Rh5BG018700

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000724
Physical Location & Seq
Forward (+)
1409019 .. 1419038
10020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG018700.1

Sequence Viewer

Length: 2181 bp
ATGGCAGAAAATAGCAGCATAAATCCCAAACACCTATATCAAAGCCTAAAGAATGGTGACAAAGCCAAAGTGATGGAATATTGTTATGATAAAACTAGATCTGATGATGAAAGGTTGAAGCTCATTCCAACAGTGCACGATGATACAATCCTCCATTTAGCAATTAACATGGGGCAGCAAGACATAGCAAAAGAAATCGTCAAAAGATGTTCCGGGGGTTCTAATGATAAGCTACTTATGAAGAAGAATGCCCTCGGAAACACAGTTCTGCACGAGGCTGCAGCAACAAGCATGACAAGCCTTGTGGAGGAGTTGTTAAGAAAGGCATCAGACTTGTTGTCTATTTCGAACAACAAAAATGAAATGCCTCTCTATACGGCAGCTTACTATGGCCAAACCGAAATGTTCAAATTGTTGGCGGACGAAGTAGACAAAAACCAGTTTGGAAATCTTGATCTTGATCTTCATTTGCGACCTAGGGGTCGTAAGACGAACCCCAATAAAGTGCGGACTACCATTCTTCATGCTGCAATACATGCCGAGTTCTTTGAGCTTGCACTTCTGATAGCAAAAAGGTATACGCAACTAGTTAAGAAAAAGGACGAAAATCGGCTGACAGGCCTTCAGCTGCTTTCATGTAATCCTTCAGCTTTCAGGAGTGGAACCAAATATGGACCGATCAAGAGGTTCATTTATTCCTGTGCTCCACATGAGAATGTGATAATGGATAAAAATGGCAGCCACGAACACGAAGAGGCTGATGAATACATTTTCAGCGATCTAGGTGACGAGGAAAATACAGCTTGCTTCAAGATGCGTCAGAGTCAAACATGTACATATATGAATAACTGCTGCACCATTGCTTTACGGCAAGCCATCTCTTCTGTTAATAGAGCGGTTTGGAAAATTCTTCAAGCTTGGGGCACAATGAAGAGGATCTATGGGGACAAGAAGAGGCATGAATCAGCCTTCAAACTAGCCAAGTTACTAATTGCAGCAGACTTTTCATGGGACAGCATAGGAGTACAAGATGAAATCGCTAGCACAAAGGTCACCGCTGCTACAAATGATGCTCCCTCTTTGGATCGCAAAGCTGATATTGAATGGATAGAAGCCCTTTTAGGTGCAGAAGCCTTAAGTGGCAGCGGTGCTGTTAGTGGCGGCCCAGAAATAGCAGCTTCATCCATGTCAGAACTTCATTCACCTCCAACGCCATTGCTCCTTGCAACAAGCCACGGAATACTAGAAATTGTGGATGAGATACTTAAAGTCTACCCTCTCGCAGTTGAGCATATAAGTGAAAAGGGGCAAAACTTGCTGCATGTTGCTGTTCTGCACCGCCATATGGACGTCTTTCAACGAGTCATTAGGATGGAATCACCCAAGTCAAGGTTGGTTCGACGTATCGACAACAATGGATTTACAATCTTGCACCATGTTGGAAACATGGCCTTTTACAAAGGAAGCACACAGCCTGGTCCTGCACTCCAGTTGCAAGAAGAGTTAGAATGGTTTGAGATTGTGGAGCAGGTGATTCCACCCCATTATGCGATGCAACAAGCCTGGAGCGAAAAGGAACAGAAAAACGAGACCCCCAAACAGTACTTTCGTCGCACACATGGCGAGCTTCTCAAGGAGGCCCAAGATTGGATAAAGCGGACCGCCGAGTCGTGCTCAACGGTGGCAGGACTAATGGCCACCGTCGCTTTCGCCGCCGCCTTCACCGTCCCTGGAGGCAACGACGAGAAAAGCGGCACTCCACTCCTTCTTCACAGCCCTTTCTTCTTAGTCTTCATCATCACCAACGCTCTCTCTCTAGCCAGCTCGCTGACGTCACTCGTCATGTTCCTCTCGATCCTGACGTCACTGTACGAGATCGACGACTTCTGCTACTCTCTCCCTCGGATGCTTATCCTCGGCTTCACCTTCCTCTTCTTCTCGGTGGCGGTGACCATGCTGGCCTTTGCTTCCACACTGATGCTGACAATTTCGACAAAGAAGAGCCTGACAACAACTTTTATATACTGCGTTGCGTTTCTTCCGGTCACTATGTTCGCGCTGTTGCAGTTCCCTTTGTACGTGGCCTTCAAAACGACTCTCCTGTACTCTGTACAGGCCATCCAAAGCGTTCTTCCGTGGCATCTCATCTGTGTTGGTTTTTCAAAACTCAAAAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

726

Amino Acids

81.29

Weight (kDa)

7.09

Isoelectric Point (pI)

38.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 51 - 147 1.8e-09 Ankyrin repeats (3 copies)
PGG PF13962 549 - 661 2.1e-24 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1519
AasI GACNNNNNNGTC 2 cut(s) 480, 1666
AatII GACGTC 3 cut(s) 1353, 1835, 1865
Acc36I ACCTGC 1 cut(s) 1519
AccBSI CCGCTC 1 cut(s) 896
AccI GTMKAC 3 cut(s) 429, 578, 1272
AccII CGCG 1 cut(s) 2057
AclWI GGATC 3 cut(s) 944, 1092, 1849
AcoI YGGCCR 2 cut(s) 391, 1695
AcsI RAATTY 1 cut(s) 906
AcuI CTGAAG 2 cut(s) 608, 630
AcyI GRCGYC 3 cut(s) 1350, 1832, 1862
AfaI GTAC 7 cut(s) 835, 1026, 1604, 1871, 2078, 2105, 2112
AfiI CCNNNNNNNGG 4 cut(s) 307, 1345, 1389, 1647
AflII CTTAAG 1 cut(s) 1135
AflIII ACRYGT 1 cut(s) 830
AgsI TTSAA 9 cut(s) 118, 409, 811, 914, 973, 1103, 1358, 2089, 2163
AhdI GACNNNNNGTC 1 cut(s) 337
AhlI ACTAGT 1 cut(s) 586
AjnI CCWGG 3 cut(s) 1474, 1562, 1729
AjuI GAANNNNNNNTTGG 2 cut(s) 2115, 2147
AloI GAACNNNNNNTCC 2 cut(s) 249, 281
Alw21I GWGCWC 3 cut(s) 138, 706, 1676
Alw26I GTCTC 1 cut(s) 1583
Alw44I GTGCAC 1 cut(s) 134
AlwI GGATC 3 cut(s) 944, 1092, 1849
AoxI GGCC 9 cut(s) 391, 619, 1162, 1449, 1638, 1695, 1959, 2082, 2115
ApaLI GTGCAC 1 cut(s) 134
ApoI RAATTY 1 cut(s) 906
AspA2I CCTAGG 1 cut(s) 476
AspLEI GCGC 1 cut(s) 2059
AspS9I GGNCC 5 cut(s) 674, 1163, 1478, 1639, 1659
AsuC2I CCSGG 1 cut(s) 214
AsuII TTCGAA 1 cut(s) 347
AsuNHI GCTAGC 1 cut(s) 1040
AvaII GGWCC 3 cut(s) 674, 1478, 1659
AvrII CCTAGG 1 cut(s) 476
BaeGI GKGCMC 2 cut(s) 138, 926
BalI TGGCCA 2 cut(s) 393, 1697
BarI GAAGNNNNNNTAC 2 cut(s) 2069, 2101
BauI CACGAG 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 1783
Bbv12I GWGCWC 3 cut(s) 138, 706, 1676
BccI CCATC 4 cut(s) 67, 884, 1366, 2126
BceAI ACGGC 2 cut(s) 393, 884
BciT130I CCWGG 3 cut(s) 1476, 1564, 1731
BcnI CCSGG 1 cut(s) 214
BcoDI GTCTC 1 cut(s) 1583
BcuI ACTAGT 1 cut(s) 586
BfaI CTAG 8 cut(s) 96, 477, 587, 782, 977, 1041, 1244, 1817
BfmI CTRYAG 1 cut(s) 279
BfrI CTTAAG 1 cut(s) 1135
BfuAI ACCTGC 1 cut(s) 1519
BglII AGATCT 1 cut(s) 98
BlnI CCTAGG 1 cut(s) 476
BmcAI AGTACT 1 cut(s) 1604
Bme1390I CCNGG 4 cut(s) 214, 1476, 1564, 1731
Bme18I GGWCC 3 cut(s) 674, 1478, 1659
BmeRI GACNNNNNGTC 1 cut(s) 337
BmgT120I GGNCC 5 cut(s) 674, 1163, 1478, 1639, 1659
BmiI GGNNCC 1 cut(s) 664
BmrFI CCNGG 4 cut(s) 214, 1476, 1564, 1731
BmsI GCATC 7 cut(s) 335, 804, 1060, 1542, 1896, 1968, 2149
BmtI GCTAGC 1 cut(s) 1044
BpiI GAAGAC 1 cut(s) 1783
BplI GAGNNNNNCTC 2 cut(s) 1658, 1690
BpmI CTGGAG 3 cut(s) 1472, 1585, 1752
Bpu14I TTCGAA 1 cut(s) 347
BpuEI CTTGAG 1 cut(s) 1616
BpuMI CCSGG 1 cut(s) 214
BsaAI YACGTR 1 cut(s) 2080
BsaBI GATNNNNATC 2 cut(s) 459, 1910
BsaHI GRCGYC 3 cut(s) 1350, 1832, 1862
BsaI GGTCTC 1 cut(s) 1583
BsaJI CCNNGG 8 cut(s) 213, 253, 476, 1234, 1729, 1901, 1915, 2135
BsaWI WCCGGW 1 cut(s) 2041
BsaXI ACNNNNNCTCC 2 cut(s) 299, 329
Bsc4I CCNNNNNNNGG 4 cut(s) 307, 1345, 1389, 1647
Bse1I ACTGG 2 cut(s) 439, 1489
Bse3DI GCAATG 2 cut(s) 858, 1214
Bse8I GATNNNNATC 2 cut(s) 459, 1910
BseBI CCWGG 3 cut(s) 1476, 1564, 1731
BseDI CCNNGG 8 cut(s) 213, 253, 476, 1234, 1729, 1901, 1915, 2135
BseGI GGATG 5 cut(s) 1181, 1261, 1377, 1911, 2118
BseJI GATNNNNATC 2 cut(s) 459, 1910
BseLI CCNNNNNNNGG 4 cut(s) 307, 1345, 1389, 1647
BseMI GCAATG 2 cut(s) 858, 1214
BseNI ACTGG 2 cut(s) 439, 1489
BseRI GAGGAG 1 cut(s) 323
BseSI GKGCMC 2 cut(s) 138, 926
BsgI GTGCAG 5 cut(s) 254, 838, 1146, 1319, 1467
Bsh1236I CGCG 1 cut(s) 2057
BshFI GGCC 9 cut(s) 393, 621, 1164, 1451, 1640, 1697, 1961, 2084, 2117
BsiHKAI GWGCWC 3 cut(s) 138, 706, 1676
BsiSI CCGG 2 cut(s) 213, 2042
BslFI GGGAC 3 cut(s) 959, 1025, 1712
BslI CCNNNNNNNGG 4 cut(s) 307, 1345, 1389, 1647
BsmAI GTCTC 1 cut(s) 1583
BsmFI GGGAC 3 cut(s) 959, 1025, 1712
BsmI GAATGC 1 cut(s) 253
BsnI GGCC 9 cut(s) 393, 621, 1164, 1451, 1640, 1697, 1961, 2084, 2117
Bso31I GGTCTC 1 cut(s) 1583
Bsp119I TTCGAA 1 cut(s) 347
Bsp1286I GDGCHC 4 cut(s) 138, 706, 926, 1676
Bsp1407I TGTACA 2 cut(s) 833, 2110
Bsp143I GATC 9 cut(s) 98, 454, 460, 678, 778, 936, 1084, 1854, 1875
BspANI GGCC 9 cut(s) 393, 621, 1164, 1451, 1640, 1697, 1961, 2084, 2117
BspFNI CGCG 1 cut(s) 2057
BspLI GGNNCC 1 cut(s) 664
BspMAI CTGCAG 1 cut(s) 283
BspMI ACCTGC 1 cut(s) 1519
BspOI GCTAGC 1 cut(s) 1044
BspPI GGATC 3 cut(s) 944, 1092, 1849
BspQI GCTCTTC 1 cut(s) 1994
BspT104I TTCGAA 1 cut(s) 347
BspTI CTTAAG 1 cut(s) 1135
BspTNI GGTCTC 1 cut(s) 1583
BsrBI CCGCTC 1 cut(s) 896
BsrDI GCAATG 2 cut(s) 858, 1214
BsrGI TGTACA 2 cut(s) 833, 2110
BsrI ACTGG 2 cut(s) 439, 1489
BssECI CCNNGG 8 cut(s) 213, 253, 476, 1234, 1729, 1901, 1915, 2135
BssMI GATC 9 cut(s) 98, 454, 460, 678, 778, 936, 1084, 1854, 1875
BssNAI GTATAC 1 cut(s) 579
BssNI GRCGYC 3 cut(s) 1350, 1832, 1862
BssSI CACGAG 1 cut(s) 272
BssT1I CCWWGG 1 cut(s) 476
Bst1107I GTATAC 1 cut(s) 579
Bst2BI CACGAG 1 cut(s) 272
Bst2UI CCWGG 3 cut(s) 1476, 1564, 1731
Bst4CI ACNGT 7 cut(s) 133, 265, 1602, 1681, 1702, 1726, 1869
Bst6I CTCTTC 7 cut(s) 747, 886, 926, 947, 1494, 1937, 1994
BstACI GRCGYC 3 cut(s) 1350, 1832, 1862
BstAFI CTTAAG 1 cut(s) 1135
BstAPI GCANNNNNTGC 2 cut(s) 536, 1315
BstAUI TGTACA 2 cut(s) 833, 2110
BstBAI YACGTR 1 cut(s) 2080
BstBI TTCGAA 1 cut(s) 347
BstC8I GCNNGC 8 cut(s) 555, 805, 873, 1042, 1625, 1822, 1826, 1959
BstDEI CTNAG 1 cut(s) 1786
BstDSI CCRYGG 2 cut(s) 1234, 2135
BstEII GGTNACC 2 cut(s) 1051, 1948
BstENI CCTNNNNNAGG 1 cut(s) 305
BstF5I GGATG 5 cut(s) 1181, 1261, 1377, 1911, 2118
BstFNI CGCG 1 cut(s) 2057
BstHHI GCGC 1 cut(s) 2059
BstKTI GATC 9 cut(s) 101, 457, 463, 681, 781, 939, 1087, 1857, 1878
BstMAI GTCTC 1 cut(s) 1583
BstMBI GATC 9 cut(s) 98, 454, 460, 678, 778, 936, 1084, 1854, 1875
BstMWI GCNNNNNNNGC 6 cut(s) 297, 536, 1315, 1620, 1703, 1712
BstNI CCWGG 3 cut(s) 1476, 1564, 1731
BstNSI RCATGY 3 cut(s) 539, 834, 1325
BstPI GGTNACC 2 cut(s) 1051, 1948
BstSCI CCNGG 4 cut(s) 212, 1474, 1562, 1729
BstSFI CTRYAG 1 cut(s) 279
BstSLI GKGCMC 2 cut(s) 138, 926
BstUI CGCG 1 cut(s) 2057
BstV2I GAAGAC 1 cut(s) 1783
BstX2I RGATCY 2 cut(s) 98, 936
BstXI CCANNNNNNTGG 1 cut(s) 73
BstYI RGATCY 2 cut(s) 98, 936
BstZ17I GTATAC 1 cut(s) 579
BsuRI GGCC 9 cut(s) 393, 621, 1164, 1451, 1640, 1697, 1961, 2084, 2117
BtgI CCRYGG 2 cut(s) 1234, 2135
BtgZI GCGATG 1 cut(s) 1565
BtsCI GGATG 5 cut(s) 1181, 1261, 1377, 1911, 2118
BtsIMutI CAGTG 3 cut(s) 138, 1865, 1973
BveI ACCTGC 1 cut(s) 1519
Cac8I GCNNGC 8 cut(s) 555, 805, 873, 1042, 1625, 1822, 1826, 1959
CfoI GCGC 1 cut(s) 2059
Cfr13I GGNCC 5 cut(s) 674, 1163, 1478, 1639, 1659
CpoI CGGWCCG 1 cut(s) 1659
CseI GACGC 1 cut(s) 806
Csp6I GTAC 7 cut(s) 834, 1025, 1603, 1870, 2077, 2104, 2111
CspCI CAANNNNNGTGG 2 cut(s) 285, 320
CspI CGGWCCG 1 cut(s) 1659
CviQI GTAC 7 cut(s) 834, 1025, 1603, 1870, 2077, 2104, 2111
DdeI CTNAG 1 cut(s) 1786
DpnI GATC 9 cut(s) 100, 456, 462, 680, 780, 938, 1086, 1856, 1877
DpnII GATC 9 cut(s) 98, 454, 460, 678, 778, 936, 1084, 1854, 1875
DrdI GACNNNNNNGTC 2 cut(s) 480, 1666
DriI GACNNNNNGTC 1 cut(s) 337
DseDI GACNNNNNNGTC 2 cut(s) 480, 1666
EaeI YGGCCR 2 cut(s) 391, 1695
Eam1104I CTCTTC 7 cut(s) 747, 886, 926, 947, 1494, 1937, 1994
Eam1105I GACNNNNNGTC 1 cut(s) 337
EarI CTCTTC 7 cut(s) 747, 886, 926, 947, 1494, 1937, 1994
EciI GGCGGA 1 cut(s) 434
Eco130I CCWWGG 1 cut(s) 476
Eco147I AGGCCT 1 cut(s) 621
Eco31I GGTCTC 1 cut(s) 1583
Eco47I GGWCC 3 cut(s) 674, 1478, 1659
Eco57I CTGAAG 2 cut(s) 608, 630
Eco91I GGTNACC 2 cut(s) 1051, 1948
EcoNI CCTNNNNNAGG 1 cut(s) 305
EcoO65I GGTNACC 2 cut(s) 1051, 1948
EcoRII CCWGG 3 cut(s) 1474, 1562, 1729
EcoT14I CCWWGG 1 cut(s) 476
ErhI CCWWGG 1 cut(s) 476
FaqI GGGAC 3 cut(s) 959, 1025, 1712
FauNDI CATATG 1 cut(s) 1344
FblI GTMKAC 3 cut(s) 429, 578, 1272
FokI GGATG 5 cut(s) 1168, 1268, 1384, 1918, 2105
FspBI CTAG 8 cut(s) 96, 477, 587, 782, 977, 1041, 1244, 1817
GlaI GCGC 1 cut(s) 2058
GsuI CTGGAG 3 cut(s) 1472, 1585, 1752
HaeIII GGCC 9 cut(s) 393, 621, 1164, 1451, 1640, 1697, 1961, 2084, 2117
HapII CCGG 2 cut(s) 213, 2042
HgaI GACGC 1 cut(s) 806
HhaI GCGC 1 cut(s) 2059
Hin1I GRCGYC 3 cut(s) 1350, 1832, 1862
Hin6I GCGC 1 cut(s) 2057
HinP1I GCGC 1 cut(s) 2057
HindIII AAGCTT 1 cut(s) 915
HinfI GANTC 7 cut(s) 823, 962, 1362, 1376, 1534, 1667, 2095
HpaII CCGG 2 cut(s) 213, 2042
Hpy166II GTNNAC 4 cut(s) 136, 430, 579, 1273
Hpy188I TCNGA 7 cut(s) 103, 257, 331, 564, 822, 1192, 1905
Hpy188III TCNNGA 7 cut(s) 452, 458, 655, 682, 811, 1852, 1858
Hpy8I GTNNAC 4 cut(s) 136, 430, 579, 1273
Hpy99I CGWCG 5 cut(s) 1404, 1614, 1706, 1745, 1883
HpyAV CCTTC 7 cut(s) 632, 654, 979, 1729, 1775, 1936, 2095
HpyCH4III ACNGT 7 cut(s) 133, 265, 1602, 1681, 1702, 1726, 1869
HpyCH4IV ACGT 5 cut(s) 1350, 1402, 1832, 1862, 2079
HpyF10VI GCNNNNNNNGC 6 cut(s) 297, 536, 1315, 1620, 1703, 1712
HpyF3I CTNAG 1 cut(s) 1786
HpySE526I ACGT 5 cut(s) 1350, 1402, 1832, 1862, 2079
Hsp92I GRCGYC 3 cut(s) 1350, 1832, 1862
HspAI GCGC 1 cut(s) 2057
Kzo9I GATC 9 cut(s) 98, 454, 460, 678, 778, 936, 1084, 1854, 1875
LguI GCTCTTC 1 cut(s) 1994
LmnI GCTCC 5 cut(s) 709, 1078, 1224, 1525, 1566
LweI GCATC 7 cut(s) 335, 804, 1060, 1542, 1896, 1968, 2149
MaeI CTAG 8 cut(s) 96, 477, 587, 782, 977, 1041, 1244, 1817
MaeII ACGT 5 cut(s) 1350, 1402, 1832, 1862, 2079
MaeIII GTNAC 8 cut(s) 56, 785, 984, 1051, 1833, 1863, 1948, 2044
MalI GATC 9 cut(s) 100, 456, 462, 680, 780, 938, 1086, 1856, 1877
MbiI CCGCTC 1 cut(s) 896
MboI GATC 9 cut(s) 98, 454, 460, 678, 778, 936, 1084, 1854, 1875
MflI RGATCY 2 cut(s) 98, 936
MhlI GDGCHC 4 cut(s) 138, 706, 926, 1676
MlsI TGGCCA 2 cut(s) 393, 1697
MluCI AATT 7 cut(s) 162, 410, 906, 990, 1248, 1986, 2176
MluNI TGGCCA 2 cut(s) 393, 1697
MlyI GAGTC 4 cut(s) 832, 1371, 1676, 2089
MmeI TCCRAC 3 cut(s) 152, 1232, 1420
Mox20I TGGCCA 2 cut(s) 393, 1697
MscI TGGCCA 2 cut(s) 393, 1697
MseI TTAA 7 cut(s) 165, 317, 591, 888, 1136, 1266, 2179
MslI CAYNNNNRTG 4 cut(s) 714, 1296, 1370, 1976
Msp20I TGGCCA 2 cut(s) 393, 1697
MspA1I CMGCKG 3 cut(s) 628, 1058, 1146
MspCI CTTAAG 1 cut(s) 1135
MspI CCGG 2 cut(s) 213, 2042
MspR9I CCNGG 4 cut(s) 214, 1476, 1564, 1731
Mva1269I GAATGC 1 cut(s) 253
MvaI CCWGG 3 cut(s) 1476, 1564, 1731
MvnI CGCG 1 cut(s) 2057
MwoI GCNNNNNNNGC 6 cut(s) 297, 536, 1315, 1620, 1703, 1712
NciI CCSGG 1 cut(s) 214
NdeI CATATG 1 cut(s) 1344
NdeII GATC 9 cut(s) 98, 454, 460, 678, 778, 936, 1084, 1854, 1875
NheI GCTAGC 1 cut(s) 1040
NlaIV GGNNCC 1 cut(s) 664
NmeAIII GCCGAG 3 cut(s) 565, 1690, 1896
NmuCI GTSAC 7 cut(s) 56, 785, 1051, 1833, 1863, 1948, 2044
NspI RCATGY 3 cut(s) 539, 834, 1325
NspV TTCGAA 1 cut(s) 347
PaqCI CACCTGC 1 cut(s) 1519
PceI AGGCCT 1 cut(s) 621
PciI ACATGT 1 cut(s) 830
PciSI GCTCTTC 1 cut(s) 1994
PcsI WCGNNNNNNNCGW 2 cut(s) 1859, 1878
PctI GAATGC 1 cut(s) 253
PfeI GAWTC 3 cut(s) 962, 1376, 1534
PleI GAGTC 4 cut(s) 831, 1370, 1675, 2089
PpsI GAGTC 4 cut(s) 831, 1370, 1675, 2089
Ppu21I YACGTR 1 cut(s) 2080
PscI ACATGT 1 cut(s) 830
Psp6I CCWGG 3 cut(s) 1474, 1562, 1729
PspEI GGTNACC 2 cut(s) 1051, 1948
PspGI CCWGG 3 cut(s) 1474, 1562, 1729
PspN4I GGNNCC 1 cut(s) 664
PspPI GGNCC 5 cut(s) 674, 1163, 1478, 1639, 1659
PstI CTGCAG 1 cut(s) 283
PsuI RGATCY 2 cut(s) 98, 936
PvuII CAGCTG 1 cut(s) 628
RsaI GTAC 7 cut(s) 835, 1026, 1604, 1871, 2078, 2105, 2112
RsaNI GTAC 7 cut(s) 834, 1025, 1603, 1870, 2077, 2104, 2111
RseI CAYNNNNRTG 4 cut(s) 714, 1296, 1370, 1976
Rsr2I CGGWCCG 1 cut(s) 1659
RsrII CGGWCCG 1 cut(s) 1659
SapI GCTCTTC 1 cut(s) 1994
SaqAI TTAA 7 cut(s) 165, 317, 591, 888, 1136, 1266, 2179
Sau3AI GATC 9 cut(s) 98, 454, 460, 678, 778, 936, 1084, 1854, 1875
Sau96I GGNCC 5 cut(s) 674, 1163, 1478, 1639, 1659
ScaI AGTACT 1 cut(s) 1604
SchI GAGTC 4 cut(s) 832, 1371, 1676, 2089
ScrFI CCNGG 4 cut(s) 214, 1476, 1564, 1731
SduI GDGCHC 4 cut(s) 138, 706, 926, 1676
SfaNI GCATC 7 cut(s) 335, 804, 1060, 1542, 1896, 1968, 2149
SfcI CTRYAG 1 cut(s) 279
SfuI TTCGAA 1 cut(s) 347
SinI GGWCC 3 cut(s) 674, 1478, 1659
SmiMI CAYNNNNRTG 4 cut(s) 714, 1296, 1370, 1976
SmlI CTYRAG 2 cut(s) 1135, 1631
SmoI CTYRAG 2 cut(s) 1135, 1631
SpeI ACTAGT 1 cut(s) 586
Sse9I AATT 7 cut(s) 162, 410, 906, 990, 1248, 1986, 2176
SseBI AGGCCT 1 cut(s) 621
SspI AATATT 1 cut(s) 80
SspMI CTAG 8 cut(s) 96, 477, 587, 782, 977, 1041, 1244, 1817
StuI AGGCCT 1 cut(s) 621
StyD4I CCNGG 4 cut(s) 212, 1474, 1562, 1729
StyI CCWWGG 1 cut(s) 476
TaaI ACNGT 7 cut(s) 133, 265, 1602, 1681, 1702, 1726, 1869
TaiI ACGT 5 cut(s) 1353, 1405, 1835, 1865, 2082
TaqI TCGA 6 cut(s) 347, 1399, 1407, 1853, 1878, 1991
TaqII GACCGA 1 cut(s) 691
TasI AATT 7 cut(s) 162, 410, 906, 990, 1248, 1986, 2176
TatI WGTACW 5 cut(s) 833, 1024, 1602, 2103, 2110
TauI GCSGC 4 cut(s) 1164, 1715, 1718, 1755
TfiI GAWTC 3 cut(s) 962, 1376, 1534
Tru1I TTAA 7 cut(s) 165, 317, 591, 888, 1136, 1266, 2179
Tru9I TTAA 7 cut(s) 165, 317, 591, 888, 1136, 1266, 2179
TscAI CASTG 3 cut(s) 138, 1872, 1980
TseFI GTSAC 7 cut(s) 56, 785, 1051, 1833, 1863, 1948, 2044
Tsp45I GTSAC 7 cut(s) 56, 785, 1051, 1833, 1863, 1948, 2044
TspGWI ACGGA 2 cut(s) 1251, 2124
TspRI CASTG 3 cut(s) 138, 1872, 1980
Vha464I CTTAAG 1 cut(s) 1135
VneI GTGCAC 1 cut(s) 134
VpaK11BI GGWCC 3 cut(s) 674, 1478, 1659
XagI CCTNNNNNAGG 1 cut(s) 305
XapI RAATTY 1 cut(s) 906
XceI RCATGY 3 cut(s) 539, 834, 1325
XcmI CCANNNNNNNNNTGG 1 cut(s) 1390
XmaJI CCTAGG 1 cut(s) 476
XmiI GTMKAC 3 cut(s) 429, 578, 1272
XspI CTAG 8 cut(s) 96, 477, 587, 782, 977, 1041, 1244, 1817
ZraI GACGTC 3 cut(s) 1351, 1833, 1863
ZrmI AGTACT 1 cut(s) 1604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.