pycom05g32050

Ankyrin repeat-containing protein At3g12360-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000724
Physical Location & Seq
Reverse (-)
31590247 .. 31593317
3071 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32050.3

Sequence Viewer

Length: 2652 bp
ATGGCGTACTGGAAAACGCTAACGGTTCGGCCGGATCCGATGAAAGAGAGAGTGGATAGCTCTCGGCCGGATCAGAAAGAGAGAGAGGATAGCTCTCGGCTGGATCAAAAAGAGAGAGAGAGATTAAAGAAAATGGAAGAGTTCAAAGATTCCTATAAATATGCCATGAATGAGGAATGTGAAGGGCTGAAGGAAAAGTTCGAGAATCATCCGGAAATAATGCGCGATGAAATTACAGTGGACGGGGACACTGTATTTCACCTTGCGGCTTCTTACAGCAGCAAATCACAAGGTAAAAATGTCCTCGAAATGCTTATCAATATACTCGGTTCAACCAATGACAAGAAGAAAGCTCTGAGGGATGCAAATAAGTACGGAAACAATACTCTCCACGAGGTGAGTGTGTCCGGCAATAAGGAAGCGGCAGAGTACCTGTTGAGCAACTTCAACGAGCCTCTTCCAGAAGAGGTCATGATCAGTACTAGTAGTACTGATAAAGAGAATTGTGCGCCGCAGCTGCGGGAGGCTCGGGGTTCAGAGAATGATGAGCTTCAGCGGAAGGCTCGGGATTCAGAGAATTATGCGCTGCGGCTGCTGGAGACTCGGAACTATTTAGGAGAAACTCCGCTCTTCAGGGCGGCTGCTCTCGGTCACACAGACTTGGTCAAGCTTTATGCCGACAAACTACCGGCAGACAATCTTTGGAGGCACTTCCACAGAGATGACAAAATGTCCGTTCTTCATATCACAGTCGTTGCGCAACATTTTGGTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTATGTGAGAACCCAGACTGCTATTTGGTTATTGGAGGAACACCCATATCTAGCGCCCCTTGAGGGAGATAAAGGATTGACAAGCCTTCAGTTGCTAGCCCAAATGCCAACTGCCTTTTATCCAAATTTTGAAGAAAGCAAATGGAAGATGCTAATTTATTATTGTAAGTACCATTACCCTACAAACTGCTATATGTGTGCGCACATACGCATGGTATATATACACAGGTGCAAGCATGTTTTTTTTTTTTTTTTTTTTTTAAATGAAGCAGTAATCTCATACTTAATATCATTATATATGCAAGCCTTCCTGATGGAGGAAATGTGGTCACACCACATCAGAAGGATGATGTGGAGAGCGGCATGGATGGCAACCATCCCCGTTTATCCGTCTTTCGGAAAAAGCTTGCAGGTAATCAAATTTGTAATCTCATTAATTGATCGAATTGGATTTTTTTTTCACTATTCTAACGAAGTTGCATGCATTAAATGTTTGGCAGTTGTTATGAAGGTTTACTATTCATTATGGGAATCCCTTGCTAAAGTTAATGTATACATATGCTTGACCATGCGATGGATAGGAATAGGAGGCCCACAGGAAGAGGTAATCTACATGATATGGAAGGAGAAGAAAAACAAAAAATTTCTGAAGAAACTCATCCAATTGCTCGTCAAGTTGGACGACTATTCTTTGTTGACTCCTAGTAAGCAAGCAAAAATCAGGACCATTTCTCTAGGGGAAAAACCTGATAAAGAAAAGGAAGCAACCAAATACGGTACGACAAATGGCGATGAAGGAGGTGAAAAAGGAAACGCGGCCGCAGATAAGCATTTCTCTTGGGTCAGGGAGATAACCGAATTGGATAGATGTGAACAAGAAAAGGAAGCAACCAAATCGAGAAAGGAGGCGAGGAGTGAATTTTACAACTCTTCCCCATTGCTTATAGCAACTATCAAAGGAATTGAACCCATTGTGCGGGAGATACTTGAGCAGCGTCCTCAGACAGCCGAGCATGTTAACCGTTACGAACGCAACATTTTCGAACCAAAACTGATGTCAAGGCTGAATAAGAGGATAGACCACAATGGAAACACCATATTGCACCAGGCTGCGGATAGAACTTACTACTCTATAGCAGTGTCTCAGAAAGTAATAGGCCCTGCCATGGAACTGCAAAAAGAGCTGCTCTGGAAGCAGAGCTGTTACCACCTCATTACATCATGCACCACAACGACAATGATCAGACAGCGGAGGAGTTGTTCAATGCTGGGCATGATCGATCTTCTGAAGTCCGCACAAGAATGGGTAAAAGCAACGGCTGGGTCATGCTCAACCGTGGCGGTGCTAGTGGCCACTGTGGTCTTTGCAGCCGCCTATGCCATTCCTGGGGGTAACAACGAGCAAAGTGGTCGTCCTGTTTTCGAGAACAATCCTCTCTTTTTGCTCTTTACCAGCATGGACGTTGTGGCCATCGCCTGCTCATTATCTTCTGTGGCATTCTTTCTCTCGGTCCTCTCCTCCCCTCTCGAGTACCCATATTTCGTTAAAAGCATTCCTCACAAGGTCATGATAGGATTCGTCTTTCTCTTCGTGTCCATGGCAACCACCATGCTCGCCTTTGCTGCCACTATTTTGCTCTTGATTCGCATCGAGAAAAAATGGACCAAGTCTCTACTTTACCCTATTGCCTTTTTCCCAGTCCCTCTATTCGGCCTGCTTCAGTTTCCTATGTATCAATTTGTCATAAACATGTTTCTGAAAATGGATGCCTGGTTTTTCAAACCCTTATTCCGCTTCTTTGGCTTTCGCAAGAAGAAATTAATTTGGAGGAAGATTAATGCGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

884

Amino Acids

102.21

Weight (kDa)

8.99

Isoelectric Point (pI)

44.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 3 cut(s) 759, 1005, 2646
Acc36I ACCTGC 1 cut(s) 1204
AccB7I CCANNNNNTGG 1 cut(s) 1377
AccBSI CCGCTC 2 cut(s) 628, 1163
AccI GTMKAC 1 cut(s) 1356
AccII CGCG 2 cut(s) 225, 1619
AccIII TCCGGA 1 cut(s) 211
AclWI GGATC 4 cut(s) 29, 42, 78, 111
AcoI YGGCCR 5 cut(s) 29, 65, 1620, 2154, 2271
AcsI RAATTY 4 cut(s) 928, 1223, 1445, 1721
AcuI CTGAAG 7 cut(s) 209, 536, 616, 875, 1472, 2111, 2507
AdeI CACNNNGTG 1 cut(s) 397
AfaI GTAC 8 cut(s) 8, 374, 431, 481, 490, 974, 1582, 2336
AfiI CCNNNNNNNGG 9 cut(s) 866, 1120, 1199, 1377, 1779, 1915, 1969, 2190, 2513
AflIII ACRYGT 1 cut(s) 2553
AgsI TTSAA 7 cut(s) 145, 333, 448, 935, 1769, 2067, 2584
AhdI GACNNNNNGTC 1 cut(s) 730
AhlI ACTAGT 1 cut(s) 482
AjnI CCWGG 3 cut(s) 1908, 2188, 2573
AloI GAACNNNNNNTCC 2 cut(s) 2047, 2079
AluBI AGCT 9 cut(s) 60, 93, 353, 517, 550, 670, 1209, 1987, 2004
AluI AGCT 9 cut(s) 60, 93, 353, 517, 550, 670, 1209, 1987, 2004
Alw26I GTCTC 4 cut(s) 593, 776, 1950, 2478
AlwI GGATC 4 cut(s) 29, 42, 78, 111
Ama87I CYCGRG 3 cut(s) 528, 564, 2330
Aor13HI TCCGGA 1 cut(s) 211
AoxI GGCC 8 cut(s) 29, 65, 1393, 1620, 1960, 2154, 2271, 2515
ApoI RAATTY 4 cut(s) 928, 1223, 1445, 1721
ArsI GACNNNNNNTTYG 4 cut(s) 1844, 1876, 2200, 2232
AseI ATTAAT 3 cut(s) 1238, 2624, 2640
AspLEI GCGC 7 cut(s) 225, 511, 586, 760, 859, 1006, 2647
AspS9I GGNCC 5 cut(s) 1394, 1527, 1961, 2314, 2466
AsuHPI GGTGA 3 cut(s) 251, 409, 1616
AsuII TTCGAA 1 cut(s) 1845
AsuNHI GCTAGC 1 cut(s) 898
AvaI CYCGRG 3 cut(s) 528, 564, 2330
AvaII GGWCC 3 cut(s) 1527, 2314, 2466
BalI TGGCCA 2 cut(s) 2156, 2273
BamHI GGATCC 1 cut(s) 34
BauI CACGAG 1 cut(s) 392
BccI CCATC 5 cut(s) 1111, 1165, 1187, 1371, 2282
BceAI ACGGC 1 cut(s) 2136
BcgI CGANNNNNNTGC 6 cut(s) 1680, 1714, 1825, 1859, 2195, 2229
BciT130I CCWGG 3 cut(s) 1910, 2190, 2575
BclI TGATCA 2 cut(s) 474, 2043
BcoDI GTCTC 4 cut(s) 593, 776, 1950, 2478
BcuI ACTAGT 1 cut(s) 482
BfaI CTAG 6 cut(s) 483, 854, 899, 1506, 1538, 2150
BfmI CTRYAG 1 cut(s) 1935
BfoI RGCGCY 1 cut(s) 860
BfuAI ACCTGC 1 cut(s) 1204
BmcAI AGTACT 2 cut(s) 481, 490
Bme1390I CCNGG 3 cut(s) 1910, 2190, 2575
Bme18I GGWCC 3 cut(s) 1527, 2314, 2466
BmeRI GACNNNNNGTC 1 cut(s) 730
BmeT110I CYCGRG 3 cut(s) 528, 564, 2330
BmgT120I GGNCC 5 cut(s) 1394, 1527, 1961, 2314, 2466
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 3 cut(s) 1910, 2190, 2575
BmrI ACTGGG 1 cut(s) 2495
BmsI GCATC 4 cut(s) 352, 942, 2460, 2560
BmtI GCTAGC 1 cut(s) 902
BmuI ACTGGG 1 cut(s) 2495
BplI GAGNNNNNCTC 2 cut(s) 77, 109
BpmI CTGGAG 1 cut(s) 617
Bpu14I TTCGAA 1 cut(s) 1845
BpuEI CTTGAG 2 cut(s) 884, 1811
Bsa29I ATCGAT 1 cut(s) 2082
BsaBI GATNNNNATC 1 cut(s) 2450
BsaI GGTCTC 1 cut(s) 776
BsaJI CCNNGG 4 cut(s) 1968, 2139, 2189, 2400
BsaWI WCCGGW 1 cut(s) 211
BsaXI ACNNNNNCTCC 4 cut(s) 1112, 1142, 2047, 2077
Bsc4I CCNNNNNNNGG 9 cut(s) 866, 1120, 1199, 1377, 1779, 1915, 1969, 2190, 2513
Bse118I RCCGGY 1 cut(s) 688
Bse1I ACTGG 2 cut(s) 14, 2501
Bse3DI GCAATG 1 cut(s) 1739
Bse8I GATNNNNATC 1 cut(s) 2450
BseAI TCCGGA 1 cut(s) 211
BseBI CCWGG 3 cut(s) 1910, 2190, 2575
BseCI ATCGAT 1 cut(s) 2082
BseDI CCNNGG 4 cut(s) 1968, 2139, 2189, 2400
BseGI GGATG 7 cut(s) 208, 367, 1155, 1176, 1179, 1461, 2575
BseJI GATNNNNATC 1 cut(s) 2450
BseLI CCNNNNNNNGG 9 cut(s) 866, 1120, 1199, 1377, 1779, 1915, 1969, 2190, 2513
BseMI GCAATG 1 cut(s) 1739
BseMII CTCAG 3 cut(s) 347, 1817, 1961
BseNI ACTGG 2 cut(s) 14, 2501
BseRI GAGGAG 3 cut(s) 1729, 2071, 2311
BseX3I CGGCCG 3 cut(s) 29, 65, 1620
BseYI CCCAGC 2 cut(s) 2071, 2123
Bsh1236I CGCG 2 cut(s) 225, 1619
Bsh1285I CGRYCG 3 cut(s) 32, 68, 1623
BshFI GGCC 8 cut(s) 31, 67, 1395, 1622, 1962, 2156, 2273, 2517
BshVI ATCGAT 1 cut(s) 2082
BsiEI CGRYCG 3 cut(s) 32, 68, 1623
BsiHKCI CYCGRG 3 cut(s) 528, 564, 2330
BsiSI CCGG 5 cut(s) 32, 68, 212, 408, 689
BslFI GGGAC 2 cut(s) 260, 2489
BslI CCNNNNNNNGG 9 cut(s) 866, 1120, 1199, 1377, 1779, 1915, 1969, 2190, 2513
BsmAI GTCTC 4 cut(s) 593, 776, 1950, 2478
BsmFI GGGAC 2 cut(s) 260, 2489
BsmI GAATGC 2 cut(s) 2300, 2355
BsnI GGCC 8 cut(s) 31, 67, 1395, 1622, 1962, 2156, 2273, 2517
Bso31I GGTCTC 1 cut(s) 776
BsoBI CYCGRG 3 cut(s) 528, 564, 2330
Bsp119I TTCGAA 1 cut(s) 1845
Bsp13I TCCGGA 1 cut(s) 211
Bsp143I GATC 8 cut(s) 34, 70, 103, 474, 1243, 2043, 2079, 2083
Bsp19I CCATGG 2 cut(s) 1968, 2400
BspANI GGCC 8 cut(s) 31, 67, 1395, 1622, 1962, 2156, 2273, 2517
BspCNI CTCAG 3 cut(s) 348, 1816, 1960
BspDI ATCGAT 1 cut(s) 2082
BspEI TCCGGA 1 cut(s) 211
BspFNI CGCG 2 cut(s) 225, 1619
BspHI TCATGA 2 cut(s) 471, 2370
BspLI GGNNCC 1 cut(s) 36
BspMI ACCTGC 1 cut(s) 1204
BspOI GCTAGC 1 cut(s) 902
BspPI GGATC 4 cut(s) 29, 42, 78, 111
BspQI GCTCTTC 1 cut(s) 635
BspT104I TTCGAA 1 cut(s) 1845
BspTNI GGTCTC 1 cut(s) 776
BsrBI CCGCTC 2 cut(s) 628, 1163
BsrDI GCAATG 1 cut(s) 1739
BsrFI RCCGGY 1 cut(s) 688
BsrI ACTGG 2 cut(s) 14, 2501
BssAI RCCGGY 1 cut(s) 688
BssECI CCNNGG 4 cut(s) 1968, 2139, 2189, 2400
BssMI GATC 8 cut(s) 34, 70, 103, 474, 1243, 2043, 2079, 2083
BssNAI GTATAC 1 cut(s) 1357
BssSI CACGAG 1 cut(s) 392
BssT1I CCWWGG 2 cut(s) 1968, 2400
Bst1107I GTATAC 1 cut(s) 1357
Bst2BI CACGAG 1 cut(s) 392
Bst2UI CCWGG 3 cut(s) 1910, 2190, 2575
Bst4CI ACNGT 8 cut(s) 25, 238, 253, 751, 1580, 1826, 2140, 2161
Bst6I CTCTTC 7 cut(s) 132, 459, 462, 635, 1398, 1738, 2396
BstBI TTCGAA 1 cut(s) 1845
BstC8I GCNNGC 9 cut(s) 900, 1037, 1107, 1211, 1285, 1515, 2281, 2418, 2519
BstDEI CTNAG 3 cut(s) 356, 1803, 1947
BstDSI CCRYGG 3 cut(s) 1968, 2139, 2400
BstENI CCTNNNNNAGG 1 cut(s) 1118
BstF5I GGATG 7 cut(s) 208, 367, 1155, 1176, 1179, 1461, 2575
BstFNI CGCG 2 cut(s) 225, 1619
BstH2I RGCGCY 1 cut(s) 860
BstHHI GCGC 7 cut(s) 225, 511, 586, 760, 859, 1006, 2647
BstKTI GATC 8 cut(s) 37, 73, 106, 477, 1246, 2046, 2082, 2086
BstMAI GTCTC 4 cut(s) 593, 776, 1950, 2478
BstMBI GATC 8 cut(s) 34, 70, 103, 474, 1243, 2043, 2079, 2083
BstMCI CGRYCG 3 cut(s) 32, 68, 1623
BstMWI GCNNNNNNNGC 8 cut(s) 517, 592, 1172, 1984, 1996, 2180, 2426, 2604
BstNI CCWGG 3 cut(s) 1910, 2190, 2575
BstNSI RCATGY 4 cut(s) 1043, 1287, 1820, 2557
BstSCI CCNGG 3 cut(s) 1908, 2188, 2573
BstSFI CTRYAG 1 cut(s) 1935
BstUI CGCG 2 cut(s) 225, 1619
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
BstZ17I GTATAC 1 cut(s) 1357
BstZI CGGCCG 3 cut(s) 29, 65, 1620
Bsu15I ATCGAT 1 cut(s) 2082
BsuRI GGCC 8 cut(s) 31, 67, 1395, 1622, 1962, 2156, 2273, 2517
BsuTUI ATCGAT 1 cut(s) 2082
BtgI CCRYGG 3 cut(s) 1968, 2139, 2400
BtgZI GCGATG 4 cut(s) 240, 1390, 1608, 2260
BtsCI GGATG 7 cut(s) 208, 367, 1155, 1176, 1179, 1461, 2575
BtsI GCAGTG 1 cut(s) 1947
BtsIMutI CAGTG 4 cut(s) 243, 249, 1947, 2157
BveI ACCTGC 1 cut(s) 1204
Cac8I GCNNGC 9 cut(s) 900, 1037, 1107, 1211, 1285, 1515, 2281, 2418, 2519
CciI TCATGA 2 cut(s) 471, 2370
CciNI GCGGCCGC 1 cut(s) 1620
CfoI GCGC 7 cut(s) 225, 511, 586, 760, 859, 1006, 2647
Cfr10I RCCGGY 1 cut(s) 688
Cfr13I GGNCC 5 cut(s) 1394, 1527, 1961, 2314, 2466
ClaI ATCGAT 1 cut(s) 2082
CseI GACGC 1 cut(s) 1787
Csp6I GTAC 8 cut(s) 7, 373, 430, 480, 489, 973, 1581, 2335
CviQI GTAC 8 cut(s) 7, 373, 430, 480, 489, 973, 1581, 2335
DdeI CTNAG 3 cut(s) 356, 1803, 1947
DpnI GATC 8 cut(s) 36, 72, 105, 476, 1245, 2045, 2081, 2085
DpnII GATC 8 cut(s) 34, 70, 103, 474, 1243, 2043, 2079, 2083
DraI TTTAAA 1 cut(s) 1065
DraIII CACNNNGTG 1 cut(s) 397
DriI GACNNNNNGTC 1 cut(s) 730
EaeI YGGCCR 5 cut(s) 29, 65, 1620, 2154, 2271
EagI CGGCCG 3 cut(s) 29, 65, 1620
Eam1104I CTCTTC 7 cut(s) 132, 459, 462, 635, 1398, 1738, 2396
Eam1105I GACNNNNNGTC 1 cut(s) 730
EarI CTCTTC 7 cut(s) 132, 459, 462, 635, 1398, 1738, 2396
EclXI CGGCCG 3 cut(s) 29, 65, 1620
Eco130I CCWWGG 2 cut(s) 1968, 2400
Eco31I GGTCTC 1 cut(s) 776
Eco47I GGWCC 3 cut(s) 1527, 2314, 2466
Eco52I CGGCCG 3 cut(s) 29, 65, 1620
Eco57I CTGAAG 7 cut(s) 209, 536, 616, 875, 1472, 2111, 2507
Eco88I CYCGRG 3 cut(s) 528, 564, 2330
EcoNI CCTNNNNNAGG 1 cut(s) 1118
EcoO109I RGGNCCY 1 cut(s) 1961
EcoRII CCWGG 3 cut(s) 1908, 2188, 2573
EcoT14I CCWWGG 2 cut(s) 1968, 2400
EcoT22I ATGCAT 1 cut(s) 1289
ErhI CCWWGG 2 cut(s) 1968, 2400
FaqI GGGAC 2 cut(s) 260, 2489
FauI CCCGC 2 cut(s) 513, 1773
FauNDI CATATG 1 cut(s) 1361
FbaI TGATCA 2 cut(s) 474, 2043
FblI GTMKAC 1 cut(s) 1356
FokI GGATG 7 cut(s) 195, 374, 1162, 1166, 1183, 1448, 2582
FspAI RTGCGCAY 2 cut(s) 1005, 2646
FspBI CTAG 6 cut(s) 483, 854, 899, 1506, 1538, 2150
FspI TGCGCA 3 cut(s) 759, 1005, 2646
GlaI GCGC 7 cut(s) 224, 510, 585, 759, 858, 1005, 2646
GsaI CCCAGC 2 cut(s) 2075, 2127
GsuI CTGGAG 1 cut(s) 617
HaeII RGCGCY 1 cut(s) 860
HaeIII GGCC 8 cut(s) 31, 67, 1395, 1622, 1962, 2156, 2273, 2517
HapII CCGG 5 cut(s) 32, 68, 212, 408, 689
HgaI GACGC 1 cut(s) 1787
HhaI GCGC 7 cut(s) 225, 511, 586, 760, 859, 1006, 2647
Hin6I GCGC 7 cut(s) 223, 509, 584, 758, 857, 1004, 2645
HinP1I GCGC 7 cut(s) 223, 509, 584, 758, 857, 1004, 2645
HincII GTYRAC 2 cut(s) 1500, 1822
HindII GTYRAC 2 cut(s) 1500, 1822
HindIII AAGCTT 2 cut(s) 668, 1207
HinfI GANTC 8 cut(s) 149, 205, 569, 601, 1334, 1501, 2379, 2446
HpaI GTTAAC 1 cut(s) 1822
HpaII CCGG 5 cut(s) 32, 68, 212, 408, 689
HphI GGTGA 3 cut(s) 251, 409, 1616
Hpy166II GTNNAC 6 cut(s) 241, 1318, 1357, 1500, 1676, 1822
Hpy8I GTNNAC 6 cut(s) 241, 1318, 1357, 1500, 1676, 1822
HpyAV CCTTC 9 cut(s) 176, 184, 553, 899, 1120, 1140, 1306, 1420, 1592
HpyCH4III ACNGT 8 cut(s) 25, 238, 253, 751, 1580, 1826, 2140, 2161
HpyCH4IV ACGT 1 cut(s) 2265
HpyF10VI GCNNNNNNNGC 8 cut(s) 517, 592, 1172, 1984, 1996, 2180, 2426, 2604
HpyF3I CTNAG 3 cut(s) 356, 1803, 1947
HpySE526I ACGT 1 cut(s) 2265
HspAI GCGC 7 cut(s) 223, 509, 584, 758, 857, 1004, 2645
Kpn2I TCCGGA 1 cut(s) 211
Ksp22I TGATCA 2 cut(s) 474, 2043
KspAI GTTAAC 1 cut(s) 1822
Kzo9I GATC 8 cut(s) 34, 70, 103, 474, 1243, 2043, 2079, 2083
LguI GCTCTTC 1 cut(s) 635
LweI GCATC 4 cut(s) 352, 942, 2460, 2560
MaeI CTAG 6 cut(s) 483, 854, 899, 1506, 1538, 2150
MaeII ACGT 1 cut(s) 2265
MaeIII GTNAC 5 cut(s) 650, 1131, 1826, 2006, 2195
MalI GATC 8 cut(s) 36, 72, 105, 476, 1245, 2045, 2081, 2085
MbiI CCGCTC 2 cut(s) 628, 1163
MboI GATC 8 cut(s) 34, 70, 103, 474, 1243, 2043, 2079, 2083
MfeI CAATTG 1 cut(s) 1466
MflI RGATCY 1 cut(s) 34
MlsI TGGCCA 2 cut(s) 2156, 2273
MluNI TGGCCA 2 cut(s) 2156, 2273
MlyI GAGTC 2 cut(s) 595, 1495
MmeI TCCRAC 1 cut(s) 1461
Mox20I TGGCCA 2 cut(s) 2156, 2273
Mph1103I ATGCAT 1 cut(s) 1289
MroI TCCGGA 1 cut(s) 211
MscI TGGCCA 2 cut(s) 2156, 2273
MslI CAYNNNNRTG 4 cut(s) 720, 1013, 2104, 2552
Msp20I TGGCCA 2 cut(s) 2156, 2273
MspA1I CMGCKG 3 cut(s) 517, 556, 2053
MspI CCGG 5 cut(s) 32, 68, 212, 408, 689
MspR9I CCNGG 3 cut(s) 1910, 2190, 2575
MunI CAATTG 1 cut(s) 1466
Mva1269I GAATGC 2 cut(s) 2300, 2355
MvaI CCWGG 3 cut(s) 1910, 2190, 2575
MvnI CGCG 2 cut(s) 225, 1619
MwoI GCNNNNNNNGC 8 cut(s) 517, 592, 1172, 1984, 1996, 2180, 2426, 2604
NcoI CCATGG 2 cut(s) 1968, 2400
NdeI CATATG 1 cut(s) 1361
NdeII GATC 8 cut(s) 34, 70, 103, 474, 1243, 2043, 2079, 2083
NheI GCTAGC 1 cut(s) 898
NlaIV GGNNCC 1 cut(s) 36
NmeAIII GCCGAG 3 cut(s) 43, 76, 1837
NmuCI GTSAC 2 cut(s) 650, 1131
NotI GCGGCCGC 1 cut(s) 1620
NsbI TGCGCA 3 cut(s) 759, 1005, 2646
NsiI ATGCAT 1 cut(s) 1289
NspI RCATGY 4 cut(s) 1043, 1287, 1820, 2557
NspV TTCGAA 1 cut(s) 1845
PaeI GCATGC 1 cut(s) 1287
PaeR7I CTCGAG 1 cut(s) 2330
PagI TCATGA 2 cut(s) 471, 2370
PciI ACATGT 1 cut(s) 2553
PciSI GCTCTTC 1 cut(s) 635
PctI GAATGC 2 cut(s) 2300, 2355
PfeI GAWTC 6 cut(s) 149, 205, 569, 1334, 2379, 2446
PflFI GACNNNGTC 2 cut(s) 662, 2470
PflMI CCANNNNNTGG 1 cut(s) 1377
PleI GAGTC 2 cut(s) 595, 1495
PpsI GAGTC 2 cut(s) 595, 1495
PscI ACATGT 1 cut(s) 2553
PshBI ATTAAT 3 cut(s) 1238, 2624, 2640
Psp6I CCWGG 3 cut(s) 1908, 2188, 2573
PspFI CCCAGC 2 cut(s) 2071, 2123
PspGI CCWGG 3 cut(s) 1908, 2188, 2573
PspN4I GGNNCC 1 cut(s) 36
PspPI GGNCC 5 cut(s) 1394, 1527, 1961, 2314, 2466
PsuI RGATCY 1 cut(s) 34
PsyI GACNNNGTC 2 cut(s) 662, 2470
PvuII CAGCTG 1 cut(s) 517
RsaI GTAC 8 cut(s) 8, 374, 431, 481, 490, 974, 1582, 2336
RsaNI GTAC 8 cut(s) 7, 373, 430, 480, 489, 973, 1581, 2335
RseI CAYNNNNRTG 4 cut(s) 720, 1013, 2104, 2552
SapI GCTCTTC 1 cut(s) 635
Sau3AI GATC 8 cut(s) 34, 70, 103, 474, 1243, 2043, 2079, 2083
Sau96I GGNCC 5 cut(s) 1394, 1527, 1961, 2314, 2466
ScaI AGTACT 2 cut(s) 481, 490
SchI GAGTC 2 cut(s) 595, 1495
ScrFI CCNGG 3 cut(s) 1910, 2190, 2575
SfaNI GCATC 4 cut(s) 352, 942, 2460, 2560
SfcI CTRYAG 1 cut(s) 1935
Sfr274I CTCGAG 1 cut(s) 2330
SfuI TTCGAA 1 cut(s) 1845
SinI GGWCC 3 cut(s) 1527, 2314, 2466
SlaI CTCGAG 1 cut(s) 2330
SmiMI CAYNNNNRTG 4 cut(s) 720, 1013, 2104, 2552
SmlI CTYRAG 3 cut(s) 863, 1790, 2330
SmoI CTYRAG 3 cut(s) 863, 1790, 2330
SpeI ACTAGT 1 cut(s) 482
SphI GCATGC 1 cut(s) 1287
SspMI CTAG 6 cut(s) 483, 854, 899, 1506, 1538, 2150
StyD4I CCNGG 3 cut(s) 1908, 2188, 2573
StyI CCWWGG 2 cut(s) 1968, 2400
TaaI ACNGT 8 cut(s) 25, 238, 253, 751, 1580, 1826, 2140, 2161
TaiI ACGT 1 cut(s) 2268
TaqI TCGA 9 cut(s) 201, 306, 1246, 1700, 1845, 2082, 2226, 2331, 2454
TaqII GACCGA 2 cut(s) 638, 2302
TatI WGTACW 2 cut(s) 479, 488
TauI GCSGC 9 cut(s) 269, 425, 514, 592, 641, 1166, 1622, 1625, 2177
TfiI GAWTC 6 cut(s) 149, 205, 569, 1334, 2379, 2446
TscAI CASTG 4 cut(s) 243, 256, 1947, 2164
TseFI GTSAC 2 cut(s) 650, 1131
Tsp45I GTSAC 2 cut(s) 650, 1131
TspDTI ATGAA 8 cut(s) 56, 182, 243, 731, 1083, 1314, 1325, 1611
TspGWI ACGGA 3 cut(s) 390, 724, 1182
TspRI CASTG 4 cut(s) 243, 256, 1947, 2164
Tth111I GACNNNGTC 2 cut(s) 662, 2470
Van91I CCANNNNNTGG 1 cut(s) 1377
VpaK11BI GGWCC 3 cut(s) 1527, 2314, 2466
VspI ATTAAT 3 cut(s) 1238, 2624, 2640
XagI CCTNNNNNAGG 1 cut(s) 1118
XapI RAATTY 4 cut(s) 928, 1223, 1445, 1721
XceI RCATGY 4 cut(s) 1043, 1287, 1820, 2557
XhoI CTCGAG 1 cut(s) 2330
XmiI GTMKAC 1 cut(s) 1356
XspI CTAG 6 cut(s) 483, 854, 899, 1506, 1538, 2150
ZrmI AGTACT 2 cut(s) 481, 490
Zsp2I ATGCAT 1 cut(s) 1289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.