MD05G1026000.v1.1

Ankyrin repeat-containing protein At3g12360-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000724
Physical Location & Seq
Forward (+)
4180419 .. 4183223
2805 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1026000.v1.1.491

Sequence Viewer

Length: 1233 bp
ATGCGTGATGGTAAAACAAAGTTAACTATAATTTTAATTCCTATATATATGCATGATCAATCATACACAGGATGGCCTCGAGTCTTTTCGATCTTGAAGCAAATAAAAAAAGAAAAAACACTATCGGAGCTTACCCAGCTGCTCGTGGAGAAAGACTATTCGTGGGTAGAAAGTAAGAAACCAAAGAAAGACAAGACAATTTCCTTGGTGTCCTCACAGAAAGATATTGCTGCTATTTGTGGTGATGGAAGAGAAACAAAAAAATCTACTATTTATAACTACACCCCACTGCTTATAGCAACTAGCACCGGAATATTACCTCTTGTGAAAAGGATATATGAGGTACATCCTCAGGCGATTGAGGCGCACGAAATCGGCAGTCAACAAAACATTTTGCATATGTCGATTAAGCATCGTCAGTTGCAGATATTTAGGCTTGTGAAGAGAAGCAGAGCGATAACGTGGAGGTTAACTTCGAGGATTGACAGTGATGGAAATACCATATTGCATCGAGCTGCAGATATGACATATTACTCTCCAGACACTCAAAGTGTAAGTGGTCCTGCATTGCAATTGCAAGAAGAATTACGTTGGATGGTGCGAGCGGGAAAGATAACGCCACCTCATTATATCATGCACCGCAACAACAAGGGTGTGACAGCGGAGGAGCTCTTCAATATTGAGCATGAAAAGCTTCTTCATTCGGCACAAACATGGATAAAAGACACTGCTCAGTCATGCTCGACCGTGGCAGCTCTAGTGGCCACTGTGGTCTTTGCAGCCGCCTACACTGCTCCCGGGGGTTACAATTCAGGCGGTGTTCCTGTTCTCCAACATTCTCCTTTGTTCACTACCTTTATCATCACAGGCGTTGTCTCGCTCATCAGCTCATTGTCTTCTCTCGTCACATTTCTCTCGATCTTGACATCTCCGTTTGAGTACAAAAACTTCCATCATTCTCTTCCGCTCAGGCTGCATTTAGGATTTGCCTTTCTTTTCTTCTCGCTAGTGACGGCCATGCTCGCGTTCACTGCTACAGTTGTGCTCTTGATTCATCTCAACGAGAAATTGACATGGTCTCTCTTTTATGTTGCTGCGTTTCTTCCTGTGTCTGTATTTGGTATGCAGCTTCCTTTGTATGCGGGATTTTCTCATGCTTTGATATTTATATTTAAGAAAGTTAAGAAGTTTACAATTTCGTTTTTCCCTGTAAAGGAAATCCTCAATCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

411

Amino Acids

46.29

Weight (kDa)

9.65

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 238 - 348 4.2e-20 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 276
AccBSI CCGCTC 2 cut(s) 605, 967
AccII CGCG 1 cut(s) 1025
AciI CCGC 7 cut(s) 605, 640, 662, 783, 816, 965, 1142
AcoI YGGCCR 2 cut(s) 762, 1014
AfaI GTAC 2 cut(s) 345, 941
AfiI CCNNNNNNNGG 1 cut(s) 1213
AgsI TTSAA 2 cut(s) 97, 676
AluBI AGCT 8 cut(s) 130, 139, 515, 670, 694, 755, 888, 1129
AluI AGCT 8 cut(s) 130, 139, 515, 670, 694, 755, 888, 1129
Alw21I GWGCWC 2 cut(s) 672, 1047
Alw26I GTCTC 2 cut(s) 880, 1083
Ama87I CYCGRG 2 cut(s) 78, 797
AoxI GGCC 3 cut(s) 74, 762, 1014
ApeKI GCWGC 8 cut(s) 139, 230, 515, 752, 779, 973, 1094, 1126
Asp700I GAANNNNTTC 1 cut(s) 693
AspLEI GCGC 1 cut(s) 367
AspS9I GGNCC 1 cut(s) 560
AsuC2I CCSGG 2 cut(s) 798, 799
AsuHPI GGTGA 1 cut(s) 254
AvaI CYCGRG 2 cut(s) 78, 797
AvaII GGWCC 1 cut(s) 560
AxyI CCTNAGG 1 cut(s) 351
BalI TGGCCA 1 cut(s) 764
BanII GRGCYC 1 cut(s) 672
BarI GAAGNNNNNNTAC 2 cut(s) 932, 964
BauI CACGAG 1 cut(s) 143
BbsI GAAGAC 1 cut(s) 888
Bbv12I GWGCWC 2 cut(s) 672, 1047
BbvI GCAGC 8 cut(s) 126, 217, 502, 764, 791, 960, 1081, 1138
BccI CCATC 6 cut(s) 2, 66, 239, 485, 589, 960
BceAI ACGGC 1 cut(s) 1029
BclI TGATCA 1 cut(s) 55
BcnI CCSGG 2 cut(s) 798, 799
BcoDI GTCTC 2 cut(s) 880, 1083
BfaI CTAG 3 cut(s) 303, 758, 1007
BfmI CTRYAG 2 cut(s) 516, 1035
BisI GCNGC 9 cut(s) 140, 231, 516, 753, 780, 783, 974, 1095, 1127
BlsI GCNGC 9 cut(s) 141, 232, 517, 754, 781, 784, 975, 1096, 1128
Bme1390I CCNGG 2 cut(s) 798, 799
Bme18I GGWCC 1 cut(s) 560
BmeT110I CYCGRG 2 cut(s) 78, 797
BmgT120I GGNCC 1 cut(s) 560
BmrFI CCNGG 2 cut(s) 798, 799
BmsI GCATC 2 cut(s) 421, 517
BpiI GAAGAC 1 cut(s) 888
BpmI CTGGAG 1 cut(s) 522
Bpu10I CCTNAGC 1 cut(s) 968
BpuMI CCSGG 2 cut(s) 798, 799
BsaI GGTCTC 1 cut(s) 1083
BsaJI CCNNGG 4 cut(s) 204, 747, 797, 798
BsaWI WCCGGW 1 cut(s) 308
Bsc4I CCNNNNNNNGG 1 cut(s) 1213
Bse21I CCTNAGG 1 cut(s) 351
Bse3DI GCAATG 1 cut(s) 566
BseDI CCNNGG 4 cut(s) 204, 747, 797, 798
BseGI GGATG 3 cut(s) 77, 346, 600
BseLI CCNNNNNNNGG 1 cut(s) 1213
BseMI GCAATG 1 cut(s) 566
BseMII CTCAG 3 cut(s) 365, 746, 982
BseRI GAGGAG 1 cut(s) 680
BseXI GCAGC 8 cut(s) 126, 217, 502, 764, 791, 960, 1081, 1138
BseYI CCCAGC 1 cut(s) 135
Bsh1236I CGCG 1 cut(s) 1025
Bsh1285I CGRYCG 1 cut(s) 747
BshFI GGCC 3 cut(s) 76, 764, 1016
BsiEI CGRYCG 1 cut(s) 747
BsiHKAI GWGCWC 2 cut(s) 672, 1047
BsiHKCI CYCGRG 2 cut(s) 78, 797
BsiSI CCGG 2 cut(s) 309, 798
BslI CCNNNNNNNGG 1 cut(s) 1213
BsmAI GTCTC 2 cut(s) 880, 1083
BsnI GGCC 3 cut(s) 76, 764, 1016
Bso31I GGTCTC 1 cut(s) 1083
BsoBI CYCGRG 2 cut(s) 78, 797
Bsp1286I GDGCHC 2 cut(s) 672, 1047
Bsp143I GATC 3 cut(s) 55, 90, 918
BspACI CCGC 7 cut(s) 605, 640, 662, 783, 816, 965, 1142
BspANI GGCC 3 cut(s) 76, 764, 1016
BspCNI CTCAG 3 cut(s) 364, 745, 981
BspFNI CGCG 1 cut(s) 1025
BspMAI CTGCAG 1 cut(s) 520
BspQI GCTCTTC 1 cut(s) 677
BspTNI GGTCTC 1 cut(s) 1083
BsrBI CCGCTC 2 cut(s) 605, 967
BsrDI GCAATG 1 cut(s) 566
BssECI CCNNGG 4 cut(s) 204, 747, 797, 798
BssMI GATC 3 cut(s) 55, 90, 918
BssSI CACGAG 1 cut(s) 143
BssT1I CCWWGG 1 cut(s) 204
Bst2BI CACGAG 1 cut(s) 143
Bst4CI ACNGT 4 cut(s) 488, 748, 769, 1039
Bst6I CTCTTC 4 cut(s) 244, 437, 677, 966
BstC8I GCNNGC 2 cut(s) 603, 1023
BstDEI CTNAG 3 cut(s) 351, 732, 968
BstDSI CCRYGG 1 cut(s) 747
BstF5I GGATG 3 cut(s) 77, 346, 600
BstFNI CGCG 1 cut(s) 1025
BstHHI GCGC 1 cut(s) 367
BstKTI GATC 3 cut(s) 58, 93, 921
BstMAI GTCTC 2 cut(s) 880, 1083
BstMBI GATC 3 cut(s) 55, 90, 918
BstMCI CGRYCG 1 cut(s) 747
BstMWI GCNNNNNNNGC 8 cut(s) 136, 362, 691, 761, 791, 973, 1022, 1031
BstSCI CCNGG 2 cut(s) 796, 797
BstSFI CTRYAG 2 cut(s) 516, 1035
BstUI CGCG 1 cut(s) 1025
BstV1I GCAGC 8 cut(s) 126, 217, 502, 764, 791, 960, 1081, 1138
BstV2I GAAGAC 1 cut(s) 888
Bsu36I CCTNAGG 1 cut(s) 351
BsuRI GGCC 3 cut(s) 76, 764, 1016
BtgI CCRYGG 1 cut(s) 747
BtsCI GGATG 3 cut(s) 77, 346, 600
BtsI GCAGTG 4 cut(s) 287, 726, 789, 1029
BtsIMutI CAGTG 6 cut(s) 287, 493, 726, 765, 789, 1029
Cac8I GCNNGC 2 cut(s) 603, 1023
CfoI GCGC 1 cut(s) 367
Cfr13I GGNCC 1 cut(s) 560
Cfr9I CCCGGG 1 cut(s) 797
Csp6I GTAC 2 cut(s) 344, 940
CviAII CATG 8 cut(s) 53, 634, 686, 714, 738, 1018, 1074, 1154
CviQI GTAC 2 cut(s) 344, 940
DdeI CTNAG 3 cut(s) 351, 732, 968
DpnI GATC 3 cut(s) 57, 92, 920
DpnII GATC 3 cut(s) 55, 90, 918
EaeI YGGCCR 2 cut(s) 762, 1014
Eam1104I CTCTTC 4 cut(s) 244, 437, 677, 966
EarI CTCTTC 4 cut(s) 244, 437, 677, 966
Ecl136II GAGCTC 1 cut(s) 670
Eco130I CCWWGG 1 cut(s) 204
Eco24I GRGCYC 1 cut(s) 672
Eco31I GGTCTC 1 cut(s) 1083
Eco47I GGWCC 1 cut(s) 560
Eco53kI GAGCTC 1 cut(s) 670
Eco81I CCTNAGG 1 cut(s) 351
Eco88I CYCGRG 2 cut(s) 78, 797
EcoICRI GAGCTC 1 cut(s) 670
EcoT14I CCWWGG 1 cut(s) 204
EcoT22I ATGCAT 1 cut(s) 54
EcoT38I GRGCYC 1 cut(s) 672
ErhI CCWWGG 1 cut(s) 204
FaeI CATG 8 cut(s) 56, 637, 689, 717, 741, 1021, 1077, 1157
FatI CATG 8 cut(s) 52, 633, 685, 713, 737, 1017, 1073, 1153
FauI CCCGC 2 cut(s) 598, 1135
FauNDI CATATG 1 cut(s) 399
FbaI TGATCA 1 cut(s) 55
Fnu4HI GCNGC 9 cut(s) 140, 231, 516, 753, 780, 783, 974, 1095, 1127
FokI GGATG 3 cut(s) 84, 333, 607
FriOI GRGCYC 1 cut(s) 672
Fsp4HI GCNGC 9 cut(s) 140, 231, 516, 753, 780, 783, 974, 1095, 1127
FspBI CTAG 3 cut(s) 303, 758, 1007
GlaI GCGC 1 cut(s) 366
GluI GCNGC 9 cut(s) 140, 231, 516, 753, 780, 783, 974, 1095, 1127
GsaI CCCAGC 1 cut(s) 139
GsuI CTGGAG 1 cut(s) 522
HaeIII GGCC 3 cut(s) 76, 764, 1016
HapII CCGG 2 cut(s) 309, 798
HhaI GCGC 1 cut(s) 367
Hin1II CATG 8 cut(s) 56, 637, 689, 717, 741, 1021, 1077, 1157
Hin6I GCGC 1 cut(s) 365
HinP1I GCGC 1 cut(s) 365
HincII GTYRAC 3 cut(s) 24, 383, 471
HindII GTYRAC 3 cut(s) 24, 383, 471
HindIII AAGCTT 1 cut(s) 692
HinfI GANTC 2 cut(s) 81, 1051
HpaI GTTAAC 2 cut(s) 24, 471
HpaII CCGG 2 cut(s) 309, 798
HphI GGTGA 1 cut(s) 254
Hpy166II GTNNAC 6 cut(s) 24, 383, 471, 849, 1029, 1191
Hpy188I TCNGA 1 cut(s) 127
Hpy188III TCNNGA 5 cut(s) 94, 539, 916, 922, 1048
Hpy8I GTNNAC 6 cut(s) 24, 383, 471, 849, 1029, 1191
HpyCH4III ACNGT 4 cut(s) 488, 748, 769, 1039
HpyCH4IV ACGT 2 cut(s) 461, 589
HpyF10VI GCNNNNNNNGC 8 cut(s) 136, 362, 691, 761, 791, 973, 1022, 1031
HpyF3I CTNAG 3 cut(s) 351, 732, 968
HpySE526I ACGT 2 cut(s) 461, 589
Hsp92II CATG 8 cut(s) 56, 637, 689, 717, 741, 1021, 1077, 1157
HspAI GCGC 1 cut(s) 365
Ksp22I TGATCA 1 cut(s) 55
KspAI GTTAAC 2 cut(s) 24, 471
Kzo9I GATC 3 cut(s) 55, 90, 918
LguI GCTCTTC 1 cut(s) 677
LmnI GCTCC 3 cut(s) 127, 667, 799
Lsp1109I GCAGC 8 cut(s) 126, 217, 502, 764, 791, 960, 1081, 1138
LweI GCATC 2 cut(s) 421, 517
MaeI CTAG 3 cut(s) 303, 758, 1007
MaeII ACGT 2 cut(s) 461, 589
MaeIII GTNAC 4 cut(s) 655, 803, 904, 1009
MalI GATC 3 cut(s) 57, 92, 920
MbiI CCGCTC 2 cut(s) 605, 967
MboI GATC 3 cut(s) 55, 90, 918
MboII GAAGA 9 cut(s) 261, 454, 593, 664, 689, 888, 953, 991, 1094
MfeI CAATTG 1 cut(s) 572
MhlI GDGCHC 2 cut(s) 672, 1047
MlsI TGGCCA 1 cut(s) 764
MluCI AATT 8 cut(s) 30, 36, 198, 572, 584, 808, 1067, 1194
MluNI TGGCCA 1 cut(s) 764
MlyI GAGTC 1 cut(s) 90
MmeI TCCRAC 2 cut(s) 572, 856
Mox20I TGGCCA 1 cut(s) 764
Mph1103I ATGCAT 1 cut(s) 54
MroXI GAANNNNTTC 1 cut(s) 693
MscI TGGCCA 1 cut(s) 764
MseI TTAA 6 cut(s) 23, 35, 408, 470, 1173, 1182
MslI CAYNNNNRTG 1 cut(s) 712
Msp20I TGGCCA 1 cut(s) 764
MspA1I CMGCKG 2 cut(s) 139, 662
MspI CCGG 2 cut(s) 309, 798
MspR9I CCNGG 2 cut(s) 798, 799
MunI CAATTG 1 cut(s) 572
MvnI CGCG 1 cut(s) 1025
MwoI GCNNNNNNNGC 8 cut(s) 136, 362, 691, 761, 791, 973, 1022, 1031
NciI CCSGG 2 cut(s) 798, 799
NdeI CATATG 1 cut(s) 399
NdeII GATC 3 cut(s) 55, 90, 918
NlaIII CATG 8 cut(s) 56, 637, 689, 717, 741, 1021, 1077, 1157
NmuCI GTSAC 3 cut(s) 655, 904, 1009
NsiI ATGCAT 1 cut(s) 54
PaeR7I CTCGAG 1 cut(s) 78
PciSI GCTCTTC 1 cut(s) 677
PdmI GAANNNNTTC 1 cut(s) 693
PfeI GAWTC 1 cut(s) 1051
PflFI GACNNNGTC 1 cut(s) 1075
PkrI GCNGC 9 cut(s) 141, 232, 517, 754, 781, 784, 975, 1096, 1128
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
PsiI TTATAA 1 cut(s) 276
Psp124BI GAGCTC 1 cut(s) 672
PspFI CCCAGC 1 cut(s) 135
PspPI GGNCC 1 cut(s) 560
PspXI VCTCGAGB 1 cut(s) 78
PstI CTGCAG 1 cut(s) 520
PsyI GACNNNGTC 1 cut(s) 1075
PvuII CAGCTG 1 cut(s) 139
RsaI GTAC 2 cut(s) 345, 941
RsaNI GTAC 2 cut(s) 344, 940
RseI CAYNNNNRTG 1 cut(s) 712
SacI GAGCTC 1 cut(s) 672
SapI GCTCTTC 1 cut(s) 677
SaqAI TTAA 6 cut(s) 23, 35, 408, 470, 1173, 1182
SatI GCNGC 9 cut(s) 140, 231, 516, 753, 780, 783, 974, 1095, 1127
Sau3AI GATC 3 cut(s) 55, 90, 918
Sau96I GGNCC 1 cut(s) 560
SchI GAGTC 1 cut(s) 90
ScrFI CCNGG 2 cut(s) 798, 799
SduI GDGCHC 2 cut(s) 672, 1047
SfaNI GCATC 2 cut(s) 421, 517
SfcI CTRYAG 2 cut(s) 516, 1035
Sfr274I CTCGAG 1 cut(s) 78
SinI GGWCC 1 cut(s) 560
SlaI CTCGAG 1 cut(s) 78
SmaI CCCGGG 1 cut(s) 799
SmiMI CAYNNNNRTG 1 cut(s) 712
SmlI CTYRAG 1 cut(s) 78
SmoI CTYRAG 1 cut(s) 78
Sse9I AATT 8 cut(s) 30, 36, 198, 572, 584, 808, 1067, 1194
SsiI CCGC 7 cut(s) 605, 640, 662, 783, 816, 965, 1142
SspI AATATT 2 cut(s) 315, 679
SspMI CTAG 3 cut(s) 303, 758, 1007
SstI GAGCTC 1 cut(s) 672
StyD4I CCNGG 2 cut(s) 796, 797
StyI CCWWGG 1 cut(s) 204
TaaI ACNGT 4 cut(s) 488, 748, 769, 1039
TaiI ACGT 2 cut(s) 464, 592
TaqI TCGA 7 cut(s) 79, 89, 404, 476, 511, 743, 917
TasI AATT 8 cut(s) 30, 36, 198, 572, 584, 808, 1067, 1194
TatI WGTACW 1 cut(s) 939
TauI GCSGC 1 cut(s) 785
TfiI GAWTC 1 cut(s) 1051
Tru1I TTAA 6 cut(s) 23, 35, 408, 470, 1173, 1182
Tru9I TTAA 6 cut(s) 23, 35, 408, 470, 1173, 1182
TscAI CASTG 6 cut(s) 294, 493, 733, 772, 796, 1036
TseFI GTSAC 3 cut(s) 655, 904, 1009
TseI GCWGC 8 cut(s) 139, 230, 515, 752, 779, 973, 1094, 1126
Tsp45I GTSAC 3 cut(s) 655, 904, 1009
TspDTI ATGAA 3 cut(s) 689, 702, 1043
TspGWI ACGGA 1 cut(s) 921
TspMI CCCGGG 1 cut(s) 797
TspRI CASTG 6 cut(s) 294, 493, 733, 772, 796, 1036
Tth111I GACNNNGTC 1 cut(s) 1075
VpaK11BI GGWCC 1 cut(s) 560
XhoI CTCGAG 1 cut(s) 78
XmaI CCCGGG 1 cut(s) 797
XmnI GAANNNNTTC 1 cut(s) 693
XspI CTAG 3 cut(s) 303, 758, 1007
Zsp2I ATGCAT 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.