Rh5DG016600

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000724
Physical Location & Seq
Forward (+)
1284913 .. 1288004
3092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG016600.1

Sequence Viewer

Length: 2130 bp
ATGGAAGAAAAACGTACTTCCGAACAATCCAAACTCCTCTATCAAAGCCTGAGAAACGGTAATGCATTAGAAGTGGCGAAAGCTTATGCAAAAGCTACTACTACTGATGCAAGATCAAAGCTAATTCCAACAGTGCACGACGACACAATCCTCCATATGGCGATTTACATGGGGCAAGTAAGCATCGCAATAGGAATCATCGAAATGAGCGGTCATAATGACGAGCTAATTACAAAACGCAATGCGTTTGGAAACACAGCTCTTCATGAGGCTGCCGCAACTGATTGTGTACTTGTAGTGGAGAAGTTGTTGGAAAAGGCACCGGCATTGCTGTCCATTCCAAACGAGAAAGGGGAAATGCCTCTCTATACAGCAGCTCACTTTGGCAAGAAAAAAATTTTCATTTTGCTGGCTGAAAAACTAAAAAACAATCATCTTGATCTTCGTCCTCATTTCTACTCCCTATCTCCTTCCTACAAGTCTGTTGAAGAACTTGACGAGTTCTCCTACAGACCTGGACACATACGCAGTACCATTCTTCATGCTGCAATACATGCCGAGTTCTTTGAGCTTGCACTTTTGATAGCTAGAATGCCGGAGTATGAGAAAATTGTTCAACGAAAAGACAAAAATTCGAAGACGCCTCTTCAGCTCCTCTCATCCAATTCTTCAGCATTCAAGAGTGGAATGAAATGTGGACTGATCAAGCGGTTCATTTACAAATGTGCTCCATATGAAGATGCTCACGAACAAAATTCCAGCGATGATAGTCGTCATGTTCAGAGTTCCAAAAGATCAGCTACTCTGTTTCTTTTGGCAGTTAATAGATCGGTTTGGATTGTTCTTCGAGCATGCAGTACCATGAGGAACATCTACGACGAAAAGAAGAGGCATGGATTGGCTTTTAAACTAGCCAAGTTTCTAATTGCAGCAGACTATTCATGGGACGTCACACGACTTGGTTCAAATGAAGCCGCGAATCAGACTGCTGCTGGAGACTTCCCAATCCCAGATTTCGAAAATTCAGGTGTAGAAAATGCAGAGCCATCCCTGCCAGATAGTACTCCACCCATCCCATTGCACATTGCAACAGAGCATGGAATATTAGAGATTGTGAAGGAGACTCTTACAGCCCACCCTGCGATATTCGAGTATAGAAATACCAAGAAACAAAACATACTGCACCTGTCCATTATGCACCGTCAGCGGGACATCTTTGATCTTGTTAAAAAGAAGGAGACACCTAAGTGGAGGTTGAGACGACAAGTCGATGTAAATGACTTCACAATATTGCACCAAGTTGGCAACATAGAGCATTACAATGGAGGCACCCAGCCTGGCCCTGCGCTCCAATTGCAAGAAGAGCTGCAGTGGTTCGAGAAGGTGGAGAAGTTAATCCCACTACATTATCAAACATTCCGCGCTTGTCAGAAAGACAAAGACACCACCCTACAGTCCTTTCATAAAAGATATGTAGAGATTCTCAAGGACTTGTCTGAGAACATTGACGTGGATAAGATAAAACACGAGGACGAAAAGAAATTGGAGACCCCCGAAGAGTACTTTCGTCGAACTCATGAGCCGCTTCTCGAGAGCGCACAAGAATGGCTGAAGCGGACCTCCGAGTCGTGCTCGACGGTGGCGGGACTAATGGCCACCGTCGCCTTCGCAGCTGCCTTCACCGTCCCTGGAGGGAACGACGAGAACAGCGGCACTCCACTCCTTCTTCACAGCCCTTTCTTCTTAGTCTTCATCATCACCGACGCTCTCTCCCTAGCCAGCTCGCTGACGTCACTCGTCATGTTCCTCTCCATCCTGACGTCACCGTTCGAGCTCCACAACTTCCGCCGCTCTCTCCCTCTGCTGCTGATCCTCGGCTTCATCTTCCTCTTCTTCTCGGTGGTGGTGACCATGCTGGCCTTTGCCTCCACACTGATGCTGACAATTCCGATGAAGAACGGAGTGACCAAAAGTTTCGTGTACTGCGTTGCGTTTCTTCCCGTCACAATGTATGCGCTGTTGCAATTACCGTTGTGGGTGGCCTTCAGAAAGAGTCTCAAGTACTCAGTCCCGGTGCCCATGCAGCATCATATCTCTGCAACTAATTCGAAAACCGCCAAGACCCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

709

Amino Acids

80.23

Weight (kDa)

8.53

Isoelectric Point (pI)

50.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 51 - 140 4.5e-06 Ankyrin repeats (3 copies)
PGG PF13962 535 - 647 5.4e-24 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1624
AatII GACGTC 3 cut(s) 951, 1793, 1823
AccB1I GGYRCC 3 cut(s) 319, 1328, 2074
AccBSI CCGCTC 2 cut(s) 210, 1851
AccII CGCG 2 cut(s) 977, 1422
AclWI GGATC 1 cut(s) 1864
AcoI YGGCCR 1 cut(s) 1653
AcsI RAATTY 4 cut(s) 396, 631, 754, 1021
AcuI CTGAAG 4 cut(s) 632, 654, 1631, 2029
AcyI GRCGYC 4 cut(s) 641, 948, 1790, 1820
AfaI GTAC 8 cut(s) 16, 291, 532, 859, 1063, 1562, 1982, 2063
AfiI CCNNNNNNNGG 2 cut(s) 157, 1207
AgsI TTSAA 4 cut(s) 488, 617, 679, 966
AhdI GACNNNNNGTC 1 cut(s) 1265
AjiI CACGTC 1 cut(s) 1510
AjnI CCWGG 3 cut(s) 514, 1336, 1687
AleI CACNNNNGTG 1 cut(s) 1246
Alw21I GWGCWC 4 cut(s) 138, 730, 1634, 1836
Alw26I GTCTC 6 cut(s) 990, 1115, 1232, 1252, 1541, 2060
Alw44I GTGCAC 1 cut(s) 134
AlwI GGATC 1 cut(s) 1864
Ama87I CYCGRG 1 cut(s) 1589
AoxI GGCC 4 cut(s) 1339, 1653, 1917, 2040
ApaLI GTGCAC 1 cut(s) 134
ApoI RAATTY 4 cut(s) 396, 631, 754, 1021
AspLEI GCGC 4 cut(s) 1348, 1424, 1598, 2017
AspS9I GGNCC 2 cut(s) 1340, 1617
AsuC2I CCSGG 1 cut(s) 2072
AsuHPI GGTGA 4 cut(s) 1672, 1750, 1815, 1918
AsuII TTCGAA 3 cut(s) 635, 1017, 2108
AvaI CYCGRG 1 cut(s) 1589
AvaII GGWCC 1 cut(s) 1617
BaeGI GKGCMC 2 cut(s) 138, 2079
BalI TGGCCA 1 cut(s) 1655
BanI GGYRCC 3 cut(s) 319, 1328, 2074
BanII GRGCYC 1 cut(s) 1836
BauI CACGAG 1 cut(s) 1526
BbsI GAAGAC 2 cut(s) 644, 1741
Bbv12I GWGCWC 4 cut(s) 138, 730, 1634, 1836
BccI CCATC 3 cut(s) 1054, 1079, 1820
BcgI CGANNNNNNTGC 2 cut(s) 2088, 2122
BciT130I CCWGG 3 cut(s) 516, 1338, 1689
BclI TGATCA 1 cut(s) 702
BcnI CCSGG 1 cut(s) 2072
BcoDI GTCTC 6 cut(s) 990, 1115, 1232, 1252, 1541, 2060
BfaI CTAG 3 cut(s) 588, 911, 1775
BfmI CTRYAG 3 cut(s) 508, 1367, 1451
BmcAI AGTACT 3 cut(s) 1063, 1562, 2063
Bme1390I CCNGG 4 cut(s) 516, 1338, 1689, 2072
Bme18I GGWCC 1 cut(s) 1617
BmeRI GACNNNNNGTC 1 cut(s) 1265
BmeT110I CYCGRG 1 cut(s) 1589
BmgBI CACGTC 1 cut(s) 1510
BmgT120I GGNCC 2 cut(s) 1340, 1617
BmiI GGNNCC 3 cut(s) 321, 1330, 2076
BmrFI CCNGG 4 cut(s) 516, 1338, 1689, 2072
BmsI GCATC 5 cut(s) 97, 192, 730, 1926, 2095
BpiI GAAGAC 2 cut(s) 644, 1741
BplI GAGNNNNNCTC 2 cut(s) 1616, 1648
BpmI CTGGAG 2 cut(s) 1014, 1710
Bpu14I TTCGAA 3 cut(s) 635, 1017, 2108
BpuEI CTTGAG 2 cut(s) 1469, 2042
BpuMI CCSGG 1 cut(s) 2072
BsaHI GRCGYC 4 cut(s) 641, 948, 1790, 1820
BsaI GGTCTC 1 cut(s) 1541
BsaJI CCNNGG 2 cut(s) 1687, 1873
BsaXI ACNNNNNCTCC 6 cut(s) 293, 323, 488, 518, 1754, 1784
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 1207
Bse118I RCCGGY 1 cut(s) 322
Bse3DI GCAATG 4 cut(s) 247, 326, 1076, 1083
BseBI CCWGG 3 cut(s) 516, 1338, 1689
BseDI CCNNGG 2 cut(s) 1687, 1873
BseGI GGATG 4 cut(s) 659, 1046, 1071, 1812
BseLI CCNNNNNNNGG 2 cut(s) 157, 1207
BseMI GCAATG 4 cut(s) 247, 326, 1076, 1083
BseMII CTCAG 3 cut(s) 41, 1488, 2079
BseRI GAGGAG 2 cut(s) 26, 644
BseSI GKGCMC 2 cut(s) 138, 2079
BseYI CCCAGC 1 cut(s) 1332
BsgI GTGCAG 1 cut(s) 1166
Bsh1236I CGCG 2 cut(s) 977, 1422
BshFI GGCC 4 cut(s) 1341, 1655, 1919, 2042
BshNI GGYRCC 3 cut(s) 319, 1328, 2074
BsiHKAI GWGCWC 4 cut(s) 138, 730, 1634, 1836
BsiHKCI CYCGRG 1 cut(s) 1589
BsiSI CCGG 3 cut(s) 323, 596, 2072
BslFI GGGAC 5 cut(s) 959, 1223, 1659, 1670, 2054
BslI CCNNNNNNNGG 2 cut(s) 157, 1207
BsmAI GTCTC 6 cut(s) 990, 1115, 1232, 1252, 1541, 2060
BsmBI CGTCTC 1 cut(s) 1252
BsmFI GGGAC 5 cut(s) 959, 1223, 1659, 1670, 2054
BsmI GAATGC 2 cut(s) 597, 674
BsnI GGCC 4 cut(s) 1341, 1655, 1919, 2042
Bso31I GGTCTC 1 cut(s) 1541
BsoBI CYCGRG 1 cut(s) 1589
Bsp119I TTCGAA 3 cut(s) 635, 1017, 2108
Bsp1286I GDGCHC 5 cut(s) 138, 730, 1634, 1836, 2079
Bsp143I GATC 7 cut(s) 113, 439, 702, 794, 827, 1219, 1869
BspANI GGCC 4 cut(s) 1341, 1655, 1919, 2042
BspCNI CTCAG 3 cut(s) 42, 1489, 2078
BspFNI CGCG 2 cut(s) 977, 1422
BspHI TCATGA 2 cut(s) 265, 1576
BspLI GGNNCC 3 cut(s) 321, 1330, 2076
BspMAI CTGCAG 1 cut(s) 1371
BspPI GGATC 1 cut(s) 1864
BspQI GCTCTTC 2 cut(s) 267, 1356
BspT104I TTCGAA 3 cut(s) 635, 1017, 2108
BspT107I GGYRCC 3 cut(s) 319, 1328, 2074
BspTNI GGTCTC 1 cut(s) 1541
BsrBI CCGCTC 2 cut(s) 210, 1851
BsrDI GCAATG 4 cut(s) 247, 326, 1076, 1083
BsrFI RCCGGY 1 cut(s) 322
BssAI RCCGGY 1 cut(s) 322
BssECI CCNNGG 2 cut(s) 1687, 1873
BssMI GATC 7 cut(s) 113, 439, 702, 794, 827, 1219, 1869
BssNI GRCGYC 4 cut(s) 641, 948, 1790, 1820
BssSI CACGAG 1 cut(s) 1526
Bst2BI CACGAG 1 cut(s) 1526
Bst2UI CCWGG 3 cut(s) 516, 1338, 1689
Bst4CI ACNGT 9 cut(s) 59, 133, 1202, 1455, 1639, 1660, 1684, 1827, 2031
Bst6I CTCTTC 6 cut(s) 267, 651, 881, 1356, 1551, 1895
BstACI GRCGYC 4 cut(s) 641, 948, 1790, 1820
BstAPI GCANNNNNTGC 1 cut(s) 554
BstBI TTCGAA 3 cut(s) 635, 1017, 2108
BstC8I GCNNGC 6 cut(s) 411, 573, 853, 1780, 1784, 1917
BstDEI CTNAG 5 cut(s) 50, 1245, 1497, 1744, 2065
BstEII GGTNACC 1 cut(s) 1906
BstF5I GGATG 4 cut(s) 659, 1046, 1071, 1812
BstFNI CGCG 2 cut(s) 977, 1422
BstHHI GCGC 4 cut(s) 1348, 1424, 1598, 2017
BstKTI GATC 7 cut(s) 116, 442, 705, 797, 830, 1222, 1872
BstMAI GTCTC 6 cut(s) 990, 1115, 1232, 1252, 1541, 2060
BstMBI GATC 7 cut(s) 113, 439, 702, 794, 827, 1219, 1869
BstNI CCWGG 3 cut(s) 516, 1338, 1689
BstNSI RCATGY 2 cut(s) 557, 855
BstPI GGTNACC 1 cut(s) 1906
BstSCI CCNGG 4 cut(s) 514, 1336, 1687, 2070
BstSFI CTRYAG 3 cut(s) 508, 1367, 1451
BstSLI GKGCMC 2 cut(s) 138, 2079
BstUI CGCG 2 cut(s) 977, 1422
BstV2I GAAGAC 2 cut(s) 644, 1741
BsuRI GGCC 4 cut(s) 1341, 1655, 1919, 2042
BtgZI GCGATG 2 cut(s) 169, 777
BtrI CACGTC 1 cut(s) 1510
BtsCI GGATG 4 cut(s) 659, 1046, 1071, 1812
BtsI GCAGTG 1 cut(s) 1376
BtsIMutI CAGTG 3 cut(s) 138, 1376, 1931
Cac8I GCNNGC 6 cut(s) 411, 573, 853, 1780, 1784, 1917
CciI TCATGA 2 cut(s) 265, 1576
CfoI GCGC 4 cut(s) 1348, 1424, 1598, 2017
Cfr10I RCCGGY 1 cut(s) 322
Cfr13I GGNCC 2 cut(s) 1340, 1617
CseI GACGC 2 cut(s) 649, 1772
Csp6I GTAC 8 cut(s) 15, 290, 531, 858, 1062, 1561, 1981, 2062
CviQI GTAC 8 cut(s) 15, 290, 531, 858, 1062, 1561, 1981, 2062
DdeI CTNAG 5 cut(s) 50, 1245, 1497, 1744, 2065
DpnI GATC 7 cut(s) 115, 441, 704, 796, 829, 1221, 1871
DpnII GATC 7 cut(s) 113, 439, 702, 794, 827, 1219, 1869
DraI TTTAAA 1 cut(s) 907
DrdI GACNNNNNNGTC 1 cut(s) 1624
DriI GACNNNNNGTC 1 cut(s) 1265
DseDI GACNNNNNNGTC 1 cut(s) 1624
EaeI YGGCCR 1 cut(s) 1653
Eam1104I CTCTTC 6 cut(s) 267, 651, 881, 1356, 1551, 1895
Eam1105I GACNNNNNGTC 1 cut(s) 1265
EarI CTCTTC 6 cut(s) 267, 651, 881, 1356, 1551, 1895
EciI GGCGGA 1 cut(s) 1835
Ecl136II GAGCTC 1 cut(s) 1834
Eco24I GRGCYC 1 cut(s) 1836
Eco31I GGTCTC 1 cut(s) 1541
Eco47I GGWCC 1 cut(s) 1617
Eco53kI GAGCTC 1 cut(s) 1834
Eco57I CTGAAG 4 cut(s) 632, 654, 1631, 2029
Eco88I CYCGRG 1 cut(s) 1589
Eco91I GGTNACC 1 cut(s) 1906
EcoICRI GAGCTC 1 cut(s) 1834
EcoO65I GGTNACC 1 cut(s) 1906
EcoRII CCWGG 3 cut(s) 514, 1336, 1687
EcoT22I ATGCAT 1 cut(s) 67
EcoT38I GRGCYC 1 cut(s) 1836
Esp3I CGTCTC 1 cut(s) 1252
FaqI GGGAC 5 cut(s) 959, 1223, 1659, 1670, 2054
FauI CCCGC 2 cut(s) 1200, 1636
FauNDI CATATG 2 cut(s) 156, 733
FbaI TGATCA 1 cut(s) 702
FokI GGATG 4 cut(s) 646, 1033, 1058, 1799
FriOI GRGCYC 1 cut(s) 1836
FspBI CTAG 3 cut(s) 588, 911, 1775
GlaI GCGC 4 cut(s) 1347, 1423, 1597, 2016
GsaI CCCAGC 1 cut(s) 1336
GsuI CTGGAG 2 cut(s) 1014, 1710
HaeIII GGCC 4 cut(s) 1341, 1655, 1919, 2042
HapII CCGG 3 cut(s) 323, 596, 2072
HgaI GACGC 2 cut(s) 649, 1772
HhaI GCGC 4 cut(s) 1348, 1424, 1598, 2017
Hin1I GRCGYC 4 cut(s) 641, 948, 1790, 1820
Hin6I GCGC 4 cut(s) 1346, 1422, 1596, 2015
HinP1I GCGC 4 cut(s) 1346, 1422, 1596, 2015
HindIII AAGCTT 1 cut(s) 81
HinfI GANTC 6 cut(s) 195, 979, 1123, 1480, 1625, 2053
HpaII CCGG 3 cut(s) 323, 596, 2072
HphI GGTGA 4 cut(s) 1672, 1750, 1815, 1918
Hpy166II GTNNAC 4 cut(s) 136, 290, 698, 1981
Hpy188I TCNGA 8 cut(s) 22, 783, 984, 1431, 1498, 1624, 1950, 2048
Hpy188III TCNNGA 9 cut(s) 266, 437, 679, 746, 1378, 1577, 1589, 1591, 1816
Hpy8I GTNNAC 4 cut(s) 136, 290, 698, 1981
Hpy99I CGWCG 7 cut(s) 143, 881, 1572, 1639, 1664, 1703, 1766
HpyAV CCTTC 8 cut(s) 480, 1111, 1228, 1375, 1675, 1687, 1733, 2053
HpyCH4III ACNGT 9 cut(s) 59, 133, 1202, 1455, 1639, 1660, 1684, 1827, 2031
HpyCH4IV ACGT 5 cut(s) 13, 948, 1509, 1790, 1820
HpyF3I CTNAG 5 cut(s) 50, 1245, 1497, 1744, 2065
HpySE526I ACGT 5 cut(s) 13, 948, 1509, 1790, 1820
Hsp92I GRCGYC 4 cut(s) 641, 948, 1790, 1820
HspAI GCGC 4 cut(s) 1346, 1422, 1596, 2015
Ksp22I TGATCA 1 cut(s) 702
Kzo9I GATC 7 cut(s) 113, 439, 702, 794, 827, 1219, 1869
LguI GCTCTTC 2 cut(s) 267, 1356
LmnI GCTCC 4 cut(s) 657, 733, 1353, 1839
LweI GCATC 5 cut(s) 97, 192, 730, 1926, 2095
MaeI CTAG 3 cut(s) 588, 911, 1775
MaeII ACGT 5 cut(s) 13, 948, 1509, 1790, 1820
MaeIII GTNAC 6 cut(s) 949, 1791, 1821, 1906, 1963, 2002
MalI GATC 7 cut(s) 115, 441, 704, 796, 829, 1221, 1871
MbiI CCGCTC 2 cut(s) 210, 1851
MboI GATC 7 cut(s) 113, 439, 702, 794, 827, 1219, 1869
MfeI CAATTG 1 cut(s) 1352
MhlI GDGCHC 5 cut(s) 138, 730, 1634, 1836, 2079
MlsI TGGCCA 1 cut(s) 1655
MluNI TGGCCA 1 cut(s) 1655
MlyI GAGTC 3 cut(s) 1117, 1634, 2062
MmeI TCCRAC 2 cut(s) 152, 291
Mox20I TGGCCA 1 cut(s) 1655
Mph1103I ATGCAT 1 cut(s) 67
MscI TGGCCA 1 cut(s) 1655
MseI TTAA 4 cut(s) 822, 906, 1227, 1394
MslI CAYNNNNRTG 6 cut(s) 203, 1246, 1320, 1508, 1603, 1934
Msp20I TGGCCA 1 cut(s) 1655
MspA1I CMGCKG 3 cut(s) 1207, 1673, 1710
MspI CCGG 3 cut(s) 323, 596, 2072
MspR9I CCNGG 4 cut(s) 516, 1338, 1689, 2072
MunI CAATTG 1 cut(s) 1352
Mva1269I GAATGC 2 cut(s) 597, 674
MvaI CCWGG 3 cut(s) 516, 1338, 1689
MvnI CGCG 2 cut(s) 977, 1422
NciI CCSGG 1 cut(s) 2072
NdeI CATATG 2 cut(s) 156, 733
NdeII GATC 7 cut(s) 113, 439, 702, 794, 827, 1219, 1869
NlaIV GGNNCC 3 cut(s) 321, 1330, 2076
NmeAIII GCCGAG 2 cut(s) 583, 1854
NmuCI GTSAC 6 cut(s) 949, 1791, 1821, 1906, 1963, 2002
NsiI ATGCAT 1 cut(s) 67
NspI RCATGY 2 cut(s) 557, 855
NspV TTCGAA 3 cut(s) 635, 1017, 2108
OliI CACNNNNGTG 1 cut(s) 1246
PaeI GCATGC 1 cut(s) 855
PaeR7I CTCGAG 1 cut(s) 1589
PagI TCATGA 2 cut(s) 265, 1576
PciSI GCTCTTC 2 cut(s) 267, 1356
PcsI WCGNNNNNNNCGW 1 cut(s) 1983
PctI GAATGC 2 cut(s) 597, 674
PfeI GAWTC 3 cut(s) 195, 979, 1480
PleI GAGTC 3 cut(s) 1117, 1633, 2061
PpsI GAGTC 3 cut(s) 1117, 1633, 2061
Psp124BI GAGCTC 1 cut(s) 1836
Psp6I CCWGG 3 cut(s) 514, 1336, 1687
PspEI GGTNACC 1 cut(s) 1906
PspFI CCCAGC 1 cut(s) 1332
PspGI CCWGG 3 cut(s) 514, 1336, 1687
PspN4I GGNNCC 3 cut(s) 321, 1330, 2076
PspPI GGNCC 2 cut(s) 1340, 1617
PstI CTGCAG 1 cut(s) 1371
PvuII CAGCTG 1 cut(s) 1673
RsaI GTAC 8 cut(s) 16, 291, 532, 859, 1063, 1562, 1982, 2063
RsaNI GTAC 8 cut(s) 15, 290, 531, 858, 1062, 1561, 1981, 2062
RseI CAYNNNNRTG 6 cut(s) 203, 1246, 1320, 1508, 1603, 1934
SacI GAGCTC 1 cut(s) 1836
SapI GCTCTTC 2 cut(s) 267, 1356
SaqAI TTAA 4 cut(s) 822, 906, 1227, 1394
Sau3AI GATC 7 cut(s) 113, 439, 702, 794, 827, 1219, 1869
Sau96I GGNCC 2 cut(s) 1340, 1617
ScaI AGTACT 3 cut(s) 1063, 1562, 2063
SchI GAGTC 3 cut(s) 1117, 1634, 2062
ScrFI CCNGG 4 cut(s) 516, 1338, 1689, 2072
SduI GDGCHC 5 cut(s) 138, 730, 1634, 1836, 2079
SfaNI GCATC 5 cut(s) 97, 192, 730, 1926, 2095
SfcI CTRYAG 3 cut(s) 508, 1367, 1451
Sfr274I CTCGAG 1 cut(s) 1589
SfuI TTCGAA 3 cut(s) 635, 1017, 2108
SinI GGWCC 1 cut(s) 1617
SlaI CTCGAG 1 cut(s) 1589
SmiMI CAYNNNNRTG 6 cut(s) 203, 1246, 1320, 1508, 1603, 1934
SmlI CTYRAG 3 cut(s) 1484, 1589, 2057
SmoI CTYRAG 3 cut(s) 1484, 1589, 2057
SphI GCATGC 1 cut(s) 855
SspI AATATT 2 cut(s) 1104, 1290
SspMI CTAG 3 cut(s) 588, 911, 1775
SstI GAGCTC 1 cut(s) 1836
StyD4I CCNGG 4 cut(s) 514, 1336, 1687, 2070
TaaI ACNGT 9 cut(s) 59, 133, 1202, 1455, 1639, 1660, 1684, 1827, 2031
TaiI ACGT 5 cut(s) 16, 951, 1512, 1793, 1823
TatI WGTACW 5 cut(s) 289, 1061, 1560, 1980, 2061
TauI GCSGC 5 cut(s) 278, 977, 1585, 1713, 1851
TfiI GAWTC 3 cut(s) 195, 979, 1480
Tru1I TTAA 4 cut(s) 822, 906, 1227, 1394
Tru9I TTAA 4 cut(s) 822, 906, 1227, 1394
TscAI CASTG 3 cut(s) 138, 1376, 1938
TseFI GTSAC 6 cut(s) 949, 1791, 1821, 1906, 1963, 2002
Tsp45I GTSAC 6 cut(s) 949, 1791, 1821, 1906, 1963, 2002
TspGWI ACGGA 1 cut(s) 1974
TspRI CASTG 3 cut(s) 138, 1376, 1938
VneI GTGCAC 1 cut(s) 134
VpaK11BI GGWCC 1 cut(s) 1617
XapI RAATTY 4 cut(s) 396, 631, 754, 1021
XceI RCATGY 2 cut(s) 557, 855
XhoI CTCGAG 1 cut(s) 1589
XspI CTAG 3 cut(s) 588, 911, 1775
ZraI GACGTC 3 cut(s) 949, 1791, 1821
ZrmI AGTACT 3 cut(s) 1063, 1562, 2063
Zsp2I ATGCAT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.