pycom10g27740

Ankyrin repeat-containing protein At3g12360-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000724
Physical Location & Seq
Reverse (-)
28627279 .. 28630232
2954 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g27740.5

Sequence Viewer

Length: 2127 bp
ATGGATTACTGGAAAACGGTGGATCGGAGTGATCAAAACAAGAGAGAGAGTGAATTGAAAAAAATTGAAGACTCCAAAGGTCCCTATTTATGTGCCATGGAGGATGACTGGGAAGGCGTGACAAATTACTACAAGGGCAAACCAGGCGAATTGCTCATTCCAATGACGGTGGGCAGGGACACTGCATTTCACCTTGCGGCTTCTTGCTGCAGCAAATCACAAGCCAAAAGAATCCTCGAAATGTTGATCAATATACTAACCACCAGCTATGACAAGAGACGCGCGGTGAGGAAACTGAATAACACCGGAAACAATACTCTTCACGACGTGAGTTTGTCTGGAAGTGTGGAAGCGGCAGAGTACTTGGTGAGCAACTTCAACGAGCCTGTTGGACTTGAAGGCGAGGTCATCATCAGTAATGATGCGCTGCCGCTGCTGGAGACTCGGAACCATTTAGGAGAAACTCCGCTCTTCAGGGCGGCTGTTCTTGGTCACACTGACTTGGTCAAGTTTTATGCTCGCAAACTGGAGGAGGATAATCCGGACAATCTTTGGAGGCACTTCCACAGAAAGGACAAAATGTCCATTCTTCATATGGCAGTCATCGGGCAACATTTCGGTCTCTCTCTGAAAATGCACACTTTGATTCTTAATATTACTGCTCTTTGGTTACTGGAGAACTATTATAACTACCTAGCGCACCAAAAAGAAGATAAAGGATTGACATGCCTTCAATTGCTAGCCCAAATGCCAACTGCCTTCCAGCCACAATTTCAGCAAAGCATATGGAAGATGCTCATTTACTATTGTATGCACGGCCTTCCTGATGGAGGAGATGTGGTCACACGCCTTCCTGATGGAGGAGATGTGGTCACACGCAATCAGAAGGATGATGTAAAGATCAACTTAGATAGCAACCAGACGCTCCGTCAGCCTCTCTCTCGGAACAAGCTTGCAGGGTACAATCTCAGTAAGAAAAGAAAGGCGACCGCAAAAGCATCCAAATCGGATAAAGGAAAAGGTGGTGATGGAGGTGAAAAAGGACCGGAAGCTGCAGAAACATCCGAATCGGGTAAGGGTAAAGGTGATGATGGAGGTGAAAGAGGATTGGAAGCCGCAAAAACATCCAAATCGGATAAGGGAAAAGGTGATGATGGAGGTGAAAAAGGACCGGAAGCCGCAACAACCATTTCAGATAATGCACCGAGGTATAAAGTTTACAACTTTACACCATTGCTTATAGCAACCATCACAGGAATTATGCCCATTGTGGAGGAGATACTTGAGCAGCATCCTCAGGCAGTCGAGCACGTTAACTATGACGAACGCAACATATTGCATCTAGCCATTAGGAACCGTCGGAAAGAGATCCTTAATCTTATCAAAAGCAAACCAACCGTGATGTTGAGGCTGAAGGAGTGGATAGACTGCGATGGCAACACCATATTGCACCAGGCTGCGGATAGGGGTTATTACACTAAAGCGTTGTCTCAGAAACTAATTGGCCCTGCAATGCAATTGCAAGAAGAGCTACGTTGGACGCTGTGTGTAAAAGAGATACTACCGCCTCGGTACATCTTGCACCGCAACAAGAAGGATCAGACAGCTGAGGAGCTGTTCAACTACCAGCATGAGGGTCTTCTGGGGTCGGCACAAGAATGGGTAAAAGAAACGGCTCAGTCATGCTCAACTGTGGCGGTACTAGTGGCCACTGTGGTCTTTGCAGCTGCCTACGCCATTCCCGGGGGTACTAGTGACCAAAATGGCCTTCCTCTTTTCCAAGACGATCCTCTCTTTTTGTTCTTCGCCTGCATGAACGTTCTGGGCATCGCCTGCTCATTATCTTCAGTGGCATTCTTTCTCTCGGTCCTCTCCTCCCCTCTCGAGTACCCATATTTCGTTAAAAGTCTTCCTCGCAAGATCGATGACTGGATTCATCTTGCTCTTCTTGTCAATGGCAGCCACTATGCTCGCTTTTGCCGCCACCATTTTGCTCTTGATGAGTGTAGAGAAGAAATGGACCAAGTCTATACTTTACCCCATTGCATTTTTCCCAGTCCCTCTATTTGGCCTGCTGCAATTTCCTATGTATCAATCTTTCATAGTCATGTTTCGGAAAATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

709

Amino Acids

79.51

Weight (kDa)

6.57

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 687
AccBSI CCGCTC 1 cut(s) 469
AccII CGCG 2 cut(s) 282, 284
AccIII TCCGGA 1 cut(s) 541
AclI AACGTT 1 cut(s) 1818
AclWI GGATC 4 cut(s) 30, 1363, 1605, 1781
AcoI YGGCCR 1 cut(s) 1707
AcuI CTGAAG 3 cut(s) 457, 1433, 1830
AdeI CACNNNGTG 1 cut(s) 328
AfaI GTAC 6 cut(s) 362, 962, 1574, 1701, 1750, 1889
AfiI CCNNNNNNNGG 7 cut(s) 571, 830, 860, 1459, 1633, 1743, 2067
AgsI TTSAA 6 cut(s) 58, 68, 379, 398, 734, 1621
AhdI GACNNNNNGTC 2 cut(s) 580, 927
AhlI ACTAGT 2 cut(s) 1702, 1751
AjiI CACGTC 1 cut(s) 328
AjnI CCWGG 2 cut(s) 142, 1452
AjuI GAANNNNNNNTTGG 2 cut(s) 1752, 1784
AluBI AGCT 7 cut(s) 267, 952, 1052, 1531, 1607, 1615, 1727
AluI AGCT 7 cut(s) 267, 952, 1052, 1531, 1607, 1615, 1727
Alw21I GWGCWC 1 cut(s) 1311
Alw26I GTCTC 4 cut(s) 271, 434, 626, 1494
AlwI GGATC 4 cut(s) 30, 1363, 1605, 1781
Ama87I CYCGRG 2 cut(s) 1742, 1883
Aor13HI TCCGGA 1 cut(s) 541
AoxI GGCC 5 cut(s) 817, 1504, 1707, 1765, 2069
AspLEI GCGC 3 cut(s) 284, 427, 700
AspS9I GGNCC 6 cut(s) 80, 1043, 1169, 1505, 1867, 2020
AsuC2I CCSGG 2 cut(s) 1743, 1744
AsuHPI GGTGA 9 cut(s) 182, 298, 379, 1037, 1046, 1097, 1109, 1160, 1172
AsuNHI GCTAGC 1 cut(s) 739
AvaI CYCGRG 2 cut(s) 1742, 1883
AvaII GGWCC 5 cut(s) 80, 1043, 1169, 1867, 2020
AxyI CCTNAGG 1 cut(s) 1296
BalI TGGCCA 1 cut(s) 1709
BbsI GAAGAC 3 cut(s) 75, 1631, 1901
Bbv12I GWGCWC 1 cut(s) 1311
BbvCI CCTCAGC 1 cut(s) 1608
BccI CCATC 7 cut(s) 821, 851, 1022, 1085, 1148, 1256, 1427
BceAI ACGGC 2 cut(s) 832, 1689
BcgI CGANNNNNNTGC 2 cut(s) 987, 1021
BciT130I CCWGG 2 cut(s) 144, 1454
BclI TGATCA 2 cut(s) 31, 246
BcnI CCSGG 2 cut(s) 1743, 1744
BcoDI GTCTC 4 cut(s) 271, 434, 626, 1494
BcuI ACTAGT 2 cut(s) 1702, 1751
BfaI CTAG 5 cut(s) 695, 740, 1343, 1703, 1752
BfmI CTRYAG 2 cut(s) 208, 1053
BmcAI AGTACT 1 cut(s) 362
Bme1390I CCNGG 4 cut(s) 144, 1454, 1743, 1744
Bme18I GGWCC 5 cut(s) 80, 1043, 1169, 1867, 2020
BmeRI GACNNNNNGTC 2 cut(s) 580, 927
BmeT110I CYCGRG 2 cut(s) 1742, 1883
BmgBI CACGTC 1 cut(s) 328
BmgT120I GGNCC 6 cut(s) 80, 1043, 1169, 1505, 1867, 2020
BmiI GGNNCC 3 cut(s) 82, 449, 1355
BmrFI CCNGG 4 cut(s) 144, 1454, 1743, 1744
BmrI ACTGGG 2 cut(s) 118, 2049
BmsI GCATC 6 cut(s) 412, 783, 1007, 1300, 1348, 1836
BmtI GCTAGC 1 cut(s) 743
BmuI ACTGGG 2 cut(s) 118, 2049
BpiI GAAGAC 3 cut(s) 75, 1631, 1901
BpmI CTGGAG 3 cut(s) 458, 548, 695
Bpu10I CCTNAGC 1 cut(s) 1608
BpuEI CTTGAG 1 cut(s) 1304
BpuMI CCSGG 2 cut(s) 1743, 1744
Bsa29I ATCGAT 2 cut(s) 1923, 2122
BsaI GGTCTC 1 cut(s) 626
BsaJI CCNNGG 5 cut(s) 96, 1205, 1568, 1742, 1743
BsaWI WCCGGW 4 cut(s) 305, 541, 1045, 1171
BsaXI ACNNNNNCTCC 8 cut(s) 822, 825, 852, 852, 855, 855, 882, 885
Bsc4I CCNNNNNNNGG 7 cut(s) 571, 830, 860, 1459, 1633, 1743, 2067
Bse1I ACTGG 6 cut(s) 14, 113, 531, 678, 1934, 2055
Bse21I CCTNAGG 1 cut(s) 1296
Bse3DI GCAATG 3 cut(s) 1232, 1518, 2041
BseAI TCCGGA 1 cut(s) 541
BseBI CCWGG 2 cut(s) 144, 1454
BseCI ATCGAT 2 cut(s) 1923, 2122
BseDI CCNNGG 5 cut(s) 96, 1205, 1568, 1742, 1743
BseGI GGATG 6 cut(s) 109, 895, 998, 1061, 1124, 1291
BseLI CCNNNNNNNGG 7 cut(s) 571, 830, 860, 1459, 1633, 1743, 2067
BseMI GCAATG 3 cut(s) 1232, 1518, 2041
BseMII CTCAG 5 cut(s) 982, 1310, 1505, 1599, 1691
BseNI ACTGG 6 cut(s) 14, 113, 531, 678, 1934, 2055
BseRI GAGGAG 6 cut(s) 545, 846, 876, 1289, 1625, 1864
Bsh1236I CGCG 2 cut(s) 282, 284
Bsh1285I CGRYCG 1 cut(s) 990
BshFI GGCC 5 cut(s) 819, 1506, 1709, 1767, 2071
BshVI ATCGAT 2 cut(s) 1923, 2122
BsiEI CGRYCG 1 cut(s) 990
BsiHKAI GWGCWC 1 cut(s) 1311
BsiHKCI CYCGRG 2 cut(s) 1742, 1883
BsiSI CCGG 5 cut(s) 306, 542, 1046, 1172, 1743
BslFI GGGAC 3 cut(s) 66, 191, 2043
BslI CCNNNNNNNGG 7 cut(s) 571, 830, 860, 1459, 1633, 1743, 2067
BsmAI GTCTC 4 cut(s) 271, 434, 626, 1494
BsmBI CGTCTC 1 cut(s) 271
BsmFI GGGAC 3 cut(s) 66, 191, 2043
BsmI GAATGC 1 cut(s) 1853
BsnI GGCC 5 cut(s) 819, 1506, 1709, 1767, 2071
Bso31I GGTCTC 1 cut(s) 626
BsoBI CYCGRG 2 cut(s) 1742, 1883
Bsp1286I GDGCHC 1 cut(s) 1311
Bsp13I TCCGGA 1 cut(s) 541
Bsp143I GATC 8 cut(s) 22, 31, 246, 900, 1368, 1597, 1786, 1920
Bsp19I CCATGG 1 cut(s) 96
BspANI GGCC 5 cut(s) 819, 1506, 1709, 1767, 2071
BspCNI CTCAG 5 cut(s) 981, 1309, 1504, 1600, 1690
BspDI ATCGAT 2 cut(s) 1923, 2122
BspEI TCCGGA 1 cut(s) 541
BspFNI CGCG 2 cut(s) 282, 284
BspLI GGNNCC 3 cut(s) 82, 449, 1355
BspMAI CTGCAG 2 cut(s) 212, 1057
BspOI GCTAGC 1 cut(s) 743
BspPI GGATC 4 cut(s) 30, 1363, 1605, 1781
BspQI GCTCTTC 3 cut(s) 476, 1521, 1950
BspTNI GGTCTC 1 cut(s) 626
BsrBI CCGCTC 1 cut(s) 469
BsrDI GCAATG 3 cut(s) 1232, 1518, 2041
BsrI ACTGG 6 cut(s) 14, 113, 531, 678, 1934, 2055
BssECI CCNNGG 5 cut(s) 96, 1205, 1568, 1742, 1743
BssMI GATC 8 cut(s) 22, 31, 246, 900, 1368, 1597, 1786, 1920
BssT1I CCWWGG 1 cut(s) 96
Bst2UI CCWGG 2 cut(s) 144, 1454
Bst4CI ACNGT 6 cut(s) 19, 169, 1358, 1399, 1693, 1714
Bst6I CTCTTC 4 cut(s) 324, 476, 1521, 1950
BstAPI GCANNNNNTGC 1 cut(s) 1833
BstC8I GCNNGC 7 cut(s) 520, 741, 954, 1810, 1834, 1972, 2073
BstDEI CTNAG 6 cut(s) 907, 968, 1296, 1491, 1608, 1677
BstDSI CCRYGG 1 cut(s) 96
BstENI CCTNNNNNAGG 2 cut(s) 828, 858
BstF5I GGATG 6 cut(s) 109, 895, 998, 1061, 1124, 1291
BstFNI CGCG 2 cut(s) 282, 284
BstHHI GCGC 3 cut(s) 284, 427, 700
BstKTI GATC 8 cut(s) 25, 34, 249, 903, 1371, 1600, 1789, 1923
BstMAI GTCTC 4 cut(s) 271, 434, 626, 1494
BstMBI GATC 8 cut(s) 22, 31, 246, 900, 1368, 1597, 1786, 1920
BstMCI CGRYCG 1 cut(s) 990
BstMWI GCNNNNNNNGC 7 cut(s) 144, 433, 931, 1528, 1733, 1833, 1980
BstNI CCWGG 2 cut(s) 144, 1454
BstNSI RCATGY 1 cut(s) 729
BstSCI CCNGG 4 cut(s) 142, 1452, 1741, 1742
BstSFI CTRYAG 2 cut(s) 208, 1053
BstUI CGCG 2 cut(s) 282, 284
BstV2I GAAGAC 3 cut(s) 75, 1631, 1901
BstX2I RGATCY 1 cut(s) 1368
BstYI RGATCY 1 cut(s) 1368
Bsu15I ATCGAT 2 cut(s) 1923, 2122
Bsu36I CCTNAGG 1 cut(s) 1296
BsuRI GGCC 5 cut(s) 819, 1506, 1709, 1767, 2071
BsuTUI ATCGAT 2 cut(s) 1923, 2122
BtgI CCRYGG 1 cut(s) 96
BtgZI GCGATG 2 cut(s) 1446, 1813
BtrI CACGTC 1 cut(s) 328
BtsCI GGATG 6 cut(s) 109, 895, 998, 1061, 1124, 1291
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 4 cut(s) 180, 495, 1710, 1854
Cac8I GCNNGC 7 cut(s) 520, 741, 954, 1810, 1834, 1972, 2073
CfoI GCGC 3 cut(s) 284, 427, 700
Cfr13I GGNCC 6 cut(s) 80, 1043, 1169, 1505, 1867, 2020
Cfr9I CCCGGG 1 cut(s) 1742
ClaI ATCGAT 2 cut(s) 1923, 2122
CseI GACGC 3 cut(s) 288, 931, 1549
Csp6I GTAC 6 cut(s) 361, 961, 1573, 1700, 1749, 1888
CspCI CAANNNNNGTGG 2 cut(s) 150, 185
CviAII CATG 6 cut(s) 97, 726, 1631, 1683, 1813, 2108
CviQI GTAC 6 cut(s) 361, 961, 1573, 1700, 1749, 1888
DdeI CTNAG 6 cut(s) 907, 968, 1296, 1491, 1608, 1677
DpnI GATC 8 cut(s) 24, 33, 248, 902, 1370, 1599, 1788, 1922
DpnII GATC 8 cut(s) 22, 31, 246, 900, 1368, 1597, 1786, 1920
DraIII CACNNNGTG 1 cut(s) 328
DriI GACNNNNNGTC 2 cut(s) 580, 927
EaeI YGGCCR 1 cut(s) 1707
Eam1104I CTCTTC 4 cut(s) 324, 476, 1521, 1950
Eam1105I GACNNNNNGTC 2 cut(s) 580, 927
EarI CTCTTC 4 cut(s) 324, 476, 1521, 1950
Eco130I CCWWGG 1 cut(s) 96
Eco31I GGTCTC 1 cut(s) 626
Eco47I GGWCC 5 cut(s) 80, 1043, 1169, 1867, 2020
Eco57I CTGAAG 3 cut(s) 457, 1433, 1830
Eco81I CCTNAGG 1 cut(s) 1296
Eco88I CYCGRG 2 cut(s) 1742, 1883
EcoNI CCTNNNNNAGG 2 cut(s) 828, 858
EcoO109I RGGNCCY 1 cut(s) 80
EcoRII CCWGG 2 cut(s) 142, 1452
EcoT14I CCWWGG 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 96
Esp3I CGTCTC 1 cut(s) 271
FaeI CATG 6 cut(s) 100, 729, 1634, 1686, 1816, 2111
FalI AAGNNNNNCTT 4 cut(s) 890, 922, 1356, 1388
FaqI GGGAC 3 cut(s) 66, 191, 2043
FatI CATG 6 cut(s) 96, 725, 1630, 1682, 1812, 2107
FauNDI CATATG 2 cut(s) 594, 785
FbaI TGATCA 2 cut(s) 31, 246
FokI GGATG 6 cut(s) 116, 902, 985, 1048, 1111, 1278
FspBI CTAG 5 cut(s) 695, 740, 1343, 1703, 1752
GlaI GCGC 3 cut(s) 283, 426, 699
GsuI CTGGAG 3 cut(s) 458, 548, 695
HaeIII GGCC 5 cut(s) 819, 1506, 1709, 1767, 2071
HapII CCGG 5 cut(s) 306, 542, 1046, 1172, 1743
HgaI GACGC 3 cut(s) 288, 931, 1549
HhaI GCGC 3 cut(s) 284, 427, 700
Hin1II CATG 6 cut(s) 100, 729, 1634, 1686, 1816, 2111
Hin6I GCGC 3 cut(s) 282, 425, 698
HinP1I GCGC 3 cut(s) 282, 425, 698
HincII GTYRAC 1 cut(s) 1315
HindII GTYRAC 1 cut(s) 1315
HindIII AAGCTT 1 cut(s) 950
HinfI GANTC 6 cut(s) 71, 231, 442, 646, 1067, 1933
HpaI GTTAAC 1 cut(s) 1315
HpaII CCGG 5 cut(s) 306, 542, 1046, 1172, 1743
HphI GGTGA 9 cut(s) 182, 298, 379, 1037, 1046, 1097, 1109, 1160, 1172
Hpy166II GTNNAC 2 cut(s) 1219, 1315
Hpy188III TCNNGA 7 cut(s) 323, 339, 542, 824, 854, 1883, 1997
Hpy8I GTNNAC 2 cut(s) 1219, 1315
Hpy99I CGWCG 2 cut(s) 329, 1362
HpyCH4III ACNGT 6 cut(s) 19, 169, 1358, 1399, 1693, 1714
HpyCH4IV ACGT 4 cut(s) 327, 1311, 1534, 1818
HpyF10VI GCNNNNNNNGC 7 cut(s) 144, 433, 931, 1528, 1733, 1833, 1980
HpyF3I CTNAG 6 cut(s) 907, 968, 1296, 1491, 1608, 1677
HpySE526I ACGT 4 cut(s) 327, 1311, 1534, 1818
Hsp92II CATG 6 cut(s) 100, 729, 1634, 1686, 1816, 2111
HspAI GCGC 3 cut(s) 282, 425, 698
Kpn2I TCCGGA 1 cut(s) 541
Ksp22I TGATCA 2 cut(s) 31, 246
KspAI GTTAAC 1 cut(s) 1315
Kzo9I GATC 8 cut(s) 22, 31, 246, 900, 1368, 1597, 1786, 1920
LguI GCTCTTC 3 cut(s) 476, 1521, 1950
LmnI GCTCC 2 cut(s) 930, 1612
LweI GCATC 6 cut(s) 412, 783, 1007, 1300, 1348, 1836
MaeI CTAG 5 cut(s) 695, 740, 1343, 1703, 1752
MaeII ACGT 4 cut(s) 327, 1311, 1534, 1818
MaeIII GTNAC 6 cut(s) 118, 491, 669, 841, 871, 1754
MalI GATC 8 cut(s) 24, 33, 248, 902, 1370, 1599, 1788, 1922
MbiI CCGCTC 1 cut(s) 469
MboI GATC 8 cut(s) 22, 31, 246, 900, 1368, 1597, 1786, 1920
MfeI CAATTG 2 cut(s) 734, 1517
MflI RGATCY 1 cut(s) 1368
MhlI GDGCHC 1 cut(s) 1311
MlsI TGGCCA 1 cut(s) 1709
MluNI TGGCCA 1 cut(s) 1709
MlyI GAGTC 2 cut(s) 65, 436
MmeI TCCRAC 3 cut(s) 370, 1340, 1517
Mox20I TGGCCA 1 cut(s) 1709
MroI TCCGGA 1 cut(s) 541
MscI TGGCCA 1 cut(s) 1709
MseI TTAA 4 cut(s) 651, 1314, 1374, 1902
MslI CAYNNNNRTG 3 cut(s) 161, 1657, 2106
Msp20I TGGCCA 1 cut(s) 1709
MspA1I CMGCKG 3 cut(s) 433, 1607, 1727
MspI CCGG 5 cut(s) 306, 542, 1046, 1172, 1743
MspR9I CCNGG 4 cut(s) 144, 1454, 1743, 1744
MunI CAATTG 2 cut(s) 734, 1517
Mva1269I GAATGC 1 cut(s) 1853
MvaI CCWGG 2 cut(s) 144, 1454
MvnI CGCG 2 cut(s) 282, 284
MwoI GCNNNNNNNGC 7 cut(s) 144, 433, 931, 1528, 1733, 1833, 1980
NciI CCSGG 2 cut(s) 1743, 1744
NcoI CCATGG 1 cut(s) 96
NdeI CATATG 2 cut(s) 594, 785
NdeII GATC 8 cut(s) 22, 31, 246, 900, 1368, 1597, 1786, 1920
NheI GCTAGC 1 cut(s) 739
NlaIII CATG 6 cut(s) 100, 729, 1634, 1686, 1816, 2111
NlaIV GGNNCC 3 cut(s) 82, 449, 1355
NmuCI GTSAC 5 cut(s) 118, 491, 841, 871, 1754
NspI RCATGY 1 cut(s) 729
PaeR7I CTCGAG 1 cut(s) 1883
PciSI GCTCTTC 3 cut(s) 476, 1521, 1950
PctI GAATGC 1 cut(s) 1853
PfeI GAWTC 4 cut(s) 231, 646, 1067, 1933
PflFI GACNNNGTC 2 cut(s) 503, 2024
PleI GAGTC 2 cut(s) 65, 436
PpsI GAGTC 2 cut(s) 65, 436
PpuMI RGGWCCY 1 cut(s) 80
PsiI TTATAA 1 cut(s) 687
Psp1406I AACGTT 1 cut(s) 1818
Psp5II RGGWCCY 1 cut(s) 80
Psp6I CCWGG 2 cut(s) 142, 1452
PspGI CCWGG 2 cut(s) 142, 1452
PspN4I GGNNCC 3 cut(s) 82, 449, 1355
PspPI GGNCC 6 cut(s) 80, 1043, 1169, 1505, 1867, 2020
PspPPI RGGWCCY 1 cut(s) 80
PstI CTGCAG 2 cut(s) 212, 1057
PsuI RGATCY 1 cut(s) 1368
PsyI GACNNNGTC 2 cut(s) 503, 2024
PvuII CAGCTG 2 cut(s) 1607, 1727
RsaI GTAC 6 cut(s) 362, 962, 1574, 1701, 1750, 1889
RsaNI GTAC 6 cut(s) 361, 961, 1573, 1700, 1749, 1888
RseI CAYNNNNRTG 3 cut(s) 161, 1657, 2106
SapI GCTCTTC 3 cut(s) 476, 1521, 1950
SaqAI TTAA 4 cut(s) 651, 1314, 1374, 1902
Sau3AI GATC 8 cut(s) 22, 31, 246, 900, 1368, 1597, 1786, 1920
Sau96I GGNCC 6 cut(s) 80, 1043, 1169, 1505, 1867, 2020
ScaI AGTACT 1 cut(s) 362
SchI GAGTC 2 cut(s) 65, 436
ScrFI CCNGG 4 cut(s) 144, 1454, 1743, 1744
SduI GDGCHC 1 cut(s) 1311
SfaNI GCATC 6 cut(s) 412, 783, 1007, 1300, 1348, 1836
SfcI CTRYAG 2 cut(s) 208, 1053
Sfr274I CTCGAG 1 cut(s) 1883
SinI GGWCC 5 cut(s) 80, 1043, 1169, 1867, 2020
SlaI CTCGAG 1 cut(s) 1883
SmaI CCCGGG 1 cut(s) 1744
SmiMI CAYNNNNRTG 3 cut(s) 161, 1657, 2106
SmlI CTYRAG 2 cut(s) 1283, 1883
SmoI CTYRAG 2 cut(s) 1283, 1883
SpeI ACTAGT 2 cut(s) 1702, 1751
SspI AATATT 1 cut(s) 655
SspMI CTAG 5 cut(s) 695, 740, 1343, 1703, 1752
StyD4I CCNGG 4 cut(s) 142, 1452, 1741, 1742
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 6 cut(s) 19, 169, 1358, 1399, 1693, 1714
TaiI ACGT 4 cut(s) 330, 1314, 1537, 1821
TaqI TCGA 5 cut(s) 237, 1305, 1884, 1923, 2122
TaqII GACCGA 2 cut(s) 608, 1855
TatI WGTACW 1 cut(s) 360
TauI GCSGC 7 cut(s) 200, 356, 433, 482, 1118, 1181, 1983
TfiI GAWTC 4 cut(s) 231, 646, 1067, 1933
Tru1I TTAA 4 cut(s) 651, 1314, 1374, 1902
Tru9I TTAA 4 cut(s) 651, 1314, 1374, 1902
TscAI CASTG 4 cut(s) 187, 502, 1717, 1854
TseFI GTSAC 5 cut(s) 118, 491, 841, 871, 1754
Tsp45I GTSAC 5 cut(s) 118, 491, 841, 871, 1754
TspDTI ATGAA 4 cut(s) 581, 1829, 1925, 2090
TspGWI ACGGA 1 cut(s) 917
TspMI CCCGGG 1 cut(s) 1742
TspRI CASTG 4 cut(s) 187, 502, 1717, 1854
Tth111I GACNNNGTC 2 cut(s) 503, 2024
VpaK11BI GGWCC 5 cut(s) 80, 1043, 1169, 1867, 2020
XagI CCTNNNNNAGG 2 cut(s) 828, 858
XceI RCATGY 1 cut(s) 729
XcmI CCANNNNNNNNNTGG 1 cut(s) 592
XhoI CTCGAG 1 cut(s) 1883
XmaI CCCGGG 1 cut(s) 1742
XspI CTAG 5 cut(s) 695, 740, 1343, 1703, 1752
ZrmI AGTACT 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.