pycom05g01780

Ankyrin repeat-containing protein At3g12360-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000724
Physical Location & Seq
Forward (+)
2148928 .. 2153152
4225 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g01780.1

Sequence Viewer

Length: 2085 bp
ATGGAAGAGAGAAAGAAAGAGAGGTTGGAGAAAGAGAGAGAAGAAGATGAGAAAACAGAAAGAGCCGGAGAGGCTCTGAGTCGATCAAAAAGGCCATATAAATGGGCCATGCACGACAACTGGGAACCCCTGAAACAATACTATAAGAGAAACTGGGAAGAGGTTTTCATTCCATTGACAACGAACGACGACACTGTACTTCACGTCGTAGCCTATGCCGGGCGGAGCGACGTGCTCGAGTTCTTGATTTCACTTGTGACAACTGCGACCAATGACCCTAGAAAGTTACGGCATGCCTTGGTGCTGGAGAATGGCCACGGGAACACCATCCTCCACGAGGTGGTGGCGTCGGGAAACCTAAAAGCGGCAATGCTGTTGGTAAGCCACGATAACAGCGTTAGGAGTGAGCTAATTGCTAGACAGGATGTTGATCAAACCAATGAGTACGAGCGGGATGTTATTGATGCTAGGTCTAGAAGTATTGTGGAGATTGAGAACAAGCTAGGAGAAACGCCCATCTACAGGGCAGCTTCTTACGGTCACACAAAATTGATCGACTTTTTCCACAAACAATATGTGAATACAGAGCGCCACTTCACCAGATCTGTTGATCGCATGTCCGTTCTTCATATCGCTGTCATTAACCAACATTTTGACACTGCTGTCTGGCTACTGAAAAAGTACCCATTTCTTGCAAAAACAAGGGAAGACGATGGTTTCACAAGCCTTCAATTGCTAGCCCAGATGCCATCTGCCTTCCAAGTCTACAATCGCGAAAGCTTATGGAAGATGCTTATTCATAAATGCCTCTCTGAAGATGATAGTGTTGATGGGACCGCACCAAATCAAAACGGTGATATAGAGAGCAGATGGATTCCACAGCTTCCCACATCTTGCTTGTCGAAGAAGCTCACAAGATGGCCTCGAGTCTTTTCGATCTTGAAGCAAATAAAAAAGGAAAAAACACTATCGGAGCTCACTCGGCTGCTCGTGGAGAAAGACTACTCGTGGGTAGAAAGTAAGAAACGAAAGAAAGACAAGACAATTTCCTTGGTGTCCTCACAGAAAGATATTGCTGCTATTTGTGGTGATGGAAGAGAAACAAAAAAATCTACTATTTATAACTACACCCCACTGCTTATAGCAACTAGCACCGGAATATTACCTCTTGTGAAAAGGATATATGAGGTACATCCTCAGGCGATTGAGGCGCACGATATCGGCAGTCAACAAAACATTTTGCATATGTCAATTAAGCATCGTCAGTTGCAGATATTTAGGCTTGTGAAGAGAAGGAGAGCGATAACTTGGAGGTTAACTTCGAGGATTGACAGTGATGGAAATACCATATTGCATCGAGCTGCAGATATGGCAAATTACTCTCCTGACACTCAACGTGTAAGTGGTCCTGCGTTGCAATTGCAAGAAGAATTACGTTGGATGGTGCGCGTGGGAAAGATAACGCCACCTCATTATATCATGCACCGCAACAACAAGGGTGTGACAGCAGAGGAGCTCTTCAATAGTGAGCATGAAAAGCTACTTCATTCGGCACAAACATGGATAAAAGACACTGCTCAGTCATGCTCGACCGTGGCAGCTCTAGTGGCCACTGTGGTCTTTGCAGCCGCCTACACTGCTCCCGGGGGTTACAATTCAGGCGGTGTTCCTGTTCTCCGACATTCTCCTTTGTTCAGTATCTTTATCATCACAGGCGTTGTCTCGCTCATCAGCTCATTGTCTTCTCTCGTCACGTTTCTCTCGATCTTGACATCTCCATTTGAGTACAAAAATTTCCATCATTCTCTGCCGCTCAGGCTGCATTTAGGATTTGCCTTTCTTTTCTTCTCGCTAGTGACGGCCATGCTTGCGTTCACTGCTACAGTTGTGCTCTTGATTCATCTCAACGAGAAATTGACATGGTCTCTCTTTTACGTTGCTGCGTTTCTTCCTGTGTCTGTATTTGGTATGCAGCTTCCCTTGTATGCGGGATTTTCTCATGCTTTGATATTTCTATTTAAGAAAGTTAAGAAGTTTATAGTAAAACAGAAACCCTCAATCAGTAAGGGTTTTATTGAATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

695

Amino Acids

79.35

Weight (kDa)

9.37

Isoelectric Point (pI)

48.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 163 - 227 5e-06 Ankyrin repeats (3 copies)
PGG PF13962 520 - 630 3.1e-20 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1122
AasI GACNNNNNNGTC 1 cut(s) 662
AccB7I CCANNNNNTGG 1 cut(s) 340
AccBSI CCGCTC 2 cut(s) 451, 1813
AccI GTMKAC 1 cut(s) 765
AccII CGCG 2 cut(s) 774, 1449
AciI CCGC 9 cut(s) 223, 365, 451, 837, 1486, 1629, 1662, 1811, 1988
AcoI YGGCCR 3 cut(s) 313, 1608, 1860
AcsI RAATTY 1 cut(s) 1792
AcuI CTGAAG 1 cut(s) 834
AcyI GRCGYC 1 cut(s) 347
AdeI CACNNNGTG 1 cut(s) 340
AfaI GTAC 5 cut(s) 198, 446, 683, 1191, 1787
AfiI CCNNNNNNNGG 5 cut(s) 219, 337, 340, 364, 522
AflIII ACRYGT 1 cut(s) 1396
AgsI TTSAA 4 cut(s) 731, 943, 1522, 2078
AjiI CACGTC 2 cut(s) 205, 232
AjuI GAANNNNNNNTTGG 2 cut(s) 8, 40
Alw21I GWGCWC 4 cut(s) 237, 978, 1518, 1893
Alw26I GTCTC 2 cut(s) 1726, 1929
Ama87I CYCGRG 3 cut(s) 236, 924, 1643
AoxI GGCC 6 cut(s) 92, 105, 313, 920, 1608, 1860
ApeKI GCWGC 9 cut(s) 527, 985, 1076, 1361, 1598, 1625, 1819, 1940, 1972
ApoI RAATTY 1 cut(s) 1792
AspLEI GCGC 3 cut(s) 591, 1213, 1449
AspS9I GGNCC 3 cut(s) 105, 834, 1406
AsuC2I CCSGG 3 cut(s) 220, 1644, 1645
AsuHPI GGTGA 3 cut(s) 589, 866, 1100
AsuNHI GCTAGC 1 cut(s) 736
AvaI CYCGRG 3 cut(s) 236, 924, 1643
AvaII GGWCC 2 cut(s) 834, 1406
AxyI CCTNAGG 1 cut(s) 1197
BalI TGGCCA 2 cut(s) 315, 1610
BanII GRGCYC 2 cut(s) 978, 1518
BauI CACGAG 3 cut(s) 335, 989, 1006
BbsI GAAGAC 2 cut(s) 714, 1734
Bbv12I GWGCWC 4 cut(s) 237, 978, 1518, 1893
BbvI GCAGC 9 cut(s) 539, 972, 1063, 1348, 1610, 1637, 1806, 1927, 1984
BceAI ACGGC 2 cut(s) 305, 1875
BclI TGATCA 1 cut(s) 430
BcnI CCSGG 3 cut(s) 220, 1644, 1645
BcoDI GTCTC 2 cut(s) 1726, 1929
BfaI CTAG 9 cut(s) 279, 417, 468, 474, 503, 737, 1149, 1604, 1853
BfmI CTRYAG 3 cut(s) 520, 1362, 1881
BfoI RGCGCY 1 cut(s) 592
BglI GCCNNNNNGGC 2 cut(s) 71, 1816
BglII AGATCT 1 cut(s) 602
Bme1390I CCNGG 3 cut(s) 220, 1644, 1645
Bme18I GGWCC 2 cut(s) 834, 1406
BmeT110I CYCGRG 3 cut(s) 236, 924, 1643
BmgBI CACGTC 2 cut(s) 205, 232
BmgT120I GGNCC 3 cut(s) 105, 834, 1406
BmiI GGNNCC 2 cut(s) 126, 835
BmrFI CCNGG 3 cut(s) 220, 1644, 1645
BmrI ACTGGG 2 cut(s) 130, 163
BmsI GCATC 5 cut(s) 454, 735, 780, 1267, 1363
BmtI GCTAGC 1 cut(s) 740
BmuI ACTGGG 2 cut(s) 130, 163
BpiI GAAGAC 2 cut(s) 714, 1734
BpmI CTGGAG 1 cut(s) 326
Bpu10I CCTNAGC 1 cut(s) 1814
BpuMI CCSGG 3 cut(s) 220, 1644, 1645
BsaBI GATNNNNATC 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 347
BsaI GGTCTC 1 cut(s) 1929
BsaJI CCNNGG 6 cut(s) 297, 316, 1050, 1593, 1643, 1644
BsaWI WCCGGW 1 cut(s) 1154
Bsc4I CCNNNNNNNGG 5 cut(s) 219, 337, 340, 364, 522
Bse1I ACTGG 2 cut(s) 125, 158
Bse21I CCTNAGG 1 cut(s) 1197
Bse3DI GCAATG 1 cut(s) 375
Bse8I GATNNNNATC 1 cut(s) 429
BseDI CCNNGG 6 cut(s) 297, 316, 1050, 1593, 1643, 1644
BseGI GGATG 5 cut(s) 327, 430, 460, 1192, 1446
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 5 cut(s) 219, 337, 340, 364, 522
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 4 cut(s) 68, 1211, 1592, 1828
BseNI ACTGG 2 cut(s) 125, 158
BseRI GAGGAG 1 cut(s) 1526
BseXI GCAGC 9 cut(s) 539, 972, 1063, 1348, 1610, 1637, 1806, 1927, 1984
Bsh1236I CGCG 2 cut(s) 774, 1449
Bsh1285I CGRYCG 1 cut(s) 1593
BshFI GGCC 6 cut(s) 94, 107, 315, 922, 1610, 1862
BsiEI CGRYCG 1 cut(s) 1593
BsiHKAI GWGCWC 4 cut(s) 237, 978, 1518, 1893
BsiHKCI CYCGRG 3 cut(s) 236, 924, 1643
BsiSI CCGG 4 cut(s) 66, 219, 1155, 1644
BslFI GGGAC 1 cut(s) 847
BslI CCNNNNNNNGG 5 cut(s) 219, 337, 340, 364, 522
BsmAI GTCTC 2 cut(s) 1726, 1929
BsmFI GGGAC 1 cut(s) 847
BsnI GGCC 6 cut(s) 94, 107, 315, 922, 1610, 1862
Bso31I GGTCTC 1 cut(s) 1929
BsoBI CYCGRG 3 cut(s) 236, 924, 1643
Bsp1286I GDGCHC 4 cut(s) 237, 978, 1518, 1893
Bsp143I GATC 7 cut(s) 83, 430, 552, 602, 610, 936, 1764
Bsp68I TCGCGA 1 cut(s) 774
BspACI CCGC 9 cut(s) 223, 365, 451, 837, 1486, 1629, 1662, 1811, 1988
BspANI GGCC 6 cut(s) 94, 107, 315, 922, 1610, 1862
BspCNI CTCAG 4 cut(s) 69, 1210, 1591, 1827
BspFNI CGCG 2 cut(s) 774, 1449
BspLI GGNNCC 2 cut(s) 126, 835
BspMAI CTGCAG 1 cut(s) 1366
BspOI GCTAGC 1 cut(s) 740
BspQI GCTCTTC 1 cut(s) 1523
BspTNI GGTCTC 1 cut(s) 1929
BsrBI CCGCTC 2 cut(s) 451, 1813
BsrDI GCAATG 1 cut(s) 375
BsrI ACTGG 2 cut(s) 125, 158
BssECI CCNNGG 6 cut(s) 297, 316, 1050, 1593, 1643, 1644
BssMI GATC 7 cut(s) 83, 430, 552, 602, 610, 936, 1764
BssNI GRCGYC 1 cut(s) 347
BssSI CACGAG 3 cut(s) 335, 989, 1006
BssT1I CCWWGG 2 cut(s) 297, 1050
Bst2BI CACGAG 3 cut(s) 335, 989, 1006
Bst4CI ACNGT 7 cut(s) 196, 539, 854, 1334, 1594, 1615, 1885
Bst6I CTCTTC 4 cut(s) 153, 1090, 1283, 1523
BstACI GRCGYC 1 cut(s) 347
BstC8I GCNNGC 3 cut(s) 294, 738, 1869
BstDEI CTNAG 4 cut(s) 77, 1197, 1578, 1814
BstDSI CCRYGG 2 cut(s) 316, 1593
BstENI CCTNNNNNAGG 1 cut(s) 335
BstF5I GGATG 5 cut(s) 327, 430, 460, 1192, 1446
BstFNI CGCG 2 cut(s) 774, 1449
BstH2I RGCGCY 1 cut(s) 592
BstHHI GCGC 3 cut(s) 591, 1213, 1449
BstKTI GATC 7 cut(s) 86, 433, 555, 605, 613, 939, 1767
BstMAI GTCTC 2 cut(s) 1726, 1929
BstMBI GATC 7 cut(s) 83, 430, 552, 602, 610, 936, 1764
BstMCI CGRYCG 1 cut(s) 1593
BstNSI RCATGY 2 cut(s) 296, 619
BstSCI CCNGG 3 cut(s) 218, 1642, 1643
BstSFI CTRYAG 3 cut(s) 520, 1362, 1881
BstUI CGCG 2 cut(s) 774, 1449
BstV1I GCAGC 9 cut(s) 539, 972, 1063, 1348, 1610, 1637, 1806, 1927, 1984
BstV2I GAAGAC 2 cut(s) 714, 1734
BstX2I RGATCY 1 cut(s) 602
BstXI CCANNNNNNTGG 1 cut(s) 102
BstYI RGATCY 1 cut(s) 602
Bsu36I CCTNAGG 1 cut(s) 1197
BsuRI GGCC 6 cut(s) 94, 107, 315, 922, 1610, 1862
BtgI CCRYGG 2 cut(s) 316, 1593
BtrI CACGTC 2 cut(s) 205, 232
BtsCI GGATG 5 cut(s) 327, 430, 460, 1192, 1446
BtsI GCAGTG 5 cut(s) 657, 1133, 1572, 1635, 1875
BtsIMutI CAGTG 8 cut(s) 192, 657, 1133, 1339, 1572, 1611, 1635, 1875
BtuMI TCGCGA 1 cut(s) 774
Cac8I GCNNGC 3 cut(s) 294, 738, 1869
CfoI GCGC 3 cut(s) 591, 1213, 1449
Cfr13I GGNCC 3 cut(s) 105, 834, 1406
Cfr9I CCCGGG 1 cut(s) 1643
CseI GACGC 1 cut(s) 336
Csp6I GTAC 5 cut(s) 197, 445, 682, 1190, 1786
CviQI GTAC 5 cut(s) 197, 445, 682, 1190, 1786
DdeI CTNAG 4 cut(s) 77, 1197, 1578, 1814
DpnI GATC 7 cut(s) 85, 432, 554, 604, 612, 938, 1766
DpnII GATC 7 cut(s) 83, 430, 552, 602, 610, 936, 1764
DraIII CACNNNGTG 1 cut(s) 340
DrdI GACNNNNNNGTC 1 cut(s) 662
DseDI GACNNNNNNGTC 1 cut(s) 662
EaeI YGGCCR 3 cut(s) 313, 1608, 1860
Eam1104I CTCTTC 4 cut(s) 153, 1090, 1283, 1523
EarI CTCTTC 4 cut(s) 153, 1090, 1283, 1523
EciI GGCGGA 1 cut(s) 238
Ecl136II GAGCTC 2 cut(s) 976, 1516
Eco130I CCWWGG 2 cut(s) 297, 1050
Eco24I GRGCYC 2 cut(s) 978, 1518
Eco31I GGTCTC 1 cut(s) 1929
Eco32I GATATC 1 cut(s) 1219
Eco47I GGWCC 2 cut(s) 834, 1406
Eco53kI GAGCTC 2 cut(s) 976, 1516
Eco57I CTGAAG 1 cut(s) 834
Eco81I CCTNAGG 1 cut(s) 1197
Eco88I CYCGRG 3 cut(s) 236, 924, 1643
EcoICRI GAGCTC 2 cut(s) 976, 1516
EcoNI CCTNNNNNAGG 1 cut(s) 335
EcoRV GATATC 1 cut(s) 1219
EcoT14I CCWWGG 2 cut(s) 297, 1050
EcoT38I GRGCYC 2 cut(s) 978, 1518
ErhI CCWWGG 2 cut(s) 297, 1050
FaqI GGGAC 1 cut(s) 847
FauI CCCGC 2 cut(s) 444, 1981
FauNDI CATATG 1 cut(s) 1245
FbaI TGATCA 1 cut(s) 430
FblI GTMKAC 1 cut(s) 765
FokI GGATG 5 cut(s) 314, 437, 467, 1179, 1453
FriOI GRGCYC 2 cut(s) 978, 1518
FspBI CTAG 9 cut(s) 279, 417, 468, 474, 503, 737, 1149, 1604, 1853
GlaI GCGC 3 cut(s) 590, 1212, 1448
GsuI CTGGAG 1 cut(s) 326
HaeII RGCGCY 1 cut(s) 592
HaeIII GGCC 6 cut(s) 94, 107, 315, 922, 1610, 1862
HapII CCGG 4 cut(s) 66, 219, 1155, 1644
HgaI GACGC 1 cut(s) 336
HhaI GCGC 3 cut(s) 591, 1213, 1449
Hin1I GRCGYC 1 cut(s) 347
Hin6I GCGC 3 cut(s) 589, 1211, 1447
HinP1I GCGC 3 cut(s) 589, 1211, 1447
HincII GTYRAC 2 cut(s) 1229, 1317
HindII GTYRAC 2 cut(s) 1229, 1317
HindIII AAGCTT 1 cut(s) 778
HinfI GANTC 4 cut(s) 79, 874, 927, 1897
HpaI GTTAAC 1 cut(s) 1317
HpaII CCGG 4 cut(s) 66, 219, 1155, 1644
HphI GGTGA 3 cut(s) 589, 866, 1100
Hpy166II GTNNAC 4 cut(s) 766, 1229, 1317, 1875
Hpy188I TCNGA 4 cut(s) 78, 814, 973, 1679
Hpy188III TCNNGA 9 cut(s) 244, 351, 474, 773, 940, 1385, 1762, 1768, 1894
Hpy8I GTNNAC 4 cut(s) 766, 1229, 1317, 1875
Hpy99I CGWCG 4 cut(s) 191, 209, 233, 352
HpyAV CCTTC 3 cut(s) 737, 766, 1287
HpyCH4III ACNGT 7 cut(s) 196, 539, 854, 1334, 1594, 1615, 1885
HpyCH4IV ACGT 6 cut(s) 204, 231, 1396, 1435, 1754, 1935
HpyF3I CTNAG 4 cut(s) 77, 1197, 1578, 1814
HpySE526I ACGT 6 cut(s) 204, 231, 1396, 1435, 1754, 1935
Hsp92I GRCGYC 1 cut(s) 347
HspAI GCGC 3 cut(s) 589, 1211, 1447
Ksp22I TGATCA 1 cut(s) 430
KspAI GTTAAC 1 cut(s) 1317
Kzo9I GATC 7 cut(s) 83, 430, 552, 602, 610, 936, 1764
LguI GCTCTTC 1 cut(s) 1523
LmnI GCTCC 4 cut(s) 225, 973, 1513, 1645
Lsp1109I GCAGC 9 cut(s) 539, 972, 1063, 1348, 1610, 1637, 1806, 1927, 1984
LweI GCATC 5 cut(s) 454, 735, 780, 1267, 1363
MaeI CTAG 9 cut(s) 279, 417, 468, 474, 503, 737, 1149, 1604, 1853
MaeII ACGT 6 cut(s) 204, 231, 1396, 1435, 1754, 1935
MaeIII GTNAC 7 cut(s) 256, 285, 539, 1501, 1649, 1750, 1855
MalI GATC 7 cut(s) 85, 432, 554, 604, 612, 938, 1766
MbiI CCGCTC 2 cut(s) 451, 1813
MboI GATC 7 cut(s) 83, 430, 552, 602, 610, 936, 1764
MfeI CAATTG 2 cut(s) 731, 1418
MflI RGATCY 1 cut(s) 602
MhlI GDGCHC 4 cut(s) 237, 978, 1518, 1893
MlsI TGGCCA 2 cut(s) 315, 1610
MluNI TGGCCA 2 cut(s) 315, 1610
MlyI GAGTC 2 cut(s) 88, 936
MmeI TCCRAC 3 cut(s) 6, 1418, 1702
Mox20I TGGCCA 2 cut(s) 315, 1610
MscI TGGCCA 2 cut(s) 315, 1610
MseI TTAA 5 cut(s) 642, 1254, 1316, 2019, 2028
MslI CAYNNNNRTG 2 cut(s) 100, 1558
Msp20I TGGCCA 2 cut(s) 315, 1610
MspI CCGG 4 cut(s) 66, 219, 1155, 1644
MspR9I CCNGG 3 cut(s) 220, 1644, 1645
MunI CAATTG 2 cut(s) 731, 1418
MvnI CGCG 2 cut(s) 774, 1449
NciI CCSGG 3 cut(s) 220, 1644, 1645
NdeI CATATG 1 cut(s) 1245
NdeII GATC 7 cut(s) 83, 430, 552, 602, 610, 936, 1764
NheI GCTAGC 1 cut(s) 736
NlaIV GGNNCC 2 cut(s) 126, 835
NmeAIII GCCGAG 1 cut(s) 961
NmuCI GTSAC 5 cut(s) 256, 539, 1501, 1750, 1855
NruI TCGCGA 1 cut(s) 774
NspI RCATGY 2 cut(s) 296, 619
PaeI GCATGC 1 cut(s) 296
PaeR7I CTCGAG 2 cut(s) 236, 924
PciSI GCTCTTC 1 cut(s) 1523
PcsI WCGNNNNNNNCGW 2 cut(s) 393, 1760
PfeI GAWTC 2 cut(s) 874, 1897
PflFI GACNNNGTC 1 cut(s) 1921
PflMI CCANNNNNTGG 1 cut(s) 340
PleI GAGTC 2 cut(s) 87, 935
PpsI GAGTC 2 cut(s) 87, 935
PsiI TTATAA 1 cut(s) 1122
Psp124BI GAGCTC 2 cut(s) 978, 1518
PspN4I GGNNCC 2 cut(s) 126, 835
PspPI GGNCC 3 cut(s) 105, 834, 1406
PspXI VCTCGAGB 2 cut(s) 236, 924
PstI CTGCAG 1 cut(s) 1366
PsuI RGATCY 1 cut(s) 602
PsyI GACNNNGTC 1 cut(s) 1921
RruI TCGCGA 1 cut(s) 774
RsaI GTAC 5 cut(s) 198, 446, 683, 1191, 1787
RsaNI GTAC 5 cut(s) 197, 445, 682, 1190, 1786
RseI CAYNNNNRTG 2 cut(s) 100, 1558
SacI GAGCTC 2 cut(s) 978, 1518
SapI GCTCTTC 1 cut(s) 1523
SaqAI TTAA 5 cut(s) 642, 1254, 1316, 2019, 2028
Sau3AI GATC 7 cut(s) 83, 430, 552, 602, 610, 936, 1764
Sau96I GGNCC 3 cut(s) 105, 834, 1406
SchI GAGTC 2 cut(s) 88, 936
ScrFI CCNGG 3 cut(s) 220, 1644, 1645
SduI GDGCHC 4 cut(s) 237, 978, 1518, 1893
SfaNI GCATC 5 cut(s) 454, 735, 780, 1267, 1363
SfcI CTRYAG 3 cut(s) 520, 1362, 1881
Sfr274I CTCGAG 2 cut(s) 236, 924
SinI GGWCC 2 cut(s) 834, 1406
SlaI CTCGAG 2 cut(s) 236, 924
SmaI CCCGGG 1 cut(s) 1645
SmiMI CAYNNNNRTG 2 cut(s) 100, 1558
SmlI CTYRAG 2 cut(s) 236, 924
SmoI CTYRAG 2 cut(s) 236, 924
SphI GCATGC 1 cut(s) 296
SsiI CCGC 9 cut(s) 223, 365, 451, 837, 1486, 1629, 1662, 1811, 1988
SspI AATATT 1 cut(s) 1161
SspMI CTAG 9 cut(s) 279, 417, 468, 474, 503, 737, 1149, 1604, 1853
SstI GAGCTC 2 cut(s) 978, 1518
StyD4I CCNGG 3 cut(s) 218, 1642, 1643
StyI CCWWGG 2 cut(s) 297, 1050
TaaI ACNGT 7 cut(s) 196, 539, 854, 1334, 1594, 1615, 1885
TaiI ACGT 6 cut(s) 207, 234, 1399, 1438, 1757, 1938
TatI WGTACW 2 cut(s) 196, 1785
TauI GCSGC 3 cut(s) 368, 1631, 1813
TfiI GAWTC 2 cut(s) 874, 1897
Tru1I TTAA 5 cut(s) 642, 1254, 1316, 2019, 2028
Tru9I TTAA 5 cut(s) 642, 1254, 1316, 2019, 2028
TscAI CASTG 8 cut(s) 199, 664, 1140, 1339, 1579, 1618, 1642, 1882
TseFI GTSAC 5 cut(s) 256, 539, 1501, 1750, 1855
TseI GCWGC 9 cut(s) 527, 985, 1076, 1361, 1598, 1625, 1819, 1940, 1972
Tsp45I GTSAC 5 cut(s) 256, 539, 1501, 1750, 1855
TspDTI ATGAA 6 cut(s) 157, 617, 788, 1535, 1548, 1889
TspGWI ACGGA 1 cut(s) 610
TspMI CCCGGG 1 cut(s) 1643
TspRI CASTG 8 cut(s) 199, 664, 1140, 1339, 1579, 1618, 1642, 1882
Tth111I GACNNNGTC 1 cut(s) 1921
Van91I CCANNNNNTGG 1 cut(s) 340
VpaK11BI GGWCC 2 cut(s) 834, 1406
XagI CCTNNNNNAGG 1 cut(s) 335
XapI RAATTY 1 cut(s) 1792
XbaI TCTAGA 1 cut(s) 473
XceI RCATGY 2 cut(s) 296, 619
XhoI CTCGAG 2 cut(s) 236, 924
XmaI CCCGGG 1 cut(s) 1643
XmiI GTMKAC 1 cut(s) 765
XspI CTAG 9 cut(s) 279, 417, 468, 474, 503, 737, 1149, 1604, 1853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.