Rw5G001460

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000724
Physical Location & Seq
Forward (+)
1234146 .. 1236901
2756 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G001460.1

Sequence Viewer

Length: 2103 bp
ATGGAAGAAATACGTACTTCCGAACAATCGAAACCCCTCTATGAAAGCCTTAGAGACGGTGATGTATTAGAAGTGGTGAAAGCTTATGCAAAAGCTACTACTCTTGATGCAAGATTAAAGCTAATTCCAACAGTGCACGACGACACAATCCTCCATATGGCGATTTACATGGAGCAAGAAAGCATCGCAATAGGAATCATCGAAATAAGCAGGCATAATGACGAGCTAATCACACAACAAAATGAGTTTGGAAACACTGCTCTTCATGAGGCTGCAGCAATTGATTGTGTACGTGTTGTGGAGAAGTTGTTGGAAAAGGCACCGGCATTGCTGTCCATTCCAAACGAGAATGGGGAAATGCCTCTCTATACAGCAGCTCACTTTGGCCAGAAAAAAAATTTCATTTTGCTGGCTGAAAAACTGAAAAACGATCAACATGCTCTTCATCCCCATTTGTTCTCCCTGTATCCTTCTAACGACCCTGTTACAAAAAGTGGGAAGTACTCTTACGACCCTGAACGCATACGCGGTACCATTCTTCATGCTGCAATACATGCCGAGTTCTTTGAGCTTGCACTTTTGATAGCTAGAAAGCCGGAGTATGCAAAACTTGTTCTACTAAAATACAAAAATTTGAAAACGCCTCTTCAGCTCCTCTCATCCAATTCTTCAGCATTCAAGAGTGGAATGAAATGTAGACTGATCAAGAGGTTCATTTACAACTGTGCTCAATACGAAGATGCTAAAGAGGAAGATGTTCATGAACAAAATTCCAGTGATGATAGTCATGTACAAAGTTCCAGTAGAGCCACTCTGTTTCTACGGCGAATCATGTCTTCAGTTAATAGATCAGTTTGGATTGTTCTGCGAGAATGGCCAACGATGAAGAAGATTCATGACGAAAGGAAAAGGCATGAACTTGCTTTAGAACTAGGCAAGTTTTTAATTGCAGAAGACTTTTCATGGCACATCAAAGCGCCATCCCAGTCAGAGACTCCAGATATCCCATTGTTCATTGCAACAAGCAATGGAATATTAGAAATTGTGGAGGAGACACTTAGAGTCTACCCTTTGTCACTTGAGTATACAAACAAAGAGAAGCAAAACATACTGCACCTCGCTATATTGCACCGTCAGCCGGACATCTTTGATGTGACGAAGAGGTTTGATGTGACGAAGAGGTTAATGCCAAGGTTGGCTCGACAAACCGATAAGAATGGATTCACAATACTGCACCATGCTGGAAACATGAAATTTTATAAAGGAGGCACCCAGCCTGGTCCTGCACTTCAGTTGCAAGAAGAGTTAGAATGGTATGAGAAGGTGGAGAAGTTAGTCCCACGATATTATGCAAGGAATCCTGCTGTTGAACAAAAAGAGACTCTCGAAGAGTTCTGTAGACGAAAATATACGGAAACTGTAGAGGAGTTGTCTCAGAACGCGAATAAGATAATTCCACCCAAATCTGTAAATAAGCTTGGCTGGGACGAAAGCAAAGAGGAGACCCCCAGAGAGTACTTTGCTCGAACCCATGGGGAGCTCCTCGAGGAAGCACAAGAATGGCTAGAGCGGACCTCCAACTCGTGCTCCTTGGTGGCCGGACTAATTGCCTCCGTCGCCTTCGCGGCCGCTTTCACCATCCCGGGCGGCAACAACGCAAGAAACGGCCAGCCCATCCTTATCGACAGCCCTTTCTTCTTGGTCTTCATCATCACTAACGCTCTCTCCATCGCCAGCTCGCTGACGTCACTCGCCATGTTCCTCTCCATCCTGTCGTCACCGTACGAGCTCGAGGACTTCCACCGCTCTCTCCCTCGGAAGCTCATCCTCGGCTTTACCTCCCTCTTCTTCTCGGTGGCCGTCACCATGCTCGCCTTTGCCTCCACAATGATGCTGACAATTCCGATGAAGAAGCGCCTGACTACAACTCTTATATACTGCGTTGCGGTTCTTCCGGTCACCATGTTTGCGCTGTTGCAGTACCCGTTGTGGGAGGCCTTAATAAGGACTGGAAAGTCCTCAGTCAAAGTCGTGAAAAGAATTCTTCCATGGCCACTTATCGAAACTGCTTGCTCAAAGGCCACTAAGGCCCACACTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

700

Amino Acids

79.66

Weight (kDa)

7.67

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 52 - 140 9.5e-06 Ankyrin repeats (3 copies)
PGG PF13962 520 - 632 1.5e-25 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1260
AasI GACNNNNNNGTC 1 cut(s) 2014
AatII GACGTC 1 cut(s) 1748
AbsI CCTCGAGG 1 cut(s) 1544
Acc65I GGTACC 1 cut(s) 530
AccB1I GGYRCC 3 cut(s) 319, 530, 1268
AccBSI CCGCTC 2 cut(s) 1570, 1806
AccI GTMKAC 4 cut(s) 697, 1065, 1085, 1399
AccII CGCG 3 cut(s) 528, 1442, 1625
AciI CCGC 7 cut(s) 528, 1570, 1625, 1629, 1647, 1804, 1946
AcoI YGGCCR 7 cut(s) 385, 875, 1596, 1626, 1666, 1857, 2051
AcsI RAATTY 5 cut(s) 397, 631, 769, 1253, 2040
AcuI CTGAAG 4 cut(s) 632, 654, 822, 1274
AcyI GRCGYC 1 cut(s) 1745
AfaI GTAC 8 cut(s) 16, 291, 503, 532, 792, 1517, 1784, 1982
AfiI CCNNNNNNNGG 4 cut(s) 157, 1138, 1990, 2004
AflIII ACRYGT 1 cut(s) 292
AgsI TTSAA 3 cut(s) 637, 679, 1370
AjnI CCWGG 1 cut(s) 1276
Alw21I GWGCWC 5 cut(s) 138, 730, 1542, 1589, 1791
Alw26I GTCTC 6 cut(s) 48, 986, 1046, 1373, 1437, 1496
Alw44I GTGCAC 1 cut(s) 134
Ama87I CYCGRG 3 cut(s) 1544, 1642, 1790
ApaLI GTGCAC 1 cut(s) 134
ApeKI GCWGC 4 cut(s) 272, 275, 374, 545
ApoI RAATTY 5 cut(s) 397, 631, 769, 1253, 2040
Asp700I GAANNNNTTC 2 cut(s) 756, 1220
Asp718I GGTACC 1 cut(s) 530
AspLEI GCGC 3 cut(s) 979, 1917, 1972
AspS9I GGNCC 3 cut(s) 1280, 1572, 2089
AsuC2I CCSGG 2 cut(s) 1643, 1644
AsuHPI GGTGA 6 cut(s) 71, 88, 1627, 1770, 1855, 1951
AvaI CYCGRG 3 cut(s) 1544, 1642, 1790
AvaII GGWCC 2 cut(s) 1280, 1572
BaeGI GKGCMC 1 cut(s) 138
BalI TGGCCA 3 cut(s) 387, 877, 2053
BanI GGYRCC 3 cut(s) 319, 530, 1268
BanII GRGCYC 2 cut(s) 1542, 1791
BarI GAAGNNNNNNTAC 2 cut(s) 491, 523
BauI CACGAG 1 cut(s) 1582
BbsI GAAGAC 3 cut(s) 828, 960, 1696
Bbv12I GWGCWC 5 cut(s) 138, 730, 1542, 1589, 1791
BbvI GCAGC 4 cut(s) 259, 287, 386, 532
BccI CCATC 5 cut(s) 988, 1646, 1682, 1736, 1775
BceAI ACGGC 3 cut(s) 839, 1681, 1844
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BciT130I CCWGG 1 cut(s) 1278
BciVI GTATCC 1 cut(s) 477
BclI TGATCA 1 cut(s) 702
BcnI CCSGG 2 cut(s) 1643, 1644
BcoDI GTCTC 6 cut(s) 48, 986, 1046, 1373, 1437, 1496
BfaI CTAG 3 cut(s) 588, 932, 1565
BfmI CTRYAG 3 cut(s) 273, 1396, 1419
BfoI RGCGCY 2 cut(s) 980, 1918
BfuI GTATCC 1 cut(s) 477
BglI GCCNNNNNGGC 2 cut(s) 1625, 2087
BisI GCNGC 7 cut(s) 273, 276, 375, 546, 1626, 1629, 1648
BlsI GCNGC 7 cut(s) 274, 277, 376, 547, 1627, 1630, 1649
BmcAI AGTACT 2 cut(s) 503, 1517
Bme1390I CCNGG 3 cut(s) 1278, 1643, 1644
Bme18I GGWCC 2 cut(s) 1280, 1572
BmeT110I CYCGRG 3 cut(s) 1544, 1642, 1790
BmgT120I GGNCC 3 cut(s) 1280, 1572, 2089
BmiI GGNNCC 3 cut(s) 321, 532, 1270
BmrFI CCNGG 3 cut(s) 1278, 1643, 1644
BmrI ACTGGG 1 cut(s) 979
BmsI GCATC 4 cut(s) 97, 192, 730, 1881
BmuI ACTGGG 1 cut(s) 979
BpiI GAAGAC 3 cut(s) 828, 960, 1696
BplI GAGNNNNNCTC 2 cut(s) 1559, 1591
BpmI CTGGAG 1 cut(s) 981
BpuEI CTTGAG 1 cut(s) 1100
BpuMI CCSGG 2 cut(s) 1643, 1644
BsaAI YACGTR 2 cut(s) 14, 293
BsaHI GRCGYC 1 cut(s) 1745
BsaI GGTCTC 1 cut(s) 1496
BsaJI CCNNGG 7 cut(s) 1190, 1531, 1590, 1642, 1814, 1828, 2048
BsaWI WCCGGW 1 cut(s) 1954
BsaXI ACNNNNNCTCC 6 cut(s) 293, 323, 1571, 1601, 1709, 1739
Bsc4I CCNNNNNNNGG 4 cut(s) 157, 1138, 1990, 2004
Bse118I RCCGGY 1 cut(s) 322
Bse1I ACTGG 4 cut(s) 774, 801, 985, 2014
Bse3DI GCAATG 3 cut(s) 326, 1014, 1033
BseBI CCWGG 1 cut(s) 1278
BseDI CCNNGG 7 cut(s) 1190, 1531, 1590, 1642, 1814, 1828, 2048
BseGI GGATG 7 cut(s) 445, 659, 980, 1638, 1674, 1767, 1824
BseLI CCNNNNNNNGG 4 cut(s) 157, 1138, 1990, 2004
BseMI GCAATG 3 cut(s) 326, 1014, 1033
BseMII CTCAG 2 cut(s) 1448, 2034
BseNI ACTGG 4 cut(s) 774, 801, 985, 2014
BseRI GAGGAG 5 cut(s) 644, 1064, 1439, 1514, 1532
BseSI GKGCMC 1 cut(s) 138
BseX3I CGGCCG 1 cut(s) 1626
BseXI GCAGC 4 cut(s) 259, 287, 386, 532
BseYI CCCAGC 2 cut(s) 1272, 1482
BsgI GTGCAG 3 cut(s) 1097, 1217, 1269
Bsh1236I CGCG 3 cut(s) 528, 1442, 1625
Bsh1285I CGRYCG 1 cut(s) 1629
BshNI GGYRCC 3 cut(s) 319, 530, 1268
BsiEI CGRYCG 1 cut(s) 1629
BsiHKAI GWGCWC 5 cut(s) 138, 730, 1542, 1589, 1791
BsiHKCI CYCGRG 3 cut(s) 1544, 1642, 1790
BsiSI CCGG 6 cut(s) 323, 596, 1139, 1599, 1643, 1955
BsiWI CGTACG 1 cut(s) 1782
BslFI GGGAC 2 cut(s) 1322, 1499
BslI CCNNNNNNNGG 4 cut(s) 157, 1138, 1990, 2004
BsmAI GTCTC 6 cut(s) 48, 986, 1046, 1373, 1437, 1496
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 2 cut(s) 1322, 1499
BsmI GAATGC 1 cut(s) 674
Bso31I GGTCTC 1 cut(s) 1496
BsoBI CYCGRG 3 cut(s) 1544, 1642, 1790
Bsp1286I GDGCHC 5 cut(s) 138, 730, 1542, 1589, 1791
Bsp1407I TGTACA 1 cut(s) 790
Bsp143I GATC 3 cut(s) 430, 702, 848
Bsp19I CCATGG 2 cut(s) 1531, 2048
BspACI CCGC 7 cut(s) 528, 1570, 1625, 1629, 1647, 1804, 1946
BspCNI CTCAG 2 cut(s) 1447, 2033
BspFNI CGCG 3 cut(s) 528, 1442, 1625
BspHI TCATGA 4 cut(s) 265, 760, 895, 2099
BspLI GGNNCC 3 cut(s) 321, 532, 1270
BspMAI CTGCAG 1 cut(s) 277
BspQI GCTCTTC 2 cut(s) 267, 447
BspT107I GGYRCC 3 cut(s) 319, 530, 1268
BspTNI GGTCTC 1 cut(s) 1496
BsrBI CCGCTC 2 cut(s) 1570, 1806
BsrDI GCAATG 3 cut(s) 326, 1014, 1033
BsrFI RCCGGY 1 cut(s) 322
BsrGI TGTACA 1 cut(s) 790
BsrI ACTGG 4 cut(s) 774, 801, 985, 2014
BssAI RCCGGY 1 cut(s) 322
BssECI CCNNGG 7 cut(s) 1190, 1531, 1590, 1642, 1814, 1828, 2048
BssMI GATC 3 cut(s) 430, 702, 848
BssNAI GTATAC 1 cut(s) 1086
BssNI GRCGYC 1 cut(s) 1745
BssSI CACGAG 1 cut(s) 1582
BssT1I CCWWGG 4 cut(s) 1190, 1531, 1590, 2048
Bst1107I GTATAC 1 cut(s) 1086
Bst2BI CACGAG 1 cut(s) 1582
Bst2UI CCWGG 1 cut(s) 1278
Bst4CI ACNGT 6 cut(s) 59, 133, 725, 1133, 1420, 1782
Bst6I CTCTTC 8 cut(s) 267, 447, 651, 1154, 1172, 1296, 1383, 1850
BstACI GRCGYC 1 cut(s) 1745
BstAPI GCANNNNNTGC 1 cut(s) 554
BstAUI TGTACA 1 cut(s) 790
BstBAI YACGTR 2 cut(s) 14, 293
BstC8I GCNNGC 8 cut(s) 212, 411, 573, 1670, 1735, 1739, 1872, 2071
BstDEI CTNAG 5 cut(s) 50, 1058, 1434, 2020, 2085
BstDSI CCRYGG 2 cut(s) 1531, 2048
BstEII GGTNACC 1 cut(s) 1957
BstENI CCTNNNNNAGG 1 cut(s) 2002
BstF5I GGATG 7 cut(s) 445, 659, 980, 1638, 1674, 1767, 1824
BstFNI CGCG 3 cut(s) 528, 1442, 1625
BstH2I RGCGCY 2 cut(s) 980, 1918
BstHHI GCGC 3 cut(s) 979, 1917, 1972
BstKTI GATC 3 cut(s) 433, 705, 851
BstMAI GTCTC 6 cut(s) 48, 986, 1046, 1373, 1437, 1496
BstMBI GATC 3 cut(s) 430, 702, 848
BstMCI CGRYCG 1 cut(s) 1629
BstMWI GCNNNNNNNGC 7 cut(s) 554, 649, 874, 1135, 1616, 1625, 2087
BstNI CCWGG 1 cut(s) 1278
BstNSI RCATGY 2 cut(s) 440, 557
BstPI GGTNACC 1 cut(s) 1957
BstSCI CCNGG 3 cut(s) 1276, 1641, 1642
BstSFI CTRYAG 3 cut(s) 273, 1396, 1419
BstSLI GKGCMC 1 cut(s) 138
BstSNI TACGTA 1 cut(s) 14
BstUI CGCG 3 cut(s) 528, 1442, 1625
BstV1I GCAGC 4 cut(s) 259, 287, 386, 532
BstV2I GAAGAC 3 cut(s) 828, 960, 1696
BstZ17I GTATAC 1 cut(s) 1086
BstZI CGGCCG 1 cut(s) 1626
BsuI GTATCC 1 cut(s) 477
BtgI CCRYGG 2 cut(s) 1531, 2048
BtgZI GCGATG 2 cut(s) 169, 1714
BtsCI GGATG 7 cut(s) 445, 659, 980, 1638, 1674, 1767, 1824
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 3 cut(s) 138, 255, 781
Cac8I GCNNGC 8 cut(s) 212, 411, 573, 1670, 1735, 1739, 1872, 2071
CciI TCATGA 4 cut(s) 265, 760, 895, 2099
CciNI GCGGCCGC 1 cut(s) 1626
CfoI GCGC 3 cut(s) 979, 1917, 1972
Cfr10I RCCGGY 1 cut(s) 322
Cfr13I GGNCC 3 cut(s) 1280, 1572, 2089
Cfr9I CCCGGG 1 cut(s) 1642
Csp6I GTAC 8 cut(s) 15, 290, 502, 531, 791, 1516, 1783, 1981
CviQI GTAC 8 cut(s) 15, 290, 502, 531, 791, 1516, 1783, 1981
DdeI CTNAG 5 cut(s) 50, 1058, 1434, 2020, 2085
DpnI GATC 3 cut(s) 432, 704, 850
DpnII GATC 3 cut(s) 430, 702, 848
DrdI GACNNNNNNGTC 1 cut(s) 2014
DseDI GACNNNNNNGTC 1 cut(s) 2014
EaeI YGGCCR 7 cut(s) 385, 875, 1596, 1626, 1666, 1857, 2051
EagI CGGCCG 1 cut(s) 1626
Eam1104I CTCTTC 8 cut(s) 267, 447, 651, 1154, 1172, 1296, 1383, 1850
EarI CTCTTC 8 cut(s) 267, 447, 651, 1154, 1172, 1296, 1383, 1850
Ecl136II GAGCTC 2 cut(s) 1540, 1789
EclXI CGGCCG 1 cut(s) 1626
Eco105I TACGTA 1 cut(s) 14
Eco130I CCWWGG 4 cut(s) 1190, 1531, 1590, 2048
Eco147I AGGCCT 1 cut(s) 1997
Eco24I GRGCYC 2 cut(s) 1542, 1791
Eco31I GGTCTC 1 cut(s) 1496
Eco32I GATATC 1 cut(s) 1003
Eco47I GGWCC 2 cut(s) 1280, 1572
Eco52I CGGCCG 1 cut(s) 1626
Eco53kI GAGCTC 2 cut(s) 1540, 1789
Eco57I CTGAAG 4 cut(s) 632, 654, 822, 1274
Eco88I CYCGRG 3 cut(s) 1544, 1642, 1790
Eco91I GGTNACC 1 cut(s) 1957
EcoICRI GAGCTC 2 cut(s) 1540, 1789
EcoNI CCTNNNNNAGG 1 cut(s) 2002
EcoO65I GGTNACC 1 cut(s) 1957
EcoRI GAATTC 1 cut(s) 2040
EcoRII CCWGG 1 cut(s) 1276
EcoRV GATATC 1 cut(s) 1003
EcoT14I CCWWGG 4 cut(s) 1190, 1531, 1590, 2048
EcoT38I GRGCYC 2 cut(s) 1542, 1791
ErhI CCWWGG 4 cut(s) 1190, 1531, 1590, 2048
Esp3I CGTCTC 1 cut(s) 48
FaqI GGGAC 2 cut(s) 1322, 1499
FauNDI CATATG 1 cut(s) 156
FbaI TGATCA 1 cut(s) 702
FblI GTMKAC 4 cut(s) 697, 1065, 1085, 1399
Fnu4HI GCNGC 7 cut(s) 273, 276, 375, 546, 1626, 1629, 1648
FokI GGATG 7 cut(s) 432, 646, 967, 1625, 1661, 1754, 1811
FriOI GRGCYC 2 cut(s) 1542, 1791
Fsp4HI GCNGC 7 cut(s) 273, 276, 375, 546, 1626, 1629, 1648
FspBI CTAG 3 cut(s) 588, 932, 1565
GlaI GCGC 3 cut(s) 978, 1916, 1971
GluI GCNGC 7 cut(s) 273, 276, 375, 546, 1626, 1629, 1648
GsaI CCCAGC 2 cut(s) 1276, 1486
GsuI CTGGAG 1 cut(s) 981
HaeII RGCGCY 2 cut(s) 980, 1918
HapII CCGG 6 cut(s) 323, 596, 1139, 1599, 1643, 1955
HhaI GCGC 3 cut(s) 979, 1917, 1972
Hin1I GRCGYC 1 cut(s) 1745
Hin6I GCGC 3 cut(s) 977, 1915, 1970
HinP1I GCGC 3 cut(s) 977, 1915, 1970
HindIII AAGCTT 2 cut(s) 81, 1475
HinfI GANTC 8 cut(s) 195, 828, 892, 994, 1062, 1221, 1357, 1381
HpaII CCGG 6 cut(s) 323, 596, 1139, 1599, 1643, 1955
HphI GGTGA 6 cut(s) 71, 88, 1627, 1770, 1855, 1951
Hpy166II GTNNAC 6 cut(s) 136, 290, 698, 1066, 1086, 1400
Hpy188I TCNGA 5 cut(s) 22, 991, 1437, 1818, 1905
Hpy8I GTNNAC 6 cut(s) 136, 290, 698, 1066, 1086, 1400
Hpy99I CGWCG 2 cut(s) 143, 1619
HpyAV CCTTC 3 cut(s) 480, 1315, 1630
HpyCH4III ACNGT 6 cut(s) 59, 133, 725, 1133, 1420, 1782
HpyCH4IV ACGT 3 cut(s) 13, 292, 1745
HpyF10VI GCNNNNNNNGC 7 cut(s) 554, 649, 874, 1135, 1616, 1625, 2087
HpyF3I CTNAG 5 cut(s) 50, 1058, 1434, 2020, 2085
HpySE526I ACGT 3 cut(s) 13, 292, 1745
Hsp92I GRCGYC 1 cut(s) 1745
HspAI GCGC 3 cut(s) 977, 1915, 1970
KpnI GGTACC 1 cut(s) 534
Ksp22I TGATCA 1 cut(s) 702
Kzo9I GATC 3 cut(s) 430, 702, 848
LguI GCTCTTC 2 cut(s) 267, 447
LmnI GCTCC 5 cut(s) 172, 657, 1537, 1545, 1592
Lsp1109I GCAGC 4 cut(s) 259, 287, 386, 532
LweI GCATC 4 cut(s) 97, 192, 730, 1881
MaeI CTAG 3 cut(s) 588, 932, 1565
MaeII ACGT 3 cut(s) 13, 292, 1745
MaeIII GTNAC 8 cut(s) 484, 1074, 1153, 1171, 1746, 1776, 1861, 1957
MalI GATC 3 cut(s) 432, 704, 850
MbiI CCGCTC 2 cut(s) 1570, 1806
MboI GATC 3 cut(s) 430, 702, 848
MfeI CAATTG 1 cut(s) 279
MhlI GDGCHC 5 cut(s) 138, 730, 1542, 1589, 1791
MlsI TGGCCA 3 cut(s) 387, 877, 2053
MluNI TGGCCA 3 cut(s) 387, 877, 2053
MlyI GAGTC 3 cut(s) 988, 1071, 1375
MmeI TCCRAC 3 cut(s) 152, 291, 1602
Mox20I TGGCCA 3 cut(s) 387, 877, 2053
MroXI GAANNNNTTC 2 cut(s) 756, 1220
MscI TGGCCA 3 cut(s) 387, 877, 2053
MseI TTAA 5 cut(s) 116, 843, 944, 1184, 2000
MslI CAYNNNNRTG 2 cut(s) 1558, 1889
Msp20I TGGCCA 3 cut(s) 387, 877, 2053
MspI CCGG 6 cut(s) 323, 596, 1139, 1599, 1643, 1955
MspR9I CCNGG 3 cut(s) 1278, 1643, 1644
MunI CAATTG 1 cut(s) 279
Mva1269I GAATGC 1 cut(s) 674
MvaI CCWGG 1 cut(s) 1278
MvnI CGCG 3 cut(s) 528, 1442, 1625
MwoI GCNNNNNNNGC 7 cut(s) 554, 649, 874, 1135, 1616, 1625, 2087
NciI CCSGG 2 cut(s) 1643, 1644
NcoI CCATGG 2 cut(s) 1531, 2048
NdeI CATATG 1 cut(s) 156
NdeII GATC 3 cut(s) 430, 702, 848
NlaIV GGNNCC 3 cut(s) 321, 532, 1270
NmeAIII GCCGAG 2 cut(s) 583, 1809
NmuCI GTSAC 7 cut(s) 1074, 1153, 1171, 1746, 1776, 1861, 1957
NotI GCGGCCGC 1 cut(s) 1626
NspI RCATGY 2 cut(s) 440, 557
PaeR7I CTCGAG 2 cut(s) 1544, 1790
PagI TCATGA 4 cut(s) 265, 760, 895, 2099
PceI AGGCCT 1 cut(s) 1997
PciSI GCTCTTC 2 cut(s) 267, 447
PctI GAATGC 1 cut(s) 674
PdmI GAANNNNTTC 2 cut(s) 756, 1220
PfeI GAWTC 5 cut(s) 195, 828, 892, 1221, 1357
Pfl23II CGTACG 1 cut(s) 1782
PkrI GCNGC 7 cut(s) 274, 277, 376, 547, 1627, 1630, 1649
PleI GAGTC 3 cut(s) 988, 1070, 1375
PpsI GAGTC 3 cut(s) 988, 1070, 1375
Ppu21I YACGTR 2 cut(s) 14, 293
PsiI TTATAA 1 cut(s) 1260
Psp124BI GAGCTC 2 cut(s) 1542, 1791
Psp6I CCWGG 1 cut(s) 1276
PspEI GGTNACC 1 cut(s) 1957
PspFI CCCAGC 2 cut(s) 1272, 1482
PspGI CCWGG 1 cut(s) 1276
PspLI CGTACG 1 cut(s) 1782
PspN4I GGNNCC 3 cut(s) 321, 532, 1270
PspPI GGNCC 3 cut(s) 1280, 1572, 2089
PspXI VCTCGAGB 2 cut(s) 1544, 1790
PstI CTGCAG 1 cut(s) 277
RsaI GTAC 8 cut(s) 16, 291, 503, 532, 792, 1517, 1784, 1982
RsaNI GTAC 8 cut(s) 15, 290, 502, 531, 791, 1516, 1783, 1981
RseI CAYNNNNRTG 2 cut(s) 1558, 1889
SacI GAGCTC 2 cut(s) 1542, 1791
SapI GCTCTTC 2 cut(s) 267, 447
SaqAI TTAA 5 cut(s) 116, 843, 944, 1184, 2000
SatI GCNGC 7 cut(s) 273, 276, 375, 546, 1626, 1629, 1648
Sau3AI GATC 3 cut(s) 430, 702, 848
Sau96I GGNCC 3 cut(s) 1280, 1572, 2089
ScaI AGTACT 2 cut(s) 503, 1517
SchI GAGTC 3 cut(s) 988, 1071, 1375
ScrFI CCNGG 3 cut(s) 1278, 1643, 1644
SduI GDGCHC 5 cut(s) 138, 730, 1542, 1589, 1791
SfaNI GCATC 4 cut(s) 97, 192, 730, 1881
SfcI CTRYAG 3 cut(s) 273, 1396, 1419
SfiI GGCCNNNNNGGCC 1 cut(s) 2087
Sfr274I CTCGAG 2 cut(s) 1544, 1790
SinI GGWCC 2 cut(s) 1280, 1572
SlaI CTCGAG 2 cut(s) 1544, 1790
SmaI CCCGGG 1 cut(s) 1644
SmiMI CAYNNNNRTG 2 cut(s) 1558, 1889
SmlI CTYRAG 3 cut(s) 1079, 1544, 1790
SmoI CTYRAG 3 cut(s) 1079, 1544, 1790
SnaBI TACGTA 1 cut(s) 14
SseBI AGGCCT 1 cut(s) 1997
SsiI CCGC 7 cut(s) 528, 1570, 1625, 1629, 1647, 1804, 1946
SspI AATATT 1 cut(s) 1035
SspMI CTAG 3 cut(s) 588, 932, 1565
SstI GAGCTC 2 cut(s) 1542, 1791
StuI AGGCCT 1 cut(s) 1997
StyD4I CCNGG 3 cut(s) 1276, 1641, 1642
StyI CCWWGG 4 cut(s) 1190, 1531, 1590, 2048
TaaI ACNGT 6 cut(s) 59, 133, 725, 1133, 1420, 1782
TaiI ACGT 3 cut(s) 16, 295, 1748
TaqI TCGA 9 cut(s) 29, 201, 1201, 1386, 1525, 1545, 1683, 1791, 2061
TatI WGTACW 3 cut(s) 501, 790, 1515
TauI GCSGC 3 cut(s) 1628, 1631, 1650
TfiI GAWTC 5 cut(s) 195, 828, 892, 1221, 1357
Tru1I TTAA 5 cut(s) 116, 843, 944, 1184, 2000
Tru9I TTAA 5 cut(s) 116, 843, 944, 1184, 2000
TscAI CASTG 3 cut(s) 138, 262, 781
TseFI GTSAC 7 cut(s) 1074, 1153, 1171, 1746, 1776, 1861, 1957
TseI GCWGC 4 cut(s) 272, 275, 374, 545
Tsp45I GTSAC 7 cut(s) 1074, 1153, 1171, 1746, 1776, 1861, 1957
TspGWI ACGGA 2 cut(s) 1427, 1603
TspMI CCCGGG 1 cut(s) 1642
TspRI CASTG 3 cut(s) 138, 262, 781
VneI GTGCAC 1 cut(s) 134
VpaK11BI GGWCC 2 cut(s) 1280, 1572
XagI CCTNNNNNAGG 1 cut(s) 2002
XapI RAATTY 5 cut(s) 397, 631, 769, 1253, 2040
XceI RCATGY 2 cut(s) 440, 557
XhoI CTCGAG 2 cut(s) 1544, 1790
XmaI CCCGGG 1 cut(s) 1642
XmiI GTMKAC 4 cut(s) 697, 1065, 1085, 1399
XmnI GAANNNNTTC 2 cut(s) 756, 1220
XspI CTAG 3 cut(s) 588, 932, 1565
ZraI GACGTC 1 cut(s) 1746
ZrmI AGTACT 2 cut(s) 503, 1517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.