pycom05g01740

Ankyrin repeat-containing protein At3g12360-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000724
Physical Location & Seq
Forward (+)
2127282 .. 2133077
5796 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g01740.13

Sequence Viewer

Length: 3258 bp
ATGGCAATAAAAGACAGAAATTGTAAACAAACTCTCCTACAGAATTACAAATATCCTAATACTTGTGACCGAAGCAATCACAGGATCATAACACGCAGACTCTACGTTCAAAGAAATTTGGGCAGCAATGACACCCGTCACTCGTTCATTTCGAATTCAGAGAGGAGTAGTAATGACAACAGTTGCTTGCTAATCTATTGCTACCGCACACAGCTAAGTTTTTTCTTATTTTTACGACATGGCTTTGGCGTCAAGAAGAGAATGCTTTTAGAATTGTGTATTATATGTCTATTCATCTCTCAAGAAGGAATTCATAAGATGATACAATCATATCAAATAAGAATGTACAAAAAGTCAACAAAATTAACCTCGATTAATTACTTTTTCATTGAATTAAGATTTCCTCTAAAGGGTAATGCTAGGGAGACTAAATTTCTAAACCAAATGATGTGGATGTTCAAAAATTTGGTCTCCCTCCTAAAAACCAAGAACTCAAAAGAAAAAGTAACGAAGCAAGCGAAGCAAGCGAAGATTTTTGTCACCTTAACTTCTCAAGGAAACAAAGCAAAGCTAGTTATGTTTTTTTCTTACTTTTCTTCACTTCTAATCTTCTTTTTGGATTTGAATCATAATTACTTATCTTCTTTCTCTGCGATACCAGCCTTCAAAACCGAAACCATGTCGATCAATGCTAACACACTTTACCATAAGGCTGGGAAGAAGACAGCTGTTAACTTTGAATCTTTTCAAAACTCTCATTTTTCAGCTTTTGACATTATATATAAGCAACGTCTGAAATTATATAGTTTGAAAGCATCTAATTCTTTGATGAAAATTGCATATTTAATAATCGTACAACTGTACATAAAAAAGGCCAGAATATGGACTATCAATGAAGCAAATGACTTACATGCAGTACTGTCACTTTGTAAACTGTACGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATGGAAGAGAGAGAGATGGAGAGGTTGAAACAGAGAGAAAAAGATGAGAAAATAGAAAGAGAGGGAGAGGCACTGAGTCGAACAAAAGGGCCATATCAATGGGCCATGTACGACGACTGGGAACGCCTGAAACAATACTATAAGGGAAACTGCCAAGAGGTATTCAGTCCATTGACAACGAACGACGACACTGTACTTCACGTCGTAGCCTTTGCCGGGCGGAGCGACGTGCTTGAATTCTTGATTTCACTTGTGACAACTGCAACCAACAACGACCTTCGGAATTTACGGAATGCCTTGGTGCTGGAGAATAGCCACGGGAACACCATCCTCCACGAGGTGGCGGCGTCGGGAAACCTGGAAGCGGCAATGCTGTTGGTAAGCCACGATAAACGCACAAGGAGAGAGCTAATTGCTAGACGGGATGCTGACCAAGACAATGAGTATGAGCCGGATGTTGATGCTAGCTCTAGACGTATTGTGGAGATTGAGAACAAGTTAGGAGAAACGCCGTTCTACAGGGCAGCTTCTTACGGTCAGACAAAGTTAGTCGACTATTTCAACAAACAAAATGTGAATATAGAGCACCACTTAACCAGAAACGTTGATCGCATGTCCGTTCTTCATATCGCTGTCATTAACCAACATTTTGAAACTGCTGTTTGGTTACTTGAAAAGTACCCATTTCTTGCAAAAGAAAGGGAAGACCACGGTTTCACAAGCCTTCAGTTGCTAGCCCAGATGCCATCTGCCTTCCAAGTTTACTATCGCAAAAGCTTATGGAAGATGCTTATTCATAAATGCCTCTCTGAAGATGATAATGTTGATGGGGACGGTGATATAGAGAGCAGATGGATTCCACAGTTTTCCACGGCTTGCTTTTCGAAGACGCTCTCAAGATGGCCTCGAGTGTTTTCGATCGTGAAGCAAATAAAAAAAGAAAAATCACTATCGGAGCTCACTCATCTGCTCGTAGAGAAAGACTATTCGTGGGTAGAAAGTAAGAAAACAGAGAAAGACAAGACAATTTCGTTGGTGTCCTTACAGAAAGATAGTGCTGCTATTGGTGGTGATGGAAGAGAAAGAAAAAAATATACTATTTATAACTACACCCCACTGCTTATAGCAACTAGCACCGGAATATTACCTCTCGTGAAAAAGATGTTTGAGGTACATCCTCAGGCGGTTGAGGCGCACGATATCGACAATCAACAAAACATTTTGCATATGTCTATTAAGCACCGTCAGTTGCAGATATTTAGGCTTGTGAAGAGAAGCAGAGCGATAACGTCGAGGTTAACTTCGAGGATTGACAGTGATGGAAATACCATATTGCATCGAGCTGCAGATATGACATATTACTCTCCAGACACTCAAAGTGTAAGTGGTCCTGCGTTGCAATTGCAAGAAGAATTACGTTGGATGCTGCGCGTGAGAAAGATAATGCCTCCTCATTACATCATGCACCGCAACAACATGGGTATGACAGCGGAGGAGCTCTTCAATAGTGAGCATGCAAAGCTTCTTCAGTCGGCACAAACATGGATAAGAGGCACTGCTCAGTCATGCTCGGCCGTGGCAGCTCTAGTGGCCACTGTGGTCTTTGCAGCCGCCTACACTGCTCCCGGGGGTTACAGTTCAGGCGGTGTTCCTGTTCTCCGTCATTCTCCTTTCTTCACTATCTTTATCATCACAGACGTTGTCTCACTCATCAGCTCATTGTCTTCTCTCATCACGTTTCTCTCGATCTTGACATCTCCGTTTGAGTTCAAAAACTTCCATCAATCTCTTCCGCTCAGGCTGTTGAAGTTTACAAACTCTATCACACCTCTCACTTTCACTCTCAATAAAATTGGACTAACAAATGGAGAAGGGATAGGAAATAACAATTTACAAACTGATGAAAAAAACAGAAGCTTACAAGCTTATGAACCTGAGAGAATACAATCAGAAAATGGGATTCGGATGGATTGCCTAAAAAACAATAAGACAACACATGCAATAAGAGATGGTGTTCCAGCCGTTCAAACACATGCAGCACCAGCTGCTAGACACTTTTACACACATGCTCTGCATCATTCCTTGCTGTCTTTCAACACCTTTCGGCTAGCACATGGAGGAGACTTTTTTACAACCTGTTTTGATTTGAATTTCCAACACACCTCCTCAAATCAAAACGCCTTCATTCCTAGCATTTCACGCAGTAGCCGGAATGATTCAATTGGCAATGGCTTTGTGAAGATGTCTGCCACTTGTTCCTTGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

1086

Amino Acids

125.12

Weight (kDa)

9.43

Isoelectric Point (pI)

45.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2094
AccB7I CCANNNNNTGG 2 cut(s) 882, 1330
AccBSI CCGCTC 1 cut(s) 2785
AccI GTMKAC 1 cut(s) 1542
AccII CGCG 1 cut(s) 2421
AclI AACGTT 1 cut(s) 1593
AclWI GGATC 1 cut(s) 92
AcoI YGGCCR 2 cut(s) 2562, 2580
AcsI RAATTY 8 cut(s) 115, 154, 309, 431, 463, 1226, 1273, 3139
AcuI CTGAAG 3 cut(s) 1700, 1821, 2501
AcyI GRCGYC 2 cut(s) 249, 1337
AdeI CACNNNGTG 1 cut(s) 1330
AfaI GTAC 9 cut(s) 347, 855, 863, 918, 938, 1100, 1185, 1670, 2163
AfiI CCNNNNNNNGG 6 cut(s) 410, 882, 1206, 1327, 1330, 1354
AjiI CACGTC 2 cut(s) 1192, 1219
AjnI CCWGG 1 cut(s) 1347
Alw21I GWGCWC 3 cut(s) 1578, 1950, 2490
Alw26I GTCTC 4 cut(s) 419, 475, 2698, 3105
AlwI GGATC 1 cut(s) 92
Ama87I CYCGRG 2 cut(s) 1896, 2615
AoxI GGCC 6 cut(s) 873, 1079, 1092, 1892, 2562, 2580
ApeKI GCWGC 9 cut(s) 123, 1514, 2048, 2333, 2416, 2570, 2597, 3026, 3035
ApoI RAATTY 8 cut(s) 115, 154, 309, 431, 463, 1226, 1273, 3139
ArsI GACNNNNNNTTYG 2 cut(s) 453, 485
AseI ATTAAT 1 cut(s) 375
Asp700I GAANNNNTTC 2 cut(s) 309, 744
AspLEI GCGC 2 cut(s) 2185, 2421
AspS9I GGNCC 3 cut(s) 1079, 1092, 2378
AsuC2I CCSGG 3 cut(s) 1207, 2616, 2617
AsuHPI GGTGA 3 cut(s) 532, 1838, 2072
AsuII TTCGAA 2 cut(s) 152, 1874
AsuNHI GCTAGC 3 cut(s) 1454, 1723, 3097
AvaI CYCGRG 2 cut(s) 1896, 2615
AvaII GGWCC 1 cut(s) 2378
AxyI CCTNAGG 1 cut(s) 2169
BalI TGGCCA 1 cut(s) 2582
BanII GRGCYC 2 cut(s) 1950, 2490
BauI CACGAG 2 cut(s) 1325, 2141
BbsI GAAGAC 4 cut(s) 728, 1701, 1883, 2706
Bbv12I GWGCWC 3 cut(s) 1578, 1950, 2490
BbvI GCAGC 9 cut(s) 135, 1526, 2035, 2320, 2403, 2582, 2609, 3022, 3038
BceAI ACGGC 4 cut(s) 1486, 1878, 2549, 2996
BciT130I CCWGG 1 cut(s) 1349
BcnI CCSGG 3 cut(s) 1207, 2616, 2617
BcoDI GTCTC 4 cut(s) 419, 475, 2698, 3105
BfmI CTRYAG 3 cut(s) 38, 1507, 2334
BmcAI AGTACT 1 cut(s) 918
Bme1390I CCNGG 4 cut(s) 1207, 1349, 2616, 2617
Bme18I GGWCC 1 cut(s) 2378
BmeT110I CYCGRG 2 cut(s) 1896, 2615
BmgBI CACGTC 2 cut(s) 1192, 1219
BmgT120I GGNCC 3 cut(s) 1079, 1092, 2378
BmrFI CCNGG 4 cut(s) 1207, 1349, 2616, 2617
BmrI ACTGGG 1 cut(s) 1117
BmsI GCATC 8 cut(s) 824, 1405, 1441, 1722, 1767, 2335, 2403, 3073
BmtI GCTAGC 3 cut(s) 1458, 1727, 3101
BmuI ACTGGG 1 cut(s) 1117
BoxI GACNNNNGTC 1 cut(s) 135
BpiI GAAGAC 4 cut(s) 728, 1701, 1883, 2706
BpmI CTGGAG 2 cut(s) 1316, 2340
Bpu10I CCTNAGC 1 cut(s) 2786
Bpu14I TTCGAA 2 cut(s) 152, 1874
BpuEI CTTGAG 3 cut(s) 285, 537, 1870
BpuMI CCSGG 3 cut(s) 1207, 2616, 2617
BsaBI GATNNNNATC 1 cut(s) 624
BsaHI GRCGYC 2 cut(s) 249, 1337
BsaI GGTCTC 1 cut(s) 475
BsaJI CCNNGG 8 cut(s) 1287, 1306, 1699, 1860, 2565, 2615, 2616, 3249
BsaWI WCCGGW 1 cut(s) 2126
BsaXI ACNNNNNCTCC 2 cut(s) 18, 48
Bsc4I CCNNNNNNNGG 6 cut(s) 410, 882, 1206, 1327, 1330, 1354
Bse1I ACTGG 1 cut(s) 1112
Bse21I CCTNAGG 1 cut(s) 2169
Bse3DI GCAATG 3 cut(s) 133, 1365, 3223
Bse8I GATNNNNATC 1 cut(s) 624
BseBI CCWGG 1 cut(s) 1349
BseDI CCNNGG 8 cut(s) 1287, 1306, 1699, 1860, 2565, 2615, 2616, 3249
BseGI GGATG 7 cut(s) 459, 1317, 1420, 1450, 2164, 2418, 2961
BseJI GATNNNNATC 1 cut(s) 624
BseLI CCNNNNNNNGG 6 cut(s) 410, 882, 1206, 1327, 1330, 1354
BseMI GCAATG 3 cut(s) 133, 1365, 3223
BseMII CTCAG 5 cut(s) 1055, 2183, 2564, 2800, 2916
BseNI ACTGG 1 cut(s) 1112
BseRI GAGGAG 5 cut(s) 178, 2430, 2498, 3123, 3145
BseX3I CGGCCG 1 cut(s) 2562
BseXI GCAGC 9 cut(s) 135, 1526, 2035, 2320, 2403, 2582, 2609, 3022, 3038
BseYI CCCAGC 1 cut(s) 713
Bsh1236I CGCG 1 cut(s) 2421
Bsh1285I CGRYCG 2 cut(s) 1911, 2565
BshFI GGCC 6 cut(s) 875, 1081, 1094, 1894, 2564, 2582
BsiEI CGRYCG 2 cut(s) 1911, 2565
BsiHKAI GWGCWC 3 cut(s) 1578, 1950, 2490
BsiHKCI CYCGRG 2 cut(s) 1896, 2615
BsiSI CCGG 5 cut(s) 1206, 1442, 2127, 2616, 3199
BslFI GGGAC 1 cut(s) 1835
BslI CCNNNNNNNGG 6 cut(s) 410, 882, 1206, 1327, 1330, 1354
BsmAI GTCTC 4 cut(s) 419, 475, 2698, 3105
BsmFI GGGAC 1 cut(s) 1835
BsmI GAATGC 2 cut(s) 267, 1288
BsnI GGCC 6 cut(s) 875, 1081, 1094, 1894, 2564, 2582
Bso31I GGTCTC 1 cut(s) 475
BsoBI CYCGRG 2 cut(s) 1896, 2615
Bsp119I TTCGAA 2 cut(s) 152, 1874
Bsp1286I GDGCHC 3 cut(s) 1578, 1950, 2490
Bsp1407I TGTACA 2 cut(s) 345, 861
Bsp143I GATC 5 cut(s) 84, 684, 1597, 1908, 2736
BspANI GGCC 6 cut(s) 875, 1081, 1094, 1894, 2564, 2582
BspCNI CTCAG 5 cut(s) 1056, 2182, 2563, 2799, 2917
BspFNI CGCG 1 cut(s) 2421
BspMAI CTGCAG 1 cut(s) 2338
BspOI GCTAGC 3 cut(s) 1458, 1727, 3101
BspPI GGATC 1 cut(s) 92
BspQI GCTCTTC 1 cut(s) 2495
BspT104I TTCGAA 2 cut(s) 152, 1874
BspTNI GGTCTC 1 cut(s) 475
BsrBI CCGCTC 1 cut(s) 2785
BsrDI GCAATG 3 cut(s) 133, 1365, 3223
BsrGI TGTACA 2 cut(s) 345, 861
BsrI ACTGG 1 cut(s) 1112
BssECI CCNNGG 8 cut(s) 1287, 1306, 1699, 1860, 2565, 2615, 2616, 3249
BssMI GATC 5 cut(s) 84, 684, 1597, 1908, 2736
BssNI GRCGYC 2 cut(s) 249, 1337
BssSI CACGAG 2 cut(s) 1325, 2141
BssT1I CCWWGG 2 cut(s) 1287, 3249
Bst2BI CACGAG 2 cut(s) 1325, 2141
Bst2UI CCWGG 1 cut(s) 1349
Bst6I CTCTTC 6 cut(s) 251, 990, 2062, 2255, 2495, 2784
BstACI GRCGYC 2 cut(s) 249, 1337
BstAPI GCANNNNNTGC 1 cut(s) 3035
BstAUI TGTACA 2 cut(s) 345, 861
BstBI TTCGAA 2 cut(s) 152, 1874
BstC8I GCNNGC 8 cut(s) 188, 516, 525, 1456, 1725, 1867, 2505, 3099
BstDEI CTNAG 6 cut(s) 215, 1064, 2169, 2550, 2786, 2925
BstDSI CCRYGG 4 cut(s) 1306, 1699, 1860, 2565
BstENI CCTNNNNNAGG 1 cut(s) 1325
BstF5I GGATG 7 cut(s) 459, 1317, 1420, 1450, 2164, 2418, 2961
BstFNI CGCG 1 cut(s) 2421
BstHHI GCGC 2 cut(s) 2185, 2421
BstKTI GATC 5 cut(s) 87, 687, 1600, 1911, 2739
BstMAI GTCTC 4 cut(s) 419, 475, 2698, 3105
BstMBI GATC 5 cut(s) 84, 684, 1597, 1908, 2736
BstMCI CGRYCG 2 cut(s) 1911, 2565
BstNI CCWGG 1 cut(s) 1349
BstNSI RCATGY 6 cut(s) 914, 1606, 2507, 2990, 3026, 3059
BstPAI GACNNNNGTC 1 cut(s) 135
BstSCI CCNGG 4 cut(s) 1205, 1347, 2614, 2615
BstSFI CTRYAG 3 cut(s) 38, 1507, 2334
BstUI CGCG 1 cut(s) 2421
BstV1I GCAGC 9 cut(s) 135, 1526, 2035, 2320, 2403, 2582, 2609, 3022, 3038
BstV2I GAAGAC 4 cut(s) 728, 1701, 1883, 2706
BstXI CCANNNNNNTGG 2 cut(s) 713, 1089
BstZI CGGCCG 1 cut(s) 2562
Bsu36I CCTNAGG 1 cut(s) 2169
BsuRI GGCC 6 cut(s) 875, 1081, 1094, 1894, 2564, 2582
BtgI CCRYGG 4 cut(s) 1306, 1699, 1860, 2565
BtrI CACGTC 2 cut(s) 1192, 1219
BtsCI GGATG 7 cut(s) 459, 1317, 1420, 1450, 2164, 2418, 2961
BtsI GCAGTG 3 cut(s) 2105, 2544, 2607
BtsIMutI CAGTG 7 cut(s) 1061, 1179, 2105, 2311, 2544, 2583, 2607
Cac8I GCNNGC 8 cut(s) 188, 516, 525, 1456, 1725, 1867, 2505, 3099
CfoI GCGC 2 cut(s) 2185, 2421
Cfr13I GGNCC 3 cut(s) 1079, 1092, 2378
Cfr9I CCCGGG 1 cut(s) 2615
CseI GACGC 3 cut(s) 238, 1326, 1888
Csp6I GTAC 9 cut(s) 346, 854, 862, 917, 937, 1099, 1184, 1669, 2162
CspCI CAANNNNNGTGG 2 cut(s) 431, 466
CviQI GTAC 9 cut(s) 346, 854, 862, 917, 937, 1099, 1184, 1669, 2162
DdeI CTNAG 6 cut(s) 215, 1064, 2169, 2550, 2786, 2925
DpnI GATC 5 cut(s) 86, 686, 1599, 1910, 2738
DpnII GATC 5 cut(s) 84, 684, 1597, 1908, 2736
DraIII CACNNNGTG 1 cut(s) 1330
EaeI YGGCCR 2 cut(s) 2562, 2580
EagI CGGCCG 1 cut(s) 2562
Eam1104I CTCTTC 6 cut(s) 251, 990, 2062, 2255, 2495, 2784
EarI CTCTTC 6 cut(s) 251, 990, 2062, 2255, 2495, 2784
EciI GGCGGA 1 cut(s) 1225
Ecl136II GAGCTC 2 cut(s) 1948, 2488
EclXI CGGCCG 1 cut(s) 2562
Eco130I CCWWGG 2 cut(s) 1287, 3249
Eco24I GRGCYC 2 cut(s) 1950, 2490
Eco31I GGTCTC 1 cut(s) 475
Eco32I GATATC 1 cut(s) 2191
Eco47I GGWCC 1 cut(s) 2378
Eco52I CGGCCG 1 cut(s) 2562
Eco53kI GAGCTC 2 cut(s) 1948, 2488
Eco57I CTGAAG 3 cut(s) 1700, 1821, 2501
Eco81I CCTNAGG 1 cut(s) 2169
Eco88I CYCGRG 2 cut(s) 1896, 2615
EcoICRI GAGCTC 2 cut(s) 1948, 2488
EcoNI CCTNNNNNAGG 1 cut(s) 1325
EcoRI GAATTC 3 cut(s) 154, 309, 1226
EcoRII CCWGG 1 cut(s) 1347
EcoRV GATATC 1 cut(s) 2191
EcoT14I CCWWGG 2 cut(s) 1287, 3249
EcoT38I GRGCYC 2 cut(s) 1950, 2490
ErhI CCWWGG 2 cut(s) 1287, 3249
FaqI GGGAC 1 cut(s) 1835
FauNDI CATATG 1 cut(s) 2217
FblI GTMKAC 1 cut(s) 1542
FokI GGATG 7 cut(s) 466, 1304, 1427, 1457, 2151, 2425, 2968
FriOI GRGCYC 2 cut(s) 1950, 2490
GlaI GCGC 2 cut(s) 2184, 2420
GsaI CCCAGC 1 cut(s) 717
GsuI CTGGAG 2 cut(s) 1316, 2340
HaeIII GGCC 6 cut(s) 875, 1081, 1094, 1894, 2564, 2582
HapII CCGG 5 cut(s) 1206, 1442, 2127, 2616, 3199
HgaI GACGC 3 cut(s) 238, 1326, 1888
HhaI GCGC 2 cut(s) 2185, 2421
Hin1I GRCGYC 2 cut(s) 249, 1337
Hin6I GCGC 2 cut(s) 2183, 2419
HinP1I GCGC 2 cut(s) 2183, 2419
HincII GTYRAC 4 cut(s) 357, 733, 1543, 2289
HindII GTYRAC 4 cut(s) 357, 733, 1543, 2289
HindIII AAGCTT 4 cut(s) 1765, 2510, 2905, 2913
HinfI GANTC 7 cut(s) 99, 625, 740, 1066, 1846, 2950, 3206
HpaI GTTAAC 2 cut(s) 733, 2289
HpaII CCGG 5 cut(s) 1206, 1442, 2127, 2616, 3199
HphI GGTGA 3 cut(s) 532, 1838, 2072
Hpy166II GTNNAC 8 cut(s) 26, 357, 733, 932, 1543, 1753, 2289, 2802
Hpy188I TCNGA 8 cut(s) 160, 795, 1272, 1530, 1801, 1945, 2941, 2955
Hpy8I GTNNAC 8 cut(s) 26, 357, 733, 932, 1543, 1753, 2289, 2802
Hpy99I CGWCG 6 cut(s) 1106, 1178, 1196, 1220, 1342, 2284
HpyAV CCTTC 7 cut(s) 299, 673, 1277, 1724, 1753, 2855, 3181
HpyF3I CTNAG 6 cut(s) 215, 1064, 2169, 2550, 2786, 2925
Hsp92I GRCGYC 2 cut(s) 249, 1337
HspAI GCGC 2 cut(s) 2183, 2419
KspAI GTTAAC 2 cut(s) 733, 2289
Kzo9I GATC 5 cut(s) 84, 684, 1597, 1908, 2736
LguI GCTCTTC 1 cut(s) 2495
LmnI GCTCC 4 cut(s) 1212, 1945, 2485, 2617
Lsp1109I GCAGC 9 cut(s) 135, 1526, 2035, 2320, 2403, 2582, 2609, 3022, 3038
LweI GCATC 8 cut(s) 824, 1405, 1441, 1722, 1767, 2335, 2403, 3073
MaeIII GTNAC 8 cut(s) 65, 137, 505, 538, 921, 1243, 1656, 2621
MalI GATC 5 cut(s) 86, 686, 1599, 1910, 2738
MbiI CCGCTC 1 cut(s) 2785
MboI GATC 5 cut(s) 84, 684, 1597, 1908, 2736
MfeI CAATTG 2 cut(s) 2390, 3210
MhlI GDGCHC 3 cut(s) 1578, 1950, 2490
MlsI TGGCCA 1 cut(s) 2582
MluNI TGGCCA 1 cut(s) 2582
MlyI GAGTC 2 cut(s) 93, 1075
MmeI TCCRAC 2 cut(s) 2390, 3169
Mox20I TGGCCA 1 cut(s) 2582
MroXI GAANNNNTTC 2 cut(s) 309, 744
MscI TGGCCA 1 cut(s) 2582
MslI CAYNNNNRTG 3 cut(s) 1087, 2471, 2530
Msp20I TGGCCA 1 cut(s) 2582
MspA1I CMGCKG 3 cut(s) 728, 2480, 3035
MspI CCGG 5 cut(s) 1206, 1442, 2127, 2616, 3199
MspR9I CCNGG 4 cut(s) 1207, 1349, 2616, 2617
MunI CAATTG 2 cut(s) 2390, 3210
Mva1269I GAATGC 2 cut(s) 267, 1288
MvaI CCWGG 1 cut(s) 1349
MvnI CGCG 1 cut(s) 2421
NciI CCSGG 3 cut(s) 1207, 2616, 2617
NdeI CATATG 1 cut(s) 2217
NdeII GATC 5 cut(s) 84, 684, 1597, 1908, 2736
NheI GCTAGC 3 cut(s) 1454, 1723, 3097
NmeAIII GCCGAG 1 cut(s) 2540
NmuCI GTSAC 5 cut(s) 65, 137, 538, 921, 1243
NspI RCATGY 6 cut(s) 914, 1606, 2507, 2990, 3026, 3059
NspV TTCGAA 2 cut(s) 152, 1874
PaeI GCATGC 1 cut(s) 2507
PaeR7I CTCGAG 1 cut(s) 1896
PciSI GCTCTTC 1 cut(s) 2495
PcsI WCGNNNNNNNCGW 3 cut(s) 149, 515, 2732
PctI GAATGC 2 cut(s) 267, 1288
PdmI GAANNNNTTC 2 cut(s) 309, 744
PfeI GAWTC 5 cut(s) 625, 740, 1846, 2950, 3206
PflFI GACNNNGTC 1 cut(s) 2690
PflMI CCANNNNNTGG 2 cut(s) 882, 1330
Ple19I CGATCG 1 cut(s) 1911
PleI GAGTC 2 cut(s) 93, 1074
PpsI GAGTC 2 cut(s) 93, 1074
PshAI GACNNNNGTC 1 cut(s) 135
PshBI ATTAAT 1 cut(s) 375
PsiI TTATAA 1 cut(s) 2094
Psp124BI GAGCTC 2 cut(s) 1950, 2490
Psp1406I AACGTT 1 cut(s) 1593
Psp6I CCWGG 1 cut(s) 1347
PspFI CCCAGC 1 cut(s) 713
PspGI CCWGG 1 cut(s) 1347
PspPI GGNCC 3 cut(s) 1079, 1092, 2378
PspXI VCTCGAGB 1 cut(s) 1896
PstI CTGCAG 1 cut(s) 2338
PsyI GACNNNGTC 1 cut(s) 2690
PvuI CGATCG 1 cut(s) 1911
PvuII CAGCTG 2 cut(s) 728, 3035
RsaI GTAC 9 cut(s) 347, 855, 863, 918, 938, 1100, 1185, 1670, 2163
RsaNI GTAC 9 cut(s) 346, 854, 862, 917, 937, 1099, 1184, 1669, 2162
RseI CAYNNNNRTG 3 cut(s) 1087, 2471, 2530
SacI GAGCTC 2 cut(s) 1950, 2490
SalI GTCGAC 1 cut(s) 1541
SapI GCTCTTC 1 cut(s) 2495
Sau3AI GATC 5 cut(s) 84, 684, 1597, 1908, 2736
Sau96I GGNCC 3 cut(s) 1079, 1092, 2378
ScaI AGTACT 1 cut(s) 918
SchI GAGTC 2 cut(s) 93, 1075
ScrFI CCNGG 4 cut(s) 1207, 1349, 2616, 2617
SduI GDGCHC 3 cut(s) 1578, 1950, 2490
SfaNI GCATC 8 cut(s) 824, 1405, 1441, 1722, 1767, 2335, 2403, 3073
SfcI CTRYAG 3 cut(s) 38, 1507, 2334
Sfr274I CTCGAG 1 cut(s) 1896
SfuI TTCGAA 2 cut(s) 152, 1874
SinI GGWCC 1 cut(s) 2378
SlaI CTCGAG 1 cut(s) 1896
SmaI CCCGGG 1 cut(s) 2617
SmiMI CAYNNNNRTG 3 cut(s) 1087, 2471, 2530
SmlI CTYRAG 4 cut(s) 300, 552, 1885, 1896
SmoI CTYRAG 4 cut(s) 300, 552, 1885, 1896
SphI GCATGC 1 cut(s) 2507
SspI AATATT 1 cut(s) 2133
SstI GAGCTC 2 cut(s) 1950, 2490
StyD4I CCNGG 4 cut(s) 1205, 1347, 2614, 2615
StyI CCWWGG 2 cut(s) 1287, 3249
TaqII GACCGA 1 cut(s) 84
TatI WGTACW 4 cut(s) 345, 861, 916, 1183
TauI GCSGC 3 cut(s) 1337, 1358, 2603
TfiI GAWTC 5 cut(s) 625, 740, 1846, 2950, 3206
TscAI CASTG 7 cut(s) 1068, 1186, 2112, 2311, 2551, 2590, 2614
TseFI GTSAC 5 cut(s) 65, 137, 538, 921, 1243
TseI GCWGC 9 cut(s) 123, 1514, 2048, 2333, 2416, 2570, 2597, 3026, 3035
Tsp45I GTSAC 5 cut(s) 65, 137, 538, 921, 1243
TspGWI ACGGA 4 cut(s) 1294, 1597, 2639, 2739
TspMI CCCGGG 1 cut(s) 2615
TspRI CASTG 7 cut(s) 1068, 1186, 2112, 2311, 2551, 2590, 2614
Tth111I GACNNNGTC 1 cut(s) 2690
Van91I CCANNNNNTGG 2 cut(s) 882, 1330
VpaK11BI GGWCC 1 cut(s) 2378
VspI ATTAAT 1 cut(s) 375
XagI CCTNNNNNAGG 1 cut(s) 1325
XapI RAATTY 8 cut(s) 115, 154, 309, 431, 463, 1226, 1273, 3139
XbaI TCTAGA 1 cut(s) 1460
XceI RCATGY 6 cut(s) 914, 1606, 2507, 2990, 3026, 3059
XcmI CCANNNNNNNNNTGG 1 cut(s) 3247
XhoI CTCGAG 1 cut(s) 1896
XmaI CCCGGG 1 cut(s) 2615
XmiI GTMKAC 1 cut(s) 1542
XmnI GAANNNNTTC 2 cut(s) 309, 744
ZrmI AGTACT 1 cut(s) 918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.