RLG00000030980

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000724
Physical Location & Seq
Forward (+)
1137745 .. 1139873
2129 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030980

Sequence Viewer

Length: 1530 bp
ATGGAAGAAATACGTATTTCCGAACAATCCAAACCCCTCTTTCAAAGCCTCAGAGACGGTGATGTATTAGAAGTGGTGAAAGCTTATGCAAAAGCTACTACTCTTGATGCAAGATTAAAGCTAATTCCAACAGTGCACGACGACACAATCCTCCATTTGGCGATTTACATGGGGCAAGAAAGCATCGCAATAGGAATCATCGAAATAAGCAGGCATAATGACGAGCTAATTAAAAAACGCAATGCGTTTGGAAACACAGCTCTTCATGAGGCTGCAGCAGCTGATTTTGTACGAGTTGTGGAGAAGTTGTTGGAAAAGGCACCGGCATTGCTGTCCATTCCAAACAAGAAAGGGGAAATGCCTCTCTATACAGCAGCTCAATTTGGCCAGAAAAAAAATTTCATTTTGCTGGCTGAAAAACTAAAAAACAATCATTGTGATCTTCGTCCTCATTTCTGCTCCCTATCTCTTTCCTACGAGTCTGTTGAACACATACGCAGTACCATTCTTCATGCTGCAATACATGCCGAGTTCTTTGAGCTTGCACTTTTGATAGCTAGAATGCCGGAGTATGCGAAGATTGTTCGACTAAAAGACAAAAATTTGAAGACGCCTCTTCAGCTCCTCTCATCCAATTCTTCAGCATTCGAGAGTGGAATGAAATGTGGACTGATCAAGAGGTTCATCCACAACTGTGCTCCATATGAAGATGCTAAAAAAGAAGATGCTCATGAACAAAATTCCAGTGATAATAGTCATGTTCAAAGTTCCAAAAGATCAGCTACTCTGTTTCTAAGGCGAGTCTTCTCTTCAGTTAATAGATCGGTTTGGATTGTTCTTCGAGCATGGAGTACGATGAGGAGCATCTACAACGAAAAGAGGAGGCATGGATTGGCTTTAGAACTAGCCACGTTTCTAATTGCAGCAGACTTTTCATGGGATGTCACACGACTAGCATTAAATAATGAAAGTATTATGCAGACTGCTGTGGGACTCCCATTCCCAGATTCCGAAGACTCAGGTGGTTCCAGTAACACTAGCACTGAAGACCTCTCGGCTCCATCCCTGGAAAAGAGTACTCCACCAGCTATCCCATTGCACATTGCAACAGAGCATGGAATATTAGAAATTGTGAAGGAGACTCTTAGAGTCCACCCTGCGGCATACGAGTATAGAAATACCAAGGGACAAAACATACTGCACCTGTCCATTATGCACCGTCAGCGGGACATCTTTGATCTTGTAATGAAGAAGGAGACACCTAAGTCGAGGTTGCAACGACAACTCGATATTAATGACTTCACAGTATTGCACCAAGTTGGCAACATAGAGCATTACAATGGAGGTACCCAGCCTGGTCCTGCACTCCAATTGCAGGAAGAGCTGGAGTGGTTCGAGAAGGTGGAGGGGTTAATCCCACTACATTATGAAAAATTCAGTGCTTGTGAGACAGGCAAAGACACCACCCTACAGTCCTTTCATAAAAGATATGTAGAGATTCTCAAGGACTTGTCTGAGTGGATAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

510

Amino Acids

57.71

Weight (kDa)

6.9

Isoelectric Point (pI)

54.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 78 - 140 5.1e-06 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1264
Acc65I GGTACC 1 cut(s) 1346
AccB1I GGYRCC 2 cut(s) 319, 1346
AciI CCGC 2 cut(s) 1160, 1225
AcoI YGGCCR 1 cut(s) 385
AcsI RAATTY 4 cut(s) 397, 601, 739, 1433
AcuI CTGAAG 4 cut(s) 602, 624, 795, 1065
AcyI GRCGYC 1 cut(s) 611
AfaI GTAC 5 cut(s) 291, 502, 853, 1078, 1348
AfiI CCNNNNNNNGG 4 cut(s) 157, 1159, 1225, 1375
AgsI TTSAA 4 cut(s) 44, 488, 607, 764
AjnI CCWGG 2 cut(s) 1065, 1354
AleI CACNNNNGTG 1 cut(s) 693
Alw21I GWGCWC 2 cut(s) 138, 700
Alw26I GTCTC 4 cut(s) 48, 1133, 1250, 1442
Alw44I GTGCAC 1 cut(s) 134
AlwNI CAGNNNCTG 1 cut(s) 281
AoxI GGCC 1 cut(s) 385
ApaLI GTGCAC 1 cut(s) 134
ApeKI GCWGC 6 cut(s) 272, 275, 278, 374, 515, 923
ApoI RAATTY 4 cut(s) 397, 601, 739, 1433
AseI ATTAAT 1 cut(s) 1293
Asp718I GGTACC 1 cut(s) 1346
AspS9I GGNCC 1 cut(s) 1358
AsuHPI GGTGA 2 cut(s) 71, 88
AvaII GGWCC 1 cut(s) 1358
BaeGI GKGCMC 1 cut(s) 138
BalI TGGCCA 1 cut(s) 387
BanI GGYRCC 2 cut(s) 319, 1346
BbsI GAAGAC 4 cut(s) 614, 796, 1020, 1053
Bbv12I GWGCWC 2 cut(s) 138, 700
BbvI GCAGC 6 cut(s) 259, 287, 290, 386, 502, 935
BccI CCATC 1 cut(s) 1069
BciT130I CCWGG 2 cut(s) 1067, 1356
BclI TGATCA 1 cut(s) 672
BcoDI GTCTC 4 cut(s) 48, 1133, 1250, 1442
BfaI CTAG 4 cut(s) 558, 905, 953, 1038
BfmI CTRYAG 2 cut(s) 273, 1469
BisI GCNGC 7 cut(s) 273, 276, 279, 375, 516, 924, 1161
BlsI GCNGC 7 cut(s) 274, 277, 280, 376, 517, 925, 1162
BmcAI AGTACT 1 cut(s) 1078
Bme1390I CCNGG 2 cut(s) 1067, 1356
Bme18I GGWCC 1 cut(s) 1358
BmgT120I GGNCC 1 cut(s) 1358
BmiI GGNNCC 4 cut(s) 321, 1027, 1059, 1348
BmrFI CCNGG 2 cut(s) 1067, 1356
BmsI GCATC 5 cut(s) 97, 192, 700, 715, 873
BpiI GAAGAC 4 cut(s) 614, 796, 1020, 1053
BpmI CTGGAG 1 cut(s) 1406
BpuEI CTTGAG 1 cut(s) 1487
BsaAI YACGTR 1 cut(s) 14
BsaHI GRCGYC 1 cut(s) 611
BsaJI CCNNGG 2 cut(s) 1065, 1182
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 4 cut(s) 157, 1159, 1225, 1375
Bse118I RCCGGY 1 cut(s) 322
Bse1I ACTGG 2 cut(s) 744, 1029
Bse3DI GCAATG 4 cut(s) 247, 326, 1094, 1101
BseBI CCWGG 2 cut(s) 1067, 1356
BseDI CCNNGG 2 cut(s) 1065, 1182
BseGI GGATG 4 cut(s) 629, 684, 946, 1061
BseLI CCNNNNNNNGG 4 cut(s) 157, 1159, 1225, 1375
BseMI GCAATG 4 cut(s) 247, 326, 1094, 1101
BseMII CTCAG 3 cut(s) 64, 1032, 1506
BseNI ACTGG 2 cut(s) 744, 1029
BseRI GAGGAG 3 cut(s) 614, 874, 895
BseSI GKGCMC 1 cut(s) 138
BseXI GCAGC 6 cut(s) 259, 287, 290, 386, 502, 935
BseYI CCCAGC 1 cut(s) 1350
BsgI GTGCAG 2 cut(s) 1184, 1347
BshFI GGCC 1 cut(s) 387
BshNI GGYRCC 2 cut(s) 319, 1346
BsiHKAI GWGCWC 2 cut(s) 138, 700
BsiSI CCGG 2 cut(s) 323, 566
BslFI GGGAC 3 cut(s) 1005, 1200, 1241
BslI CCNNNNNNNGG 4 cut(s) 157, 1159, 1225, 1375
BsmAI GTCTC 4 cut(s) 48, 1133, 1250, 1442
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 3 cut(s) 1005, 1200, 1241
BsmI GAATGC 2 cut(s) 567, 644
BsnI GGCC 1 cut(s) 387
Bsp1286I GDGCHC 2 cut(s) 138, 700
Bsp143I GATC 5 cut(s) 439, 672, 776, 821, 1237
BspACI CCGC 2 cut(s) 1160, 1225
BspANI GGCC 1 cut(s) 387
BspCNI CTCAG 3 cut(s) 63, 1031, 1507
BspHI TCATGA 2 cut(s) 265, 730
BspLI GGNNCC 4 cut(s) 321, 1027, 1059, 1348
BspMAI CTGCAG 1 cut(s) 277
BspQI GCTCTTC 2 cut(s) 267, 1374
BspT107I GGYRCC 2 cut(s) 319, 1346
BsrDI GCAATG 4 cut(s) 247, 326, 1094, 1101
BsrFI RCCGGY 1 cut(s) 322
BsrI ACTGG 2 cut(s) 744, 1029
BssAI RCCGGY 1 cut(s) 322
BssECI CCNNGG 2 cut(s) 1065, 1182
BssMI GATC 5 cut(s) 439, 672, 776, 821, 1237
BssNI GRCGYC 1 cut(s) 611
BssT1I CCWWGG 1 cut(s) 1182
Bst2UI CCWGG 2 cut(s) 1067, 1356
Bst4CI ACNGT 6 cut(s) 59, 133, 695, 1220, 1306, 1473
Bst6I CTCTTC 4 cut(s) 267, 621, 814, 1374
BstACI GRCGYC 1 cut(s) 611
BstAPI GCANNNNNTGC 1 cut(s) 524
BstBAI YACGTR 1 cut(s) 14
BstC8I GCNNGC 3 cut(s) 212, 411, 543
BstDEI CTNAG 6 cut(s) 50, 794, 1018, 1145, 1263, 1515
BstF5I GGATG 4 cut(s) 629, 684, 946, 1061
BstKTI GATC 5 cut(s) 442, 675, 779, 824, 1240
BstMAI GTCTC 4 cut(s) 48, 1133, 1250, 1442
BstMBI GATC 5 cut(s) 439, 672, 776, 821, 1237
BstMWI GCNNNNNNNGC 5 cut(s) 278, 524, 619, 1222, 1381
BstNI CCWGG 2 cut(s) 1067, 1356
BstNSI RCATGY 1 cut(s) 527
BstSCI CCNGG 2 cut(s) 1065, 1354
BstSFI CTRYAG 2 cut(s) 273, 1469
BstSLI GKGCMC 1 cut(s) 138
BstSNI TACGTA 1 cut(s) 14
BstV1I GCAGC 6 cut(s) 259, 287, 290, 386, 502, 935
BstV2I GAAGAC 4 cut(s) 614, 796, 1020, 1053
BsuRI GGCC 1 cut(s) 387
BtgZI GCGATG 1 cut(s) 169
BtsCI GGATG 4 cut(s) 629, 684, 946, 1061
BtsIMutI CAGTG 4 cut(s) 138, 751, 1041, 1444
Cac8I GCNNGC 3 cut(s) 212, 411, 543
CaiI CAGNNNCTG 1 cut(s) 281
CciI TCATGA 2 cut(s) 265, 730
Cfr10I RCCGGY 1 cut(s) 322
Cfr13I GGNCC 1 cut(s) 1358
CseI GACGC 1 cut(s) 619
Csp6I GTAC 5 cut(s) 290, 501, 852, 1077, 1347
CviQI GTAC 5 cut(s) 290, 501, 852, 1077, 1347
DdeI CTNAG 6 cut(s) 50, 794, 1018, 1145, 1263, 1515
DpnI GATC 5 cut(s) 441, 674, 778, 823, 1239
DpnII GATC 5 cut(s) 439, 672, 776, 821, 1237
DrdI GACNNNNNNGTC 1 cut(s) 1264
DseDI GACNNNNNNGTC 1 cut(s) 1264
EaeI YGGCCR 1 cut(s) 385
Eam1104I CTCTTC 4 cut(s) 267, 621, 814, 1374
EarI CTCTTC 4 cut(s) 267, 621, 814, 1374
Eco105I TACGTA 1 cut(s) 14
Eco130I CCWWGG 1 cut(s) 1182
Eco47I GGWCC 1 cut(s) 1358
Eco57I CTGAAG 4 cut(s) 602, 624, 795, 1065
EcoRII CCWGG 2 cut(s) 1065, 1354
EcoT14I CCWWGG 1 cut(s) 1182
ErhI CCWWGG 1 cut(s) 1182
Esp3I CGTCTC 1 cut(s) 48
FaqI GGGAC 3 cut(s) 1005, 1200, 1241
FauI CCCGC 1 cut(s) 1218
FauNDI CATATG 1 cut(s) 703
FbaI TGATCA 1 cut(s) 672
Fnu4HI GCNGC 7 cut(s) 273, 276, 279, 375, 516, 924, 1161
FokI GGATG 4 cut(s) 616, 671, 953, 1048
Fsp4HI GCNGC 7 cut(s) 273, 276, 279, 375, 516, 924, 1161
FspBI CTAG 4 cut(s) 558, 905, 953, 1038
GluI GCNGC 7 cut(s) 273, 276, 279, 375, 516, 924, 1161
GsaI CCCAGC 1 cut(s) 1354
GsuI CTGGAG 1 cut(s) 1406
HaeIII GGCC 1 cut(s) 387
HapII CCGG 2 cut(s) 323, 566
HgaI GACGC 1 cut(s) 619
Hin1I GRCGYC 1 cut(s) 611
HindIII AAGCTT 1 cut(s) 81
HinfI GANTC 9 cut(s) 195, 479, 801, 993, 1007, 1016, 1141, 1149, 1498
HpaII CCGG 2 cut(s) 323, 566
HphI GGTGA 2 cut(s) 71, 88
Hpy166II GTNNAC 3 cut(s) 136, 668, 1153
Hpy188I TCNGA 4 cut(s) 22, 53, 1012, 1516
Hpy188III TCNNGA 6 cut(s) 104, 266, 649, 676, 731, 1396
Hpy8I GTNNAC 3 cut(s) 136, 668, 1153
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 3 cut(s) 1129, 1246, 1393
HpyCH4III ACNGT 6 cut(s) 59, 133, 695, 1220, 1306, 1473
HpyCH4IV ACGT 2 cut(s) 13, 911
HpyF10VI GCNNNNNNNGC 5 cut(s) 278, 524, 619, 1222, 1381
HpyF3I CTNAG 6 cut(s) 50, 794, 1018, 1145, 1263, 1515
HpySE526I ACGT 2 cut(s) 13, 911
Hsp92I GRCGYC 1 cut(s) 611
KpnI GGTACC 1 cut(s) 1350
Ksp22I TGATCA 1 cut(s) 672
Kzo9I GATC 5 cut(s) 439, 672, 776, 821, 1237
LguI GCTCTTC 2 cut(s) 267, 1374
LmnI GCTCC 5 cut(s) 464, 627, 703, 861, 1063
Lsp1109I GCAGC 6 cut(s) 259, 287, 290, 386, 502, 935
LweI GCATC 5 cut(s) 97, 192, 700, 715, 873
MaeI CTAG 4 cut(s) 558, 905, 953, 1038
MaeII ACGT 2 cut(s) 13, 911
MaeIII GTNAC 2 cut(s) 943, 1031
MalI GATC 5 cut(s) 441, 674, 778, 823, 1239
MboI GATC 5 cut(s) 439, 672, 776, 821, 1237
MfeI CAATTG 1 cut(s) 1370
MhlI GDGCHC 2 cut(s) 138, 700
MlsI TGGCCA 1 cut(s) 387
MluNI TGGCCA 1 cut(s) 387
MlyI GAGTC 6 cut(s) 488, 810, 987, 1010, 1135, 1158
MmeI TCCRAC 2 cut(s) 152, 291
Mox20I TGGCCA 1 cut(s) 387
MscI TGGCCA 1 cut(s) 387
MseI TTAA 6 cut(s) 116, 231, 816, 959, 1293, 1412
MslI CAYNNNNRTG 2 cut(s) 693, 1338
Msp20I TGGCCA 1 cut(s) 387
MspA1I CMGCKG 2 cut(s) 281, 1225
MspI CCGG 2 cut(s) 323, 566
MspR9I CCNGG 2 cut(s) 1067, 1356
MunI CAATTG 1 cut(s) 1370
Mva1269I GAATGC 2 cut(s) 567, 644
MvaI CCWGG 2 cut(s) 1067, 1356
MwoI GCNNNNNNNGC 5 cut(s) 278, 524, 619, 1222, 1381
NdeI CATATG 1 cut(s) 703
NdeII GATC 5 cut(s) 439, 672, 776, 821, 1237
NlaIV GGNNCC 4 cut(s) 321, 1027, 1059, 1348
NmeAIII GCCGAG 2 cut(s) 553, 1034
NmuCI GTSAC 1 cut(s) 943
NspI RCATGY 1 cut(s) 527
OliI CACNNNNGTG 1 cut(s) 693
PagI TCATGA 2 cut(s) 265, 730
PciSI GCTCTTC 2 cut(s) 267, 1374
PctI GAATGC 2 cut(s) 567, 644
PfeI GAWTC 3 cut(s) 195, 1007, 1498
PkrI GCNGC 7 cut(s) 274, 277, 280, 376, 517, 925, 1162
PleI GAGTC 6 cut(s) 487, 809, 987, 1010, 1135, 1157
PpsI GAGTC 6 cut(s) 487, 809, 987, 1010, 1135, 1157
Ppu21I YACGTR 1 cut(s) 14
PshBI ATTAAT 1 cut(s) 1293
Psp6I CCWGG 2 cut(s) 1065, 1354
PspFI CCCAGC 1 cut(s) 1350
PspGI CCWGG 2 cut(s) 1065, 1354
PspN4I GGNNCC 4 cut(s) 321, 1027, 1059, 1348
PspPI GGNCC 1 cut(s) 1358
PstI CTGCAG 1 cut(s) 277
PstNI CAGNNNCTG 1 cut(s) 281
PvuII CAGCTG 1 cut(s) 281
RsaI GTAC 5 cut(s) 291, 502, 853, 1078, 1348
RsaNI GTAC 5 cut(s) 290, 501, 852, 1077, 1347
RseI CAYNNNNRTG 2 cut(s) 693, 1338
SapI GCTCTTC 2 cut(s) 267, 1374
SaqAI TTAA 6 cut(s) 116, 231, 816, 959, 1293, 1412
SatI GCNGC 7 cut(s) 273, 276, 279, 375, 516, 924, 1161
Sau3AI GATC 5 cut(s) 439, 672, 776, 821, 1237
Sau96I GGNCC 1 cut(s) 1358
ScaI AGTACT 1 cut(s) 1078
SchI GAGTC 6 cut(s) 488, 810, 987, 1010, 1135, 1158
ScrFI CCNGG 2 cut(s) 1067, 1356
SduI GDGCHC 2 cut(s) 138, 700
SfaNI GCATC 5 cut(s) 97, 192, 700, 715, 873
SfcI CTRYAG 2 cut(s) 273, 1469
SinI GGWCC 1 cut(s) 1358
SmiMI CAYNNNNRTG 2 cut(s) 693, 1338
SmlI CTYRAG 1 cut(s) 1502
SmoI CTYRAG 1 cut(s) 1502
SnaBI TACGTA 1 cut(s) 14
SsiI CCGC 2 cut(s) 1160, 1225
SspI AATATT 1 cut(s) 1122
SspMI CTAG 4 cut(s) 558, 905, 953, 1038
StyD4I CCNGG 2 cut(s) 1065, 1354
StyI CCWWGG 1 cut(s) 1182
TaaI ACNGT 6 cut(s) 59, 133, 695, 1220, 1306, 1473
TaiI ACGT 2 cut(s) 16, 914
TaqI TCGA 7 cut(s) 201, 586, 648, 841, 1268, 1287, 1395
TatI WGTACW 1 cut(s) 1076
TauI GCSGC 1 cut(s) 1163
TfiI GAWTC 3 cut(s) 195, 1007, 1498
Tru1I TTAA 6 cut(s) 116, 231, 816, 959, 1293, 1412
Tru9I TTAA 6 cut(s) 116, 231, 816, 959, 1293, 1412
TscAI CASTG 4 cut(s) 138, 751, 1048, 1444
TseFI GTSAC 1 cut(s) 943
TseI GCWGC 6 cut(s) 272, 275, 278, 374, 515, 923
Tsp45I GTSAC 1 cut(s) 943
TspRI CASTG 4 cut(s) 138, 751, 1048, 1444
VneI GTGCAC 1 cut(s) 134
VpaK11BI GGWCC 1 cut(s) 1358
VspI ATTAAT 1 cut(s) 1293
XapI RAATTY 4 cut(s) 397, 601, 739, 1433
XceI RCATGY 1 cut(s) 527
XspI CTAG 4 cut(s) 558, 905, 953, 1038
ZrmI AGTACT 1 cut(s) 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.