pycom05g32030

Ankyrin repeat-containing protein At3g12360-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000724
Physical Location & Seq
Reverse (-)
31555677 .. 31558560
2884 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32030.1

Sequence Viewer

Length: 2145 bp
ATGGAGTCCCAGAGAACGCCCACTGTTCCACCATATCAAAATGAGGAAGACGACCTAAAGAATAAGGTAGCAAGATTAGAAGAAATGAAAATCCCTTATGAACATTTCATACAGGGTAACTGGGAAGGCGTGAAGAAATACTACGAGACGTATCCGGCAAAATTGCTCAGGCCAATGACGCTGAACGGGAACAATGTACTTCACCTTGCGGCTTCTTGCAGCAGCAACTGTCAAAGAGACCTCAAATTTCTTATTGATATATGCAGCGGTTCAACCAGCAACATGAGTTGTTCTTTGAGGATTCCGAATAATGACGGAAACAATGTTCTTCACCAGGTGGCTGTGTCCGGCAGTGTGGAAGCGGCTGAGTTCTTGGTGAACAAATACAACGACCCTGTCCCAGACATTTGTCATACTGATGAAGAGAATGACACGCAGCCACTGCTGTGGACTCGGAACAAATTAGGAGAAACTCCACTGTACAGAACGGCTGCTTTCGGTCACACTAAATTGGTCAAGTTTTACTGTGACAAACTGAAGGCAGAAAGTCCGGACAATCTAATTAAGAAGCAGTTCAAGAGATCTGACAAAATCTCCATTCTTCATATTGCTGTCATCGCCCAACATTTTGAGACTGCTTATTGGTTGCTAAAGAGGTACAGGAATCTAGGAAAAAAGAGAGACGCAGAAGGATTGACAAGCCTTCAGTTGTTAGCCCAAATGCCAACTGCCCTTGGGCCACGGTTTCAAGAAAGCCAATGGAAGATGCTCATTTATCATTGCCTTCCTGATGGAGGTGATGTGGTCACACCAAATCAGAAGGATGATGTGGAGAGCAGCACGGATAGCCACCATCCCCGTCAATCCATCTTTCGGAACAAGTTTGCAGTTGTTGTGAAGGCTTACTATTCATTAAAGAAATCCCTTGCTAAAGAAGAAGGAGGCCTGCAGAGCGGCATAATATATAGGATATGGAAGGACAAGAAAAATAAAAAATCACTAAAGAAACTCATCAAATTGCTCGTCGAGGATGACCATTCTTGGTTCACTACTAACAAGGACGCAAAGCCCAAGGCTGTTTCTGTGGGGCCAGTGAATAAAGATCGTGATTCCGGTGGTGAAATAGGAAAGGAAGCAACCAAATCGGGTAAGGGGGCGAAGATTGAAGTTTACAACTCTACCCCATTGCTTATAGCAACTATCAAAGGAATTATGCCCATTGTGGAAGAGATACTTAAGCAGCATCCTCAGGCAGTCGAGCACCTTAACAACGACGAACAGAACATTTTGCATCTGGCCATTCTGAACCGTCAGACAAAGATCCTTAATCTTATTCAAAACAAACCAACTTTGAAGTCGAAGCTGAATGAGAGGATAGACCGCAATGGAAACACCATTTTGCACCATGCTGCAGATAGGAGTTACTATTCTATATCGTTGTCTCAGAAATTAATAGGCCCTGCCATGCAATTGCAAGCTGAGCTGCGCTGGTTCCTGGTAATGTTCTCTTTACCGCCTCATTACACCATTCACCACAACAAGAAGGATCAAACAGCGGAGGAGATGTTCAACTATTGGCATGACCAACTTCTGGAGGACGCACAAAAATGGGTAAAAGAAACGGCTCAGGCATGCTCAACCGTGGCGGTGCTAGTGGCCACTGTAGTCTTTGCAGCTGCCTACGCCATTCCCGGGGGTACTAATGACCAAAGTGGCCTTCCTGTTTTTCGGGACGATCCTCTCTTTTTGCTCTTCACCTGCATGGACGTTGTGGCCATCGCCTGCTCATTATCTTCTGTGGCATTCTTTCTCTCGGTCCTCTCCTCCCCTCTCGAGTACCCATATTTCGTTGACAGCATTCCAAACAAGATCTTGACAGGATTCGTCTTGCTTTTCTTCTCCATGGCAACCACCATGCTGGCCTTTGCTGCCACCATTTTGCTCTTGATTCGTGTAGAGAAGAAATGGACCAAGTCTCTACTTTACCCTATTGCTTTCTTCCCGGTCCCTCTATTCGGCCTGCTGCAGTTTCCTATGTATCAAACTTTCAAAGTCACGTTTCAGAAAATTCATGCCTGGTTTTTCAAACCCTTACTCCGCTTTACTGGCATTCCCAAGAGAAGATCAATATGGGGCACTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

715

Amino Acids

81.14

Weight (kDa)

9.09

Isoelectric Point (pI)

41.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 53 - 131 7.5e-06 Ankyrin repeats (3 copies)
PGG PF13962 536 - 647 2.3e-20 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1766
Acc36I ACCTGC 1 cut(s) 1766
AccB7I CCANNNNNTGG 1 cut(s) 1591
AccBSI CCGCTC 1 cut(s) 954
AccIII TCCGGA 1 cut(s) 550
AciI CCGC 9 cut(s) 209, 267, 362, 954, 1381, 1514, 1556, 1646, 2098
AclWI GGATC 3 cut(s) 1315, 1554, 1730
AcoI YGGCCR 3 cut(s) 1296, 1656, 1773
AcsI RAATTY 2 cut(s) 245, 2067
AcuI CTGAAG 2 cut(s) 557, 689
AdeI CACNNNGTG 1 cut(s) 337
AfaI GTAC 5 cut(s) 198, 482, 659, 1699, 1838
AfiI CCNNNNNNNGG 5 cut(s) 794, 873, 1591, 1692, 2015
AflII CTTAAG 1 cut(s) 1235
AgsI TTSAA 9 cut(s) 273, 577, 749, 1166, 1337, 1354, 1570, 2050, 2086
AjnI CCWGG 3 cut(s) 333, 1494, 2075
AjuI GAANNNNNNNTTGG 2 cut(s) 1701, 1733
AleI CACNNNNGTG 1 cut(s) 445
AloI GAACNNNNNNTCC 4 cut(s) 309, 341, 1550, 1582
AluBI AGCT 4 cut(s) 1363, 1478, 1483, 1676
AluI AGCT 4 cut(s) 1363, 1478, 1483, 1676
Alw21I GWGCWC 1 cut(s) 1263
Alw26I GTCTC 6 cut(s) 140, 231, 626, 675, 1446, 1980
AlwI GGATC 3 cut(s) 1315, 1554, 1730
AlwNI CAGNNNCTG 2 cut(s) 228, 442
Ama87I CYCGRG 2 cut(s) 1691, 1832
Aor13HI TCCGGA 1 cut(s) 550
ApoI RAATTY 2 cut(s) 245, 2067
ArsI GACNNNNNNTTYG 2 cut(s) 1008, 1040
AseI ATTAAT 1 cut(s) 1451
Asp700I GAANNNNTTC 1 cut(s) 572
AspLEI GCGC 1 cut(s) 1488
AspS9I GGNCC 6 cut(s) 737, 1088, 1457, 1816, 1968, 2005
AsuC2I CCSGG 3 cut(s) 1692, 1693, 2003
AsuHPI GGTGA 7 cut(s) 194, 323, 388, 809, 1130, 1523, 1747
AvaI CYCGRG 2 cut(s) 1691, 1832
AvaII GGWCC 3 cut(s) 1816, 1968, 2005
AxyI CCTNAGG 1 cut(s) 1248
BaeGI GKGCMC 1 cut(s) 2138
BalI TGGCCA 3 cut(s) 1298, 1658, 1775
BarI GAAGNNNNNNTAC 2 cut(s) 125, 157
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 1 cut(s) 1263
BccI CCATC 4 cut(s) 785, 861, 875, 1784
BceAI ACGGC 2 cut(s) 504, 1638
BcgI CGANNNNNNTGC 2 cut(s) 1125, 1159
BciT130I CCWGG 3 cut(s) 335, 1496, 2077
BciVI GTATCC 1 cut(s) 162
BcnI CCSGG 3 cut(s) 1692, 1693, 2003
BcoDI GTCTC 6 cut(s) 140, 231, 626, 675, 1446, 1980
BfaI CTAG 2 cut(s) 668, 1652
BfmI CTRYAG 4 cut(s) 947, 1410, 1662, 2024
BfrI CTTAAG 1 cut(s) 1235
BfuAI ACCTGC 1 cut(s) 1766
BfuI GTATCC 1 cut(s) 162
BglII AGATCT 2 cut(s) 581, 1869
BlpI GCTNAGC 1 cut(s) 1479
Bme1390I CCNGG 6 cut(s) 335, 1496, 1692, 1693, 2003, 2077
Bme18I GGWCC 3 cut(s) 1816, 1968, 2005
BmeT110I CYCGRG 2 cut(s) 1691, 1832
BmgT120I GGNCC 6 cut(s) 737, 1088, 1457, 1816, 1968, 2005
BmiI GGNNCC 3 cut(s) 1089, 1493, 2007
BmrFI CCNGG 6 cut(s) 335, 1496, 1692, 1693, 2003, 2077
BmrI ACTGGG 1 cut(s) 130
BmsI GCATC 3 cut(s) 756, 1252, 1300
BmuI ACTGGG 1 cut(s) 130
BoxI GACNNNNGTC 1 cut(s) 408
BpiI GAAGAC 1 cut(s) 54
BpmI CTGGAG 1 cut(s) 1613
Bpu10I CCTNAGC 2 cut(s) 167, 1626
Bpu1102I GCTNAGC 1 cut(s) 1479
BpuMI CCSGG 3 cut(s) 1692, 1693, 2003
BsaI GGTCTC 1 cut(s) 231
BsaJI CCNNGG 7 cut(s) 733, 740, 1071, 1641, 1691, 1692, 1902
BsaWI WCCGGW 2 cut(s) 550, 1112
BsaXI ACNNNNNCTCC 8 cut(s) 578, 608, 786, 816, 1550, 1580, 2079, 2109
Bsc4I CCNNNNNNNGG 5 cut(s) 794, 873, 1591, 1692, 2015
Bse1I ACTGG 3 cut(s) 125, 1091, 2110
Bse21I CCTNAGG 1 cut(s) 1248
Bse3DI GCAATG 3 cut(s) 778, 1184, 1390
BseAI TCCGGA 1 cut(s) 550
BseBI CCWGG 3 cut(s) 335, 1496, 2077
BseDI CCNNGG 7 cut(s) 733, 740, 1071, 1641, 1691, 1692, 1902
BseGI GGATG 4 cut(s) 829, 853, 1036, 1243
BseLI CCNNNNNNNGG 5 cut(s) 794, 873, 1591, 1692, 2015
BseMI GCAATG 3 cut(s) 778, 1184, 1390
BseMII CTCAG 7 cut(s) 181, 357, 1262, 1457, 1470, 1640, 2130
BseNI ACTGG 3 cut(s) 125, 1091, 2110
BseRI GAGGAG 2 cut(s) 1574, 1813
BseSI GKGCMC 1 cut(s) 2138
BsiHKAI GWGCWC 1 cut(s) 1263
BsiHKCI CYCGRG 2 cut(s) 1691, 1832
BsiSI CCGG 6 cut(s) 155, 348, 551, 1113, 1692, 2003
BslFI GGGAC 3 cut(s) 383, 1745, 1991
BslI CCNNNNNNNGG 5 cut(s) 794, 873, 1591, 1692, 2015
BsmAI GTCTC 6 cut(s) 140, 231, 626, 675, 1446, 1980
BsmBI CGTCTC 2 cut(s) 140, 675
BsmFI GGGAC 3 cut(s) 383, 1745, 1991
BsmI GAATGC 3 cut(s) 1802, 1857, 2109
Bso31I GGTCTC 1 cut(s) 231
BsoBI CYCGRG 2 cut(s) 1691, 1832
Bsp1286I GDGCHC 2 cut(s) 1263, 2138
Bsp13I TCCGGA 1 cut(s) 550
Bsp1407I TGTACA 1 cut(s) 480
Bsp143I GATC 7 cut(s) 581, 1102, 1320, 1546, 1735, 1869, 2123
Bsp1720I GCTNAGC 1 cut(s) 1479
Bsp19I CCATGG 1 cut(s) 1902
BspACI CCGC 9 cut(s) 209, 267, 362, 954, 1381, 1514, 1556, 1646, 2098
BspCNI CTCAG 7 cut(s) 180, 358, 1261, 1456, 1471, 1639, 2131
BspEI TCCGGA 1 cut(s) 550
BspLI GGNNCC 3 cut(s) 1089, 1493, 2007
BspMAI CTGCAG 3 cut(s) 951, 1414, 2028
BspMI ACCTGC 1 cut(s) 1766
BspPI GGATC 3 cut(s) 1315, 1554, 1730
BspQI GCTCTTC 1 cut(s) 1757
BspTI CTTAAG 1 cut(s) 1235
BspTNI GGTCTC 1 cut(s) 231
BsrBI CCGCTC 1 cut(s) 954
BsrDI GCAATG 3 cut(s) 778, 1184, 1390
BsrGI TGTACA 1 cut(s) 480
BsrI ACTGG 3 cut(s) 125, 1091, 2110
BssECI CCNNGG 7 cut(s) 733, 740, 1071, 1641, 1691, 1692, 1902
BssMI GATC 7 cut(s) 581, 1102, 1320, 1546, 1735, 1869, 2123
BssT1I CCWWGG 3 cut(s) 733, 1071, 1902
Bst2UI CCWGG 3 cut(s) 335, 1496, 2077
Bst4CI ACNGT 8 cut(s) 25, 230, 480, 527, 744, 1310, 1642, 1663
Bst6I CTCTTC 3 cut(s) 417, 1221, 1757
BstAFI CTTAAG 1 cut(s) 1235
BstAPI GCANNNNNTGC 1 cut(s) 442
BstAUI TGTACA 1 cut(s) 480
BstC8I GCNNGC 6 cut(s) 947, 1476, 1633, 1783, 1920, 2021
BstDEI CTNAG 7 cut(s) 167, 366, 1248, 1443, 1479, 1626, 2139
BstDSI CCRYGG 3 cut(s) 740, 1641, 1902
BstENI CCTNNNNNAGG 1 cut(s) 792
BstF5I GGATG 4 cut(s) 829, 853, 1036, 1243
BstHHI GCGC 1 cut(s) 1488
BstKTI GATC 7 cut(s) 584, 1105, 1323, 1549, 1738, 1872, 2126
BstMAI GTCTC 6 cut(s) 140, 231, 626, 675, 1446, 1980
BstMBI GATC 7 cut(s) 581, 1102, 1320, 1546, 1735, 1869, 2123
BstMWI GCNNNNNNNGC 9 cut(s) 178, 442, 617, 846, 951, 1480, 1682, 1928, 2106
BstNI CCWGG 3 cut(s) 335, 1496, 2077
BstNSI RCATGY 1 cut(s) 1635
BstPAI GACNNNNGTC 1 cut(s) 408
BstSCI CCNGG 6 cut(s) 333, 1494, 1690, 1691, 2001, 2075
BstSFI CTRYAG 4 cut(s) 947, 1410, 1662, 2024
BstSLI GKGCMC 1 cut(s) 2138
BstV2I GAAGAC 1 cut(s) 54
BstX2I RGATCY 3 cut(s) 581, 1320, 1869
BstXI CCANNNNNNTGG 2 cut(s) 447, 1918
BstYI RGATCY 3 cut(s) 581, 1320, 1869
Bsu36I CCTNAGG 1 cut(s) 1248
BsuI GTATCC 1 cut(s) 162
BtgI CCRYGG 3 cut(s) 740, 1641, 1902
BtgZI GCGATG 2 cut(s) 601, 1762
BtsCI GGATG 4 cut(s) 829, 853, 1036, 1243
BtsI GCAGTG 2 cut(s) 358, 440
BtsIMutI CAGTG 7 cut(s) 21, 358, 440, 476, 1098, 1659, 2136
BveI ACCTGC 1 cut(s) 1766
Cac8I GCNNGC 6 cut(s) 947, 1476, 1633, 1783, 1920, 2021
CaiI CAGNNNCTG 2 cut(s) 228, 442
CfoI GCGC 1 cut(s) 1488
Cfr13I GGNCC 6 cut(s) 737, 1088, 1457, 1816, 1968, 2005
Cfr9I CCCGGG 1 cut(s) 1691
CseI GACGC 4 cut(s) 187, 692, 1070, 1607
CsiI ACCWGGT 1 cut(s) 333
Csp6I GTAC 5 cut(s) 197, 481, 658, 1698, 1837
CviAII CATG 9 cut(s) 283, 1406, 1465, 1580, 1632, 1762, 1903, 1915, 2072
CviQI GTAC 5 cut(s) 197, 481, 658, 1698, 1837
DdeI CTNAG 7 cut(s) 167, 366, 1248, 1443, 1479, 1626, 2139
DpnI GATC 7 cut(s) 583, 1104, 1322, 1548, 1737, 1871, 2125
DpnII GATC 7 cut(s) 581, 1102, 1320, 1546, 1735, 1869, 2123
DraIII CACNNNGTG 1 cut(s) 337
EaeI YGGCCR 3 cut(s) 1296, 1656, 1773
Eam1104I CTCTTC 3 cut(s) 417, 1221, 1757
EarI CTCTTC 3 cut(s) 417, 1221, 1757
Eco130I CCWWGG 3 cut(s) 733, 1071, 1902
Eco147I AGGCCT 1 cut(s) 945
Eco31I GGTCTC 1 cut(s) 231
Eco47I GGWCC 3 cut(s) 1816, 1968, 2005
Eco57I CTGAAG 2 cut(s) 557, 689
Eco81I CCTNAGG 1 cut(s) 1248
Eco88I CYCGRG 2 cut(s) 1691, 1832
EcoNI CCTNNNNNAGG 1 cut(s) 792
EcoO109I RGGNCCY 1 cut(s) 1457
EcoRII CCWGG 3 cut(s) 333, 1494, 2075
EcoT14I CCWWGG 3 cut(s) 733, 1071, 1902
ErhI CCWWGG 3 cut(s) 733, 1071, 1902
Esp3I CGTCTC 2 cut(s) 140, 675
FaeI CATG 9 cut(s) 286, 1409, 1468, 1583, 1635, 1765, 1906, 1918, 2075
FalI AAGNNNNNCTT 2 cut(s) 1218, 1250
FaqI GGGAC 3 cut(s) 383, 1745, 1991
FatI CATG 9 cut(s) 282, 1405, 1464, 1579, 1631, 1761, 1902, 1914, 2071
FokI GGATG 4 cut(s) 836, 840, 1043, 1230
FspBI CTAG 2 cut(s) 668, 1652
GlaI GCGC 1 cut(s) 1487
GsuI CTGGAG 1 cut(s) 1613
HapII CCGG 6 cut(s) 155, 348, 551, 1113, 1692, 2003
HgaI GACGC 4 cut(s) 187, 692, 1070, 1607
HhaI GCGC 1 cut(s) 1488
Hin1II CATG 9 cut(s) 286, 1409, 1468, 1583, 1635, 1765, 1906, 1918, 2075
Hin6I GCGC 1 cut(s) 1486
HinP1I GCGC 1 cut(s) 1486
HincII GTYRAC 1 cut(s) 1852
HindII GTYRAC 1 cut(s) 1852
HinfI GANTC 7 cut(s) 5, 301, 451, 664, 1109, 1881, 1948
HpaII CCGG 6 cut(s) 155, 348, 551, 1113, 1692, 2003
HphI GGTGA 7 cut(s) 194, 323, 388, 809, 1130, 1523, 1747
Hpy166II GTNNAC 5 cut(s) 379, 450, 1047, 1171, 1852
Hpy188I TCNGA 9 cut(s) 306, 456, 586, 819, 876, 1305, 1314, 1446, 2064
Hpy8I GTNNAC 5 cut(s) 379, 450, 1047, 1171, 1852
Hpy99I CGWCG 2 cut(s) 1028, 1277
HpyCH4III ACNGT 8 cut(s) 25, 230, 480, 527, 744, 1310, 1642, 1663
HpyCH4IV ACGT 3 cut(s) 149, 1767, 2057
HpyF10VI GCNNNNNNNGC 9 cut(s) 178, 442, 617, 846, 951, 1480, 1682, 1928, 2106
HpyF3I CTNAG 7 cut(s) 167, 366, 1248, 1443, 1479, 1626, 2139
HpySE526I ACGT 3 cut(s) 149, 1767, 2057
Hsp92II CATG 9 cut(s) 286, 1409, 1468, 1583, 1635, 1765, 1906, 1918, 2075
HspAI GCGC 1 cut(s) 1486
Kpn2I TCCGGA 1 cut(s) 550
Kzo9I GATC 7 cut(s) 581, 1102, 1320, 1546, 1735, 1869, 2123
LguI GCTCTTC 1 cut(s) 1757
LweI GCATC 3 cut(s) 756, 1252, 1300
MabI ACCWGGT 1 cut(s) 333
MaeI CTAG 2 cut(s) 668, 1652
MaeII ACGT 3 cut(s) 149, 1767, 2057
MaeIII GTNAC 6 cut(s) 116, 500, 527, 805, 1421, 2053
MalI GATC 7 cut(s) 583, 1104, 1322, 1548, 1737, 1871, 2125
MbiI CCGCTC 1 cut(s) 954
MboI GATC 7 cut(s) 581, 1102, 1320, 1546, 1735, 1869, 2123
MfeI CAATTG 1 cut(s) 1469
MflI RGATCY 3 cut(s) 581, 1320, 1869
MhlI GDGCHC 2 cut(s) 1263, 2138
MlsI TGGCCA 3 cut(s) 1298, 1658, 1775
MluNI TGGCCA 3 cut(s) 1298, 1658, 1775
MlyI GAGTC 2 cut(s) 14, 445
Mox20I TGGCCA 3 cut(s) 1298, 1658, 1775
MroI TCCGGA 1 cut(s) 550
MroXI GAANNNNTTC 1 cut(s) 572
MscI TGGCCA 3 cut(s) 1298, 1658, 1775
MseI TTAA 6 cut(s) 564, 914, 1236, 1266, 1326, 1451
MslI CAYNNNNRTG 4 cut(s) 417, 445, 1606, 1760
Msp20I TGGCCA 3 cut(s) 1298, 1658, 1775
MspA1I CMGCKG 3 cut(s) 267, 1556, 1676
MspCI CTTAAG 1 cut(s) 1235
MspI CCGG 6 cut(s) 155, 348, 551, 1113, 1692, 2003
MspR9I CCNGG 6 cut(s) 335, 1496, 1692, 1693, 2003, 2077
MunI CAATTG 1 cut(s) 1469
Mva1269I GAATGC 3 cut(s) 1802, 1857, 2109
MvaI CCWGG 3 cut(s) 335, 1496, 2077
MwoI GCNNNNNNNGC 9 cut(s) 178, 442, 617, 846, 951, 1480, 1682, 1928, 2106
NciI CCSGG 3 cut(s) 1692, 1693, 2003
NcoI CCATGG 1 cut(s) 1902
NdeII GATC 7 cut(s) 581, 1102, 1320, 1546, 1735, 1869, 2123
NlaIII CATG 9 cut(s) 286, 1409, 1468, 1583, 1635, 1765, 1906, 1918, 2075
NlaIV GGNNCC 3 cut(s) 1089, 1493, 2007
NmuCI GTSAC 4 cut(s) 500, 527, 805, 2053
NspI RCATGY 1 cut(s) 1635
OliI CACNNNNGTG 1 cut(s) 445
PaeI GCATGC 1 cut(s) 1635
PaeR7I CTCGAG 1 cut(s) 1832
PaqCI CACCTGC 1 cut(s) 1766
PceI AGGCCT 1 cut(s) 945
PciSI GCTCTTC 1 cut(s) 1757
PctI GAATGC 3 cut(s) 1802, 1857, 2109
PdmI GAANNNNTTC 1 cut(s) 572
PfeI GAWTC 5 cut(s) 301, 664, 1109, 1881, 1948
PflFI GACNNNGTC 2 cut(s) 395, 1972
PflMI CCANNNNNTGG 1 cut(s) 1591
PleI GAGTC 2 cut(s) 13, 445
PpsI GAGTC 2 cut(s) 13, 445
PshAI GACNNNNGTC 1 cut(s) 408
PshBI ATTAAT 1 cut(s) 1451
Psp6I CCWGG 3 cut(s) 333, 1494, 2075
PspGI CCWGG 3 cut(s) 333, 1494, 2075
PspN4I GGNNCC 3 cut(s) 1089, 1493, 2007
PspPI GGNCC 6 cut(s) 737, 1088, 1457, 1816, 1968, 2005
PsrI GAACNNNNNNTAC 2 cut(s) 93, 125
PstI CTGCAG 3 cut(s) 951, 1414, 2028
PstNI CAGNNNCTG 2 cut(s) 228, 442
PsuI RGATCY 3 cut(s) 581, 1320, 1869
PsyI GACNNNGTC 2 cut(s) 395, 1972
PvuII CAGCTG 1 cut(s) 1676
RsaI GTAC 5 cut(s) 198, 482, 659, 1699, 1838
RsaNI GTAC 5 cut(s) 197, 481, 658, 1698, 1837
RseI CAYNNNNRTG 4 cut(s) 417, 445, 1606, 1760
SapI GCTCTTC 1 cut(s) 1757
SaqAI TTAA 6 cut(s) 564, 914, 1236, 1266, 1326, 1451
Sau3AI GATC 7 cut(s) 581, 1102, 1320, 1546, 1735, 1869, 2123
Sau96I GGNCC 6 cut(s) 737, 1088, 1457, 1816, 1968, 2005
SchI GAGTC 2 cut(s) 14, 445
ScrFI CCNGG 6 cut(s) 335, 1496, 1692, 1693, 2003, 2077
SduI GDGCHC 2 cut(s) 1263, 2138
SexAI ACCWGGT 1 cut(s) 333
SfaNI GCATC 3 cut(s) 756, 1252, 1300
SfcI CTRYAG 4 cut(s) 947, 1410, 1662, 2024
Sfr274I CTCGAG 1 cut(s) 1832
SinI GGWCC 3 cut(s) 1816, 1968, 2005
SlaI CTCGAG 1 cut(s) 1832
SmaI CCCGGG 1 cut(s) 1693
SmiMI CAYNNNNRTG 4 cut(s) 417, 445, 1606, 1760
SmlI CTYRAG 2 cut(s) 1235, 1832
SmoI CTYRAG 2 cut(s) 1235, 1832
SphI GCATGC 1 cut(s) 1635
SseBI AGGCCT 1 cut(s) 945
SsiI CCGC 9 cut(s) 209, 267, 362, 954, 1381, 1514, 1556, 1646, 2098
SspMI CTAG 2 cut(s) 668, 1652
StuI AGGCCT 1 cut(s) 945
StyD4I CCNGG 6 cut(s) 333, 1494, 1690, 1691, 2001, 2075
StyI CCWWGG 3 cut(s) 733, 1071, 1902
TaaI ACNGT 8 cut(s) 25, 230, 480, 527, 744, 1310, 1642, 1663
TaiI ACGT 3 cut(s) 152, 1770, 2060
TaqI TCGA 4 cut(s) 1026, 1257, 1358, 1833
TaqII GACCGA 2 cut(s) 488, 1804
TatI WGTACW 2 cut(s) 196, 480
TauI GCSGC 3 cut(s) 212, 365, 957
TfiI GAWTC 5 cut(s) 301, 664, 1109, 1881, 1948
Tru1I TTAA 6 cut(s) 564, 914, 1236, 1266, 1326, 1451
Tru9I TTAA 6 cut(s) 564, 914, 1236, 1266, 1326, 1451
TscAI CASTG 7 cut(s) 28, 358, 447, 483, 1098, 1666, 2143
TseFI GTSAC 4 cut(s) 500, 527, 805, 2053
Tsp45I GTSAC 4 cut(s) 500, 527, 805, 2053
TspDTI ATGAA 7 cut(s) 97, 101, 114, 435, 593, 900, 2060
TspGWI ACGGA 2 cut(s) 330, 857
TspMI CCCGGG 1 cut(s) 1691
TspRI CASTG 7 cut(s) 28, 358, 447, 483, 1098, 1666, 2143
Tth111I GACNNNGTC 2 cut(s) 395, 1972
Van91I CCANNNNNTGG 1 cut(s) 1591
Vha464I CTTAAG 1 cut(s) 1235
VpaK11BI GGWCC 3 cut(s) 1816, 1968, 2005
VspI ATTAAT 1 cut(s) 1451
XagI CCTNNNNNAGG 1 cut(s) 792
XapI RAATTY 2 cut(s) 245, 2067
XceI RCATGY 1 cut(s) 1635
XhoI CTCGAG 1 cut(s) 1832
XmaI CCCGGG 1 cut(s) 1691
XmnI GAANNNNTTC 1 cut(s) 572
XspI CTAG 2 cut(s) 668, 1652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.