MD12G1255700.v1.1

MuDR family transposase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000251
Physical Location & Seq
Forward (+)
32355376 .. 32358695
3320 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1255700.v1.1.491

Sequence Viewer

Length: 1791 bp
ATGGAAGATCATTCGTTAGCTGTGCAGAACGATTCTGTTGCCTTGACGGAATATGCTTTAGACTTGGCTATTGGGCAAGAATTTCCTGATGTTGAAACTTGCAGAAGAAGATTGAAGAACATTGCTTTAGTACAACATTTTGATCTTCGTATAGTGAAATCAGATCGCAGCCGGTTTATAGCCAAGTGCTCCAAAGAAGGTTGCCCATGGCGTGTCCATGTGGCAAAATGTCCCGGAGTTCCAACCTTTACAGTAAGAACCCTGCATGGTGAGCATACCTGTGAAGGAGTTCTCAACCTTCATCATCAGCAAGCATCAGTAGGTTGGGTTGCTAGGTCTGTAGAAGCACGGATTCGGGATAACCCACGGTATAAACCAAAAGAGATCCTGCAAGATATCCGTGATCAGCATGGAGTTGCTGTTTCTTACATGCAAGCTTGGCGTGGCAAGGAACGTAGCATGGCAGCACTTCATGGAACTTTTGAAGAAGGGTATCGCCTTCTTCCTGCATACTGTGAGCAAATAAAGAAAACCAACCCTGGAAGCATTGCTTCAGTTTTTGCCACTGGACAAGAAAATTGCTTCCACCGTCTCTTCATTTCTTACCGTGCATCAATTTATGGGTTTATACATGCTTGTAGGCCACTTTTGGAACTTGATAGAGCGCACCTCAAAGGCAAATACCTGGGTTCCGTACTTTGTGCTGCAGCTGTTGATGCTGATGATACATTATTTCCTCTGGCAATAGCTATCGTTGATGTGGAAAGCGACGAAAATTGGATGTGGTTTATGTCAGAATTGCGAAAGCTTCTTGCAGTAAACACTGACAACATGCCTAGGCTAACAATACTCTCTGAGAGACAAAGGGGTGTTGTAGAGGCAGTGGAAACTCATTTTCCTAGTGCCTTCCATGGTTTTTGTCTGCGTTATGTTAGTGAAAATTTTCGTGATACCTTTAAAAATGCGAAGTTGGTGAATATCTTCTGGAATGCTGTTTATGCTCTCACTGCAGTTGAATTTGACAGCAAGATTGCAGAGATGGCAGAGATCTCACAAGACGTGATACCATGGTTTCAACACTTCCCTCCCCAACTTTGGGCTGTAGCATATTTTGAAGGAGTGCGATATGGCCATTTTACTCTCGGTGTTACAGAGCTGTTGTATAATTGGGCTCTTGAATGCCATGAGCTCCCTATTGTGCAAATGATGGAGCATATTCGTGATCAGTTATCATCATGGTATAATAACCGCCGGGATATGGGAATGAGTTGGACATCAATTCTGGTACCATCTGCTGAGAAGCGGATTCTAGAGGCAATGGCCGATGCTAGATGCTACCAAGTACTTCGTGCAAATGAAGTCGAATTTGAGATTGTGTCTACTGAGCGGACAAATATCGTGGATATACAAACTCGCGTGTGCTCATGTCGCCGCTGGCATATATATGGTCTACCTTGTGCACATGCTGCTGCCGCACTTATGTCCTGTGGACACAATGTCCATTTGTTTGCTGAGCCTTGCTTCACAGTAGCAAGTTACCGAGAGACCTATTCGCAGCTGATTCATCCTATTCCGGATAGGAGCTTATGGAAGGAGCCAAGCGACGGAACAGAGGGGGGAAGCACCAATGTTGATATCATGATACGCCCACCCAAGACTCGGCGGCCACCTGGAAGACCCAAGAAGAAGGTCCTTCGTGTAGAGAACTTCAAGAGGCCGAAGCGAGTTGTTCAATGTGGTCGCTGCCATATGTTAGGACATTCTCAAAAGAAATGTACCTTACCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

597

Amino Acids

68.24

Weight (kDa)

8.4

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBD_Tnp_Mut PF03108 22 - 85 6.6e-21 MuDR family transposase
MULE PF10551 217 - 314 6.9e-14 MULE transposase domain
SWIM PF04434 467 - 497 2e-08 SWIM zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06740
fragaria_vesca FvH4_1g02210 FvH4_1g02210 FvH4_1g02210 FvH4_1g02210 FvH4_2g02692 FvH4_2g22241 FvH4_2g22241 FvH4_2g22241 FvH4_4g21561 FvH4_6g01063 FvH4_6g01063 FvH4_6g01063 FvH4_6g01063 FvH4_6g01063 FvH4_6g01063 FvH4_6g02501 FvH4_6g02501
malus_domestica MD02G1020500.v1.1 MD04G1227400.v1.1 MD04G1236400.v1.1 MD05G1203600.v1.1 MD10G1190900.v1.1 MD12G1244100.v1.1 MD12G1255700.v1.1 MD13G1140100.v1.1 MD15G1396600.v1.1 MD16G1135600.v1.1
prunus_persica Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.1G104800_v2.0.a1 Prupe.6G346500_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.6G356400_v2.0.a1 Prupe.7G251000_v2.0.a1 Prupe.7G251000_v2.0.a1 Prupe.7G251000_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G156200_v2.0.a1 Prupe.8G229900_v2.0.a1 Prupe.8G229900_v2.0.a1
pyrus_communis pycom02g01800 pycom02g16220 pycom04g20090 pycom04g21020 pycom04g21030 pycom05g19010 pycom10g16460 pycom12g18690 pycom12g22380 pycom12g23430 pycom13g12180 pycom15g14690 pycom15g23420 pycom15g35670 pycom16g11960
rosa_chinensis RchiOBHm_Chr2g0087301 RchiOBHm_Chr3g0448611 RchiOBHm_Chr3g0450381 RchiOBHm_Chr4g0427621 RchiOBHm_Chr6g0247111 RchiOBHm_Chr6g0289171 RchiOBHm_Chr7g0177141
rosa_laevigata RLG00000007221 RLG00000015852
rosa_multiflora Rmu_co8326617.1_g000001 Rmu_sc0003399.1_g000010
rosa_roxburghii Rroxscaffold_2G00153730 Rroxscaffold_5G00369250 Rroxscaffold_6G00426900 Rroxscaffold_6G00428110 Rroxscaffold_7G00179510 Rroxscaffold_7G00214340
rosa_rugosa Rorug01G0473800 Rorug01G0473900 Rorug01G0474000 Rorug02G0614200 Rorug02G0614300 Rorug02G0614400 Rorug02G0614500 Rorug02G0614600 Rorug02G0627200 Rorug02G0627300 Rorug02G0627400 Rorug02G0627500 Rorug05G0522400
rosa_samantha Rh2AG027500 Rh2BG026800 Rh2CG027900 Rh2DG027500 Rh3AG013600 Rh3AG027900 Rh3BG013400 Rh3BG028400 Rh3CG012500 Rh3CG027200 Rh3DG014200 Rh3DG028300 Rh4AG275400 Rh4BG281300 Rh4CG296600 Rh4DG278600 Rh6AG037400 Rh6AG037700 Rh6AG312600 Rh6BG032300 Rh6BG032600 Rh6BG319500 Rh6CG033400 Rh6CG326000 Rh6DG031100 Rh6DG311900 Rh7AG003400 Rh7CG003000 Rh7CG003100
rosa_wichuraiana Rw2G002170 Rw3G001000 Rw3G002170 Rw4G023880 Rw6G003210 Rw6G027010 Rw7G000280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1285
AccB1I GGYRCC 1 cut(s) 1285
AccBSI CCGCTC 1 cut(s) 1387
AccI GTMKAC 2 cut(s) 1379, 1450
AccII CGCG 1 cut(s) 1416
AccIII TCCGGA 1 cut(s) 1573
AciI CCGC 6 cut(s) 1249, 1303, 1387, 1432, 1473, 1663
AclWI GGATC 1 cut(s) 379
AcoI YGGCCR 3 cut(s) 1129, 1320, 1664
AcsI RAATTY 4 cut(s) 80, 940, 1016, 1364
AcuI CTGAAG 1 cut(s) 537
AfaI GTAC 5 cut(s) 132, 696, 1287, 1344, 1777
AfiI CCNNNNNNNGG 5 cut(s) 1095, 1096, 1258, 1604, 1659
AgsI TTSAA 9 cut(s) 95, 115, 485, 1016, 1076, 1115, 1178, 1711, 1733
AhdI GACNNNNNGTC 1 cut(s) 1496
AjiI CACGTC 1 cut(s) 1060
AjnI CCWGG 3 cut(s) 538, 684, 1669
AluBI AGCT 9 cut(s) 20, 437, 710, 749, 808, 1156, 1189, 1558, 1584
AluI AGCT 9 cut(s) 20, 437, 710, 749, 808, 1156, 1189, 1558, 1584
Alw21I GWGCWC 4 cut(s) 191, 1191, 1424, 1462
Alw26I GTCTC 3 cut(s) 596, 853, 1538
Alw44I GTGCAC 1 cut(s) 1458
AlwI GGATC 1 cut(s) 379
Aor13HI TCCGGA 1 cut(s) 1573
AoxI GGCC 5 cut(s) 641, 1129, 1320, 1664, 1715
ApaLI GTGCAC 1 cut(s) 1458
ApeKI GCWGC 8 cut(s) 168, 464, 704, 707, 1466, 1469, 1555, 1743
ApoI RAATTY 4 cut(s) 80, 940, 1016, 1364
Asp700I GAANNNNTTC 3 cut(s) 288, 942, 980
Asp718I GGTACC 1 cut(s) 1285
AspA2I CCTAGG 1 cut(s) 836
AspLEI GCGC 1 cut(s) 667
AspS9I GGNCC 1 cut(s) 1690
AsuC2I CCSGG 2 cut(s) 234, 1253
AsuHPI GGTGA 2 cut(s) 281, 985
AvaII GGWCC 1 cut(s) 1690
AvrII CCTAGG 1 cut(s) 836
BaeGI GKGCMC 1 cut(s) 1462
BaeI ACNNNNGTAYC 2 cut(s) 1055, 1088
BalI TGGCCA 1 cut(s) 1131
BanI GGYRCC 1 cut(s) 1285
BanII GRGCYC 2 cut(s) 1174, 1191
BbsI GAAGAC 1 cut(s) 1681
Bbv12I GWGCWC 4 cut(s) 191, 1191, 1424, 1462
BbvI GCAGC 8 cut(s) 180, 476, 691, 719, 1453, 1456, 1567, 1730
BccI CCATC 3 cut(s) 1033, 1201, 1297
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BciT130I CCWGG 3 cut(s) 540, 686, 1671
BclI TGATCA 2 cut(s) 403, 1222
BcnI CCSGG 2 cut(s) 234, 1253
BcoDI GTCTC 3 cut(s) 596, 853, 1538
BfaI CTAG 5 cut(s) 333, 837, 900, 1310, 1329
BfmI CTRYAG 4 cut(s) 339, 705, 1008, 1101
BglII AGATCT 1 cut(s) 1047
BlnI CCTAGG 1 cut(s) 836
BlpI GCTNAGC 1 cut(s) 1512
BmcAI AGTACT 1 cut(s) 1344
Bme1390I CCNGG 5 cut(s) 234, 540, 686, 1253, 1671
Bme18I GGWCC 1 cut(s) 1690
BmeRI GACNNNNNGTC 1 cut(s) 1496
BmgBI CACGTC 1 cut(s) 1060
BmgT120I GGNCC 1 cut(s) 1690
BmiI GGNNCC 3 cut(s) 691, 1287, 1596
BmrFI CCNGG 5 cut(s) 234, 540, 686, 1253, 1671
BmsI GCATC 5 cut(s) 323, 620, 706, 1315, 1322
BpiI GAAGAC 1 cut(s) 1681
BplI GAGNNNNNCTC 2 cut(s) 654, 686
Bpu1102I GCTNAGC 1 cut(s) 1512
BpuMI CCSGG 2 cut(s) 234, 1253
BsaI GGTCTC 1 cut(s) 1538
BsaJI CCNNGG 7 cut(s) 206, 365, 538, 685, 836, 910, 1067
BsaWI WCCGGW 1 cut(s) 1573
BsaXI ACNNNNNCTCC 2 cut(s) 405, 435
Bsc4I CCNNNNNNNGG 5 cut(s) 1095, 1096, 1258, 1604, 1659
Bse118I RCCGGY 1 cut(s) 171
Bse1I ACTGG 1 cut(s) 571
Bse3DI GCAATG 3 cut(s) 120, 546, 1323
BseAI TCCGGA 1 cut(s) 1573
BseBI CCWGG 3 cut(s) 540, 686, 1671
BseDI CCNNGG 7 cut(s) 206, 365, 538, 685, 836, 910, 1067
BseGI GGATG 2 cut(s) 786, 1564
BseLI CCNNNNNNNGG 5 cut(s) 1095, 1096, 1258, 1604, 1659
BseMI GCAATG 3 cut(s) 120, 546, 1323
BseMII CTCAG 4 cut(s) 846, 1287, 1374, 1503
BseNI ACTGG 1 cut(s) 571
BseSI GKGCMC 1 cut(s) 1462
BseXI GCAGC 8 cut(s) 180, 476, 691, 719, 1453, 1456, 1567, 1730
BsgI GTGCAG 1 cut(s) 44
Bsh1236I CGCG 1 cut(s) 1416
BshFI GGCC 5 cut(s) 643, 1131, 1322, 1666, 1717
BshNI GGYRCC 1 cut(s) 1285
BsiHKAI GWGCWC 4 cut(s) 191, 1191, 1424, 1462
BsiSI CCGG 4 cut(s) 172, 234, 1252, 1574
BslFI GGGAC 1 cut(s) 216
BslI CCNNNNNNNGG 5 cut(s) 1095, 1096, 1258, 1604, 1659
BsmAI GTCTC 3 cut(s) 596, 853, 1538
BsmBI CGTCTC 1 cut(s) 596
BsmFI GGGAC 1 cut(s) 216
BsmI GAATGC 2 cut(s) 994, 1184
BsnI GGCC 5 cut(s) 643, 1131, 1322, 1666, 1717
Bso31I GGTCTC 1 cut(s) 1538
Bsp1286I GDGCHC 5 cut(s) 191, 1174, 1191, 1424, 1462
Bsp13I TCCGGA 1 cut(s) 1573
Bsp143I GATC 7 cut(s) 7, 142, 163, 384, 403, 1047, 1222
Bsp1720I GCTNAGC 1 cut(s) 1512
Bsp19I CCATGG 3 cut(s) 206, 910, 1067
BspACI CCGC 6 cut(s) 1249, 1303, 1387, 1432, 1473, 1663
BspANI GGCC 5 cut(s) 643, 1131, 1322, 1666, 1717
BspCNI CTCAG 4 cut(s) 847, 1288, 1375, 1504
BspEI TCCGGA 1 cut(s) 1573
BspFNI CGCG 1 cut(s) 1416
BspHI TCATGA 1 cut(s) 1638
BspLI GGNNCC 3 cut(s) 691, 1287, 1596
BspMAI CTGCAG 2 cut(s) 709, 1012
BspPI GGATC 1 cut(s) 379
BspT107I GGYRCC 1 cut(s) 1285
BspTNI GGTCTC 1 cut(s) 1538
BsrBI CCGCTC 1 cut(s) 1387
BsrDI GCAATG 3 cut(s) 120, 546, 1323
BsrFI RCCGGY 1 cut(s) 171
BsrI ACTGG 1 cut(s) 571
BssAI RCCGGY 1 cut(s) 171
BssECI CCNNGG 7 cut(s) 206, 365, 538, 685, 836, 910, 1067
BssMI GATC 7 cut(s) 7, 142, 163, 384, 403, 1047, 1222
BssT1I CCWWGG 4 cut(s) 206, 836, 910, 1067
Bst2UI CCWGG 3 cut(s) 540, 686, 1671
Bst4CI ACNGT 6 cut(s) 253, 369, 515, 590, 608, 1528
Bst6I CTCTTC 1 cut(s) 599
BstAPI GCANNNNNTGC 1 cut(s) 1466
BstC8I GCNNGC 3 cut(s) 312, 435, 1436
BstDEI CTNAG 4 cut(s) 855, 1296, 1383, 1512
BstDSI CCRYGG 4 cut(s) 206, 365, 910, 1067
BstF5I GGATG 2 cut(s) 786, 1564
BstFNI CGCG 1 cut(s) 1416
BstHHI GCGC 1 cut(s) 667
BstKTI GATC 7 cut(s) 10, 145, 166, 387, 406, 1050, 1225
BstMAI GTCTC 3 cut(s) 596, 853, 1538
BstMBI GATC 7 cut(s) 7, 142, 163, 384, 403, 1047, 1222
BstMWI GCNNNNNNNGC 9 cut(s) 271, 439, 716, 998, 1007, 1040, 1428, 1466, 1472
BstNI CCWGG 3 cut(s) 540, 686, 1671
BstNSI RCATGY 4 cut(s) 433, 635, 835, 1466
BstSCI CCNGG 5 cut(s) 232, 538, 684, 1251, 1669
BstSFI CTRYAG 4 cut(s) 339, 705, 1008, 1101
BstSLI GKGCMC 1 cut(s) 1462
BstUI CGCG 1 cut(s) 1416
BstV1I GCAGC 8 cut(s) 180, 476, 691, 719, 1453, 1456, 1567, 1730
BstV2I GAAGAC 1 cut(s) 1681
BstX2I RGATCY 2 cut(s) 384, 1047
BstYI RGATCY 2 cut(s) 384, 1047
BsuRI GGCC 5 cut(s) 643, 1131, 1322, 1666, 1717
BtgI CCRYGG 4 cut(s) 206, 365, 910, 1067
BtrI CACGTC 1 cut(s) 1060
BtsCI GGATG 2 cut(s) 786, 1564
BtsI GCAGTG 2 cut(s) 888, 1005
BtsIMutI CAGTG 4 cut(s) 564, 822, 888, 1005
Cac8I GCNNGC 3 cut(s) 312, 435, 1436
CciI TCATGA 1 cut(s) 1638
CfoI GCGC 1 cut(s) 667
Cfr10I RCCGGY 1 cut(s) 171
Cfr13I GGNCC 1 cut(s) 1690
Csp6I GTAC 5 cut(s) 131, 695, 1286, 1343, 1776
CspCI CAANNNNNGTGG 2 cut(s) 1380, 1415
CviQI GTAC 5 cut(s) 131, 695, 1286, 1343, 1776
DdeI CTNAG 4 cut(s) 855, 1296, 1383, 1512
DpnI GATC 7 cut(s) 9, 144, 165, 386, 405, 1049, 1224
DpnII GATC 7 cut(s) 7, 142, 163, 384, 403, 1047, 1222
DraI TTTAAA 1 cut(s) 958
DriI GACNNNNNGTC 1 cut(s) 1496
EaeI YGGCCR 3 cut(s) 1129, 1320, 1664
Eam1104I CTCTTC 1 cut(s) 599
Eam1105I GACNNNNNGTC 1 cut(s) 1496
EarI CTCTTC 1 cut(s) 599
Ecl136II GAGCTC 1 cut(s) 1189
Eco130I CCWWGG 4 cut(s) 206, 836, 910, 1067
Eco24I GRGCYC 2 cut(s) 1174, 1191
Eco31I GGTCTC 1 cut(s) 1538
Eco32I GATATC 2 cut(s) 397, 1636
Eco47I GGWCC 1 cut(s) 1690
Eco53kI GAGCTC 1 cut(s) 1189
Eco57I CTGAAG 1 cut(s) 537
EcoICRI GAGCTC 1 cut(s) 1189
EcoO109I RGGNCCY 1 cut(s) 1690
EcoRII CCWGG 3 cut(s) 538, 684, 1669
EcoRV GATATC 2 cut(s) 397, 1636
EcoT14I CCWWGG 4 cut(s) 206, 836, 910, 1067
EcoT38I GRGCYC 2 cut(s) 1174, 1191
ErhI CCWWGG 4 cut(s) 206, 836, 910, 1067
Esp3I CGTCTC 1 cut(s) 596
FalI AAGNNNNNCTT 2 cut(s) 535, 567
FaqI GGGAC 1 cut(s) 216
FauNDI CATATG 1 cut(s) 1749
FbaI TGATCA 2 cut(s) 403, 1222
FblI GTMKAC 2 cut(s) 1379, 1450
FokI GGATG 2 cut(s) 793, 1551
FriOI GRGCYC 2 cut(s) 1174, 1191
FspBI CTAG 5 cut(s) 333, 837, 900, 1310, 1329
GlaI GCGC 1 cut(s) 666
HaeIII GGCC 5 cut(s) 643, 1131, 1322, 1666, 1717
HapII CCGG 4 cut(s) 172, 234, 1252, 1574
HhaI GCGC 1 cut(s) 667
Hin6I GCGC 1 cut(s) 665
HinP1I GCGC 1 cut(s) 665
HindIII AAGCTT 2 cut(s) 435, 806
HinfI GANTC 5 cut(s) 32, 352, 1306, 1561, 1657
HpaII CCGG 4 cut(s) 172, 234, 1252, 1574
HphI GGTGA 2 cut(s) 281, 985
Hpy166II GTNNAC 5 cut(s) 820, 1380, 1451, 1460, 1490
Hpy188I TCNGA 3 cut(s) 163, 796, 856
Hpy8I GTNNAC 5 cut(s) 820, 1380, 1451, 1460, 1490
Hpy99I CGWCG 2 cut(s) 773, 1607
HpyCH4III ACNGT 6 cut(s) 253, 369, 515, 590, 608, 1528
HpyCH4IV ACGT 2 cut(s) 454, 1059
HpyF10VI GCNNNNNNNGC 9 cut(s) 271, 439, 716, 998, 1007, 1040, 1428, 1466, 1472
HpyF3I CTNAG 4 cut(s) 855, 1296, 1383, 1512
HpySE526I ACGT 2 cut(s) 454, 1059
HspAI GCGC 1 cut(s) 665
Kpn2I TCCGGA 1 cut(s) 1573
KpnI GGTACC 1 cut(s) 1289
Ksp22I TGATCA 2 cut(s) 403, 1222
Kzo9I GATC 7 cut(s) 7, 142, 163, 384, 403, 1047, 1222
LmnI GCTCC 5 cut(s) 194, 1194, 1210, 1581, 1594
Lsp1109I GCAGC 8 cut(s) 180, 476, 691, 719, 1453, 1456, 1567, 1730
LweI GCATC 5 cut(s) 323, 620, 706, 1315, 1322
MaeI CTAG 5 cut(s) 333, 837, 900, 1310, 1329
MaeII ACGT 2 cut(s) 454, 1059
MaeIII GTNAC 2 cut(s) 1147, 1535
MalI GATC 7 cut(s) 9, 144, 165, 386, 405, 1049, 1224
MbiI CCGCTC 1 cut(s) 1387
MboI GATC 7 cut(s) 7, 142, 163, 384, 403, 1047, 1222
MflI RGATCY 2 cut(s) 384, 1047
MhlI GDGCHC 5 cut(s) 191, 1174, 1191, 1424, 1462
MlsI TGGCCA 1 cut(s) 1131
MluNI TGGCCA 1 cut(s) 1131
MlyI GAGTC 1 cut(s) 1651
MmeI TCCRAC 2 cut(s) 266, 1250
MnlI CCTC 7 cut(s) 680, 747, 871, 1095, 1306, 1606, 1707
Mox20I TGGCCA 1 cut(s) 1131
MroI TCCGGA 1 cut(s) 1573
MroXI GAANNNNTTC 3 cut(s) 288, 942, 980
MscI TGGCCA 1 cut(s) 1131
MseI TTAA 1 cut(s) 957
MslI CAYNNNNRTG 3 cut(s) 279, 1218, 1443
Msp20I TGGCCA 1 cut(s) 1131
MspA1I CMGCKG 3 cut(s) 710, 1434, 1558
MspI CCGG 4 cut(s) 172, 234, 1252, 1574
MspR9I CCNGG 5 cut(s) 234, 540, 686, 1253, 1671
Mva1269I GAATGC 2 cut(s) 994, 1184
MvaI CCWGG 3 cut(s) 540, 686, 1671
MvnI CGCG 1 cut(s) 1416
MwoI GCNNNNNNNGC 9 cut(s) 271, 439, 716, 998, 1007, 1040, 1428, 1466, 1472
NciI CCSGG 2 cut(s) 234, 1253
NcoI CCATGG 3 cut(s) 206, 910, 1067
NdeI CATATG 1 cut(s) 1749
NdeII GATC 7 cut(s) 7, 142, 163, 384, 403, 1047, 1222
NlaIV GGNNCC 3 cut(s) 691, 1287, 1596
NmeAIII GCCGAG 1 cut(s) 1639
NspI RCATGY 4 cut(s) 433, 635, 835, 1466
PagI TCATGA 1 cut(s) 1638
PctI GAATGC 2 cut(s) 994, 1184
PdmI GAANNNNTTC 3 cut(s) 288, 942, 980
PfeI GAWTC 4 cut(s) 32, 352, 1306, 1561
PfoI TCCNGGA 1 cut(s) 232
PleI GAGTC 1 cut(s) 1651
PpsI GAGTC 1 cut(s) 1651
PpuMI RGGWCCY 1 cut(s) 1690
Psp124BI GAGCTC 1 cut(s) 1191
Psp5II RGGWCCY 1 cut(s) 1690
Psp6I CCWGG 3 cut(s) 538, 684, 1669
PspGI CCWGG 3 cut(s) 538, 684, 1669
PspN4I GGNNCC 3 cut(s) 691, 1287, 1596
PspPI GGNCC 1 cut(s) 1690
PspPPI RGGWCCY 1 cut(s) 1690
PstI CTGCAG 2 cut(s) 709, 1012
PsuI RGATCY 2 cut(s) 384, 1047
PvuII CAGCTG 2 cut(s) 710, 1558
RsaI GTAC 5 cut(s) 132, 696, 1287, 1344, 1777
RsaNI GTAC 5 cut(s) 131, 695, 1286, 1343, 1776
RseI CAYNNNNRTG 3 cut(s) 279, 1218, 1443
SacI GAGCTC 1 cut(s) 1191
SaqAI TTAA 1 cut(s) 957
Sau3AI GATC 7 cut(s) 7, 142, 163, 384, 403, 1047, 1222
Sau96I GGNCC 1 cut(s) 1690
ScaI AGTACT 1 cut(s) 1344
SchI GAGTC 1 cut(s) 1651
ScrFI CCNGG 5 cut(s) 234, 540, 686, 1253, 1671
SduI GDGCHC 5 cut(s) 191, 1174, 1191, 1424, 1462
SfaNI GCATC 5 cut(s) 323, 620, 706, 1315, 1322
SfcI CTRYAG 4 cut(s) 339, 705, 1008, 1101
SinI GGWCC 1 cut(s) 1690
SmiMI CAYNNNNRTG 3 cut(s) 279, 1218, 1443
SsiI CCGC 6 cut(s) 1249, 1303, 1387, 1432, 1473, 1663
SspMI CTAG 5 cut(s) 333, 837, 900, 1310, 1329
SstI GAGCTC 1 cut(s) 1191
StyD4I CCNGG 5 cut(s) 232, 538, 684, 1251, 1669
StyI CCWWGG 4 cut(s) 206, 836, 910, 1067
TaaI ACNGT 6 cut(s) 253, 369, 515, 590, 608, 1528
TaiI ACGT 2 cut(s) 457, 1062
TaqI TCGA 1 cut(s) 1362
TatI WGTACW 2 cut(s) 130, 1342
TauI GCSGC 3 cut(s) 1434, 1475, 1666
TfiI GAWTC 4 cut(s) 32, 352, 1306, 1561
Tru1I TTAA 1 cut(s) 957
Tru9I TTAA 1 cut(s) 957
TscAI CASTG 4 cut(s) 571, 829, 888, 1012
TseI GCWGC 8 cut(s) 168, 464, 704, 707, 1466, 1469, 1555, 1743
TspDTI ATGAA 5 cut(s) 290, 461, 586, 1371, 1553
TspGWI ACGGA 5 cut(s) 62, 364, 389, 682, 1620
TspRI CASTG 4 cut(s) 571, 829, 888, 1012
VneI GTGCAC 1 cut(s) 1458
VpaK11BI GGWCC 1 cut(s) 1690
XapI RAATTY 4 cut(s) 80, 940, 1016, 1364
XbaI TCTAGA 1 cut(s) 1309
XceI RCATGY 4 cut(s) 433, 635, 835, 1466
XmaJI CCTAGG 1 cut(s) 836
XmiI GTMKAC 2 cut(s) 1379, 1450
XmnI GAANNNNTTC 3 cut(s) 288, 942, 980
XspI CTAG 5 cut(s) 333, 837, 900, 1310, 1329
ZrmI AGTACT 1 cut(s) 1344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.