Rh5BG018600

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000724
Physical Location & Seq
Forward (+)
1402818 .. 1407275
4458 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG018600.1

Sequence Viewer

Length: 2106 bp
ATGGAAGAAATACGTACTTCCGAACAATCCAAAGCCCTCTATCAAAGCCTCAGAGACGGTGACGTATTAGAAGTGGTGAAAGCTTATGCAAAAGCTACTACTCTTGATGCAACATCGATATCAAAGCTAATTCCAACAGTGCACGACGACACAATCCTCCATATGGCGATTTACATGAAGCAAGAAAGCATCGCAATAGGAATCATCGAAATAAGCAGGCATAATGACGAGCTAATTAAAAAACCCAATGCGTTTGGAAACACAGTTCTTCATGAGGCTGCAGCAACTGATTGTGTACGTGTTGTGGAGAAGTTGTTGGAAAAGGCACCGGATTTGCTGTCCATTCCAAACAATGAAGGGGAAATGCCTCTCTATACAGCAGCTCACTTTGGCCAGAAAGAAATTTTCATTTTGCTGGCTGAAAAACTAAAAAACAATGATTATGATCTAATTCGTCCCCATTTGTGCTCCCTAAAAGGTTCCTCCATATGCAGTACCGTTCTTCATGCTGCAATACATGCCGAGTTCTTTGAGCTTGCACTTTGGATAGCTAGAAAGCCGGAGTATGCGAAGATTGTTCAACTAAAAGACAAAAATTTGAAGACGCCTCTTCAGCTCCTCTCATCCAATTCTTCAGCATTCAAGAGTGGAATGAAATGTGGACTGATCAAGAGGTTCATTTACAACTGTGCTCCATGTGAGAATGCTAAAAAAGAAGATGCTCATGAACAAAATTCCAGTGATAACAGTCATGTTCAAAGTTCCAGTAGATCAGCTACTCTGTTTCTAAGGCGAGTCCTCTCTTCAGATACGTATACAAATTTTCTTTCGGCAGTTAATAGATCAGTTTGGATTGTTCTTCGAGCATGGCGAATGATGAGGAAGATTTATGACGAAAAGAACAAGCATGGACTGGCTTTAGAACTAGCCCAGTTACTAATTGCAGAAGACTATCAATCGAATCCAAAAACAAAAGCGCTATCCCAGTCAGAGACTCCAGATATCCCATTGTTCATTGCAACAAGCAATGGAATATTAGAAATTGTGGAGGAGACACTTAGAGTCTACCCTTTGTCACTTGAGTATACAAACAAAGAGAAGCAGAACATACTGCACCTCGCTATAATGCACCGTCACTCGCACATCTTTGATCGAGTGACGAAGAGGAACAAGAGGTTAATGCCAAGGTTGGCTCGACAAACCGATAAGGATGGATTCACAATACTGCACCATGCTGGAAACATGGAATTTTATAAAGGAGGCACCCAGCCTGGTCCGGCACTTCAGTTGCAAGAAGAGTTAGAATGGTATGAGAAGGTGGAGGAGTTTGTCCCACGATATTATGCAATGAACCGCAGCAGCAGAAATAATGAACAAAAAGAGACACCTGAAGAGTTCTATAGTCGAAAATTTAAGGAAACTGTAGAGGAATTATCTCAGAACGTGGATAAGGTAATACCACCCAAATCTGTAAATGACCTTGGCTGGGACGAAAGCAAAGAGGAGACCCCCAGAGAGTACTTTGGTCGAACCCATAGTGAGCTCCTCGAGGAAGCACAAGAATGGCTAAAGCGGACCTCCGACTCGTGCTCCATGGTGGCCGTACTAATTGCCTCCGTCGCCTTCGCGGCCGCTTTCACCATCCCGGGCGGCAACAACGGCAGAAACGGCCAGCCCATTCTCATCGACAGCCCTTTCTTCTTGGTCTTCATCATCACTAACGCTCTCTCCATCGCCAGCTCGCTGACGTCACTCGCCATGTTCCTCTCCATCCTGTCGTCACCGTACGAGCTCGACGACTTCCACCGCTCTCTCCCTCGGAAGCTCATCCTCGGCTTCACCTCCCTCTTCTTCTCGGTGGCCGTCACCATGCTCGCCTTTGCCTCCACAATGATGCTGACAATTCCGATGAAGAAGCGCCTGACTACAACTCTTATATACTGCGTTGCGGTTCTTCCGGTCACCATGTTTGCGCTGTTGCAGTACCCGTTGTGGGAGGCCTTCAAAAGGACTGGAAAATCCTCAGTCAAAGTCGTGAAAAGAATTCTTCCATGGCCACTTACCGAAACTGCTTGCTCAAAGGCCACTAAGGCCCACACTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

701

Amino Acids

79.38

Weight (kDa)

8.34

Isoelectric Point (pI)

46.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 53 - 142 4.4e-07 Ankyrin repeats (3 copies)
Ank_4 PF13637 90 - 139 1e-05 Ankyrin repeats (many copies)
PGG PF13962 521 - 633 7e-26 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1254
AatII GACGTC 1 cut(s) 1751
AbsI CCTCGAGG 1 cut(s) 1547
AccB1I GGYRCC 2 cut(s) 325, 1262
AccBSI CCGCTC 1 cut(s) 1809
AccI GTMKAC 3 cut(s) 815, 1065, 1085
AccII CGCG 1 cut(s) 1628
AciI CCGC 7 cut(s) 1354, 1573, 1628, 1632, 1650, 1807, 1949
AcoI YGGCCR 6 cut(s) 391, 1599, 1629, 1669, 1860, 2054
AcsI RAATTY 7 cut(s) 402, 595, 733, 820, 1247, 1409, 2043
AcuI CTGAAG 5 cut(s) 596, 618, 789, 1268, 1410
AcyI GRCGYC 2 cut(s) 605, 1748
AdeI CACNNNGTG 1 cut(s) 2103
AfaI GTAC 7 cut(s) 16, 297, 496, 1520, 1605, 1787, 1985
AfeI AGCGCT 1 cut(s) 978
AfiI CCNNNNNNNGG 4 cut(s) 163, 1486, 1993, 2007
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 5 cut(s) 581, 601, 643, 758, 2005
AjnI CCWGG 1 cut(s) 1270
AloI GAACNNNNNNTCC 2 cut(s) 249, 281
Alw21I GWGCWC 6 cut(s) 144, 470, 694, 1545, 1592, 1794
Alw26I GTCTC 5 cut(s) 48, 986, 1046, 1376, 1499
Alw44I GTGCAC 1 cut(s) 140
AlwNI CAGNNNCTG 1 cut(s) 287
Ama87I CYCGRG 2 cut(s) 1547, 1645
Aor51HI AGCGCT 1 cut(s) 978
AoxI GGCC 9 cut(s) 391, 1599, 1629, 1669, 1860, 1998, 2054, 2082, 2091
ApaLI GTGCAC 1 cut(s) 140
ApeKI GCWGC 6 cut(s) 278, 281, 380, 509, 1356, 1359
ApoI RAATTY 7 cut(s) 402, 595, 733, 820, 1247, 1409, 2043
AspLEI GCGC 3 cut(s) 979, 1920, 1975
AspS9I GGNCC 3 cut(s) 1274, 1575, 2092
AsuC2I CCSGG 2 cut(s) 1646, 1647
AsuHPI GGTGA 7 cut(s) 71, 88, 1630, 1773, 1831, 1858, 1954
AvaI CYCGRG 2 cut(s) 1547, 1645
AvaII GGWCC 2 cut(s) 1274, 1575
BaeGI GKGCMC 1 cut(s) 144
BalI TGGCCA 2 cut(s) 393, 2056
BanI GGYRCC 2 cut(s) 325, 1262
BanII GRGCYC 2 cut(s) 1545, 1794
BauI CACGAG 1 cut(s) 1585
BbsI GAAGAC 3 cut(s) 608, 954, 1699
Bbv12I GWGCWC 6 cut(s) 144, 470, 694, 1545, 1592, 1794
BbvI GCAGC 6 cut(s) 265, 293, 392, 496, 1368, 1371
BccI CCATC 4 cut(s) 1205, 1649, 1739, 1778
BceAI ACGGC 4 cut(s) 1586, 1675, 1684, 1847
BciT130I CCWGG 1 cut(s) 1272
BclI TGATCA 1 cut(s) 666
BcnI CCSGG 2 cut(s) 1646, 1647
BcoDI GTCTC 5 cut(s) 48, 986, 1046, 1376, 1499
BfaI CTAG 2 cut(s) 552, 926
BfmI CTRYAG 3 cut(s) 279, 1399, 1422
BfoI RGCGCY 2 cut(s) 980, 1921
BglI GCCNNNNNGGC 2 cut(s) 1628, 2090
BisI GCNGC 9 cut(s) 279, 282, 381, 510, 1357, 1360, 1629, 1632, 1651
BlsI GCNGC 9 cut(s) 280, 283, 382, 511, 1358, 1361, 1630, 1633, 1652
BmcAI AGTACT 1 cut(s) 1520
Bme1390I CCNGG 3 cut(s) 1272, 1646, 1647
Bme18I GGWCC 2 cut(s) 1274, 1575
BmeT110I CYCGRG 2 cut(s) 1547, 1645
BmgT120I GGNCC 3 cut(s) 1274, 1575, 2092
BmiI GGNNCC 3 cut(s) 327, 481, 1264
BmrFI CCNGG 3 cut(s) 1272, 1646, 1647
BmrI ACTGGG 2 cut(s) 925, 979
BmsI GCATC 4 cut(s) 97, 198, 709, 1884
BmuI ACTGGG 2 cut(s) 925, 979
BpiI GAAGAC 3 cut(s) 608, 954, 1699
BpmI CTGGAG 1 cut(s) 981
BpuEI CTTGAG 1 cut(s) 1100
BpuMI CCSGG 2 cut(s) 1646, 1647
Bsa29I ATCGAT 1 cut(s) 116
BsaAI YACGTR 3 cut(s) 14, 299, 813
BsaBI GATNNNNATC 1 cut(s) 444
BsaHI GRCGYC 2 cut(s) 605, 1748
BsaI GGTCTC 1 cut(s) 1499
BsaJI CCNNGG 7 cut(s) 1184, 1480, 1593, 1645, 1817, 1831, 2051
BsaWI WCCGGW 2 cut(s) 328, 1957
BsaXI ACNNNNNCTCC 6 cut(s) 299, 329, 1574, 1604, 1712, 1742
Bsc4I CCNNNNNNNGG 4 cut(s) 163, 1486, 1993, 2007
Bse1I ACTGG 6 cut(s) 738, 765, 918, 931, 985, 2017
Bse3DI GCAATG 3 cut(s) 1014, 1033, 1353
Bse8I GATNNNNATC 1 cut(s) 444
BseBI CCWGG 1 cut(s) 1272
BseCI ATCGAT 1 cut(s) 116
BseDI CCNNGG 7 cut(s) 1184, 1480, 1593, 1645, 1817, 1831, 2051
BseGI GGATG 5 cut(s) 623, 1216, 1641, 1770, 1827
BseJI GATNNNNATC 1 cut(s) 444
BseLI CCNNNNNNNGG 4 cut(s) 163, 1486, 1993, 2007
BseMI GCAATG 3 cut(s) 1014, 1033, 1353
BseMII CTCAG 3 cut(s) 64, 1451, 2037
BseNI ACTGG 6 cut(s) 738, 765, 918, 931, 985, 2017
BseRI GAGGAG 5 cut(s) 608, 1064, 1337, 1517, 1535
BseSI GKGCMC 1 cut(s) 144
BseX3I CGGCCG 1 cut(s) 1629
BseXI GCAGC 6 cut(s) 265, 293, 392, 496, 1368, 1371
BseYI CCCAGC 2 cut(s) 1266, 1485
BsgI GTGCAG 2 cut(s) 1097, 1211
Bsh1236I CGCG 1 cut(s) 1628
Bsh1285I CGRYCG 1 cut(s) 1632
BshFI GGCC 9 cut(s) 393, 1601, 1631, 1671, 1862, 2000, 2056, 2084, 2093
BshNI GGYRCC 2 cut(s) 325, 1262
BshVI ATCGAT 1 cut(s) 116
BsiEI CGRYCG 1 cut(s) 1632
BsiHKAI GWGCWC 6 cut(s) 144, 470, 694, 1545, 1592, 1794
BsiHKCI CYCGRG 2 cut(s) 1547, 1645
BsiSI CCGG 5 cut(s) 329, 560, 1277, 1646, 1958
BsiWI CGTACG 1 cut(s) 1785
BslFI GGGAC 3 cut(s) 441, 1316, 1502
BslI CCNNNNNNNGG 4 cut(s) 163, 1486, 1993, 2007
BsmAI GTCTC 5 cut(s) 48, 986, 1046, 1376, 1499
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 3 cut(s) 441, 1316, 1502
BsmI GAATGC 2 cut(s) 638, 709
BsnI GGCC 9 cut(s) 393, 1601, 1631, 1671, 1862, 2000, 2056, 2084, 2093
Bso31I GGTCTC 1 cut(s) 1499
BsoBI CYCGRG 2 cut(s) 1547, 1645
Bsp1286I GDGCHC 6 cut(s) 144, 470, 694, 1545, 1592, 1794
Bsp143I GATC 5 cut(s) 445, 666, 770, 842, 1150
Bsp19I CCATGG 2 cut(s) 1593, 2051
BspACI CCGC 7 cut(s) 1354, 1573, 1628, 1632, 1650, 1807, 1949
BspANI GGCC 9 cut(s) 393, 1601, 1631, 1671, 1862, 2000, 2056, 2084, 2093
BspCNI CTCAG 3 cut(s) 63, 1450, 2036
BspDI ATCGAT 1 cut(s) 116
BspFNI CGCG 1 cut(s) 1628
BspHI TCATGA 2 cut(s) 271, 724
BspLI GGNNCC 3 cut(s) 327, 481, 1264
BspMAI CTGCAG 1 cut(s) 283
BspT107I GGYRCC 2 cut(s) 325, 1262
BspTNI GGTCTC 1 cut(s) 1499
BsrBI CCGCTC 1 cut(s) 1809
BsrDI GCAATG 3 cut(s) 1014, 1033, 1353
BsrI ACTGG 6 cut(s) 738, 765, 918, 931, 985, 2017
BssECI CCNNGG 7 cut(s) 1184, 1480, 1593, 1645, 1817, 1831, 2051
BssMI GATC 5 cut(s) 445, 666, 770, 842, 1150
BssNAI GTATAC 2 cut(s) 816, 1086
BssNI GRCGYC 2 cut(s) 605, 1748
BssSI CACGAG 1 cut(s) 1585
BssT1I CCWWGG 4 cut(s) 1184, 1480, 1593, 2051
Bst1107I GTATAC 2 cut(s) 816, 1086
Bst2BI CACGAG 1 cut(s) 1585
Bst2UI CCWGG 1 cut(s) 1272
Bst4CI ACNGT 9 cut(s) 59, 139, 265, 499, 689, 749, 1133, 1423, 1785
Bst6I CTCTTC 6 cut(s) 615, 808, 1157, 1290, 1386, 1853
BstACI GRCGYC 2 cut(s) 605, 1748
BstAPI GCANNNNNTGC 1 cut(s) 518
BstBAI YACGTR 3 cut(s) 14, 299, 813
BstC8I GCNNGC 8 cut(s) 218, 417, 537, 1673, 1738, 1742, 1875, 2074
BstDEI CTNAG 6 cut(s) 50, 788, 1058, 1437, 2023, 2088
BstDSI CCRYGG 2 cut(s) 1593, 2051
BstEII GGTNACC 1 cut(s) 1960
BstENI CCTNNNNNAGG 1 cut(s) 2005
BstF5I GGATG 5 cut(s) 623, 1216, 1641, 1770, 1827
BstFNI CGCG 1 cut(s) 1628
BstH2I RGCGCY 2 cut(s) 980, 1921
BstHHI GCGC 3 cut(s) 979, 1920, 1975
BstKTI GATC 5 cut(s) 448, 669, 773, 845, 1153
BstMAI GTCTC 5 cut(s) 48, 986, 1046, 1376, 1499
BstMBI GATC 5 cut(s) 445, 666, 770, 842, 1150
BstMCI CGRYCG 1 cut(s) 1632
BstMWI GCNNNNNNNGC 7 cut(s) 518, 613, 1619, 1628, 1659, 1668, 2090
BstNI CCWGG 1 cut(s) 1272
BstNSI RCATGY 1 cut(s) 521
BstPI GGTNACC 1 cut(s) 1960
BstSCI CCNGG 3 cut(s) 1270, 1644, 1645
BstSFI CTRYAG 3 cut(s) 279, 1399, 1422
BstSLI GKGCMC 1 cut(s) 144
BstSNI TACGTA 2 cut(s) 14, 813
BstUI CGCG 1 cut(s) 1628
BstV1I GCAGC 6 cut(s) 265, 293, 392, 496, 1368, 1371
BstV2I GAAGAC 3 cut(s) 608, 954, 1699
BstZ17I GTATAC 2 cut(s) 816, 1086
BstZI CGGCCG 1 cut(s) 1629
Bsu15I ATCGAT 1 cut(s) 116
BsuRI GGCC 9 cut(s) 393, 1601, 1631, 1671, 1862, 2000, 2056, 2084, 2093
BsuTUI ATCGAT 1 cut(s) 116
BtgI CCRYGG 2 cut(s) 1593, 2051
BtgZI GCGATG 2 cut(s) 175, 1717
BtsCI GGATG 5 cut(s) 623, 1216, 1641, 1770, 1827
BtsIMutI CAGTG 2 cut(s) 144, 745
Cac8I GCNNGC 8 cut(s) 218, 417, 537, 1673, 1738, 1742, 1875, 2074
CaiI CAGNNNCTG 1 cut(s) 287
CciI TCATGA 2 cut(s) 271, 724
CciNI GCGGCCGC 1 cut(s) 1629
CfoI GCGC 3 cut(s) 979, 1920, 1975
Cfr13I GGNCC 3 cut(s) 1274, 1575, 2092
Cfr9I CCCGGG 1 cut(s) 1645
ClaI ATCGAT 1 cut(s) 116
CseI GACGC 1 cut(s) 613
Csp6I GTAC 7 cut(s) 15, 296, 495, 1519, 1604, 1786, 1984
CviQI GTAC 7 cut(s) 15, 296, 495, 1519, 1604, 1786, 1984
DdeI CTNAG 6 cut(s) 50, 788, 1058, 1437, 2023, 2088
DpnI GATC 5 cut(s) 447, 668, 772, 844, 1152
DpnII GATC 5 cut(s) 445, 666, 770, 842, 1150
DraIII CACNNNGTG 1 cut(s) 2103
EaeI YGGCCR 6 cut(s) 391, 1599, 1629, 1669, 1860, 2054
EagI CGGCCG 1 cut(s) 1629
Eam1104I CTCTTC 6 cut(s) 615, 808, 1157, 1290, 1386, 1853
EarI CTCTTC 6 cut(s) 615, 808, 1157, 1290, 1386, 1853
Ecl136II GAGCTC 2 cut(s) 1543, 1792
EclXI CGGCCG 1 cut(s) 1629
Eco105I TACGTA 2 cut(s) 14, 813
Eco130I CCWWGG 4 cut(s) 1184, 1480, 1593, 2051
Eco147I AGGCCT 1 cut(s) 2000
Eco24I GRGCYC 2 cut(s) 1545, 1794
Eco31I GGTCTC 1 cut(s) 1499
Eco32I GATATC 2 cut(s) 120, 1003
Eco47I GGWCC 2 cut(s) 1274, 1575
Eco47III AGCGCT 1 cut(s) 978
Eco52I CGGCCG 1 cut(s) 1629
Eco53kI GAGCTC 2 cut(s) 1543, 1792
Eco57I CTGAAG 5 cut(s) 596, 618, 789, 1268, 1410
Eco88I CYCGRG 2 cut(s) 1547, 1645
Eco91I GGTNACC 1 cut(s) 1960
EcoICRI GAGCTC 2 cut(s) 1543, 1792
EcoNI CCTNNNNNAGG 1 cut(s) 2005
EcoO65I GGTNACC 1 cut(s) 1960
EcoRI GAATTC 1 cut(s) 2043
EcoRII CCWGG 1 cut(s) 1270
EcoRV GATATC 2 cut(s) 120, 1003
EcoT14I CCWWGG 4 cut(s) 1184, 1480, 1593, 2051
EcoT38I GRGCYC 2 cut(s) 1545, 1794
ErhI CCWWGG 4 cut(s) 1184, 1480, 1593, 2051
Esp3I CGTCTC 1 cut(s) 48
FaqI GGGAC 3 cut(s) 441, 1316, 1502
FauNDI CATATG 2 cut(s) 162, 488
FbaI TGATCA 1 cut(s) 666
FblI GTMKAC 3 cut(s) 815, 1065, 1085
Fnu4HI GCNGC 9 cut(s) 279, 282, 381, 510, 1357, 1360, 1629, 1632, 1651
FokI GGATG 5 cut(s) 610, 1223, 1628, 1757, 1814
FriOI GRGCYC 2 cut(s) 1545, 1794
Fsp4HI GCNGC 9 cut(s) 279, 282, 381, 510, 1357, 1360, 1629, 1632, 1651
FspBI CTAG 2 cut(s) 552, 926
GlaI GCGC 3 cut(s) 978, 1919, 1974
GluI GCNGC 9 cut(s) 279, 282, 381, 510, 1357, 1360, 1629, 1632, 1651
GsaI CCCAGC 2 cut(s) 1270, 1489
GsuI CTGGAG 1 cut(s) 981
HaeII RGCGCY 2 cut(s) 980, 1921
HaeIII GGCC 9 cut(s) 393, 1601, 1631, 1671, 1862, 2000, 2056, 2084, 2093
HapII CCGG 5 cut(s) 329, 560, 1277, 1646, 1958
HgaI GACGC 1 cut(s) 613
HhaI GCGC 3 cut(s) 979, 1920, 1975
Hin1I GRCGYC 2 cut(s) 605, 1748
Hin6I GCGC 3 cut(s) 977, 1918, 1973
HinP1I GCGC 3 cut(s) 977, 1918, 1973
HindIII AAGCTT 1 cut(s) 81
HinfI GANTC 7 cut(s) 201, 795, 961, 994, 1062, 1215, 1583
HpaII CCGG 5 cut(s) 329, 560, 1277, 1646, 1958
HphI GGTGA 7 cut(s) 71, 88, 1630, 1773, 1831, 1858, 1954
Hpy166II GTNNAC 6 cut(s) 142, 296, 662, 816, 1066, 1086
Hpy188I TCNGA 8 cut(s) 22, 53, 808, 991, 1440, 1582, 1821, 1908
Hpy188III TCNNGA 8 cut(s) 104, 272, 643, 670, 725, 998, 2035, 2103
Hpy8I GTNNAC 6 cut(s) 142, 296, 662, 816, 1066, 1086
Hpy99I CGWCG 3 cut(s) 149, 1622, 1799
HpyAV CCTTC 4 cut(s) 350, 1309, 1633, 2011
HpyCH4III ACNGT 9 cut(s) 59, 139, 265, 499, 689, 749, 1133, 1423, 1785
HpyCH4IV ACGT 6 cut(s) 13, 63, 298, 812, 1443, 1748
HpyF10VI GCNNNNNNNGC 7 cut(s) 518, 613, 1619, 1628, 1659, 1668, 2090
HpyF3I CTNAG 6 cut(s) 50, 788, 1058, 1437, 2023, 2088
HpySE526I ACGT 6 cut(s) 13, 63, 298, 812, 1443, 1748
Hsp92I GRCGYC 2 cut(s) 605, 1748
HspAI GCGC 3 cut(s) 977, 1918, 1973
Ksp22I TGATCA 1 cut(s) 666
Kzo9I GATC 5 cut(s) 445, 666, 770, 842, 1150
LmnI GCTCC 5 cut(s) 473, 621, 697, 1548, 1595
Lsp1109I GCAGC 6 cut(s) 265, 293, 392, 496, 1368, 1371
LweI GCATC 4 cut(s) 97, 198, 709, 1884
MaeI CTAG 2 cut(s) 552, 926
MaeII ACGT 6 cut(s) 13, 63, 298, 812, 1443, 1748
MaeIII GTNAC 9 cut(s) 59, 933, 1074, 1133, 1156, 1749, 1779, 1864, 1960
MalI GATC 5 cut(s) 447, 668, 772, 844, 1152
MbiI CCGCTC 1 cut(s) 1809
MboI GATC 5 cut(s) 445, 666, 770, 842, 1150
MhlI GDGCHC 6 cut(s) 144, 470, 694, 1545, 1592, 1794
MlsI TGGCCA 2 cut(s) 393, 2056
MluNI TGGCCA 2 cut(s) 393, 2056
MlyI GAGTC 4 cut(s) 804, 988, 1071, 1577
MmeI TCCRAC 3 cut(s) 158, 297, 1605
Mox20I TGGCCA 2 cut(s) 393, 2056
MscI TGGCCA 2 cut(s) 393, 2056
MseI TTAA 4 cut(s) 237, 837, 1178, 1413
MslI CAYNNNNRTG 2 cut(s) 1561, 1892
Msp20I TGGCCA 2 cut(s) 393, 2056
MspI CCGG 5 cut(s) 329, 560, 1277, 1646, 1958
MspR9I CCNGG 3 cut(s) 1272, 1646, 1647
Mva1269I GAATGC 2 cut(s) 638, 709
MvaI CCWGG 1 cut(s) 1272
MvnI CGCG 1 cut(s) 1628
MwoI GCNNNNNNNGC 7 cut(s) 518, 613, 1619, 1628, 1659, 1668, 2090
NciI CCSGG 2 cut(s) 1646, 1647
NcoI CCATGG 2 cut(s) 1593, 2051
NdeI CATATG 2 cut(s) 162, 488
NdeII GATC 5 cut(s) 445, 666, 770, 842, 1150
NlaIV GGNNCC 3 cut(s) 327, 481, 1264
NmeAIII GCCGAG 2 cut(s) 547, 1812
NmuCI GTSAC 8 cut(s) 59, 1074, 1133, 1156, 1749, 1779, 1864, 1960
NotI GCGGCCGC 1 cut(s) 1629
NspI RCATGY 1 cut(s) 521
PaeR7I CTCGAG 1 cut(s) 1547
PagI TCATGA 2 cut(s) 271, 724
PceI AGGCCT 1 cut(s) 2000
PcsI WCGNNNNNNNCGW 2 cut(s) 868, 1794
PctI GAATGC 2 cut(s) 638, 709
PfeI GAWTC 3 cut(s) 201, 961, 1215
Pfl23II CGTACG 1 cut(s) 1785
PkrI GCNGC 9 cut(s) 280, 283, 382, 511, 1358, 1361, 1630, 1633, 1652
PleI GAGTC 4 cut(s) 803, 988, 1070, 1577
PpsI GAGTC 4 cut(s) 803, 988, 1070, 1577
Ppu21I YACGTR 3 cut(s) 14, 299, 813
PsiI TTATAA 1 cut(s) 1254
Psp124BI GAGCTC 2 cut(s) 1545, 1794
Psp6I CCWGG 1 cut(s) 1270
PspEI GGTNACC 1 cut(s) 1960
PspFI CCCAGC 2 cut(s) 1266, 1485
PspGI CCWGG 1 cut(s) 1270
PspLI CGTACG 1 cut(s) 1785
PspN4I GGNNCC 3 cut(s) 327, 481, 1264
PspPI GGNCC 3 cut(s) 1274, 1575, 2092
PspXI VCTCGAGB 1 cut(s) 1547
PstI CTGCAG 1 cut(s) 283
PstNI CAGNNNCTG 1 cut(s) 287
RsaI GTAC 7 cut(s) 16, 297, 496, 1520, 1605, 1787, 1985
RsaNI GTAC 7 cut(s) 15, 296, 495, 1519, 1604, 1786, 1984
RseI CAYNNNNRTG 2 cut(s) 1561, 1892
SacI GAGCTC 2 cut(s) 1545, 1794
SaqAI TTAA 4 cut(s) 237, 837, 1178, 1413
SatI GCNGC 9 cut(s) 279, 282, 381, 510, 1357, 1360, 1629, 1632, 1651
Sau3AI GATC 5 cut(s) 445, 666, 770, 842, 1150
Sau96I GGNCC 3 cut(s) 1274, 1575, 2092
ScaI AGTACT 1 cut(s) 1520
SchI GAGTC 4 cut(s) 804, 988, 1071, 1577
ScrFI CCNGG 3 cut(s) 1272, 1646, 1647
SduI GDGCHC 6 cut(s) 144, 470, 694, 1545, 1592, 1794
SfaNI GCATC 4 cut(s) 97, 198, 709, 1884
SfcI CTRYAG 3 cut(s) 279, 1399, 1422
SfiI GGCCNNNNNGGCC 1 cut(s) 2090
Sfr274I CTCGAG 1 cut(s) 1547
SinI GGWCC 2 cut(s) 1274, 1575
SlaI CTCGAG 1 cut(s) 1547
SmaI CCCGGG 1 cut(s) 1647
SmiMI CAYNNNNRTG 2 cut(s) 1561, 1892
SmlI CTYRAG 2 cut(s) 1079, 1547
SmoI CTYRAG 2 cut(s) 1079, 1547
SnaBI TACGTA 2 cut(s) 14, 813
SseBI AGGCCT 1 cut(s) 2000
SsiI CCGC 7 cut(s) 1354, 1573, 1628, 1632, 1650, 1807, 1949
SspI AATATT 1 cut(s) 1035
SspMI CTAG 2 cut(s) 552, 926
SstI GAGCTC 2 cut(s) 1545, 1794
StuI AGGCCT 1 cut(s) 2000
StyD4I CCNGG 3 cut(s) 1270, 1644, 1645
StyI CCWWGG 4 cut(s) 1184, 1480, 1593, 2051
TaaI ACNGT 9 cut(s) 59, 139, 265, 499, 689, 749, 1133, 1423, 1785
TaiI ACGT 6 cut(s) 16, 66, 301, 815, 1446, 1751
TatI WGTACW 1 cut(s) 1518
TauI GCSGC 3 cut(s) 1631, 1634, 1653
TfiI GAWTC 3 cut(s) 201, 961, 1215
Tru1I TTAA 4 cut(s) 237, 837, 1178, 1413
Tru9I TTAA 4 cut(s) 237, 837, 1178, 1413
TscAI CASTG 2 cut(s) 144, 745
TseFI GTSAC 8 cut(s) 59, 1074, 1133, 1156, 1749, 1779, 1864, 1960
TseI GCWGC 6 cut(s) 278, 281, 380, 509, 1356, 1359
Tsp45I GTSAC 8 cut(s) 59, 1074, 1133, 1156, 1749, 1779, 1864, 1960
TspGWI ACGGA 1 cut(s) 1606
TspMI CCCGGG 1 cut(s) 1645
TspRI CASTG 2 cut(s) 144, 745
VneI GTGCAC 1 cut(s) 140
VpaK11BI GGWCC 2 cut(s) 1274, 1575
XagI CCTNNNNNAGG 1 cut(s) 2005
XapI RAATTY 7 cut(s) 402, 595, 733, 820, 1247, 1409, 2043
XceI RCATGY 1 cut(s) 521
XhoI CTCGAG 1 cut(s) 1547
XmaI CCCGGG 1 cut(s) 1645
XmiI GTMKAC 3 cut(s) 815, 1065, 1085
XspI CTAG 2 cut(s) 552, 926
ZraI GACGTC 1 cut(s) 1749
ZrmI AGTACT 1 cut(s) 1520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.