Rroxscaffold_1G00074460

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000724
Physical Location & Seq
Reverse (-)
95452595 .. 95456204
3610 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00074460.1

Sequence Viewer

Length: 933 bp
ATGGAAGGAAAACGTACTTCCGAACAATTCCTGCTCTATCAAAGCCTCAGAGACGGTAATGTATTAGAAGTGGTGAAAGCTTGTGCAAAAGCTACTACTCTTGAAGCAAGATTGAAGCTAATTCCAACAGTGCACGACGACACAATCCTCCATATCGCGATTTACATGAGGCAAGAAAGCATCGCAATGAAAATCATCGAAATTAGCAGCGGGGATAATGACGAGCTAATCACAAAACGCAATCAGTTTGGAAACACAGCTCTTCATGAGGCTGCAGCAACTGATTTTGTACGTGTTGTGGAGAAGTTGTTGGAAAAGGCACCGGCATTGCTGTCCATTCCAAACAAGAAAGGGGAAATGCCTCTCTATACAGCAGCTCACTTTGGCCAGAAAAAAATTTTCATTTTGCTGGCTGAAAAACTGAAAAACAATCATTCTGATCTTCGTCCTCATTTATGCTCCCTATCTCCTTCCTACAAGTCTGTTGAAGAACTTGACGAGCTCTCCTACGGCCCTGGACACATACGCAGTACCATTCTTCATGCTGCAATACATGCCGAGTTCTTTGAGCTTGCACTTTTGATAGCTAAAAAGCCGGAGTATGAGAAAATTGTTCAACTAAAAGACAAAAATTCGAAGACACCTCTTCAGCTCCTCTCATCCAATTCTTCAGCATTCAAGAGTGGATTGAAATGTGGACTGATCAAGAGGTTCATTTACAACTGTGCTCCATATGAGAATGCTAATGAAGAAGATGCTCATGAACAAAATTCCTGTAATGATAGTCATGTACAAAGTTCCAGGAAAGCTACTCCGTTTCTATGGCGAGTCATCTCTTCAGTTAATAGATCGGTTAATTTGGATTGTTCTTCGAGAATGGAGTACCATGAGGGACATCTACGAAGAAAGGAAGAGGCATGGATTGGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

310

Amino Acids

35.05

Weight (kDa)

8.13

Isoelectric Point (pI)

47.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 79 - 140 6.5e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 319
AccII CGCG 1 cut(s) 158
AciI CCGC 1 cut(s) 210
AcoI YGGCCR 1 cut(s) 385
AcsI RAATTY 3 cut(s) 396, 631, 769
AcuI CTGAAG 3 cut(s) 632, 654, 822
AfaI GTAC 5 cut(s) 16, 291, 532, 792, 884
AflIII ACRYGT 1 cut(s) 292
AgsI TTSAA 6 cut(s) 104, 115, 488, 617, 679, 691
AjnI CCWGG 2 cut(s) 514, 800
Alw21I GWGCWC 3 cut(s) 135, 504, 730
Alw26I GTCTC 1 cut(s) 45
Alw44I GTGCAC 1 cut(s) 131
AlwNI CAGNNNCTG 1 cut(s) 281
AoxI GGCC 2 cut(s) 385, 511
ApaLI GTGCAC 1 cut(s) 131
ApeKI GCWGC 5 cut(s) 207, 272, 275, 374, 545
ApoI RAATTY 3 cut(s) 396, 631, 769
AspS9I GGNCC 1 cut(s) 512
AsuHPI GGTGA 1 cut(s) 85
AsuII TTCGAA 1 cut(s) 635
BaeGI GKGCMC 1 cut(s) 135
BalI TGGCCA 1 cut(s) 387
BanI GGYRCC 1 cut(s) 319
BanII GRGCYC 1 cut(s) 504
BbsI GAAGAC 1 cut(s) 644
Bbv12I GWGCWC 3 cut(s) 135, 504, 730
BbvI GCAGC 5 cut(s) 219, 259, 287, 386, 532
BceAI ACGGC 1 cut(s) 526
BciT130I CCWGG 2 cut(s) 516, 802
BclI TGATCA 1 cut(s) 702
BcoDI GTCTC 1 cut(s) 45
BfmI CTRYAG 1 cut(s) 273
BisI GCNGC 5 cut(s) 208, 273, 276, 375, 546
BlsI GCNGC 5 cut(s) 209, 274, 277, 376, 547
Bme1390I CCNGG 2 cut(s) 516, 802
BmgT120I GGNCC 1 cut(s) 512
BmiI GGNNCC 1 cut(s) 321
BmrFI CCNGG 2 cut(s) 516, 802
BmsI GCATC 2 cut(s) 189, 745
BpiI GAAGAC 1 cut(s) 644
Bpu14I TTCGAA 1 cut(s) 635
BsaAI YACGTR 1 cut(s) 293
BsaJI CCNNGG 1 cut(s) 514
BsaXI ACNNNNNCTCC 4 cut(s) 293, 323, 488, 518
Bse118I RCCGGY 1 cut(s) 322
Bse3DI GCAATG 2 cut(s) 192, 326
BseBI CCWGG 2 cut(s) 516, 802
BseDI CCNNGG 1 cut(s) 514
BseGI GGATG 1 cut(s) 659
BseMI GCAATG 2 cut(s) 192, 326
BseMII CTCAG 1 cut(s) 61
BseRI GAGGAG 1 cut(s) 644
BseSI GKGCMC 1 cut(s) 135
BseXI GCAGC 5 cut(s) 219, 259, 287, 386, 532
Bsh1236I CGCG 1 cut(s) 158
BshFI GGCC 2 cut(s) 387, 513
BshNI GGYRCC 1 cut(s) 319
BsiHKAI GWGCWC 3 cut(s) 135, 504, 730
BsiSI CCGG 2 cut(s) 323, 596
BslFI GGGAC 1 cut(s) 906
BsmAI GTCTC 1 cut(s) 45
BsmBI CGTCTC 1 cut(s) 45
BsmFI GGGAC 1 cut(s) 906
BsmI GAATGC 2 cut(s) 674, 745
BsnI GGCC 2 cut(s) 387, 513
Bsp119I TTCGAA 1 cut(s) 635
Bsp1286I GDGCHC 3 cut(s) 135, 504, 730
Bsp1407I TGTACA 1 cut(s) 790
Bsp143I GATC 3 cut(s) 439, 702, 848
Bsp68I TCGCGA 1 cut(s) 158
BspACI CCGC 1 cut(s) 210
BspANI GGCC 2 cut(s) 387, 513
BspCNI CTCAG 1 cut(s) 60
BspFNI CGCG 1 cut(s) 158
BspHI TCATGA 2 cut(s) 265, 760
BspLI GGNNCC 1 cut(s) 321
BspMAI CTGCAG 1 cut(s) 277
BspQI GCTCTTC 1 cut(s) 267
BspT104I TTCGAA 1 cut(s) 635
BspT107I GGYRCC 1 cut(s) 319
BsrDI GCAATG 2 cut(s) 192, 326
BsrFI RCCGGY 1 cut(s) 322
BsrGI TGTACA 1 cut(s) 790
BssAI RCCGGY 1 cut(s) 322
BssECI CCNNGG 1 cut(s) 514
BssMI GATC 3 cut(s) 439, 702, 848
Bst2UI CCWGG 2 cut(s) 516, 802
Bst4CI ACNGT 3 cut(s) 56, 130, 725
Bst6I CTCTTC 4 cut(s) 267, 651, 841, 906
BstAPI GCANNNNNTGC 1 cut(s) 554
BstAUI TGTACA 1 cut(s) 790
BstBAI YACGTR 1 cut(s) 293
BstBI TTCGAA 1 cut(s) 635
BstC8I GCNNGC 2 cut(s) 411, 573
BstDEI CTNAG 1 cut(s) 47
BstF5I GGATG 1 cut(s) 659
BstFNI CGCG 1 cut(s) 158
BstKTI GATC 3 cut(s) 442, 705, 851
BstMAI GTCTC 1 cut(s) 45
BstMBI GATC 3 cut(s) 439, 702, 848
BstMWI GCNNNNNNNGC 1 cut(s) 554
BstNI CCWGG 2 cut(s) 516, 802
BstNSI RCATGY 1 cut(s) 557
BstSCI CCNGG 2 cut(s) 514, 800
BstSFI CTRYAG 1 cut(s) 273
BstSLI GKGCMC 1 cut(s) 135
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 5 cut(s) 219, 259, 287, 386, 532
BstV2I GAAGAC 1 cut(s) 644
BsuRI GGCC 2 cut(s) 387, 513
BtgZI GCGATG 1 cut(s) 166
BtsCI GGATG 1 cut(s) 659
BtsIMutI CAGTG 1 cut(s) 135
BtuMI TCGCGA 1 cut(s) 158
Cac8I GCNNGC 2 cut(s) 411, 573
CaiI CAGNNNCTG 1 cut(s) 281
CciI TCATGA 2 cut(s) 265, 760
Cfr10I RCCGGY 1 cut(s) 322
Cfr13I GGNCC 1 cut(s) 512
Csp6I GTAC 5 cut(s) 15, 290, 531, 791, 883
CviAII CATG 8 cut(s) 166, 266, 542, 554, 761, 788, 887, 918
CviQI GTAC 5 cut(s) 15, 290, 531, 791, 883
DdeI CTNAG 1 cut(s) 47
DpnI GATC 3 cut(s) 441, 704, 850
DpnII GATC 3 cut(s) 439, 702, 848
EaeI YGGCCR 1 cut(s) 385
Eam1104I CTCTTC 4 cut(s) 267, 651, 841, 906
EarI CTCTTC 4 cut(s) 267, 651, 841, 906
Ecl136II GAGCTC 1 cut(s) 502
Eco24I GRGCYC 1 cut(s) 504
Eco53kI GAGCTC 1 cut(s) 502
Eco57I CTGAAG 3 cut(s) 632, 654, 822
EcoICRI GAGCTC 1 cut(s) 502
EcoRII CCWGG 2 cut(s) 514, 800
EcoT38I GRGCYC 1 cut(s) 504
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 8 cut(s) 169, 269, 545, 557, 764, 791, 890, 921
FaqI GGGAC 1 cut(s) 906
FatI CATG 8 cut(s) 165, 265, 541, 553, 760, 787, 886, 917
FauI CCCGC 1 cut(s) 203
FauNDI CATATG 1 cut(s) 733
FbaI TGATCA 1 cut(s) 702
Fnu4HI GCNGC 5 cut(s) 208, 273, 276, 375, 546
FokI GGATG 1 cut(s) 646
FriOI GRGCYC 1 cut(s) 504
Fsp4HI GCNGC 5 cut(s) 208, 273, 276, 375, 546
GluI GCNGC 5 cut(s) 208, 273, 276, 375, 546
HaeIII GGCC 2 cut(s) 387, 513
HapII CCGG 2 cut(s) 323, 596
Hin1II CATG 8 cut(s) 169, 269, 545, 557, 764, 791, 890, 921
HindIII AAGCTT 1 cut(s) 78
HinfI GANTC 1 cut(s) 828
HpaII CCGG 2 cut(s) 323, 596
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 2 cut(s) 133, 698
Hpy188I TCNGA 3 cut(s) 22, 50, 439
Hpy188III TCNNGA 7 cut(s) 101, 157, 266, 679, 706, 761, 873
Hpy8I GTNNAC 2 cut(s) 133, 698
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 1 cut(s) 480
HpyCH4III ACNGT 3 cut(s) 56, 130, 725
HpyCH4IV ACGT 2 cut(s) 13, 292
HpyCH4V TGCA 5 cut(s) 86, 133, 275, 548, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 554
HpyF3I CTNAG 1 cut(s) 47
HpySE526I ACGT 2 cut(s) 13, 292
Hsp92II CATG 8 cut(s) 169, 269, 545, 557, 764, 791, 890, 921
Ksp22I TGATCA 1 cut(s) 702
Kzo9I GATC 3 cut(s) 439, 702, 848
LguI GCTCTTC 1 cut(s) 267
LmnI GCTCC 3 cut(s) 464, 657, 733
Lsp1109I GCAGC 5 cut(s) 219, 259, 287, 386, 532
LweI GCATC 2 cut(s) 189, 745
MaeII ACGT 2 cut(s) 13, 292
MalI GATC 3 cut(s) 441, 704, 850
MboI GATC 3 cut(s) 439, 702, 848
MhlI GDGCHC 3 cut(s) 135, 504, 730
MlsI TGGCCA 1 cut(s) 387
MluCI AATT 9 cut(s) 26, 120, 201, 396, 609, 631, 664, 769, 856
MluNI TGGCCA 1 cut(s) 387
MlyI GAGTC 1 cut(s) 837
MmeI TCCRAC 2 cut(s) 149, 291
Mox20I TGGCCA 1 cut(s) 387
MscI TGGCCA 1 cut(s) 387
MseI TTAA 2 cut(s) 843, 855
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 1 cut(s) 387
MspA1I CMGCKG 1 cut(s) 210
MspI CCGG 2 cut(s) 323, 596
MspR9I CCNGG 2 cut(s) 516, 802
Mva1269I GAATGC 2 cut(s) 674, 745
MvaI CCWGG 2 cut(s) 516, 802
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 1 cut(s) 554
NdeI CATATG 1 cut(s) 733
NdeII GATC 3 cut(s) 439, 702, 848
NlaIII CATG 8 cut(s) 169, 269, 545, 557, 764, 791, 890, 921
NlaIV GGNNCC 1 cut(s) 321
NmeAIII GCCGAG 1 cut(s) 583
NruI TCGCGA 1 cut(s) 158
NspI RCATGY 1 cut(s) 557
NspV TTCGAA 1 cut(s) 635
PagI TCATGA 2 cut(s) 265, 760
PciSI GCTCTTC 1 cut(s) 267
PctI GAATGC 2 cut(s) 674, 745
PfoI TCCNGGA 1 cut(s) 800
PkrI GCNGC 5 cut(s) 209, 274, 277, 376, 547
PleI GAGTC 1 cut(s) 836
PpsI GAGTC 1 cut(s) 836
Ppu21I YACGTR 1 cut(s) 293
Psp124BI GAGCTC 1 cut(s) 504
Psp6I CCWGG 2 cut(s) 514, 800
PspGI CCWGG 2 cut(s) 514, 800
PspN4I GGNNCC 1 cut(s) 321
PspPI GGNCC 1 cut(s) 512
PstI CTGCAG 1 cut(s) 277
PstNI CAGNNNCTG 1 cut(s) 281
RruI TCGCGA 1 cut(s) 158
RsaI GTAC 5 cut(s) 16, 291, 532, 792, 884
RsaNI GTAC 5 cut(s) 15, 290, 531, 791, 883
RseI CAYNNNNRTG 1 cut(s) 185
SacI GAGCTC 1 cut(s) 504
SapI GCTCTTC 1 cut(s) 267
SaqAI TTAA 2 cut(s) 843, 855
SatI GCNGC 5 cut(s) 208, 273, 276, 375, 546
Sau3AI GATC 3 cut(s) 439, 702, 848
Sau96I GGNCC 1 cut(s) 512
SchI GAGTC 1 cut(s) 837
ScrFI CCNGG 2 cut(s) 516, 802
SduI GDGCHC 3 cut(s) 135, 504, 730
SfaNI GCATC 2 cut(s) 189, 745
SfcI CTRYAG 1 cut(s) 273
SfuI TTCGAA 1 cut(s) 635
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 9 cut(s) 26, 120, 201, 396, 609, 631, 664, 769, 856
SsiI CCGC 1 cut(s) 210
SstI GAGCTC 1 cut(s) 504
StyD4I CCNGG 2 cut(s) 514, 800
TaaI ACNGT 3 cut(s) 56, 130, 725
TaiI ACGT 2 cut(s) 16, 295
TaqI TCGA 3 cut(s) 198, 635, 872
TasI AATT 9 cut(s) 26, 120, 201, 396, 609, 631, 664, 769, 856
TatI WGTACW 1 cut(s) 790
Tru1I TTAA 2 cut(s) 843, 855
Tru9I TTAA 2 cut(s) 843, 855
TscAI CASTG 1 cut(s) 135
TseI GCWGC 5 cut(s) 207, 272, 275, 374, 545
TspDTI ATGAA 7 cut(s) 203, 254, 391, 530, 703, 762, 777
TspGWI ACGGA 1 cut(s) 804
TspRI CASTG 1 cut(s) 135
VneI GTGCAC 1 cut(s) 131
XapI RAATTY 3 cut(s) 396, 631, 769
XceI RCATGY 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.