Rh5DG017000

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000724
Physical Location & Seq
Forward (+)
1313274 .. 1316391
3118 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG017000.1

Sequence Viewer

Length: 2178 bp
ATGGCAGAATATAGCAGCATAAATCCCAAACACCTATATCAAAGCCTAAAGAATGGTGACAAAGCCAAAGTGATGGAATATTGTTATGATAAAACTAGATCTGATGTTGAAAGGTTGAAGCTCATTCCAACAGTGCACGATGATACAATCCTCCATTTAGCAATTAACATGGGGCAGCAAGACATAGCAAAAGAAATCATCAAAAGATGTTCCGGGGGTTCTAATGATAAGCTACTTATGAAGAAGAATGCCCTCGGAAACACAGTTCTGCACGAGGCTGCAGCAACAAGTATGACAAGCCTTGTGGAGGAGTTGTTAAGAAAGGCATCAGACTTGTTGTCTATTTCGAACAACAAAAATGAAATGCCTCTCTATACGGCAGCTTACTATGGCCAAACCGAAATGTTCAAGTTGTTGGCGGACGAAGTAGACAAAAACCAGTCTGGCAATCTTGATCTTGATCTTCATTTGCGACCTAGGGGTCGTAAGACGAACCCCAATAAAGTGCGGACTACCATTCTTCATGCTGCAATACATGCCGAGTTCTTTGAGCTTGCACTTCTGATAGCAAAAAGGTTTACGCAACTAGTTAAGAAAAAGGATGAAAATCGGCTGACAGGCCTTCAGCTGCTTTCATGTAATCCTTCAGCTTTCAGGAGTGGAACCAAATATGGACCGATCAAGAGGTTCATTTATTCCTGTGCTCCATATGAGAATGTGATAATGGATAAAAATGACAGCCACGAACACGAAGAGGCTGATGTATACATTTTCAGCGATCTAGGTGACGAGGAAAATACAGCTTGCTTCAAGATGCCTCAGAGTCAAACATGTACATATATGAATAACCGCTGCACCATTGCTTTACGGCAAGCCATCTCTTCTGTTAATAGAGCGGTTTGGAAAATTCTTCAAGCTTGGGGCACAATGAAGAGGATCTATGGGGACAAGAAGAGGCATGAATCAGCCTTCAAACTAGCCAAGTTACTAATTGCAGCAGACTTTTCATGGGACAGCATAGGAGTACAAGATGAAATCGCTAGCTCAAAGGTCACCGCTGCTACAAATGATGCTCCCTCTTTGGATCGCAAAGCTGATATTGATTGGATAGAAGCCCTTTTAGGTGCAGAAGCCTTAAGTGGCAGCGGTGCTGTTAGTGGCGGCCCAGAAATAGCAGCTTCATCCATGTCAGAACTTCATTCACCTCCAACGCCATTGCTCCTTGCAACAAGCCACGGAATACTAGAAATTGTGGATGAGATACTTAAAGTCTACCCTCTGGCAGTTGAGCATATAAGTGAAAAGGGGCAAAACTTGCTGCATGTTGCTGTTCTGCACCGCCAGTTGGACGTCTTTCAACGAGTCATTAGGATGGAATCACCCAAGTCAAGGTTGGTTCGACGTATCGACAACAATGGATTTACAATCTTGCACCATGTTGGAAACATGGCCTTTTACAAAGGAAGCACACAGCCTGGTCCTGCACTCCAGTTGCAAGAAGAGTTAGAATGGTTTGAGGTACTTACGGTAATTCCACCCCATTATGCGATGCAACTTGCCTGGAACGAAAAGGAACAGAAAAACGAGACCCCCAAACAGTACTTTCGTCGCACACATGGCGAGCTTCTCAAGGAGGCCCAAGATTGGATAAAGCGGACCGCCGAGTCGTGCTCGACGGTGGCAGGACTAATGGCCACCGTCGCTTTCGCCGCCGCCTTCACCGTCCCTGGAGGCAACGACGAGAAAAGCGGCACTCCACTCCTTCTTCACAGCCCTTTCTTCTTAGTCTTCATCATCACCGACGCTTTCTCCCTAGCCAGCTCGCTCACGTCACTCGTCATGTTCCTCTCGATTCTGACGTCACCGTACGAGATCGACGACTTCCGCTACTCTCTCCCTCGGAAGCTTATCCTCGGCTTCACCTTCCTCTTCTTCTCGGTGGCGGTGACCATGCTGGCCTTTGCTTCCACACTGATGCTGACAATTTCGACAAAGAAGAGCCTGACAACAACTTTTATATACTGCGTTGCGTTTCTTCCGGTCACTATGTTCGCGCTGTTGCAGTTCCCTTTGTACGTGGCCTTCAAAACGACTCTCCTGTACTCTGTACAGGCCATCCAAAGCGTTCTTCCGTGGCATCTCATCTCTGTTGGTTTTTCAAAACTCAAAAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

725

Amino Acids

81.16

Weight (kDa)

7.94

Isoelectric Point (pI)

40.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 51 - 147 1.1e-09 Ankyrin repeats (3 copies)
PGG PF13962 548 - 660 9.8e-25 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 480, 1663
AatII GACGTC 2 cut(s) 1353, 1862
AccBSI CCGCTC 1 cut(s) 896
AccI GTMKAC 3 cut(s) 429, 765, 1272
AccII CGCG 1 cut(s) 2054
AclWI GGATC 2 cut(s) 944, 1092
AcoI YGGCCR 2 cut(s) 391, 1692
AcsI RAATTY 1 cut(s) 906
AcuI CTGAAG 2 cut(s) 608, 630
AcyI GRCGYC 2 cut(s) 1350, 1859
AfaI GTAC 8 cut(s) 835, 1026, 1521, 1601, 1868, 2075, 2102, 2109
AfiI CCNNNNNNNGG 4 cut(s) 307, 1345, 1389, 1644
AflII CTTAAG 1 cut(s) 1135
AflIII ACRYGT 1 cut(s) 830
AgsI TTSAA 9 cut(s) 110, 118, 409, 811, 914, 973, 1358, 2086, 2160
AhdI GACNNNNNGTC 1 cut(s) 337
AhlI ACTAGT 1 cut(s) 586
AjiI CACGTC 1 cut(s) 1830
AjnI CCWGG 3 cut(s) 1474, 1559, 1726
AjuI GAANNNNNNNTTGG 2 cut(s) 2112, 2144
AloI GAACNNNNNNTCC 2 cut(s) 249, 281
Alw21I GWGCWC 3 cut(s) 138, 706, 1673
Alw26I GTCTC 1 cut(s) 1580
Alw44I GTGCAC 1 cut(s) 134
AlwI GGATC 2 cut(s) 944, 1092
AoxI GGCC 9 cut(s) 391, 619, 1162, 1449, 1635, 1692, 1956, 2079, 2112
ApaLI GTGCAC 1 cut(s) 134
ApoI RAATTY 1 cut(s) 906
AspA2I CCTAGG 1 cut(s) 476
AspLEI GCGC 1 cut(s) 2056
AspS9I GGNCC 5 cut(s) 674, 1163, 1478, 1636, 1656
AsuC2I CCSGG 1 cut(s) 214
AsuII TTCGAA 1 cut(s) 347
AsuNHI GCTAGC 1 cut(s) 1040
AvaII GGWCC 3 cut(s) 674, 1478, 1656
AvrII CCTAGG 1 cut(s) 476
BaeGI GKGCMC 2 cut(s) 138, 926
BalI TGGCCA 2 cut(s) 393, 1694
BarI GAAGNNNNNNTAC 2 cut(s) 2066, 2098
BauI CACGAG 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 1780
Bbv12I GWGCWC 3 cut(s) 138, 706, 1673
BccI CCATC 4 cut(s) 67, 884, 1366, 2123
BceAI ACGGC 2 cut(s) 393, 884
BciT130I CCWGG 3 cut(s) 1476, 1561, 1728
BcnI CCSGG 1 cut(s) 214
BcoDI GTCTC 1 cut(s) 1580
BcuI ACTAGT 1 cut(s) 586
BfaI CTAG 8 cut(s) 96, 477, 587, 782, 977, 1041, 1244, 1814
BfmI CTRYAG 1 cut(s) 279
BfrI CTTAAG 1 cut(s) 1135
BglII AGATCT 1 cut(s) 98
BlnI CCTAGG 1 cut(s) 476
BmcAI AGTACT 1 cut(s) 1601
Bme1390I CCNGG 4 cut(s) 214, 1476, 1561, 1728
Bme18I GGWCC 3 cut(s) 674, 1478, 1656
BmeRI GACNNNNNGTC 1 cut(s) 337
BmgBI CACGTC 1 cut(s) 1830
BmgT120I GGNCC 5 cut(s) 674, 1163, 1478, 1636, 1656
BmiI GGNNCC 1 cut(s) 664
BmrFI CCNGG 4 cut(s) 214, 1476, 1561, 1728
BmsI GCATC 6 cut(s) 335, 804, 1060, 1539, 1965, 2146
BmtI GCTAGC 1 cut(s) 1044
BpiI GAAGAC 1 cut(s) 1780
BplI GAGNNNNNCTC 2 cut(s) 1655, 1687
BpmI CTGGAG 2 cut(s) 1472, 1749
Bpu14I TTCGAA 1 cut(s) 347
BpuEI CTTGAG 1 cut(s) 1613
BpuMI CCSGG 1 cut(s) 214
BsaAI YACGTR 1 cut(s) 2077
BsaBI GATNNNNATC 2 cut(s) 459, 606
BsaHI GRCGYC 2 cut(s) 1350, 1859
BsaI GGTCTC 1 cut(s) 1580
BsaJI CCNNGG 8 cut(s) 213, 253, 476, 1234, 1726, 1898, 1912, 2132
BsaWI WCCGGW 1 cut(s) 2038
BsaXI ACNNNNNCTCC 4 cut(s) 299, 329, 1793, 1823
Bsc4I CCNNNNNNNGG 4 cut(s) 307, 1345, 1389, 1644
Bse1I ACTGG 3 cut(s) 439, 1342, 1489
Bse3DI GCAATG 2 cut(s) 858, 1214
Bse8I GATNNNNATC 2 cut(s) 459, 606
BseBI CCWGG 3 cut(s) 1476, 1561, 1728
BseDI CCNNGG 8 cut(s) 213, 253, 476, 1234, 1726, 1898, 1912, 2132
BseGI GGATG 5 cut(s) 607, 1181, 1261, 1377, 2115
BseJI GATNNNNATC 2 cut(s) 459, 606
BseLI CCNNNNNNNGG 4 cut(s) 307, 1345, 1389, 1644
BseMI GCAATG 2 cut(s) 858, 1214
BseMII CTCAG 1 cut(s) 833
BseNI ACTGG 3 cut(s) 439, 1342, 1489
BseRI GAGGAG 1 cut(s) 323
BseSI GKGCMC 2 cut(s) 138, 926
BsgI GTGCAG 5 cut(s) 254, 838, 1146, 1319, 1467
Bsh1236I CGCG 1 cut(s) 2054
BshFI GGCC 9 cut(s) 393, 621, 1164, 1451, 1637, 1694, 1958, 2081, 2114
BsiHKAI GWGCWC 3 cut(s) 138, 706, 1673
BsiSI CCGG 2 cut(s) 213, 2039
BsiWI CGTACG 1 cut(s) 1866
BslFI GGGAC 3 cut(s) 959, 1025, 1709
BslI CCNNNNNNNGG 4 cut(s) 307, 1345, 1389, 1644
BsmAI GTCTC 1 cut(s) 1580
BsmFI GGGAC 3 cut(s) 959, 1025, 1709
BsmI GAATGC 1 cut(s) 253
BsnI GGCC 9 cut(s) 393, 621, 1164, 1451, 1637, 1694, 1958, 2081, 2114
Bso31I GGTCTC 1 cut(s) 1580
Bsp119I TTCGAA 1 cut(s) 347
Bsp1286I GDGCHC 4 cut(s) 138, 706, 926, 1673
Bsp1407I TGTACA 2 cut(s) 833, 2107
Bsp143I GATC 8 cut(s) 98, 454, 460, 678, 778, 936, 1084, 1872
BspANI GGCC 9 cut(s) 393, 621, 1164, 1451, 1637, 1694, 1958, 2081, 2114
BspCNI CTCAG 1 cut(s) 832
BspFNI CGCG 1 cut(s) 2054
BspLI GGNNCC 1 cut(s) 664
BspMAI CTGCAG 1 cut(s) 283
BspOI GCTAGC 1 cut(s) 1044
BspPI GGATC 2 cut(s) 944, 1092
BspQI GCTCTTC 1 cut(s) 1991
BspT104I TTCGAA 1 cut(s) 347
BspTI CTTAAG 1 cut(s) 1135
BspTNI GGTCTC 1 cut(s) 1580
BsrBI CCGCTC 1 cut(s) 896
BsrDI GCAATG 2 cut(s) 858, 1214
BsrGI TGTACA 2 cut(s) 833, 2107
BsrI ACTGG 3 cut(s) 439, 1342, 1489
BssECI CCNNGG 8 cut(s) 213, 253, 476, 1234, 1726, 1898, 1912, 2132
BssMI GATC 8 cut(s) 98, 454, 460, 678, 778, 936, 1084, 1872
BssNAI GTATAC 1 cut(s) 766
BssNI GRCGYC 2 cut(s) 1350, 1859
BssSI CACGAG 1 cut(s) 272
BssT1I CCWWGG 1 cut(s) 476
Bst1107I GTATAC 1 cut(s) 766
Bst2BI CACGAG 1 cut(s) 272
Bst2UI CCWGG 3 cut(s) 1476, 1561, 1728
Bst4CI ACNGT 8 cut(s) 133, 265, 1528, 1599, 1678, 1699, 1723, 1866
Bst6I CTCTTC 7 cut(s) 747, 886, 926, 947, 1494, 1934, 1991
BstACI GRCGYC 2 cut(s) 1350, 1859
BstAFI CTTAAG 1 cut(s) 1135
BstAPI GCANNNNNTGC 2 cut(s) 536, 1315
BstAUI TGTACA 2 cut(s) 833, 2107
BstBAI YACGTR 1 cut(s) 2077
BstBI TTCGAA 1 cut(s) 347
BstC8I GCNNGC 8 cut(s) 555, 805, 873, 1042, 1622, 1819, 1823, 1956
BstDEI CTNAG 2 cut(s) 819, 1783
BstDSI CCRYGG 2 cut(s) 1234, 2132
BstEII GGTNACC 2 cut(s) 1051, 1945
BstENI CCTNNNNNAGG 1 cut(s) 305
BstF5I GGATG 5 cut(s) 607, 1181, 1261, 1377, 2115
BstFNI CGCG 1 cut(s) 2054
BstHHI GCGC 1 cut(s) 2056
BstKTI GATC 8 cut(s) 101, 457, 463, 681, 781, 939, 1087, 1875
BstMAI GTCTC 1 cut(s) 1580
BstMBI GATC 8 cut(s) 98, 454, 460, 678, 778, 936, 1084, 1872
BstMWI GCNNNNNNNGC 5 cut(s) 536, 1315, 1617, 1700, 1709
BstNI CCWGG 3 cut(s) 1476, 1561, 1728
BstNSI RCATGY 3 cut(s) 539, 834, 1325
BstPI GGTNACC 2 cut(s) 1051, 1945
BstSCI CCNGG 4 cut(s) 212, 1474, 1559, 1726
BstSFI CTRYAG 1 cut(s) 279
BstSLI GKGCMC 2 cut(s) 138, 926
BstUI CGCG 1 cut(s) 2054
BstV2I GAAGAC 1 cut(s) 1780
BstX2I RGATCY 2 cut(s) 98, 936
BstXI CCANNNNNNTGG 1 cut(s) 73
BstYI RGATCY 2 cut(s) 98, 936
BstZ17I GTATAC 1 cut(s) 766
BsuRI GGCC 9 cut(s) 393, 621, 1164, 1451, 1637, 1694, 1958, 2081, 2114
BtgI CCRYGG 2 cut(s) 1234, 2132
BtgZI GCGATG 1 cut(s) 1562
BtrI CACGTC 1 cut(s) 1830
BtsCI GGATG 5 cut(s) 607, 1181, 1261, 1377, 2115
BtsIMutI CAGTG 2 cut(s) 138, 1970
Cac8I GCNNGC 8 cut(s) 555, 805, 873, 1042, 1622, 1819, 1823, 1956
CfoI GCGC 1 cut(s) 2056
Cfr13I GGNCC 5 cut(s) 674, 1163, 1478, 1636, 1656
CpoI CGGWCCG 1 cut(s) 1656
CseI GACGC 1 cut(s) 1811
Csp6I GTAC 8 cut(s) 834, 1025, 1520, 1600, 1867, 2074, 2101, 2108
CspCI CAANNNNNGTGG 2 cut(s) 285, 320
CspI CGGWCCG 1 cut(s) 1656
CviQI GTAC 8 cut(s) 834, 1025, 1520, 1600, 1867, 2074, 2101, 2108
DdeI CTNAG 2 cut(s) 819, 1783
DpnI GATC 8 cut(s) 100, 456, 462, 680, 780, 938, 1086, 1874
DpnII GATC 8 cut(s) 98, 454, 460, 678, 778, 936, 1084, 1872
DrdI GACNNNNNNGTC 2 cut(s) 480, 1663
DriI GACNNNNNGTC 1 cut(s) 337
DseDI GACNNNNNNGTC 2 cut(s) 480, 1663
EaeI YGGCCR 2 cut(s) 391, 1692
Eam1104I CTCTTC 7 cut(s) 747, 886, 926, 947, 1494, 1934, 1991
Eam1105I GACNNNNNGTC 1 cut(s) 337
EarI CTCTTC 7 cut(s) 747, 886, 926, 947, 1494, 1934, 1991
EciI GGCGGA 1 cut(s) 434
Eco130I CCWWGG 1 cut(s) 476
Eco147I AGGCCT 1 cut(s) 621
Eco31I GGTCTC 1 cut(s) 1580
Eco47I GGWCC 3 cut(s) 674, 1478, 1656
Eco57I CTGAAG 2 cut(s) 608, 630
Eco91I GGTNACC 2 cut(s) 1051, 1945
EcoNI CCTNNNNNAGG 1 cut(s) 305
EcoO65I GGTNACC 2 cut(s) 1051, 1945
EcoRII CCWGG 3 cut(s) 1474, 1559, 1726
EcoT14I CCWWGG 1 cut(s) 476
ErhI CCWWGG 1 cut(s) 476
FaqI GGGAC 3 cut(s) 959, 1025, 1709
FauNDI CATATG 1 cut(s) 709
FblI GTMKAC 3 cut(s) 429, 765, 1272
FokI GGATG 5 cut(s) 614, 1168, 1268, 1384, 2102
FspBI CTAG 8 cut(s) 96, 477, 587, 782, 977, 1041, 1244, 1814
GlaI GCGC 1 cut(s) 2055
GsuI CTGGAG 2 cut(s) 1472, 1749
HaeIII GGCC 9 cut(s) 393, 621, 1164, 1451, 1637, 1694, 1958, 2081, 2114
HapII CCGG 2 cut(s) 213, 2039
HgaI GACGC 1 cut(s) 1811
HhaI GCGC 1 cut(s) 2056
Hin1I GRCGYC 2 cut(s) 1350, 1859
Hin6I GCGC 1 cut(s) 2054
HinP1I GCGC 1 cut(s) 2054
HindIII AAGCTT 2 cut(s) 915, 1904
HinfI GANTC 7 cut(s) 823, 962, 1362, 1376, 1664, 1852, 2092
HpaII CCGG 2 cut(s) 213, 2039
Hpy166II GTNNAC 5 cut(s) 136, 430, 579, 766, 1273
Hpy188I TCNGA 8 cut(s) 103, 257, 331, 564, 822, 1192, 1857, 1902
Hpy188III TCNNGA 6 cut(s) 452, 458, 655, 682, 811, 1849
Hpy8I GTNNAC 5 cut(s) 136, 430, 579, 766, 1273
Hpy99I CGWCG 7 cut(s) 1404, 1611, 1678, 1703, 1742, 1805, 1880
HpyAV CCTTC 7 cut(s) 632, 654, 979, 1726, 1772, 1933, 2092
HpyCH4III ACNGT 8 cut(s) 133, 265, 1528, 1599, 1678, 1699, 1723, 1866
HpyCH4IV ACGT 5 cut(s) 1350, 1402, 1829, 1859, 2076
HpyF10VI GCNNNNNNNGC 5 cut(s) 536, 1315, 1617, 1700, 1709
HpyF3I CTNAG 2 cut(s) 819, 1783
HpySE526I ACGT 5 cut(s) 1350, 1402, 1829, 1859, 2076
Hsp92I GRCGYC 2 cut(s) 1350, 1859
HspAI GCGC 1 cut(s) 2054
Kzo9I GATC 8 cut(s) 98, 454, 460, 678, 778, 936, 1084, 1872
LguI GCTCTTC 1 cut(s) 1991
LmnI GCTCC 3 cut(s) 709, 1078, 1224
LweI GCATC 6 cut(s) 335, 804, 1060, 1539, 1965, 2146
MaeI CTAG 8 cut(s) 96, 477, 587, 782, 977, 1041, 1244, 1814
MaeII ACGT 5 cut(s) 1350, 1402, 1829, 1859, 2076
MaeIII GTNAC 8 cut(s) 56, 785, 984, 1051, 1830, 1860, 1945, 2041
MalI GATC 8 cut(s) 100, 456, 462, 680, 780, 938, 1086, 1874
MbiI CCGCTC 1 cut(s) 896
MboI GATC 8 cut(s) 98, 454, 460, 678, 778, 936, 1084, 1872
MflI RGATCY 2 cut(s) 98, 936
MhlI GDGCHC 4 cut(s) 138, 706, 926, 1673
MlsI TGGCCA 2 cut(s) 393, 1694
MluCI AATT 7 cut(s) 162, 906, 990, 1248, 1530, 1983, 2173
MluNI TGGCCA 2 cut(s) 393, 1694
MlyI GAGTC 4 cut(s) 832, 1371, 1673, 2086
MmeI TCCRAC 4 cut(s) 152, 1232, 1326, 1420
Mox20I TGGCCA 2 cut(s) 393, 1694
MscI TGGCCA 2 cut(s) 393, 1694
MseI TTAA 7 cut(s) 165, 317, 591, 888, 1136, 1266, 2176
MslI CAYNNNNRTG 3 cut(s) 1296, 1370, 1973
Msp20I TGGCCA 2 cut(s) 393, 1694
MspA1I CMGCKG 4 cut(s) 628, 852, 1058, 1146
MspCI CTTAAG 1 cut(s) 1135
MspI CCGG 2 cut(s) 213, 2039
MspR9I CCNGG 4 cut(s) 214, 1476, 1561, 1728
Mva1269I GAATGC 1 cut(s) 253
MvaI CCWGG 3 cut(s) 1476, 1561, 1728
MvnI CGCG 1 cut(s) 2054
MwoI GCNNNNNNNGC 5 cut(s) 536, 1315, 1617, 1700, 1709
NciI CCSGG 1 cut(s) 214
NdeI CATATG 1 cut(s) 709
NdeII GATC 8 cut(s) 98, 454, 460, 678, 778, 936, 1084, 1872
NheI GCTAGC 1 cut(s) 1040
NlaIV GGNNCC 1 cut(s) 664
NmeAIII GCCGAG 3 cut(s) 565, 1687, 1893
NmuCI GTSAC 7 cut(s) 56, 785, 1051, 1830, 1860, 1945, 2041
NspI RCATGY 3 cut(s) 539, 834, 1325
NspV TTCGAA 1 cut(s) 347
PceI AGGCCT 1 cut(s) 621
PciI ACATGT 1 cut(s) 830
PciSI GCTCTTC 1 cut(s) 1991
PcsI WCGNNNNNNNCGW 2 cut(s) 1856, 1875
PctI GAATGC 1 cut(s) 253
PfeI GAWTC 3 cut(s) 962, 1376, 1852
Pfl23II CGTACG 1 cut(s) 1866
PleI GAGTC 4 cut(s) 831, 1370, 1672, 2086
PpsI GAGTC 4 cut(s) 831, 1370, 1672, 2086
Ppu21I YACGTR 1 cut(s) 2077
PscI ACATGT 1 cut(s) 830
Psp6I CCWGG 3 cut(s) 1474, 1559, 1726
PspEI GGTNACC 2 cut(s) 1051, 1945
PspGI CCWGG 3 cut(s) 1474, 1559, 1726
PspLI CGTACG 1 cut(s) 1866
PspN4I GGNNCC 1 cut(s) 664
PspPI GGNCC 5 cut(s) 674, 1163, 1478, 1636, 1656
PstI CTGCAG 1 cut(s) 283
PsuI RGATCY 2 cut(s) 98, 936
PvuII CAGCTG 1 cut(s) 628
RsaI GTAC 8 cut(s) 835, 1026, 1521, 1601, 1868, 2075, 2102, 2109
RsaNI GTAC 8 cut(s) 834, 1025, 1520, 1600, 1867, 2074, 2101, 2108
RseI CAYNNNNRTG 3 cut(s) 1296, 1370, 1973
Rsr2I CGGWCCG 1 cut(s) 1656
RsrII CGGWCCG 1 cut(s) 1656
SapI GCTCTTC 1 cut(s) 1991
SaqAI TTAA 7 cut(s) 165, 317, 591, 888, 1136, 1266, 2176
Sau3AI GATC 8 cut(s) 98, 454, 460, 678, 778, 936, 1084, 1872
Sau96I GGNCC 5 cut(s) 674, 1163, 1478, 1636, 1656
ScaI AGTACT 1 cut(s) 1601
SchI GAGTC 4 cut(s) 832, 1371, 1673, 2086
ScrFI CCNGG 4 cut(s) 214, 1476, 1561, 1728
SduI GDGCHC 4 cut(s) 138, 706, 926, 1673
SfaNI GCATC 6 cut(s) 335, 804, 1060, 1539, 1965, 2146
SfcI CTRYAG 1 cut(s) 279
SfuI TTCGAA 1 cut(s) 347
SinI GGWCC 3 cut(s) 674, 1478, 1656
SmiMI CAYNNNNRTG 3 cut(s) 1296, 1370, 1973
SmlI CTYRAG 2 cut(s) 1135, 1628
SmoI CTYRAG 2 cut(s) 1135, 1628
SpeI ACTAGT 1 cut(s) 586
Sse9I AATT 7 cut(s) 162, 906, 990, 1248, 1530, 1983, 2173
SseBI AGGCCT 1 cut(s) 621
SspI AATATT 1 cut(s) 80
SspMI CTAG 8 cut(s) 96, 477, 587, 782, 977, 1041, 1244, 1814
StuI AGGCCT 1 cut(s) 621
StyD4I CCNGG 4 cut(s) 212, 1474, 1559, 1726
StyI CCWWGG 1 cut(s) 476
TaaI ACNGT 8 cut(s) 133, 265, 1528, 1599, 1678, 1699, 1723, 1866
TaiI ACGT 5 cut(s) 1353, 1405, 1832, 1862, 2079
TaqI TCGA 7 cut(s) 347, 1399, 1407, 1673, 1850, 1875, 1988
TaqII GACCGA 1 cut(s) 691
TasI AATT 7 cut(s) 162, 906, 990, 1248, 1530, 1983, 2173
TatI WGTACW 5 cut(s) 833, 1024, 1599, 2100, 2107
TauI GCSGC 4 cut(s) 1164, 1712, 1715, 1752
TfiI GAWTC 3 cut(s) 962, 1376, 1852
Tru1I TTAA 7 cut(s) 165, 317, 591, 888, 1136, 1266, 2176
Tru9I TTAA 7 cut(s) 165, 317, 591, 888, 1136, 1266, 2176
TscAI CASTG 2 cut(s) 138, 1977
TseFI GTSAC 7 cut(s) 56, 785, 1051, 1830, 1860, 1945, 2041
Tsp45I GTSAC 7 cut(s) 56, 785, 1051, 1830, 1860, 1945, 2041
TspGWI ACGGA 2 cut(s) 1251, 2121
TspRI CASTG 2 cut(s) 138, 1977
Vha464I CTTAAG 1 cut(s) 1135
VneI GTGCAC 1 cut(s) 134
VpaK11BI GGWCC 3 cut(s) 674, 1478, 1656
XagI CCTNNNNNAGG 1 cut(s) 305
XapI RAATTY 1 cut(s) 906
XceI RCATGY 3 cut(s) 539, 834, 1325
XcmI CCANNNNNNNNNTGG 1 cut(s) 1390
XmaJI CCTAGG 1 cut(s) 476
XmiI GTMKAC 3 cut(s) 429, 765, 1272
XspI CTAG 8 cut(s) 96, 477, 587, 782, 977, 1041, 1244, 1814
ZraI GACGTC 2 cut(s) 1351, 1860
ZrmI AGTACT 1 cut(s) 1601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.