MD05G1353500.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000724
Physical Location & Seq
Reverse (-)
46975928 .. 46980751
4824 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1353500.v1.1.491

Sequence Viewer

Length: 2340 bp
ATGAACCTGGACGGGGACAATGTACTTCACCTTGCGGGTTCTTCTTGCAGCAAATCACAATGCAAAGGAGTCCTCCAAAATTTGATTGATTTATGCAGCGATTCAACCACCATGGCCAAGAGTTTGAGGGCTCCGAATATAGACGGAAACAATGCTCTTCACCAGGTGAGTGTGTCCGGCAGTGTGGAAGCAGCTAAGTTCTTGGTGAAGAAGTTCAACGATCCTGTGGTTCGTACTGATGAAGAGAATAATGATGCACTGGAGCTGCTATGGACTCGGAACCATTTAGGAGAAACTCCGCTCTACAGAGCGGCTACTCTCGGTCACACTGACTTGGTCAAATTTTACTTGGAAACACTGATGCTGAAATGCAAGGAGAAGTATCAGGGAATGTCTTGGGAAATGTATCGGCAAGACTGTTGGCCGCAGCTCATTAGGCCTGACGGAATGTCCGTTCTTCGTATGGCTGTCGTCGGCCAACATTTTGAGACTGCTCATTGGTTGCTAAAGTTCTACCCTTTTCTAGGACAAATGAAAGACGAAAATGGATTGACAAGCCTTCAATTGTTAGCCCAAATGCCAACTGCCTCTGAGCCACATTGTCAACAAAGCCAATGGAAGATGCTCATTTATCATTGCCTTCGTGTTAGAGAAGATGACGTGGAGAGCGGCATGGATAGCACCCATCTCCGTCAATCCATGGATAGCACCCATCATCAGGATGACGTGGAGAGCGGCACGGATACCAACCATCCCCGTCAATCCATCTTTCGGAAGAAACTTGCAGTTGTTATGAAGGCTTATTATTCATTATGGGACTCCCTTGCTAAAGAATCAGAAGTCCACGACGGCATAATCTACAGAATATGGAAGGACAAGAAAAATAAAAAATCACTAACGAAACTCATCAAATTGCTCGTCAACTCGGACCATACTTGGTTGATTACTGACAATCAAGAAAACAAGAGGACCATTTCTCTGGGGCCATTTAAAGATGGTGATGACGGAGGCGAAAAAGGAACCCAATCGAACAAGACAATTGATAGTGATGGAGGTGATAAACAAAAGGACGCAACCAAATTGAAAAAGGAGGCGAGGAAGGAAGTTTACAAGTCTACCCCATTGCTTGACAAGATAATTGATAGTGATGGAGGTGATGAACAAAAGGAAGCAAACAAATTGAAAAATATCACAGGAAATGTACCCATTGTGCAGGAGATACTAGAGCAGCATCCTCAAGCAGTCAAGTATGTTAACGAAGAGGGAGTGGCTTGGTTGCTTGTGCTAGCAAATGCTGATGGAGGTGATAAACAAAAGGAAGCAACCAAATTGAAAAAGGAGGCGAGGAAGGAAGTTTACACGTCTACCCCATTGCTTATAGCATCTGTCACAGGAAATGTACACATTGTGAAGGAGATACTTGAGCAGCATCCTCAAGCAGTCGAGCATGTTAACGAAAAGGAACACAACATTTTGCACCTGGCCATTAAGAACCGTCAGGAAAAGATCTTTGATCTCATCAAAAGCAACCTAACTGTGATGTCGAAGCTGAGTAAGAGGATAGACAGCAATGGAAACACCATATTGCACCAGGCTGCAGATAGGAGCTACTACTCTGTATCATTGTCTAACAAATTAATAGGCCCTGCCATGCAATTACAAGATGAGCTACGCTGGTTCCAGCGTGTAAAAGAGATCGTACCGCCTCATTACATCATTCACCACAACAAGAAGGATAAGACAGCGGAGGAGCTGTTCAACGATTGGCATGACCAACTTCTGGAGGAAGCACAAAAATGGGTAAAAGAAACGGCTCAGTCATGCTCAACGGTGGCGGTGCTAGTGGCTACTGTAGTCTTTGCAGCTGCCTACACCATTCCTGGGGGTACTAATGACCAAAATGGCCTTCCTCTTTTCCAGAACGATCCTCTCTTTATGCTCTTCACTAGCATGGATGTTGTGGCCATCGCCAGCTCATTATCTTCTGTGGCATTCTTTCTCTCGGTCCTCTCCTCCCCTCTCGAGTATCCACTTTTCGTTAGCAGCATTCCTAACAAGGTCATGGCAGGATTCATCTTTCTCTTCATCTCCATGGCAACAACTATGCTCGCCTTTGCTGCCACCATTTTGTTGTTGATTCGCGTCGAGAAGAAATGGACCAAGTCTTTACTTTACCCTATTGCCTTTTTCCCAGTCCCTCTATTCGGCCTGCTTCAGTTTCCTATGTACCAATCCTTTCGAGTCATGTTTCATGAAGTTAATGCCTGGTTTTTCAAACCCGTACTCTACTTTCTTAGCCTTAGGAGATCACTATGGTGCAAGAATAAGAAGCATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

780

Amino Acids

88.5

Weight (kDa)

7.38

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 24 - 116 2.8e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 414 - 507 7.1e-06 Ankyrin repeats (3 copies)
PGG PF13962 599 - 710 1.3e-20 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1780
AccBSI CCGCTC 4 cut(s) 301, 311, 669, 735
AccI GTMKAC 2 cut(s) 1115, 1364
AccII CGCG 1 cut(s) 2142
AciI CCGC 9 cut(s) 35, 299, 311, 425, 669, 735, 1703, 1745, 1835
AclWI GGATC 2 cut(s) 215, 1919
AcoI YGGCCR 5 cut(s) 114, 422, 475, 1482, 1962
AcsI RAATTY 2 cut(s) 79, 341
AcuI CTGAAG 1 cut(s) 2198
AdeI CACNNNGTG 2 cut(s) 166, 1408
AfaI GTAC 8 cut(s) 24, 235, 1203, 1401, 1701, 1888, 2228, 2283
AfiI CCNNNNNNNGG 7 cut(s) 13, 524, 718, 771, 1780, 1881, 2204
AflIII ACRYGT 1 cut(s) 1359
AgsI TTSAA 8 cut(s) 105, 217, 563, 1084, 1183, 1333, 1759, 2275
AhdI GACNNNNNGTC 1 cut(s) 448
AjiI CACGTC 3 cut(s) 661, 727, 1362
AjnI CCWGG 6 cut(s) 6, 162, 1479, 1590, 1879, 2264
AjuI GAANNNNNNNTTGG 2 cut(s) 1890, 1922
AleI CACNNNNGTG 1 cut(s) 2314
AloI GAACNNNNNNTCC 4 cut(s) 438, 470, 1739, 1771
AluBI AGCT 9 cut(s) 194, 265, 430, 1549, 1608, 1669, 1753, 1865, 1974
AluI AGCT 9 cut(s) 194, 265, 430, 1549, 1608, 1669, 1753, 1865, 1974
Alw26I GTCTC 1 cut(s) 482
AlwI GGATC 2 cut(s) 215, 1919
Ama87I CYCGRG 1 cut(s) 2021
ApoI RAATTY 2 cut(s) 79, 341
ArsI GACNNNNNNTTYG 2 cut(s) 903, 935
AseI ATTAAT 1 cut(s) 1637
Asp700I GAANNNNTTC 1 cut(s) 212
AspS9I GGNCC 6 cut(s) 928, 969, 983, 1643, 2005, 2157
AsuHPI GGTGA 9 cut(s) 20, 152, 178, 217, 1010, 1067, 1166, 1316, 1712
AsuNHI GCTAGC 1 cut(s) 1285
AvaI CYCGRG 1 cut(s) 2021
AvaII GGWCC 4 cut(s) 928, 969, 2005, 2157
AxyI CCTNAGG 1 cut(s) 2300
BalI TGGCCA 3 cut(s) 116, 1484, 1964
BanII GRGCYC 1 cut(s) 133
BarI GAAGNNNNNNTAC 4 cut(s) 1091, 1123, 1340, 1372
BccI CCATC 9 cut(s) 693, 720, 759, 773, 989, 1043, 1142, 1292, 1973
BceAI ACGGC 2 cut(s) 865, 1827
BciT130I CCWGG 6 cut(s) 8, 164, 1481, 1592, 1881, 2266
BciVI GTATCC 2 cut(s) 736, 2037
BcoDI GTCTC 1 cut(s) 482
BfaI CTAG 5 cut(s) 524, 1223, 1286, 1841, 1947
BfmI CTRYAG 4 cut(s) 304, 859, 1596, 1851
BfuI GTATCC 2 cut(s) 736, 2037
BglII AGATCT 1 cut(s) 1506
Bme1390I CCNGG 6 cut(s) 8, 164, 1481, 1592, 1881, 2266
Bme18I GGWCC 4 cut(s) 928, 969, 2005, 2157
BmeRI GACNNNNNGTC 1 cut(s) 448
BmeT110I CYCGRG 1 cut(s) 2021
BmgBI CACGTC 3 cut(s) 661, 727, 1362
BmgT120I GGNCC 6 cut(s) 928, 969, 983, 1643, 2005, 2157
BmiI GGNNCC 5 cut(s) 132, 281, 984, 1021, 1679
BmrFI CCNGG 6 cut(s) 8, 164, 1481, 1592, 1881, 2266
BmrI ACTGGG 1 cut(s) 2186
BmsI GCATC 6 cut(s) 244, 351, 612, 1240, 1391, 1438
BmtI GCTAGC 1 cut(s) 1289
BmuI ACTGGG 1 cut(s) 2186
BpmI CTGGAG 2 cut(s) 281, 1802
BpuEI CTTGAG 3 cut(s) 1221, 1419, 1442
BsaJI CCNNGG 4 cut(s) 111, 699, 1880, 2091
BsaXI ACNNNNNCTCC 2 cut(s) 1739, 1769
Bsc4I CCNNNNNNNGG 7 cut(s) 13, 524, 718, 771, 1780, 1881, 2204
Bse1I ACTGG 2 cut(s) 264, 2192
Bse21I CCTNAGG 1 cut(s) 2300
Bse3DI GCAATG 4 cut(s) 634, 1121, 1370, 1576
BseBI CCWGG 6 cut(s) 8, 164, 1481, 1592, 1881, 2266
BseDI CCNNGG 4 cut(s) 111, 699, 1880, 2091
BseGI GGATG 5 cut(s) 727, 751, 1231, 1429, 1960
BseLI CCNNNNNNNGG 7 cut(s) 13, 524, 718, 771, 1780, 1881, 2204
BseMI GCAATG 4 cut(s) 634, 1121, 1370, 1576
BseMII CTCAG 3 cut(s) 582, 1541, 1829
BseNI ACTGG 2 cut(s) 264, 2192
BseRI GAGGAG 2 cut(s) 1763, 2002
BsgI GTGCAG 1 cut(s) 1232
Bsh1236I CGCG 1 cut(s) 2142
BsiHKCI CYCGRG 1 cut(s) 2021
BsiSI CCGG 1 cut(s) 177
BslFI GGGAC 3 cut(s) 29, 830, 2180
BslI CCNNNNNNNGG 7 cut(s) 13, 524, 718, 771, 1780, 1881, 2204
BsmAI GTCTC 1 cut(s) 482
BsmFI GGGAC 3 cut(s) 29, 830, 2180
BsmI GAATGC 2 cut(s) 1991, 2046
BsoBI CYCGRG 1 cut(s) 2021
Bsp1286I GDGCHC 1 cut(s) 133
Bsp1407I TGTACA 1 cut(s) 1399
Bsp143I GATC 6 cut(s) 220, 1506, 1513, 1695, 1924, 2306
Bsp19I CCATGG 3 cut(s) 111, 699, 2091
BspACI CCGC 9 cut(s) 35, 299, 311, 425, 669, 735, 1703, 1745, 1835
BspCNI CTCAG 3 cut(s) 583, 1542, 1828
BspFNI CGCG 1 cut(s) 2142
BspHI TCATGA 1 cut(s) 2251
BspLI GGNNCC 5 cut(s) 132, 281, 984, 1021, 1679
BspMAI CTGCAG 1 cut(s) 1600
BspOI GCTAGC 1 cut(s) 1289
BspPI GGATC 2 cut(s) 215, 1919
BspQI GCTCTTC 2 cut(s) 162, 1946
BsrBI CCGCTC 4 cut(s) 301, 311, 669, 735
BsrDI GCAATG 4 cut(s) 634, 1121, 1370, 1576
BsrGI TGTACA 1 cut(s) 1399
BsrI ACTGG 2 cut(s) 264, 2192
BssECI CCNNGG 4 cut(s) 111, 699, 1880, 2091
BssMI GATC 6 cut(s) 220, 1506, 1513, 1695, 1924, 2306
BssT1I CCWWGG 3 cut(s) 111, 699, 2091
Bst2UI CCWGG 6 cut(s) 8, 164, 1481, 1592, 1881, 2266
Bst4CI ACNGT 5 cut(s) 419, 1496, 1537, 1831, 1852
Bst6I CTCTTC 5 cut(s) 162, 237, 1254, 1946, 2087
BstAUI TGTACA 1 cut(s) 1399
BstC8I GCNNGC 4 cut(s) 1287, 1972, 2109, 2210
BstDEI CTNAG 6 cut(s) 195, 591, 1550, 1815, 2294, 2300
BstDSI CCRYGG 3 cut(s) 111, 699, 2091
BstENI CCTNNNNNAGG 1 cut(s) 522
BstF5I GGATG 5 cut(s) 727, 751, 1231, 1429, 1960
BstFNI CGCG 1 cut(s) 2142
BstKTI GATC 6 cut(s) 223, 1509, 1516, 1698, 1927, 2309
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 6 cut(s) 220, 1506, 1513, 1695, 1924, 2306
BstMWI GCNNNNNNNGC 3 cut(s) 436, 678, 2117
BstNI CCWGG 6 cut(s) 8, 164, 1481, 1592, 1881, 2266
BstNSI RCATGY 1 cut(s) 1451
BstSCI CCNGG 6 cut(s) 6, 162, 1479, 1590, 1879, 2264
BstSFI CTRYAG 4 cut(s) 304, 859, 1596, 1851
BstUI CGCG 1 cut(s) 2142
BstX2I RGATCY 1 cut(s) 1506
BstXI CCANNNNNNTGG 1 cut(s) 979
BstYI RGATCY 1 cut(s) 1506
Bsu36I CCTNAGG 1 cut(s) 2300
BsuI GTATCC 2 cut(s) 736, 2037
BtgI CCRYGG 3 cut(s) 111, 699, 2091
BtgZI GCGATG 1 cut(s) 1951
BtrI CACGTC 3 cut(s) 661, 727, 1362
BtsCI GGATG 5 cut(s) 727, 751, 1231, 1429, 1960
BtsI GCAGTG 1 cut(s) 187
BtsIMutI CAGTG 4 cut(s) 187, 257, 327, 356
Cac8I GCNNGC 4 cut(s) 1287, 1972, 2109, 2210
CciI TCATGA 1 cut(s) 2251
Cfr13I GGNCC 6 cut(s) 928, 969, 983, 1643, 2005, 2157
CseI GACGC 2 cut(s) 1079, 2131
CsiI ACCWGGT 1 cut(s) 162
Csp6I GTAC 8 cut(s) 23, 234, 1202, 1400, 1700, 1887, 2227, 2282
CviQI GTAC 8 cut(s) 23, 234, 1202, 1400, 1700, 1887, 2227, 2282
DdeI CTNAG 6 cut(s) 195, 591, 1550, 1815, 2294, 2300
DpnI GATC 6 cut(s) 222, 1508, 1515, 1697, 1926, 2308
DpnII GATC 6 cut(s) 220, 1506, 1513, 1695, 1924, 2306
DraI TTTAAA 1 cut(s) 991
DraIII CACNNNGTG 2 cut(s) 166, 1408
DriI GACNNNNNGTC 1 cut(s) 448
EaeI YGGCCR 5 cut(s) 114, 422, 475, 1482, 1962
Eam1104I CTCTTC 5 cut(s) 162, 237, 1254, 1946, 2087
Eam1105I GACNNNNNGTC 1 cut(s) 448
EarI CTCTTC 5 cut(s) 162, 237, 1254, 1946, 2087
Eco130I CCWWGG 3 cut(s) 111, 699, 2091
Eco147I AGGCCT 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 133
Eco47I GGWCC 4 cut(s) 928, 969, 2005, 2157
Eco57I CTGAAG 1 cut(s) 2198
Eco81I CCTNAGG 1 cut(s) 2300
Eco88I CYCGRG 1 cut(s) 2021
EcoNI CCTNNNNNAGG 1 cut(s) 522
EcoO109I RGGNCCY 1 cut(s) 1643
EcoRII CCWGG 6 cut(s) 6, 162, 1479, 1590, 1879, 2264
EcoT14I CCWWGG 3 cut(s) 111, 699, 2091
EcoT38I GRGCYC 1 cut(s) 133
ErhI CCWWGG 3 cut(s) 111, 699, 2091
FaqI GGGAC 3 cut(s) 29, 830, 2180
FauI CCCGC 1 cut(s) 28
FblI GTMKAC 2 cut(s) 1115, 1364
FokI GGATG 5 cut(s) 734, 738, 1218, 1416, 1967
FriOI GRGCYC 1 cut(s) 133
FspBI CTAG 5 cut(s) 524, 1223, 1286, 1841, 1947
GsuI CTGGAG 2 cut(s) 281, 1802
HapII CCGG 1 cut(s) 177
HgaI GACGC 2 cut(s) 1079, 2131
HincII GTYRAC 4 cut(s) 605, 922, 1255, 1453
HindII GTYRAC 4 cut(s) 605, 922, 1255, 1453
HinfI GANTC 8 cut(s) 69, 101, 274, 818, 833, 2070, 2137, 2241
HpaI GTTAAC 2 cut(s) 1255, 1453
HpaII CCGG 1 cut(s) 177
HphI GGTGA 9 cut(s) 20, 152, 178, 217, 1010, 1067, 1166, 1316, 1712
Hpy188I TCNGA 6 cut(s) 135, 279, 592, 774, 838, 928
Hpy188III TCNNGA 8 cut(s) 719, 956, 1499, 1781, 1918, 2021, 2146, 2252
Hpy99I CGWCG 3 cut(s) 476, 851, 2147
HpyAV CCTTC 9 cut(s) 569, 650, 790, 865, 1093, 1342, 1405, 1726, 1916
HpyCH4III ACNGT 5 cut(s) 419, 1496, 1537, 1831, 1852
HpyCH4IV ACGT 3 cut(s) 660, 726, 1361
HpyF10VI GCNNNNNNNGC 3 cut(s) 436, 678, 2117
HpyF3I CTNAG 6 cut(s) 195, 591, 1550, 1815, 2294, 2300
HpySE526I ACGT 3 cut(s) 660, 726, 1361
KspAI GTTAAC 2 cut(s) 1255, 1453
Kzo9I GATC 6 cut(s) 220, 1506, 1513, 1695, 1924, 2306
LguI GCTCTTC 2 cut(s) 162, 1946
LmnI GCTCC 4 cut(s) 136, 262, 1605, 1750
LweI GCATC 6 cut(s) 244, 351, 612, 1240, 1391, 1438
MabI ACCWGGT 1 cut(s) 162
MaeI CTAG 5 cut(s) 524, 1223, 1286, 1841, 1947
MaeII ACGT 3 cut(s) 660, 726, 1361
MaeIII GTNAC 2 cut(s) 323, 1387
MalI GATC 6 cut(s) 222, 1508, 1515, 1697, 1926, 2308
MbiI CCGCTC 4 cut(s) 301, 311, 669, 735
MboI GATC 6 cut(s) 220, 1506, 1513, 1695, 1924, 2306
MfeI CAATTG 2 cut(s) 563, 1038
MflI RGATCY 1 cut(s) 1506
MhlI GDGCHC 1 cut(s) 133
MlsI TGGCCA 3 cut(s) 116, 1484, 1964
MluNI TGGCCA 3 cut(s) 116, 1484, 1964
MlyI GAGTC 4 cut(s) 78, 268, 812, 2250
Mox20I TGGCCA 3 cut(s) 116, 1484, 1964
MroXI GAANNNNTTC 1 cut(s) 212
MscI TGGCCA 3 cut(s) 116, 1484, 1964
MseI TTAA 6 cut(s) 990, 1254, 1452, 1488, 1637, 2259
MslI CAYNNNNRTG 5 cut(s) 720, 1795, 1949, 2090, 2314
Msp20I TGGCCA 3 cut(s) 116, 1484, 1964
MspA1I CMGCKG 2 cut(s) 1745, 1865
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 6 cut(s) 8, 164, 1481, 1592, 1881, 2266
MunI CAATTG 2 cut(s) 563, 1038
Mva1269I GAATGC 2 cut(s) 1991, 2046
MvaI CCWGG 6 cut(s) 8, 164, 1481, 1592, 1881, 2266
MvnI CGCG 1 cut(s) 2142
MwoI GCNNNNNNNGC 3 cut(s) 436, 678, 2117
NcoI CCATGG 3 cut(s) 111, 699, 2091
NdeII GATC 6 cut(s) 220, 1506, 1513, 1695, 1924, 2306
NheI GCTAGC 1 cut(s) 1285
NlaIV GGNNCC 5 cut(s) 132, 281, 984, 1021, 1679
NmuCI GTSAC 2 cut(s) 323, 1387
NspI RCATGY 1 cut(s) 1451
OliI CACNNNNGTG 1 cut(s) 2314
PaeR7I CTCGAG 1 cut(s) 2021
PagI TCATGA 1 cut(s) 2251
PceI AGGCCT 1 cut(s) 439
PciSI GCTCTTC 2 cut(s) 162, 1946
PcsI WCGNNNNNNNCGW 1 cut(s) 450
PctI GAATGC 2 cut(s) 1991, 2046
PdmI GAANNNNTTC 1 cut(s) 212
PfeI GAWTC 4 cut(s) 101, 833, 2070, 2137
PflFI GACNNNGTC 2 cut(s) 335, 2161
PflMI CCANNNNNTGG 1 cut(s) 1780
PleI GAGTC 4 cut(s) 77, 268, 812, 2249
PpsI GAGTC 4 cut(s) 77, 268, 812, 2249
PshBI ATTAAT 1 cut(s) 1637
Psp6I CCWGG 6 cut(s) 6, 162, 1479, 1590, 1879, 2264
PspGI CCWGG 6 cut(s) 6, 162, 1479, 1590, 1879, 2264
PspN4I GGNNCC 5 cut(s) 132, 281, 984, 1021, 1679
PspPI GGNCC 6 cut(s) 928, 969, 983, 1643, 2005, 2157
PstI CTGCAG 1 cut(s) 1600
PsuI RGATCY 1 cut(s) 1506
PsyI GACNNNGTC 2 cut(s) 335, 2161
PvuII CAGCTG 1 cut(s) 1865
RsaI GTAC 8 cut(s) 24, 235, 1203, 1401, 1701, 1888, 2228, 2283
RsaNI GTAC 8 cut(s) 23, 234, 1202, 1400, 1700, 1887, 2227, 2282
RseI CAYNNNNRTG 5 cut(s) 720, 1795, 1949, 2090, 2314
SapI GCTCTTC 2 cut(s) 162, 1946
SaqAI TTAA 6 cut(s) 990, 1254, 1452, 1488, 1637, 2259
Sau3AI GATC 6 cut(s) 220, 1506, 1513, 1695, 1924, 2306
Sau96I GGNCC 6 cut(s) 928, 969, 983, 1643, 2005, 2157
SchI GAGTC 4 cut(s) 78, 268, 812, 2250
ScrFI CCNGG 6 cut(s) 8, 164, 1481, 1592, 1881, 2266
SduI GDGCHC 1 cut(s) 133
SexAI ACCWGGT 1 cut(s) 162
SfaNI GCATC 6 cut(s) 244, 351, 612, 1240, 1391, 1438
SfcI CTRYAG 4 cut(s) 304, 859, 1596, 1851
Sfr274I CTCGAG 1 cut(s) 2021
SinI GGWCC 4 cut(s) 928, 969, 2005, 2157
SlaI CTCGAG 1 cut(s) 2021
SmiMI CAYNNNNRTG 5 cut(s) 720, 1795, 1949, 2090, 2314
SmlI CTYRAG 4 cut(s) 1236, 1421, 1434, 2021
SmoI CTYRAG 4 cut(s) 1236, 1421, 1434, 2021
SseBI AGGCCT 1 cut(s) 439
SsiI CCGC 9 cut(s) 35, 299, 311, 425, 669, 735, 1703, 1745, 1835
SspMI CTAG 5 cut(s) 524, 1223, 1286, 1841, 1947
StuI AGGCCT 1 cut(s) 439
StyD4I CCNGG 6 cut(s) 6, 162, 1479, 1590, 1879, 2264
StyI CCWWGG 3 cut(s) 111, 699, 2091
TaaI ACNGT 5 cut(s) 419, 1496, 1537, 1831, 1852
TaiI ACGT 3 cut(s) 663, 729, 1364
TaqI TCGA 6 cut(s) 1028, 1443, 1544, 2022, 2145, 2239
TaqII GACCGA 2 cut(s) 311, 1993
TatI WGTACW 2 cut(s) 22, 1399
TauI GCSGC 4 cut(s) 314, 427, 672, 738
TfiI GAWTC 4 cut(s) 101, 833, 2070, 2137
Tru1I TTAA 6 cut(s) 990, 1254, 1452, 1488, 1637, 2259
Tru9I TTAA 6 cut(s) 990, 1254, 1452, 1488, 1637, 2259
TscAI CASTG 4 cut(s) 187, 264, 334, 363
TseFI GTSAC 2 cut(s) 323, 1387
Tsp45I GTSAC 2 cut(s) 323, 1387
TspGWI ACGGA 6 cut(s) 159, 442, 459, 680, 755, 1020
TspRI CASTG 4 cut(s) 187, 264, 334, 363
Tth111I GACNNNGTC 2 cut(s) 335, 2161
Van91I CCANNNNNTGG 1 cut(s) 1780
VpaK11BI GGWCC 4 cut(s) 928, 969, 2005, 2157
VspI ATTAAT 1 cut(s) 1637
XagI CCTNNNNNAGG 1 cut(s) 522
XapI RAATTY 2 cut(s) 79, 341
XceI RCATGY 1 cut(s) 1451
XhoI CTCGAG 1 cut(s) 2021
XmiI GTMKAC 2 cut(s) 1115, 1364
XmnI GAANNNNTTC 1 cut(s) 212
XspI CTAG 5 cut(s) 524, 1223, 1286, 1841, 1947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.