MD05G1353600.v1.1

Ankyrin repeat-containing protein At3g12360-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000724
Physical Location & Seq
Reverse (-)
47010642 .. 47011337
696 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1353600.v1.1.491

Sequence Viewer

Length: 696 bp
ATGATAATTGTGTACGGGTGTTTGTGTCAGCGTATAAAAGAGATAGTACCGCCTCATTACATCATGCACCACAACAACAACAACCAGACAGCGGAGGAGTTTTTCAACGTTGAGCATGAAAAGCTTTTGAAGTCCGCACAAAAATGGGTAAAAGAAACGGCTCAATCATGCTCAACCGTGGCGGTGCTAGTGGCCACTGTGGTCTTTGCAGCCGCCTATGCCATTCCTGGGGGTTTTAACGACGAAAGTGGCCGTCCTATTTTCCAGGATAATCCTCTCTTTTTGCTCTTCACCTGCATGGACGTTGTGTCCATTACATGCTCACTATCTTCAGTGGCATTCTTTCTCTCTGTCCTCTCCTCCCCTCTCGAGTACCCACATTTCGTTAGCAGCATTCCTACCAAGATCATGACAGGATTCATCTTGCTCTTCTTCTCCATGGCAACCACCATGCTCGCCTTTGCTGCCACCATTTTGCTCTTGATTCGGGTCAAGAAGAAATGGACCGAGTCTTTACTTTACCCTATTGCCTTTTTTCCAGTCCCTTTTTTTGCCCTGCTTCAATTTCCTATGTATCAATCTCTCATAACCATGGTTCAGAAAATTCATGCCTGGTTTGAAAAACCCATTTGTGGCTTTCTTGGCTTACGCAATAAGAGATCGGGATCAATATGGGGGAAGAAAAAGGTGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

232

Amino Acids

26.17

Weight (kDa)

9.12

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 48 - 159 7.7e-23 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 302
Acc36I ACCTGC 1 cut(s) 302
AciI CCGC 5 cut(s) 50, 92, 135, 182, 213
AclI AACGTT 1 cut(s) 108
AclWI GGATC 1 cut(s) 673
AcoI YGGCCR 2 cut(s) 192, 250
AcsI RAATTY 1 cut(s) 603
AcuI CTGAAG 1 cut(s) 315
AfaI GTAC 3 cut(s) 14, 48, 374
AfiI CCNNNNNNNGG 3 cut(s) 91, 228, 632
AgsI TTSAA 4 cut(s) 106, 130, 563, 620
AhdI GACNNNNNGTC 1 cut(s) 307
AjnI CCWGG 3 cut(s) 226, 264, 611
AluBI AGCT 1 cut(s) 124
AluI AGCT 1 cut(s) 124
AlwI GGATC 1 cut(s) 673
Ama87I CYCGRG 1 cut(s) 368
AoxI GGCC 2 cut(s) 192, 250
ApeKI GCWGC 3 cut(s) 209, 390, 464
ApoI RAATTY 1 cut(s) 603
AspS9I GGNCC 1 cut(s) 504
AsuHPI GGTGA 1 cut(s) 283
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 1 cut(s) 504
BalI TGGCCA 1 cut(s) 194
BbvI GCAGC 3 cut(s) 221, 402, 451
BceAI ACGGC 2 cut(s) 174, 237
BciT130I CCWGG 3 cut(s) 228, 266, 613
BfaI CTAG 1 cut(s) 188
BfuAI ACCTGC 1 cut(s) 302
BisI GCNGC 4 cut(s) 210, 213, 391, 465
BlsI GCNGC 4 cut(s) 211, 214, 392, 466
Bme1390I CCNGG 3 cut(s) 228, 266, 613
Bme18I GGWCC 1 cut(s) 504
BmeRI GACNNNNNGTC 1 cut(s) 307
BmeT110I CYCGRG 1 cut(s) 368
BmgT120I GGNCC 1 cut(s) 504
BmrFI CCNGG 3 cut(s) 228, 266, 613
BsaBI GATNNNNATC 1 cut(s) 664
BsaJI CCNNGG 4 cut(s) 177, 227, 438, 591
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 228, 632
Bse1I ACTGG 1 cut(s) 539
Bse8I GATNNNNATC 1 cut(s) 664
BseBI CCWGG 3 cut(s) 228, 266, 613
BseDI CCNNGG 4 cut(s) 177, 227, 438, 591
BseJI GATNNNNATC 1 cut(s) 664
BseLI CCNNNNNNNGG 3 cut(s) 91, 228, 632
BseNI ACTGG 1 cut(s) 539
BseRI GAGGAG 2 cut(s) 110, 349
BseXI GCAGC 3 cut(s) 221, 402, 451
BshFI GGCC 2 cut(s) 194, 252
BsiHKCI CYCGRG 1 cut(s) 368
BslFI GGGAC 1 cut(s) 527
BslI CCNNNNNNNGG 3 cut(s) 91, 228, 632
BsmFI GGGAC 1 cut(s) 527
BsmI GAATGC 2 cut(s) 338, 393
BsnI GGCC 2 cut(s) 194, 252
BsoBI CYCGRG 1 cut(s) 368
Bsp143I GATC 3 cut(s) 405, 659, 665
Bsp19I CCATGG 2 cut(s) 438, 591
BspACI CCGC 5 cut(s) 50, 92, 135, 182, 213
BspANI GGCC 2 cut(s) 194, 252
BspHI TCATGA 1 cut(s) 408
BspMI ACCTGC 1 cut(s) 302
BspPI GGATC 1 cut(s) 673
BspQI GCTCTTC 2 cut(s) 293, 434
BsrI ACTGG 1 cut(s) 539
BssECI CCNNGG 4 cut(s) 177, 227, 438, 591
BssMI GATC 3 cut(s) 405, 659, 665
BssT1I CCWWGG 2 cut(s) 438, 591
Bst2UI CCWGG 3 cut(s) 228, 266, 613
Bst4CI ACNGT 2 cut(s) 178, 199
Bst6I CTCTTC 2 cut(s) 293, 434
BstC8I GCNNGC 1 cut(s) 456
BstDSI CCRYGG 3 cut(s) 177, 438, 591
BstKTI GATC 3 cut(s) 408, 662, 668
BstMBI GATC 3 cut(s) 405, 659, 665
BstMWI GCNNNNNNNGC 4 cut(s) 121, 218, 464, 642
BstNI CCWGG 3 cut(s) 228, 266, 613
BstNSI RCATGY 1 cut(s) 321
BstSCI CCNGG 3 cut(s) 226, 264, 611
BstV1I GCAGC 3 cut(s) 221, 402, 451
BsuRI GGCC 2 cut(s) 194, 252
BtgI CCRYGG 3 cut(s) 177, 438, 591
BtsIMutI CAGTG 2 cut(s) 195, 339
BveI ACCTGC 1 cut(s) 302
Cac8I GCNNGC 1 cut(s) 456
CciI TCATGA 1 cut(s) 408
Cfr13I GGNCC 1 cut(s) 504
Csp6I GTAC 3 cut(s) 13, 47, 373
CviJI RGCY 7 cut(s) 124, 161, 194, 212, 252, 636, 645
CviKI_1 RGCY 7 cut(s) 124, 161, 194, 212, 252, 636, 645
CviQI GTAC 3 cut(s) 13, 47, 373
DpnI GATC 3 cut(s) 407, 661, 667
DpnII GATC 3 cut(s) 405, 659, 665
DriI GACNNNNNGTC 1 cut(s) 307
EaeI YGGCCR 2 cut(s) 192, 250
Eam1104I CTCTTC 2 cut(s) 293, 434
Eam1105I GACNNNNNGTC 1 cut(s) 307
EarI CTCTTC 2 cut(s) 293, 434
Eco130I CCWWGG 2 cut(s) 438, 591
Eco47I GGWCC 1 cut(s) 504
Eco57I CTGAAG 1 cut(s) 315
Eco88I CYCGRG 1 cut(s) 368
EcoRII CCWGG 3 cut(s) 226, 264, 611
EcoT14I CCWWGG 2 cut(s) 438, 591
ErhI CCWWGG 2 cut(s) 438, 591
FaqI GGGAC 1 cut(s) 527
Fnu4HI GCNGC 4 cut(s) 210, 213, 391, 465
Fsp4HI GCNGC 4 cut(s) 210, 213, 391, 465
FspBI CTAG 1 cut(s) 188
GluI GCNGC 4 cut(s) 210, 213, 391, 465
HaeIII GGCC 2 cut(s) 194, 252
HindIII AAGCTT 1 cut(s) 122
HinfI GANTC 3 cut(s) 417, 484, 509
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 1 cut(s) 600
Hpy188III TCNNGA 5 cut(s) 368, 409, 481, 493, 663
Hpy8I GTNNAC 1 cut(s) 13
Hpy99I CGWCG 1 cut(s) 245
HpyCH4III ACNGT 2 cut(s) 178, 199
HpyCH4IV ACGT 2 cut(s) 108, 303
HpyCH4V TGCA 3 cut(s) 67, 209, 297
HpyF10VI GCNNNNNNNGC 4 cut(s) 121, 218, 464, 642
HpySE526I ACGT 2 cut(s) 108, 303
Kzo9I GATC 3 cut(s) 405, 659, 665
LguI GCTCTTC 2 cut(s) 293, 434
Lsp1109I GCAGC 3 cut(s) 221, 402, 451
MaeI CTAG 1 cut(s) 188
MaeII ACGT 2 cut(s) 108, 303
MalI GATC 3 cut(s) 407, 661, 667
MboI GATC 3 cut(s) 405, 659, 665
MboII GAAGA 6 cut(s) 280, 321, 421, 424, 508, 691
MlsI TGGCCA 1 cut(s) 194
MluCI AATT 3 cut(s) 6, 563, 603
MluNI TGGCCA 1 cut(s) 194
MlyI GAGTC 1 cut(s) 518
MnlI CCTC 6 cut(s) 63, 88, 285, 365, 370, 375
Mox20I TGGCCA 1 cut(s) 194
MscI TGGCCA 1 cut(s) 194
MseI TTAA 1 cut(s) 237
MslI CAYNNNNRTG 3 cut(s) 142, 296, 590
Msp20I TGGCCA 1 cut(s) 194
MspA1I CMGCKG 1 cut(s) 92
MspR9I CCNGG 3 cut(s) 228, 266, 613
Mva1269I GAATGC 2 cut(s) 338, 393
MvaI CCWGG 3 cut(s) 228, 266, 613
MwoI GCNNNNNNNGC 4 cut(s) 121, 218, 464, 642
NcoI CCATGG 2 cut(s) 438, 591
NdeII GATC 3 cut(s) 405, 659, 665
NspI RCATGY 1 cut(s) 321
PaeR7I CTCGAG 1 cut(s) 368
PagI TCATGA 1 cut(s) 408
PaqCI CACCTGC 1 cut(s) 302
PciSI GCTCTTC 2 cut(s) 293, 434
PctI GAATGC 2 cut(s) 338, 393
PfeI GAWTC 2 cut(s) 417, 484
PflFI GACNNNGTC 1 cut(s) 508
PfoI TCCNGGA 1 cut(s) 264
PkrI GCNGC 4 cut(s) 211, 214, 392, 466
PleI GAGTC 1 cut(s) 517
PpsI GAGTC 1 cut(s) 517
Psp1406I AACGTT 1 cut(s) 108
Psp6I CCWGG 3 cut(s) 226, 264, 611
PspGI CCWGG 3 cut(s) 226, 264, 611
PspPI GGNCC 1 cut(s) 504
PsyI GACNNNGTC 1 cut(s) 508
RsaI GTAC 3 cut(s) 14, 48, 374
RsaNI GTAC 3 cut(s) 13, 47, 373
RseI CAYNNNNRTG 3 cut(s) 142, 296, 590
SapI GCTCTTC 2 cut(s) 293, 434
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 4 cut(s) 210, 213, 391, 465
Sau3AI GATC 3 cut(s) 405, 659, 665
Sau96I GGNCC 1 cut(s) 504
SchI GAGTC 1 cut(s) 518
ScrFI CCNGG 3 cut(s) 228, 266, 613
SetI ASST 5 cut(s) 111, 126, 296, 306, 690
Sfr274I CTCGAG 1 cut(s) 368
SinI GGWCC 1 cut(s) 504
SlaI CTCGAG 1 cut(s) 368
SmiMI CAYNNNNRTG 3 cut(s) 142, 296, 590
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 3 cut(s) 6, 563, 603
SsiI CCGC 5 cut(s) 50, 92, 135, 182, 213
SspMI CTAG 1 cut(s) 188
StyD4I CCNGG 3 cut(s) 226, 264, 611
StyI CCWWGG 2 cut(s) 438, 591
TaaI ACNGT 2 cut(s) 178, 199
TaiI ACGT 2 cut(s) 111, 306
TaqI TCGA 1 cut(s) 369
TaqII GACCGA 1 cut(s) 521
TasI AATT 3 cut(s) 6, 563, 603
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 2 cut(s) 417, 484
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 2 cut(s) 202, 339
TseI GCWGC 3 cut(s) 209, 390, 464
TspDTI ATGAA 3 cut(s) 132, 409, 596
TspRI CASTG 2 cut(s) 202, 339
Tth111I GACNNNGTC 1 cut(s) 508
VpaK11BI GGWCC 1 cut(s) 504
XapI RAATTY 1 cut(s) 603
XceI RCATGY 1 cut(s) 321
XhoI CTCGAG 1 cut(s) 368
XspI CTAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.