 142, 790
Hpy166II GTNNAC 5 cut(s) 6, 348, 502, 955, 1072
Hpy188I TCNGA 6 cut(s) 70, 121, 163, 310, 318, 433
Hpy188III TCNNGA 4 cut(s) 98, 722, 1106, 1171
Hpy8I GTNNAC 5 cut(s) 6, 348, 502, 955, 1072
Hpy99I CGWCG 1 cut(s) 1181
HpyAV CCTTC 6 cut(s) 215, 383, 468, 659, 721, 853
HpyCH4III ACNGT 2 cut(s) 286, 686
HpyCH4IV ACGT 1 cut(s) 1077
HpyF10VI GCNNNNNNNGC 8 cut(s) 53, 254, 398, 584, 707, 716, 860, 1046
HpyF3I CTNAG 5 cut(s) 170, 357, 430, 558, 843
HpySE526I ACGT 1 cut(s) 1077
Hsp92I GRCGYC 1 cut(s) 1179
Hsp92II CATG 5 cut(s) 340, 656, 859, 1219, 1269
HspAI GCGC 2 cut(s) 886, 929
Ksp22I TGATCA 1 cut(s) 459
Kzo9I GATC 7 cut(s) 163, 193, 459, 508, 727, 1132, 1165
LmnI GCTCC 1 cut(s) 67
Lsp1109I GCAGC 6 cut(s) 391, 413, 587, 599, 943, 1133
LweI GCATC 3 cut(s) 654, 841, 862
MaeI CTAG 4 cut(s) 479, 579, 935, 1199
MaeII ACGT 1 cut(s) 1077
MaeIII GTNAC 1 cut(s) 200
MalI GATC 7 cut(s) 165, 195, 461, 510, 729, 1134, 1167
MboI GATC 7 cut(s) 163, 193, 459, 508, 727, 1132, 1165
MboII GAAGA 7 cut(s) 108, 158, 188, 203, 671, 960, 1096
MfeI CAATTG 1 cut(s) 36
MflI RGATCY 5 cut(s) 163, 193, 508, 727, 1165
MluCI AATT 5 cut(s) 36, 592, 771, 804, 1115
MlyI GAGTC 1 cut(s) 343
MmeI TCCRAC 3 cut(s) 393, 495, 1236
MroXI GAANNNNTTC 1 cut(s) 195
MseI TTAA 1 cut(s) 893
MspI CCGG 1 cut(s) 300
MspR9I CCNGG 3 cut(s) 300, 394, 785
MunI CAATTG 1 cut(s) 36
MvaI CCWGG 2 cut(s) 394, 785
MwoI GCNNNNNNNGC 8 cut(s) 53, 254, 398, 584, 707, 716, 860, 1046
NciI CCSGG 1 cut(s) 300
NdeII GATC 7 cut(s) 163, 193, 459, 508, 727, 1132, 1165
NlaIII CATG 5 cut(s) 340, 656, 859, 1219, 1269
NlaIV GGNNCC 2 cut(s) 729, 962
PceI AGGCCT 1 cut(s) 223
PdmI GAANNNNTTC 1 cut(s) 195
PfeI GAWTC 5 cut(s) 32, 181, 238, 997, 1102
PkrI GCNGC 6 cut(s) 381, 403, 577, 589, 933, 1148
PleI GAGTC 1 cut(s) 343
PmaCI CACGTG 1 cut(s) 1078
PmlI CACGTG 1 cut(s) 1078
PpsI GAGTC 1 cut(s) 343
Ppu21I YACGTR 1 cut(s) 1078
PsiI TTATAA 1 cut(s) 924
Psp6I CCWGG 2 cut(s) 392, 783
PspCI CACGTG 1 cut(s) 1078
PspFI CCCAGC 1 cut(s) 19
PspGI CCWGG 2 cut(s) 392, 783
PspN4I GGNNCC 2 cut(s) 729, 962
PspPI GGNCC 1 cut(s) 839
PstI CTGCAG 2 cut(s) 577, 1007
PstNI CAGNNNCTG 1 cut(s) 869
PsuI RGATCY 5 cut(s) 163, 193, 508, 727, 1165
RsaI GTAC 1 cut(s) 428
RsaNI GTAC 1 cut(s) 427
SaqAI TTAA 1 cut(s) 893
SatI GCNGC 6 cut(s) 380, 402, 576, 588, 932, 1147
Sau3AI GATC 7 cut(s) 163, 193, 459, 508, 727, 1132, 1165
Sau96I GGNCC 1 cut(s) 839
ScaI AGTACT 1 cut(s) 428
SchI GAGTC 1 cut(s) 343
ScrFI CCNGG 3 cut(s) 300, 394, 785
SfaNI GCATC 3 cut(s) 654, 841, 862
SfcI CTRYAG 2 cut(s) 573, 1003
SmlI CTYRAG 4 cut(s) 722, 749, 1024, 1169
SmoI CTYRAG 4 cut(s) 722, 749, 1024, 1169
Sse9I AATT 5 cut(s) 36, 592, 771, 804, 1115
SseBI AGGCCT 1 cut(s) 223
SsiI CCGC 1 cut(s) 916
SspMI CTAG 4 cut(s) 479, 579, 935, 1199
StuI AGGCCT 1 cut(s) 223
StyD4I CCNGG 3 cut(s) 298, 392, 783
TaaI ACNGT 2 cut(s) 286, 686
TaiI ACGT 1 cut(s) 1080
TaqI TCGA 1 cut(s) 881
TaqII GACCGA 1 cut(s) 1190
TasI AATT 5 cut(s) 36, 592, 771, 804, 1115
TatI WGTACW 1 cut(s) 426
TfiI GAWTC 5 cut(s) 32, 181, 238, 997, 1102
Tru1I TTAA 1 cut(s) 893
Tru9I TTAA 1 cut(s) 893
TscAI CASTG 3 cut(s) 350, 573, 870
TseI GCWGC 6 cut(s) 379, 401, 575, 587, 931, 1146
TspDTI ATGAA 2 cut(s) 641, 872
TspGWI ACGGA 1 cut(s) 44
TspRI CASTG 3 cut(s) 350, 573, 870
XapI RAATTY 1 cut(s) 1115
XcmI CCANNNNNNNNNTGG 2 cut(s) 862, 1213
XmnI GAANNNNTTC 1 cut(s) 195
XspI CTAG 4 cut(s) 479, 579, 935, 1199
ZrmI AGTACT 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.