pycom05g32070

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000724
Physical Location & Seq
Forward (+)
31606941 .. 31607952
1012 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32070.1

Sequence Viewer

Length: 804 bp
ATGTCGATGTTGAATGAGAGGATAGACCACCAAGGAAACACCTTATTGCACCAGGCTGCAGATAGGAGTTACTACTCTATATCATTGACTCAGCAATTAATAGGCCCTGCCACGCAATTGCAAGAAGAGCTGCACTGGTTTGAGCGTGTAAAAGGGATAATACCGCCTCATTACACCATTCACCACAACCATTACAATCAGACAGCGGACGACTTGTTCAACTATTGGCATGACGAACTTTTGGAGGAAGCACAAAAATGGGTAAAAGACACGGCTCAGTCATGCTCAACCGTGGCGGTGCTAGTTGCCACTGTAGTCTTTGCAGCTGCCTACACCATTCCCGGGGGGACTAATGGCCAAAATGGCGTTCCTCTTTTCCAGATCGATCCTCTCTTTTTGCTCTTCACCTGCATGGACGTTGTGGCCATCGCCAGCTCATTATCTTCTGTGGCATTCTTTCTCTCGGTCCTCCCCTCCCCTCTCGAGTACCCACTTTTTGTTAGCAGCATTCCTAACAAGATCATGGCAGGTTTCGTCTTGCTCTTCATTTCCATGGCAACAACCATGCTCGCCTTTGCTGCCACCATTTTGTTGTTGATTCGCGTCGAGAAGAAATGGACCAAGTCTCTACTTTACCCTATTGCCTTTTTCCCAGTCCCTTTATTCGGCTTGCTTCAATTTCCTATGTATCATTCCTTCAAAGTCGTGTTTCATGAAATTAATGCCTGGTTTTTCAAACCCTTACTCGACTTGCTTGGCTTTCCCAAGAAGAGATCAATATGGGGCAAGAGTAAGGCGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

268

Amino Acids

30.23

Weight (kDa)

6.54

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 86 - 197 1.3e-20 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01330 FvH4_3g01340
malus_domestica MD05G1025600.v1.1 MD05G1025700.v1.1 MD05G1026000.v1.1 MD05G1353100.v1.1 MD05G1353500.v1.1 MD05G1353600.v1.1 MD05G1353800.v1.1 MD05G1354800.v1.1 MD09G1059700.v1.1 MD09G1059800.v1.1 MD10G1327600.v1.1 MD10G1327700.v1.1 MD10G1327800.v1.1
prunus_persica Prupe.4G014100_v2.0.a1 Prupe.4G014100_v2.0.a1 Prupe.4G014200_v2.0.a1 Prupe.4G014300_v2.0.a1 Prupe.4G014400_v2.0.a1 Prupe.7G089200_v2.0.a1 Prupe.8G021100_v2.0.a1 Prupe.8G029400_v2.0.a1 Prupe.8G029600_v2.0.a1 Prupe.8G029900_v2.0.a1 Prupe.8G030300_v2.0.a1
pyrus_communis pycom05g01740 pycom05g01780 pycom05g32030 pycom05g32040 pycom05g32050 pycom05g32070 pycom05g32080 pycom05g32090 pycom10g27710 pycom10g27740 pycom10g27760 pycom13g16890 pycom17g07270 pycom17g07280 pycom17g07290
rosa_chinensis RchiOBHm_Chr5g0001901 RchiOBHm_Chr5g0001921 RchiOBHm_Chr5g0001941
rosa_laevigata RLG00000030980 RLG00000030981 RLG00000030984
rosa_multiflora Rmu_sc0000378.1_g000012 Rmu_sc0000378.1_g000015 Rmu_sc0005788.1_g000001 Rmu_sc0006008.1_g000002
rosa_roxburghii Rroxscaffold_1G00071320 Rroxscaffold_1G00071340 Rroxscaffold_1G00071350 Rroxscaffold_1G00074390 Rroxscaffold_1G00074410 Rroxscaffold_1G00074460
rosa_rugosa Rorug04G0393700 Rorug04G0393800 Rorug04G0393900 Rorug04G0394000
rosa_samantha Rh5AG015000 Rh5BG018400 Rh5BG018500 Rh5BG018600 Rh5BG018700 Rh5CG016100 Rh5DG016600 Rh5DG016800 Rh5DG017000
rosa_wichuraiana Rw5G001450 Rw5G001460 Rw5G001480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 416
Acc36I ACCTGC 2 cut(s) 416, 518
AccII CGCG 1 cut(s) 603
AciI CCGC 4 cut(s) 164, 206, 296, 797
AclWI GGATC 1 cut(s) 380
AcoI YGGCCR 2 cut(s) 355, 423
AfaI GTAC 1 cut(s) 488
AfiI CCNNNNNNNGG 2 cut(s) 342, 665
AgsI TTSAA 5 cut(s) 13, 220, 677, 700, 736
AjnI CCWGG 2 cut(s) 51, 725
AjuI GAANNNNNNNTTGG 2 cut(s) 351, 383
AloI GAACNNNNNNTCC 2 cut(s) 200, 232
AluBI AGCT 3 cut(s) 130, 326, 435
AluI AGCT 3 cut(s) 130, 326, 435
Alw26I GTCTC 1 cut(s) 630
AlwI GGATC 1 cut(s) 380
Ama87I CYCGRG 2 cut(s) 341, 482
AoxI GGCC 3 cut(s) 103, 355, 423
ApeKI GCWGC 6 cut(s) 56, 130, 323, 326, 504, 578
AseI ATTAAT 2 cut(s) 98, 720
AspS9I GGNCC 3 cut(s) 104, 466, 618
AsuC2I CCSGG 2 cut(s) 342, 343
AsuHPI GGTGA 2 cut(s) 173, 397
AvaI CYCGRG 2 cut(s) 341, 482
AvaII GGWCC 2 cut(s) 466, 618
BalI TGGCCA 2 cut(s) 357, 425
BarI GAAGNNNNNNTAC 2 cut(s) 680, 712
BbvI GCAGC 6 cut(s) 43, 117, 313, 335, 516, 565
BccI CCATC 1 cut(s) 434
BceAI ACGGC 1 cut(s) 288
BciT130I CCWGG 2 cut(s) 53, 727
BcnI CCSGG 2 cut(s) 342, 343
BcoDI GTCTC 1 cut(s) 630
BfaI CTAG 1 cut(s) 302
BfmI CTRYAG 2 cut(s) 57, 312
BfuAI ACCTGC 2 cut(s) 416, 518
BglI GCCNNNNNGGC 1 cut(s) 363
BisI GCNGC 6 cut(s) 57, 131, 324, 327, 505, 579
BlsI GCNGC 6 cut(s) 58, 132, 325, 328, 506, 580
Bme1390I CCNGG 4 cut(s) 53, 342, 343, 727
Bme18I GGWCC 2 cut(s) 466, 618
BmeT110I CYCGRG 2 cut(s) 341, 482
BmgT120I GGNCC 3 cut(s) 104, 466, 618
BmrFI CCNGG 4 cut(s) 53, 342, 343, 727
BmrI ACTGGG 1 cut(s) 647
BmuI ACTGGG 1 cut(s) 647
BpuMI CCSGG 2 cut(s) 342, 343
Bsa29I ATCGAT 1 cut(s) 384
BsaJI CCNNGG 5 cut(s) 31, 291, 341, 342, 552
Bsc4I CCNNNNNNNGG 2 cut(s) 342, 665
Bse1I ACTGG 2 cut(s) 140, 653
BseBI CCWGG 2 cut(s) 53, 727
BseCI ATCGAT 1 cut(s) 384
BseDI CCNNGG 5 cut(s) 31, 291, 341, 342, 552
BseLI CCNNNNNNNGG 2 cut(s) 342, 665
BseMII CTCAG 2 cut(s) 104, 290
BseNI ACTGG 2 cut(s) 140, 653
BseXI GCAGC 6 cut(s) 43, 117, 313, 335, 516, 565
BsgI GTGCAG 1 cut(s) 116
Bsh1236I CGCG 1 cut(s) 603
BshFI GGCC 3 cut(s) 105, 357, 425
BshVI ATCGAT 1 cut(s) 384
BsiHKCI CYCGRG 2 cut(s) 341, 482
BsiSI CCGG 1 cut(s) 342
BslFI GGGAC 2 cut(s) 361, 641
BslI CCNNNNNNNGG 2 cut(s) 342, 665
BsmAI GTCTC 1 cut(s) 630
BsmFI GGGAC 2 cut(s) 361, 641
BsmI GAATGC 2 cut(s) 452, 507
BsnI GGCC 3 cut(s) 105, 357, 425
BsoBI CYCGRG 2 cut(s) 341, 482
Bsp143I GATC 4 cut(s) 381, 385, 519, 773
Bsp19I CCATGG 1 cut(s) 552
BspACI CCGC 4 cut(s) 164, 206, 296, 797
BspANI GGCC 3 cut(s) 105, 357, 425
BspCNI CTCAG 2 cut(s) 103, 289
BspDI ATCGAT 1 cut(s) 384
BspFNI CGCG 1 cut(s) 603
BspHI TCATGA 1 cut(s) 712
BspMAI CTGCAG 1 cut(s) 61
BspMI ACCTGC 2 cut(s) 416, 518
BspPI GGATC 1 cut(s) 380
BspQI GCTCTTC 3 cut(s) 120, 407, 548
BsrI ACTGG 2 cut(s) 140, 653
BssECI CCNNGG 5 cut(s) 31, 291, 341, 342, 552
BssMI GATC 4 cut(s) 381, 385, 519, 773
BssT1I CCWWGG 2 cut(s) 31, 552
Bst2UI CCWGG 2 cut(s) 53, 727
Bst4CI ACNGT 2 cut(s) 292, 313
Bst6I CTCTTC 4 cut(s) 120, 407, 548, 764
BstC8I GCNNGC 3 cut(s) 433, 570, 671
BstDEI CTNAG 2 cut(s) 90, 276
BstDSI CCRYGG 2 cut(s) 291, 552
BstFNI CGCG 1 cut(s) 603
BstKTI GATC 4 cut(s) 384, 388, 522, 776
BstMAI GTCTC 1 cut(s) 630
BstMBI GATC 4 cut(s) 381, 385, 519, 773
BstMWI GCNNNNNNNGC 3 cut(s) 127, 363, 578
BstNI CCWGG 2 cut(s) 53, 727
BstSCI CCNGG 4 cut(s) 51, 340, 341, 725
BstSFI CTRYAG 2 cut(s) 57, 312
BstUI CGCG 1 cut(s) 603
BstV1I GCAGC 6 cut(s) 43, 117, 313, 335, 516, 565
Bsu15I ATCGAT 1 cut(s) 384
BsuRI GGCC 3 cut(s) 105, 357, 425
BsuTUI ATCGAT 1 cut(s) 384
BtgI CCRYGG 2 cut(s) 291, 552
BtgZI GCGATG 1 cut(s) 412
BtsIMutI CAGTG 2 cut(s) 133, 309
BveI ACCTGC 2 cut(s) 416, 518
Cac8I GCNNGC 3 cut(s) 433, 570, 671
CciI TCATGA 1 cut(s) 712
Cfr13I GGNCC 3 cut(s) 104, 466, 618
Cfr9I CCCGGG 1 cut(s) 341
ClaI ATCGAT 1 cut(s) 384
CseI GACGC 1 cut(s) 592
Csp6I GTAC 1 cut(s) 487
CviAII CATG 7 cut(s) 230, 282, 412, 523, 553, 565, 713
CviQI GTAC 1 cut(s) 487
DdeI CTNAG 2 cut(s) 90, 276
DpnI GATC 4 cut(s) 383, 387, 521, 775
DpnII GATC 4 cut(s) 381, 385, 519, 773
EaeI YGGCCR 2 cut(s) 355, 423
Eam1104I CTCTTC 4 cut(s) 120, 407, 548, 764
EarI CTCTTC 4 cut(s) 120, 407, 548, 764
Eco130I CCWWGG 2 cut(s) 31, 552
Eco47I GGWCC 2 cut(s) 466, 618
Eco88I CYCGRG 2 cut(s) 341, 482
EcoO109I RGGNCCY 1 cut(s) 104
EcoRII CCWGG 2 cut(s) 51, 725
EcoT14I CCWWGG 2 cut(s) 31, 552
ErhI CCWWGG 2 cut(s) 31, 552
FaeI CATG 7 cut(s) 233, 285, 415, 526, 556, 568, 716
FaqI GGGAC 2 cut(s) 361, 641
FatI CATG 7 cut(s) 229, 281, 411, 522, 552, 564, 712
Fnu4HI GCNGC 6 cut(s) 57, 131, 324, 327, 505, 579
Fsp4HI GCNGC 6 cut(s) 57, 131, 324, 327, 505, 579
FspBI CTAG 1 cut(s) 302
GluI GCNGC 6 cut(s) 57, 131, 324, 327, 505, 579
HaeIII GGCC 3 cut(s) 105, 357, 425
HapII CCGG 1 cut(s) 342
HgaI GACGC 1 cut(s) 592
Hin1II CATG 7 cut(s) 233, 285, 415, 526, 556, 568, 716
HinfI GANTC 2 cut(s) 88, 598
HpaII CCGG 1 cut(s) 342
HphI GGTGA 2 cut(s) 173, 397
Hpy188I TCNGA 1 cut(s) 201
Hpy188III TCNNGA 4 cut(s) 379, 482, 607, 713
Hpy99I CGWCG 1 cut(s) 608
HpyAV CCTTC 1 cut(s) 706
HpyCH4III ACNGT 2 cut(s) 292, 313
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 6 cut(s) 49, 59, 121, 133, 323, 411
HpyF10VI GCNNNNNNNGC 3 cut(s) 127, 363, 578
HpyF3I CTNAG 2 cut(s) 90, 276
HpySE526I ACGT 1 cut(s) 417
Hsp92II CATG 7 cut(s) 233, 285, 415, 526, 556, 568, 716
Kzo9I GATC 4 cut(s) 381, 385, 519, 773
LguI GCTCTTC 3 cut(s) 120, 407, 548
Lsp1109I GCAGC 6 cut(s) 43, 117, 313, 335, 516, 565
MaeI CTAG 1 cut(s) 302
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 1 cut(s) 68
MalI GATC 4 cut(s) 383, 387, 521, 775
MboI GATC 4 cut(s) 381, 385, 519, 773
MboII GAAGA 6 cut(s) 137, 394, 435, 535, 622, 781
MfeI CAATTG 1 cut(s) 116
MlsI TGGCCA 2 cut(s) 357, 425
MluCI AATT 4 cut(s) 95, 116, 677, 717
MluNI TGGCCA 2 cut(s) 357, 425
MlyI GAGTC 1 cut(s) 82
MnlI CCTC 8 cut(s) 12, 177, 238, 381, 399, 479, 484, 489
Mox20I TGGCCA 2 cut(s) 357, 425
MscI TGGCCA 2 cut(s) 357, 425
MseI TTAA 2 cut(s) 98, 720
MslI CAYNNNNRTG 3 cut(s) 256, 410, 551
Msp20I TGGCCA 2 cut(s) 357, 425
MspA1I CMGCKG 2 cut(s) 206, 326
MspI CCGG 1 cut(s) 342
MspR9I CCNGG 4 cut(s) 53, 342, 343, 727
MunI CAATTG 1 cut(s) 116
Mva1269I GAATGC 2 cut(s) 452, 507
MvaI CCWGG 2 cut(s) 53, 727
MvnI CGCG 1 cut(s) 603
MwoI GCNNNNNNNGC 3 cut(s) 127, 363, 578
NciI CCSGG 2 cut(s) 342, 343
NcoI CCATGG 1 cut(s) 552
NdeII GATC 4 cut(s) 381, 385, 519, 773
NlaIII CATG 7 cut(s) 233, 285, 415, 526, 556, 568, 716
PaeR7I CTCGAG 1 cut(s) 482
PagI TCATGA 1 cut(s) 712
PaqCI CACCTGC 1 cut(s) 416
PciSI GCTCTTC 3 cut(s) 120, 407, 548
PctI GAATGC 2 cut(s) 452, 507
PfeI GAWTC 1 cut(s) 598
PflFI GACNNNGTC 1 cut(s) 622
PkrI GCNGC 6 cut(s) 58, 132, 325, 328, 506, 580
PleI GAGTC 1 cut(s) 82
PpsI GAGTC 1 cut(s) 82
PshBI ATTAAT 2 cut(s) 98, 720
Psp6I CCWGG 2 cut(s) 51, 725
PspGI CCWGG 2 cut(s) 51, 725
PspPI GGNCC 3 cut(s) 104, 466, 618
PstI CTGCAG 1 cut(s) 61
PsyI GACNNNGTC 1 cut(s) 622
PvuII CAGCTG 1 cut(s) 326
RsaI GTAC 1 cut(s) 488
RsaNI GTAC 1 cut(s) 487
RseI CAYNNNNRTG 3 cut(s) 256, 410, 551
SapI GCTCTTC 3 cut(s) 120, 407, 548
SaqAI TTAA 2 cut(s) 98, 720
SatI GCNGC 6 cut(s) 57, 131, 324, 327, 505, 579
Sau3AI GATC 4 cut(s) 381, 385, 519, 773
Sau96I GGNCC 3 cut(s) 104, 466, 618
SchI GAGTC 1 cut(s) 82
ScrFI CCNGG 4 cut(s) 53, 342, 343, 727
SetI ASST 7 cut(s) 44, 132, 328, 410, 420, 437, 532
SfcI CTRYAG 2 cut(s) 57, 312
Sfr274I CTCGAG 1 cut(s) 482
SinI GGWCC 2 cut(s) 466, 618
SlaI CTCGAG 1 cut(s) 482
SmaI CCCGGG 1 cut(s) 343
SmiMI CAYNNNNRTG 3 cut(s) 256, 410, 551
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
Sse9I AATT 4 cut(s) 95, 116, 677, 717
SsiI CCGC 4 cut(s) 164, 206, 296, 797
SspMI CTAG 1 cut(s) 302
StyD4I CCNGG 4 cut(s) 51, 340, 341, 725
StyI CCWWGG 2 cut(s) 31, 552
TaaI ACNGT 2 cut(s) 292, 313
TaiI ACGT 1 cut(s) 420
TaqI TCGA 5 cut(s) 5, 384, 483, 606, 747
TaqII GACCGA 1 cut(s) 454
TasI AATT 4 cut(s) 95, 116, 677, 717
TfiI GAWTC 1 cut(s) 598
Tru1I TTAA 2 cut(s) 98, 720
Tru9I TTAA 2 cut(s) 98, 720
TscAI CASTG 2 cut(s) 140, 316
TseI GCWGC 6 cut(s) 56, 130, 323, 326, 504, 578
TspDTI ATGAA 3 cut(s) 535, 701, 729
TspMI CCCGGG 1 cut(s) 341
TspRI CASTG 2 cut(s) 140, 316
Tth111I GACNNNGTC 1 cut(s) 622
VpaK11BI GGWCC 2 cut(s) 466, 618
VspI ATTAAT 2 cut(s) 98, 720
XhoI CTCGAG 1 cut(s) 482
XmaI CCCGGG 1 cut(s) 341
XspI CTAG 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.