MD05G1353100.v1.1

Ankyrin repeat-containing protein At3g12360-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000724
Physical Location & Seq
Reverse (-)
46944597 .. 46947930
3334 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1353100.v1.1.491

Sequence Viewer

Length: 2394 bp
ATGGAGTACTGGAAAACGCTAACGGTTCGGTCGGATCTGATGAAAGAGGGAGAGGATAGCTCTCGGCCGGATCAGAAAGAGAGAGAGAGATTAAAGAAAATGGAAAAGTTGAAAGATTCCTATAAATATGCCATGGAACAGAAATGTGAAGAGCTGAAGGAAATGTTCGAGAATCATCCGGAAATAATGCGCGAAGAAATGACAGTGGACGGGGACACTGTATTACACCTTGCGGCTTCTTACAGCAGCAAATCACAAGGTAAAAAAGTCCTCGAAATGCTTATCAACATACTCTGTTCAATCAATGACAAGATGAAAGCTGTGAGGGTTCCAAATAAGTACGGAAACAATACTCTCCACGAGGTGAGTGTGTCCGGCAATAAGGAAGCGGCAGAGTACCTGGTGAGCAACTTCAACAAGCCTGTTCCAGAAGAGGTCAAGATCAGTACTAGTAGTACTGATAAAGAGAATTATGCGCCGCAGCTGCGGGAGGCTCGGGATTTAGAGAATTATGAGCTGCTGCTGCGGGAGGCTCGGGATTTAGAGAATGAGCTGCAGAAGCAGAAGGCTCGGGATTCAGAGAATTATGCGCTGCAGCTGCTGGAGACTCGGAACTATTTAGGAGAAACTCCGCTCTTCAGGGCAGCTGCTCTCGGTCACACGGACTTGGTCAAGCTTTATGCCGACAAACTACAGGCGGACAATCTTTGGAGGCACTTCCACAGAGATGACAAAATGTCCGTTCTTCATATCACAGTCGTTGCGCAACATTTTGAGACTGCTATTTGGTTATTGGAGAAATACCCATCTCTAGCGCTCCTTGAGGAAGATAAAGGATTGACAAGCCTTCAGTTGCTAGCCCAAATGCCAACTGCCTTTTATCCAAATTTTGAAGAAAGCAAATGGAAGATGCTAATTTATTATTGCCTTCCTGATGGAGGAAATGTGGTCACACCACATCAGAAGGATGATGTGGAGAGCGGCATGGATGGCAACCATCCCCGTCCATCCGTCTTTCGGAAAAAGCTTGCAGTTGTTATGAAGGCTTACTATTCATTATGGGACTCCCTTGCTAAAGGAGGCCCACAGAAAGAGGTAATCTACATGATATGGAACGAGAAGAAAAACAAAAAATTTCTGAAGAAACTCATCCAATTGCTCGTCAAGTGGGACGACCATTCTTTGTTGACTCCTAGTAAGCAAGCAAAAATCAGGACCATTTCTCTATGGGAAAAACCTGATAAAGAAAAGGAAGCAACCAAATCCGGTACGACAAATGGTGATGAAGGTGGTGAAAAAGGAAACGCGGCCGCAGATAAGCATTTCTCTTGGGTCAGGGAGATAACCGAATTCGATAGATATGAAAAAGAAAAGGAAGCAACCAAATCGAGAAAGGAGGCGAGGAGTGAATTATACAACTCTTCCCCATTGCTTATAGCAACTATCAAAGGAATTGAACCCATTGTACGGGAGATACTTGAGCAACGTCCTCAGGCAGCCGAGCATGTTAACCGTTACGAACGCAACATTTTGCATCTGGCCATTAAGAACCGTAGGGAAAATATCCTTGATCTTATCGAAAGCGAACCAACTCTGATGTCAAAGCTGAGTATGAGGATAGACCGCAATGGAAACACCATATTGCACCAGGCTGCAGATAGAACTTACTACTCTATAGCAGTGGCTCAGAAAGTAATAGGCCCTGCCATGGAATTGCAAAAAGAGCTGCTCTGGAAGCAGCGTATAAAAGAGATGTTACCACCTCATTACATCATGCACCACAACGACAACGATCAGACAGAGGAGGAGTTGTTCAACGCTGGGCATGACGAGCTTCTGAAGTCCGCACAAGATTGGGTAAAAGAAACGGCTCGGTCATGCTCAACCGTGGCGGTGCTAGTGGCCACTGTGGTCTTTGCAGCCGCCTACGCCATTCCTGGGGGTAACGAGCAAAATGGTCGTCCTGTTTTCGAGAACAATCCTCTCTTTTGGCTCTTTACCAGCATGGACGTTGTGGCCATCGCCTGCTCATTATCTTCTGTGGCATTCTTTCTCTCGGTCCTCTCCTCCCCTCTCGAGTACCCAAATTTCGTTAAAAGCATTCCTCACAAGGTCATGATAGGATTCATCTTTCTCTTCTTGTCTATGGCAACCACCATGCTCGCCTTTGCTGCCACTATTTTGCTCTTGATTCGCATCGAGAAAAAATGGACCAAGTCTCTATTTTACCCTATTGCCTTTTTCCCAGTCCCTCTATTCGGCCTGCTTCAGTTTCCTGTGTATCAATTTGTCAAAACCATGTTTCTGAAAATGGATGCCTGGTTTTTCAAACCCTTACTCCGCTCCCTTGGCTTTCGCAAGAAGAAATTAATTTGGAAGACCAAGGCGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

798

Amino Acids

92.04

Weight (kDa)

8.47

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 618 - 728 3.2e-21 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 765
AccBSI CCGCTC 3 cut(s) 634, 981, 2343
AccII CGCG 2 cut(s) 192, 1307
AccIII TCCGGA 1 cut(s) 178
AclWI GGATC 2 cut(s) 42, 78
AcoI YGGCCR 5 cut(s) 65, 1308, 1539, 1902, 2016
AcsI RAATTY 4 cut(s) 886, 1133, 1349, 2086
AcuI CTGAAG 6 cut(s) 176, 622, 833, 1160, 1859, 2252
AdeI CACNNNGTG 1 cut(s) 364
AfaI GTAC 8 cut(s) 8, 341, 398, 448, 457, 1270, 1467, 2081
AfeI AGCGCT 1 cut(s) 816
AfiI CCNNNNNNNGG 6 cut(s) 938, 1017, 1467, 1708, 1938, 2258
AgsI TTSAA 7 cut(s) 112, 300, 415, 893, 1457, 1816, 2329
AhdI GACNNNNNGTC 1 cut(s) 736
AhlI ACTAGT 1 cut(s) 449
AjnI CCWGG 4 cut(s) 399, 1647, 1936, 2318
AloI GAACNNNNNNTCC 2 cut(s) 1796, 1828
Alw26I GTCTC 3 cut(s) 599, 770, 2223
AlwI GGATC 2 cut(s) 42, 78
AlwNI CAGNNNCTG 1 cut(s) 601
Ama87I CYCGRG 4 cut(s) 495, 534, 570, 2075
Aor13HI TCCGGA 1 cut(s) 178
Aor51HI AGCGCT 1 cut(s) 816
AoxI GGCC 8 cut(s) 65, 1081, 1308, 1539, 1699, 1902, 2016, 2260
ApoI RAATTY 4 cut(s) 886, 1133, 1349, 2086
ArsI GACNNNNNNTTYG 2 cut(s) 1945, 1977
AseI ATTAAT 1 cut(s) 2369
AspLEI GCGC 6 cut(s) 192, 478, 592, 766, 817, 2389
AspS9I GGNCC 5 cut(s) 1082, 1215, 1700, 2059, 2211
AsuHPI GGTGA 4 cut(s) 376, 415, 1292, 1304
AsuNHI GCTAGC 1 cut(s) 856
AvaI CYCGRG 4 cut(s) 495, 534, 570, 2075
AvaII GGWCC 3 cut(s) 1215, 2059, 2211
AxyI CCTNAGG 1 cut(s) 1491
BalI TGGCCA 3 cut(s) 1541, 1904, 2018
BauI CACGAG 1 cut(s) 359
BbsI GAAGAC 1 cut(s) 2384
BccI CCATC 6 cut(s) 814, 929, 983, 1005, 1015, 2027
BceAI ACGGC 1 cut(s) 1884
BcgI CGANNNNNNTGC 6 cut(s) 551, 585, 1368, 1402, 1940, 1974
BciT130I CCWGG 4 cut(s) 401, 1649, 1938, 2320
BcoDI GTCTC 3 cut(s) 599, 770, 2223
BcuI ACTAGT 1 cut(s) 449
BfaI CTAG 5 cut(s) 450, 812, 857, 1194, 1898
BfmI CTRYAG 5 cut(s) 554, 593, 692, 1653, 1674
BfoI RGCGCY 1 cut(s) 818
BmcAI AGTACT 3 cut(s) 8, 448, 457
Bme1390I CCNGG 4 cut(s) 401, 1649, 1938, 2320
Bme18I GGWCC 3 cut(s) 1215, 2059, 2211
BmeRI GACNNNNNGTC 1 cut(s) 736
BmeT110I CYCGRG 4 cut(s) 495, 534, 570, 2075
BmgT120I GGNCC 5 cut(s) 1082, 1215, 1700, 2059, 2211
BmiI GGNNCC 1 cut(s) 330
BmrFI CCNGG 4 cut(s) 401, 1649, 1938, 2320
BmrI ACTGGG 1 cut(s) 2240
BmsI GCATC 4 cut(s) 900, 1543, 2205, 2305
BmtI GCTAGC 1 cut(s) 860
BmuI ACTGGG 1 cut(s) 2240
BpiI GAAGAC 1 cut(s) 2384
BplI GAGNNNNNCTC 2 cut(s) 44, 76
BpmI CTGGAG 1 cut(s) 623
BpuEI CTTGAG 2 cut(s) 842, 1499
BsaBI GATNNNNATC 1 cut(s) 2195
BsaJI CCNNGG 6 cut(s) 132, 1707, 1887, 1937, 2347, 2382
BsaWI WCCGGW 2 cut(s) 178, 1265
BsaXI ACNNNNNCTCC 6 cut(s) 930, 960, 1796, 1826, 2322, 2352
Bsc4I CCNNNNNNNGG 6 cut(s) 938, 1017, 1467, 1708, 1938, 2258
Bse1I ACTGG 2 cut(s) 14, 2246
Bse21I CCTNAGG 1 cut(s) 1491
Bse3DI GCAATG 2 cut(s) 1427, 1633
Bse8I GATNNNNATC 1 cut(s) 2195
BseAI TCCGGA 1 cut(s) 178
BseBI CCWGG 4 cut(s) 401, 1649, 1938, 2320
BseDI CCNNGG 6 cut(s) 132, 1707, 1887, 1937, 2347, 2382
BseGI GGATG 7 cut(s) 175, 973, 994, 997, 1007, 1149, 2320
BseJI GATNNNNATC 1 cut(s) 2195
BseLI CCNNNNNNNGG 6 cut(s) 938, 1017, 1467, 1708, 1938, 2258
BseMI GCAATG 2 cut(s) 1427, 1633
BseMII CTCAG 3 cut(s) 1505, 1598, 1700
BseNI ACTGG 2 cut(s) 14, 2246
BseRI GAGGAG 4 cut(s) 1417, 1817, 1820, 2056
BseX3I CGGCCG 2 cut(s) 65, 1308
BseYI CCCAGC 1 cut(s) 1820
Bsh1236I CGCG 2 cut(s) 192, 1307
Bsh1285I CGRYCG 3 cut(s) 32, 68, 1311
BshFI GGCC 8 cut(s) 67, 1083, 1310, 1541, 1701, 1904, 2018, 2262
BsiEI CGRYCG 3 cut(s) 32, 68, 1311
BsiHKCI CYCGRG 4 cut(s) 495, 534, 570, 2075
BsiSI CCGG 4 cut(s) 68, 179, 375, 1266
BslFI GGGAC 4 cut(s) 227, 1076, 1184, 2234
BslI CCNNNNNNNGG 6 cut(s) 938, 1017, 1467, 1708, 1938, 2258
BsmAI GTCTC 3 cut(s) 599, 770, 2223
BsmFI GGGAC 4 cut(s) 227, 1076, 1184, 2234
BsmI GAATGC 2 cut(s) 2045, 2100
BsnI GGCC 8 cut(s) 67, 1083, 1310, 1541, 1701, 1904, 2018, 2262
BsoBI CYCGRG 4 cut(s) 495, 534, 570, 2075
Bsp13I TCCGGA 1 cut(s) 178
Bsp143I GATC 5 cut(s) 34, 70, 441, 1570, 1792
Bsp19I CCATGG 2 cut(s) 132, 1707
BspANI GGCC 8 cut(s) 67, 1083, 1310, 1541, 1701, 1904, 2018, 2262
BspCNI CTCAG 3 cut(s) 1504, 1599, 1699
BspEI TCCGGA 1 cut(s) 178
BspFNI CGCG 2 cut(s) 192, 1307
BspHI TCATGA 1 cut(s) 2115
BspLI GGNNCC 1 cut(s) 330
BspMAI CTGCAG 3 cut(s) 558, 597, 1657
BspOI GCTAGC 1 cut(s) 860
BspPI GGATC 2 cut(s) 42, 78
BspQI GCTCTTC 2 cut(s) 144, 641
BsrBI CCGCTC 3 cut(s) 634, 981, 2343
BsrDI GCAATG 2 cut(s) 1427, 1633
BsrI ACTGG 2 cut(s) 14, 2246
BssECI CCNNGG 6 cut(s) 132, 1707, 1887, 1937, 2347, 2382
BssMI GATC 5 cut(s) 34, 70, 441, 1570, 1792
BssSI CACGAG 1 cut(s) 359
BssT1I CCWWGG 4 cut(s) 132, 1707, 2347, 2382
Bst2BI CACGAG 1 cut(s) 359
Bst2UI CCWGG 4 cut(s) 401, 1649, 1938, 2320
Bst4CI ACNGT 8 cut(s) 25, 205, 220, 757, 1514, 1553, 1888, 1909
Bst6I CTCTTC 5 cut(s) 144, 426, 641, 1426, 2141
BstC8I GCNNGC 6 cut(s) 858, 1029, 1203, 2026, 2163, 2264
BstDEI CTNAG 3 cut(s) 1491, 1607, 1686
BstDSI CCRYGG 3 cut(s) 132, 1707, 1887
BstENI CCTNNNNNAGG 1 cut(s) 936
BstF5I GGATG 7 cut(s) 175, 973, 994, 997, 1007, 1149, 2320
BstFNI CGCG 2 cut(s) 192, 1307
BstH2I RGCGCY 1 cut(s) 818
BstHHI GCGC 6 cut(s) 192, 478, 592, 766, 817, 2389
BstKTI GATC 5 cut(s) 37, 73, 444, 1573, 1795
BstMAI GTCTC 3 cut(s) 599, 770, 2223
BstMBI GATC 5 cut(s) 34, 70, 441, 1570, 1792
BstMCI CGRYCG 3 cut(s) 32, 68, 1311
BstNI CCWGG 4 cut(s) 401, 1649, 1938, 2320
BstNSI RCATGY 1 cut(s) 1508
BstSCI CCNGG 4 cut(s) 399, 1647, 1936, 2318
BstSFI CTRYAG 5 cut(s) 554, 593, 692, 1653, 1674
BstUI CGCG 2 cut(s) 192, 1307
BstV2I GAAGAC 1 cut(s) 2384
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
BstZI CGGCCG 2 cut(s) 65, 1308
Bsu36I CCTNAGG 1 cut(s) 1491
BsuRI GGCC 8 cut(s) 67, 1083, 1310, 1541, 1701, 1904, 2018, 2262
BtgI CCRYGG 3 cut(s) 132, 1707, 1887
BtgZI GCGATG 1 cut(s) 2005
BtsCI GGATG 7 cut(s) 175, 973, 994, 997, 1007, 1149, 2320
BtsI GCAGTG 1 cut(s) 1686
BtsIMutI CAGTG 4 cut(s) 210, 216, 1686, 1905
Cac8I GCNNGC 6 cut(s) 858, 1029, 1203, 2026, 2163, 2264
CaiI CAGNNNCTG 1 cut(s) 601
CciI TCATGA 1 cut(s) 2115
CciNI GCGGCCGC 1 cut(s) 1308
CfoI GCGC 6 cut(s) 192, 478, 592, 766, 817, 2389
Cfr13I GGNCC 5 cut(s) 1082, 1215, 1700, 2059, 2211
CsiI ACCWGGT 1 cut(s) 399
Csp6I GTAC 8 cut(s) 7, 340, 397, 447, 456, 1269, 1466, 2080
CviQI GTAC 8 cut(s) 7, 340, 397, 447, 456, 1269, 1466, 2080
DdeI CTNAG 3 cut(s) 1491, 1607, 1686
DpnI GATC 5 cut(s) 36, 72, 443, 1572, 1794
DpnII GATC 5 cut(s) 34, 70, 441, 1570, 1792
DraIII CACNNNGTG 1 cut(s) 364
DriI GACNNNNNGTC 1 cut(s) 736
EaeI YGGCCR 5 cut(s) 65, 1308, 1539, 1902, 2016
EagI CGGCCG 2 cut(s) 65, 1308
Eam1104I CTCTTC 5 cut(s) 144, 426, 641, 1426, 2141
Eam1105I GACNNNNNGTC 1 cut(s) 736
EarI CTCTTC 5 cut(s) 144, 426, 641, 1426, 2141
EciI GGCGGA 1 cut(s) 713
EclXI CGGCCG 2 cut(s) 65, 1308
Eco130I CCWWGG 4 cut(s) 132, 1707, 2347, 2382
Eco47I GGWCC 3 cut(s) 1215, 2059, 2211
Eco47III AGCGCT 1 cut(s) 816
Eco52I CGGCCG 2 cut(s) 65, 1308
Eco57I CTGAAG 6 cut(s) 176, 622, 833, 1160, 1859, 2252
Eco81I CCTNAGG 1 cut(s) 1491
Eco88I CYCGRG 4 cut(s) 495, 534, 570, 2075
EcoNI CCTNNNNNAGG 1 cut(s) 936
EcoO109I RGGNCCY 1 cut(s) 1700
EcoRI GAATTC 1 cut(s) 1349
EcoRII CCWGG 4 cut(s) 399, 1647, 1936, 2318
EcoT14I CCWWGG 4 cut(s) 132, 1707, 2347, 2382
ErhI CCWWGG 4 cut(s) 132, 1707, 2347, 2382
FaqI GGGAC 4 cut(s) 227, 1076, 1184, 2234
FauI CCCGC 2 cut(s) 480, 519
FokI GGATG 7 cut(s) 162, 980, 984, 994, 1001, 1136, 2327
FspBI CTAG 5 cut(s) 450, 812, 857, 1194, 1898
FspI TGCGCA 1 cut(s) 765
GlaI GCGC 6 cut(s) 191, 477, 591, 765, 816, 2388
GsaI CCCAGC 1 cut(s) 1824
GsuI CTGGAG 1 cut(s) 623
HaeII RGCGCY 1 cut(s) 818
HaeIII GGCC 8 cut(s) 67, 1083, 1310, 1541, 1701, 1904, 2018, 2262
HapII CCGG 4 cut(s) 68, 179, 375, 1266
HhaI GCGC 6 cut(s) 192, 478, 592, 766, 817, 2389
Hin6I GCGC 6 cut(s) 190, 476, 590, 764, 815, 2387
HinP1I GCGC 6 cut(s) 190, 476, 590, 764, 815, 2387
HincII GTYRAC 2 cut(s) 1188, 1510
HindII GTYRAC 2 cut(s) 1188, 1510
HindIII AAGCTT 2 cut(s) 674, 1025
HinfI GANTC 8 cut(s) 116, 172, 575, 607, 1064, 1189, 2124, 2191
HpaI GTTAAC 1 cut(s) 1510
HpaII CCGG 4 cut(s) 68, 179, 375, 1266
HphI GGTGA 4 cut(s) 376, 415, 1292, 1304
Hpy166II GTNNAC 3 cut(s) 208, 1188, 1510
Hpy8I GTNNAC 3 cut(s) 208, 1188, 1510
HpyAV CCTTC 7 cut(s) 151, 559, 857, 938, 958, 1036, 1280
HpyCH4III ACNGT 8 cut(s) 25, 205, 220, 757, 1514, 1553, 1888, 1909
HpyCH4IV ACGT 2 cut(s) 1486, 2010
HpyCH4V TGCA 9 cut(s) 556, 595, 1031, 1534, 1645, 1655, 1717, 1777, 1919
HpyF3I CTNAG 3 cut(s) 1491, 1607, 1686
HpySE526I ACGT 2 cut(s) 1486, 2010
HspAI GCGC 6 cut(s) 190, 476, 590, 764, 815, 2387
Kpn2I TCCGGA 1 cut(s) 178
KspAI GTTAAC 1 cut(s) 1510
Kzo9I GATC 5 cut(s) 34, 70, 441, 1570, 1792
LguI GCTCTTC 2 cut(s) 144, 641
LmnI GCTCC 2 cut(s) 822, 2348
LweI GCATC 4 cut(s) 900, 1543, 2205, 2305
MabI ACCWGGT 1 cut(s) 399
MaeI CTAG 5 cut(s) 450, 812, 857, 1194, 1898
MaeII ACGT 2 cut(s) 1486, 2010
MaeIII GTNAC 5 cut(s) 656, 949, 1514, 1755, 1943
MalI GATC 5 cut(s) 36, 72, 443, 1572, 1794
MbiI CCGCTC 3 cut(s) 634, 981, 2343
MboI GATC 5 cut(s) 34, 70, 441, 1570, 1792
MfeI CAATTG 1 cut(s) 1154
MflI RGATCY 1 cut(s) 34
MlsI TGGCCA 3 cut(s) 1541, 1904, 2018
MluNI TGGCCA 3 cut(s) 1541, 1904, 2018
MlyI GAGTC 3 cut(s) 601, 1058, 1183
MmeI TCCRAC 1 cut(s) 12
Mox20I TGGCCA 3 cut(s) 1541, 1904, 2018
MroI TCCGGA 1 cut(s) 178
MscI TGGCCA 3 cut(s) 1541, 1904, 2018
MseI TTAA 5 cut(s) 92, 1509, 1545, 2094, 2369
MslI CAYNNNNRTG 1 cut(s) 726
Msp20I TGGCCA 3 cut(s) 1541, 1904, 2018
MspA1I CMGCKG 3 cut(s) 484, 598, 647
MspI CCGG 4 cut(s) 68, 179, 375, 1266
MspR9I CCNGG 4 cut(s) 401, 1649, 1938, 2320
MunI CAATTG 1 cut(s) 1154
Mva1269I GAATGC 2 cut(s) 2045, 2100
MvaI CCWGG 4 cut(s) 401, 1649, 1938, 2320
MvnI CGCG 2 cut(s) 192, 1307
NcoI CCATGG 2 cut(s) 132, 1707
NdeII GATC 5 cut(s) 34, 70, 441, 1570, 1792
NheI GCTAGC 1 cut(s) 856
NlaIV GGNNCC 1 cut(s) 330
NmeAIII GCCGAG 2 cut(s) 43, 1525
NmuCI GTSAC 2 cut(s) 656, 949
NotI GCGGCCGC 1 cut(s) 1308
NsbI TGCGCA 1 cut(s) 765
NspI RCATGY 1 cut(s) 1508
PaeR7I CTCGAG 1 cut(s) 2075
PagI TCATGA 1 cut(s) 2115
PciSI GCTCTTC 2 cut(s) 144, 641
PctI GAATGC 2 cut(s) 2045, 2100
PfeI GAWTC 5 cut(s) 116, 172, 575, 2124, 2191
PflFI GACNNNGTC 2 cut(s) 668, 2215
PleI GAGTC 3 cut(s) 601, 1058, 1183
PpsI GAGTC 3 cut(s) 601, 1058, 1183
PshBI ATTAAT 1 cut(s) 2369
Psp6I CCWGG 4 cut(s) 399, 1647, 1936, 2318
PspFI CCCAGC 1 cut(s) 1820
PspGI CCWGG 4 cut(s) 399, 1647, 1936, 2318
PspN4I GGNNCC 1 cut(s) 330
PspPI GGNCC 5 cut(s) 1082, 1215, 1700, 2059, 2211
PsrI GAACNNNNNNTAC 2 cut(s) 1449, 1481
PstI CTGCAG 3 cut(s) 558, 597, 1657
PstNI CAGNNNCTG 1 cut(s) 601
PsuI RGATCY 1 cut(s) 34
PsyI GACNNNGTC 2 cut(s) 668, 2215
PvuII CAGCTG 3 cut(s) 484, 598, 647
RsaI GTAC 8 cut(s) 8, 341, 398, 448, 457, 1270, 1467, 2081
RsaNI GTAC 8 cut(s) 7, 340, 397, 447, 456, 1269, 1466, 2080
RseI CAYNNNNRTG 1 cut(s) 726
SapI GCTCTTC 2 cut(s) 144, 641
SaqAI TTAA 5 cut(s) 92, 1509, 1545, 2094, 2369
Sau3AI GATC 5 cut(s) 34, 70, 441, 1570, 1792
Sau96I GGNCC 5 cut(s) 1082, 1215, 1700, 2059, 2211
ScaI AGTACT 3 cut(s) 8, 448, 457
SchI GAGTC 3 cut(s) 601, 1058, 1183
ScrFI CCNGG 4 cut(s) 401, 1649, 1938, 2320
SexAI ACCWGGT 1 cut(s) 399
SfaNI GCATC 4 cut(s) 900, 1543, 2205, 2305
SfcI CTRYAG 5 cut(s) 554, 593, 692, 1653, 1674
Sfr274I CTCGAG 1 cut(s) 2075
SinI GGWCC 3 cut(s) 1215, 2059, 2211
SlaI CTCGAG 1 cut(s) 2075
SmiMI CAYNNNNRTG 1 cut(s) 726
SmlI CTYRAG 3 cut(s) 821, 1478, 2075
SmoI CTYRAG 3 cut(s) 821, 1478, 2075
SpeI ACTAGT 1 cut(s) 449
SspMI CTAG 5 cut(s) 450, 812, 857, 1194, 1898
StyD4I CCNGG 4 cut(s) 399, 1647, 1936, 2318
StyI CCWWGG 4 cut(s) 132, 1707, 2347, 2382
TaaI ACNGT 8 cut(s) 25, 205, 220, 757, 1514, 1553, 1888, 1909
TaiI ACGT 2 cut(s) 1489, 2013
TaqI TCGA 8 cut(s) 168, 273, 1353, 1388, 1578, 1971, 2076, 2199
TaqII GACCGA 4 cut(s) 18, 644, 1863, 2047
TatI WGTACW 3 cut(s) 6, 446, 455
TauI GCSGC 7 cut(s) 236, 392, 481, 984, 1310, 1313, 1925
TfiI GAWTC 5 cut(s) 116, 172, 575, 2124, 2191
Tru1I TTAA 5 cut(s) 92, 1509, 1545, 2094, 2369
Tru9I TTAA 5 cut(s) 92, 1509, 1545, 2094, 2369
TscAI CASTG 4 cut(s) 210, 223, 1686, 1912
TseFI GTSAC 2 cut(s) 656, 949
Tsp45I GTSAC 2 cut(s) 656, 949
TspDTI ATGAA 8 cut(s) 56, 329, 737, 1044, 1055, 1299, 1377, 2116
TspGWI ACGGA 4 cut(s) 357, 677, 730, 1000
TspRI CASTG 4 cut(s) 210, 223, 1686, 1912
Tth111I GACNNNGTC 2 cut(s) 668, 2215
VpaK11BI GGWCC 3 cut(s) 1215, 2059, 2211
VspI ATTAAT 1 cut(s) 2369
XagI CCTNNNNNAGG 1 cut(s) 936
XapI RAATTY 4 cut(s) 886, 1133, 1349, 2086
XceI RCATGY 1 cut(s) 1508
XhoI CTCGAG 1 cut(s) 2075
XspI CTAG 5 cut(s) 450, 812, 857, 1194, 1898
ZrmI AGTACT 3 cut(s) 8, 448, 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.