Rw5G001480

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000724
Physical Location & Seq
Forward (+)
1241917 .. 1246118
4202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G001480.1

Sequence Viewer

Length: 2322 bp
ATGGCAGAAAATAGCAGCATAAATCCCAAACACCTATATCAAAGCCTAAAGAATGGTGACAGAGCCAAAGTGATGGAATATTGTTATGATAAAACTAGGTCTGATGATGAAAGGTTGAAGCTCATTCCAACAGTGCACGATGATACAATCCTCCATTTAGCAATTAACATGGGGCAGCAAGACATAGCAAAAGAAATCGTCAAAAGATGTTCCGGGGGTTCTGATGATAACCTACTTATGAAGAAGAATGCCCTCGGAAACACAGTTCTGCACGAGGCTGCAGCAACAAGCATGACAAGCCTTGTGAAGGAGTTGTTAAGAAAGGCACCAGGCTTGTTGTCTATTTCGAACAACAAAAATGAAATGCCTCTCTATACAGCAGCTTACTATGGCCAGACCGACATGTTCAATTTGTTGGCGGACGAAGTAGACAAAAGTCTTGATGTTGATCATCATTTGCGACCCAGGGGTCGTAAGACGAACCCCGATAAAGCGCGGACTACCATTCTTCATGCTGCAATACATGCCGAGTTCTTTGAGCTTTCACTTCTGATAGCAAAAAGGTATACGCAACTAGTTAATAAAAAGGACGAAAATCGGCTGACAGGCCTTCAGCTGCTTTCATGTAATCCTTCAGCCTTCAGGAGTGGAACCAAATATGGACCTATCAAGAGGTTCATTTATTCCTGTGCTCCACATGAGAATGTGATAATGGATAAAAATGACAGCCACGAACAAGAAGAGGCTGATGAATACATTTTCAGGGATCTAGGTGATGAGGAAAATACAGCTTGCTTCAAGATGCCTCAGAGTCGAACATATATGGATTACTGCTGCACCATTACTTTACGGCAAGCCATCTCTTCTGTTAATAGATCGGTTTGGAAAATTCTTCAAGCTTGGGGCACAATGAAGAGGATCCATGGGGACAAGAAGAGGCATGAATCAGCCTTCAAACTAGCCAAGTTACTAATTGCAGCAGACTTTTCATGGGACAGCATAGGAGTGCAAGATGAAATCGCGAGCTCAAATGTCACCGCTGCTACAAATGATGCTCCTCCTTTGGATCCCAAAGCTGATATTGAATTCCCAGAAGCCCGTGCAGAAGCTATAAGTGGTAGCGGTCACCTTTCTGATGATGGAAAGCGAGAAAAAGCACTAGGCTGCAGTGATAGAGGTACTCACCGTGCTGGCCCTAGTGGCGGCCCAGATATGGCAGCTCCATCCATGTCAGAACTTCATTCACCTCCAACGCCATTGCTCCTTGCAACAAGCCACGGAATACTAGAAATTGTGGATGAGATACTTTCTGTCTACCCTCCAGCAGTTGAGAATGTAAGTGAAATGGGGCAAAACATTCTGCATGTAGCCATTATGCACCGCCAGATGGACGTCTTTAAACGAGTCATTAAGATGGAAGCACCCAAGTCAAGGTTGGTTCGACGTATAGACGAGGATGGATTTACAATCTTGCACCATGTTGGAAACATGGATTTTTACAAAGGAGGCACACAGCCTGGTCCTGCACTCCAGTTGCAAGAAGAGTTGGAATGGTTTGAGATTGTGGAGAAGGAAGTTCCACCCCATTATGCGATGCAACTTGCCTGCAACAAAAAGGAACAGAAAAACGAGACTGAACAACTAGTATTTAATTTGCAGCAGATTGTGGCGAAGGAGGTTGCACCCCAGAATGCGATACAACTTGCCTGGAACAAAAAAGAACAGGTAAACGAGACCCCCAAACAGTACTTTCGTCGCACACATGGCGGCCTTCTCAAGGAGGCCCAAGATTGGATAAAGCGGACCGCCGAGTCGTGCTCGACGGTGGCAGGACTAATGGCCACCGTCGCTTTCGCCGCCGCCTTCACCGTCCCTGGAGGCAACGACGAGAAAAGCGGCACTCCACTCCTTCACAGCCCTTTCTTCTTAGTCTTCATCATCACAGACGCTCTCTCCCTAGCCAGCTCGCTGACGTCACTCGTCATGTTCCTGTCGATCCTGACGTCACCGTACGAGATCGACGACTTCCGCTACTCTCTCCCTCGGAAGCTTATCCTCGGCTTCACCTTCCTCTTCTTCTCGGTGGCGGTGACCATGCTGGCCTTTGCTTCCACACTGATGCTGACAATTTCGACAAAGAAGAGACTGACAACAACTTTTATATACTGCGTTGCGTTTCTTCCGGTCACTATGTTCGCTCTCTTGCAGTTCCCATTGTACGTGGCCTTCAAAACGACTCTCCTGTACTCTGTACAGGCCGTCCAAAGCGTTCTTCCGTGGCGTTTCATCTCCGTTGCTTTTTCAAAACTCAAAAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

773

Amino Acids

86.54

Weight (kDa)

6.77

Isoelectric Point (pI)

45.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 51 - 144 1.4e-08 Ankyrin repeats (3 copies)
PGG PF13962 597 - 708 3.2e-24 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 468, 1810
AatII GACGTC 3 cut(s) 1395, 1976, 2006
AccB1I GGYRCC 1 cut(s) 325
AccI GTMKAC 3 cut(s) 429, 566, 1314
AccII CGCG 2 cut(s) 496, 1022
AclWI GGATC 6 cut(s) 774, 913, 926, 1061, 1074, 1990
AcoI YGGCCR 2 cut(s) 391, 1839
AcsI RAATTY 2 cut(s) 888, 1085
AcuI CTGAAG 3 cut(s) 596, 618, 625
AcyI GRCGYC 3 cut(s) 1392, 1973, 2003
AfaI GTAC 6 cut(s) 1180, 1748, 2012, 2219, 2246, 2253
AfiI CCNNNNNNNGG 7 cut(s) 307, 1202, 1213, 1387, 1431, 1777, 1791
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 8 cut(s) 118, 409, 799, 896, 955, 1085, 2230, 2304
AhlI ACTAGT 2 cut(s) 574, 1642
AjnI CCWGG 5 cut(s) 328, 464, 1516, 1706, 1873
AjuI GAANNNNNNNTTGG 2 cut(s) 2256, 2288
AloI GAACNNNNNNTCC 2 cut(s) 249, 281
Alw21I GWGCWC 4 cut(s) 138, 694, 1028, 1820
Alw26I GTCTC 3 cut(s) 1625, 1727, 2137
Alw44I GTGCAC 1 cut(s) 134
AlwI GGATC 6 cut(s) 774, 913, 926, 1061, 1074, 1990
ApaLI GTGCAC 1 cut(s) 134
ApoI RAATTY 2 cut(s) 888, 1085
AspLEI GCGC 1 cut(s) 496
AspS9I GGNCC 6 cut(s) 662, 1193, 1205, 1520, 1783, 1803
AsuC2I CCSGG 1 cut(s) 214
AsuII TTCGAA 1 cut(s) 347
AvaII GGWCC 3 cut(s) 662, 1520, 1803
BaeGI GKGCMC 2 cut(s) 138, 908
BalI TGGCCA 2 cut(s) 393, 1841
BamHI GGATCC 2 cut(s) 918, 1066
BanI GGYRCC 1 cut(s) 325
BanII GRGCYC 1 cut(s) 1028
BarI GAAGNNNNNNTAC 2 cut(s) 2210, 2242
BauI CACGAG 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 1924
Bbv12I GWGCWC 4 cut(s) 138, 694, 1028, 1820
BccI CCATC 7 cut(s) 67, 866, 1133, 1231, 1381, 1408, 1451
BceAI ACGGC 2 cut(s) 866, 2243
BciT130I CCWGG 5 cut(s) 330, 466, 1518, 1708, 1875
BclI TGATCA 1 cut(s) 448
BcnI CCSGG 1 cut(s) 214
BcoDI GTCTC 3 cut(s) 1625, 1727, 2137
BcuI ACTAGT 2 cut(s) 574, 1642
BfaI CTAG 9 cut(s) 96, 575, 770, 959, 1160, 1197, 1286, 1643, 1958
BfmI CTRYAG 2 cut(s) 279, 1165
BglI GCCNNNNNGGC 1 cut(s) 1200
BmcAI AGTACT 1 cut(s) 1748
Bme1390I CCNGG 6 cut(s) 214, 330, 466, 1518, 1708, 1875
Bme18I GGWCC 3 cut(s) 662, 1520, 1803
BmgT120I GGNCC 6 cut(s) 662, 1193, 1205, 1520, 1783, 1803
BmiI GGNNCC 4 cut(s) 327, 652, 920, 1068
BmrFI CCNGG 6 cut(s) 214, 330, 466, 1518, 1708, 1875
BmsI GCATC 4 cut(s) 792, 1042, 1584, 2109
BoxI GACNNNNGTC 1 cut(s) 435
BpiI GAAGAC 1 cut(s) 1924
BplI GAGNNNNNCTC 2 cut(s) 1802, 1834
BpmI CTGGAG 3 cut(s) 1305, 1514, 1896
Bpu14I TTCGAA 1 cut(s) 347
BpuEI CTTGAG 1 cut(s) 1760
BpuMI CCSGG 1 cut(s) 214
BsaAI YACGTR 1 cut(s) 2221
BsaBI GATNNNNATC 1 cut(s) 447
BsaHI GRCGYC 3 cut(s) 1392, 1973, 2003
BsaI GGTCTC 1 cut(s) 1727
BsaWI WCCGGW 1 cut(s) 2182
BsaXI ACNNNNNCTCC 2 cut(s) 1937, 1967
Bsc4I CCNNNNNNNGG 7 cut(s) 307, 1202, 1213, 1387, 1431, 1777, 1791
Bse1I ACTGG 1 cut(s) 1531
Bse3DI GCAATG 1 cut(s) 1256
Bse8I GATNNNNATC 1 cut(s) 447
BseBI CCWGG 5 cut(s) 330, 466, 1518, 1708, 1875
BseGI GGATG 3 cut(s) 1223, 1303, 1462
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 7 cut(s) 307, 1202, 1213, 1387, 1431, 1777, 1791
BseMI GCAATG 1 cut(s) 1256
BseMII CTCAG 1 cut(s) 821
BseNI ACTGG 1 cut(s) 1531
BseRI GAGGAG 1 cut(s) 1047
BseSI GKGCMC 2 cut(s) 138, 908
BsgI GTGCAG 4 cut(s) 254, 820, 1122, 1509
Bsh1236I CGCG 2 cut(s) 496, 1022
BshNI GGYRCC 1 cut(s) 325
BsiHKAI GWGCWC 4 cut(s) 138, 694, 1028, 1820
BsiSI CCGG 2 cut(s) 213, 2183
BsiWI CGTACG 1 cut(s) 2010
BslFI GGGAC 3 cut(s) 941, 1007, 1856
BslI CCNNNNNNNGG 7 cut(s) 307, 1202, 1213, 1387, 1431, 1777, 1791
BsmAI GTCTC 3 cut(s) 1625, 1727, 2137
BsmFI GGGAC 3 cut(s) 941, 1007, 1856
BsmI GAATGC 2 cut(s) 253, 1696
Bso31I GGTCTC 1 cut(s) 1727
Bsp119I TTCGAA 1 cut(s) 347
Bsp1286I GDGCHC 5 cut(s) 138, 694, 908, 1028, 1820
Bsp1407I TGTACA 1 cut(s) 2251
Bsp143I GATC 7 cut(s) 448, 766, 875, 918, 1066, 1995, 2016
Bsp19I CCATGG 1 cut(s) 922
Bsp68I TCGCGA 1 cut(s) 1022
BspCNI CTCAG 1 cut(s) 820
BspFNI CGCG 2 cut(s) 496, 1022
BspLI GGNNCC 4 cut(s) 327, 652, 920, 1068
BspMAI CTGCAG 2 cut(s) 283, 1169
BspPI GGATC 6 cut(s) 774, 913, 926, 1061, 1074, 1990
BspT104I TTCGAA 1 cut(s) 347
BspT107I GGYRCC 1 cut(s) 325
BspTNI GGTCTC 1 cut(s) 1727
BsrDI GCAATG 1 cut(s) 1256
BsrGI TGTACA 1 cut(s) 2251
BsrI ACTGG 1 cut(s) 1531
BssMI GATC 7 cut(s) 448, 766, 875, 918, 1066, 1995, 2016
BssNAI GTATAC 1 cut(s) 567
BssNI GRCGYC 3 cut(s) 1392, 1973, 2003
BssSI CACGAG 1 cut(s) 272
BssT1I CCWWGG 1 cut(s) 922
Bst1107I GTATAC 1 cut(s) 567
Bst2BI CACGAG 1 cut(s) 272
Bst2UI CCWGG 5 cut(s) 330, 466, 1518, 1708, 1875
Bst4CI ACNGT 8 cut(s) 133, 265, 1187, 1746, 1825, 1846, 1870, 2010
Bst6I CTCTTC 7 cut(s) 735, 868, 908, 929, 1536, 2078, 2135
BstACI GRCGYC 3 cut(s) 1392, 1973, 2003
BstAPI GCANNNNNTGC 1 cut(s) 524
BstAUI TGTACA 1 cut(s) 2251
BstBAI YACGTR 1 cut(s) 2221
BstBI TTCGAA 1 cut(s) 347
BstC8I GCNNGC 8 cut(s) 793, 855, 1024, 1192, 1606, 1963, 1967, 2100
BstDEI CTNAG 2 cut(s) 807, 1927
BstDSI CCRYGG 3 cut(s) 922, 1276, 2276
BstEII GGTNACC 2 cut(s) 1124, 2089
BstENI CCTNNNNNAGG 2 cut(s) 305, 1775
BstF5I GGATG 3 cut(s) 1223, 1303, 1462
BstFNI CGCG 2 cut(s) 496, 1022
BstHHI GCGC 1 cut(s) 496
BstKTI GATC 7 cut(s) 451, 769, 878, 921, 1069, 1998, 2019
BstMAI GTCTC 3 cut(s) 1625, 1727, 2137
BstMBI GATC 7 cut(s) 448, 766, 875, 918, 1066, 1995, 2016
BstMWI GCNNNNNNNGC 6 cut(s) 297, 524, 1200, 1764, 1847, 1856
BstNI CCWGG 5 cut(s) 330, 466, 1518, 1708, 1875
BstNSI RCATGY 3 cut(s) 406, 527, 1367
BstPAI GACNNNNGTC 1 cut(s) 435
BstPI GGTNACC 2 cut(s) 1124, 2089
BstSCI CCNGG 6 cut(s) 212, 328, 464, 1516, 1706, 1873
BstSFI CTRYAG 2 cut(s) 279, 1165
BstSLI GKGCMC 2 cut(s) 138, 908
BstUI CGCG 2 cut(s) 496, 1022
BstV2I GAAGAC 1 cut(s) 1924
BstX2I RGATCY 3 cut(s) 766, 918, 1066
BstXI CCANNNNNNTGG 1 cut(s) 73
BstYI RGATCY 3 cut(s) 766, 918, 1066
BstZ17I GTATAC 1 cut(s) 567
BtgI CCRYGG 3 cut(s) 922, 1276, 2276
BtgZI GCGATG 1 cut(s) 1607
BtsCI GGATG 3 cut(s) 1223, 1303, 1462
BtsI GCAGTG 1 cut(s) 1174
BtsIMutI CAGTG 3 cut(s) 138, 1174, 2114
BtuMI TCGCGA 1 cut(s) 1022
Cac8I GCNNGC 8 cut(s) 793, 855, 1024, 1192, 1606, 1963, 1967, 2100
CfoI GCGC 1 cut(s) 496
Cfr13I GGNCC 6 cut(s) 662, 1193, 1205, 1520, 1783, 1803
CpoI CGGWCCG 1 cut(s) 1803
CseI GACGC 1 cut(s) 1955
Csp6I GTAC 6 cut(s) 1179, 1747, 2011, 2218, 2245, 2252
CspI CGGWCCG 1 cut(s) 1803
CviQI GTAC 6 cut(s) 1179, 1747, 2011, 2218, 2245, 2252
DdeI CTNAG 2 cut(s) 807, 1927
DpnI GATC 7 cut(s) 450, 768, 877, 920, 1068, 1997, 2018
DpnII GATC 7 cut(s) 448, 766, 875, 918, 1066, 1995, 2016
DraI TTTAAA 1 cut(s) 1399
DrdI GACNNNNNNGTC 2 cut(s) 468, 1810
DseDI GACNNNNNNGTC 2 cut(s) 468, 1810
EaeI YGGCCR 2 cut(s) 391, 1839
Eam1104I CTCTTC 7 cut(s) 735, 868, 908, 929, 1536, 2078, 2135
EarI CTCTTC 7 cut(s) 735, 868, 908, 929, 1536, 2078, 2135
EciI GGCGGA 1 cut(s) 434
Ecl136II GAGCTC 1 cut(s) 1026
Eco130I CCWWGG 1 cut(s) 922
Eco147I AGGCCT 1 cut(s) 609
Eco24I GRGCYC 1 cut(s) 1028
Eco31I GGTCTC 1 cut(s) 1727
Eco47I GGWCC 3 cut(s) 662, 1520, 1803
Eco53kI GAGCTC 1 cut(s) 1026
Eco57I CTGAAG 3 cut(s) 596, 618, 625
Eco91I GGTNACC 2 cut(s) 1124, 2089
EcoICRI GAGCTC 1 cut(s) 1026
EcoNI CCTNNNNNAGG 2 cut(s) 305, 1775
EcoO65I GGTNACC 2 cut(s) 1124, 2089
EcoRI GAATTC 1 cut(s) 1085
EcoRII CCWGG 5 cut(s) 328, 464, 1516, 1706, 1873
EcoT14I CCWWGG 1 cut(s) 922
EcoT38I GRGCYC 1 cut(s) 1028
ErhI CCWWGG 1 cut(s) 922
FaqI GGGAC 3 cut(s) 941, 1007, 1856
FbaI TGATCA 1 cut(s) 448
FblI GTMKAC 3 cut(s) 429, 566, 1314
FokI GGATG 3 cut(s) 1210, 1310, 1469
FriOI GRGCYC 1 cut(s) 1028
FspBI CTAG 9 cut(s) 96, 575, 770, 959, 1160, 1197, 1286, 1643, 1958
GlaI GCGC 1 cut(s) 495
GsuI CTGGAG 3 cut(s) 1305, 1514, 1896
HapII CCGG 2 cut(s) 213, 2183
HgaI GACGC 1 cut(s) 1955
HhaI GCGC 1 cut(s) 496
Hin1I GRCGYC 3 cut(s) 1392, 1973, 2003
Hin6I GCGC 1 cut(s) 494
HinP1I GCGC 1 cut(s) 494
HindIII AAGCTT 2 cut(s) 897, 2048
HinfI GANTC 5 cut(s) 811, 944, 1404, 1811, 2236
HpaII CCGG 2 cut(s) 213, 2183
Hpy166II GTNNAC 5 cut(s) 136, 430, 567, 1315, 1729
Hpy188I TCNGA 8 cut(s) 103, 223, 257, 552, 810, 1135, 1234, 2046
Hpy188III TCNNGA 6 cut(s) 440, 643, 670, 799, 1021, 1999
Hpy8I GTNNAC 5 cut(s) 136, 430, 567, 1315, 1729
Hpy99I CGWCG 6 cut(s) 1446, 1758, 1825, 1850, 1889, 2024
HpyCH4III ACNGT 8 cut(s) 133, 265, 1187, 1746, 1825, 1846, 1870, 2010
HpyCH4IV ACGT 5 cut(s) 1392, 1444, 1973, 2003, 2220
HpyF10VI GCNNNNNNNGC 6 cut(s) 297, 524, 1200, 1764, 1847, 1856
HpyF3I CTNAG 2 cut(s) 807, 1927
HpySE526I ACGT 5 cut(s) 1392, 1444, 1973, 2003, 2220
Hsp92I GRCGYC 3 cut(s) 1392, 1973, 2003
HspAI GCGC 1 cut(s) 494
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 7 cut(s) 448, 766, 875, 918, 1066, 1995, 2016
LmnI GCTCC 4 cut(s) 697, 1060, 1225, 1266
LweI GCATC 4 cut(s) 792, 1042, 1584, 2109
MaeI CTAG 9 cut(s) 96, 575, 770, 959, 1160, 1197, 1286, 1643, 1958
MaeII ACGT 5 cut(s) 1392, 1444, 1973, 2003, 2220
MaeIII GTNAC 8 cut(s) 56, 966, 1033, 1124, 1974, 2004, 2089, 2185
MalI GATC 7 cut(s) 450, 768, 877, 920, 1068, 1997, 2018
MboI GATC 7 cut(s) 448, 766, 875, 918, 1066, 1995, 2016
MflI RGATCY 3 cut(s) 766, 918, 1066
MhlI GDGCHC 5 cut(s) 138, 694, 908, 1028, 1820
MlsI TGGCCA 2 cut(s) 393, 1841
MluCI AATT 9 cut(s) 162, 409, 888, 972, 1085, 1290, 1651, 2127, 2317
MluNI TGGCCA 2 cut(s) 393, 1841
MlyI GAGTC 4 cut(s) 820, 1413, 1820, 2230
MmeI TCCRAC 4 cut(s) 152, 1274, 1462, 1527
Mox20I TGGCCA 2 cut(s) 393, 1841
MscI TGGCCA 2 cut(s) 393, 1841
MseI TTAA 8 cut(s) 165, 317, 579, 870, 1398, 1410, 1650, 2320
MslI CAYNNNNRTG 4 cut(s) 702, 1004, 1412, 2117
Msp20I TGGCCA 2 cut(s) 393, 1841
MspA1I CMGCKG 2 cut(s) 616, 1040
MspI CCGG 2 cut(s) 213, 2183
MspR9I CCNGG 6 cut(s) 214, 330, 466, 1518, 1708, 1875
Mva1269I GAATGC 2 cut(s) 253, 1696
MvaI CCWGG 5 cut(s) 330, 466, 1518, 1708, 1875
MvnI CGCG 2 cut(s) 496, 1022
MwoI GCNNNNNNNGC 6 cut(s) 297, 524, 1200, 1764, 1847, 1856
NciI CCSGG 1 cut(s) 214
NcoI CCATGG 1 cut(s) 922
NdeII GATC 7 cut(s) 448, 766, 875, 918, 1066, 1995, 2016
NlaIV GGNNCC 4 cut(s) 327, 652, 920, 1068
NmeAIII GCCGAG 3 cut(s) 553, 1834, 2037
NmuCI GTSAC 7 cut(s) 56, 1033, 1124, 1974, 2004, 2089, 2185
NruI TCGCGA 1 cut(s) 1022
NspI RCATGY 3 cut(s) 406, 527, 1367
NspV TTCGAA 1 cut(s) 347
PasI CCCWGGG 1 cut(s) 465
PceI AGGCCT 1 cut(s) 609
PciI ACATGT 1 cut(s) 402
PcsI WCGNNNNNNNCGW 2 cut(s) 2000, 2019
PctI GAATGC 2 cut(s) 253, 1696
PfeI GAWTC 1 cut(s) 944
Pfl23II CGTACG 1 cut(s) 2010
PleI GAGTC 4 cut(s) 819, 1412, 1819, 2230
PpsI GAGTC 4 cut(s) 819, 1412, 1819, 2230
Ppu21I YACGTR 1 cut(s) 2221
PscI ACATGT 1 cut(s) 402
PshAI GACNNNNGTC 1 cut(s) 435
Psp124BI GAGCTC 1 cut(s) 1028
Psp6I CCWGG 5 cut(s) 328, 464, 1516, 1706, 1873
PspEI GGTNACC 2 cut(s) 1124, 2089
PspGI CCWGG 5 cut(s) 328, 464, 1516, 1706, 1873
PspLI CGTACG 1 cut(s) 2010
PspN4I GGNNCC 4 cut(s) 327, 652, 920, 1068
PspPI GGNCC 6 cut(s) 662, 1193, 1205, 1520, 1783, 1803
PstI CTGCAG 2 cut(s) 283, 1169
PsuI RGATCY 3 cut(s) 766, 918, 1066
PvuII CAGCTG 1 cut(s) 616
RruI TCGCGA 1 cut(s) 1022
RsaI GTAC 6 cut(s) 1180, 1748, 2012, 2219, 2246, 2253
RsaNI GTAC 6 cut(s) 1179, 1747, 2011, 2218, 2245, 2252
RseI CAYNNNNRTG 4 cut(s) 702, 1004, 1412, 2117
Rsr2I CGGWCCG 1 cut(s) 1803
RsrII CGGWCCG 1 cut(s) 1803
SacI GAGCTC 1 cut(s) 1028
SaqAI TTAA 8 cut(s) 165, 317, 579, 870, 1398, 1410, 1650, 2320
Sau3AI GATC 7 cut(s) 448, 766, 875, 918, 1066, 1995, 2016
Sau96I GGNCC 6 cut(s) 662, 1193, 1205, 1520, 1783, 1803
ScaI AGTACT 1 cut(s) 1748
SchI GAGTC 4 cut(s) 820, 1413, 1820, 2230
ScrFI CCNGG 6 cut(s) 214, 330, 466, 1518, 1708, 1875
SduI GDGCHC 5 cut(s) 138, 694, 908, 1028, 1820
SfaNI GCATC 4 cut(s) 792, 1042, 1584, 2109
SfcI CTRYAG 2 cut(s) 279, 1165
SfuI TTCGAA 1 cut(s) 347
SinI GGWCC 3 cut(s) 662, 1520, 1803
SmiMI CAYNNNNRTG 4 cut(s) 702, 1004, 1412, 2117
SmlI CTYRAG 1 cut(s) 1775
SmoI CTYRAG 1 cut(s) 1775
SpeI ACTAGT 2 cut(s) 574, 1642
Sse9I AATT 9 cut(s) 162, 409, 888, 972, 1085, 1290, 1651, 2127, 2317
SseBI AGGCCT 1 cut(s) 609
SspI AATATT 1 cut(s) 80
SspMI CTAG 9 cut(s) 96, 575, 770, 959, 1160, 1197, 1286, 1643, 1958
SstI GAGCTC 1 cut(s) 1028
StuI AGGCCT 1 cut(s) 609
StyD4I CCNGG 6 cut(s) 212, 328, 464, 1516, 1706, 1873
StyI CCWWGG 1 cut(s) 922
TaaI ACNGT 8 cut(s) 133, 265, 1187, 1746, 1825, 1846, 1870, 2010
TaiI ACGT 5 cut(s) 1395, 1447, 1976, 2006, 2223
TaqI TCGA 7 cut(s) 347, 814, 1441, 1820, 1994, 2019, 2132
TaqII GACCGA 1 cut(s) 413
TasI AATT 9 cut(s) 162, 409, 888, 972, 1085, 1290, 1651, 2127, 2317
TatI WGTACW 3 cut(s) 1746, 2244, 2251
TauI GCSGC 5 cut(s) 1206, 1770, 1859, 1862, 1899
TfiI GAWTC 1 cut(s) 944
Tru1I TTAA 8 cut(s) 165, 317, 579, 870, 1398, 1410, 1650, 2320
Tru9I TTAA 8 cut(s) 165, 317, 579, 870, 1398, 1410, 1650, 2320
TscAI CASTG 3 cut(s) 138, 1174, 2121
TseFI GTSAC 7 cut(s) 56, 1033, 1124, 1974, 2004, 2089, 2185
Tsp45I GTSAC 7 cut(s) 56, 1033, 1124, 1974, 2004, 2089, 2185
TspGWI ACGGA 3 cut(s) 1293, 2265, 2281
TspRI CASTG 3 cut(s) 138, 1174, 2121
VneI GTGCAC 1 cut(s) 134
VpaK11BI GGWCC 3 cut(s) 662, 1520, 1803
XagI CCTNNNNNAGG 2 cut(s) 305, 1775
XapI RAATTY 2 cut(s) 888, 1085
XceI RCATGY 3 cut(s) 406, 527, 1367
XcmI CCANNNNNNNNNTGG 1 cut(s) 1432
XmiI GTMKAC 3 cut(s) 429, 566, 1314
XspI CTAG 9 cut(s) 96, 575, 770, 959, 1160, 1197, 1286, 1643, 1958
ZraI GACGTC 3 cut(s) 1393, 1974, 2004
ZrmI AGTACT 1 cut(s) 1748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.